ES2666157T3 - Enzimas CAR y producción mejorada de alcoholes grasos - Google Patents
Enzimas CAR y producción mejorada de alcoholes grasos Download PDFInfo
- Publication number
- ES2666157T3 ES2666157T3 ES16198810.0T ES16198810T ES2666157T3 ES 2666157 T3 ES2666157 T3 ES 2666157T3 ES 16198810 T ES16198810 T ES 16198810T ES 2666157 T3 ES2666157 T3 ES 2666157T3
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- host cell
- recombinant host
- fatty
- fatty alcohol
- polypeptide
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- C12N9/0004—Oxidoreductases (1.)
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- C—CHEMISTRY; METALLURGY
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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- C—CHEMISTRY; METALLURGY
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- C—CHEMISTRY; METALLURGY
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
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- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
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- C—CHEMISTRY; METALLURGY
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- C12Y—ENZYMES
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- C—CHEMISTRY; METALLURGY
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- C12Y—ENZYMES
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- C12Y—ENZYMES
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
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- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
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| KR101700296B1 (ko) * | 2008-10-28 | 2017-01-26 | 알이지 라이프 사이언시스, 엘엘씨 | 지방족 알코올들을 생산하기 위한 방법들과 조성물들 |
| CN104704113B (zh) * | 2012-04-02 | 2018-01-23 | Reg生命科学有限责任公司 | Car酶及脂肪醇的改良生产 |
| MX379251B (es) * | 2012-04-02 | 2025-03-11 | Genomatica Inc | Producción mejorada de derivados de ácidos grasos. |
| SG11201501013PA (en) | 2012-08-10 | 2015-04-29 | Opx Biotechnologies Inc | Microorganisms and methods for the production of fatty acids and fatty acid derived products |
| WO2014145332A1 (en) * | 2013-03-15 | 2014-09-18 | Opx Biotechnologies, Inc. | Monfunctional mcr + 3-hp dehydrogenase |
| WO2014146026A1 (en) | 2013-03-15 | 2014-09-18 | Opx Biotechnologies, Inc. | Bioproduction of chemicals |
| US11408013B2 (en) | 2013-07-19 | 2022-08-09 | Cargill, Incorporated | Microorganisms and methods for the production of fatty acids and fatty acid derived products |
| CN106795483A (zh) | 2013-07-19 | 2017-05-31 | 嘉吉公司 | 用于生产脂肪酸和脂肪酸衍生产物的微生物及方法 |
| KR20170029625A (ko) * | 2014-07-18 | 2017-03-15 | 알이지 라이프 사이언시스, 엘엘씨 | 지방 디올의 미생물 생산 |
| EP2993228B1 (en) | 2014-09-02 | 2019-10-09 | Cargill, Incorporated | Production of fatty acid esters |
| CN105985987B (zh) * | 2015-03-02 | 2020-01-31 | 苏州安捷生物科技有限公司 | 一种生物法制备脂肪醇的方法 |
| EP3430133A1 (en) | 2016-03-17 | 2019-01-23 | INVISTA Textiles (U.K.) Limited | Carboxylic acid reductase polypeptides and variants having improved activity, materials and processes relating thereto |
| EP3577227A4 (en) | 2017-02-02 | 2020-12-30 | Cargill Inc. | GENETICALLY MODIFIED CELLS PRODUCING C6-C10 FATTY ACID DERIVATIVES |
| CN111094558A (zh) | 2017-04-03 | 2020-05-01 | 基因组股份公司 | 对产生中链脂肪酸衍生物具有改善的活性的硫酯酶变体 |
| US12098168B2 (en) | 2018-08-31 | 2024-09-24 | Genomatica, Inc. | XylR mutant for improved xylose utilization or improved co-utilization of glucose and xylose preliminary |
| WO2020047088A1 (en) | 2018-08-31 | 2020-03-05 | Genomatica, Inc. | Production of non-native monounsaturated fatty acids in bacteria |
| KR20230160398A (ko) | 2021-03-30 | 2023-11-23 | 아사히 가세이 가부시키가이샤 | 카르복실산 환원 활성을 갖는 재조합 폴리펩티드 |
| WO2023178193A1 (en) | 2022-03-16 | 2023-09-21 | Genomatica, Inc. | Acyl-acp thioesterase variants and uses thereof |
Family Cites Families (28)
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| US5424202A (en) | 1988-08-31 | 1995-06-13 | The University Of Florida | Ethanol production by recombinant hosts |
| US5482846A (en) | 1988-08-31 | 1996-01-09 | University Of Florida | Ethanol production in Gram-positive microbes |
| US5028539A (en) | 1988-08-31 | 1991-07-02 | The University Of Florida | Ethanol production using engineered mutant E. coli |
| US5000000A (en) | 1988-08-31 | 1991-03-19 | University Of Florida | Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes |
| WO1991016427A1 (en) | 1990-04-24 | 1991-10-31 | Stratagene | Methods for phenotype creation from multiple gene populations |
| US5602030A (en) | 1994-03-28 | 1997-02-11 | University Of Florida Research Foundation | Recombinant glucose uptake system |
| US6428767B1 (en) | 1995-05-12 | 2002-08-06 | E. I. Du Pont De Nemours And Company | Method for identifying the source of carbon in 1,3-propanediol |
| US5965408A (en) | 1996-07-09 | 1999-10-12 | Diversa Corporation | Method of DNA reassembly by interrupting synthesis |
| US5939250A (en) | 1995-12-07 | 1999-08-17 | Diversa Corporation | Production of enzymes having desired activities by mutagenesis |
| US7056714B2 (en) * | 2003-03-11 | 2006-06-06 | University Of Iowa Research Foundation, Inc. | Carboxylic acid reductase polypeptide, nucleotide sequence encoding same and methods of use |
| DE102004052115A1 (de) | 2004-10-26 | 2006-04-27 | Westfälische Wilhelms-Universität Münster | Mikroorganismen zur Herstellung von Fettsäureestern, Verfahren zu deren Herstellung und deren Verwendung |
| EP2024491B1 (en) | 2006-05-19 | 2014-11-19 | LS9, Inc. | Production of fatty acids and derivatives thereof |
| CA3178593A1 (en) | 2007-03-28 | 2008-10-02 | Genomatica, Inc. | Enhanced production of fatty acid derivatives |
| JP5912251B2 (ja) | 2007-05-22 | 2016-06-01 | アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー | 炭化水素産生遺伝子およびその使用方法 |
| TWI351779B (en) | 2007-12-03 | 2011-11-01 | Advance Smart Ind Ltd | Apparatus and method for correcting residual capac |
| US8183028B2 (en) | 2007-12-21 | 2012-05-22 | Ls9, Inc. | Methods and compositions for producing olefins |
| JP2012504963A (ja) | 2008-10-07 | 2012-03-01 | エルエス９・インコーポレイテッド | 脂肪アルデヒドを生産するための方法および組成物 |
| KR101700296B1 (ko) | 2008-10-28 | 2017-01-26 | 알이지 라이프 사이언시스, 엘엘씨 | 지방족 알코올들을 생산하기 위한 방법들과 조성물들 |
| EP3564345A1 (en) * | 2008-12-23 | 2019-11-06 | REG Life Sciences, LLC | Methods and compositions related to thioesterase enzymes |
| BRPI1009980A2 (pt) | 2009-05-01 | 2015-08-25 | Univ California | Produtos de ésteres de ácidos graxos a partir de polímeros de biomassa |
| EP2432890B1 (en) * | 2009-05-22 | 2015-09-02 | Codexis, Inc. | Engineered biosynthesis of fatty alcohols |
| US8377666B2 (en) * | 2009-10-13 | 2013-02-19 | Genomatica, Inc. | Microorganisms for the production of 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-coa, putrescine and related compounds, and methods related thereto |
| WO2011100667A1 (en) | 2010-02-14 | 2011-08-18 | Ls9, Inc. | Surfactant and cleaning compositions comprising microbially produced branched fatty alcohols |
| US20110250663A1 (en) | 2010-04-08 | 2011-10-13 | Ls9, Inc. | Methods and compositions related to fatty alcohol biosynthetic enzymes |
| EP3702363A1 (en) * | 2011-04-01 | 2020-09-02 | Genomatica, Inc. | Fadr overexpressing bacterial cells for improved production of fatty acids and derivatives thereof |
| JP2014518613A (ja) * | 2011-04-01 | 2014-08-07 | ジェノマティカ・インコーポレイテッド | メタクリル酸およびメタクリレートエステルの産生のための微生物ならびにそれに関連する方法 |
| US8617862B2 (en) * | 2011-06-22 | 2013-12-31 | Genomatica, Inc. | Microorganisms for producing propylene and methods related thereto |
| CN104704113B (zh) * | 2012-04-02 | 2018-01-23 | Reg生命科学有限责任公司 | Car酶及脂肪醇的改良生产 |
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2016
- 2016-05-13 US US15/154,636 patent/US9758769B2/en active Active
- 2016-09-20 AU AU2016231497A patent/AU2016231497B2/en not_active Ceased
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2017
- 2017-09-05 US US15/695,205 patent/US10301603B2/en active Active
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2019
- 2019-05-16 US US16/414,550 patent/US11034937B2/en active Active
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