KR101791597B1 - 모그로사이드 화합물을 효소에 의해 합성하기 위한 방법 및 재료 - Google Patents

모그로사이드 화합물을 효소에 의해 합성하기 위한 방법 및 재료 Download PDF

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KR101791597B1
KR101791597B1 KR1020147016910A KR20147016910A KR101791597B1 KR 101791597 B1 KR101791597 B1 KR 101791597B1 KR 1020147016910 A KR1020147016910 A KR 1020147016910A KR 20147016910 A KR20147016910 A KR 20147016910A KR 101791597 B1 KR101791597 B1 KR 101791597B1
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야오콴 리우
정 엽 이
모니카 카레
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에볼바 에스아
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    • C12P33/00—Preparation of steroids
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids

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KR1020147016910A 2011-11-23 2012-11-19 모그로사이드 화합물을 효소에 의해 합성하기 위한 방법 및 재료 Active KR101791597B1 (ko)

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US201161563303P 2011-11-23 2011-11-23
US61/563,303 2011-11-23
PCT/IB2012/002857 WO2013076577A1 (en) 2011-11-23 2012-11-19 Methods and materials for enzymatic synthesis of mogroside compounds

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210050044A (ko) * 2019-10-25 2021-05-07 고려대학교 산학협력단 테르펜 당쇄 부가 유도체, 이의 제조방법 및 이를 유효성분으로 함유하는 조성물
US12234464B2 (en) 2018-11-09 2025-02-25 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ604915A (en) 2010-06-02 2014-10-31 Evolva Nutrition Inc Recombinant production of steviol glycosides
SG10201606567TA (en) 2011-08-08 2016-10-28 Evolva Sa Recombinant production of steviol glycosides
IN2014CN03557A (cg-RX-API-DMAC7.html) 2011-11-23 2015-10-09 Evolva Sa
TW201402599A (zh) * 2012-05-30 2014-01-16 Suntory Holdings Ltd 甜菊糖苷糖苷轉化酵素及編碼該酵素之基因
WO2014086842A1 (en) * 2012-12-04 2014-06-12 Evolva Sa Methods and materials for biosynthesis of mogroside compounds
CN105189771B (zh) 2013-02-11 2019-11-05 埃沃尔瓦公司 在重组宿主中有效产生甜菊醇糖苷
BR112017002783A2 (pt) 2014-08-11 2017-12-19 Evolva Sa produção de glicosídeos de esteviol em hospedeiros recombinantes
MY191735A (en) 2014-09-09 2022-07-13 Evolva Sa Production of steviol glycosides in recombinant hosts
CA2972739A1 (en) * 2014-09-11 2016-03-17 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) Methods of producing mogrosides and compositions comprising same and uses thereof
CA2963300C (en) 2014-10-01 2024-04-30 Evolva Sa Methods and materials for biosynthesis of mogroside compounds
JP6698028B2 (ja) 2014-10-17 2020-05-27 サントリーホールディングス株式会社 モグロール配糖体化酵素およびそれをコードする遺伝子
JP6850130B2 (ja) 2015-01-20 2021-03-31 サントリーホールディングス株式会社 モグロシドの調製方法
JP2018503388A (ja) 2015-01-30 2018-02-08 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． 組み換え宿主におけるステビオールグリコシドの生産
CN115851470B (zh) 2015-03-16 2025-09-02 帝斯曼知识产权资产管理有限公司 Udp-糖基转移酶
AU2016307066A1 (en) 2015-08-07 2018-02-08 Evolva Sa Production of steviol glycosides in recombinant hosts
CN108473528A (zh) 2015-10-29 2018-08-31 赛诺米克斯公司 高强度甜味剂
CN106924274B (zh) * 2015-12-29 2019-01-04 成都普睿法药物研发有限公司 葫芦烷型四环三萜类化合物抗肺纤维化应用
SG11201808856TA (en) 2016-04-13 2018-11-29 Evolva Sa Production of steviol glycosides in recombinant hosts
AU2017267214A1 (en) * 2016-05-16 2018-11-15 Evolva Sa Production of steviol glycosides in recombinant hosts
WO2017198682A1 (en) 2016-05-16 2017-11-23 Evolva Sa Production of steviol glycosides in recombinant hosts
CN109477126B (zh) * 2016-07-19 2023-06-20 三得利控股株式会社 罗汉果醇或罗汉果醇糖苷的生产方法
EP3535406A1 (en) 2016-11-07 2019-09-11 Evolva SA Production of steviol glycosides in recombinant hosts
WO2018204483A2 (en) 2017-05-03 2018-11-08 Senomyx, Inc. Methods for making high intensity sweeteners
MX2019013696A (es) 2017-05-19 2020-01-27 Coca Cola Co Composiciones edulcoradas de siamenósido i y usos de las mismas.
CA3064334A1 (en) 2017-06-02 2018-12-06 Givaudan Sa Compositions
WO2021214008A1 (en) 2020-04-20 2021-10-28 Givaudan Sa Compositions
EP3638804A1 (en) 2017-06-15 2020-04-22 Evolva SA Production of mogroside compounds in recombinant hosts
CN107189976B (zh) * 2017-07-28 2022-03-11 李华政 促进罗汉果ugt72b21基因表达的方法
KR20210089717A (ko) 2018-11-07 2021-07-16 피르메니히 인코포레이티드 고강도 감미료의 제조 방법
WO2020096905A1 (en) 2018-11-07 2020-05-14 Firmenich Incorporated Methods for making high intensity sweeteners
CN110669094B (zh) * 2019-10-14 2021-12-21 上海交通大学 罗汉果新苷及其制备方法
BR112022018627A2 (pt) * 2020-03-17 2022-12-20 Coca Cola Co Sistemas e métodos inovadores de produção de mogrosídeo
GB202007984D0 (en) 2020-05-28 2020-07-15 Givaudan Sa Compositions
CN112063647B (zh) * 2020-09-17 2023-05-02 云南农业大学 酿酒酵母重组菌Cuol01的构建方法、酿酒酵母重组菌Cuol02及应用
CN116064712B (zh) * 2022-10-08 2025-05-06 中国药科大学 一种罗汉果醇的生物合成方法
CN121127603A (zh) 2023-04-20 2025-12-12 帝斯曼知识产权资产管理有限公司 用于产生罗汉果苷的菌株和方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008065370A2 (en) 2006-12-01 2008-06-05 The University Of York Sesquiterpenoid modifying enzymes

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6399321B1 (en) * 1999-08-18 2002-06-04 National Research Council Of Canada Methods for screening UDP-glucose:glycoprotein glucosyltransferase (UGGT) activity and nucleic acid encoding for UGGT
EP1510573A1 (en) * 2003-09-01 2005-03-02 Austria Wirtschaftsservice Gesellschaft mit beschränkter Haftung Method for detoxification of mycotoxins
US20080148432A1 (en) * 2005-12-21 2008-06-19 Mark Scott Abad Transgenic plants with enhanced agronomic traits
EP1871883A1 (en) 2005-03-02 2008-01-02 Metanomics GmbH Process for the production of fine chemicals
US8257948B1 (en) * 2011-02-17 2012-09-04 Purecircle Usa Method of preparing alpha-glucosyl Stevia composition
US8956677B2 (en) 2005-11-23 2015-02-17 The Coca-Cola Company High-potency sweetener composition with glucosamine and compositions sweetened therewith
RU2455854C2 (ru) 2005-11-23 2012-07-20 Дзе Кока-Кола Компани Композиция интенсивного подсластителя с глюкозамином и подслащенные ею композиции
US7927851B2 (en) 2006-03-21 2011-04-19 Vineland Research And Innovation Centre Compositions having ent-kaurenoic acid 13-hydroxylase activity and methods for producing same
US20100143975A1 (en) * 2006-11-22 2010-06-10 The University Of York Monoterpenoid modifying enzymes
GB0904639D0 (en) * 2009-03-18 2009-04-29 Univ York Assay
NZ604915A (en) 2010-06-02 2014-10-31 Evolva Nutrition Inc Recombinant production of steviol glycosides
IN2014CN03557A (cg-RX-API-DMAC7.html) 2011-11-23 2015-10-09 Evolva Sa
WO2014086842A1 (en) 2012-12-04 2014-06-12 Evolva Sa Methods and materials for biosynthesis of mogroside compounds

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008065370A2 (en) 2006-12-01 2008-06-05 The University Of York Sesquiterpenoid modifying enzymes

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12234464B2 (en) 2018-11-09 2025-02-25 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
KR20210050044A (ko) * 2019-10-25 2021-05-07 고려대학교 산학협력단 테르펜 당쇄 부가 유도체, 이의 제조방법 및 이를 유효성분으로 함유하는 조성물
KR102362135B1 (ko) * 2019-10-25 2022-02-14 고려대학교 산학협력단 테르펜 당쇄 부가 유도체, 이의 제조방법 및 이를 유효성분으로 함유하는 조성물

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MX2014006276A (es) 2014-10-13
BR112014012543A2 (pt) 2017-06-13
AU2012342114B2 (en) 2017-12-21
CN103958693B (zh) 2016-08-24
WO2013076577A1 (en) 2013-05-30
KR20140099281A (ko) 2014-08-11
HK1200494A1 (zh) 2015-08-07
AU2012342114A1 (en) 2014-05-22
MY170686A (en) 2019-08-26
CA2853677A1 (en) 2013-05-30
SG11201401927PA (en) 2014-09-26
US9920349B2 (en) 2018-03-20
JP2014533518A (ja) 2014-12-15
US10738340B2 (en) 2020-08-11
CN103958693A (zh) 2014-07-30
US20140308698A1 (en) 2014-10-16
RU2633644C2 (ru) 2017-10-16
JP6084983B2 (ja) 2017-02-22
AU2017235907A1 (en) 2017-10-12
EP2783009A1 (en) 2014-10-01
MX342851B (es) 2016-10-14
RU2014125216A (ru) 2015-12-27
US20180237819A1 (en) 2018-08-23
EP2783009B1 (en) 2016-04-13
IN2014CN03557A (cg-RX-API-DMAC7.html) 2015-10-09

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