CN113661238B - 引入靶特异性外源基因的方法 - Google Patents

引入靶特异性外源基因的方法 Download PDF

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Publication number
CN113661238B
CN113661238B CN202080026711.XA CN202080026711A CN113661238B CN 113661238 B CN113661238 B CN 113661238B CN 202080026711 A CN202080026711 A CN 202080026711A CN 113661238 B CN113661238 B CN 113661238B
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dna
cells
exogenous dna
gene
region
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CN113661238A (zh
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小松将大
小村健太
若原裕二
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Chugai Pharmaceutical Co Ltd
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Chugai Pharmaceutical Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2866—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
CN202080026711.XA 2019-04-02 2020-04-01 引入靶特异性外源基因的方法 Active CN113661238B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019-070329 2019-04-02
JP2019070329 2019-04-02
PCT/JP2020/014980 WO2020204055A1 (ja) 2019-04-02 2020-04-01 標的特異的な外来遺伝子の導入方法

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CN113661238A CN113661238A (zh) 2021-11-16
CN113661238B true CN113661238B (zh) 2024-08-27

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US (1) US12344859B2 (https=)
EP (1) EP3950938A4 (https=)
JP (1) JP7522100B2 (https=)
KR (1) KR102655024B1 (https=)
CN (1) CN113661238B (https=)
AU (1) AU2020252840B2 (https=)
CA (1) CA3134705A1 (https=)
IL (1) IL286630B2 (https=)
MX (1) MX2021011903A (https=)
SG (1) SG11202110076PA (https=)
TW (1) TWI851703B (https=)
WO (1) WO2020204055A1 (https=)

Families Citing this family (1)

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Publication number Priority date Publication date Assignee Title
WO2025216715A1 (en) * 2024-04-12 2025-10-16 Agency For Science, Technology And Research Identification of transcriptional hotspots in cho cells

Citations (1)

* Cited by examiner, † Cited by third party
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CN104818253A (zh) * 2015-03-24 2015-08-05 成都贝爱特生物科技有限公司 靶向定点整合cho细胞系的改造及其用途

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DE60021373T2 (de) * 1999-05-10 2006-04-27 Chugai Seiyaku K.K. Verfahren der zellkultivierung
EP1332209B1 (en) 2000-09-08 2009-11-11 Universität Zürich Collections of repeat proteins comprising repeat modules
EP2141243A3 (en) 2000-10-16 2010-01-27 Brystol-Myers Squibb Company Protein scaffolds for antibody mimics and other binding proteins
US20030157561A1 (en) 2001-11-19 2003-08-21 Kolkman Joost A. Combinatorial libraries of monomer domains
WO2003029462A1 (en) 2001-09-27 2003-04-10 Pieris Proteolab Ag Muteins of human neutrophil gelatinase-associated lipocalin and related proteins
US20050164301A1 (en) 2003-10-24 2005-07-28 Avidia Research Institute LDL receptor class A and EGF domain monomers and multimers
CN1618955A (zh) 2003-11-20 2005-05-25 中国人民解放军军事医学科学院生物工程研究所 一种CHO/dhfr－细胞定点整合表达系统
EP1835022B1 (en) * 2005-01-05 2015-02-11 Chugai Seiyaku Kabushiki Kaisha Cell culture method and utilization of the same
JP2007325571A (ja) 2006-06-09 2007-12-20 Toto Ltd 組換え哺乳類動物細胞を用いたタンパク質生産方法
CA2659574C (en) 2006-08-02 2017-04-25 The Uab Research Foundation Methods and compositions related to soluble monoclonal variable lymphocyte receptors of defined antigen specificity
EP2150617B1 (en) * 2007-06-04 2014-10-22 Regeneron Pharmaceuticals, Inc. Enhanced expression and stability regions
US8071332B2 (en) 2007-08-10 2011-12-06 Toto Ltd. Recombinant mammal cells, method of producing thereof, and method of producing proteins of interest
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Publication number Publication date
CA3134705A1 (en) 2020-10-08
IL286630A (en) 2021-12-01
JPWO2020204055A1 (https=) 2020-10-08
CN113661238A (zh) 2021-11-16
AU2020252840B2 (en) 2025-01-23
BR112021018313A2 (pt) 2021-11-23
SG11202110076PA (en) 2021-10-28
KR102655024B1 (ko) 2024-04-04
TW202043478A (zh) 2020-12-01
US20220170047A1 (en) 2022-06-02
US12344859B2 (en) 2025-07-01
KR20210146362A (ko) 2021-12-03
AU2020252840A1 (en) 2021-11-04
IL286630B1 (en) 2025-01-01
WO2020204055A1 (ja) 2020-10-08
TWI851703B (zh) 2024-08-11
EP3950938A1 (en) 2022-02-09
EP3950938A4 (en) 2023-01-18
JP7522100B2 (ja) 2024-07-24
MX2021011903A (es) 2021-10-26
IL286630B2 (en) 2025-05-01

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