JPWO2020204055A1 - - Google Patents
Info
- Publication number
- JPWO2020204055A1 JPWO2020204055A1 JP2021512163A JP2021512163A JPWO2020204055A1 JP WO2020204055 A1 JPWO2020204055 A1 JP WO2020204055A1 JP 2021512163 A JP2021512163 A JP 2021512163A JP 2021512163 A JP2021512163 A JP 2021512163A JP WO2020204055 A1 JPWO2020204055 A1 JP WO2020204055A1
- Authority
- JP
- Japan
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2866—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/52—Constant or Fc region; Isotype
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2019070329 | 2019-04-02 | ||
| JP2019070329 | 2019-04-02 | ||
| PCT/JP2020/014980 WO2020204055A1 (ja) | 2019-04-02 | 2020-04-01 | 標的特異的な外来遺伝子の導入方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JPWO2020204055A1 true JPWO2020204055A1 (https=) | 2020-10-08 |
| JPWO2020204055A5 JPWO2020204055A5 (https=) | 2024-01-23 |
| JP7522100B2 JP7522100B2 (ja) | 2024-07-24 |
Family
ID=72668649
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021512163A Active JP7522100B2 (ja) | 2019-04-02 | 2020-04-01 | 標的特異的な外来遺伝子の導入方法 |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US12344859B2 (https=) |
| EP (1) | EP3950938A4 (https=) |
| JP (1) | JP7522100B2 (https=) |
| KR (1) | KR102655024B1 (https=) |
| CN (1) | CN113661238B (https=) |
| AU (1) | AU2020252840B2 (https=) |
| CA (1) | CA3134705A1 (https=) |
| IL (1) | IL286630B2 (https=) |
| MX (1) | MX2021011903A (https=) |
| SG (1) | SG11202110076PA (https=) |
| TW (1) | TWI851703B (https=) |
| WO (1) | WO2020204055A1 (https=) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2025216715A1 (en) * | 2024-04-12 | 2025-10-16 | Agency For Science, Technology And Research | Identification of transcriptional hotspots in cho cells |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002526071A (ja) * | 1998-09-22 | 2002-08-20 | ブリストル−マイヤーズ スクイブ カンパニー | トランスフェクションした細胞中の組換えタンパク質発現増大のためのホットスポットを含む発現ベクター |
| WO2008151219A1 (en) * | 2007-06-04 | 2008-12-11 | Regeneron Pharmaceuticals, Inc. | Enhanced expression and stability regions |
| JP2015519914A (ja) * | 2012-06-22 | 2015-07-16 | ロンザ バイオロジクス ピーエルシー | 部位特異的組込み |
| JP2017535258A (ja) * | 2014-10-23 | 2017-11-30 | リジェネロン・ファーマシューティカルズ・インコーポレイテッドＲｅｇｅｎｅｒｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． | 新規ｃｈｏ組込み部位およびその使用 |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2707189B1 (fr) | 1993-07-09 | 1995-10-13 | Gradient Ass | Procédé de traitement de résidus de combustion et installation de mise en Óoeuvre dudit procédé. |
| US6800457B2 (en) | 1998-09-22 | 2004-10-05 | Bristol-Myers Squibb Company | Expression vectors containing hot spot for increased recombinant protein expression in transfected cells |
| CN1182244C (zh) * | 1999-05-10 | 2004-12-29 | 中外制药株式会社 | 培养细胞的方法 |
| DE60140474D1 (de) | 2000-09-08 | 2009-12-24 | Univ Zuerich | Sammlung von proteinen mit sich wiederholenden sequenzen (repeat proteins), die repetitive sequenzmodule enthalten |
| CA2418835A1 (en) | 2000-10-16 | 2002-04-25 | Phylos, Inc. | Protein scaffolds for antibody mimics and other binding proteins |
| US20030157561A1 (en) | 2001-11-19 | 2003-08-21 | Kolkman Joost A. | Combinatorial libraries of monomer domains |
| WO2003029462A1 (en) | 2001-09-27 | 2003-04-10 | Pieris Proteolab Ag | Muteins of human neutrophil gelatinase-associated lipocalin and related proteins |
| WO2005040229A2 (en) | 2003-10-24 | 2005-05-06 | Avidia, Inc. | Ldl receptor class a and egf domain monomers and multimers |
| CN1618955A (zh) * | 2003-11-20 | 2005-05-25 | 中国人民解放军军事医学科学院生物工程研究所 | 一种CHO/dhfr－细胞定点整合表达系统 |
| JP4818936B2 (ja) * | 2005-01-05 | 2011-11-16 | 中外製薬株式会社 | 細胞の培養方法およびその利用 |
| JP2007325571A (ja) | 2006-06-09 | 2007-12-20 | Toto Ltd | 組換え哺乳類動物細胞を用いたタンパク質生産方法 |
| CA2659574C (en) | 2006-08-02 | 2017-04-25 | The Uab Research Foundation | Methods and compositions related to soluble monoclonal variable lymphocyte receptors of defined antigen specificity |
| EP3176263A1 (en) | 2007-08-10 | 2017-06-07 | Toto Ltd. | Method of producing recombinant mammalian cells |
| US8252557B2 (en) * | 2008-10-28 | 2012-08-28 | Chugai Seiyaku Kabushiki Kaisha | Peptide-containing culture medium for culturing animal cell |
| JP2010210772A (ja) | 2009-03-13 | 2010-09-24 | Dainippon Screen Mfg Co Ltd | 液晶表示装置の製造方法 |
| US20130034875A1 (en) * | 2009-10-01 | 2013-02-07 | Toto Ltd. | Dna construct, and process for production of recombinant cho cell using same |
| AU2012240164A1 (en) | 2011-04-05 | 2013-11-14 | The Scripps Research Institute | Chromosomal landing pads and related uses |
| BR112015031639A2 (pt) | 2013-06-19 | 2019-09-03 | Sigma Aldrich Co Llc | integração alvo |
| WO2015092735A1 (en) * | 2013-12-20 | 2015-06-25 | Novartis Ag | Novel eukaryotic cells and methods for recombinantly expressing a product of interest |
| JP6858559B2 (ja) | 2014-08-20 | 2021-04-14 | 中外製薬株式会社 | 蛋白質溶液の粘度測定方法 |
| CN104818253B (zh) * | 2015-03-24 | 2018-03-09 | 成都贝爱特生物科技有限公司 | 靶向定点整合cho细胞系的改造及其用途 |
| DE102015222931A1 (de) * | 2015-11-20 | 2017-05-24 | Continental Teves Ag & Co. Ohg | Anordnung zur Messung eines durch Bremskraft erzeugten Verschiebeweges |
| EA201892137A1 (ru) | 2016-04-20 | 2019-03-29 | Регенерон Фармасьютикалз, Инк. | Композиции и способы получения антител, основанные на применении локусов, обеспечивающих повышенную экспрессию |
| US11512144B2 (en) | 2016-04-20 | 2022-11-29 | Regeneran Pharmaceuticals, Inc. | Compositions and methods for making antibodies based on use of an expression-enhancing loci |
| EP4130280A3 (en) | 2016-12-20 | 2023-04-19 | Development Center for Biotechnology | Targeted integration sites in chinese hamster ovary cell genome |
| SG11202000966XA (en) * | 2017-08-11 | 2020-02-27 | Boehringer Ingelheim Int | Integration sites in CHO cells |
-
2020
- 2020-04-01 KR KR1020217034905A patent/KR102655024B1/ko active Active
- 2020-04-01 JP JP2021512163A patent/JP7522100B2/ja active Active
- 2020-04-01 WO PCT/JP2020/014980 patent/WO2020204055A1/ja not_active Ceased
- 2020-04-01 AU AU2020252840A patent/AU2020252840B2/en active Active
- 2020-04-01 SG SG11202110076PA patent/SG11202110076PA/en unknown
- 2020-04-01 MX MX2021011903A patent/MX2021011903A/es unknown
- 2020-04-01 CN CN202080026711.XA patent/CN113661238B/zh active Active
- 2020-04-01 US US17/600,319 patent/US12344859B2/en active Active
- 2020-04-01 TW TW109111209A patent/TWI851703B/zh active
- 2020-04-01 CA CA3134705A patent/CA3134705A1/en active Pending
- 2020-04-01 EP EP20784329.3A patent/EP3950938A4/en active Pending
- 2020-04-01 IL IL286630A patent/IL286630B2/en unknown
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002526071A (ja) * | 1998-09-22 | 2002-08-20 | ブリストル−マイヤーズ スクイブ カンパニー | トランスフェクションした細胞中の組換えタンパク質発現増大のためのホットスポットを含む発現ベクター |
| WO2008151219A1 (en) * | 2007-06-04 | 2008-12-11 | Regeneron Pharmaceuticals, Inc. | Enhanced expression and stability regions |
| JP2015519914A (ja) * | 2012-06-22 | 2015-07-16 | ロンザ バイオロジクス ピーエルシー | 部位特異的組込み |
| JP2017535258A (ja) * | 2014-10-23 | 2017-11-30 | リジェネロン・ファーマシューティカルズ・インコーポレイテッドＲｅｇｅｎｅｒｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． | 新規ｃｈｏ組込み部位およびその使用 |
Non-Patent Citations (1)
| Title |
|---|
| "Database GenBank [online], NCBI Reference Sequence: NW_003614838.1", DEFINITION: CRICETULUS GRISEUS UNPLACED GENOMIC SCAFFOLD, CRIGRI_1.0 SCAFFOLD6779, WHOLE GENOME SHOT, JPN7020001640, ISSN: 0005337016 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113661238A (zh) | 2021-11-16 |
| EP3950938A4 (en) | 2023-01-18 |
| IL286630B2 (en) | 2025-05-01 |
| US20220170047A1 (en) | 2022-06-02 |
| BR112021018313A2 (pt) | 2021-11-23 |
| MX2021011903A (es) | 2021-10-26 |
| CA3134705A1 (en) | 2020-10-08 |
| AU2020252840A1 (en) | 2021-11-04 |
| KR20210146362A (ko) | 2021-12-03 |
| CN113661238B (zh) | 2024-08-27 |
| IL286630A (en) | 2021-12-01 |
| TWI851703B (zh) | 2024-08-11 |
| US12344859B2 (en) | 2025-07-01 |
| SG11202110076PA (en) | 2021-10-28 |
| IL286630B1 (en) | 2025-01-01 |
| KR102655024B1 (ko) | 2024-04-04 |
| EP3950938A1 (en) | 2022-02-09 |
| AU2020252840B2 (en) | 2025-01-23 |
| WO2020204055A1 (ja) | 2020-10-08 |
| JP7522100B2 (ja) | 2024-07-24 |
| TW202043478A (zh) | 2020-12-01 |
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