KR101831331B1 - Ｃａｒ 효소들 및 지방족 알코올들의 개선된 생산 - Google Patents

Ｃａｒ 효소들 및 지방족 알코올들의 개선된 생산 Download PDF

Info

Publication number
KR101831331B1
KR101831331B1 KR1020147030704A KR20147030704A KR101831331B1 KR 101831331 B1 KR101831331 B1 KR 101831331B1 KR 1020147030704 A KR1020147030704 A KR 1020147030704A KR 20147030704 A KR20147030704 A KR 20147030704A KR 101831331 B1 KR101831331 B1 KR 101831331B1
Authority
KR
South Korea
Prior art keywords
ala
leu
val
gly
asp
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020147030704A
Other languages
English (en)
Korean (ko)
Other versions
KR20150032660A (ko
Inventor
데렉 엘. 그린필드
엘리자베스 제이. 클라크
엘리 에스. 그로반
비크란트 알라가다
성원 이
쉐지 리
지하오 흐
바오롱 주
Original Assignee
알이지 라이프 사이언시스, 엘엘씨
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 알이지 라이프 사이언시스, 엘엘씨 filed Critical 알이지 라이프 사이언시스, 엘엘씨
Publication of KR20150032660A publication Critical patent/KR20150032660A/ko
Application granted granted Critical
Publication of KR101831331B1 publication Critical patent/KR101831331B1/ko
Assigned to 게노마티카 인코포레이티드 reassignment 게노마티카 인코포레이티드 권리의 전부이전등록 Assignors: 알이지 라이프 사이언시스, 엘엘씨
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0105—Long-chain-fatty-acyl-CoA reductase (1.2.1.50)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/99—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with other acceptors (1.2.99)
    • C12Y102/99006—Carboxylate reductase (1.2.99.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01041—Beta-ketoacyl-acyl-carrier-protein synthase I (2.3.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020147030704A 2012-04-02 2013-04-02 Ｃａｒ 효소들 및 지방족 알코올들의 개선된 생산 Active KR101831331B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261619309P 2012-04-02 2012-04-02
US61/619,309 2012-04-02
PCT/US2013/035040 WO2013152052A2 (en) 2012-04-02 2013-04-02 Novel car enzymes and improved production of fatty alcohols

Related Child Applications (1)

Application Number Title Priority Date Filing Date
KR1020177026945A Division KR20170113710A (ko) 2012-04-02 2013-04-02 Ｃａｒ 효소들 및 지방족 알코올들의 개선된 생산

Publications (2)

Publication Number Publication Date
KR20150032660A KR20150032660A (ko) 2015-03-27
KR101831331B1 true KR101831331B1 (ko) 2018-02-23

Family

ID=49237582

Family Applications (6)

Application Number Title Priority Date Filing Date
KR1020147030704A Active KR101831331B1 (ko) 2012-04-02 2013-04-02 Ｃａｒ 효소들 및 지방족 알코올들의 개선된 생산
KR1020207009760A Expired - Fee Related KR102282677B1 (ko) 2012-04-02 2013-04-02 Ｃａｒ 효소들 및 지방족 알코올들의 개선된 생산
KR1020217023277A Ceased KR20210095226A (ko) 2012-04-02 2013-04-02 Ｃａｒ 효소들 및 지방족 알코올들의 개선된 생산
KR1020197017717A Expired - Fee Related KR102185762B1 (ko) 2012-04-02 2013-04-02 Ｃａｒ 효소들 및 지방족 알코올들의 개선된 생산
KR1020177026945A Ceased KR20170113710A (ko) 2012-04-02 2013-04-02 Ｃａｒ 효소들 및 지방족 알코올들의 개선된 생산
KR1020197005741A Expired - Fee Related KR102114841B1 (ko) 2012-04-02 2013-04-02 Ｃａｒ 효소들 및 지방족 알코올들의 개선된 생산

Family Applications After (5)

Application Number Title Priority Date Filing Date
KR1020207009760A Expired - Fee Related KR102282677B1 (ko) 2012-04-02 2013-04-02 Ｃａｒ 효소들 및 지방족 알코올들의 개선된 생산
KR1020217023277A Ceased KR20210095226A (ko) 2012-04-02 2013-04-02 Ｃａｒ 효소들 및 지방족 알코올들의 개선된 생산
KR1020197017717A Expired - Fee Related KR102185762B1 (ko) 2012-04-02 2013-04-02 Ｃａｒ 효소들 및 지방족 알코올들의 개선된 생산
KR1020177026945A Ceased KR20170113710A (ko) 2012-04-02 2013-04-02 Ｃａｒ 효소들 및 지방족 알코올들의 개선된 생산
KR1020197005741A Expired - Fee Related KR102114841B1 (ko) 2012-04-02 2013-04-02 Ｃａｒ 효소들 및 지방족 알코올들의 개선된 생산

Country Status (14)

Country Link
US (4) US9340801B2 (https=)
EP (6) EP4186974A1 (https=)
JP (1) JP6230594B2 (https=)
KR (6) KR101831331B1 (https=)
CN (1) CN104704113B (https=)
AU (2) AU2013243602B2 (https=)
BR (2) BR112014024674B1 (https=)
CA (1) CA2883064A1 (https=)
CO (1) CO7170148A2 (https=)
ES (3) ES2666157T3 (https=)
MX (2) MX343296B (https=)
MY (1) MY170889A (https=)
SI (1) SI3385374T1 (https=)
WO (1) WO2013152052A2 (https=)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101700296B1 (ko) * 2008-10-28 2017-01-26 알이지 라이프 사이언시스, 엘엘씨 지방족 알코올들을 생산하기 위한 방법들과 조성물들
CN104704113B (zh) * 2012-04-02 2018-01-23 Reg生命科学有限责任公司 Car酶及脂肪醇的改良生产
MX379251B (es) * 2012-04-02 2025-03-11 Genomatica Inc Producción mejorada de derivados de ácidos grasos.
SG11201501013PA (en) 2012-08-10 2015-04-29 Opx Biotechnologies Inc Microorganisms and methods for the production of fatty acids and fatty acid derived products
WO2014145332A1 (en) * 2013-03-15 2014-09-18 Opx Biotechnologies, Inc. Monfunctional mcr + 3-hp dehydrogenase
WO2014146026A1 (en) 2013-03-15 2014-09-18 Opx Biotechnologies, Inc. Bioproduction of chemicals
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
CN106795483A (zh) 2013-07-19 2017-05-31 嘉吉公司 用于生产脂肪酸和脂肪酸衍生产物的微生物及方法
KR20170029625A (ko) * 2014-07-18 2017-03-15 알이지 라이프 사이언시스, 엘엘씨 지방 디올의 미생물 생산
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
CN105985987B (zh) * 2015-03-02 2020-01-31 苏州安捷生物科技有限公司 一种生物法制备脂肪醇的方法
EP3430133A1 (en) 2016-03-17 2019-01-23 INVISTA Textiles (U.K.) Limited Carboxylic acid reductase polypeptides and variants having improved activity, materials and processes relating thereto
EP3577227A4 (en) 2017-02-02 2020-12-30 Cargill Inc. GENETICALLY MODIFIED CELLS PRODUCING C6-C10 FATTY ACID DERIVATIVES
CN111094558A (zh) 2017-04-03 2020-05-01 基因组股份公司 对产生中链脂肪酸衍生物具有改善的活性的硫酯酶变体
US12098168B2 (en) 2018-08-31 2024-09-24 Genomatica, Inc. XylR mutant for improved xylose utilization or improved co-utilization of glucose and xylose preliminary
WO2020047088A1 (en) 2018-08-31 2020-03-05 Genomatica, Inc. Production of non-native monounsaturated fatty acids in bacteria
KR20230160398A (ko) 2021-03-30 2023-11-23 아사히 가세이 가부시키가이샤 카르복실산 환원 활성을 갖는 재조합 폴리펩티드
WO2023178193A1 (en) 2022-03-16 2023-09-21 Genomatica, Inc. Acyl-acp thioesterase variants and uses thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100298612A1 (en) * 2009-05-22 2010-11-25 Codexis, Inc. Engineered biosynthesis of fatty alcohols

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5424202A (en) 1988-08-31 1995-06-13 The University Of Florida Ethanol production by recombinant hosts
US5482846A (en) 1988-08-31 1996-01-09 University Of Florida Ethanol production in Gram-positive microbes
US5028539A (en) 1988-08-31 1991-07-02 The University Of Florida Ethanol production using engineered mutant E. coli
US5000000A (en) 1988-08-31 1991-03-19 University Of Florida Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes
WO1991016427A1 (en) 1990-04-24 1991-10-31 Stratagene Methods for phenotype creation from multiple gene populations
US5602030A (en) 1994-03-28 1997-02-11 University Of Florida Research Foundation Recombinant glucose uptake system
US6428767B1 (en) 1995-05-12 2002-08-06 E. I. Du Pont De Nemours And Company Method for identifying the source of carbon in 1,3-propanediol
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US7056714B2 (en) * 2003-03-11 2006-06-06 University Of Iowa Research Foundation, Inc. Carboxylic acid reductase polypeptide, nucleotide sequence encoding same and methods of use
DE102004052115A1 (de) 2004-10-26 2006-04-27 Westfälische Wilhelms-Universität Münster Mikroorganismen zur Herstellung von Fettsäureestern, Verfahren zu deren Herstellung und deren Verwendung
EP2024491B1 (en) 2006-05-19 2014-11-19 LS9, Inc. Production of fatty acids and derivatives thereof
CA3178593A1 (en) 2007-03-28 2008-10-02 Genomatica, Inc. Enhanced production of fatty acid derivatives
JP5912251B2 (ja) 2007-05-22 2016-06-01 アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー 炭化水素産生遺伝子およびその使用方法
TWI351779B (en) 2007-12-03 2011-11-01 Advance Smart Ind Ltd Apparatus and method for correcting residual capac
US8183028B2 (en) 2007-12-21 2012-05-22 Ls9, Inc. Methods and compositions for producing olefins
JP2012504963A (ja) 2008-10-07 2012-03-01 エルエス９・インコーポレイテッド 脂肪アルデヒドを生産するための方法および組成物
KR101700296B1 (ko) 2008-10-28 2017-01-26 알이지 라이프 사이언시스, 엘엘씨 지방족 알코올들을 생산하기 위한 방법들과 조성물들
EP3564345A1 (en) * 2008-12-23 2019-11-06 REG Life Sciences, LLC Methods and compositions related to thioesterase enzymes
BRPI1009980A2 (pt) 2009-05-01 2015-08-25 Univ California Produtos de ésteres de ácidos graxos a partir de polímeros de biomassa
US8377666B2 (en) * 2009-10-13 2013-02-19 Genomatica, Inc. Microorganisms for the production of 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-coa, putrescine and related compounds, and methods related thereto
WO2011100667A1 (en) 2010-02-14 2011-08-18 Ls9, Inc. Surfactant and cleaning compositions comprising microbially produced branched fatty alcohols
US20110250663A1 (en) 2010-04-08 2011-10-13 Ls9, Inc. Methods and compositions related to fatty alcohol biosynthetic enzymes
EP3702363A1 (en) * 2011-04-01 2020-09-02 Genomatica, Inc. Fadr overexpressing bacterial cells for improved production of fatty acids and derivatives thereof
JP2014518613A (ja) * 2011-04-01 2014-08-07 ジェノマティカ・インコーポレイテッド メタクリル酸およびメタクリレートエステルの産生のための微生物ならびにそれに関連する方法
US8617862B2 (en) * 2011-06-22 2013-12-31 Genomatica, Inc. Microorganisms for producing propylene and methods related thereto
CN104704113B (zh) * 2012-04-02 2018-01-23 Reg生命科学有限责任公司 Car酶及脂肪醇的改良生产

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100298612A1 (en) * 2009-05-22 2010-11-25 Codexis, Inc. Engineered biosynthesis of fatty alcohols

Also Published As

Publication number Publication date
WO2013152052A2 (en) 2013-10-10
AU2013243602B2 (en) 2016-11-24
EP2834350A2 (en) 2015-02-11
AU2016231497B2 (en) 2017-08-31
EP3385374B1 (en) 2020-01-08
EP4186974A1 (en) 2023-05-31
MX358177B (es) 2018-08-08
KR102114841B1 (ko) 2020-05-26
EP3385374A1 (en) 2018-10-10
JP2015512645A (ja) 2015-04-30
US9758769B2 (en) 2017-09-12
KR20150032660A (ko) 2015-03-27
EP2834350B1 (en) 2016-11-16
EP3674399A1 (en) 2020-07-01
EP3153579A1 (en) 2017-04-12
US20150064757A1 (en) 2015-03-05
WO2013152052A3 (en) 2014-03-13
KR20210095226A (ko) 2021-07-30
BR112014024674A2 (pt) 2018-11-21
EP3674399B1 (en) 2021-09-22
ES2666157T3 (es) 2018-05-03
EP3985108A1 (en) 2022-04-20
KR102282677B1 (ko) 2021-07-28
ES2613553T3 (es) 2017-05-24
US20160326499A1 (en) 2016-11-10
KR20200038559A (ko) 2020-04-13
BR112014024674B1 (pt) 2022-06-07
US10301603B2 (en) 2019-05-28
AU2016231497A1 (en) 2016-10-06
CN104704113A (zh) 2015-06-10
KR20190022935A (ko) 2019-03-06
US9340801B2 (en) 2016-05-17
AU2013243602A1 (en) 2014-11-13
KR20190076059A (ko) 2019-07-01
EP3153579B1 (en) 2018-03-14
US11034937B2 (en) 2021-06-15
KR102185762B1 (ko) 2020-12-02
JP6230594B2 (ja) 2017-11-15
BR122019026987B1 (pt) 2022-01-18
US20200109374A1 (en) 2020-04-09
ES2786633T3 (es) 2020-10-13
SI3385374T1 (sl) 2020-09-30
CN104704113B (zh) 2018-01-23
US20180135028A1 (en) 2018-05-17
KR20170113710A (ko) 2017-10-12
CO7170148A2 (es) 2015-01-28
MX2014011903A (es) 2015-05-12
MX343296B (es) 2016-11-01
MY170889A (en) 2019-09-12
CA2883064A1 (en) 2013-10-10

Similar Documents

Publication Publication Date Title
KR101831331B1 (ko) Ｃａｒ 효소들 및 지방족 알코올들의 개선된 생산
KR102166271B1 (ko) 지방산 유도체들의 개선된 생산
KR102339617B1 (ko) 개선된 에스테르 신타아제 특성을 갖는 효소 변이체
KR102265148B1 (ko) 개선된 특성들을 갖는 아실-ａｃｐ 레덕타아제
KR20150094741A (ko) 지방산 유도체의 ａｃｐ­매개 생성
KR20160049016A (ko) 개선된 아세틸-ｃｏａ 카르복실라아제 변이체

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20141031

Patent event code: PA01051R01D

Comment text: International Patent Application

N231 Notification of change of applicant
PN2301 Change of applicant

Patent event date: 20150128

Comment text: Notification of Change of Applicant

Patent event code: PN23011R01D

PG1501 Laying open of application
N231 Notification of change of applicant
PN2301 Change of applicant

Patent event date: 20160226

Comment text: Notification of Change of Applicant

Patent event code: PN23011R01D

PN2301 Change of applicant

Patent event date: 20160714

Comment text: Notification of Change of Applicant

Patent event code: PN23011R01D

A201 Request for examination
A302 Request for accelerated examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20160810

Comment text: Request for Examination of Application

PA0302 Request for accelerated examination

Patent event date: 20160810

Patent event code: PA03022R01D

Comment text: Request for Accelerated Examination

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20161021

Patent event code: PE09021S01D

E90F Notification of reason for final refusal
PE0902 Notice of grounds for rejection

Comment text: Final Notice of Reason for Refusal

Patent event date: 20170623

Patent event code: PE09021S02D

A107 Divisional application of patent
PA0104 Divisional application for international application

Comment text: Divisional Application for International Patent

Patent event code: PA01041R01D

Patent event date: 20170922

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 20171116

PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 20180214

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 20180219

End annual number: 3

Start annual number: 1

PG1601 Publication of registration
PR1001 Payment of annual fee

Payment date: 20210115

Start annual number: 4

End annual number: 4

PR1001 Payment of annual fee

Payment date: 20220103

Start annual number: 5

End annual number: 5

PR1001 Payment of annual fee

Payment date: 20230213

Start annual number: 6

End annual number: 6

PR1001 Payment of annual fee

Payment date: 20240118

Start annual number: 7

End annual number: 7

PR1001 Payment of annual fee

Payment date: 20241230

Start annual number: 8

End annual number: 8