ES8802322A1 - Un procedimiento para la preparacion de un plasmido de expresion de dna recombinante. - Google Patents
Un procedimiento para la preparacion de un plasmido de expresion de dna recombinante.Info
- Publication number
- ES8802322A1 ES8802322A1 ES540936A ES540936A ES8802322A1 ES 8802322 A1 ES8802322 A1 ES 8802322A1 ES 540936 A ES540936 A ES 540936A ES 540936 A ES540936 A ES 540936A ES 8802322 A1 ES8802322 A1 ES 8802322A1
- Authority
- ES
- Spain
- Prior art keywords
- recombinant dna
- translational
- expression vectors
- codes
- reading frame
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 108020004511 Recombinant DNA Proteins 0.000 title abstract 2
- 239000013604 expression vector Substances 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 230000014509 gene expression Effects 0.000 title 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 3
- 239000002773 nucleotide Substances 0.000 abstract 2
- 125000003729 nucleotide group Chemical group 0.000 abstract 2
- 229920001184 polypeptide Polymers 0.000 abstract 2
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 230000003213 activating effect Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 108020004999 messenger RNA Proteins 0.000 abstract 1
- 230000003362 replicative effect Effects 0.000 abstract 1
- 238000013518 transcription Methods 0.000 abstract 1
- 230000035897 transcription Effects 0.000 abstract 1
- 230000002103 transcriptional effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/69—Increasing the copy number of the vector
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Endocrinology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Diabetes (AREA)
- Cell Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
PROCEDIMIENTO DE OBTENCION PARA UN VECTOR DE EXPRESION DE DNA RECOMBINANTE QUE CONSISTE EN LIGAR: A) UNA SECUENCIA ACTIVANTE TRANSCRIPCIONAL Y TRADUCCIONAL EN LA ESTRUCTURA LECTORA DE UNA SECUENCIA DE UN NUCLEOTIDO QUE CODIFICA UN PEPTIDO O POLIPEPTIDO Y QUE CONTIENE UNA SEÑAL DE PARADA, ADYACENTE AL TRIPLETE QUE CODIFICA EL CARBOXI TERMINAL; Y B) UNA SEÑAL DE COMIENZO ADYACENTE A LA SEÑAL DE PARADA EN LA ESTRUCTURA LECTORA DE LA SECUENCIA DEL NUCLEOTIDO. EL VECTOR ES SELECCIONABLE Y AUTONOMAMENTE REPLICANTE Y SE GENERA EN LA TRANSCRIPCION DEL POLICISTRONICO RNAM TRADUCIBLE. SE RESUELVEN ASI, LOS PROBLEMAS DE EXPRESION, SIN LA NECESIDAD DE LA MODIFICACION SINTETICA DE UN GEN, YA QUE SE ALTERA EL EXTREMO-5K DE UN RNAM TRANSCRITO. LAS SECUENCIAS CODIFICAN ESPECIALMENTE, HORMONAS DEL CRECIMIENTO, CADENAS DE INSULINA HUMANA, INTERFERON, ACTIVADORES DE PLASMINOGENO EN EL TEJIDOHUMANO, ETC.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US58659284A | 1984-03-06 | 1984-03-06 |
Publications (2)
Publication Number | Publication Date |
---|---|
ES540936A0 ES540936A0 (es) | 1988-05-01 |
ES8802322A1 true ES8802322A1 (es) | 1988-05-01 |
Family
ID=24346371
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES540936A Expired ES8802322A1 (es) | 1984-03-06 | 1985-03-05 | Un procedimiento para la preparacion de un plasmido de expresion de dna recombinante. |
ES550013A Expired ES8702495A1 (es) | 1984-03-06 | 1985-12-16 | Un metodo de produccion de un polipeptido funcional |
ES550012A Expired ES8706207A1 (es) | 1984-03-06 | 1985-12-16 | Un procedimiento para la preparacion del plasmido pcz101 |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES550013A Expired ES8702495A1 (es) | 1984-03-06 | 1985-12-16 | Un metodo de produccion de un polipeptido funcional |
ES550012A Expired ES8706207A1 (es) | 1984-03-06 | 1985-12-16 | Un procedimiento para la preparacion del plasmido pcz101 |
Country Status (17)
Country | Link |
---|---|
EP (1) | EP0154539A3 (es) |
JP (1) | JPS611387A (es) |
KR (1) | KR900001015B1 (es) |
CN (1) | CN85101555A (es) |
AU (1) | AU589355B2 (es) |
CA (1) | CA1283374C (es) |
DK (1) | DK99985A (es) |
DO (1) | DOP1985004337A (es) |
EG (1) | EG17786A (es) |
ES (3) | ES8802322A1 (es) |
GR (1) | GR850571B (es) |
HU (1) | HU202587B (es) |
IL (1) | IL74507A0 (es) |
NZ (1) | NZ211313A (es) |
PT (1) | PT80051B (es) |
SU (1) | SU1491346A3 (es) |
ZA (1) | ZA851626B (es) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3585170D1 (de) * | 1984-03-06 | 1992-02-27 | Lilly Co Eli | Expressionsvektoren fuer rinderwachstumshormonderivate. |
US5618920A (en) * | 1985-11-01 | 1997-04-08 | Xoma Corporation | Modular assembly of antibody genes, antibodies prepared thereby and use |
US5576195A (en) * | 1985-11-01 | 1996-11-19 | Xoma Corporation | Vectors with pectate lyase signal sequence |
AU582288B2 (en) * | 1986-03-07 | 1989-03-16 | Damon Biotech Inc. | Vector and method for achieving high level expression in eukaryotic cells |
FR2599753B1 (fr) * | 1986-06-05 | 1989-12-08 | Sanofi Sa | Sequence d'adn composite et son application pour la production de l'hormone de croissance |
DE3843894A1 (de) * | 1988-12-24 | 1990-06-28 | Behringwerke Ag | Plasmide mit translations-start-stop-start codon-konfiguration zur expression von proteinen in e.coli |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3380262D1 (en) * | 1982-05-25 | 1989-08-31 | Lilly Co Eli | Cloning vectors for expression of exogenous protein |
US4559300A (en) * | 1983-01-18 | 1985-12-17 | Eli Lilly And Company | Method for using an homologous bacillus promoter and associated natural or modified ribosome binding site-containing DNA sequence in streptomyces |
US4713339A (en) * | 1983-01-19 | 1987-12-15 | Genentech, Inc. | Polycistronic expression vector construction |
NO172595C (no) * | 1983-02-07 | 1993-08-11 | Battelle Memorial Institute | Fremgangsmaate og ekspresjonsplasmid for fremstilling av hemagglutinin under kontroll av drosophila hsp-promoter |
EP0176320B1 (en) * | 1984-09-26 | 1990-07-25 | Eli Lilly And Company | A method for expression and secretion in bacillus |
US4766066A (en) * | 1984-09-27 | 1988-08-23 | Eli Lilly And Company | Method of using bacteriophage lambda p1 promoter to produce a functional polypeptide in streptomyces |
-
1985
- 1985-03-04 DO DO1985004337A patent/DOP1985004337A/es unknown
- 1985-03-04 ZA ZA851626A patent/ZA851626B/xx unknown
- 1985-03-04 SU SU853862498A patent/SU1491346A3/ru active
- 1985-03-04 EP EP85301469A patent/EP0154539A3/en not_active Ceased
- 1985-03-05 ES ES540936A patent/ES8802322A1/es not_active Expired
- 1985-03-05 PT PT80051A patent/PT80051B/pt not_active IP Right Cessation
- 1985-03-05 CA CA000475732A patent/CA1283374C/en not_active Expired - Lifetime
- 1985-03-05 IL IL74507A patent/IL74507A0/xx unknown
- 1985-03-05 HU HU85836A patent/HU202587B/hu not_active IP Right Cessation
- 1985-03-05 AU AU39502/85A patent/AU589355B2/en not_active Ceased
- 1985-03-05 DK DK99985A patent/DK99985A/da not_active Application Discontinuation
- 1985-03-05 NZ NZ211313A patent/NZ211313A/en unknown
- 1985-03-06 EG EG140/85A patent/EG17786A/xx active
- 1985-03-06 KR KR1019850001420A patent/KR900001015B1/ko not_active IP Right Cessation
- 1985-03-06 GR GR850571A patent/GR850571B/el unknown
- 1985-03-06 JP JP60045684A patent/JPS611387A/ja active Pending
- 1985-04-01 CN CN198585101555A patent/CN85101555A/zh active Pending
- 1985-12-16 ES ES550013A patent/ES8702495A1/es not_active Expired
- 1985-12-16 ES ES550012A patent/ES8706207A1/es not_active Expired
Also Published As
Publication number | Publication date |
---|---|
IL74507A0 (en) | 1985-06-30 |
ES550013A0 (es) | 1987-01-01 |
DK99985A (da) | 1985-09-07 |
KR850006703A (ko) | 1985-10-16 |
ES540936A0 (es) | 1988-05-01 |
CA1283374C (en) | 1991-04-23 |
SU1491346A3 (ru) | 1989-06-30 |
GR850571B (es) | 1985-07-08 |
JPS611387A (ja) | 1986-01-07 |
CN85101555A (zh) | 1987-01-24 |
EP0154539A2 (en) | 1985-09-11 |
AU589355B2 (en) | 1989-10-12 |
HUT40162A (en) | 1986-11-28 |
PT80051A (en) | 1985-04-01 |
EP0154539A3 (en) | 1986-12-30 |
ES550012A0 (es) | 1987-06-01 |
AU3950285A (en) | 1985-09-12 |
DOP1985004337A (es) | 1990-09-17 |
KR900001015B1 (ko) | 1990-02-24 |
HU202587B (en) | 1991-03-28 |
ES8706207A1 (es) | 1987-06-01 |
DK99985D0 (da) | 1985-03-05 |
NZ211313A (en) | 1988-05-30 |
PT80051B (en) | 1987-03-24 |
EG17786A (en) | 1990-10-30 |
ZA851626B (en) | 1986-10-29 |
ES8702495A1 (es) | 1987-01-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
MM4A | Patent lapsed |
Effective date: 19960401 |