ES2762677T3 - Métodos para aislar microvesículas y extracción de ácidos nucleicos de muestras biológicas - Google Patents

Métodos para aislar microvesículas y extracción de ácidos nucleicos de muestras biológicas Download PDF

Info

Publication number
ES2762677T3
ES2762677T3 ES15818561T ES15818561T ES2762677T3 ES 2762677 T3 ES2762677 T3 ES 2762677T3 ES 15818561 T ES15818561 T ES 15818561T ES 15818561 T ES15818561 T ES 15818561T ES 2762677 T3 ES2762677 T3 ES 2762677T3
Authority
ES
Spain
Prior art keywords
dna
rna
biological sample
isolation
capture surface
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES15818561T
Other languages
English (en)
Spanish (es)
Inventor
Johan Karl Olov Skog
Daniel Enderle
Aparna Ramachandran
Haoheng Yan
Emily Berghoff
Tai-Fen Wei
Mikkel Noerholm
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Exosome Diagnostics Inc
Original Assignee
Exosome Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Exosome Diagnostics Inc filed Critical Exosome Diagnostics Inc
Application granted granted Critical
Publication of ES2762677T3 publication Critical patent/ES2762677T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149Particles, e.g. beads

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Tires In General (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
ES15818561T 2014-07-09 2015-07-09 Métodos para aislar microvesículas y extracción de ácidos nucleicos de muestras biológicas Active ES2762677T3 (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201462022538P 2014-07-09 2014-07-09
US201462079763P 2014-11-14 2014-11-14
US201562166890P 2015-05-27 2015-05-27
PCT/US2015/039760 WO2016007755A1 (en) 2014-07-09 2015-07-09 Methods for isolating microvesicles and extracting nucleic acids from biological samples

Publications (1)

Publication Number Publication Date
ES2762677T3 true ES2762677T3 (es) 2020-05-25

Family

ID=55064901

Family Applications (2)

Application Number Title Priority Date Filing Date
ES15818561T Active ES2762677T3 (es) 2014-07-09 2015-07-09 Métodos para aislar microvesículas y extracción de ácidos nucleicos de muestras biológicas
ES19201765T Active ES2898254T3 (es) 2014-07-09 2015-07-09 Métodos para aislar microvesículas y extraer ácidos nucleicos de muestras biológicas

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES19201765T Active ES2898254T3 (es) 2014-07-09 2015-07-09 Métodos para aislar microvesículas y extraer ácidos nucleicos de muestras biológicas

Country Status (11)

Country Link
US (1) US10465183B2 (enExample)
EP (2) EP3623792B1 (enExample)
JP (2) JP6759182B2 (enExample)
KR (2) KR102421185B1 (enExample)
CN (2) CN107002075B (enExample)
AU (2) AU2015287763B2 (enExample)
CA (2) CA3084920C (enExample)
ES (2) ES2762677T3 (enExample)
IL (2) IL250000B (enExample)
SG (1) SG11201700146QA (enExample)
WO (1) WO2016007755A1 (enExample)

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3569607A1 (en) * 2011-05-11 2019-11-20 Exosome Diagnostics, Inc. Nucleic acid extraction from heterogeneous biological materials
US11268085B2 (en) 2014-05-27 2022-03-08 Exosome Diagnostics, Inc. Methods for isolating microvesicles and extracting nucleic acids from biological samples
CA3084920C (en) 2014-07-09 2023-02-28 Exosome Diagnostics, Inc. Methods for isolating microvesicles and extracting nucleic acids from biological samples
CN105821031A (zh) * 2016-01-18 2016-08-03 昆明医科大学 一种二氯甲烷法提取dna的原理
KR20190020649A (ko) 2016-04-15 2019-03-04 엑소좀 디아그노스틱스, 인크. 역형성 림프종 키나아제(alk) 핵산 및 alk 융합 전사물의 혈장 기반 검출 및 암의 진단 및 치료에 있어 이의 용도
WO2017185086A1 (en) 2016-04-22 2017-10-26 Exosome Diagnostics, Inc. Devices and methods for in vivo capture of biological samples and nucleic acids therein
KR102741603B1 (ko) 2016-05-02 2024-12-16 엔코디아, 인코포레이티드 암호화 핵산을 사용한 거대분자 분석
ES2908932T3 (es) 2016-05-05 2022-05-04 Exosome Diagnostics Inc Perfilado de ácidos nucleicos de microvesícula y usos del mismo como características distintivas en diagnóstico de rechazo de trasplante renal
AU2017263886B2 (en) * 2016-05-13 2023-11-09 Exosome Diagnostics, Inc. Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids
CA3033650A1 (en) 2016-08-17 2018-02-22 The Regents Of The University Of California A novel immunoprobe-based method to assess organ injury status through a biofluid-based cell-free dna (cfdna) assay
CN109689869B (zh) 2016-09-14 2022-09-27 东丽株式会社 无细胞dna的回收方法
CN110191962A (zh) * 2016-10-21 2019-08-30 外来体诊断公司 外来体相关核酸的测序和分析
WO2018102162A1 (en) 2016-11-30 2018-06-07 Exosome Diagnostics, Inc. Methods and compositions to detect mutations in plasma using exosomal rna and cell free dna from non-small cell lung cancer patients
RU2748234C2 (ru) * 2016-12-23 2021-05-21 Эксофарм Лтд Способы и композиции для очистки или выделения микровезикул и экзосом
US20200149036A1 (en) 2017-01-02 2020-05-14 Exosome Diagnostics, Inc. Methods to distinguish rna and dna in a combined preparation
CA3048212A1 (en) * 2017-01-06 2018-07-12 Mantra Bio, Inc. Systems and methods for algorithmic extracellular vesicle population discovery and characterization
KR101875594B1 (ko) * 2017-01-13 2018-07-06 ㈜로제타엑소좀 금속을 이용한 세포밖 소포체의 분리 방법
GB201706680D0 (en) * 2017-04-27 2017-06-14 Ge Healthcare Uk Ltd Device and method for sample isolation
US11891664B2 (en) 2017-05-17 2024-02-06 Exosome Diagnostics, Inc. Methods of detecting renal transplant rejection using microvesicle nucleic acid biomarkers
CN107267440A (zh) * 2017-06-09 2017-10-20 李刚 适用于外泌体提取的提取试剂及应用及其提取方法
US11390864B2 (en) 2017-07-12 2022-07-19 Illumina, Inc. Nucleic acid extraction materials, systems, and methods
WO2019014486A1 (en) 2017-07-12 2019-01-17 Exosome Diagnostics, Inc. METHODS FOR ISOLATING AND ENRICHING POPULATIONS OF EXTRACELLULAR VESICLES DERIVED FROM BIOFLUIDS, AND METHODS OF USE THEREOF
EP3655531B1 (en) * 2017-07-18 2024-09-04 Exosome Diagnostics, Inc. Sequencing of nucleic acids associated with exosomal isolation from patients with glioblastoma multiforme
EP3660142A4 (en) * 2017-07-26 2021-05-05 Rosetta Exosome METHOD OF ISOLATION OF EXTRACELLULAR VESICLES USING CATIONS
CN111386122A (zh) * 2017-09-20 2020-07-07 分子听诊器公司 用于检测组织状况的方法和系统
WO2019089836A1 (en) 2017-10-31 2019-05-09 Encodia, Inc. Kits for analysis using nucleic acid encoding and/or label
US11260347B2 (en) * 2017-12-26 2022-03-01 Limited Liability Company “Prostagnost” Method and device for separating extracellular vesicles from biological liquids with the aid of cascade ultrafiltration
US12429465B2 (en) 2018-02-20 2025-09-30 Korea University Research And Business Foundation Multi-column for isolating exosomes and exosome isolation method
US12378608B2 (en) 2018-06-06 2025-08-05 Exosome Diagnostics, Inc. Methods for developing urine biomarkers and for detecting bladder cancer
BR112020026067A2 (pt) 2018-06-18 2021-03-23 Exopharm Limited métodos e composições para purificação ou isolamento de microvesículas e exossomas
CN108841777A (zh) * 2018-06-22 2018-11-20 北京恩泽康泰生物科技有限公司 基于静电吸附的胞外囊泡及其内含物的提取方法及装置
JP7426725B2 (ja) * 2018-10-30 2024-02-02 Craif株式会社 細胞外小胞を捕捉するために用いられるデバイス、細胞外小胞の保存方法および移送方法
US12258587B2 (en) 2018-11-20 2025-03-25 Exosome Diagnostics, Inc. Compositions and methods for internal controls of microvesicle isolations
JP7582676B2 (ja) 2019-04-30 2024-11-13 エンコディア, インコーポレイテッド 分析物を調製するための方法および関連キット
JP7719052B2 (ja) * 2019-08-08 2025-08-05 バイオカルティス エン フェー 新規の核酸精製の化学
EP4031865A2 (en) 2019-09-18 2022-07-27 Exosome Diagnostics, Inc. Compositions, methods, and kits for the isolation of extracellular vesicles
JP2023501354A (ja) * 2019-11-04 2023-01-18 ナサスビオテック、ソシエダッド、リミターダ 核酸の単離方法
EP4077660A1 (en) * 2019-12-16 2022-10-26 QIAGEN GmbH Method for enriching vesicular rna
WO2021122846A1 (en) * 2019-12-16 2021-06-24 Qiagen Gmbh Enrichment method
CN115698265A (zh) * 2020-04-24 2023-02-03 高丽大学校算学协力团 微囊泡分离方法及微囊泡分离装置
WO2021215539A1 (ja) * 2020-04-24 2021-10-28 東洋紡株式会社 イオン交換膜
CA3180572A1 (en) 2020-05-29 2021-12-02 Johan Skog Use of microvesicle signature for the diagnosis and treatment of kidney transplant rejection
EP4263383A4 (en) 2020-12-17 2024-10-23 Nephrosant, Inc. KITS FOR STABILIZATION OF URINE SAMPLES
WO2022263500A1 (en) * 2021-06-17 2022-12-22 Qiagen Gmbh Method for isolating non-vesicular mirna
EP4370712A1 (en) 2021-07-16 2024-05-22 Exosome Diagnostics, Inc. Methods of detecting sjögren's syndrome using salivary exosomes
DE102021208893B3 (de) 2021-08-13 2022-11-17 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Isolieren von Analyten unterschiedlicher Analyt-Klassen
EP4419911A4 (en) * 2021-10-28 2025-03-05 10X Genomics, Inc. Methods for sample preparation
AU2023221644A1 (en) 2022-02-18 2024-09-12 Bio-Techne Corporation Use of microvesicle signatures in the identification and treatment of renal disorders
EP4584397A1 (en) 2022-09-07 2025-07-16 Exosome Diagnostics, Inc. Methods of detecting sjögren's syndrome using salivary exosomes
CN115960885B (zh) * 2022-10-09 2023-12-12 南京诺唯赞生物科技股份有限公司 一种提取肝素钠样品中核酸的方法及组合物
KR102784335B1 (ko) * 2023-02-09 2025-03-19 순천향대학교 산학협력단 세포유리핵산의 고농축화 분리 방법

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4935342A (en) 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
US5438128A (en) * 1992-02-07 1995-08-01 Millipore Corporation Method for rapid purifiction of nucleic acids using layered ion-exchange membranes
US5639606A (en) 1993-04-06 1997-06-17 The University Of Rochester Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
EP0723549B1 (en) * 1993-08-30 2003-12-17 Promega Corporation Nucleic acid purification compositions and methods
FR2788780B1 (fr) * 1999-01-27 2001-03-30 Ap Cells Inc Procede de preparation de vesicules membranaires
GB9927320D0 (en) 1999-11-18 2000-01-12 Chiron Spa Exosome separation
US6812023B1 (en) 2000-04-27 2004-11-02 Anosys, Inc. Methods of producing membrane vesicles
EP2081442B1 (en) * 2006-10-10 2016-08-10 TrovaGene, Inc. Compositions, methods and kits for isolating nucleic acids from body fluids using anion exchange media
KR101970908B1 (ko) 2008-02-01 2019-04-19 더 제너럴 하스피탈 코포레이션 의학적 질환 및 병태의 진단, 예후, 및 치료에 있어서 미세소포체의 용도
WO2010065765A2 (en) * 2008-12-04 2010-06-10 Aethlon Medical, Inc. Affinity capture of circulating biomarkers
US20120077263A1 (en) * 2009-06-05 2012-03-29 Mayo Foundation For Medical Education And Research Methods and materials for isolating exosomes
WO2011151428A1 (en) 2010-06-01 2011-12-08 Qiagen Gmbh Method for isolating and/or purifying nucleic acid(s)
WO2012006476A2 (en) * 2010-07-07 2012-01-12 Aethlon Medical, Inc. Methods and compositions for quantifying exosomes
WO2012024695A1 (en) * 2010-08-20 2012-02-23 Life Technologies Corporation Magnetic beads having surface glycoconjugates and use thereof
EP2638057B1 (en) * 2010-11-10 2019-03-06 Exosome Diagnostics, Inc. Method for isolation of nucleic acid containing particles and extraction of nucleic acids therefrom
SG190450A1 (en) * 2010-12-20 2013-06-28 Agency Science Tech & Res Method of purifying exosomes
EP3569607A1 (en) * 2011-05-11 2019-11-20 Exosome Diagnostics, Inc. Nucleic acid extraction from heterogeneous biological materials
WO2012174282A2 (en) * 2011-06-16 2012-12-20 Caris Life Sciences Luxembourg Holdings, S.A.R.L. Biomarker compositions and methods
KR20140005688A (ko) * 2012-07-06 2014-01-15 삼성전자주식회사 사용자 인터페이스 방법 및 장치
WO2014036391A1 (en) 2012-08-30 2014-03-06 Exosome Diagnostics, Inc. Controls for nucleic acid assays
KR101933621B1 (ko) * 2012-09-28 2018-12-28 삼성전자주식회사 소포를 분리하기 위한 조성물, 키트 및 이를 이용하여 소포를 분리하는 방법
CN105026911B (zh) 2013-01-03 2019-01-22 外来体诊断公司 用于分离微囊泡的方法
CA3084920C (en) 2014-07-09 2023-02-28 Exosome Diagnostics, Inc. Methods for isolating microvesicles and extracting nucleic acids from biological samples

Also Published As

Publication number Publication date
AU2019250221B2 (en) 2021-05-06
EP3167062B1 (en) 2019-10-09
CN113699143A (zh) 2021-11-26
JP2017520264A (ja) 2017-07-27
US20170198280A1 (en) 2017-07-13
IL250000B (en) 2021-02-28
JP6759182B2 (ja) 2020-09-23
JP2020202860A (ja) 2020-12-24
KR102421185B1 (ko) 2022-07-14
AU2019250221A1 (en) 2019-11-07
US10465183B2 (en) 2019-11-05
JP7128866B2 (ja) 2022-08-31
WO2016007755A1 (en) 2016-01-14
KR20170028432A (ko) 2017-03-13
EP3167062A1 (en) 2017-05-17
CA3084920A1 (en) 2016-01-14
IL280307A (en) 2021-03-01
AU2015287763A1 (en) 2017-02-23
EP3623792A1 (en) 2020-03-18
AU2015287763B2 (en) 2019-08-08
EP3167062A4 (en) 2017-12-06
IL250000A0 (en) 2017-03-30
ES2898254T3 (es) 2022-03-04
IL280307B (en) 2022-02-01
CN113699143B (zh) 2024-08-06
CN107002075A (zh) 2017-08-01
KR20220105172A (ko) 2022-07-26
CA2954576A1 (en) 2016-01-14
CN107002075B (zh) 2021-07-30
EP3623792B1 (en) 2021-09-01
CA2954576C (en) 2020-08-11
KR102596577B1 (ko) 2023-10-30
SG11201700146QA (en) 2017-02-27
CA3084920C (en) 2023-02-28

Similar Documents

Publication Publication Date Title
ES2762677T3 (es) Métodos para aislar microvesículas y extracción de ácidos nucleicos de muestras biológicas
JP7354327B2 (ja) 生体液からの細胞外小胞の単離及びセルフリーdnaの同時単離のための自動及び手動方法
JP6449781B2 (ja) 微細小胞の単離方法
ES2908932T3 (es) Perfilado de ácidos nucleicos de microvesícula y usos del mismo como características distintivas en diagnóstico de rechazo de trasplante renal
US11268085B2 (en) Methods for isolating microvesicles and extracting nucleic acids from biological samples
CA3022528C (en) Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free dna from biofluids
HK1238676A1 (en) Methods for isolating microvesicles and extracting nucleic acids from biological samples