ES540936A0 - Un procedimiento para la preparacion de un plasmido de expresion de dna recombinante. - Google Patents

Un procedimiento para la preparacion de un plasmido de expresion de dna recombinante.

Info

Publication number
ES540936A0
ES540936A0 ES540936A ES540936A ES540936A0 ES 540936 A0 ES540936 A0 ES 540936A0 ES 540936 A ES540936 A ES 540936A ES 540936 A ES540936 A ES 540936A ES 540936 A0 ES540936 A0 ES 540936A0
Authority
ES
Spain
Prior art keywords
procedure
preparation
recombinant dna
dna expression
plasmide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES540936A
Other languages
English (en)
Other versions
ES8802322A1 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Publication of ES8802322A1 publication Critical patent/ES8802322A1/es
Publication of ES540936A0 publication Critical patent/ES540936A0/es
Expired legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • C12N15/69Increasing the copy number of the vector
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • C07K14/61Growth hormones [GH] (Somatotropin)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • C07K14/62Insulins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Endocrinology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
ES540936A 1984-03-06 1985-03-05 Un procedimiento para la preparacion de un plasmido de expresion de dna recombinante. Expired ES8802322A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US58659284A 1984-03-06 1984-03-06

Publications (2)

Publication Number Publication Date
ES8802322A1 ES8802322A1 (es) 1988-05-01
ES540936A0 true ES540936A0 (es) 1988-05-01

Family

ID=24346371

Family Applications (3)

Application Number Title Priority Date Filing Date
ES540936A Expired ES8802322A1 (es) 1984-03-06 1985-03-05 Un procedimiento para la preparacion de un plasmido de expresion de dna recombinante.
ES550013A Expired ES8702495A1 (es) 1984-03-06 1985-12-16 Un metodo de produccion de un polipeptido funcional
ES550012A Expired ES8706207A1 (es) 1984-03-06 1985-12-16 Un procedimiento para la preparacion del plasmido pcz101

Family Applications After (2)

Application Number Title Priority Date Filing Date
ES550013A Expired ES8702495A1 (es) 1984-03-06 1985-12-16 Un metodo de produccion de un polipeptido funcional
ES550012A Expired ES8706207A1 (es) 1984-03-06 1985-12-16 Un procedimiento para la preparacion del plasmido pcz101

Country Status (17)

Country Link
EP (1) EP0154539A3 (es)
JP (1) JPS611387A (es)
KR (1) KR900001015B1 (es)
CN (1) CN85101555A (es)
AU (1) AU589355B2 (es)
CA (1) CA1283374C (es)
DK (1) DK99985A (es)
DO (1) DOP1985004337A (es)
EG (1) EG17786A (es)
ES (3) ES8802322A1 (es)
GR (1) GR850571B (es)
HU (1) HU202587B (es)
IL (1) IL74507A0 (es)
NZ (1) NZ211313A (es)
PT (1) PT80051B (es)
SU (1) SU1491346A3 (es)
ZA (1) ZA851626B (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0159123B1 (en) * 1984-03-06 1992-01-15 Eli Lilly And Company Vectors for expressing bovine growth hormone derivatives
US5618920A (en) * 1985-11-01 1997-04-08 Xoma Corporation Modular assembly of antibody genes, antibodies prepared thereby and use
US5576195A (en) * 1985-11-01 1996-11-19 Xoma Corporation Vectors with pectate lyase signal sequence
AU582288B2 (en) * 1986-03-07 1989-03-16 Damon Biotech Inc. Vector and method for achieving high level expression in eukaryotic cells
FR2599753B1 (fr) * 1986-06-05 1989-12-08 Sanofi Sa Sequence d'adn composite et son application pour la production de l'hormone de croissance
DE3843894A1 (de) * 1988-12-24 1990-06-28 Behringwerke Ag Plasmide mit translations-start-stop-start codon-konfiguration zur expression von proteinen in e.coli

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE55244B1 (en) * 1982-05-25 1990-07-18 Lilly Co Eli Cloning vectors for expression of exogenous protein
US4559300A (en) * 1983-01-18 1985-12-17 Eli Lilly And Company Method for using an homologous bacillus promoter and associated natural or modified ribosome binding site-containing DNA sequence in streptomyces
US4713339A (en) * 1983-01-19 1987-12-15 Genentech, Inc. Polycistronic expression vector construction
NO172595C (no) * 1983-02-07 1993-08-11 Battelle Memorial Institute Fremgangsmaate og ekspresjonsplasmid for fremstilling av hemagglutinin under kontroll av drosophila hsp-promoter
EP0176320B1 (en) * 1984-09-26 1990-07-25 Eli Lilly And Company A method for expression and secretion in bacillus
US4766066A (en) * 1984-09-27 1988-08-23 Eli Lilly And Company Method of using bacteriophage lambda p1 promoter to produce a functional polypeptide in streptomyces

Also Published As

Publication number Publication date
ES550013A0 (es) 1987-01-01
SU1491346A3 (ru) 1989-06-30
CN85101555A (zh) 1987-01-24
ES8802322A1 (es) 1988-05-01
DK99985D0 (da) 1985-03-05
ZA851626B (en) 1986-10-29
HUT40162A (en) 1986-11-28
PT80051B (en) 1987-03-24
IL74507A0 (en) 1985-06-30
CA1283374C (en) 1991-04-23
ES8702495A1 (es) 1987-01-01
EP0154539A2 (en) 1985-09-11
DK99985A (da) 1985-09-07
ES550012A0 (es) 1987-06-01
GR850571B (es) 1985-07-08
PT80051A (en) 1985-04-01
JPS611387A (ja) 1986-01-07
NZ211313A (en) 1988-05-30
HU202587B (en) 1991-03-28
EG17786A (en) 1990-10-30
AU589355B2 (en) 1989-10-12
EP0154539A3 (en) 1986-12-30
KR900001015B1 (ko) 1990-02-24
DOP1985004337A (es) 1990-09-17
ES8706207A1 (es) 1987-06-01
KR850006703A (ko) 1985-10-16
AU3950285A (en) 1985-09-12

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Legal Events

Date Code Title Description
MM4A Patent lapsed

Effective date: 19960401