AU2020252840B2 - Method of introducing target-specific foreign gene - Google Patents

Method of introducing target-specific foreign gene Download PDF

Info

Publication number
AU2020252840B2
AU2020252840B2 AU2020252840A AU2020252840A AU2020252840B2 AU 2020252840 B2 AU2020252840 B2 AU 2020252840B2 AU 2020252840 A AU2020252840 A AU 2020252840A AU 2020252840 A AU2020252840 A AU 2020252840A AU 2020252840 B2 AU2020252840 B2 AU 2020252840B2
Authority
AU
Australia
Prior art keywords
exogenous dna
dna
polypeptide
cho cell
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2020252840A
Other languages
English (en)
Other versions
AU2020252840A1 (en
Inventor
Shodai KOMATSU
Kenta KOMURA
Yuuji WAKAHARA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chugai Pharmaceutical Co Ltd
Original Assignee
Chugai Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chugai Pharmaceutical Co Ltd filed Critical Chugai Pharmaceutical Co Ltd
Publication of AU2020252840A1 publication Critical patent/AU2020252840A1/en
Application granted granted Critical
Publication of AU2020252840B2 publication Critical patent/AU2020252840B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2866—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
AU2020252840A 2019-04-02 2020-04-01 Method of introducing target-specific foreign gene Active AU2020252840B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019-070329 2019-04-02
JP2019070329 2019-04-02
PCT/JP2020/014980 WO2020204055A1 (ja) 2019-04-02 2020-04-01 標的特異的な外来遺伝子の導入方法

Publications (2)

Publication Number Publication Date
AU2020252840A1 AU2020252840A1 (en) 2021-11-04
AU2020252840B2 true AU2020252840B2 (en) 2025-01-23

Family

ID=72668649

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2020252840A Active AU2020252840B2 (en) 2019-04-02 2020-04-01 Method of introducing target-specific foreign gene

Country Status (12)

Country Link
US (1) US12344859B2 (https=)
EP (1) EP3950938A4 (https=)
JP (1) JP7522100B2 (https=)
KR (1) KR102655024B1 (https=)
CN (1) CN113661238B (https=)
AU (1) AU2020252840B2 (https=)
CA (1) CA3134705A1 (https=)
IL (1) IL286630B2 (https=)
MX (1) MX2021011903A (https=)
SG (1) SG11202110076PA (https=)
TW (1) TWI851703B (https=)
WO (1) WO2020204055A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025216715A1 (en) * 2024-04-12 2025-10-16 Agency For Science, Technology And Research Identification of transcriptional hotspots in cho cells

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002526071A (ja) * 1998-09-22 2002-08-20 ブリストル−マイヤーズ スクイブ カンパニー トランスフェクションした細胞中の組換えタンパク質発現増大のためのホットスポットを含む発現ベクター
WO2008151219A1 (en) * 2007-06-04 2008-12-11 Regeneron Pharmaceuticals, Inc. Enhanced expression and stability regions

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2707189B1 (fr) 1993-07-09 1995-10-13 Gradient Ass Procédé de traitement de résidus de combustion et installation de mise en Óoeuvre dudit procédé.
US6800457B2 (en) 1998-09-22 2004-10-05 Bristol-Myers Squibb Company Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
DE60021373T2 (de) * 1999-05-10 2006-04-27 Chugai Seiyaku K.K. Verfahren der zellkultivierung
EP1332209B1 (en) 2000-09-08 2009-11-11 Universität Zürich Collections of repeat proteins comprising repeat modules
EP2141243A3 (en) 2000-10-16 2010-01-27 Brystol-Myers Squibb Company Protein scaffolds for antibody mimics and other binding proteins
US20030157561A1 (en) 2001-11-19 2003-08-21 Kolkman Joost A. Combinatorial libraries of monomer domains
WO2003029462A1 (en) 2001-09-27 2003-04-10 Pieris Proteolab Ag Muteins of human neutrophil gelatinase-associated lipocalin and related proteins
US20050164301A1 (en) 2003-10-24 2005-07-28 Avidia Research Institute LDL receptor class A and EGF domain monomers and multimers
CN1618955A (zh) 2003-11-20 2005-05-25 中国人民解放军军事医学科学院生物工程研究所 一种CHO/dhfr－细胞定点整合表达系统
EP1835022B1 (en) * 2005-01-05 2015-02-11 Chugai Seiyaku Kabushiki Kaisha Cell culture method and utilization of the same
JP2007325571A (ja) 2006-06-09 2007-12-20 Toto Ltd 組換え哺乳類動物細胞を用いたタンパク質生産方法
CA2659574C (en) 2006-08-02 2017-04-25 The Uab Research Foundation Methods and compositions related to soluble monoclonal variable lymphocyte receptors of defined antigen specificity
US8071332B2 (en) 2007-08-10 2011-12-06 Toto Ltd. Recombinant mammal cells, method of producing thereof, and method of producing proteins of interest
US8252557B2 (en) * 2008-10-28 2012-08-28 Chugai Seiyaku Kabushiki Kaisha Peptide-containing culture medium for culturing animal cell
JP2010210772A (ja) 2009-03-13 2010-09-24 Dainippon Screen Mfg Co Ltd 液晶表示装置の製造方法
US20130034875A1 (en) 2009-10-01 2013-02-07 Toto Ltd. Dna construct, and process for production of recombinant cho cell using same
CN105969807A (zh) 2011-04-05 2016-09-28 斯克利普斯研究所 染色体着陆垫及相关用途
EP2711428A1 (en) 2012-09-21 2014-03-26 Lonza Biologics plc. Site-specific integration
BR112015031639A2 (pt) 2013-06-19 2019-09-03 Sigma Aldrich Co Llc integração alvo
JP6708548B2 (ja) 2013-12-20 2020-06-10 ノバルティス アーゲー 新規真核細胞および目的の生成物を組換え発現させるための方法
EP3185004A4 (en) 2014-08-20 2018-05-30 Chugai Seiyaku Kabushiki Kaisha Method for measuring viscosity of protein solution
BR112017008022A2 (pt) * 2014-10-23 2018-02-20 Regeneron Pharmaceuticals, Inc. célula, polinucleotídeo, veículo, e, métodos para modificação de um genoma de células cho e para preparação de uma proteína de interesse.
CN104818253B (zh) 2015-03-24 2018-03-09 成都贝爱特生物科技有限公司 靶向定点整合cho细胞系的改造及其用途
DE102015222931A1 (de) * 2015-11-20 2017-05-24 Continental Teves Ag & Co. Ohg Anordnung zur Messung eines durch Bremskraft erzeugten Verschiebeweges
BR112018071285A2 (pt) 2016-04-20 2019-02-12 Regeneron Pharma célula, conjunto de vetores, vetor, sistema, e, método
SG11201807885PA (en) 2016-04-20 2018-10-30 Regeneron Pharma Compositions and methods for making antibodies based on use of expression-enhancing loci
JP2020501593A (ja) 2016-12-20 2020-01-23 ディベロップメント センター フォー バイオテクノロジーＤｅｖｅｌｏｐｍｅｎｔ Ｃｅｎｔｅｒ Ｆｏｒ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 改変細胞、調製方法、及び構築物
CA3068779A1 (en) 2017-08-11 2019-02-14 Boehringer Ingelheim International Gmbh Integration sites in cho cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002526071A (ja) * 1998-09-22 2002-08-20 ブリストル−マイヤーズ スクイブ カンパニー トランスフェクションした細胞中の組換えタンパク質発現増大のためのホットスポットを含む発現ベクター
WO2008151219A1 (en) * 2007-06-04 2008-12-11 Regeneron Pharmaceuticals, Inc. Enhanced expression and stability regions

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NCBI: "JH001259.1 (NW_003614838.1) *

Also Published As

Publication number Publication date
CA3134705A1 (en) 2020-10-08
IL286630A (en) 2021-12-01
JPWO2020204055A1 (https=) 2020-10-08
CN113661238A (zh) 2021-11-16
BR112021018313A2 (pt) 2021-11-23
SG11202110076PA (en) 2021-10-28
KR102655024B1 (ko) 2024-04-04
TW202043478A (zh) 2020-12-01
US20220170047A1 (en) 2022-06-02
US12344859B2 (en) 2025-07-01
KR20210146362A (ko) 2021-12-03
CN113661238B (zh) 2024-08-27
AU2020252840A1 (en) 2021-11-04
IL286630B1 (en) 2025-01-01
WO2020204055A1 (ja) 2020-10-08
TWI851703B (zh) 2024-08-11
EP3950938A1 (en) 2022-02-09
EP3950938A4 (en) 2023-01-18
JP7522100B2 (ja) 2024-07-24
MX2021011903A (es) 2021-10-26
IL286630B2 (en) 2025-05-01

Similar Documents

Publication Publication Date Title
US12503520B2 (en) Compositions and methods for making antibodies based on use of an expression-enhancing locus
CN109715803B (zh) 等位基因编辑及其应用
KR20210076082A (ko) Rna를 편집하기 위한 방법 및 조성물
CN112272704A (zh) 改造的cascade组分和cascade复合体
KR102906454B1 (ko) 발현 강화 유전자좌의 사용에 기초하여 항체를 만들기 위한 조성물 및 방법
JP2012070744A (ja) 哺乳類βアクチンプロモーターを利用した発現系
KR101476010B1 (ko) 고생산성 세포의 수립을 위한 발현 벡터 및 고생산성 세포
AU2020252840B2 (en) Method of introducing target-specific foreign gene
CN112626122A (zh) hKDR人源化小鼠模型及其建立方法和应用
KR101947869B1 (ko) 차세대 시퀀싱 기반 재조합 단백질 발현을 위한 세포 내 핫스팟 영역 탐색 방법
KR20210042084A (ko) Hspa8 유전자의 프로모터
HK40057590A (en) Method of introducing target-specific foreign gene
HK40057590B (zh) 引入靶特异性外源基因的方法
BR112021018313B1 (pt) Métodos para introdução de um dna exógeno codificando um polipeptídeo de interesse dentro de uma célula cho, e para produção de um polipeptídeo
JP4958905B2 (ja) ペプチド鎖発現を増大させるための物質および方法
HK40081918B (en) Methods and compositions for editing rna
EA051643B1 (ru) Композиции и способы получения антител, основанные на применении локуса, обеспечивающего повышенную экспрессию

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)