AU2013243602B2 - Car enzymes and improved production of fatty alcohols - Google Patents

Car enzymes and improved production of fatty alcohols Download PDF

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Publication number
AU2013243602B2
AU2013243602B2 AU2013243602A AU2013243602A AU2013243602B2 AU 2013243602 B2 AU2013243602 B2 AU 2013243602B2 AU 2013243602 A AU2013243602 A AU 2013243602A AU 2013243602 A AU2013243602 A AU 2013243602A AU 2013243602 B2 AU2013243602 B2 AU 2013243602B2
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Prior art keywords
mutations
fatty
host cell
fatty alcohol
polypeptide
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AU2013243602A
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AU2013243602A1 (en
Inventor
Vikranth Arlagadda
Elizabeth J. CLARKE
Derek L. Greenfield
Eli S. GROBAN
Zhihao Hu
Sungwon Lee
Xuezhi LI
Baolong Zhu
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Genomatica Inc
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Genomatica Inc
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Assigned to REG Life Sciences, LLC reassignment REG Life Sciences, LLC Request for Assignment Assignors: ARLAGADDA, VIKRANTH, CLARKE, ELIZABETH, GREENFIELD, DEREK, GROBAN, ELI, HU, ZHIHAO, LEE, SUNGWON, LI, Xuezhi, REG Life Sciences, LLC, ZHU, BAOLONG
Priority to AU2016231497A priority Critical patent/AU2016231497B2/en
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Publication of AU2013243602B2 publication Critical patent/AU2013243602B2/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0008Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1025Acyltransferases (2.3)
    • C12N9/1029Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y102/00Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0105Long-chain-fatty-acyl-CoA reductase (1.2.1.50)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y102/00Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/99Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with other acceptors (1.2.99)
    • C12Y102/99006Carboxylate reductase (1.2.99.6)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y203/00Acyltransferases (2.3)
    • C12Y203/01Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01041Beta-ketoacyl-acyl-carrier-protein synthase I (2.3.1.41)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/02Thioester hydrolases (3.1.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2203/00Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409Fatty acids
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/50Improvements relating to the production of bulk chemicals
    • Y02P20/52Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2013243602A 2012-04-02 2013-04-02 Car enzymes and improved production of fatty alcohols Ceased AU2013243602B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2016231497A AU2016231497B2 (en) 2012-04-02 2016-09-20 Novel car enzymes and improved production of fatty alcohols

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261619309P 2012-04-02 2012-04-02
US61/619,309 2012-04-02
PCT/US2013/035040 WO2013152052A2 (en) 2012-04-02 2013-04-02 Novel car enzymes and improved production of fatty alcohols

Related Child Applications (1)

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AU2016231497A Division AU2016231497B2 (en) 2012-04-02 2016-09-20 Novel car enzymes and improved production of fatty alcohols

Publications (2)

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AU2013243602A1 AU2013243602A1 (en) 2014-11-13
AU2013243602B2 true AU2013243602B2 (en) 2016-11-24

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AU2013243602A Ceased AU2013243602B2 (en) 2012-04-02 2013-04-02 Car enzymes and improved production of fatty alcohols
AU2016231497A Ceased AU2016231497B2 (en) 2012-04-02 2016-09-20 Novel car enzymes and improved production of fatty alcohols

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Country Status (14)

Country Link
US (4) US9340801B2 (https=)
EP (6) EP3153579B1 (https=)
JP (1) JP6230594B2 (https=)
KR (6) KR20210095226A (https=)
CN (1) CN104704113B (https=)
AU (2) AU2013243602B2 (https=)
BR (2) BR122019026987B1 (https=)
CA (1) CA2883064A1 (https=)
CO (1) CO7170148A2 (https=)
ES (3) ES2613553T3 (https=)
MX (2) MX358177B (https=)
MY (1) MY170889A (https=)
SI (1) SI3385374T1 (https=)
WO (1) WO2013152052A2 (https=)

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EP2834352B1 (en) * 2012-04-02 2020-01-08 Genomatica, Inc. Improved production of fatty acid derivatives
BR122019026987B1 (pt) * 2012-04-02 2022-01-18 Genomatica, Inc Polipeptídeo variante da ácido carboxílico redutase (car), célula hospedeira recombinante, cultura de células e método de produzir uma composição de álcool graxo com alto título, rendimento ou produtividade
MX2015001780A (es) 2012-08-10 2015-09-28 Opx Biotechnologies Inc Microorganismos y métodos para la producción de ácidos grasos y productos derivados de ácidos grasos.
US20150119601A1 (en) 2013-03-15 2015-04-30 Opx Biotechnologies, Inc. Monofunctional mcr + 3-hp dehydrogenase
WO2014145344A2 (en) * 2013-03-15 2014-09-18 Opx Biotechnologies, Inc. Control of growth-induction-production phases
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
EP3022310B1 (en) 2013-07-19 2019-10-16 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
EP4089176A1 (en) * 2014-07-18 2022-11-16 Genomatica, Inc. Microbial production of 1,3 diols
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
CN105985987B (zh) * 2015-03-02 2020-01-31 苏州安捷生物科技有限公司 一种生物法制备脂肪醇的方法
US10538746B2 (en) 2016-03-17 2020-01-21 INVISTA North America S.à r.l. Polypeptides and variants having improved activity, materials and processes relating thereto
US11345938B2 (en) 2017-02-02 2022-05-31 Cargill, Incorporated Genetically modified cells that produce C6-C10 fatty acid derivatives
MY205555A (en) 2017-04-03 2024-10-25 Genomatica Inc Thioesterase variants having improved activity for the production of medium-chain fatty acid derivatives
EP3844179A4 (en) 2018-08-31 2022-08-31 Genomatica, Inc. XYLR MUTANT FOR IMPROVED XYLOSE UTILIZATION OR IMPROVED CO-UTILIZATION OF GLUCOSE AND XYLOSE
US12221644B2 (en) 2018-08-31 2025-02-11 Genomatica, Inc. Production of non-native monounsaturated fatty acids in bacteria
JPWO2022209763A1 (https=) 2021-03-30 2022-10-06
EP4493683A1 (en) 2022-03-16 2025-01-22 Genomatica, Inc. Acyl-acp thioesterase variants and uses thereof

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Also Published As

Publication number Publication date
US10301603B2 (en) 2019-05-28
MY170889A (en) 2019-09-12
KR20170113710A (ko) 2017-10-12
US11034937B2 (en) 2021-06-15
MX2014011903A (es) 2015-05-12
EP3153579B1 (en) 2018-03-14
KR20150032660A (ko) 2015-03-27
ES2613553T3 (es) 2017-05-24
EP2834350B1 (en) 2016-11-16
MX358177B (es) 2018-08-08
EP3385374A1 (en) 2018-10-10
KR102185762B1 (ko) 2020-12-02
US9340801B2 (en) 2016-05-17
US20180135028A1 (en) 2018-05-17
US20200109374A1 (en) 2020-04-09
WO2013152052A2 (en) 2013-10-10
CO7170148A2 (es) 2015-01-28
CN104704113B (zh) 2018-01-23
KR20200038559A (ko) 2020-04-13
US20160326499A1 (en) 2016-11-10
ES2786633T3 (es) 2020-10-13
CA2883064A1 (en) 2013-10-10
EP3385374B1 (en) 2020-01-08
SI3385374T1 (sl) 2020-09-30
AU2016231497A1 (en) 2016-10-06
AU2016231497B2 (en) 2017-08-31
US20150064757A1 (en) 2015-03-05
BR122019026987B1 (pt) 2022-01-18
KR102114841B1 (ko) 2020-05-26
JP6230594B2 (ja) 2017-11-15
EP4186974A1 (en) 2023-05-31
EP3985108A1 (en) 2022-04-20
AU2013243602A1 (en) 2014-11-13
MX343296B (es) 2016-11-01
KR20210095226A (ko) 2021-07-30
KR102282677B1 (ko) 2021-07-28
JP2015512645A (ja) 2015-04-30
BR112014024674B1 (pt) 2022-06-07
WO2013152052A3 (en) 2014-03-13
KR20190076059A (ko) 2019-07-01
EP3674399B1 (en) 2021-09-22
ES2666157T3 (es) 2018-05-03
CN104704113A (zh) 2015-06-10
EP3153579A1 (en) 2017-04-12
KR101831331B1 (ko) 2018-02-23
KR20190022935A (ko) 2019-03-06
EP3674399A1 (en) 2020-07-01
US9758769B2 (en) 2017-09-12
BR112014024674A2 (pt) 2018-11-21
EP2834350A2 (en) 2015-02-11

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