TWI851703B - 標的特異性外源基因的導入方法 - Google Patents
標的特異性外源基因的導入方法 Download PDFInfo
- Publication number
- TWI851703B TWI851703B TW109111209A TW109111209A TWI851703B TW I851703 B TWI851703 B TW I851703B TW 109111209 A TW109111209 A TW 109111209A TW 109111209 A TW109111209 A TW 109111209A TW I851703 B TWI851703 B TW I851703B
- Authority
- TW
- Taiwan
- Prior art keywords
- exogenous dna
- dna
- region
- cells
- introducing
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2866—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/52—Constant or Fc region; Isotype
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2019-070329 | 2019-04-02 | ||
| JP2019070329 | 2019-04-02 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| TW202043478A TW202043478A (zh) | 2020-12-01 |
| TWI851703B true TWI851703B (zh) | 2024-08-11 |
Family
ID=72668649
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TW109111209A TWI851703B (zh) | 2019-04-02 | 2020-04-01 | 標的特異性外源基因的導入方法 |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US12344859B2 (https=) |
| EP (1) | EP3950938A4 (https=) |
| JP (1) | JP7522100B2 (https=) |
| KR (1) | KR102655024B1 (https=) |
| CN (1) | CN113661238B (https=) |
| AU (1) | AU2020252840B2 (https=) |
| CA (1) | CA3134705A1 (https=) |
| IL (1) | IL286630B2 (https=) |
| MX (1) | MX2021011903A (https=) |
| SG (1) | SG11202110076PA (https=) |
| TW (1) | TWI851703B (https=) |
| WO (1) | WO2020204055A1 (https=) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2025216715A1 (en) * | 2024-04-12 | 2025-10-16 | Agency For Science, Technology And Research | Identification of transcriptional hotspots in cho cells |
Family Cites Families (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2707189B1 (fr) | 1993-07-09 | 1995-10-13 | Gradient Ass | Procédé de traitement de résidus de combustion et installation de mise en Óoeuvre dudit procédé. |
| US6800457B2 (en) | 1998-09-22 | 2004-10-05 | Bristol-Myers Squibb Company | Expression vectors containing hot spot for increased recombinant protein expression in transfected cells |
| US6521419B1 (en) | 1998-09-22 | 2003-02-18 | Kanakaraju Koduri | Expression vectors containing hot spot for increased recombinant protein expression in transfected cells |
| DE60021373T2 (de) * | 1999-05-10 | 2006-04-27 | Chugai Seiyaku K.K. | Verfahren der zellkultivierung |
| EP1332209B1 (en) | 2000-09-08 | 2009-11-11 | Universität Zürich | Collections of repeat proteins comprising repeat modules |
| EP2141243A3 (en) | 2000-10-16 | 2010-01-27 | Brystol-Myers Squibb Company | Protein scaffolds for antibody mimics and other binding proteins |
| US20030157561A1 (en) | 2001-11-19 | 2003-08-21 | Kolkman Joost A. | Combinatorial libraries of monomer domains |
| WO2003029462A1 (en) | 2001-09-27 | 2003-04-10 | Pieris Proteolab Ag | Muteins of human neutrophil gelatinase-associated lipocalin and related proteins |
| US20050164301A1 (en) | 2003-10-24 | 2005-07-28 | Avidia Research Institute | LDL receptor class A and EGF domain monomers and multimers |
| CN1618955A (zh) | 2003-11-20 | 2005-05-25 | 中国人民解放军军事医学科学院生物工程研究所 | 一种CHO/dhfr－细胞定点整合表达系统 |
| EP1835022B1 (en) * | 2005-01-05 | 2015-02-11 | Chugai Seiyaku Kabushiki Kaisha | Cell culture method and utilization of the same |
| JP2007325571A (ja) | 2006-06-09 | 2007-12-20 | Toto Ltd | 組換え哺乳類動物細胞を用いたタンパク質生産方法 |
| CA2659574C (en) | 2006-08-02 | 2017-04-25 | The Uab Research Foundation | Methods and compositions related to soluble monoclonal variable lymphocyte receptors of defined antigen specificity |
| EP2150617B1 (en) * | 2007-06-04 | 2014-10-22 | Regeneron Pharmaceuticals, Inc. | Enhanced expression and stability regions |
| US8071332B2 (en) | 2007-08-10 | 2011-12-06 | Toto Ltd. | Recombinant mammal cells, method of producing thereof, and method of producing proteins of interest |
| US8252557B2 (en) * | 2008-10-28 | 2012-08-28 | Chugai Seiyaku Kabushiki Kaisha | Peptide-containing culture medium for culturing animal cell |
| JP2010210772A (ja) | 2009-03-13 | 2010-09-24 | Dainippon Screen Mfg Co Ltd | 液晶表示装置の製造方法 |
| US20130034875A1 (en) | 2009-10-01 | 2013-02-07 | Toto Ltd. | Dna construct, and process for production of recombinant cho cell using same |
| CN105969807A (zh) | 2011-04-05 | 2016-09-28 | 斯克利普斯研究所 | 染色体着陆垫及相关用途 |
| EP2711428A1 (en) | 2012-09-21 | 2014-03-26 | Lonza Biologics plc. | Site-specific integration |
| BR112015031639A2 (pt) | 2013-06-19 | 2019-09-03 | Sigma Aldrich Co Llc | integração alvo |
| JP6708548B2 (ja) | 2013-12-20 | 2020-06-10 | ノバルティス アーゲー | 新規真核細胞および目的の生成物を組換え発現させるための方法 |
| EP3185004A4 (en) | 2014-08-20 | 2018-05-30 | Chugai Seiyaku Kabushiki Kaisha | Method for measuring viscosity of protein solution |
| BR112017008022A2 (pt) * | 2014-10-23 | 2018-02-20 | Regeneron Pharmaceuticals, Inc. | célula, polinucleotídeo, veículo, e, métodos para modificação de um genoma de células cho e para preparação de uma proteína de interesse. |
| CN104818253B (zh) | 2015-03-24 | 2018-03-09 | 成都贝爱特生物科技有限公司 | 靶向定点整合cho细胞系的改造及其用途 |
| DE102015222931A1 (de) * | 2015-11-20 | 2017-05-24 | Continental Teves Ag & Co. Ohg | Anordnung zur Messung eines durch Bremskraft erzeugten Verschiebeweges |
| BR112018071285A2 (pt) | 2016-04-20 | 2019-02-12 | Regeneron Pharma | célula, conjunto de vetores, vetor, sistema, e, método |
| SG11201807885PA (en) | 2016-04-20 | 2018-10-30 | Regeneron Pharma | Compositions and methods for making antibodies based on use of expression-enhancing loci |
| JP2020501593A (ja) | 2016-12-20 | 2020-01-23 | ディベロップメント センター フォー バイオテクノロジーＤｅｖｅｌｏｐｍｅｎｔ Ｃｅｎｔｅｒ Ｆｏｒ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ | 改変細胞、調製方法、及び構築物 |
| CA3068779A1 (en) | 2017-08-11 | 2019-02-14 | Boehringer Ingelheim International Gmbh | Integration sites in cho cells |
-
2020
- 2020-04-01 JP JP2021512163A patent/JP7522100B2/ja active Active
- 2020-04-01 IL IL286630A patent/IL286630B2/en unknown
- 2020-04-01 KR KR1020217034905A patent/KR102655024B1/ko active Active
- 2020-04-01 US US17/600,319 patent/US12344859B2/en active Active
- 2020-04-01 MX MX2021011903A patent/MX2021011903A/es unknown
- 2020-04-01 CA CA3134705A patent/CA3134705A1/en active Pending
- 2020-04-01 TW TW109111209A patent/TWI851703B/zh active
- 2020-04-01 CN CN202080026711.XA patent/CN113661238B/zh active Active
- 2020-04-01 SG SG11202110076PA patent/SG11202110076PA/en unknown
- 2020-04-01 EP EP20784329.3A patent/EP3950938A4/en active Pending
- 2020-04-01 WO PCT/JP2020/014980 patent/WO2020204055A1/ja not_active Ceased
- 2020-04-01 AU AU2020252840A patent/AU2020252840B2/en active Active
Non-Patent Citations (1)
| Title |
|---|
| 期刊 Hamaker, Nathaniel K, and Kelvin H Lee. Site-specific Integration Ushers in a New Era of Precise CHO Cell Line Engineering. Current opinion in chemical engineering vol. 22 Elsevier 2018 152-160 |
Also Published As
| Publication number | Publication date |
|---|---|
| CA3134705A1 (en) | 2020-10-08 |
| IL286630A (en) | 2021-12-01 |
| JPWO2020204055A1 (https=) | 2020-10-08 |
| CN113661238A (zh) | 2021-11-16 |
| AU2020252840B2 (en) | 2025-01-23 |
| BR112021018313A2 (pt) | 2021-11-23 |
| SG11202110076PA (en) | 2021-10-28 |
| KR102655024B1 (ko) | 2024-04-04 |
| TW202043478A (zh) | 2020-12-01 |
| US20220170047A1 (en) | 2022-06-02 |
| US12344859B2 (en) | 2025-07-01 |
| KR20210146362A (ko) | 2021-12-03 |
| CN113661238B (zh) | 2024-08-27 |
| AU2020252840A1 (en) | 2021-11-04 |
| IL286630B1 (en) | 2025-01-01 |
| WO2020204055A1 (ja) | 2020-10-08 |
| EP3950938A1 (en) | 2022-02-09 |
| EP3950938A4 (en) | 2023-01-18 |
| JP7522100B2 (ja) | 2024-07-24 |
| MX2021011903A (es) | 2021-10-26 |
| IL286630B2 (en) | 2025-05-01 |
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