JPH0573390B2 - - Google Patents
Info
- Publication number
- JPH0573390B2 JPH0573390B2 JP62075907A JP7590787A JPH0573390B2 JP H0573390 B2 JPH0573390 B2 JP H0573390B2 JP 62075907 A JP62075907 A JP 62075907A JP 7590787 A JP7590787 A JP 7590787A JP H0573390 B2 JPH0573390 B2 JP H0573390B2
- Authority
- JP
- Japan
- Prior art keywords
- phospholipase
- carbon atoms
- phosphatidyl group
- phospholipids
- phosphatidic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 36
- 102000011420 Phospholipase D Human genes 0.000 claims description 33
- 108090000553 Phospholipase D Proteins 0.000 claims description 33
- 125000001095 phosphatidyl group Chemical group 0.000 claims description 27
- 150000003904 phospholipids Chemical class 0.000 claims description 24
- 229910021645 metal ion Inorganic materials 0.000 claims description 17
- 238000006276 transfer reaction Methods 0.000 claims description 17
- 125000004432 carbon atom Chemical group C* 0.000 claims description 16
- 238000004519 manufacturing process Methods 0.000 claims description 16
- 230000000694 effects Effects 0.000 claims description 15
- 150000008103 phosphatidic acids Chemical class 0.000 claims description 12
- RMVRSNDYEFQCLF-UHFFFAOYSA-N thiophenol Chemical compound SC1=CC=CC=C1 RMVRSNDYEFQCLF-UHFFFAOYSA-N 0.000 claims description 9
- -1 aliphatic ethers Chemical class 0.000 claims description 7
- 230000003213 activating effect Effects 0.000 claims description 4
- 150000002576 ketones Chemical class 0.000 claims description 4
- PORPENFLTBBHSG-MGBGTMOVSA-N 1,2-dihexadecanoyl-sn-glycerol-3-phosphate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(O)=O)OC(=O)CCCCCCCCCCCCCCC PORPENFLTBBHSG-MGBGTMOVSA-N 0.000 claims description 3
- 150000008282 halocarbons Chemical class 0.000 claims description 3
- 150000004292 cyclic ethers Chemical class 0.000 claims description 2
- 229930195733 hydrocarbon Natural products 0.000 claims description 2
- 150000002430 hydrocarbons Chemical class 0.000 claims description 2
- 239000004215 Carbon black (E152) Substances 0.000 claims 1
- 150000002894 organic compounds Chemical class 0.000 description 21
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 16
- 238000006243 chemical reaction Methods 0.000 description 16
- 150000001875 compounds Chemical class 0.000 description 15
- 102000004190 Enzymes Human genes 0.000 description 14
- 108090000790 Enzymes Proteins 0.000 description 14
- 239000000370 acceptor Substances 0.000 description 12
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 8
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- ATBOMIWRCZXYSZ-XZBBILGWSA-N [1-[2,3-dihydroxypropoxy(hydroxy)phosphoryl]oxy-3-hexadecanoyloxypropan-2-yl] (9e,12e)-octadeca-9,12-dienoate Chemical compound CCCCCCCCCCCCCCCC(=O)OCC(COP(O)(=O)OCC(O)CO)OC(=O)CCCCCCC\C=C\C\C=C\CCCCC ATBOMIWRCZXYSZ-XZBBILGWSA-N 0.000 description 7
- AWUCVROLDVIAJX-UHFFFAOYSA-N alpha-glycerophosphate Natural products OCC(O)COP(O)(O)=O AWUCVROLDVIAJX-UHFFFAOYSA-N 0.000 description 7
- 235000011187 glycerol Nutrition 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- JQWAHKMIYCERGA-UHFFFAOYSA-N (2-nonanoyloxy-3-octadeca-9,12-dienoyloxypropoxy)-[2-(trimethylazaniumyl)ethyl]phosphinate Chemical compound CCCCCCCCC(=O)OC(COP([O-])(=O)CC[N+](C)(C)C)COC(=O)CCCCCCCC=CCC=CCCCCC JQWAHKMIYCERGA-UHFFFAOYSA-N 0.000 description 6
- HCFAJYNVAYBARA-UHFFFAOYSA-N 4-heptanone Chemical compound CCCC(=O)CCC HCFAJYNVAYBARA-UHFFFAOYSA-N 0.000 description 6
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 6
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 6
- 230000004913 activation Effects 0.000 description 6
- 239000007864 aqueous solution Substances 0.000 description 6
- BTANRVKWQNVYAZ-UHFFFAOYSA-N butan-2-ol Chemical compound CCC(C)O BTANRVKWQNVYAZ-UHFFFAOYSA-N 0.000 description 6
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 6
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 5
- 150000002148 esters Chemical class 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 239000002994 raw material Substances 0.000 description 5
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 description 4
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 4
- XUPYJHCZDLZNFP-UHFFFAOYSA-N butyl butanoate Chemical compound CCCCOC(=O)CCC XUPYJHCZDLZNFP-UHFFFAOYSA-N 0.000 description 4
- 230000009849 deactivation Effects 0.000 description 4
- 239000003248 enzyme activator Substances 0.000 description 4
- PPXUHEORWJQRHJ-UHFFFAOYSA-N ethyl isovalerate Chemical compound CCOC(=O)CC(C)C PPXUHEORWJQRHJ-UHFFFAOYSA-N 0.000 description 4
- TZIHFWKZFHZASV-UHFFFAOYSA-N methyl formate Chemical compound COC=O TZIHFWKZFHZASV-UHFFFAOYSA-N 0.000 description 4
- FDPIMTJIUBPUKL-UHFFFAOYSA-N pentan-3-one Chemical compound CCC(=O)CC FDPIMTJIUBPUKL-UHFFFAOYSA-N 0.000 description 4
- 229940083466 soybean lecithin Drugs 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- DKPFZGUDAPQIHT-UHFFFAOYSA-N Butyl acetate Natural products CCCCOC(C)=O DKPFZGUDAPQIHT-UHFFFAOYSA-N 0.000 description 3
- NUPSHWCALHZGOV-UHFFFAOYSA-N Decyl acetate Chemical compound CCCCCCCCCCOC(C)=O NUPSHWCALHZGOV-UHFFFAOYSA-N 0.000 description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- 108010093096 Immobilized Enzymes Proteins 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 3
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
- 239000012190 activator Substances 0.000 description 3
- 230000001476 alcoholic effect Effects 0.000 description 3
- 150000001298 alcohols Chemical class 0.000 description 3
- PGMYKACGEOXYJE-UHFFFAOYSA-N anhydrous amyl acetate Natural products CCCCCOC(C)=O PGMYKACGEOXYJE-UHFFFAOYSA-N 0.000 description 3
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 3
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 229910001424 calcium ion Inorganic materials 0.000 description 3
- 229960001701 chloroform Drugs 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- FKRCODPIKNYEAC-UHFFFAOYSA-N ethyl propionate Chemical compound CCOC(=O)CC FKRCODPIKNYEAC-UHFFFAOYSA-N 0.000 description 3
- 150000002500 ions Chemical class 0.000 description 3
- 239000008363 phosphate buffer Substances 0.000 description 3
- WTJKGGKOPKCXLL-RRHRGVEJSA-N phosphatidylcholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCC=CCCCCCCCC WTJKGGKOPKCXLL-RRHRGVEJSA-N 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- VZGDMQKNWNREIO-UHFFFAOYSA-N tetrachloromethane Chemical compound ClC(Cl)(Cl)Cl VZGDMQKNWNREIO-UHFFFAOYSA-N 0.000 description 3
- XUCIJNAGGSZNQT-JHSLDZJXSA-N (R)-amygdalin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O[C@@H](C#N)C=2C=CC=CC=2)O1 XUCIJNAGGSZNQT-JHSLDZJXSA-N 0.000 description 2
- SCYULBFZEHDVBN-UHFFFAOYSA-N 1,1-Dichloroethane Chemical compound CC(Cl)Cl SCYULBFZEHDVBN-UHFFFAOYSA-N 0.000 description 2
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 description 2
- KBPLFHHGFOOTCA-UHFFFAOYSA-N 1-Octanol Chemical compound CCCCCCCCO KBPLFHHGFOOTCA-UHFFFAOYSA-N 0.000 description 2
- HNAGHMKIPMKKBB-UHFFFAOYSA-N 1-benzylpyrrolidine-3-carboxamide Chemical compound C1C(C(=O)N)CCN1CC1=CC=CC=C1 HNAGHMKIPMKKBB-UHFFFAOYSA-N 0.000 description 2
- BBMCTIGTTCKYKF-UHFFFAOYSA-N 1-heptanol Chemical compound CCCCCCCO BBMCTIGTTCKYKF-UHFFFAOYSA-N 0.000 description 2
- ZWEHNKRNPOVVGH-UHFFFAOYSA-N 2-Butanone Chemical compound CCC(C)=O ZWEHNKRNPOVVGH-UHFFFAOYSA-N 0.000 description 2
- PTTPXKJBFFKCEK-UHFFFAOYSA-N 2-Methyl-4-heptanone Chemical compound CC(C)CC(=O)CC(C)C PTTPXKJBFFKCEK-UHFFFAOYSA-N 0.000 description 2
- RXGUIWHIADMCFC-UHFFFAOYSA-N 2-Methylpropyl 2-methylpropionate Chemical compound CC(C)COC(=O)C(C)C RXGUIWHIADMCFC-UHFFFAOYSA-N 0.000 description 2
- 241000186361 Actinobacteria <class> Species 0.000 description 2
- 240000007124 Brassica oleracea Species 0.000 description 2
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 2
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 2
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 2
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 2
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- XINCECQTMHSORG-UHFFFAOYSA-N Isoamyl isovalerate Chemical compound CC(C)CCOC(=O)CC(C)C XINCECQTMHSORG-UHFFFAOYSA-N 0.000 description 2
- NTIZESTWPVYFNL-UHFFFAOYSA-N Methyl isobutyl ketone Chemical compound CC(C)CC(C)=O NTIZESTWPVYFNL-UHFFFAOYSA-N 0.000 description 2
- UIHCLUNTQKBZGK-UHFFFAOYSA-N Methyl isobutyl ketone Natural products CCC(C)C(C)=O UIHCLUNTQKBZGK-UHFFFAOYSA-N 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- IMNFDUFMRHMDMM-UHFFFAOYSA-N N-Heptane Chemical compound CCCCCCC IMNFDUFMRHMDMM-UHFFFAOYSA-N 0.000 description 2
- OFBQJSOFQDEBGM-UHFFFAOYSA-N Pentane Chemical compound CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical compound CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 description 2
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 2
- YRKCREAYFQTBPV-UHFFFAOYSA-N acetylacetone Chemical compound CC(=O)CC(C)=O YRKCREAYFQTBPV-UHFFFAOYSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- 150000007933 aliphatic carboxylic acids Chemical class 0.000 description 2
- 229940089837 amygdalin Drugs 0.000 description 2
- YZLOSXFCSIDECK-UHFFFAOYSA-N amygdalin Natural products OCC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC(C#N)c3ccccc3 YZLOSXFCSIDECK-UHFFFAOYSA-N 0.000 description 2
- MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 description 2
- OBNCKNCVKJNDBV-UHFFFAOYSA-N butanoic acid ethyl ester Natural products CCCC(=O)OCC OBNCKNCVKJNDBV-UHFFFAOYSA-N 0.000 description 2
- 229950005499 carbon tetrachloride Drugs 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- MWKFXSUHUHTGQN-UHFFFAOYSA-N decan-1-ol Chemical compound CCCCCCCCCCO MWKFXSUHUHTGQN-UHFFFAOYSA-N 0.000 description 2
- WDAXFOBOLVPGLV-UHFFFAOYSA-N ethyl isobutyrate Chemical compound CCOC(=O)C(C)C WDAXFOBOLVPGLV-UHFFFAOYSA-N 0.000 description 2
- YGHHWSRCTPQFFC-UHFFFAOYSA-N eucalyptosin A Natural products OC1C(O)C(O)C(CO)OC1OC1C(OC(C#N)C=2C=CC=CC=2)OC(CO)C(O)C1O YGHHWSRCTPQFFC-UHFFFAOYSA-N 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- WBJINCZRORDGAQ-UHFFFAOYSA-N formic acid ethyl ester Natural products CCOC=O WBJINCZRORDGAQ-UHFFFAOYSA-N 0.000 description 2
- ZSIAUFGUXNUGDI-UHFFFAOYSA-N hexan-1-ol Chemical compound CCCCCCO ZSIAUFGUXNUGDI-UHFFFAOYSA-N 0.000 description 2
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 2
- MLFHJEHSLIIPHL-UHFFFAOYSA-N isoamyl acetate Chemical compound CC(C)CCOC(C)=O MLFHJEHSLIIPHL-UHFFFAOYSA-N 0.000 description 2
- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 125000000962 organic group Chemical group 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- XNLICIUVMPYHGG-UHFFFAOYSA-N pentan-2-one Chemical compound CCCC(C)=O XNLICIUVMPYHGG-UHFFFAOYSA-N 0.000 description 2
- YKYONYBAUNKHLG-UHFFFAOYSA-N propyl acetate Chemical compound CCCOC(C)=O YKYONYBAUNKHLG-UHFFFAOYSA-N 0.000 description 2
- 239000000600 sorbitol Substances 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- DURPTKYDGMDSBL-UHFFFAOYSA-N 1-butoxybutane Chemical compound CCCCOCCCC DURPTKYDGMDSBL-UHFFFAOYSA-N 0.000 description 1
- UFBJCMHMOXMLKC-UHFFFAOYSA-N 2,4-dinitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O UFBJCMHMOXMLKC-UHFFFAOYSA-N 0.000 description 1
- UHOPWFKONJYLCF-UHFFFAOYSA-N 2-(2-sulfanylethyl)isoindole-1,3-dione Chemical compound C1=CC=C2C(=O)N(CCS)C(=O)C2=C1 UHOPWFKONJYLCF-UHFFFAOYSA-N 0.000 description 1
- MSWZFWKMSRAUBD-GASJEMHNSA-N 2-amino-2-deoxy-D-galactopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@H](O)[C@@H]1O MSWZFWKMSRAUBD-GASJEMHNSA-N 0.000 description 1
- MSWZFWKMSRAUBD-IVMDWMLBSA-N 2-amino-2-deoxy-D-glucopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O MSWZFWKMSRAUBD-IVMDWMLBSA-N 0.000 description 1
- IQUPABOKLQSFBK-UHFFFAOYSA-N 2-nitrophenol Chemical compound OC1=CC=CC=C1[N+]([O-])=O IQUPABOKLQSFBK-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- XDTMQSROBMDMFD-UHFFFAOYSA-N Cyclohexane Chemical compound C1CCCCC1 XDTMQSROBMDMFD-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 244000000626 Daucus carota Species 0.000 description 1
- 235000002767 Daucus carota Nutrition 0.000 description 1
- IEPRKVQEAMIZSS-UHFFFAOYSA-N Di-Et ester-Fumaric acid Natural products CCOC(=O)C=CC(=O)OCC IEPRKVQEAMIZSS-UHFFFAOYSA-N 0.000 description 1
- IEPRKVQEAMIZSS-WAYWQWQTSA-N Diethyl maleate Chemical compound CCOC(=O)\C=C/C(=O)OCC IEPRKVQEAMIZSS-WAYWQWQTSA-N 0.000 description 1
- DKMROQRQHGEIOW-UHFFFAOYSA-N Diethyl succinate Chemical compound CCOC(=O)CCC(=O)OCC DKMROQRQHGEIOW-UHFFFAOYSA-N 0.000 description 1
- ZAFNJMIOTHYJRJ-UHFFFAOYSA-N Diisopropyl ether Chemical compound CC(C)OC(C)C ZAFNJMIOTHYJRJ-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- ICMAFTSLXCXHRK-UHFFFAOYSA-N Ethyl pentanoate Chemical compound CCCCC(=O)OCC ICMAFTSLXCXHRK-UHFFFAOYSA-N 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- JZNWSCPGTDBMEW-UHFFFAOYSA-N Glycerophosphorylethanolamin Natural products NCCOP(O)(=O)OCC(O)CO JZNWSCPGTDBMEW-UHFFFAOYSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- NHTMVDHEPJAVLT-UHFFFAOYSA-N Isooctane Chemical compound CC(C)CC(C)(C)C NHTMVDHEPJAVLT-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- OVRNDRQMDRJTHS-CBQIKETKSA-N N-Acetyl-D-Galactosamine Chemical compound CC(=O)N[C@H]1[C@@H](O)O[C@H](CO)[C@H](O)[C@@H]1O OVRNDRQMDRJTHS-CBQIKETKSA-N 0.000 description 1
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 1
- 102100032967 Phospholipase D1 Human genes 0.000 description 1
- NGFMICBWJRZIBI-JZRPKSSGSA-N Salicin Natural products O([C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O1)c1c(CO)cccc1 NGFMICBWJRZIBI-JZRPKSSGSA-N 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 241000187747 Streptomyces Species 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- DOOTYTYQINUNNV-UHFFFAOYSA-N Triethyl citrate Chemical compound CCOC(=O)CC(O)(C(=O)OCC)CC(=O)OCC DOOTYTYQINUNNV-UHFFFAOYSA-N 0.000 description 1
- 125000002252 acyl group Chemical group 0.000 description 1
- 229960005305 adenosine Drugs 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- NGFMICBWJRZIBI-UHFFFAOYSA-N alpha-salicin Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=CC=C1CO NGFMICBWJRZIBI-UHFFFAOYSA-N 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 229940072049 amyl acetate Drugs 0.000 description 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical class OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- IEPRKVQEAMIZSS-AATRIKPKSA-N diethyl fumarate Chemical compound CCOC(=O)\C=C\C(=O)OCC IEPRKVQEAMIZSS-AATRIKPKSA-N 0.000 description 1
- YSAVZVORKRDODB-WDSKDSINSA-N diethyl tartrate Chemical compound CCOC(=O)[C@@H](O)[C@H](O)C(=O)OCC YSAVZVORKRDODB-WDSKDSINSA-N 0.000 description 1
- JVSWJIKNEAIKJW-UHFFFAOYSA-N dimethyl-hexane Natural products CCCCCC(C)C JVSWJIKNEAIKJW-UHFFFAOYSA-N 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- YYZUSRORWSJGET-UHFFFAOYSA-N ethyl octanoate Chemical compound CCCCCCCC(=O)OCC YYZUSRORWSJGET-UHFFFAOYSA-N 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 239000001530 fumaric acid Substances 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 229940052308 general anesthetics halogenated hydrocarbons Drugs 0.000 description 1
- 229960002442 glucosamine Drugs 0.000 description 1
- 150000002327 glycerophospholipids Chemical group 0.000 description 1
- 150000002337 glycosamines Chemical class 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- MNWFXJYAOYHMED-UHFFFAOYSA-M heptanoate Chemical compound CCCCCCC([O-])=O MNWFXJYAOYHMED-UHFFFAOYSA-M 0.000 description 1
- FUZZWVXGSFPDMH-UHFFFAOYSA-N hexanoic acid Chemical compound CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- GJRQTCIYDGXPES-UHFFFAOYSA-N iso-butyl acetate Natural products CC(C)COC(C)=O GJRQTCIYDGXPES-UHFFFAOYSA-N 0.000 description 1
- 229940117955 isoamyl acetate Drugs 0.000 description 1
- FGKJLKRYENPLQH-UHFFFAOYSA-M isocaproate Chemical compound CC(C)CCC([O-])=O FGKJLKRYENPLQH-UHFFFAOYSA-M 0.000 description 1
- JMMWKPVZQRWMSS-UHFFFAOYSA-N isopropanol acetate Natural products CC(C)OC(C)=O JMMWKPVZQRWMSS-UHFFFAOYSA-N 0.000 description 1
- 229940011051 isopropyl acetate Drugs 0.000 description 1
- GWYFCOCPABKNJV-UHFFFAOYSA-N isovaleric acid Chemical compound CC(C)CC(O)=O GWYFCOCPABKNJV-UHFFFAOYSA-N 0.000 description 1
- OQAGVSWESNCJJT-UHFFFAOYSA-N isovaleric acid methyl ester Natural products COC(=O)CC(C)C OQAGVSWESNCJJT-UHFFFAOYSA-N 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 239000000696 magnetic material Substances 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 229940042880 natural phospholipid Drugs 0.000 description 1
- 238000010979 pH adjustment Methods 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 150000008104 phosphatidylethanolamines Chemical class 0.000 description 1
- 108010002266 phospholipase D1 Proteins 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- XCRZAIPPBHIKOR-UHFFFAOYSA-N prop-2-enyl 4-amino-3-fluorobenzoate Chemical compound NC1=CC=C(C(=O)OCC=C)C=C1F XCRZAIPPBHIKOR-UHFFFAOYSA-N 0.000 description 1
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 1
- MCSINKKTEDDPNK-UHFFFAOYSA-N propyl propionate Chemical compound CCCOC(=O)CC MCSINKKTEDDPNK-UHFFFAOYSA-N 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- NGFMICBWJRZIBI-UJPOAAIJSA-N salicin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=CC=C1CO NGFMICBWJRZIBI-UJPOAAIJSA-N 0.000 description 1
- 229940120668 salicin Drugs 0.000 description 1
- DCKVNWZUADLDEH-UHFFFAOYSA-N sec-butyl acetate Chemical compound CCC(C)OC(C)=O DCKVNWZUADLDEH-UHFFFAOYSA-N 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- RPACBEVZENYWOL-XFULWGLBSA-M sodium;(2r)-2-[6-(4-chlorophenoxy)hexyl]oxirane-2-carboxylate Chemical compound [Na+].C=1C=C(Cl)C=CC=1OCCCCCC[C@]1(C(=O)[O-])CO1 RPACBEVZENYWOL-XFULWGLBSA-M 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- 239000001069 triethyl citrate Substances 0.000 description 1
- VMYFZRTXGLUXMZ-UHFFFAOYSA-N triethyl citrate Natural products CCOC(=O)C(O)(C(=O)OCC)C(=O)OCC VMYFZRTXGLUXMZ-UHFFFAOYSA-N 0.000 description 1
- 235000013769 triethyl citrate Nutrition 0.000 description 1
- 241001446247 uncultured actinomycete Species 0.000 description 1
- 239000008096 xylene Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P9/00—Preparation of organic compounds containing a metal or atom other than H, N, C, O, S or halogen
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Description
ç£æ¥äžã®å©çšåé
倧è±ã¬ã·ãã³ãåµé»ã¬ã·ãã³çã®ãªã³è質ã¯ã
ãã®çé¢æŽ»æ§ãççäœçšã掻ãããŠãé£åãå粧
åãå¡æã最æ»æ²¹ãç£æ§ææãè©Šæãå»è¬åã蟲
è¬çã®è£œé ã«åºãå©çšãããŠãããããªã³è質ãš
æ°Žé žåºå«æååç©ã«ãã¹ãã¢ããžã«åºè»¢ç§»äœçšã
æããé µçŽ ãäœçšãããŠåŸãããæ°ããªãã¹ãã¢
ããžã³é žèªå°äœããåæã®ãªã³è質以äžã«æå©ãª
æ§è³ªã瀺ãæçšç©è³ªã§ããããšãå€ããæ¬çºæ
ã¯ããªã³è質ãåæã«ããŠäžèšãã¹ãã¢ããžã«åº
転移åå¿ã«ãããã¹ãã¢ããžã³é žèªå°äœã補é ã
ãæ¹æ³ã®æ¹è¯ã«é¢ãããã®ã§ããã åŸæ¥ã®æè¡ ãã¹ãã¢ããžã³é žã®èªå°äœã§ãããªã³è質ã«æ°Ž
ã®ååšäžã§ãã¹ããªããŒãŒïŒ€ãäœçšããããšãå
æ°Žå解ãèµ·ãããŠãã¹ãã¢ããžã³é žãçãããã
ãã®éããã¹ãã¢ããžã«åºã®å容äœãšãªãåŸãã¢
ã«ã³ãŒã«æ§æ°Žé žåºå«æååç©ããã³ïŒäŸ¡ã®éå±ã€
ãªã³ãå ±åããããšãäžèšã®ãããªãã¹ãã¢ããž
ã«åºè»¢ç§»åå¿ãèµ·ãããŠæ°ããªãã¹ãã¢ããžã³é ž
èªå°äœãçããããšãç¥ãããŠããã
ãã®çé¢æŽ»æ§ãççäœçšã掻ãããŠãé£åãå粧
åãå¡æã最æ»æ²¹ãç£æ§ææãè©Šæãå»è¬åã蟲
è¬çã®è£œé ã«åºãå©çšãããŠãããããªã³è質ãš
æ°Žé žåºå«æååç©ã«ãã¹ãã¢ããžã«åºè»¢ç§»äœçšã
æããé µçŽ ãäœçšãããŠåŸãããæ°ããªãã¹ãã¢
ããžã³é žèªå°äœããåæã®ãªã³è質以äžã«æå©ãª
æ§è³ªã瀺ãæçšç©è³ªã§ããããšãå€ããæ¬çºæ
ã¯ããªã³è質ãåæã«ããŠäžèšãã¹ãã¢ããžã«åº
転移åå¿ã«ãããã¹ãã¢ããžã³é žèªå°äœã補é ã
ãæ¹æ³ã®æ¹è¯ã«é¢ãããã®ã§ããã åŸæ¥ã®æè¡ ãã¹ãã¢ããžã³é žã®èªå°äœã§ãããªã³è質ã«æ°Ž
ã®ååšäžã§ãã¹ããªããŒãŒïŒ€ãäœçšããããšãå
æ°Žå解ãèµ·ãããŠãã¹ãã¢ããžã³é žãçãããã
ãã®éããã¹ãã¢ããžã«åºã®å容äœãšãªãåŸãã¢
ã«ã³ãŒã«æ§æ°Žé žåºå«æååç©ããã³ïŒäŸ¡ã®éå±ã€
ãªã³ãå ±åããããšãäžèšã®ãããªãã¹ãã¢ããž
ã«åºè»¢ç§»åå¿ãèµ·ãããŠæ°ããªãã¹ãã¢ããžã³é ž
èªå°äœãçããããšãç¥ãããŠããã
ãåã
ïŒãªã³è質ïŒ
ïŒåŒäžãR1ãR2ã¯ã¢ã·ã«åºãã¢ã«ãã«åºçãX1
ã¯ç³ãŸãã¯ã¢ã«ã³ãŒã«æ§æ°Žé žåºå«æå¡©åºã®æ°Žé žåº
éšåã®æ°ŽçŽ ååïŒåãé€ããåŸã«æ®ãææ©åºã
X2ã¯çŽéèèªæå€äŸ¡ã¢ã«ã³ãŒã«ãç³ãªã©ã®æ°Žé ž
åºå«æååç©ãããã®æ°Žé žåºéšåã®æ°ŽçŽ ååïŒå
ãé€ããåŸã«æ®ãææ©åºãè¡šãããïŒ ïŒäŸ¡éå±ã€ãªã³ã¯ããã¹ããªããŒãŒïŒ€ã«ãã¹ã
ã¢ããžã«åºè»¢ç§»åå¿æŽ»æ§ãçºçŸãããã®ã«äžå¯æ¬
ã®ãã®ãšèããããŠããïŒäœãããã¹ãã¢ããžã«
ã°ãªã»ããŒã«ãšããåäžãªã³è質ååéã§ã®ãã¹
ãã¢ããžã«åºè»¢ç§»åå¿ã«ãããŠã¯äŸå€çã«ïŒäŸ¡é
å±ã€ãªã³ã®æ·»å ãå¿ èŠãšããªãã€ããšããå ±åâ
Can.J.Biochem.ã51ã747ã1973âãããïŒãã
ããã€ãŠãåŸæ¥ãäžè¬çãªç°çš®ååéãã¹ãã¢ã
ãžã«åºè»¢ç§»åå¿ã«ãããã¹ãã¢ããžã³é žèªå°äœã
補é ããå Žåã¯å¿ ãïŒäŸ¡éå±ã€ãªã³ïŒæ®éã¯Ca
ã€ãªã³ïŒã«ãã€ãŠãã¹ããªããŒãŒïŒ€ã®æŽ»æ§åãè¡
ãªã€ãŠããïŒç¹éæ58â63388å·ãå58â67183
å·ãå59â187792å·ãå62â36195å·çïŒã ãã¹ããªããŒãŒïŒ€ã®æŽ»æ§åã«Caã€ãªã³ãçšã
ãå Žåãåå¿ç³»ã«ã¯ã€ãªã³ãšåå¿æ§ããç©è³ªãå
ããããšãã§ããªããããŸããæ°Žé žåºå«æååç©
ã§ãã€ãŠãCaã€ãªã³ãšååããŠåå¿æ§ã倱ãã
ããªãã®ã¯ãã¹ãã¢ããžã«åºå容äœãšããŠäœ¿ãã
ãšãã§ããªããããšãã°ã転移åå¿ã®è³é©PHãïŒ
ãïŒä»è¿ã«ããé µçŽ ãçšããå Žåãæã䜿ãæã
äžã€å®äŸ¡ãªãªã³é žç·©è¡æ¶²ã¯Caã€ãªã³ãšåå¿ããŠ
æ²æ®¿ãçãããã䜿ãããšãã§ãããããé«äŸ¡ãª
ç·©è¡æ¶²ã䜿çšããããåŸãªãããŸããç®çç©ã®äž
éšã«Caå¡©åã®ãã®ãã§ãããããNaå¡©åãªã©ä»
ã®å¡©åã®ãã®ã補é ããå Žåã¯ãã¬ãŒãå€ãçšã
ããããŠç®çãšããå¡©ã«å€æãããªã©ã®æäœãå¿
èŠã§ããã çºæã解決ããããšããåé¡ç¹ æ¬çºæã®ç®çã¯ããªã³è質ãšãã¹ããªããŒãŒïŒ€
ãçšããåŸæ¥ã®ãã¹ãã¢ããžã³é žèªå°äœã®è£œæ³ã
äžè¿°ã®ããã«ïŒäŸ¡éå±ã€ãªã³ã®äœ¿çšã«ãšããªã
çš®ã ã®å¶çŽãããã®ã§ãã€ãããšã«éã¿ãïŒäŸ¡é
å±ã€ãªã³ãçšããªããã¹ããªããŒãŒïŒ€ã®æŽ»æ§åæ³
ã«ãã補æ³ãæäŸããŠããã¹ãã¢ããžã³é žèªå°äœ
ã®è£œé ãåŸæ¥ããã容æã«ããããšã«ããã åé¡ç¹ã解決ããããã®æ段 äžèšç®çãéæããããšã«æåããæ¬çºæã®è£œ
æ³ã¯ããã¹ããªããŒãŒïŒ€ã®æŽ»æ§åã«ïŒäŸ¡éå±ã€ãª
ã³ãçšããããããã«ççŽ æ°ïŒã12ã®ãšã¹ãã«
é¡ãççŽ æ°ïŒãïŒã®ã±ãã³é¡ãççŽ æ°ïŒãïŒã®ç
åæ°ŽçŽ ãççŽ æ°ïŒãïŒã®ããã²ã³åçåæ°ŽçŽ ãç
çŽ æ°ïŒãïŒã®èèªæãšãŒãã«é¡ãããã¯ç°ç¶ãšãŒ
ãã«é¡ããŸãã¯ããšãã«ã¡ã«ã«ãã¿ã³ãçšããŠæŽ»
æ§åãè¡ãããšãç¹åŸŽãšãããã®ã§ããã ææ©æº¶åªããšãã°ãžãšãã«ãšãŒãã«ã¯ãåŸæ¥ã®
補æ³ã«ãããŠããã°ãã°åå¿ç³»æ§ææåãšããŠçš
ããããŠãããããããªãããåŸæ¥ã®è£œæ³ã«ãã
ãŠã¯ãææ©æº¶åªã¯æ°Žã«é£æº¶æ§ã®ãªã³è質ã溶ãã
ãŠé µçŽ ããã³ãã¹ãã¢ããžã«åºå容äœã®æ°Žæº¶æ¶²ãš
æ¥è§Šãããããã®åãªã溶åªãšããŠäœ¿ãããŠã
ããé µçŽ ã®æŽ»æ§åã«ã¯å¿ ãCaã€ãªã³çã®ïŒäŸ¡é
å±ã€ãªã³ãå¥ã«äœ¿ãããŠããã ïŒäŸ¡éå±ã€ãªã³ãçšããææ©ååç©ã«ããé µçŽ
掻æ§åãè¡ããã®çºæã¯ãæ¬çºæè ãã«ãã次ã®
ãããªæ°èŠãªç¥èŠã«åºã¥ããã®ã§ããã (a) ããçš®ã®ææ©ååç©ïŒããšãã°é ¢é žãšãã«ã
ïŒâããã¿ãã³çïŒã¯ãåå¿ç³»ã«ããæ¯ç以äž
ã«å ããããå ŽåãïŒäŸ¡éå±ã€ãªã³ãååšããª
ããŠããã¹ããªããŒãŒïŒ€ã掻æ§åããã (b) ããçš®ã®ææ©ååç©ïŒããšãã°äœçŽèèªæäž
çŽã¢ã«ã³ãŒã«ããžã¡ãã«ãã«ã ã¢ããããžã¡ã
ã«ã¹ã«ããã·ãçïŒã¯ããã¹ããªããŒãŒïŒ€ãã»
ãšãã©æŽ»æ§åãããããã倱掻ãããã (c) ãã¹ããªããŒãŒïŒ€ã掻æ§åããååç©ã®äžã«
ããããæ¿åºŠä»¥äžã§ã¯ãã¹ããªããŒãŒïŒ€ã倱掻
ããããã®ãå€ãïŒããšãã°åå¡©åççŽ ãïœâ
ãããµã³ããã³ãŒã³ãïŒâãã¿ããŒã«ããžãªã
ãµã³çïŒã (d) ãã¹ããªããŒãŒïŒ€ã«å¯ŸããŠäºå®äžäžæŽ»æ§ãªæ
æ©ååç©ãããªãã¡ãã¹ããªããŒãŒïŒ€ã掻æ§å
ããããŸã倱掻ããããªãååç©ãããã ãããã€ãŠãæ¬çºæã«ãããŠã¯ããã¹ããªããŒ
ãŒïŒ€ã«ãã¹ãã¢ããžã«åºè»¢ç§»åå¿æŽ»æ§ãçºçŸãã
ãäœçšãæããææ©ååç©ããäžèšæŽ»æ§åäœçšã®
ã»ããé µçŽ å€±æŽ»äœçšããã匷ãçŸããããããªæ¡
件ã§çšããŠããã¹ããªããŒãŒïŒ€ã掻æ§åããã é©é䜿çšããå Žåã«ãã¹ããªããŒãŒïŒ€ã掻æ§å
ãããšãšãã«ãªã³è質ã溶解ãããã¯åæ£ããã
èœåã®ç¹ã§ããããããããã€ãŠæ¬çºæã®è£œæ³ã«
ãããŠäœ¿çšããé µçŽ æŽ»æ§åçšææ©ååç©ãšããŠå¥œ
ãŸãããã®ã®å ·äœäŸã瀺ããšã次ã®ãããªãã®ã
ããã ãšã¹ãã«é¡ïŒççŽ æ°ïŒã10ã®èèªæã«ã«ãã³é ž
ããã³åãççŽ æ°ç¯å²ã®èèªæã¢ã«ã³ãŒã«ããåŸ
ãããççŽ æ°ïŒã12ã®ãšã¹ãã«é¡ãç¹ã«å¥œãŸã
ããããšãã°é ¢é žãšãã«ãé ¢é žããã«ãé ¢é žã¢ã
ã«ãé ¢é žãã·ã«ãããããªã³é žãšãã«ãããããª
ã³é žãããã«ãé ªé žãšãã«ãé ªé žããã«ãã€ãœé ª
é žã€ãœããã«ãã€ãœåèé žãšãã«ãªã©ã§ããããª
ãã§ãé ¢é žãšãã«ã¯ãïŒäŸ¡éå±å¡©ãçšããåŸæ¥ã®
補æ³ã§ã¯ãšããŠãäžå¯èœã§ãã€ããããªé«çïŒçŽ
90ïŒ ä»¥äžïŒã§ãã¹ãã¢ããžã«åºè»¢ç§»åå¿ãçèµ·ã
ãããã¹ãã¢ããžã³é žã®å¹ æãå°ãªãã ãã§ãª
ããåæ±ãã容æã§äŸ¡æ Œãäœå»ã§ãããããæ¬çº
æã®è£œæ³ã§çšãããã¹ããªããŒãŒïŒ€æŽ»æ§åå€ãšã
ãŠæã奜ãŸãããã®é žã¡ãã«ãã®é žãšãã«ã¯é µçŽ
倱掻äœçšããã匷ãã䜿ãé£ããççŽ æ°11以äžã®
é«çŽèèªæã«ã«ãã³é žãã¢ã«ã³ãŒã«ããåŸããã
ãšã¹ãã«ããããã¯ããããã·ã«ã«ãã³é žé¡ïŒã
ãšãã°ä¹³é žãããã«é žãã¯ãšã³é žçïŒã®ãšã¹ãã«
ã¯ããã¹ããªããŒãŒïŒ€æŽ»æ§åäœçšã匱ãã ã±ãã³é¡ïŒççŽ æ°ïŒãïŒã®èèªå±ã±ãã³ãããš
ãã°ïŒâãã³ã¿ãã³ãã¡ãã«ã€ãœããã«ã±ãã³ã
ïŒâããã¿ãã³ããžã€ãœããã«ã±ãã³ããç¹ã«å¥œ
ãŸãããããããã³ããã¿ãã³ãªã©ãççŽ æ°ãïŒ
æªæºã®ãã®ã¯ãé µçŽ å€±æŽ»äœçšã匷ãã ããã²ã³åçåæ°ŽçŽ ïŒãžã¯ãã«ã¡ã¿ã³ãããªã¯
ãã«ã¡ã¿ã³ããžã¯ãã«ãšã¿ã³çã奜ãŸããã åè¿°ã®ããã«ãããã奜ãŸããææ©ååç©ãšã
ãã©ã䜿çšéã«ãã€ãŠã¯é µçŽ ã倱掻ãããããšã
ããããŸã倱掻äœçšã匷ãçŸããæ¿åºŠããããã
ç°ãªãããã奜é©äœ¿çšéã¯ååç©ã«ãã€ãŠç°ãªã
ããå€ãã®å Žåã奜é©äœ¿çšéã¯æ°ŽïŒå®¹ééšã«å¯Ÿã
ãŠé µçŽ 掻æ§åçšææ©ååç©0.05ã50容ééšã®ç¯å²
ã«ãããåã ã®ææ©ååç©ã«ã€ããŠã®æé©äœ¿çšé
ã¯ãå®éšã«ãã容æã«ç¢ºèªããããšãã§ããã æ¬çºæã®è£œæ³ã«ãããŠãåæã®ãªã³è質ãšããŠ
ã¯ã°ãªã»ããªã³è質ã§ããã°ãããªããªã³è質ã
䜿çšããããšãã§ããããšãã°å€§è±ã¬ã·ãã³ãåµ
é»ã¬ã·ãã³ãªã©å€©ç¶ã®ãã®ã®ã»ãããã¹ãã¢ããž
ã«ã³ã³ããã¹ãã¢ããžã«ãšã¿ããŒã«ã¢ãã³ãªã©ã®
åæåãçšããããšãã§ããã ããŸäžã€ã®åå¿åæã§ããâãã¹ãã¢ããžã«åº
å容äœâãšãªãåŸãååç©ã¯ãæ°Žé žåºãæããå
åç©ãããªãã¡ã¡ã¿ããŒã«ããšã¿ããŒã«ãããã
ããŒã«ãïŒâãããããŒã«ããã¿ããŒã«ãïŒâã
ã¿ããŒã«ãã€ãœã¢ãã«ã¢ã«ã³ãŒã«ããããµããŒ
ã«ãããã¿ããŒã«ããªã¯ã¿ããŒã«ããã«ããŒã«ã
ã°ãªã»ãªã³ããšãã¬ã³ã°ãªã³ãŒã«çã®ã¢ã«ã³ãŒã«
é¡ïŒã°ãªã³ãŒã¹ããã·ããŒã¹ãã¢ã©ãããŒã¹ãã
ã«ã¯ããŒã¹ãβââã°ãªã³ãŒã¹ããã³ããŒã¹ã
ã¹ã¯ããŒã¹ãã©ã¯ããŒã¹ãã»ãããªãŒã¹ãã¡ãªã
ãªãŒã¹çã®ç³é¡ïŒã°ã«ã³ãµãã³ãã¬ã©ã¯ããµã
ã³ãã¢ã»ãã«ã¬ã©ã¯ããµãã³çã®ã¢ããç³ïŒãœã«
ãããŒã«ããã³ãããŒã«çã®ã¢ã«ã³ãŒã«ïŒã¢ãã°
ããªã³ããµãªã·ã³çã®é ç³äœïŒããšããŒã«ããã
ãããšããŒã«ããžãããããšããŒã«çã®ããšããŒ
ã«é¡ïŒãã®ä»ïŒããšãã°ã¢ããã·ã³ïŒã®äžããä»»
æã«éžã°ããã ãªããæ¬çºæã®ç®çã¯åå¿æ¹æ³ã®æ¹è¯ã«ããã
ç¹å®ã®ãã¹ãã¢ããžã³é žèªå°äœãæäŸããããšã«
ããã®ã§ã¯ãªãããããããªããªã³è質ãšãã¹ã
ã¢ããžã«åºå容äœãšãçµåããŠåå¿ããããã¯å š
ãèªç±ã§ããïŒæ¬çºæã®æ¹æ³ã«ãããŠã®ã¿ãã¹ã
ã¢ããžã«åºè»¢ç§»åå¿ãçãåŸãªããªã³è質ã¯èŠã
ã ãããŠããªãïŒã ãã¹ããªããŒãŒïŒ€ãšããŠã¯ããã€ããããã³ãž
ã³ãããŒãããªã©ã®æ€ç©äœããæœåºããããã®
ãšã埮çç©ç±æ¥ã®ãã®ãšããããæ¬çºæã«ãããŠ
ã¯ãã®ããããçšããŠããããã埮çç©ãç¹ã«æŸ
ç·èç±æ¥ã®ãã¹ããªããŒãŒïŒ€ã¯ãç±å®å®æ§ã«ãã
ãããšãšãã«æŽ»æ§ã匷ãã®ã§å¥œãŸãããæ¬çºæã®
補æ³ã«ãããŠããã¹ããªããŒãŒïŒ€ã¯åå¿ç³»ã«æº¶è§£
ãããŠçšããã»ããåºå®åé µçŽ ã®åœ¢ã§çšããŠãã
ãã ãªã³è質ããã¹ãã¢ããžã«åºå容äœãšãªãåå
ç©ããã¹ããªããŒãŒïŒ€ããã³ãã®æŽ»æ§åå€ãšãªã
ææ©ååç©ãæ··åããŠãã¹ãã¢ããžã«åºè»¢ç§»åå¿
ãçèµ·ãããå Žåãæ··åé ã¯ç¹ã«éå®ãããªã
ããäœè¡šçãªæ¹æ³ã¯æ¬¡ã®ãšããã§ããã  ãªã³è質ãæ°Žäžã«åæ£ãæžæ¿ãããããã«ã
ã¹ãã¢ããžã«åºå容äœãšãªãååç©ããã¹ããª
ããŒãŒïŒ€æº¶æ¶²ããã³é µçŽ 掻æ§åå€ãšããææ©å
åç©ãå ããŠæªæãæ··åããã  é µçŽ æŽ»æ§åå€ãšããææ©ååç©ã«ãªã³è質ã
溶解ãããã¹ãã¢ããžã«åºå容äœãšãªãååç©
ããã³ãã¹ããªããŒãŒïŒ€ã®æ°Žæº¶æ¶²ãšæ··åããã  é µçŽ æŽ»æ§åå€ãšããææ©ååç©ã«ãªã³è質ã
ãã³ãã¹ãã¢ããžã«åºå容äœãšãªãååç©ã溶
解ãããã¹ããªããŒãŒïŒ€ã®æ°Žæº¶æ¶²ãšæ··åããã åå¿ç³»ã«ã¯ãå¿ èŠã«å¿ããŠä»ã«ä»»æã®è£å©çæ
åãããšãã°PH調æŽã®ããç·©è¡å€ããªã³è質ãŸã
ã¯ãã®æº¶æ¶²ãåæ£ãããããã®çé¢æŽ»æ§å€çãã
é µçŽ äœçšã®åŠšããšãªããªãç¯å²ã§å ããããšãã§
ãããäœãïŒäŸ¡éå±ã€ãªã³ã¯ãææ©ååç©ã«ãã
ãã¹ããªããŒãŒïŒ€æŽ»æ§åã®åŠšããšãªãããã§ã¯ãª
ãããæ¬çºæã§ã¯äœ¿çšããªãã ãã¹ããªããŒãŒïŒ€ãåºå®åé µçŽ ã®åœ¢ã§çšããå Ž
åã¯ãåå¿ç³»ãæªæäžèŠã®å®å®ãªåäžçžã«ããŠã
ãããšãæãŸããããã®å Žåã¯ãåå¿ç³»æ§ææå
ãç¹å®ã®æ¯çã§é åããããšãã«åœ¢æãããéæ
ãªåäžçžãåºå®åé µçŽ ãšæ¥è§Šããããšãããäžèš
éæãªåäžçžã¯ãé µçŽ æŽ»æ§åçšã®ææ©ååç©ã«ãª
ã³è質ãæ¯èŒçé«æ¿åºŠã«æº¶è§£ããããã«ãã¹ãã¢
ããžã«åºå容äœãšãªãååç©ã®æ°Žæº¶æ¶²ãäžå®ã®é
床å ã§å ããŠæªæãããšããªã³è質ã®çé¢æŽ»æ§ã
é¢äžããŠåœ¢æãããããã®åäžçžã¯ãé µçŽ æŽ»æ§å
å€ãšããŠã®ææ©ååç©ä»¥å€ã®èŠªæ°Žæ§æº¶åªãå°éå
ãããšåœ¢æããæããªãããšããããããšãã°ã
åµé»ã¬ã·ãã³20ééïŒ ãã°ãªã»ãªã³ïŒééïŒ ãé ¢
é žãšãã«60ééïŒ ãïŒâãããããŒã«ïŒééïŒ ã
æ°Ž10ééïŒ ã®çµæã®æ··åç©ã¯ã容æã«ãéæãªå
äžçžã圢æããã çšããé µçŽ ã®è³é©æž©åºŠä»è¿ã§å åæéåå¿ãã
ãã®ã¡ãåå¿æ¶²ããã¢ã»ãã³ããšã¿ããŒã«ãçšã
ã溶åªåå¥æ³ãã·ãªã«ã²ã«ãã¢ã«ãããããã¯é
çžæ äœçãçšããã«ã©ã ã¯ãããã°ã©ãã€ãŒãè
çžã¯ãããã°ã©ãã€ãŒçã®æ¹æ³ã«ãããç®çãšã
ããã¹ãã¢ããžã³é žèªå°äœãååããããšãã§ã
ãã å®æœäŸ 以äžãå®æœäŸã瀺ããŠæ¬çºæã説æããã å®æœäŸ ïŒ åµé»ã¬ã·ãã³ãã調補ããããã¹ãã¢ããžã«ã³
ãªã³ïŒmgïŒã·ã°ã瀟ïŒã«ããã¹ãã¢ããžã«åºå容
äœãšããŠã°ãªã»ãªã³100ÎŒãPH6.0ã®ãªã³é žç·©è¡
液400ÎŒãæŸç·èç±æ¥ã®ãã¹ããªããŒãŒD1ãŠã
ãããå ããæ··åããã äžèšã®ããã«ããŠåŸãããæ··åç©ã«ãïŒã
100ÎŒã®é ¢é žãšãã«ãå ãã50âã«ä¿ã€ãŠæ¯ãš
ãããªããããã¹ãã¢ããžã«åºè»¢ç§»åå¿ã«ããã
ã¹ãã¢ããžã«ã°ãªã»ããŒã«ã®çæéãçµæçã«å®
éããããã®çµæã¯è¡šïŒã®ãšããã§ãã€ãŠãïŒäŸ¡
éå±ã€ãªã³ãå ããªããŠããé ¢é žãšãã«æ·»å é
30Ό以äžã®å Žåã«ãã¹ãã¢ããžã«ã°ãªã»ããŒã«
ãçæããã è¡šïŒ ãã¹ãã¢ããžã«ã°ãªã»ããŒã«çæéåºŠé ¢é žãšãã«æ·»å çæé床 ïŒÎŒïŒ ïŒÎŒgïŒunitã»minïŒ ïŒ ïŒ 10 ïŒ 30 35 50 124 75 143 100 101 å®æœäŸ ïŒ ãã¹ãã¢ããžã«ã³ãªã³å«æéãçŽ90ïŒ ã§ãã倧
è±ã¬ã·ãã³2.5ïœãæ¿åºŠ30ïŒ ïŒïœïŒïœïŒã®ãã¹ã
ã¢ããžã«åºå容äœæ°Žæº¶æ¶²100mlãæŸç·èç±æ¥ã®ã
ã¹ããªããŒãŒïŒ€æº¶æ¶²ïŒmlãé ¢é žãšãã«375mlãæ··
åããæªæããªãã50âã§60åéåå¿ãããã å容äœãçš®ã å€æŽããŠäžèšè£œé æ³ãå®æœããçµ
æãè¡šïŒã«ç€ºãããªããè¡šäžã®ãïŒ ãã¯ãåå¿æ¶²
äžã®æªåå¿ãªã³è質ãå«ãå šãã¹ãã¢ããžã³é žèª
å°äœåºæºã®ééïŒ ã§ããã è¡šïŒåå®¹äœ è»¢ç§»åå¿çæç©ïŒïŒ ïŒ ãã·ããŒã¹ 72.2 ã¬ã©ã¯ããŒã¹ 1.3 ã¢ãã°ããªã³ 8.6 ãœã«ãããŒã« 74.2 ã»ãªã³ 46.5 ã°ãªã»ãªã³ 83.8 å®æœäŸ ïŒ ãã¹ãã¢ããžã«ã³ãªã³å«æéçŽ90ïŒ ã®å€§è±ã¬ã·
ãã³2.5ïœããã¹ãã¢ããžã«åºå容äœãšããŠã®ã°
ãªã»ãªã³30mlãPH5.5ã®ç·©è¡æ¶²40mlãæŸç·èç±æ¥
ã®ãã¹ããªããŒãŒïŒ€æº¶æ¶²ïŒmlããã³ãã®æŽ»æ§åå€
ãšããŠã®ææ©ååç©ãæ··åãã50âã§20åéåå¿
ãããã äžèšè£œæ³ã«ãããŠãææ©ååç©ã®çš®é¡ããã³æ·»
å éãçš®ã å€æŽãããã¹ãã¢ããžã«åºç§»è»¢åå¿ç
æç©ã§ãããã¹ãã¢ããžã«ã°ãªã»ããŒã«ã®åçã
調ã¹ãããã®çµæãäžèšïŒ¡çŸ€ã®ååç©ãçšããå Ž
åã¯ãå®éšããç¯å²å ïŒææ©ååç©æ·»å é10ã
400mlïŒã®ããããã®æ·»å æ°Žæºã«ãããŠ20ïŒ ãã
ããåçãéæããããŸã矀ã®ååç©ã®å Žåã
10ã20ïŒ ã®åçãéæãããã ïŒã€ãœãããã«ãšãŒãã«ãïŒâãã³ã¿ãã³ãïŒ
âãã³ã¿ãã³ãã¡ãã«ã€ãœããã«ã±ãã³ãïŒâ
ããã¿ãã³ããžã€ãœããã«ã±ãã³ãã®é žã¡ã
ã«ãã®é žãšãã«ãé ¢é žãšãã«ãé ¢é žïœâããã
ã«ãé ¢é žã€ãœãããã«ãé ¢é žïœâããã«ãé ¢é ž
ã€ãœããã«ãé ¢é žsecâããã«ãé ¢é žïœâã¢ã
ã«ãé ¢é žã€ãœã¢ãã«ãé ¢é žïœâãã·ã«ãããã
ãªã³é žãšãã«ãé ªé žãšãã«ãã€ãœé ªé žãšãã«ã
åèé žãšãã«ãã€ãœåèé žãšãã«ãããããªã³
é žïœâãããã«ãé ªé žïœâããã«ããã³ãŒã³ã
ãã«ãšã³ããã·ã¬ã³ããžã¯ãã«ãšã¿ã³ãããšã
ã«ã¡ã«ã«ãã¿ã³ ïŒãšãã«ãšãŒãã«ãããã«ãšãŒãã«ãããã©ã
ãããã©ã³ããžãªããµã³ãã¢ã»ãã«ã¢ã»ãã³ã
ãªã¯ã¿ã³é žãšãã«ãããã«é žãžãšãã«ããã¬ã€
ã³é žãžãšãã«ãã³ãã¯é žãžãšãã«ãé ç³é žãžãš
ãã«ãã¯ãšã³é žããªãšãã«ãã€ãœåèé žã€ãœã¢
ãã«ããã³ã¿ã³ããããµã³ãããã¿ã³ãã€ãœãª
ã¯ã¿ã³ãã·ã¯ããããµã³ããžã¯ãã«ã¡ã¿ã³ãã
ãªã¯ãã«ã¡ã¿ã³ãããã©ã¯ãã«ã¡ã¿ã³ å®æœäŸ ïŒ ãã¹ãã¢ããžã«ã³ãªã³çŽ85ïŒ ãå«ãåµé»ã¬ã·ã
ã³ïŒmgã50ÎŒã®é ¢é žãšãã«ã«æº¶è§£ããã°ãªã»ã
ãŒã«5ÎŒã100mMã°ãªã·ã³ç·©è¡æ¶²ïŒPHïŒïŒ5ÎŒã
ããã³ãã€ããç±æ¥ã®ãã¹ããªããŒãŒïŒ€ïŒã·ã°ã
瀟補ïŒ0.8unitã®æ°Žæº¶æ¶²20ÎŒãå ïŒãŠã26âã§30
åéåå¿ãããã åå¿æ¶²ãã¯ãããã«ã æœåºããŠåŸããããªã³è
質ç»åã®çµæãèå±€ã¯ãããã°ã©ãã€ãŒã§èª¿ã¹ã
ãšããã36.5ïŒ ã®åçã§ãã¹ãã¢ããžã«ã°ãªã»ã
ãŒã«ãçæããŠããã å®æœäŸ ïŒ é ¢é žãšãã«ã®ä»£ããã«ãžãšãã«ãšãŒãã«ãçšã
ãã»ãã¯å®æœäŸïŒãšåæ§ã«ããŠåµé»ã¬ã·ãã³ãšã°
ãªã»ããŒã«ãåå¿ããããšããã39.2ïŒ ã®åçã§
ãã¹ãã¢ããžã«ã°ãªã»ããŒã«ãåŸãããã çºæã®å¹æ ãã¹ããªããŒãŒïŒ€ã®ãã¹ãã¢ããžã«åºè»¢ç§»åå¿
掻æ§ãïŒäŸ¡éå±ã€ãªã³ã«ãããã«ææ©ååç©ã§çº
çŸãããæ¬çºæã®è£œæ³ã¯ãåå¿ç³»ã«ïŒäŸ¡éå±ã€ãª
ã³ãå ¥ã蟌ãŸãªãããšã«ãããªã³é žå¡©ç·©è¡æ¶²ã®äœ¿
çšãèªç±ã«ãªãããã¹ããªããŒãŒïŒ€ãåå¿æ¡ä»¶ã®
éžæã®å¹ ãåºããªã€ãŠå®¹æã«æé©æ¡ä»¶ã§ã®åå¿ã
è¡ãããããšãã§ããããã«ãªãã»ããåå¿æ¶²ã
ãã®ç®çç©ã®åé¢ã»ç²Ÿè£œã容æã§ããããŸããïŒ
䟡éå±ã€ãªã³ã転移åå¿ãçŽæ¥åŠšå®³ããããã«åŸ
æ¥ã¯äœ¿çšäžå¯èœã§ãã€ããããªãªã³è質ãã¢ã«ã³
ãŒã«æ§æ°Žé žåºå«æååç©ãåå¿åæã«ããããšã
ã§ãããããæ¬çºæã«ããæ°èŠãªãã¹ãã¢ããžã³
é žèªå°äœãæäŸããéãéããããšã«ãªãã
ã¯ç³ãŸãã¯ã¢ã«ã³ãŒã«æ§æ°Žé žåºå«æå¡©åºã®æ°Žé žåº
éšåã®æ°ŽçŽ ååïŒåãé€ããåŸã«æ®ãææ©åºã
X2ã¯çŽéèèªæå€äŸ¡ã¢ã«ã³ãŒã«ãç³ãªã©ã®æ°Žé ž
åºå«æååç©ãããã®æ°Žé žåºéšåã®æ°ŽçŽ ååïŒå
ãé€ããåŸã«æ®ãææ©åºãè¡šãããïŒ ïŒäŸ¡éå±ã€ãªã³ã¯ããã¹ããªããŒãŒïŒ€ã«ãã¹ã
ã¢ããžã«åºè»¢ç§»åå¿æŽ»æ§ãçºçŸãããã®ã«äžå¯æ¬
ã®ãã®ãšèããããŠããïŒäœãããã¹ãã¢ããžã«
ã°ãªã»ããŒã«ãšããåäžãªã³è質ååéã§ã®ãã¹
ãã¢ããžã«åºè»¢ç§»åå¿ã«ãããŠã¯äŸå€çã«ïŒäŸ¡é
å±ã€ãªã³ã®æ·»å ãå¿ èŠãšããªãã€ããšããå ±åâ
Can.J.Biochem.ã51ã747ã1973âãããïŒãã
ããã€ãŠãåŸæ¥ãäžè¬çãªç°çš®ååéãã¹ãã¢ã
ãžã«åºè»¢ç§»åå¿ã«ãããã¹ãã¢ããžã³é žèªå°äœã
補é ããå Žåã¯å¿ ãïŒäŸ¡éå±ã€ãªã³ïŒæ®éã¯Ca
ã€ãªã³ïŒã«ãã€ãŠãã¹ããªããŒãŒïŒ€ã®æŽ»æ§åãè¡
ãªã€ãŠããïŒç¹éæ58â63388å·ãå58â67183
å·ãå59â187792å·ãå62â36195å·çïŒã ãã¹ããªããŒãŒïŒ€ã®æŽ»æ§åã«Caã€ãªã³ãçšã
ãå Žåãåå¿ç³»ã«ã¯ã€ãªã³ãšåå¿æ§ããç©è³ªãå
ããããšãã§ããªããããŸããæ°Žé žåºå«æååç©
ã§ãã€ãŠãCaã€ãªã³ãšååããŠåå¿æ§ã倱ãã
ããªãã®ã¯ãã¹ãã¢ããžã«åºå容äœãšããŠäœ¿ãã
ãšãã§ããªããããšãã°ã転移åå¿ã®è³é©PHãïŒ
ãïŒä»è¿ã«ããé µçŽ ãçšããå Žåãæã䜿ãæã
äžã€å®äŸ¡ãªãªã³é žç·©è¡æ¶²ã¯Caã€ãªã³ãšåå¿ããŠ
æ²æ®¿ãçãããã䜿ãããšãã§ãããããé«äŸ¡ãª
ç·©è¡æ¶²ã䜿çšããããåŸãªãããŸããç®çç©ã®äž
éšã«Caå¡©åã®ãã®ãã§ãããããNaå¡©åãªã©ä»
ã®å¡©åã®ãã®ã補é ããå Žåã¯ãã¬ãŒãå€ãçšã
ããããŠç®çãšããå¡©ã«å€æãããªã©ã®æäœãå¿
èŠã§ããã çºæã解決ããããšããåé¡ç¹ æ¬çºæã®ç®çã¯ããªã³è質ãšãã¹ããªããŒãŒïŒ€
ãçšããåŸæ¥ã®ãã¹ãã¢ããžã³é žèªå°äœã®è£œæ³ã
äžè¿°ã®ããã«ïŒäŸ¡éå±ã€ãªã³ã®äœ¿çšã«ãšããªã
çš®ã ã®å¶çŽãããã®ã§ãã€ãããšã«éã¿ãïŒäŸ¡é
å±ã€ãªã³ãçšããªããã¹ããªããŒãŒïŒ€ã®æŽ»æ§åæ³
ã«ãã補æ³ãæäŸããŠããã¹ãã¢ããžã³é žèªå°äœ
ã®è£œé ãåŸæ¥ããã容æã«ããããšã«ããã åé¡ç¹ã解決ããããã®æ段 äžèšç®çãéæããããšã«æåããæ¬çºæã®è£œ
æ³ã¯ããã¹ããªããŒãŒïŒ€ã®æŽ»æ§åã«ïŒäŸ¡éå±ã€ãª
ã³ãçšããããããã«ççŽ æ°ïŒã12ã®ãšã¹ãã«
é¡ãççŽ æ°ïŒãïŒã®ã±ãã³é¡ãççŽ æ°ïŒãïŒã®ç
åæ°ŽçŽ ãççŽ æ°ïŒãïŒã®ããã²ã³åçåæ°ŽçŽ ãç
çŽ æ°ïŒãïŒã®èèªæãšãŒãã«é¡ãããã¯ç°ç¶ãšãŒ
ãã«é¡ããŸãã¯ããšãã«ã¡ã«ã«ãã¿ã³ãçšããŠæŽ»
æ§åãè¡ãããšãç¹åŸŽãšãããã®ã§ããã ææ©æº¶åªããšãã°ãžãšãã«ãšãŒãã«ã¯ãåŸæ¥ã®
補æ³ã«ãããŠããã°ãã°åå¿ç³»æ§ææåãšããŠçš
ããããŠãããããããªãããåŸæ¥ã®è£œæ³ã«ãã
ãŠã¯ãææ©æº¶åªã¯æ°Žã«é£æº¶æ§ã®ãªã³è質ã溶ãã
ãŠé µçŽ ããã³ãã¹ãã¢ããžã«åºå容äœã®æ°Žæº¶æ¶²ãš
æ¥è§Šãããããã®åãªã溶åªãšããŠäœ¿ãããŠã
ããé µçŽ ã®æŽ»æ§åã«ã¯å¿ ãCaã€ãªã³çã®ïŒäŸ¡é
å±ã€ãªã³ãå¥ã«äœ¿ãããŠããã ïŒäŸ¡éå±ã€ãªã³ãçšããææ©ååç©ã«ããé µçŽ
掻æ§åãè¡ããã®çºæã¯ãæ¬çºæè ãã«ãã次ã®
ãããªæ°èŠãªç¥èŠã«åºã¥ããã®ã§ããã (a) ããçš®ã®ææ©ååç©ïŒããšãã°é ¢é žãšãã«ã
ïŒâããã¿ãã³çïŒã¯ãåå¿ç³»ã«ããæ¯ç以äž
ã«å ããããå ŽåãïŒäŸ¡éå±ã€ãªã³ãååšããª
ããŠããã¹ããªããŒãŒïŒ€ã掻æ§åããã (b) ããçš®ã®ææ©ååç©ïŒããšãã°äœçŽèèªæäž
çŽã¢ã«ã³ãŒã«ããžã¡ãã«ãã«ã ã¢ããããžã¡ã
ã«ã¹ã«ããã·ãçïŒã¯ããã¹ããªããŒãŒïŒ€ãã»
ãšãã©æŽ»æ§åãããããã倱掻ãããã (c) ãã¹ããªããŒãŒïŒ€ã掻æ§åããååç©ã®äžã«
ããããæ¿åºŠä»¥äžã§ã¯ãã¹ããªããŒãŒïŒ€ã倱掻
ããããã®ãå€ãïŒããšãã°åå¡©åççŽ ãïœâ
ãããµã³ããã³ãŒã³ãïŒâãã¿ããŒã«ããžãªã
ãµã³çïŒã (d) ãã¹ããªããŒãŒïŒ€ã«å¯ŸããŠäºå®äžäžæŽ»æ§ãªæ
æ©ååç©ãããªãã¡ãã¹ããªããŒãŒïŒ€ã掻æ§å
ããããŸã倱掻ããããªãååç©ãããã ãããã€ãŠãæ¬çºæã«ãããŠã¯ããã¹ããªããŒ
ãŒïŒ€ã«ãã¹ãã¢ããžã«åºè»¢ç§»åå¿æŽ»æ§ãçºçŸãã
ãäœçšãæããææ©ååç©ããäžèšæŽ»æ§åäœçšã®
ã»ããé µçŽ å€±æŽ»äœçšããã匷ãçŸããããããªæ¡
件ã§çšããŠããã¹ããªããŒãŒïŒ€ã掻æ§åããã é©é䜿çšããå Žåã«ãã¹ããªããŒãŒïŒ€ã掻æ§å
ãããšãšãã«ãªã³è質ã溶解ãããã¯åæ£ããã
èœåã®ç¹ã§ããããããããã€ãŠæ¬çºæã®è£œæ³ã«
ãããŠäœ¿çšããé µçŽ æŽ»æ§åçšææ©ååç©ãšããŠå¥œ
ãŸãããã®ã®å ·äœäŸã瀺ããšã次ã®ãããªãã®ã
ããã ãšã¹ãã«é¡ïŒççŽ æ°ïŒã10ã®èèªæã«ã«ãã³é ž
ããã³åãççŽ æ°ç¯å²ã®èèªæã¢ã«ã³ãŒã«ããåŸ
ãããççŽ æ°ïŒã12ã®ãšã¹ãã«é¡ãç¹ã«å¥œãŸã
ããããšãã°é ¢é žãšãã«ãé ¢é žããã«ãé ¢é žã¢ã
ã«ãé ¢é žãã·ã«ãããããªã³é žãšãã«ãããããª
ã³é žãããã«ãé ªé žãšãã«ãé ªé žããã«ãã€ãœé ª
é žã€ãœããã«ãã€ãœåèé žãšãã«ãªã©ã§ããããª
ãã§ãé ¢é žãšãã«ã¯ãïŒäŸ¡éå±å¡©ãçšããåŸæ¥ã®
補æ³ã§ã¯ãšããŠãäžå¯èœã§ãã€ããããªé«çïŒçŽ
90ïŒ ä»¥äžïŒã§ãã¹ãã¢ããžã«åºè»¢ç§»åå¿ãçèµ·ã
ãããã¹ãã¢ããžã³é žã®å¹ æãå°ãªãã ãã§ãª
ããåæ±ãã容æã§äŸ¡æ Œãäœå»ã§ãããããæ¬çº
æã®è£œæ³ã§çšãããã¹ããªããŒãŒïŒ€æŽ»æ§åå€ãšã
ãŠæã奜ãŸãããã®é žã¡ãã«ãã®é žãšãã«ã¯é µçŽ
倱掻äœçšããã匷ãã䜿ãé£ããççŽ æ°11以äžã®
é«çŽèèªæã«ã«ãã³é žãã¢ã«ã³ãŒã«ããåŸããã
ãšã¹ãã«ããããã¯ããããã·ã«ã«ãã³é žé¡ïŒã
ãšãã°ä¹³é žãããã«é žãã¯ãšã³é žçïŒã®ãšã¹ãã«
ã¯ããã¹ããªããŒãŒïŒ€æŽ»æ§åäœçšã匱ãã ã±ãã³é¡ïŒççŽ æ°ïŒãïŒã®èèªå±ã±ãã³ãããš
ãã°ïŒâãã³ã¿ãã³ãã¡ãã«ã€ãœããã«ã±ãã³ã
ïŒâããã¿ãã³ããžã€ãœããã«ã±ãã³ããç¹ã«å¥œ
ãŸãããããããã³ããã¿ãã³ãªã©ãççŽ æ°ãïŒ
æªæºã®ãã®ã¯ãé µçŽ å€±æŽ»äœçšã匷ãã ããã²ã³åçåæ°ŽçŽ ïŒãžã¯ãã«ã¡ã¿ã³ãããªã¯
ãã«ã¡ã¿ã³ããžã¯ãã«ãšã¿ã³çã奜ãŸããã åè¿°ã®ããã«ãããã奜ãŸããææ©ååç©ãšã
ãã©ã䜿çšéã«ãã€ãŠã¯é µçŽ ã倱掻ãããããšã
ããããŸã倱掻äœçšã匷ãçŸããæ¿åºŠããããã
ç°ãªãããã奜é©äœ¿çšéã¯ååç©ã«ãã€ãŠç°ãªã
ããå€ãã®å Žåã奜é©äœ¿çšéã¯æ°ŽïŒå®¹ééšã«å¯Ÿã
ãŠé µçŽ 掻æ§åçšææ©ååç©0.05ã50容ééšã®ç¯å²
ã«ãããåã ã®ææ©ååç©ã«ã€ããŠã®æé©äœ¿çšé
ã¯ãå®éšã«ãã容æã«ç¢ºèªããããšãã§ããã æ¬çºæã®è£œæ³ã«ãããŠãåæã®ãªã³è質ãšããŠ
ã¯ã°ãªã»ããªã³è質ã§ããã°ãããªããªã³è質ã
䜿çšããããšãã§ããããšãã°å€§è±ã¬ã·ãã³ãåµ
é»ã¬ã·ãã³ãªã©å€©ç¶ã®ãã®ã®ã»ãããã¹ãã¢ããž
ã«ã³ã³ããã¹ãã¢ããžã«ãšã¿ããŒã«ã¢ãã³ãªã©ã®
åæåãçšããããšãã§ããã ããŸäžã€ã®åå¿åæã§ããâãã¹ãã¢ããžã«åº
å容äœâãšãªãåŸãååç©ã¯ãæ°Žé žåºãæããå
åç©ãããªãã¡ã¡ã¿ããŒã«ããšã¿ããŒã«ãããã
ããŒã«ãïŒâãããããŒã«ããã¿ããŒã«ãïŒâã
ã¿ããŒã«ãã€ãœã¢ãã«ã¢ã«ã³ãŒã«ããããµããŒ
ã«ãããã¿ããŒã«ããªã¯ã¿ããŒã«ããã«ããŒã«ã
ã°ãªã»ãªã³ããšãã¬ã³ã°ãªã³ãŒã«çã®ã¢ã«ã³ãŒã«
é¡ïŒã°ãªã³ãŒã¹ããã·ããŒã¹ãã¢ã©ãããŒã¹ãã
ã«ã¯ããŒã¹ãβââã°ãªã³ãŒã¹ããã³ããŒã¹ã
ã¹ã¯ããŒã¹ãã©ã¯ããŒã¹ãã»ãããªãŒã¹ãã¡ãªã
ãªãŒã¹çã®ç³é¡ïŒã°ã«ã³ãµãã³ãã¬ã©ã¯ããµã
ã³ãã¢ã»ãã«ã¬ã©ã¯ããµãã³çã®ã¢ããç³ïŒãœã«
ãããŒã«ããã³ãããŒã«çã®ã¢ã«ã³ãŒã«ïŒã¢ãã°
ããªã³ããµãªã·ã³çã®é ç³äœïŒããšããŒã«ããã
ãããšããŒã«ããžãããããšããŒã«çã®ããšããŒ
ã«é¡ïŒãã®ä»ïŒããšãã°ã¢ããã·ã³ïŒã®äžããä»»
æã«éžã°ããã ãªããæ¬çºæã®ç®çã¯åå¿æ¹æ³ã®æ¹è¯ã«ããã
ç¹å®ã®ãã¹ãã¢ããžã³é žèªå°äœãæäŸããããšã«
ããã®ã§ã¯ãªãããããããªããªã³è質ãšãã¹ã
ã¢ããžã«åºå容äœãšãçµåããŠåå¿ããããã¯å š
ãèªç±ã§ããïŒæ¬çºæã®æ¹æ³ã«ãããŠã®ã¿ãã¹ã
ã¢ããžã«åºè»¢ç§»åå¿ãçãåŸãªããªã³è質ã¯èŠã
ã ãããŠããªãïŒã ãã¹ããªããŒãŒïŒ€ãšããŠã¯ããã€ããããã³ãž
ã³ãããŒãããªã©ã®æ€ç©äœããæœåºããããã®
ãšã埮çç©ç±æ¥ã®ãã®ãšããããæ¬çºæã«ãããŠ
ã¯ãã®ããããçšããŠããããã埮çç©ãç¹ã«æŸ
ç·èç±æ¥ã®ãã¹ããªããŒãŒïŒ€ã¯ãç±å®å®æ§ã«ãã
ãããšãšãã«æŽ»æ§ã匷ãã®ã§å¥œãŸãããæ¬çºæã®
補æ³ã«ãããŠããã¹ããªããŒãŒïŒ€ã¯åå¿ç³»ã«æº¶è§£
ãããŠçšããã»ããåºå®åé µçŽ ã®åœ¢ã§çšããŠãã
ãã ãªã³è質ããã¹ãã¢ããžã«åºå容äœãšãªãåå
ç©ããã¹ããªããŒãŒïŒ€ããã³ãã®æŽ»æ§åå€ãšãªã
ææ©ååç©ãæ··åããŠãã¹ãã¢ããžã«åºè»¢ç§»åå¿
ãçèµ·ãããå Žåãæ··åé ã¯ç¹ã«éå®ãããªã
ããäœè¡šçãªæ¹æ³ã¯æ¬¡ã®ãšããã§ããã  ãªã³è質ãæ°Žäžã«åæ£ãæžæ¿ãããããã«ã
ã¹ãã¢ããžã«åºå容äœãšãªãååç©ããã¹ããª
ããŒãŒïŒ€æº¶æ¶²ããã³é µçŽ 掻æ§åå€ãšããææ©å
åç©ãå ããŠæªæãæ··åããã  é µçŽ æŽ»æ§åå€ãšããææ©ååç©ã«ãªã³è質ã
溶解ãããã¹ãã¢ããžã«åºå容äœãšãªãååç©
ããã³ãã¹ããªããŒãŒïŒ€ã®æ°Žæº¶æ¶²ãšæ··åããã  é µçŽ æŽ»æ§åå€ãšããææ©ååç©ã«ãªã³è質ã
ãã³ãã¹ãã¢ããžã«åºå容äœãšãªãååç©ã溶
解ãããã¹ããªããŒãŒïŒ€ã®æ°Žæº¶æ¶²ãšæ··åããã åå¿ç³»ã«ã¯ãå¿ èŠã«å¿ããŠä»ã«ä»»æã®è£å©çæ
åãããšãã°PH調æŽã®ããç·©è¡å€ããªã³è質ãŸã
ã¯ãã®æº¶æ¶²ãåæ£ãããããã®çé¢æŽ»æ§å€çãã
é µçŽ äœçšã®åŠšããšãªããªãç¯å²ã§å ããããšãã§
ãããäœãïŒäŸ¡éå±ã€ãªã³ã¯ãææ©ååç©ã«ãã
ãã¹ããªããŒãŒïŒ€æŽ»æ§åã®åŠšããšãªãããã§ã¯ãª
ãããæ¬çºæã§ã¯äœ¿çšããªãã ãã¹ããªããŒãŒïŒ€ãåºå®åé µçŽ ã®åœ¢ã§çšããå Ž
åã¯ãåå¿ç³»ãæªæäžèŠã®å®å®ãªåäžçžã«ããŠã
ãããšãæãŸããããã®å Žåã¯ãåå¿ç³»æ§ææå
ãç¹å®ã®æ¯çã§é åããããšãã«åœ¢æãããéæ
ãªåäžçžãåºå®åé µçŽ ãšæ¥è§Šããããšãããäžèš
éæãªåäžçžã¯ãé µçŽ æŽ»æ§åçšã®ææ©ååç©ã«ãª
ã³è質ãæ¯èŒçé«æ¿åºŠã«æº¶è§£ããããã«ãã¹ãã¢
ããžã«åºå容äœãšãªãååç©ã®æ°Žæº¶æ¶²ãäžå®ã®é
床å ã§å ããŠæªæãããšããªã³è質ã®çé¢æŽ»æ§ã
é¢äžããŠåœ¢æãããããã®åäžçžã¯ãé µçŽ æŽ»æ§å
å€ãšããŠã®ææ©ååç©ä»¥å€ã®èŠªæ°Žæ§æº¶åªãå°éå
ãããšåœ¢æããæããªãããšããããããšãã°ã
åµé»ã¬ã·ãã³20ééïŒ ãã°ãªã»ãªã³ïŒééïŒ ãé ¢
é žãšãã«60ééïŒ ãïŒâãããããŒã«ïŒééïŒ ã
æ°Ž10ééïŒ ã®çµæã®æ··åç©ã¯ã容æã«ãéæãªå
äžçžã圢æããã çšããé µçŽ ã®è³é©æž©åºŠä»è¿ã§å åæéåå¿ãã
ãã®ã¡ãåå¿æ¶²ããã¢ã»ãã³ããšã¿ããŒã«ãçšã
ã溶åªåå¥æ³ãã·ãªã«ã²ã«ãã¢ã«ãããããã¯é
çžæ äœçãçšããã«ã©ã ã¯ãããã°ã©ãã€ãŒãè
çžã¯ãããã°ã©ãã€ãŒçã®æ¹æ³ã«ãããç®çãšã
ããã¹ãã¢ããžã³é žèªå°äœãååããããšãã§ã
ãã å®æœäŸ 以äžãå®æœäŸã瀺ããŠæ¬çºæã説æããã å®æœäŸ ïŒ åµé»ã¬ã·ãã³ãã調補ããããã¹ãã¢ããžã«ã³
ãªã³ïŒmgïŒã·ã°ã瀟ïŒã«ããã¹ãã¢ããžã«åºå容
äœãšããŠã°ãªã»ãªã³100ÎŒãPH6.0ã®ãªã³é žç·©è¡
液400ÎŒãæŸç·èç±æ¥ã®ãã¹ããªããŒãŒD1ãŠã
ãããå ããæ··åããã äžèšã®ããã«ããŠåŸãããæ··åç©ã«ãïŒã
100ÎŒã®é ¢é žãšãã«ãå ãã50âã«ä¿ã€ãŠæ¯ãš
ãããªããããã¹ãã¢ããžã«åºè»¢ç§»åå¿ã«ããã
ã¹ãã¢ããžã«ã°ãªã»ããŒã«ã®çæéãçµæçã«å®
éããããã®çµæã¯è¡šïŒã®ãšããã§ãã€ãŠãïŒäŸ¡
éå±ã€ãªã³ãå ããªããŠããé ¢é žãšãã«æ·»å é
30Ό以äžã®å Žåã«ãã¹ãã¢ããžã«ã°ãªã»ããŒã«
ãçæããã è¡šïŒ ãã¹ãã¢ããžã«ã°ãªã»ããŒã«çæéåºŠé ¢é žãšãã«æ·»å çæé床 ïŒÎŒïŒ ïŒÎŒgïŒunitã»minïŒ ïŒ ïŒ 10 ïŒ 30 35 50 124 75 143 100 101 å®æœäŸ ïŒ ãã¹ãã¢ããžã«ã³ãªã³å«æéãçŽ90ïŒ ã§ãã倧
è±ã¬ã·ãã³2.5ïœãæ¿åºŠ30ïŒ ïŒïœïŒïœïŒã®ãã¹ã
ã¢ããžã«åºå容äœæ°Žæº¶æ¶²100mlãæŸç·èç±æ¥ã®ã
ã¹ããªããŒãŒïŒ€æº¶æ¶²ïŒmlãé ¢é žãšãã«375mlãæ··
åããæªæããªãã50âã§60åéåå¿ãããã å容äœãçš®ã å€æŽããŠäžèšè£œé æ³ãå®æœããçµ
æãè¡šïŒã«ç€ºãããªããè¡šäžã®ãïŒ ãã¯ãåå¿æ¶²
äžã®æªåå¿ãªã³è質ãå«ãå šãã¹ãã¢ããžã³é žèª
å°äœåºæºã®ééïŒ ã§ããã è¡šïŒåå®¹äœ è»¢ç§»åå¿çæç©ïŒïŒ ïŒ ãã·ããŒã¹ 72.2 ã¬ã©ã¯ããŒã¹ 1.3 ã¢ãã°ããªã³ 8.6 ãœã«ãããŒã« 74.2 ã»ãªã³ 46.5 ã°ãªã»ãªã³ 83.8 å®æœäŸ ïŒ ãã¹ãã¢ããžã«ã³ãªã³å«æéçŽ90ïŒ ã®å€§è±ã¬ã·
ãã³2.5ïœããã¹ãã¢ããžã«åºå容äœãšããŠã®ã°
ãªã»ãªã³30mlãPH5.5ã®ç·©è¡æ¶²40mlãæŸç·èç±æ¥
ã®ãã¹ããªããŒãŒïŒ€æº¶æ¶²ïŒmlããã³ãã®æŽ»æ§åå€
ãšããŠã®ææ©ååç©ãæ··åãã50âã§20åéåå¿
ãããã äžèšè£œæ³ã«ãããŠãææ©ååç©ã®çš®é¡ããã³æ·»
å éãçš®ã å€æŽãããã¹ãã¢ããžã«åºç§»è»¢åå¿ç
æç©ã§ãããã¹ãã¢ããžã«ã°ãªã»ããŒã«ã®åçã
調ã¹ãããã®çµæãäžèšïŒ¡çŸ€ã®ååç©ãçšããå Ž
åã¯ãå®éšããç¯å²å ïŒææ©ååç©æ·»å é10ã
400mlïŒã®ããããã®æ·»å æ°Žæºã«ãããŠ20ïŒ ãã
ããåçãéæããããŸã矀ã®ååç©ã®å Žåã
10ã20ïŒ ã®åçãéæãããã ïŒã€ãœãããã«ãšãŒãã«ãïŒâãã³ã¿ãã³ãïŒ
âãã³ã¿ãã³ãã¡ãã«ã€ãœããã«ã±ãã³ãïŒâ
ããã¿ãã³ããžã€ãœããã«ã±ãã³ãã®é žã¡ã
ã«ãã®é žãšãã«ãé ¢é žãšãã«ãé ¢é žïœâããã
ã«ãé ¢é žã€ãœãããã«ãé ¢é žïœâããã«ãé ¢é ž
ã€ãœããã«ãé ¢é žsecâããã«ãé ¢é žïœâã¢ã
ã«ãé ¢é žã€ãœã¢ãã«ãé ¢é žïœâãã·ã«ãããã
ãªã³é žãšãã«ãé ªé žãšãã«ãã€ãœé ªé žãšãã«ã
åèé žãšãã«ãã€ãœåèé žãšãã«ãããããªã³
é žïœâãããã«ãé ªé žïœâããã«ããã³ãŒã³ã
ãã«ãšã³ããã·ã¬ã³ããžã¯ãã«ãšã¿ã³ãããšã
ã«ã¡ã«ã«ãã¿ã³ ïŒãšãã«ãšãŒãã«ãããã«ãšãŒãã«ãããã©ã
ãããã©ã³ããžãªããµã³ãã¢ã»ãã«ã¢ã»ãã³ã
ãªã¯ã¿ã³é žãšãã«ãããã«é žãžãšãã«ããã¬ã€
ã³é žãžãšãã«ãã³ãã¯é žãžãšãã«ãé ç³é žãžãš
ãã«ãã¯ãšã³é žããªãšãã«ãã€ãœåèé žã€ãœã¢
ãã«ããã³ã¿ã³ããããµã³ãããã¿ã³ãã€ãœãª
ã¯ã¿ã³ãã·ã¯ããããµã³ããžã¯ãã«ã¡ã¿ã³ãã
ãªã¯ãã«ã¡ã¿ã³ãããã©ã¯ãã«ã¡ã¿ã³ å®æœäŸ ïŒ ãã¹ãã¢ããžã«ã³ãªã³çŽ85ïŒ ãå«ãåµé»ã¬ã·ã
ã³ïŒmgã50ÎŒã®é ¢é žãšãã«ã«æº¶è§£ããã°ãªã»ã
ãŒã«5ÎŒã100mMã°ãªã·ã³ç·©è¡æ¶²ïŒPHïŒïŒ5ÎŒã
ããã³ãã€ããç±æ¥ã®ãã¹ããªããŒãŒïŒ€ïŒã·ã°ã
瀟補ïŒ0.8unitã®æ°Žæº¶æ¶²20ÎŒãå ïŒãŠã26âã§30
åéåå¿ãããã åå¿æ¶²ãã¯ãããã«ã æœåºããŠåŸããããªã³è
質ç»åã®çµæãèå±€ã¯ãããã°ã©ãã€ãŒã§èª¿ã¹ã
ãšããã36.5ïŒ ã®åçã§ãã¹ãã¢ããžã«ã°ãªã»ã
ãŒã«ãçæããŠããã å®æœäŸ ïŒ é ¢é žãšãã«ã®ä»£ããã«ãžãšãã«ãšãŒãã«ãçšã
ãã»ãã¯å®æœäŸïŒãšåæ§ã«ããŠåµé»ã¬ã·ãã³ãšã°
ãªã»ããŒã«ãåå¿ããããšããã39.2ïŒ ã®åçã§
ãã¹ãã¢ããžã«ã°ãªã»ããŒã«ãåŸãããã çºæã®å¹æ ãã¹ããªããŒãŒïŒ€ã®ãã¹ãã¢ããžã«åºè»¢ç§»åå¿
掻æ§ãïŒäŸ¡éå±ã€ãªã³ã«ãããã«ææ©ååç©ã§çº
çŸãããæ¬çºæã®è£œæ³ã¯ãåå¿ç³»ã«ïŒäŸ¡éå±ã€ãª
ã³ãå ¥ã蟌ãŸãªãããšã«ãããªã³é žå¡©ç·©è¡æ¶²ã®äœ¿
çšãèªç±ã«ãªãããã¹ããªããŒãŒïŒ€ãåå¿æ¡ä»¶ã®
éžæã®å¹ ãåºããªã€ãŠå®¹æã«æé©æ¡ä»¶ã§ã®åå¿ã
è¡ãããããšãã§ããããã«ãªãã»ããåå¿æ¶²ã
ãã®ç®çç©ã®åé¢ã»ç²Ÿè£œã容æã§ããããŸããïŒ
䟡éå±ã€ãªã³ã転移åå¿ãçŽæ¥åŠšå®³ããããã«åŸ
æ¥ã¯äœ¿çšäžå¯èœã§ãã€ããããªãªã³è質ãã¢ã«ã³
ãŒã«æ§æ°Žé žåºå«æååç©ãåå¿åæã«ããããšã
ã§ãããããæ¬çºæã«ããæ°èŠãªãã¹ãã¢ããžã³
é žèªå°äœãæäŸããéãéããããšã«ãªãã
Claims (1)
- ãç¹èš±è«æ±ã®ç¯å²ã ïŒ ãã¹ãã¢ããžã«åºå容äœã®ååšäžããªã³è質
ã«ãã¹ããªããŒãŒïŒ€ãäœçšãããŠãã¹ãã¢ããžã«
åºè»¢ç§»åå¿ãçèµ·ãããããšã«ãããã¹ãã¢ããž
ã³é žèªå°äœã補é ããã«åœãããã¹ããªããŒãŒïŒ€
ã«ãã¹ãã¢ããžã«åºè»¢ç§»åå¿æŽ»æ§ãçºçŸãããã
ãã®ïŒäŸ¡éå±ã€ãªã³ãçšãããççŽ æ°ïŒã12ã®ãš
ã¹ãã«é¡ãççŽ æ°ïŒãïŒã®ã±ãã³é¡ãççŽ æ°ïŒã
ïŒã®çåæ°ŽçŽ ãççŽ æ°ïŒãïŒã®ããã²ã³åçåæ°Ž
çŽ ãççŽ æ°ïŒãïŒã®èèªæãšãŒãã«é¡ãããã¯ç°
ç¶ãšãŒãã«é¡ããŸãã¯ããšãã«ã¡ã«ã«ãã¿ã³ã«ã
ããã¹ããªããŒãŒïŒ€ã®æŽ»æ§åãè¡ãããšãç¹åŸŽãš
ãããã¹ãã¢ããžã³é žèªå°äœã®è£œé æ³ã ïŒ ãã¹ããªããŒãŒïŒ€ã®æŽ»æ§åãé ¢é žãšãã«ã«ã
ãè¡ãç¹èš±è«æ±ã®ç¯å²ç¬¬ïŒé èšèŒã®è£œé æ³ã
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62075907A JPS63245684A (ja) | 1987-03-31 | 1987-03-31 | ãã¹ãã¢ããžã³é žèªå°äœã®è£œé æ³ |
| EP88302894A EP0285421B1 (en) | 1987-03-31 | 1988-03-30 | Method of producing phosphatidic acid derivatives |
| CA000562975A CA1324777C (en) | 1987-03-31 | 1988-03-30 | Method of producing phosphatidic acid derivatives |
| DE88302894T DE3881091T2 (de) | 1987-03-31 | 1988-03-30 | Verfahren zur herstellung von phosphatidsaeurederivaten. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62075907A JPS63245684A (ja) | 1987-03-31 | 1987-03-31 | ãã¹ãã¢ããžã³é žèªå°äœã®è£œé æ³ |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63245684A JPS63245684A (ja) | 1988-10-12 |
| JPH0573390B2 true JPH0573390B2 (ja) | 1993-10-14 |
Family
ID=13589877
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62075907A Granted JPS63245684A (ja) | 1987-03-31 | 1987-03-31 | ãã¹ãã¢ããžã³é žèªå°äœã®è£œé æ³ |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0285421B1 (ja) |
| JP (1) | JPS63245684A (ja) |
| CA (1) | CA1324777C (ja) |
| DE (1) | DE3881091T2 (ja) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011527566A (ja) * | 2008-07-08 | 2011-11-04 | ã±ã㌠ãœã·ãšã¿ ãã« ã¢ããªã | âã¢ã·ã«âãã¹ãã¡ããžã«âãšã¿ããŒã«ã¢ãã³ãçç£ããæ¹æ³ |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02273536A (ja) * | 1989-04-13 | 1990-11-08 | Yakult Honsha Co Ltd | çé¢æŽ»æ§å€ããã³ãã®è£œé æ³ |
| JP2877439B2 (ja) * | 1989-05-17 | 1999-03-31 | ååéé µå·¥æ¥æ ªåŒäŒç€Ÿ | åµã®æ¹è³ªæ¹æ³ |
| US5183750A (en) * | 1989-05-26 | 1993-02-02 | Kao Corporation | Processes for the production of phosphatidic acid |
| JPH07108201B2 (ja) * | 1991-01-09 | 1995-11-22 | åå€å±è£œé ªæ ªåŒäŒç€Ÿ | ãã€ããã¯ãªãŒã çµæç© |
| JP3053537B2 (ja) | 1994-11-08 | 2000-06-19 | æ ªåŒäŒç€Ÿã€ã¯ã«ãæ¬ç€Ÿ | è³æ©èœæ¹åå€ |
| JP3791951B2 (ja) * | 1995-11-08 | 2006-06-28 | æ ªåŒäŒç€Ÿã€ã¯ã«ãæ¬ç€Ÿ | å€äŸ¡äžé£œåèèªé žå«æãã¹ãã¡ããžã«ã»ãªã³ãå«ãæ²¹èçµæç©ã®è£œé æ¹æ³ |
| DE10142014B4 (de) | 2001-08-28 | 2004-11-11 | Degussa Bioactives Deutschland Gmbh & Co. Kg | Verfahren zur Herstellung von Phosphatidylserin |
| JP4678488B2 (ja) * | 2005-03-28 | 2011-04-27 | ãã¬ã»ã±ã ããã¯ã¹æ ªåŒäŒç€Ÿ | ãªã³è質ã®å æ°Žå解æ¹æ³åã³å¡©åºäº€ææ¹æ³ |
| ITPD20050164A1 (it) | 2005-05-30 | 2006-11-30 | Fidia Farmaceutici | Processo per la preparazione e l'isolamento di fosfatidi |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6041494A (ja) * | 1983-04-11 | 1985-03-05 | Meito Sangyo Kk | é µçŽ æ³ãªã³è質äžçŽã¢ã«ã³âã«èªå°äœã®è£œæ³ |
| JPS6336760A (ja) * | 1986-07-30 | 1988-02-17 | Yanagiya:Kk | 竹茪補é çšäž² |
| JPS6391089A (ja) * | 1986-10-03 | 1988-04-21 | Meito Sangyo Kk | ãªã³è質èªå°äœã®åºå®åé µçŽ æ³ã«ããè£œæ³ |
| JPS63123389A (ja) * | 1986-11-14 | 1988-05-27 | Meito Sangyo Kk | é µçŽ æ³ãªã³è質âïœâã»ãªã³èªå°äœã®è£œé æ³ |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59187792A (ja) * | 1983-04-11 | 1984-10-24 | Meito Sangyo Kk | é µçŽ æ³ãªã³è質ç³é¡èªå°äœã®è£œæ³ |
-
1987
- 1987-03-31 JP JP62075907A patent/JPS63245684A/ja active Granted
-
1988
- 1988-03-30 CA CA000562975A patent/CA1324777C/en not_active Expired - Fee Related
- 1988-03-30 DE DE88302894T patent/DE3881091T2/de not_active Expired - Fee Related
- 1988-03-30 EP EP88302894A patent/EP0285421B1/en not_active Expired - Lifetime
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6041494A (ja) * | 1983-04-11 | 1985-03-05 | Meito Sangyo Kk | é µçŽ æ³ãªã³è質äžçŽã¢ã«ã³âã«èªå°äœã®è£œæ³ |
| JPS6336760A (ja) * | 1986-07-30 | 1988-02-17 | Yanagiya:Kk | 竹茪補é çšäž² |
| JPS6391089A (ja) * | 1986-10-03 | 1988-04-21 | Meito Sangyo Kk | ãªã³è質èªå°äœã®åºå®åé µçŽ æ³ã«ããè£œæ³ |
| JPS63123389A (ja) * | 1986-11-14 | 1988-05-27 | Meito Sangyo Kk | é µçŽ æ³ãªã³è質âïœâã»ãªã³èªå°äœã®è£œé æ³ |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011527566A (ja) * | 2008-07-08 | 2011-11-04 | ã±ã㌠ãœã·ãšã¿ ãã« ã¢ããªã | âã¢ã·ã«âãã¹ãã¡ããžã«âãšã¿ããŒã«ã¢ãã³ãçç£ããæ¹æ³ |
Also Published As
| Publication number | Publication date |
|---|---|
| DE3881091D1 (de) | 1993-06-24 |
| CA1324777C (en) | 1993-11-30 |
| DE3881091T2 (de) | 1993-11-04 |
| EP0285421A2 (en) | 1988-10-05 |
| EP0285421B1 (en) | 1993-05-19 |
| JPS63245684A (ja) | 1988-10-12 |
| EP0285421A3 (en) | 1990-05-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Lennarz et al. | The chemical characterization and enzymatic synthesis of mannolipids in Micrococcus lysodeikticus | |
| EP0125039B1 (en) | Enzymatic production of phospholipid-saccharide derivatives | |
| JPH0573390B2 (ja) | ||
| Vance et al. | Purification and properties of the fatty acid synthetase from Mycobacterium phlei | |
| EP1412511B1 (en) | Process for the production of phospholipids | |
| EP0122152B1 (en) | Enzymatic production of sphingophospholipid derivatives | |
| Veerkamp et al. | Biochemical changes in Bifidobacterium bifidum var. Pennsylvanicus after cell wall inhibition VII. Structure of the phosphogalactolipids | |
| Okahata et al. | Simple transphosphatidylation of phospholipids catalysed by a lipid-coated phospholipase D in organic solvents | |
| Savel'ev et al. | Enzymatic properties of α-D-galactosidase from Trichoderma reesei | |
| US6660504B2 (en) | Process for exchanging bases in phospholipids | |
| Han et al. | Lipase-catalyzed synthesis of lysophosphatidic acid in a solvent free system | |
| Manners et al. | Studies on carbohydrate metabolizing enzymes: XVI. Specificity of laminaribiose phosphorylase from Astasia ocellata | |
| Takagi et al. | Function of the phosphate group of pyridoxal 5'-phosphate in the glycogen phosphorylase reaction. | |
| JPH03123493A (ja) | ãžã¢ã·ã«ã°ãªã»ããªã³è質ã®å æ°Žåè§£æ³ | |
| Fukunaga et al. | Preparation of the gemini detergent-lipase complexes and their high enzymatic activities in the transesterifications in homogeneous organic solvents | |
| JPS63245685A (ja) | ãªã³è質ã«ãã¹ããªããŒãŒãäœçšãããæ¹æ³ | |
| EP0137601B1 (en) | Enzymatic synthesis of gallic acid esters | |
| Aurich et al. | Production of octadecylphospho-L-serine by phospholipase D | |
| JPS63191802A (ja) | ãµã€ã¯ãããã¹ããªã³é¡èèªé žãšã¹ãã«ã®è£œé æ³ | |
| JPH08243374A (ja) | ç³è質åçé¢æŽ»æ§å€ | |
| KR0167489B1 (ko) | ë²€ì§ ë° íë ìí-ë-êžë£šìœíŒëŒë žì¬ìŽë ì ë첎ì ì ì¡°ë°©ë² | |
| JP2706941B2 (ja) | ãªããŒãŒé¡ã®å®å®åå€ | |
| JPH0542917B2 (ja) | ||
| Alexander | Comparative studies of yeast and sugarcane invertases | |
| JP2886626B2 (ja) | ãžã¢ã·ã«ã°ãªã»ããªã³è質ã®è£œé æ¹æ³ |