JPS611387A - 組換えｄｎａ発現ベクタ−および遺伝子の発現方法 - Google Patents

組換えｄｎａ発現ベクタ−および遺伝子の発現方法

Info

Publication number
JPS611387A
JPS611387A JP60045684A JP4568485A JPS611387A JP S611387 A JPS611387 A JP S611387A JP 60045684 A JP60045684 A JP 60045684A JP 4568485 A JP4568485 A JP 4568485A JP S611387 A JPS611387 A JP S611387A
Authority
JP
Japan
Prior art keywords
plasmid
sequence
polypeptide
fragment
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP60045684A
Other languages
English (en)
Japanese (ja)
Inventor
ロナルド・ジヨージ・シヨナー
ブリジツト・エリザベス・シヨナー
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Publication of JPS611387A publication Critical patent/JPS611387A/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Diabetes (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
JP60045684A 1984-03-06 1985-03-06 組換えｄｎａ発現ベクタ−および遺伝子の発現方法 Pending JPS611387A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US58659284A 1984-03-06 1984-03-06
US586592 1984-03-06

Publications (1)

Publication Number Publication Date
JPS611387A true JPS611387A (ja) 1986-01-07

Family

ID=24346371

Family Applications (1)

Application Number Title Priority Date Filing Date
JP60045684A Pending JPS611387A (ja) 1984-03-06 1985-03-06 組換えｄｎａ発現ベクタ−および遺伝子の発現方法

Country Status (17)

Country Link
EP (1) EP0154539A3 (enExample)
JP (1) JPS611387A (enExample)
KR (1) KR900001015B1 (enExample)
CN (1) CN85101555A (enExample)
AU (1) AU589355B2 (enExample)
CA (1) CA1283374C (enExample)
DK (1) DK99985A (enExample)
DO (1) DOP1985004337A (enExample)
EG (1) EG17786A (enExample)
ES (3) ES8802322A1 (enExample)
GR (1) GR850571B (enExample)
HU (1) HU202587B (enExample)
IL (1) IL74507A0 (enExample)
NZ (1) NZ211313A (enExample)
PT (1) PT80051B (enExample)
SU (1) SU1491346A3 (enExample)
ZA (1) ZA851626B (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3585170D1 (de) * 1984-03-06 1992-02-27 Lilly Co Eli Expressionsvektoren fuer rinderwachstumshormonderivate.
US5618920A (en) * 1985-11-01 1997-04-08 Xoma Corporation Modular assembly of antibody genes, antibodies prepared thereby and use
US5576195A (en) * 1985-11-01 1996-11-19 Xoma Corporation Vectors with pectate lyase signal sequence
AU582288B2 (en) * 1986-03-07 1989-03-16 Damon Biotech Inc. Vector and method for achieving high level expression in eukaryotic cells
FR2599753B1 (fr) * 1986-06-05 1989-12-08 Sanofi Sa Sequence d'adn composite et son application pour la production de l'hormone de croissance
DE3843894A1 (de) * 1988-12-24 1990-06-28 Behringwerke Ag Plasmide mit translations-start-stop-start codon-konfiguration zur expression von proteinen in e.coli

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2121054B (en) * 1982-05-25 1986-02-26 Lilly Co Eli Cloning vectors for expression of exogenous protein
US4559300A (en) * 1983-01-18 1985-12-17 Eli Lilly And Company Method for using an homologous bacillus promoter and associated natural or modified ribosome binding site-containing DNA sequence in streptomyces
US4713339A (en) * 1983-01-19 1987-12-15 Genentech, Inc. Polycistronic expression vector construction
DK172882B1 (da) * 1983-02-07 1999-09-06 Rothwell Property Ltd DNA indeholdende udtrykkelseskontrolregion fra et eukaryot varmechokproteingen, vektor og eukaryot celle indeholdende DNA'e
IL76422A0 (en) * 1984-09-26 1986-01-31 Lilly Co Eli Method for expression and secretion in bacillus
US4766066A (en) * 1984-09-27 1988-08-23 Eli Lilly And Company Method of using bacteriophage lambda p1 promoter to produce a functional polypeptide in streptomyces

Also Published As

Publication number Publication date
ES8702495A1 (es) 1987-01-01
HU202587B (en) 1991-03-28
CA1283374C (en) 1991-04-23
ES550013A0 (es) 1987-01-01
EP0154539A2 (en) 1985-09-11
SU1491346A3 (ru) 1989-06-30
KR900001015B1 (ko) 1990-02-24
IL74507A0 (en) 1985-06-30
AU589355B2 (en) 1989-10-12
NZ211313A (en) 1988-05-30
DK99985A (da) 1985-09-07
ZA851626B (en) 1986-10-29
EG17786A (en) 1990-10-30
EP0154539A3 (en) 1986-12-30
PT80051B (en) 1987-03-24
DK99985D0 (da) 1985-03-05
ES8706207A1 (es) 1987-06-01
GR850571B (enExample) 1985-07-08
ES8802322A1 (es) 1988-05-01
ES550012A0 (es) 1987-06-01
DOP1985004337A (es) 1990-09-17
KR850006703A (ko) 1985-10-16
CN85101555A (zh) 1987-01-24
ES540936A0 (es) 1988-05-01
AU3950285A (en) 1985-09-12
HUT40162A (en) 1986-11-28
PT80051A (en) 1985-04-01

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