KR102655024B1 - 표적 특이적인 외래 유전자의 도입 방법 - Google Patents

표적 특이적인 외래 유전자의 도입 방법 Download PDF

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KR102655024B1
KR102655024B1 KR1020217034905A KR20217034905A KR102655024B1 KR 102655024 B1 KR102655024 B1 KR 102655024B1 KR 1020217034905 A KR1020217034905 A KR 1020217034905A KR 20217034905 A KR20217034905 A KR 20217034905A KR 102655024 B1 KR102655024 B1 KR 102655024B1
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dna
cell
cells
gene
polypeptide
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KR20210146362A (ko
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쇼다이 고마쓰
겐타 고무라
유지 와카하라
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추가이 세이야쿠 가부시키가이샤
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2866—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
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  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
KR1020217034905A 2019-04-02 2020-04-01 표적 특이적인 외래 유전자의 도입 방법 Active KR102655024B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019070329 2019-04-02
JPJP-P-2019-070329 2019-04-02
PCT/JP2020/014980 WO2020204055A1 (ja) 2019-04-02 2020-04-01 標的特異的な外来遺伝子の導入方法

Publications (2)

Publication Number Publication Date
KR20210146362A KR20210146362A (ko) 2021-12-03
KR102655024B1 true KR102655024B1 (ko) 2024-04-04

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KR1020217034905A Active KR102655024B1 (ko) 2019-04-02 2020-04-01 표적 특이적인 외래 유전자의 도입 방법

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Country Link
US (1) US12344859B2 (https=)
EP (1) EP3950938A4 (https=)
JP (1) JP7522100B2 (https=)
KR (1) KR102655024B1 (https=)
CN (1) CN113661238B (https=)
AU (1) AU2020252840B2 (https=)
CA (1) CA3134705A1 (https=)
IL (1) IL286630B2 (https=)
MX (1) MX2021011903A (https=)
SG (1) SG11202110076PA (https=)
TW (1) TWI851703B (https=)
WO (1) WO2020204055A1 (https=)

Families Citing this family (1)

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WO2025216715A1 (en) * 2024-04-12 2025-10-16 Agency For Science, Technology And Research Identification of transcriptional hotspots in cho cells

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002526071A (ja) 1998-09-22 2002-08-20 ブリストル−マイヤーズ スクイブ カンパニー トランスフェクションした細胞中の組換えタンパク質発現増大のためのホットスポットを含む発現ベクター
WO2008151219A1 (en) 2007-06-04 2008-12-11 Regeneron Pharmaceuticals, Inc. Enhanced expression and stability regions
US20170002059A1 (en) 2013-12-20 2017-01-05 Thomas Jostock Novel eukaryotic cells and methods for recombinantly expressing a product of interest

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2707189B1 (fr) 1993-07-09 1995-10-13 Gradient Ass Procédé de traitement de résidus de combustion et installation de mise en Óoeuvre dudit procédé.
US6800457B2 (en) 1998-09-22 2004-10-05 Bristol-Myers Squibb Company Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
CN1182244C (zh) * 1999-05-10 2004-12-29 中外制药株式会社 培养细胞的方法
DE60140474D1 (de) 2000-09-08 2009-12-24 Univ Zuerich Sammlung von proteinen mit sich wiederholenden sequenzen (repeat proteins), die repetitive sequenzmodule enthalten
CA2418835A1 (en) 2000-10-16 2002-04-25 Phylos, Inc. Protein scaffolds for antibody mimics and other binding proteins
US20030157561A1 (en) 2001-11-19 2003-08-21 Kolkman Joost A. Combinatorial libraries of monomer domains
WO2003029462A1 (en) 2001-09-27 2003-04-10 Pieris Proteolab Ag Muteins of human neutrophil gelatinase-associated lipocalin and related proteins
WO2005040229A2 (en) 2003-10-24 2005-05-06 Avidia, Inc. Ldl receptor class a and egf domain monomers and multimers
CN1618955A (zh) * 2003-11-20 2005-05-25 中国人民解放军军事医学科学院生物工程研究所 一种CHO/dhfr－细胞定点整合表达系统
JP4818936B2 (ja) * 2005-01-05 2011-11-16 中外製薬株式会社 細胞の培養方法およびその利用
JP2007325571A (ja) 2006-06-09 2007-12-20 Toto Ltd 組換え哺乳類動物細胞を用いたタンパク質生産方法
CA2659574C (en) 2006-08-02 2017-04-25 The Uab Research Foundation Methods and compositions related to soluble monoclonal variable lymphocyte receptors of defined antigen specificity
EP3176263A1 (en) 2007-08-10 2017-06-07 Toto Ltd. Method of producing recombinant mammalian cells
US8252557B2 (en) * 2008-10-28 2012-08-28 Chugai Seiyaku Kabushiki Kaisha Peptide-containing culture medium for culturing animal cell
JP2010210772A (ja) 2009-03-13 2010-09-24 Dainippon Screen Mfg Co Ltd 液晶表示装置の製造方法
US20130034875A1 (en) * 2009-10-01 2013-02-07 Toto Ltd. Dna construct, and process for production of recombinant cho cell using same
AU2012240164A1 (en) 2011-04-05 2013-11-14 The Scripps Research Institute Chromosomal landing pads and related uses
EP2711428A1 (en) 2012-09-21 2014-03-26 Lonza Biologics plc. Site-specific integration
BR112015031639A2 (pt) 2013-06-19 2019-09-03 Sigma Aldrich Co Llc integração alvo
JP6858559B2 (ja) 2014-08-20 2021-04-14 中外製薬株式会社 蛋白質溶液の粘度測定方法
KR102243243B1 (ko) 2014-10-23 2021-04-22 리제너론 파마슈티칼스 인코포레이티드 신규한 cho 통합 부위 및 이의 용도
CN104818253B (zh) * 2015-03-24 2018-03-09 成都贝爱特生物科技有限公司 靶向定点整合cho细胞系的改造及其用途
DE102015222931A1 (de) * 2015-11-20 2017-05-24 Continental Teves Ag & Co. Ohg Anordnung zur Messung eines durch Bremskraft erzeugten Verschiebeweges
EA201892137A1 (ru) 2016-04-20 2019-03-29 Регенерон Фармасьютикалз, Инк. Композиции и способы получения антител, основанные на применении локусов, обеспечивающих повышенную экспрессию
US11512144B2 (en) 2016-04-20 2022-11-29 Regeneran Pharmaceuticals, Inc. Compositions and methods for making antibodies based on use of an expression-enhancing loci
EP4130280A3 (en) 2016-12-20 2023-04-19 Development Center for Biotechnology Targeted integration sites in chinese hamster ovary cell genome
SG11202000966XA (en) * 2017-08-11 2020-02-27 Boehringer Ingelheim Int Integration sites in CHO cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002526071A (ja) 1998-09-22 2002-08-20 ブリストル−マイヤーズ スクイブ カンパニー トランスフェクションした細胞中の組換えタンパク質発現増大のためのホットスポットを含む発現ベクター
WO2008151219A1 (en) 2007-06-04 2008-12-11 Regeneron Pharmaceuticals, Inc. Enhanced expression and stability regions
US20170002059A1 (en) 2013-12-20 2017-01-05 Thomas Jostock Novel eukaryotic cells and methods for recombinantly expressing a product of interest

Also Published As

Publication number Publication date
CN113661238A (zh) 2021-11-16
EP3950938A4 (en) 2023-01-18
IL286630B2 (en) 2025-05-01
US20220170047A1 (en) 2022-06-02
BR112021018313A2 (pt) 2021-11-23
MX2021011903A (es) 2021-10-26
CA3134705A1 (en) 2020-10-08
AU2020252840A1 (en) 2021-11-04
KR20210146362A (ko) 2021-12-03
CN113661238B (zh) 2024-08-27
IL286630A (en) 2021-12-01
TWI851703B (zh) 2024-08-11
US12344859B2 (en) 2025-07-01
SG11202110076PA (en) 2021-10-28
IL286630B1 (en) 2025-01-01
EP3950938A1 (en) 2022-02-09
JPWO2020204055A1 (https=) 2020-10-08
AU2020252840B2 (en) 2025-01-23
WO2020204055A1 (ja) 2020-10-08
JP7522100B2 (ja) 2024-07-24
TW202043478A (zh) 2020-12-01

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