JP6084983B2 - モグロシド化合物の酵素的合成のための方法および材料 - Google Patents

モグロシド化合物の酵素的合成のための方法および材料 Download PDF

Info

Publication number
JP6084983B2
JP6084983B2 JP2014542951A JP2014542951A JP6084983B2 JP 6084983 B2 JP6084983 B2 JP 6084983B2 JP 2014542951 A JP2014542951 A JP 2014542951A JP 2014542951 A JP2014542951 A JP 2014542951A JP 6084983 B2 JP6084983 B2 JP 6084983B2
Authority
JP
Japan
Prior art keywords
polypeptide
ugt
mogroside
amino acid
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2014542951A
Other languages
English (en)
Japanese (ja)
Other versions
JP2014533518A5 (cg-RX-API-DMAC7.html
JP2014533518A (ja
Inventor
リウ，ヤオクァン
ヨプ イ，ジョン
ヨプ イ，ジョン
クハレ，モニカ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Evolva Holding SA
Original Assignee
Evolva AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evolva AG filed Critical Evolva AG
Publication of JP2014533518A publication Critical patent/JP2014533518A/ja
Publication of JP2014533518A5 publication Critical patent/JP2014533518A5/ja
Application granted granted Critical
Publication of JP6084983B2 publication Critical patent/JP6084983B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/20—Preparation of steroids containing heterocyclic rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids

Landscapes

  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2014542951A 2011-11-23 2012-11-19 モグロシド化合物の酵素的合成のための方法および材料 Active JP6084983B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161563303P 2011-11-23 2011-11-23
US61/563,303 2011-11-23
PCT/IB2012/002857 WO2013076577A1 (en) 2011-11-23 2012-11-19 Methods and materials for enzymatic synthesis of mogroside compounds

Publications (3)

Publication Number Publication Date
JP2014533518A JP2014533518A (ja) 2014-12-15
JP2014533518A5 JP2014533518A5 (cg-RX-API-DMAC7.html) 2016-09-29
JP6084983B2 true JP6084983B2 (ja) 2017-02-22

Family

ID=47624376

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2014542951A Active JP6084983B2 (ja) 2011-11-23 2012-11-19 モグロシド化合物の酵素的合成のための方法および材料

Country Status (14)

Country Link
US (2) US9920349B2 (cg-RX-API-DMAC7.html)
EP (1) EP2783009B1 (cg-RX-API-DMAC7.html)
JP (1) JP6084983B2 (cg-RX-API-DMAC7.html)
KR (1) KR101791597B1 (cg-RX-API-DMAC7.html)
CN (1) CN103958693B (cg-RX-API-DMAC7.html)
AU (2) AU2012342114B2 (cg-RX-API-DMAC7.html)
BR (1) BR112014012543A2 (cg-RX-API-DMAC7.html)
CA (1) CA2853677C (cg-RX-API-DMAC7.html)
IN (1) IN2014CN03557A (cg-RX-API-DMAC7.html)
MX (1) MX342851B (cg-RX-API-DMAC7.html)
MY (1) MY170686A (cg-RX-API-DMAC7.html)
RU (1) RU2633644C2 (cg-RX-API-DMAC7.html)
SG (1) SG11201401927PA (cg-RX-API-DMAC7.html)
WO (1) WO2013076577A1 (cg-RX-API-DMAC7.html)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015536157A (ja) * 2012-12-04 2015-12-21 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． モグロシド化合物の生合成のための方法および材料

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ604915A (en) 2010-06-02 2014-10-31 Evolva Nutrition Inc Recombinant production of steviol glycosides
SG10201606567TA (en) 2011-08-08 2016-10-28 Evolva Sa Recombinant production of steviol glycosides
IN2014CN03557A (cg-RX-API-DMAC7.html) 2011-11-23 2015-10-09 Evolva Sa
TW201402599A (zh) * 2012-05-30 2014-01-16 Suntory Holdings Ltd 甜菊糖苷糖苷轉化酵素及編碼該酵素之基因
CN105189771B (zh) 2013-02-11 2019-11-05 埃沃尔瓦公司 在重组宿主中有效产生甜菊醇糖苷
BR112017002783A2 (pt) 2014-08-11 2017-12-19 Evolva Sa produção de glicosídeos de esteviol em hospedeiros recombinantes
MY191735A (en) 2014-09-09 2022-07-13 Evolva Sa Production of steviol glycosides in recombinant hosts
CA2972739A1 (en) * 2014-09-11 2016-03-17 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) Methods of producing mogrosides and compositions comprising same and uses thereof
CA2963300C (en) 2014-10-01 2024-04-30 Evolva Sa Methods and materials for biosynthesis of mogroside compounds
JP6698028B2 (ja) 2014-10-17 2020-05-27 サントリーホールディングス株式会社 モグロール配糖体化酵素およびそれをコードする遺伝子
JP6850130B2 (ja) 2015-01-20 2021-03-31 サントリーホールディングス株式会社 モグロシドの調製方法
JP2018503388A (ja) 2015-01-30 2018-02-08 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． 組み換え宿主におけるステビオールグリコシドの生産
CN115851470B (zh) 2015-03-16 2025-09-02 帝斯曼知识产权资产管理有限公司 Udp-糖基转移酶
AU2016307066A1 (en) 2015-08-07 2018-02-08 Evolva Sa Production of steviol glycosides in recombinant hosts
CN108473528A (zh) 2015-10-29 2018-08-31 赛诺米克斯公司 高强度甜味剂
CN106924274B (zh) * 2015-12-29 2019-01-04 成都普睿法药物研发有限公司 葫芦烷型四环三萜类化合物抗肺纤维化应用
SG11201808856TA (en) 2016-04-13 2018-11-29 Evolva Sa Production of steviol glycosides in recombinant hosts
AU2017267214A1 (en) * 2016-05-16 2018-11-15 Evolva Sa Production of steviol glycosides in recombinant hosts
WO2017198682A1 (en) 2016-05-16 2017-11-23 Evolva Sa Production of steviol glycosides in recombinant hosts
CN109477126B (zh) * 2016-07-19 2023-06-20 三得利控股株式会社 罗汉果醇或罗汉果醇糖苷的生产方法
EP3535406A1 (en) 2016-11-07 2019-09-11 Evolva SA Production of steviol glycosides in recombinant hosts
WO2018204483A2 (en) 2017-05-03 2018-11-08 Senomyx, Inc. Methods for making high intensity sweeteners
MX2019013696A (es) 2017-05-19 2020-01-27 Coca Cola Co Composiciones edulcoradas de siamenósido i y usos de las mismas.
CA3064334A1 (en) 2017-06-02 2018-12-06 Givaudan Sa Compositions
WO2021214008A1 (en) 2020-04-20 2021-10-28 Givaudan Sa Compositions
EP3638804A1 (en) 2017-06-15 2020-04-22 Evolva SA Production of mogroside compounds in recombinant hosts
CN107189976B (zh) * 2017-07-28 2022-03-11 李华政 促进罗汉果ugt72b21基因表达的方法
KR20210089717A (ko) 2018-11-07 2021-07-16 피르메니히 인코포레이티드 고강도 감미료의 제조 방법
WO2020096905A1 (en) 2018-11-07 2020-05-14 Firmenich Incorporated Methods for making high intensity sweeteners
CN113302298B (zh) 2018-11-09 2025-02-25 银杏生物制品公司 罗汉果甙的生物合成
CN110669094B (zh) * 2019-10-14 2021-12-21 上海交通大学 罗汉果新苷及其制备方法
KR102362135B1 (ko) * 2019-10-25 2022-02-14 고려대학교 산학협력단 테르펜 당쇄 부가 유도체, 이의 제조방법 및 이를 유효성분으로 함유하는 조성물
BR112022018627A2 (pt) * 2020-03-17 2022-12-20 Coca Cola Co Sistemas e métodos inovadores de produção de mogrosídeo
GB202007984D0 (en) 2020-05-28 2020-07-15 Givaudan Sa Compositions
CN112063647B (zh) * 2020-09-17 2023-05-02 云南农业大学 酿酒酵母重组菌Cuol01的构建方法、酿酒酵母重组菌Cuol02及应用
CN116064712B (zh) * 2022-10-08 2025-05-06 中国药科大学 一种罗汉果醇的生物合成方法
CN121127603A (zh) 2023-04-20 2025-12-12 帝斯曼知识产权资产管理有限公司 用于产生罗汉果苷的菌株和方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6399321B1 (en) * 1999-08-18 2002-06-04 National Research Council Of Canada Methods for screening UDP-glucose:glycoprotein glucosyltransferase (UGGT) activity and nucleic acid encoding for UGGT
EP1510573A1 (en) * 2003-09-01 2005-03-02 Austria Wirtschaftsservice Gesellschaft mit beschränkter Haftung Method for detoxification of mycotoxins
US20080148432A1 (en) * 2005-12-21 2008-06-19 Mark Scott Abad Transgenic plants with enhanced agronomic traits
EP1871883A1 (en) 2005-03-02 2008-01-02 Metanomics GmbH Process for the production of fine chemicals
US8257948B1 (en) * 2011-02-17 2012-09-04 Purecircle Usa Method of preparing alpha-glucosyl Stevia composition
US8956677B2 (en) 2005-11-23 2015-02-17 The Coca-Cola Company High-potency sweetener composition with glucosamine and compositions sweetened therewith
RU2455854C2 (ru) 2005-11-23 2012-07-20 Дзе Кока-Кола Компани Композиция интенсивного подсластителя с глюкозамином и подслащенные ею композиции
US7927851B2 (en) 2006-03-21 2011-04-19 Vineland Research And Innovation Centre Compositions having ent-kaurenoic acid 13-hydroxylase activity and methods for producing same
US20100143975A1 (en) * 2006-11-22 2010-06-10 The University Of York Monoterpenoid modifying enzymes
WO2008065370A2 (en) * 2006-12-01 2008-06-05 The University Of York Sesquiterpenoid modifying enzymes
GB0904639D0 (en) * 2009-03-18 2009-04-29 Univ York Assay
NZ604915A (en) 2010-06-02 2014-10-31 Evolva Nutrition Inc Recombinant production of steviol glycosides
IN2014CN03557A (cg-RX-API-DMAC7.html) 2011-11-23 2015-10-09 Evolva Sa
WO2014086842A1 (en) 2012-12-04 2014-06-12 Evolva Sa Methods and materials for biosynthesis of mogroside compounds

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015536157A (ja) * 2012-12-04 2015-12-21 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． モグロシド化合物の生合成のための方法および材料

Also Published As

Publication number Publication date
CA2853677C (en) 2019-01-29
MX2014006276A (es) 2014-10-13
BR112014012543A2 (pt) 2017-06-13
AU2012342114B2 (en) 2017-12-21
CN103958693B (zh) 2016-08-24
WO2013076577A1 (en) 2013-05-30
KR20140099281A (ko) 2014-08-11
HK1200494A1 (zh) 2015-08-07
AU2012342114A1 (en) 2014-05-22
MY170686A (en) 2019-08-26
CA2853677A1 (en) 2013-05-30
SG11201401927PA (en) 2014-09-26
US9920349B2 (en) 2018-03-20
KR101791597B1 (ko) 2017-10-30
JP2014533518A (ja) 2014-12-15
US10738340B2 (en) 2020-08-11
CN103958693A (zh) 2014-07-30
US20140308698A1 (en) 2014-10-16
RU2633644C2 (ru) 2017-10-16
AU2017235907A1 (en) 2017-10-12
EP2783009A1 (en) 2014-10-01
MX342851B (es) 2016-10-14
RU2014125216A (ru) 2015-12-27
US20180237819A1 (en) 2018-08-23
EP2783009B1 (en) 2016-04-13
IN2014CN03557A (cg-RX-API-DMAC7.html) 2015-10-09

Similar Documents

Publication Publication Date Title
JP6084983B2 (ja) モグロシド化合物の酵素的合成のための方法および材料
AU2022204012B2 (en) Methods and materials for biosynthesis of mogroside compounds
AU2022205176B2 (en) Production of steviol glycosides in recombinant hosts
AU2019250137B2 (en) Methods and materials for biosynthesis of mogroside compounds
EP3332018B1 (en) Production of steviol glycosides in recombinant hosts
US20250331481A1 (en) Group of udp-glycosyltransferase for catalyzing carbohydrate chain elongation, and application thereof
JP2022047541A (ja) レバウディオサイドｄおよびレバウディオサイドｍの改良された産生方法
Erthmann et al. A tandem array of UDP-glycosyltransferases from the UGT73C subfamily glycosylate sapogenins, forming a spectrum of mono-and bisdesmosidic saponins
Lyu et al. Identification and characterization of three flavonoid 3-O-glycosyltransferases from Epimedium koreanum Nakai
CN104357418A (zh) 一种糖基转移酶及其突变体在合成人参皂苷Rh2中的应用
CN105087739A (zh) 一种新的制备稀有人参皂苷的催化体系及其应用
WO2021147575A1 (zh) 新型碳苷糖基转移酶及其应用
WO2018083338A1 (en) Production of steviol glycosides in recombinant hosts
Yang et al. Systems metabolic engineering of Escherichia coli to enhance the production of flavonoid glucuronides
US12454714B2 (en) Monbretin A (MbA) synthesis using a heterologous nucleic acid(s) encoding A MbA pathway enzyme
HK1200494B (en) Methods and materials for enzymatic synthesis of mogroside compounds
Xu et al. Convergent Evolution and De Novo Reconstitution of Apiin Biosynthesis
KR20110029372A (ko) 산소-메틸화전이효소 변이체

Legal Events

Date Code Title Description
A529 Written submission of copy of amendment under article 34 pct

Free format text: JAPANESE INTERMEDIATE CODE: A529

Effective date: 20140718

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20151119

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20151119

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20151228

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160809

A871 Explanation of circumstances concerning accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A871

Effective date: 20160809

A975 Report on accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A971005

Effective date: 20160817

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20160906

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20161206

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20170117

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20170126

R150 Certificate of patent or registration of utility model

Ref document number: 6084983

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

S111 Request for change of ownership or part of ownership

Free format text: JAPANESE INTERMEDIATE CODE: R313111

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350