KR0180097B1 - Method of cultivating phellinus linteus - Google Patents

Abstract

The situation in the wild in mulberry trees According to the research result that mushrooms are effective in inhibiting the growth of cancer cells, the demand of the mushrooms has increased so that the inventor has observed the growth of mushrooms and observed the growth of the mushrooms and made efforts to artificial cultivation using mulberry trees, After the sterilization process, inoculation process, bacterial specification process, and low temperature impact process, mushroom foot process was established to establish artificial cultivation method of mushroom.

Description

Artificial cultivation method of field mushroom wood

The present invention relates to a method for artificial cultivation of logs of mushrooms.

Mushroom is a mushroom generally known to be harvested from wild mulberry trees. It has recently been shown through various clinical trials that it has the effect of inhibiting the growth of cancer cells, but due to its rarity, it is difficult to meet demand.

The mushroom refers to Phellinus linteus which is parasitic to the mulberry, also known as mulberry ear, stratum corneum, and Hosonan, and belongs to the pine scaly mushroom (Hymenochaltaclal) It has long been used as a treatment for women's diseases such as hemorrhage of the uterus and menstrual irregularities. Recently, it has been known that the tumor inhibition rate is the most superior to other mushrooms (eg mulberry mushroom and mulberry mushroom) .

In general, mushrooms are known to be parasitic on mulberry trees. However, it has been found that the mushrooms are wild in broad-leaved trees such as ducks, according to the researches conducted in Korea. In recent years, Is known.

However, the mushroom is a rare plant that can be collected occasionally in the rugged mountainous region due to limited occurrence conditions and environment. It is a wild plant and grows toward the ground. Therefore, it grows in the stems or branches above the root of the plant. And it is difficult to collect a large amount of the drug despite the effectiveness of the drug because the drug must be continuously grown for 3 to 4 years. The present inventors have succeeded in establishing the most suitable growth conditions of the field mushroom after many years of research and experiment.

Accordingly, it is an object of the present invention to provide a cultivation method capable of artificially mass-cultivating environment-friendly mushrooms having excellent hygienic characteristics.

In order to accomplish the above object, the present invention provides a method of growing a mushroom of the present invention comprising cutting a mulberry tree, oak tree or oak tree having a moisture content of 40 to 50% into a length of 20 cm, sealing the same in a plastic bag, A sterilization step of heating for a period of time to 20 hours; After the sterilization process, open the upper side of the seal, put 50 ~ 80g of the mushroom seeds on the cutting surface, fix the PVC tube of 2.5cm ~ 3cm length on the sealing part to cover the upper side of the suture with the cotton wool, Seal seeding inoculation process; A mycelial culturing step in which the seedling is inoculated in a device capable of controlling temperature after completion of the seedling inoculation and is maintained at 23 ° C to 25 ° C for the first month and at 25 ° C to 28 ° C for the remaining 2 to 3 months;

After the mycelial cultivation process, the envelope of the encapsulated cultivar was sealed and the seeds adhered to the area below 4 ~ 5cm from the inoculated upper log cut surface were cleanly removed and then adjusted to a humidity of 90% or more and a temperature of 25 ~ It is cultivated with a mushroom foot process planted so that it is planted so that it is raised up to the ground and the moisture is at least 90% and the temperature is kept at 25 ° C to 30 ° C. .

Hereinafter, the present invention will be described in detail.

The present inventor has conceived a method of culturing using seed bacterium. In order to select a seed for culturing the seed bacterium first, the hardwood is collected in addition to the mulberry tree, and the seed bacterium is inoculated after the pretreatment by various methods. The oak tree species such as oak tree, , Oak tree, oak tree, chestnut tree, or orelic tree. In other words, mulberry was the best, but the most easy to obtain were oak and orel. (Approximately from November to February). It is natural to dry in the shade after logging, and when the moisture of the wood is 30 ~ 40%, the length of the wood is cut to a length of 20 ~ 30cm, Vinyl, etc., and it is heated at a temperature of about 90 ° C or more for a long time to be sterilized. The sterilization time is about 10 hours But it is most preferable to be 15 to 20 hours.

After the sterilization process is completed, the upper part of the sealing section is opened, and an appropriate amount of the mushroom seeds is placed on the cutting section of the log, and then it is sealed again. The PVC tube is cut so that it can be blocked by the cotton wool, etc., and fixed to the sealing vinyl, End the inoculation process. At this time, the amount of mushroom seeds varies depending on the diameter of the wood, but the diameter of the wood is about 10 cm to 20 cm, preferably about 50 g to 100 g.

After inoculating seeds into logs and keeping them in a temperature-controlled facility, they should be stored at 23 ℃ ~ 25 ℃ for the first month and then kept at 25 ℃ ~ 28 ℃ for the first month. Later, we can observe that mycelium spreads in the wood. After finishing the mycelial culturing process, remove the cotton plug and remove the wrapped plastic such as vinyl, and the seed which was first used as the inoculation source on the upper part is cleaned with a chisel and then the mushroom foot goes into the process so that the humidity is about 90% It is planted in soil which can be controlled at ~ 30 ℃. It is planted by leaving about 5-7cm from the cutting surface of the log, and the lower part is planted in the soil. After planting, watering with a small amount of once to three times a day, From days to about 20 days, fruiting bodies of the mushroom were formed in the logs and succeeded in artificial cultivation of the mushrooms.

The reason why the mushroom feet should be planted in a soil which can be controlled at a temperature of about 25 ° C to 30 ° C in the process is that the mushroom does not grow well and does not grow well when the temperature of the soil is below 25 ° C, If the temperature is lower than 30 ° C, the mushroom will not grow well and will not grow well. Also, if the temperature of the soil is higher than 30 ° C, the mushroom will be damaged.

On the other hand, after completion of the mycelial cultivation process, it was put in a device capable of controlling the temperature below -5 ° C, and subjected to a low-temperature impact process for about 15 days. Then, the pavement was removed and planted in the soil as described above. Cultivation of mushrooms was more effective and desirable because mushroom development was even more uniform.

The low temperature impact process showed some difference depending on the treatment period, but it was most preferable to treat for about 15 days.

The mushroom feet are more suitable for the soil that is planted by planting the mycelial cultivation log in the process, so that the soil is mixed with some sand or margarine to drain well.

The artificial cultivation method of the mushroom of the present invention will be described in detail as an example.

[Example 1]

The cut oak (diameter 13 cm) cut into 20 cm length at the end of November and left in the shade for about 2 to 3 days, cut the vinyl foil (thickness 0.02 mm) into 1 mm length, (Weighing 3.1 kg) was put uprightly, and the upper part was bundled and sealed, and the wood cutting and the vinyl coating process were completed, followed by sterilization by heating at about 100 ° C for 20 hours. Uncouple the upper bundle, put 50g of the seeds in the upper part of the logs, spread it, spread the plastic tube of 2.5cm inside diameter 3cm in length and put the washer in the plastic bag.

This was kept in a temperature-controlled facility, and the temperature was controlled. The mixture was maintained at 23 ° C to 25 ° C for 30 days and then at 26 ° C to 28 ° C for 60 days. After the mycelial cultivation process was finished, the seeds of the cuttings of the logs were removed from the wrapped plastic bags and the seedlings of the thorns less than 5 cm below the cutting surface were carefully scratched off to prevent the epidermis from being scratched and then planted in the soil of the three- . The soil was drained well and the shade net was doubled in order to control the rapid temperature and humidity changes, and the house vinyl was doubled. In the middle, cotton (caissimiron) was added to protect the day and night temperature drift.

The logs that were planted in soil in the vinyl house were cultivated 5cm ~ 7cm above the soil and sand was placed on the cut surface to promote the growth of humidity and the growth of mushroom on the logs. The mushroom's foot grows through the process by adjusting the humidity to 90 ° C and the temperature to 26 ° C to 30 ° C. The mushroom was light-yellow semicircular to flat or horseshoe-shaped, and there was no stand. When this was grown for two years, the light yellow color gradually faded and turned yellow, and the weight of the mushroom per harvest was as follows.

Artificial cultivation yield of field mushroom (per 1 log)

One year after 32g (light yellow)

After 2 years 61g (light yellow to light brown)

After 3 years 93g (light yellow to dark brown)

[Example 2]

After the sterilization process for cutting the logs and the vinyl coating process, the inoculation process and the mycelium cultivation process were completed in the same manner as in Example 1, the cells were subjected to a low-temperature impact process for 2 weeks in a facility stored at -5 캜 or lower, The condition of the mushroom foot was artificially cultivated by the process. The growth of the mushroom was better, and the weight of the mushroom per harvest was as follows.

Artificial cultivation yield of field mushroom (per 1 log)

After 1 year 35g (light yellow)

After 2 years 70g (light yellow to light brown)

After 3 years 106g (light yellow to dark brown)

Generally, it is preferable to cultivate perennial mushroom if possible because the efficacy of the mushroom is more prominent in perennials.

Recently, the inventor of the present invention invented the artificial cultivation method of the mushroom by cultivating the seed bacterium in the oak or the duck tree, so that the mushroom is very rare in anticipation as a wild animal parasitic on the mulberry tree. The effect that contributes to is great.

Claims (2)

A sterilization step in which the wood of mulberry, oak, or orelice having a moisture content of 40 to 50% is cut into a length of 20 cm and heated in 90 ° C or higher for 15 to 20 hours in a plastic bag or the like; A seedling seeding step of putting 50 to 80 g of the mushroom seedlings on the cutting surface with the upper side open, placing a PVC tube of 2.5 cm to 3 cm length on the upper side of the sealing part to seal the upper side of the sealing part with a cotton pad, A mycelial culturing step in which the seedling is inoculated in a device capable of controlling temperature after completion of the seedling inoculation and is maintained at 23 ° C to 25 ° C for the first month and at 25 ° C to 28 ° C for the remaining 2 to 3 months; After the mycelial cultivation process, the envelope of the encapsulated cultivar was sealed and the seeds adhered to the area below 4 ~ 5cm from the inoculated upper log cut surface were cleanly removed and then adjusted to a humidity of 90% or more and a temperature of 25 ~ It is cultivated by planting in the soil as possible, planting it 5 to 7 cm below the top of the cut, and keeping the moisture at 90% or higher and temperature at 25 to 30 ° C. A method of artificial cultivation of a field mushroom. The method according to claim 1, wherein the low temperature impact process is carried out at a temperature of -5 ° C or lower for 15 days after the cultivation of the mycelium.