JPH09504534A - C型肝炎ウイルスの免疫優性ヒトt細胞エピトープ - Google Patents
C型肝炎ウイルスの免疫優性ヒトt細胞エピトープInfo
- Publication number
- JPH09504534A JPH09504534A JP7513004A JP51300495A JPH09504534A JP H09504534 A JPH09504534 A JP H09504534A JP 7513004 A JP7513004 A JP 7513004A JP 51300495 A JP51300495 A JP 51300495A JP H09504534 A JPH09504534 A JP H09504534A
- Authority
- JP
- Japan
- Prior art keywords
- hcv
- polypeptide
- amino acids
- region
- peptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.HCVの、コア領域のアミノ酸73〜176の間、またはE1領域のアミノ 酸192〜234と243〜392の間、またはE2領域のアミノ酸397およ び428およびアミノ酸571〜638の間、またはNS3領域のアミノ酸11 88および1463の間に含まれる領域から選択される少なくとも8個の連続す るアミノ酸を含むかまたはそれよりなり、上記連続するアミノ酸が、T細胞刺激 性エピトープを含む、約8個〜約100個のアミノ酸のポリペプチドの、HCV 免疫原性組成物の調製のための用途。 2.ポリペプチドが、以下の配列: 〔式中、X1はRまたはKを表し、X2はA、SまたはTを表し、X3はAまたは Gを表し、X4はQ、KまたはRを表し、X5はYまたはHを表し、X6はGまた はAを表し、X7はEまたはKを表し、X8はC、MまたはLを表し、X9はWま たはLを表し、X10はS、N、T、DまたはHを表し、X11はPまたはQを表し 、X12はNまたはTを表し、X13はRまたはHを表し、X14はRまたはKを表し 、X14はLまたはVまたはFを表し、X16はKまたはRを表し、X17はLまたは Iを表し、X18はFまたはLを表し、X19はMまたはIを表し、X20はGまたは Eを表し、X21はLまたはVまたはIを表し、X22はVまたはLを表し、X23は AまたはGを表し、X24はL、VまたはIを表し、X25はAまたはVを表し、X26 はAまたはSを表し、X27はRまたはAを表し、X28はAまたはTまたは Eを表し、X29はAまたはEを表し、X30はVまたはAまたはLを表し、X31は LまたはVまたはIを表し、X32はEまたはGを表し、X33はVまたはIを表し 、X34はFまたはYを表し、X35はAまたはPを表し、X36はLまたはIを表す ]、および を特徴とする、アミノ酸73〜176の間に含まれる領域から選択される約8個 〜約104個の連続するアミノ酸を含むかまたはそれよりなる、より詳細にはH CVのコア領域のアミノ酸109〜176の間の領域から選択される約8個〜約 68個の連続するアミノ酸を含むかまたはそれよりなり、上記連続するアミノ酸 はT細胞刺激性エピトープを含む、HCV免疫原性組成物の調製のための請求項 1記載のポリペプチドの用途。 3.ポリペプチドが、以下の配列: または図4に示す別のタイプのHCV由来のこの配列のあらゆる変異体を特徴と する、HCVのアミノ酸192〜234と243〜392の間に含まれる領域か ら選択される、より詳細にはHCVのE1領域のアミノ酸192および234お よび243〜383の間に含まれる領域から選択される、少なくとも約8個の連 続するアミノ酸を含むかまたはそれよりなり、上記連続するアミノ酸はT細胞刺 激性エピトープを含む、HCV免疫 原性組成物の調製のための請求項1記載のポリペプチドの用途。 4.ポリペプチドが、以下の配列: 〔式中、X37はS、A、Q、L、N、Y、R、YまたはHを表し、X38はG、S 、T、AまたはRを表し、X39はF、I、LまたはVを表し、X40はV、Aまた はTを表し、X41はS、DまたはGを表し、X42はL、I、W、FまたはMを表 し、X43はL、IまたはFを表し、X44はA、T、DまたはSを表し、X45はP 、Q.S、R、L、IまたはTを表し、X46はA、PまたはSを表し、X47はK 、S、Q、AまたはRを表し、X48はN、K、DまたはRを表し、X49はV、I またはLを表し、X50はQ、SまたはYを表し、X51はIまたはVを表し、X52 はLまたはIを表し、X53はSまたはRを表し、X54はTまたはSを表し、X55 はGまたはRを表し、X56はG、AまたはKを表し、X57はA、V、G、Sまた はDを表し、X58はG、FまたはYを表し、X59はN、H、R、L、AまたはS を表し、X60はTまたはSを表し、Z1はMまたはIを表し、Z2はDを表し、X61 はH、L、V、TまたはIを表し、X62はHまたはYを表し、X63はDまたは Eを表し、X64はAまたはTを表し、X65はS、T、IまたはLを表し、X66は RまたはKを表し、X67はSまたはAを表し、X68はWまたはLを表し、X69は IまたはLを表し、X70はL、MまたはIを表し、X71はVまたはIを表し、X72 はI、V、FまたはLを表し、X73はYまたはFを表し、X74はT、Sまたは Aを表し、X75はIまたはVを 表し、X76はI、VまたはAを表し、X77はYまたはFを表す。〕 を特徴とする、HCVの、E2領域のアミノ酸397〜428の間の領域から選 択される約8個〜約32個の連続するアミノ酸を含むかまたはそれよりなる、ま たはE2領域のアミノ酸571〜638の間の領域から選択される少なくとも8 個〜約68個の連続するアミノ酸を含むかまたはそれよりなり、上記連続するア ミノ酸はT細胞刺激性エピトープを含む、HCV免疫原性組成物の調製のための 請求項1記載のポリペプチドの用途。 5.ポリペプチドが、以下の配列: を特徴とするHCVのNS3領域のアミノ酸1188位〜1463位の間に含ま れる領域または図6から演繹されうる上記の配列のあらゆる変異体から選択され る、少なくとも約8個の連続するアミノ酸を含むかまたはそれよりなり、上記連 続するアミノ酸はT細胞刺激性エピトープを含む、HCV免疫原性組成物の調製 のための請求項1記載のポリペプチドの用途。 6.ポリペプチドが、HCVのコア領域のアミノ酸157位〜176位の間に含 まれる領域: から選択される、約8個〜約20個の連続するアミノ酸を含むかまたはそれより なるポリペプチドであり、好ましくは以下のペプチドのリスト: そしてより詳細には: から選択されるペプチドであり、上記連続するアミノ酸はT細胞刺激性エピトー プを含む、HCV免疫原性組成物の調製のための請求項2記載のポリペプチドの 用途。 7.ポリペプチドが、HCVのコア領域のアミノ酸145位〜164位の間に含 まれる領域: から選択される、約8個〜約20個の連続するアミノ酸を含むかまたはそ れよりなるポリペプチドであり、特に以下のペプチドのリスト: そしてより詳細には: から選択されるペプチドであり、上記連続するアミノ酸はT細胞刺激性エピトー プを含む、HCV免疫原性組成物の調製のための請求項2記載のポリペプチドの 用途。 8.ポリペプチドが、HCVのコア領域のアミノ酸133位〜152位の間に含 まれる領域: 約20個の連続するアミノ酸を含むかまたはそれよりなるポリペプチドであり、 好ましくは以下のペプチドのリスト: そしてより詳細には: から選択されるペプチドであり、上記連続するアミノ酸はT細胞刺激性エピトー プを含む、HCV免疫原性組成物の調製のための請求項2記載のポリペプチドの 用途。 9.ポリペプチドが、HCVのコア領域のアミノ酸109位〜128位の間に含 まれる領域: 個の連続するアミノ酸を含むかまたはそれよりなるポリペプチドであり、特に以 下のペプチド: から選択されるペプチドであり、上記連続するアミノ酸はT細胞刺激性エピトー プを含む、HCV免疫原性組成物の調製のための請求項2記載のポリペプチドの 用途。 10.ポリペプチドが、HCVのコア領域のアミノ酸73位〜92位の間に含まれ る領域: から選択される、より詳細には から選択される、約8個〜約20個の連続するアミノ酸を含むかまたはそれより なるポリペプチドであり、好ましくは以下のペプチド: から選択されるペプチド、例えば、ペプチド: であり、上記連続するアミノ酸はT細胞刺激性エピトープを含む、HCV免疫原 性組成物の調製のための請求項2記載のポリペプチドの用途。 11.ポリペプチドが、HCVのコア領域のアミノ酸133位〜176位の間に含 まれる領域: アミノ酸を含むかまたはそれよりなるポリペプチドであり、上記連続するアミノ 酸はT細胞刺激性エピトープを含む、HCV免疫原性組成物の調製のための請求 項2記載のポリペプチドの用途。 12.ポリペプチドが、以下の配列: を特徴とする、HCVのE1領域のアミノ酸193位〜234位および243位 〜260位の間に含まれる領域から選択される、特に: または図4に示すHCVの別のタイプ由来のこれらの配列のあらゆる変異体から 選択される、少なくとも8個〜約68個の連続するアミノ酸を含むかまたはそれ よりなるポリペプチドであり、上記連続するアミノ酸はT細胞刺激性エピトープ を含む、HCV免疫原性組成物の調製のための請求項3記載のポリペプチドの用 途。 13.ポリペプチドが、以下の配列: を特徴とするHCVのE1領域のアミノ酸253〜332の間に含まれる領域、 または図4に示すHCVの別のタイプ由来のこの配列のあらゆる変異体から選択 される、少なくとも8個〜約80個の連続するアミノ酸を含むかまたはそれより なるポリペプチドであり、特に: から選択されるペプチドであり、上記連続するアミノ酸はT細胞刺激性エピトー プを含む、HCV免疫原性組成物の調製のための請求項3記載のポリペプチドの 用途。 14.ポリペプチドが、以下の配列: を特徴とするHCVのE1領域のアミノ酸325〜392の間に含まれる領域、 または図4に示すHCVの別のタイプ由来のこの配列のあらゆる変異体から選択 される、少なくとも8個〜約68個の連続するアミノ酸を含むかまたはそれより なるポリペプチドであり、特に: から選択されるペプチドであり、上記連続するアミノ酸はT細胞刺激性エピトー プを含む、HCV免疫原性組成物の調製のための請求項3記載のポリペプチドの 用途。 15.ポリペプチドが、HCVのE2領域のアミノ酸397位〜416位の間に含 まれる領域: 約20個の連続するアミノ酸を含むかまたはそれよりなるポリペプチドであり、 上記連続するアミノ酸はT細胞刺激性エピトープを含む、HCV免疫原性組成物 の調製のための請求項4記載のポリペプチドの用途。 16.ポリペプチドが、HCVのE2領域のアミノ酸409位〜428位の間に含 まれる領域: 約20個の連続するアミノ酸を含むかまたはそれよりなるポリペプチドであり、 特に: そしてより詳細には: から選択されるペプチドであり、上記連続するアミノ酸はT細胞刺激性エピトー プを含む、HCV免疫原性組成物の調製のための請求項4記載のポリペプチドの 用途。 17.ポリペプチドが、HCVのE2領域のアミノ酸571位〜638位の間に含 まれる領域: スト: から選択される、少なくとも8個〜約20個の連続するアミノ酸を含むかまたは それよりなるポリペプチドであり、上記連続するアミノ酸はT細胞刺激性エピト ープを含む、HCV免疫原性組成物の調製のための請求項4記載のポリペプチド の用途。 18.ポリペプチドが、HCVのNS3領域のアミノ酸1188位〜1463位の 間に含まれる領域から選択される少なくとも8個の連続するアミノ酸を含むかま たはそれよりなるポリペプチドであり、上記ペプチドが以下のペプチド: または図6から演繹されうる上記配列のあらゆる変異体から選択されるペプチド であり、上記連続するアミノ酸はT細胞刺激性エピトープを含む、 HCV免疫原性組成物の調製のための請求項5記載のポリペプチドの用途。 19.上記T細胞刺激性エピトープが、ヘルパーT細胞エピトープである、請求項 1〜18のいずれか1項記載のポリペプチドの用途。 20.上記T細胞刺激性エピトープが、CTLエピトープである、請求項1〜18 のいずれか1項記載のポリペプチドの用途。 21.予防ワクチン組成物への取り込みのための請求項1〜20のいずれか1項記 載のポリペプチドの用途。 22.治療ワクチン組成物への取り込みのための請求項1〜20のいずれか1項記 載のポリペプチドの用途。 23.請求項1〜22のいずれか1項記載のT細胞刺激性エピトープを含むよう選 択された連続するアミノ酸配列のいずれかの、複数のリピート、組み合わせまた はミモトープを、そのアミノ酸配列中に含むポリペプチド。 24.請求項1〜23のいずれか1項記載のポリペプチドをコードする核酸インサ ートを含む発現ベクターにより発現された組換えポリペプチドである、請求項1 〜23のいずれか1項記載のポリペプチド。 25.HCVエンベロープタンパク質E1およびE2、またはHCVのNS3、N S4またはNS5免疫原、またはB細胞刺激性エピトープを含むHCVペプチド のような病原因子関連免疫原に作動可能に連結されている、請求項1〜24のい ずれか1項記載のポリペプチド。 26.T細胞エピトープを含む、以下のペプチド: のいずれかの配列を含むかまたはそれよりなるペプチド。 27.医薬的に許容可能な賦形剤と混合された請求項26記載のポリペプチドまた はペプチドの少なくとも1つを含むかまたはそれよりなる免疫原性組成物。 28.ワクチン組成物である、請求項27記載の組成物。 29.予防ワクチン組成物である、請求項28記載の組成物。 30.治療ワクチン組成物である、請求項28記載の組成物。 31.請求項26記載のいずれかのポリペプチドに加えて、少なくとも1つのHC VのB細胞刺激性エピトープを含むペプチドまたはポリペプチド、および/また はHCV構造ポリペプチド、および/またはHCV非構造ポリペプチドを含む、 請求項27〜30のいずれか1項記載の組成物。 32.請求項26記載の上記ポリペプチドが、HBsAgまたはHBcAg粒子、 HBV免疫原、HIV免疫原性および/またはHTLV免疫原と混合されている 、請求項27〜31のいずれか1項記載の組成物。
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ES (2) | ES2197170T3 (ja) |
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WO (1) | WO1995012677A2 (ja) |
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1994
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- 1994-10-28 DK DK94931000T patent/DK0725824T3/da active
- 1994-10-28 US US08/635,886 patent/US6555114B1/en not_active Expired - Fee Related
- 1994-10-28 DE DE69432475T patent/DE69432475T2/de not_active Revoked
- 1994-10-28 PT PT94931000T patent/PT725824E/pt unknown
- 1994-10-28 AT AT99116672T patent/ATE274578T1/de not_active IP Right Cessation
- 1994-10-28 DE DE69434295T patent/DE69434295T2/de not_active Revoked
- 1994-10-28 AU AU79932/94A patent/AU698878B2/en not_active Ceased
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- 1994-10-28 ES ES94931000T patent/ES2197170T3/es not_active Expired - Lifetime
- 1994-10-28 WO PCT/EP1994/003555 patent/WO1995012677A2/en not_active Application Discontinuation
- 1994-10-28 DE DE69433971T patent/DE69433971T2/de not_active Expired - Fee Related
- 1994-10-28 ES ES99116671T patent/ES2239819T3/es not_active Expired - Lifetime
- 1994-10-28 PT PT99116671T patent/PT992580E/pt unknown
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- 1994-10-28 DK DK99116671T patent/DK0992580T3/da active
- 1994-10-28 EP EP99116673A patent/EP0979867A3/en not_active Withdrawn
- 1994-10-28 EP EP99116671A patent/EP0992580B1/en not_active Revoked
- 1994-10-28 JP JP7513004A patent/JPH09504534A/ja active Pending
- 1994-10-28 CA CA002175692A patent/CA2175692A1/en not_active Abandoned
- 1994-10-28 AT AT94931000T patent/ATE236981T1/de not_active IP Right Cessation
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2003
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2003064096A (ja) * | 2001-08-29 | 2003-03-05 | Mitsubishi Kagaku Bio-Clinical Laboratories Inc | C型肝炎ウイルス特異的細胞障害性t細胞認識エピトープ |
JP2008508890A (ja) * | 2004-08-11 | 2008-03-27 | セントロ デ インジエニエリア ジエネテイカ イ バイオテクノロジア | 組換え生鶏痘ウィルスベクター及びc型肝炎ウィルスに対する薬剤組成物中でのそれらの使用 |
WO2012033142A1 (ja) * | 2010-09-08 | 2012-03-15 | 学校法人 埼玉医科大学 | C型肝炎ウイルスリポソームワクチン |
US9814785B2 (en) | 2010-09-08 | 2017-11-14 | Saitama Medical University | Hepatitis C virus liposome vaccine |
JP2016512242A (ja) * | 2013-03-14 | 2016-04-25 | アボット・ラボラトリーズAbbott Laboratories | Hcvコア脂質結合ドメインモノクローナル抗体 |
JP2019081764A (ja) * | 2013-03-14 | 2019-05-30 | アボット・ラボラトリーズAbbott Laboratories | Hcvコア脂質結合ドメインモノクローナル抗体 |
JP2022518488A (ja) * | 2019-01-25 | 2022-03-15 | センティ バイオサイエンシズ インコーポレイテッド | タンパク質機能を制御するための融合構築物 |
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