ES2762677T3 - Métodos para aislar microvesículas y extracción de ácidos nucleicos de muestras biológicas - Google Patents

Métodos para aislar microvesículas y extracción de ácidos nucleicos de muestras biológicas Download PDF

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Publication number
ES2762677T3
ES2762677T3 ES15818561T ES15818561T ES2762677T3 ES 2762677 T3 ES2762677 T3 ES 2762677T3 ES 15818561 T ES15818561 T ES 15818561T ES 15818561 T ES15818561 T ES 15818561T ES 2762677 T3 ES2762677 T3 ES 2762677T3
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dna
rna
biological sample
isolation
capture surface
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Johan Karl Olov Skog
Daniel Enderle
Aparna Ramachandran
Haoheng Yan
Emily Berghoff
Tai-Fen Wei
Mikkel Noerholm
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Exosome Diagnostics Inc
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Exosome Diagnostics Inc
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149Particles, e.g. beads

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Tires In General (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
ES15818561T 2014-07-09 2015-07-09 Métodos para aislar microvesículas y extracción de ácidos nucleicos de muestras biológicas Active ES2762677T3 (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201462022538P 2014-07-09 2014-07-09
US201462079763P 2014-11-14 2014-11-14
US201562166890P 2015-05-27 2015-05-27
PCT/US2015/039760 WO2016007755A1 (en) 2014-07-09 2015-07-09 Methods for isolating microvesicles and extracting nucleic acids from biological samples

Publications (1)

Publication Number Publication Date
ES2762677T3 true ES2762677T3 (es) 2020-05-25

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ES15818561T Active ES2762677T3 (es) 2014-07-09 2015-07-09 Métodos para aislar microvesículas y extracción de ácidos nucleicos de muestras biológicas
ES19201765T Active ES2898254T3 (es) 2014-07-09 2015-07-09 Métodos para aislar microvesículas y extraer ácidos nucleicos de muestras biológicas

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ES19201765T Active ES2898254T3 (es) 2014-07-09 2015-07-09 Métodos para aislar microvesículas y extraer ácidos nucleicos de muestras biológicas

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Country Link
US (1) US10465183B2 (enExample)
EP (2) EP3623792B1 (enExample)
JP (2) JP6759182B2 (enExample)
KR (2) KR102596577B1 (enExample)
CN (2) CN107002075B (enExample)
AU (2) AU2015287763B2 (enExample)
CA (2) CA2954576C (enExample)
ES (2) ES2762677T3 (enExample)
IL (2) IL250000B (enExample)
SG (1) SG11201700146QA (enExample)
WO (1) WO2016007755A1 (enExample)

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Publication number Publication date
US10465183B2 (en) 2019-11-05
EP3623792B1 (en) 2021-09-01
AU2015287763A1 (en) 2017-02-23
AU2019250221B2 (en) 2021-05-06
JP2017520264A (ja) 2017-07-27
AU2019250221A1 (en) 2019-11-07
CA2954576C (en) 2020-08-11
CN107002075A (zh) 2017-08-01
IL280307B (en) 2022-02-01
EP3167062A4 (en) 2017-12-06
CA3084920A1 (en) 2016-01-14
AU2015287763B2 (en) 2019-08-08
CA3084920C (en) 2023-02-28
KR20170028432A (ko) 2017-03-13
WO2016007755A1 (en) 2016-01-14
CA2954576A1 (en) 2016-01-14
CN107002075B (zh) 2021-07-30
IL280307A (en) 2021-03-01
JP2020202860A (ja) 2020-12-24
EP3167062B1 (en) 2019-10-09
US20170198280A1 (en) 2017-07-13
ES2898254T3 (es) 2022-03-04
IL250000A0 (en) 2017-03-30
SG11201700146QA (en) 2017-02-27
IL250000B (en) 2021-02-28
KR102596577B1 (ko) 2023-10-30
CN113699143A (zh) 2021-11-26
EP3167062A1 (en) 2017-05-17
JP6759182B2 (ja) 2020-09-23
EP3623792A1 (en) 2020-03-18
JP7128866B2 (ja) 2022-08-31
KR102421185B1 (ko) 2022-07-14
KR20220105172A (ko) 2022-07-26
CN113699143B (zh) 2024-08-06

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