JP6230594B2 - Ｃａｒ酵素、および改良された脂肪アルコール産生方法 - Google Patents

Ｃａｒ酵素、および改良された脂肪アルコール産生方法 Download PDF

Info

Publication number
JP6230594B2
JP6230594B2 JP2015504688A JP2015504688A JP6230594B2 JP 6230594 B2 JP6230594 B2 JP 6230594B2 JP 2015504688 A JP2015504688 A JP 2015504688A JP 2015504688 A JP2015504688 A JP 2015504688A JP 6230594 B2 JP6230594 B2 JP 6230594B2
Authority
JP
Japan
Prior art keywords
polypeptide
mutant
fatty alcohol
host cell
fatty
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2015504688A
Other languages
English (en)
Japanese (ja)
Other versions
JP2015512645A (ja
JP2015512645A5 (https=
Inventor
デレク エル． グリーンフィールド
デレク エル． グリーンフィールド
エリザベス ジェイ． クラーク
エリザベス ジェイ． クラーク
イーライ エス． グローバン
イーライ エス． グローバン
ヴィクランス アルラガッダ
ヴィクランス アルラガッダ
スンウォン リー
スンウォン リー
シュエチ リ
シュエチ リ
チーハオ フ
チーハオ フ
バオロン チュ
バオロン チュ
Original Assignee
アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー
アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー, アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー filed Critical アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー
Publication of JP2015512645A publication Critical patent/JP2015512645A/ja
Publication of JP2015512645A5 publication Critical patent/JP2015512645A5/ja
Application granted granted Critical
Publication of JP6230594B2 publication Critical patent/JP6230594B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0105—Long-chain-fatty-acyl-CoA reductase (1.2.1.50)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/99—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with other acceptors (1.2.99)
    • C12Y102/99006—Carboxylate reductase (1.2.99.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01041—Beta-ketoacyl-acyl-carrier-protein synthase I (2.3.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2015504688A 2012-04-02 2013-04-02 Ｃａｒ酵素、および改良された脂肪アルコール産生方法 Active JP6230594B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261619309P 2012-04-02 2012-04-02
US61/619,309 2012-04-02
PCT/US2013/035040 WO2013152052A2 (en) 2012-04-02 2013-04-02 Novel car enzymes and improved production of fatty alcohols

Publications (3)

Publication Number Publication Date
JP2015512645A JP2015512645A (ja) 2015-04-30
JP2015512645A5 JP2015512645A5 (https=) 2016-09-29
JP6230594B2 true JP6230594B2 (ja) 2017-11-15

Family

ID=49237582

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2015504688A Active JP6230594B2 (ja) 2012-04-02 2013-04-02 Ｃａｒ酵素、および改良された脂肪アルコール産生方法

Country Status (14)

Country Link
US (4) US9340801B2 (https=)
EP (6) EP3153579B1 (https=)
JP (1) JP6230594B2 (https=)
KR (6) KR20210095226A (https=)
CN (1) CN104704113B (https=)
AU (2) AU2013243602B2 (https=)
BR (2) BR122019026987B1 (https=)
CA (1) CA2883064A1 (https=)
CO (1) CO7170148A2 (https=)
ES (3) ES2613553T3 (https=)
MX (2) MX358177B (https=)
MY (1) MY170889A (https=)
SI (1) SI3385374T1 (https=)
WO (1) WO2013152052A2 (https=)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101636403B1 (ko) * 2008-10-28 2016-07-05 알이지 라이프 사이언시스, 엘엘씨 지방족 알코올들을 생산하기 위한 방법들과 조성물들
EP2834352B1 (en) * 2012-04-02 2020-01-08 Genomatica, Inc. Improved production of fatty acid derivatives
BR122019026987B1 (pt) * 2012-04-02 2022-01-18 Genomatica, Inc Polipeptídeo variante da ácido carboxílico redutase (car), célula hospedeira recombinante, cultura de células e método de produzir uma composição de álcool graxo com alto título, rendimento ou produtividade
MX2015001780A (es) 2012-08-10 2015-09-28 Opx Biotechnologies Inc Microorganismos y métodos para la producción de ácidos grasos y productos derivados de ácidos grasos.
US20150119601A1 (en) 2013-03-15 2015-04-30 Opx Biotechnologies, Inc. Monofunctional mcr + 3-hp dehydrogenase
WO2014145344A2 (en) * 2013-03-15 2014-09-18 Opx Biotechnologies, Inc. Control of growth-induction-production phases
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
EP3022310B1 (en) 2013-07-19 2019-10-16 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
EP4089176A1 (en) * 2014-07-18 2022-11-16 Genomatica, Inc. Microbial production of 1,3 diols
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
CN105985987B (zh) * 2015-03-02 2020-01-31 苏州安捷生物科技有限公司 一种生物法制备脂肪醇的方法
US10538746B2 (en) 2016-03-17 2020-01-21 INVISTA North America S.à r.l. Polypeptides and variants having improved activity, materials and processes relating thereto
US11345938B2 (en) 2017-02-02 2022-05-31 Cargill, Incorporated Genetically modified cells that produce C6-C10 fatty acid derivatives
MY205555A (en) 2017-04-03 2024-10-25 Genomatica Inc Thioesterase variants having improved activity for the production of medium-chain fatty acid derivatives
EP3844179A4 (en) 2018-08-31 2022-08-31 Genomatica, Inc. XYLR MUTANT FOR IMPROVED XYLOSE UTILIZATION OR IMPROVED CO-UTILIZATION OF GLUCOSE AND XYLOSE
US12221644B2 (en) 2018-08-31 2025-02-11 Genomatica, Inc. Production of non-native monounsaturated fatty acids in bacteria
JPWO2022209763A1 (https=) 2021-03-30 2022-10-06
EP4493683A1 (en) 2022-03-16 2025-01-22 Genomatica, Inc. Acyl-acp thioesterase variants and uses thereof

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5028539A (en) 1988-08-31 1991-07-02 The University Of Florida Ethanol production using engineered mutant E. coli
US5000000A (en) 1988-08-31 1991-03-19 University Of Florida Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes
US5482846A (en) 1988-08-31 1996-01-09 University Of Florida Ethanol production in Gram-positive microbes
US5424202A (en) 1988-08-31 1995-06-13 The University Of Florida Ethanol production by recombinant hosts
WO1991016427A1 (en) 1990-04-24 1991-10-31 Stratagene Methods for phenotype creation from multiple gene populations
US5602030A (en) 1994-03-28 1997-02-11 University Of Florida Research Foundation Recombinant glucose uptake system
US6428767B1 (en) 1995-05-12 2002-08-06 E. I. Du Pont De Nemours And Company Method for identifying the source of carbon in 1,3-propanediol
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US7056714B2 (en) * 2003-03-11 2006-06-06 University Of Iowa Research Foundation, Inc. Carboxylic acid reductase polypeptide, nucleotide sequence encoding same and methods of use
DE102004052115A1 (de) 2004-10-26 2006-04-27 Westfälische Wilhelms-Universität Münster Mikroorganismen zur Herstellung von Fettsäureestern, Verfahren zu deren Herstellung und deren Verwendung
CA2650773C (en) 2006-05-19 2014-12-02 Jay D. Keasling Microorganisms transformed with acetyl-coa carboxylase and wax synthase for production of fatty acid derivatives
CN101680009B (zh) 2007-03-28 2017-02-22 Reg生命科学有限责任公司 提高脂肪酸衍生物的产量
BRPI0813359A2 (pt) 2007-05-22 2014-10-14 Ls9 Inc Ácidos nucleicos isolados, vetor, células, métodos para produzir um hidrocarboneto, para produzir uma cetona alifática, para produzir um polipeptídeo purificado, para produzir um hidrocarboneto, para produzir biocombustíveis, hidrocarboneto, biocombustível, polipeptídeo isolado, ácido nucleico isolado, organismos geneticamente engenheirados, método para identificar uma enzima útil para a produção de hidrocarbonetos
TWI351779B (en) 2007-12-03 2011-11-01 Advance Smart Ind Ltd Apparatus and method for correcting residual capac
WO2009085278A1 (en) 2007-12-21 2009-07-09 Ls9, Inc. Methods and compositions for producing olefins
KR101717569B1 (ko) 2008-10-07 2017-03-17 알이지 라이프 사이언시스, 엘엘씨 지방족 알데히드를 생산하기 위한 방법과 조성물들
KR101636403B1 (ko) * 2008-10-28 2016-07-05 알이지 라이프 사이언시스, 엘엘씨 지방족 알코올들을 생산하기 위한 방법들과 조성물들
CA3055144C (en) 2008-12-23 2022-07-19 REG Life Sciences, LLC Methods and compositions related to thioesterase enzymes
US20120115195A1 (en) 2009-05-01 2012-05-10 The Regents Of The University Of California Product of fatty acid esters from biomass polymers
BRPI1010920B1 (pt) * 2009-05-22 2020-04-28 Codexis Inc processo para a produção biológica de álcoois graxos
KR20120083908A (ko) * 2009-10-13 2012-07-26 게노마티카 인코포레이티드 1,4-부탄다이올, 4-하이드록시부탄알, 4-하이드록시부티릴-ｃｏａ, 푸트레신 및 관련 화합물의 제조를 위한 미생물 및 관련 방법
WO2011100667A1 (en) 2010-02-14 2011-08-18 Ls9, Inc. Surfactant and cleaning compositions comprising microbially produced branched fatty alcohols
US20110250663A1 (en) 2010-04-08 2011-10-13 Ls9, Inc. Methods and compositions related to fatty alcohol biosynthetic enzymes
BR112013025226A2 (pt) * 2011-04-01 2018-09-11 Genomatica Inc micro-organismos para produzir ácido metacrílico e ésteres de metacrilato e métodos relacionados a eles
WO2012154329A1 (en) * 2011-04-01 2012-11-15 Ls9, Inc. Methods and compositions for improved production of fatty acids and derivatives thereof
US8617862B2 (en) * 2011-06-22 2013-12-31 Genomatica, Inc. Microorganisms for producing propylene and methods related thereto
BR122019026987B1 (pt) * 2012-04-02 2022-01-18 Genomatica, Inc Polipeptídeo variante da ácido carboxílico redutase (car), célula hospedeira recombinante, cultura de células e método de produzir uma composição de álcool graxo com alto título, rendimento ou produtividade

Also Published As

Publication number Publication date
US10301603B2 (en) 2019-05-28
MY170889A (en) 2019-09-12
KR20170113710A (ko) 2017-10-12
US11034937B2 (en) 2021-06-15
MX2014011903A (es) 2015-05-12
EP3153579B1 (en) 2018-03-14
KR20150032660A (ko) 2015-03-27
ES2613553T3 (es) 2017-05-24
EP2834350B1 (en) 2016-11-16
MX358177B (es) 2018-08-08
EP3385374A1 (en) 2018-10-10
KR102185762B1 (ko) 2020-12-02
US9340801B2 (en) 2016-05-17
US20180135028A1 (en) 2018-05-17
US20200109374A1 (en) 2020-04-09
WO2013152052A2 (en) 2013-10-10
CO7170148A2 (es) 2015-01-28
CN104704113B (zh) 2018-01-23
KR20200038559A (ko) 2020-04-13
AU2013243602B2 (en) 2016-11-24
US20160326499A1 (en) 2016-11-10
ES2786633T3 (es) 2020-10-13
CA2883064A1 (en) 2013-10-10
EP3385374B1 (en) 2020-01-08
SI3385374T1 (sl) 2020-09-30
AU2016231497A1 (en) 2016-10-06
AU2016231497B2 (en) 2017-08-31
US20150064757A1 (en) 2015-03-05
BR122019026987B1 (pt) 2022-01-18
KR102114841B1 (ko) 2020-05-26
EP4186974A1 (en) 2023-05-31
EP3985108A1 (en) 2022-04-20
AU2013243602A1 (en) 2014-11-13
MX343296B (es) 2016-11-01
KR20210095226A (ko) 2021-07-30
KR102282677B1 (ko) 2021-07-28
JP2015512645A (ja) 2015-04-30
BR112014024674B1 (pt) 2022-06-07
WO2013152052A3 (en) 2014-03-13
KR20190076059A (ko) 2019-07-01
EP3674399B1 (en) 2021-09-22
ES2666157T3 (es) 2018-05-03
CN104704113A (zh) 2015-06-10
EP3153579A1 (en) 2017-04-12
KR101831331B1 (ko) 2018-02-23
KR20190022935A (ko) 2019-03-06
EP3674399A1 (en) 2020-07-01
US9758769B2 (en) 2017-09-12
BR112014024674A2 (pt) 2018-11-21
EP2834350A2 (en) 2015-02-11

Similar Documents

Publication Publication Date Title
JP6230594B2 (ja) Ｃａｒ酵素、および改良された脂肪アルコール産生方法
JP6738852B2 (ja) 改良された脂肪酸誘導体産生方法
JP7094343B2 (ja) 改良された特性を有するアシル－ａｃｐレダクターゼ
JP2016500261A (ja) 脂肪酸誘導体のａｃｐ媒介性生産方法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150108

A711 Notification of change in applicant

Free format text: JAPANESE INTERMEDIATE CODE: A711

Effective date: 20150209

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20150209

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20160224

A711 Notification of change in applicant

Free format text: JAPANESE INTERMEDIATE CODE: A711

Effective date: 20160523

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20160523

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160805

A871 Explanation of circumstances concerning accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A871

Effective date: 20160805

A975 Report on accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A971005

Effective date: 20160817

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20160829

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20161128

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20161226

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20170213

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20170512

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170809

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20170927

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20171017

R150 Certificate of patent or registration of utility model

Ref document number: 6230594

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

S111 Request for change of ownership or part of ownership

Free format text: JAPANESE INTERMEDIATE CODE: R313113

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250