AU2015287763B2 - Methods for isolating microvesicles and extracting nucleic acids from biological samples - Google Patents
Methods for isolating microvesicles and extracting nucleic acids from biological samples Download PDFInfo
- Publication number
- AU2015287763B2 AU2015287763B2 AU2015287763A AU2015287763A AU2015287763B2 AU 2015287763 B2 AU2015287763 B2 AU 2015287763B2 AU 2015287763 A AU2015287763 A AU 2015287763A AU 2015287763 A AU2015287763 A AU 2015287763A AU 2015287763 B2 AU2015287763 B2 AU 2015287763B2
- Authority
- AU
- Australia
- Prior art keywords
- dna
- rna
- biological sample
- membrane
- isolation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/149—Particles, e.g. beads
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Tires In General (AREA)
- Mechanical Treatment Of Semiconductor (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2019250221A AU2019250221B2 (en) | 2014-07-09 | 2019-10-17 | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462022538P | 2014-07-09 | 2014-07-09 | |
| US62/022,538 | 2014-07-09 | ||
| US201462079763P | 2014-11-14 | 2014-11-14 | |
| US62/079,763 | 2014-11-14 | ||
| US201562166890P | 2015-05-27 | 2015-05-27 | |
| US62/166,890 | 2015-05-27 | ||
| PCT/US2015/039760 WO2016007755A1 (en) | 2014-07-09 | 2015-07-09 | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2019250221A Division AU2019250221B2 (en) | 2014-07-09 | 2019-10-17 | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2015287763A1 AU2015287763A1 (en) | 2017-02-23 |
| AU2015287763B2 true AU2015287763B2 (en) | 2019-08-08 |
Family
ID=55064901
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2015287763A Active AU2015287763B2 (en) | 2014-07-09 | 2015-07-09 | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
| AU2019250221A Active AU2019250221B2 (en) | 2014-07-09 | 2019-10-17 | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2019250221A Active AU2019250221B2 (en) | 2014-07-09 | 2019-10-17 | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US10465183B2 (enExample) |
| EP (2) | EP3623792B1 (enExample) |
| JP (2) | JP6759182B2 (enExample) |
| KR (2) | KR102421185B1 (enExample) |
| CN (2) | CN113699143B (enExample) |
| AU (2) | AU2015287763B2 (enExample) |
| CA (2) | CA2954576C (enExample) |
| ES (2) | ES2762677T3 (enExample) |
| IL (2) | IL250000B (enExample) |
| SG (1) | SG11201700146QA (enExample) |
| WO (1) | WO2016007755A1 (enExample) |
Families Citing this family (51)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2012155014A1 (en) * | 2011-05-11 | 2012-11-15 | Exosome Diagnostics, Inc. | Nucleic acid extraction from heterogeneous biological materials |
| US11268085B2 (en) | 2014-05-27 | 2022-03-08 | Exosome Diagnostics, Inc. | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
| SG11201700146QA (en) | 2014-07-09 | 2017-02-27 | Exosome Diagnostics Inc | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
| CN105821031A (zh) * | 2016-01-18 | 2016-08-03 | 昆明医科大学 | 一种二氯甲烷法提取dna的原理 |
| SG11201809005TA (en) | 2016-04-15 | 2018-11-29 | Exosome Diagnostics Inc | Plasma-based detection of anaplastic lymphoma kinase (alk) nucleic acids and alk fusion transcripts and uses thereof in diagnosis and treatment of cancer |
| WO2017185086A1 (en) | 2016-04-22 | 2017-10-26 | Exosome Diagnostics, Inc. | Devices and methods for in vivo capture of biological samples and nucleic acids therein |
| CA3022863A1 (en) | 2016-05-02 | 2017-11-09 | Encodia, Inc. | Macromolecule analysis employing nucleic acid encoding of molecular recognition events |
| EP3452613B1 (en) | 2016-05-05 | 2021-12-29 | Exosome Diagnostics, Inc. | Profiling microvesicle nucleic acids and uses thereof as signatures in diagnosis of renal transplant rejection |
| AU2017263886B2 (en) * | 2016-05-13 | 2023-11-09 | Exosome Diagnostics, Inc. | Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids |
| EP3845665A1 (en) | 2016-08-17 | 2021-07-07 | The Regents Of The University Of California | A novel immunoprobe-based method to assess organ injury status through a biofluid-based cell-free dna (cfdna) assay |
| US20190203200A1 (en) | 2016-09-14 | 2019-07-04 | Toray Industries, Inc. | Method of collecting cell-free dna |
| EP3529374B1 (en) | 2016-10-21 | 2024-04-03 | Exosome Diagnostics, Inc. | Sequencing and analysis of exosome associated nucleic acids |
| CN110446790B (zh) | 2016-11-30 | 2023-03-31 | 外来体诊断公司 | 使用外来体rna和无细胞dna检测血浆中的突变的方法和组合物 |
| JP7376091B2 (ja) * | 2016-12-23 | 2023-11-08 | エクソファーマ リミテッド | 微小胞およびエキソソームの精製または単離のための方法および組成物 |
| WO2018126278A2 (en) | 2017-01-02 | 2018-07-05 | Exosome Diagnostics, Inc. | Methods to distinguish rna and dna in a combined preparation |
| US11830583B2 (en) * | 2017-01-06 | 2023-11-28 | Mantra Bio Inc. | Systems and methods for characterizing extracellular vesicle (EV) population by predicting whether the EV originates from B, T, or dendritic cells |
| KR101875594B1 (ko) * | 2017-01-13 | 2018-07-06 | ㈜로제타엑소좀 | 금속을 이용한 세포밖 소포체의 분리 방법 |
| GB201706680D0 (en) * | 2017-04-27 | 2017-06-14 | Ge Healthcare Uk Ltd | Device and method for sample isolation |
| WO2018213392A1 (en) | 2017-05-17 | 2018-11-22 | Exosome Diagnostics, Inc. | Microvesicle nucleic acids and/or proteins and uses thereof as markers of renal transplant rejection |
| CN107267440A (zh) * | 2017-06-09 | 2017-10-20 | 李刚 | 适用于外泌体提取的提取试剂及应用及其提取方法 |
| WO2019013991A2 (en) | 2017-07-12 | 2019-01-17 | Illumina, Inc. | METHODS, SYSTEMS AND MATERIALS FOR EXTRACTING NUCLEIC ACIDS |
| CN111133106A (zh) | 2017-07-12 | 2020-05-08 | 外来体诊断公司 | 用于分离和富集生物流体来源的细胞外囊泡的方法及其使用方法 |
| EP3655531B1 (en) * | 2017-07-18 | 2024-09-04 | Exosome Diagnostics, Inc. | Sequencing of nucleic acids associated with exosomal isolation from patients with glioblastoma multiforme |
| JP2020528766A (ja) * | 2017-07-26 | 2020-10-01 | ロゼッタ エクソソーム | 陽イオンを利用した細胞外小胞体の分離方法 |
| SG11202003588QA (en) * | 2017-09-20 | 2020-05-28 | Molecular Stethoscope Inc | Methods and systems for detecting tissue conditions |
| JP7390027B2 (ja) | 2017-10-31 | 2023-12-01 | エンコディア， インコーポレイテッド | 核酸エンコーディングおよび／または標識を使用する解析のためのキット |
| US11260347B2 (en) * | 2017-12-26 | 2022-03-01 | Limited Liability Company “Prostagnost” | Method and device for separating extracellular vesicles from biological liquids with the aid of cascade ultrafiltration |
| CN111770931B (zh) * | 2018-02-20 | 2023-08-25 | 高丽大学校产学协力团 | 用于分离外泌体的多柱及外泌体分离方法 |
| US12378608B2 (en) | 2018-06-06 | 2025-08-05 | Exosome Diagnostics, Inc. | Methods for developing urine biomarkers and for detecting bladder cancer |
| CA3104349A1 (en) | 2018-06-18 | 2019-12-26 | Exopharm Limited | Methods and compositions for purification or isolation of microvesicles and exosomes |
| CN108841777A (zh) * | 2018-06-22 | 2018-11-20 | 北京恩泽康泰生物科技有限公司 | 基于静电吸附的胞外囊泡及其内含物的提取方法及装置 |
| EP3875570A4 (en) * | 2018-10-30 | 2022-08-24 | Craif Inc. | DEVICE USED FOR CONTAINING EXTRACELLULAR VESICLES AND METHODS FOR PRESERVING AND TRANSPORTING EXTRACELLULAR VESICLES |
| EP3884045B1 (en) | 2018-11-20 | 2023-01-11 | Exosome Diagnostics, Inc. | Methods for internal controls of microvesicle isolations |
| EP3963070A4 (en) | 2019-04-30 | 2023-02-22 | Encodia, Inc. | METHODS OF PREPARATION OF ANALYTES AND RELATED KITS |
| WO2021023854A1 (en) * | 2019-08-08 | 2021-02-11 | Biocartis Nv | Novel nucleic acid purification chemistry |
| WO2021055770A2 (en) | 2019-09-18 | 2021-03-25 | Exosome Diagnostics, Inc. | Compositions, methods, and kits for the isolation of extracellular vesicles |
| JP2023501354A (ja) * | 2019-11-04 | 2023-01-18 | ナサスビオテック、ソシエダッド、リミターダ | 核酸の単離方法 |
| EP4077659A1 (en) * | 2019-12-16 | 2022-10-26 | QIAGEN GmbH | Enrichment method |
| EP4077660A1 (en) * | 2019-12-16 | 2022-10-26 | QIAGEN GmbH | Method for enriching vesicular rna |
| EP4140568A4 (en) * | 2020-04-24 | 2024-05-22 | Toyobo Co., Ltd. | Ion exchange membrane |
| CN115698265A (zh) * | 2020-04-24 | 2023-02-03 | 高丽大学校算学协力团 | 微囊泡分离方法及微囊泡分离装置 |
| CA3180572A1 (en) | 2020-05-29 | 2021-12-02 | Johan Skog | Use of microvesicle signature for the diagnosis and treatment of kidney transplant rejection |
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| WO2022263500A1 (en) * | 2021-06-17 | 2022-12-22 | Qiagen Gmbh | Method for isolating non-vesicular mirna |
| US20250075258A1 (en) | 2021-07-16 | 2025-03-06 | Exosome Diagnostics, Inc. | Methods of detecting sjogren's syndrome using salivary exosomes |
| DE102021208893B3 (de) | 2021-08-13 | 2022-11-17 | Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. | Isolieren von Analyten unterschiedlicher Analyt-Klassen |
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| US20250163510A1 (en) | 2022-02-18 | 2025-05-22 | Exosome Diagnostics, Inc. | Use of microvesicle signatures in the identification and treatment of renal disorders |
| EP4584397A1 (en) | 2022-09-07 | 2025-07-16 | Exosome Diagnostics, Inc. | Methods of detecting sjögren's syndrome using salivary exosomes |
| CN115960885B (zh) * | 2022-10-09 | 2023-12-12 | 南京诺唯赞生物科技股份有限公司 | 一种提取肝素钠样品中核酸的方法及组合物 |
| KR102784335B1 (ko) * | 2023-02-09 | 2025-03-19 | 순천향대학교 산학협력단 | 세포유리핵산의 고농축화 분리 방법 |
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| US5438128A (en) * | 1992-02-07 | 1995-08-01 | Millipore Corporation | Method for rapid purifiction of nucleic acids using layered ion-exchange membranes |
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| ATE256735T1 (de) * | 1993-08-30 | 2004-01-15 | Promega Corp | Zusammensetzungen und verfahren zur reinigung von nukleinsäuren |
| FR2788780B1 (fr) * | 1999-01-27 | 2001-03-30 | Ap Cells Inc | Procede de preparation de vesicules membranaires |
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| KR20140005688A (ko) * | 2012-07-06 | 2014-01-15 | 삼성전자주식회사 | 사용자 인터페이스 방법 및 장치 |
| CN104995311A (zh) * | 2012-08-30 | 2015-10-21 | 外来体诊断公司 | 用于核酸测定的对照 |
| KR101933621B1 (ko) * | 2012-09-28 | 2018-12-28 | 삼성전자주식회사 | 소포를 분리하기 위한 조성물, 키트 및 이를 이용하여 소포를 분리하는 방법 |
| EP3499212B1 (en) | 2013-01-03 | 2020-08-12 | Exosome Diagnostics, Inc. | Methods for isolating microvesicles |
| SG11201700146QA (en) | 2014-07-09 | 2017-02-27 | Exosome Diagnostics Inc | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
-
2015
- 2015-07-09 SG SG11201700146QA patent/SG11201700146QA/en unknown
- 2015-07-09 EP EP19201765.5A patent/EP3623792B1/en active Active
- 2015-07-09 KR KR1020177003611A patent/KR102421185B1/ko active Active
- 2015-07-09 KR KR1020227023873A patent/KR102596577B1/ko active Active
- 2015-07-09 US US15/325,021 patent/US10465183B2/en active Active
- 2015-07-09 CN CN202110781733.3A patent/CN113699143B/zh active Active
- 2015-07-09 EP EP15818561.1A patent/EP3167062B1/en active Active
- 2015-07-09 CA CA2954576A patent/CA2954576C/en active Active
- 2015-07-09 WO PCT/US2015/039760 patent/WO2016007755A1/en not_active Ceased
- 2015-07-09 CN CN201580049375.XA patent/CN107002075B/zh active Active
- 2015-07-09 JP JP2017501235A patent/JP6759182B2/ja active Active
- 2015-07-09 AU AU2015287763A patent/AU2015287763B2/en active Active
- 2015-07-09 ES ES15818561T patent/ES2762677T3/es active Active
- 2015-07-09 CA CA3084920A patent/CA3084920C/en active Active
- 2015-07-09 ES ES19201765T patent/ES2898254T3/es active Active
-
2017
- 2017-01-09 IL IL250000A patent/IL250000B/en active IP Right Grant
-
2019
- 2019-10-17 AU AU2019250221A patent/AU2019250221B2/en active Active
-
2020
- 2020-09-02 JP JP2020147390A patent/JP7128866B2/ja active Active
-
2021
- 2021-01-20 IL IL280307A patent/IL280307B/en unknown
Also Published As
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| FGA | Letters patent sealed or granted (standard patent) |