SG11202110076PA - Method of introducing target-specific foreign gene - Google Patents

Method of introducing target-specific foreign gene

Info

Publication number
SG11202110076PA
SG11202110076PA SG11202110076PA SG11202110076PA SG11202110076PA SG 11202110076P A SG11202110076P A SG 11202110076PA SG 11202110076P A SG11202110076P A SG 11202110076PA SG 11202110076P A SG11202110076P A SG 11202110076PA SG 11202110076P A SG11202110076P A SG 11202110076PA
Authority
SG
Singapore
Prior art keywords
foreign gene
specific foreign
introducing target
introducing
target
Prior art date
Application number
SG11202110076PA
Inventor
Shodai Komatsu
Kenta Komura
Yuuji Wakahara
Original Assignee
Chugai Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chugai Pharmaceutical Co Ltd filed Critical Chugai Pharmaceutical Co Ltd
Publication of SG11202110076PA publication Critical patent/SG11202110076PA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2866Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/10Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14Specific host cells or culture conditions, e.g. components, pH or temperature
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52Constant or Fc region; Isotype

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
SG11202110076PA 2019-04-02 2020-04-01 Method of introducing target-specific foreign gene SG11202110076PA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2019070329 2019-04-02
PCT/JP2020/014980 WO2020204055A1 (en) 2019-04-02 2020-04-01 Method of introducing target-specific foreign gene

Publications (1)

Publication Number Publication Date
SG11202110076PA true SG11202110076PA (en) 2021-10-28

Family

ID=72668649

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202110076PA SG11202110076PA (en) 2019-04-02 2020-04-01 Method of introducing target-specific foreign gene

Country Status (13)

Country Link
US (1) US20220170047A1 (en)
EP (1) EP3950938A4 (en)
JP (1) JPWO2020204055A1 (en)
KR (1) KR102655024B1 (en)
CN (1) CN113661238A (en)
AU (1) AU2020252840A1 (en)
BR (1) BR112021018313A2 (en)
CA (1) CA3134705A1 (en)
IL (1) IL286630A (en)
MX (1) MX2021011903A (en)
SG (1) SG11202110076PA (en)
TW (1) TW202043478A (en)
WO (1) WO2020204055A1 (en)

Family Cites Families (23)

* Cited by examiner, † Cited by third party
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US6521419B1 (en) 1998-09-22 2003-02-18 Kanakaraju Koduri Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
WO2002020565A2 (en) 2000-09-08 2002-03-14 Universität Zürich Collections of repeat proteins comprising repeat modules
CA2418835A1 (en) 2000-10-16 2002-04-25 Phylos, Inc. Protein scaffolds for antibody mimics and other binding proteins
US20030157561A1 (en) 2001-11-19 2003-08-21 Kolkman Joost A. Combinatorial libraries of monomer domains
WO2003029462A1 (en) 2001-09-27 2003-04-10 Pieris Proteolab Ag Muteins of human neutrophil gelatinase-associated lipocalin and related proteins
US20050164301A1 (en) 2003-10-24 2005-07-28 Avidia Research Institute LDL receptor class A and EGF domain monomers and multimers
CN1618955A (en) * 2003-11-20 2005-05-25 中国人民解放军军事医学科学院生物工程研究所 CHO/dhfr cell fixed point integrated expression system
DK2049564T3 (en) 2006-08-02 2017-07-24 Uab Res Found METHODS AND COMPOSITIONS RELATED TO SOLUBLE MONOCLONAL VARIABLE Lymphocyte RECEPTORS WITH DEFINED ANTIGEN SPECIFICITY
WO2008151219A1 (en) 2007-06-04 2008-12-11 Regeneron Pharmaceuticals, Inc. Enhanced expression and stability regions
JP2010210772A (en) 2009-03-13 2010-09-24 Dainippon Screen Mfg Co Ltd Method of manufacturing liquid crystal display device
US20130034875A1 (en) * 2009-10-01 2013-02-07 Toto Ltd. Dna construct, and process for production of recombinant cho cell using same
KR101999410B1 (en) * 2011-04-05 2019-07-11 더 스크립스 리서치 인스티튜트 Chromosomal landing pads and related uses
EP2711428A1 (en) * 2012-09-21 2014-03-26 Lonza Biologics plc. Site-specific integration
KR102288857B1 (en) 2013-12-20 2021-08-11 노파르티스 아게 Novel eukaryotic cells and methods for recombinantly expressing a product of interest
JP6858559B2 (en) 2014-08-20 2021-04-14 中外製薬株式会社 Method for measuring viscosity of protein solution
EA037255B1 (en) 2014-10-23 2021-02-26 Регенерон Фармасьютикалз, Инк. Novel cho integration sites and uses thereof
CN104818253B (en) * 2015-03-24 2018-03-09 成都贝爱特生物科技有限公司 Target transformation of site-directed integration Chinese hamster ovary celI system and application thereof
DE102015222931A1 (en) * 2015-11-20 2017-05-24 Continental Teves Ag & Co. Ohg Arrangement for measuring a displacement path generated by braking force
TWI827531B (en) * 2016-04-20 2024-01-01 美商再生元醫藥公司 Compositions and methods for making antibodies based on use of expression-enhancing loci
JP7134868B2 (en) 2016-04-20 2022-09-12 リジェネロン・ファーマシューティカルズ・インコーポレイテッド Compositions and methods for generating antibodies based on the use of enhanced expression loci
WO2018118901A1 (en) * 2016-12-20 2018-06-28 Development Center For Biotechnology Targeted integration sites in chinese hamster ovary cell genome
EP3665291B1 (en) * 2017-08-11 2021-10-06 Boehringer Ingelheim International GmbH Integration sites in cho cells

Also Published As

Publication number Publication date
KR102655024B1 (en) 2024-04-04
EP3950938A1 (en) 2022-02-09
US20220170047A1 (en) 2022-06-02
IL286630A (en) 2021-12-01
WO2020204055A1 (en) 2020-10-08
TW202043478A (en) 2020-12-01
CN113661238A (en) 2021-11-16
CA3134705A1 (en) 2020-10-08
AU2020252840A1 (en) 2021-11-04
EP3950938A4 (en) 2023-01-18
BR112021018313A2 (en) 2021-11-23
MX2021011903A (en) 2021-10-26
KR20210146362A (en) 2021-12-03
JPWO2020204055A1 (en) 2020-10-08

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