JP7405086B2 - ヘアピン型一本鎖ｒｎａ分子の製造方法 - Google Patents

ヘアピン型一本鎖ｒｎａ分子の製造方法 Download PDF

Info

Publication number
JP7405086B2
JP7405086B2 JP2020550483A JP2020550483A JP7405086B2 JP 7405086 B2 JP7405086 B2 JP 7405086B2 JP 2020550483 A JP2020550483 A JP 2020550483A JP 2020550483 A JP2020550483 A JP 2020550483A JP 7405086 B2 JP7405086 B2 JP 7405086B2
Authority
JP
Japan
Prior art keywords
dehydration condensation
condensation agent
rna molecule
group
formula
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2020550483A
Other languages
English (en)
Japanese (ja)
Other versions
JPWO2020071407A1 (ja
JPWO2020071407A5 (enExample
Inventor
恭平 越本
克彦 伊関
英朗 稲田
達也 藤田
慶一 沖村
崇隆 國嶋
忠明 大木
絵里子 青木
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toray Industries Inc
Original Assignee
Toray Industries Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toray Industries Inc filed Critical Toray Industries Inc
Publication of JPWO2020071407A1 publication Critical patent/JPWO2020071407A1/ja
Publication of JPWO2020071407A5 publication Critical patent/JPWO2020071407A5/ja
Application granted granted Critical
Publication of JP7405086B2 publication Critical patent/JP7405086B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1136—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against growth factors, growth regulators, cytokines, lymphokines or hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Endocrinology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
JP2020550483A 2018-10-02 2019-10-02 ヘアピン型一本鎖ｒｎａ分子の製造方法 Active JP7405086B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2018187767 2018-10-02
JP2018187767 2018-10-02
PCT/JP2019/038860 WO2020071407A1 (ja) 2018-10-02 2019-10-02 ヘアピン型一本鎖ｒｎａ分子の製造方法

Publications (3)

Publication Number Publication Date
JPWO2020071407A1 JPWO2020071407A1 (ja) 2021-09-02
JPWO2020071407A5 JPWO2020071407A5 (enExample) 2022-10-04
JP7405086B2 true JP7405086B2 (ja) 2023-12-26

Family

ID=70055619

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2020550483A Active JP7405086B2 (ja) 2018-10-02 2019-10-02 ヘアピン型一本鎖ｒｎａ分子の製造方法

Country Status (8)

Country Link
US (2) US11891602B2 (enExample)
EP (1) EP3862431B1 (enExample)
JP (1) JP7405086B2 (enExample)
KR (1) KR20210069628A (enExample)
CN (1) CN112673102A (enExample)
ES (1) ES2981806T3 (enExample)
TW (1) TWI848981B (enExample)
WO (1) WO2020071407A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114685572B (zh) * 2022-06-02 2022-08-30 上海百力格生物技术有限公司 用于mgb核酸探针合成的氧化剂组合物及探针的合成方法
WO2024112876A2 (en) * 2022-11-23 2024-05-30 Prime Medicine, Inc. Split synthesis of long rnas
CN117143179B (zh) * 2023-09-18 2024-07-12 安徽峆一药业股份有限公司 一种凝血酶发色底物s-2238的合成方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013133221A1 (ja) 2012-03-04 2013-09-12 株式会社ボナック microRNA阻害剤
WO2017073767A1 (ja) 2015-10-30 2017-05-04 株式会社ボナック ＴＧＦ－β１遺伝子の発現を抑制する一本鎖核酸分子を安定に含有する組成物

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994007910A1 (en) 1992-09-28 1994-04-14 Research Corporation Technologies, Inc. New reagents for peptide couplings
DE60014542T2 (de) 1999-03-08 2006-03-09 Tokuyama Corp., Tokuyama Ein verfahren zur herstellung von carbonsäure-derivaten und ein kondensationshilfsmittel mit einem quarternären ammonium-salz
US6825347B2 (en) 2001-05-21 2004-11-30 Research Corporation Technologies, Inc. Uronium and immonium salts for peptide coupling
AU2003256857A1 (en) 2002-08-08 2004-02-25 Dharmacon, Inc. Short interfering rnas having a hairpin structure containing a non-nucleotide loop
JP5046002B2 (ja) 2007-03-02 2012-10-10 公益財団法人新産業創造研究機構 塩化トリアジン誘導体、その製造方法およびその使用
SG186899A1 (en) 2010-07-08 2013-02-28 Bonac Corp Single-strand nucleic acid molecule for controlling gene expression
ES2533129T3 (es) 2010-08-03 2015-04-07 Bonac Corporation Molécula de ARN monocatenario que tiene esqueleto alicíclico que contiene nitrógeno
WO2013103146A1 (ja) 2012-01-07 2013-07-11 株式会社ボナック アミノ酸骨格を有する一本鎖核酸分子
JP2013153736A (ja) 2012-01-07 2013-08-15 Bonac Corp ペプチド骨格を有する一本鎖核酸分子
WO2015093495A1 (ja) * 2013-12-16 2015-06-25 株式会社ボナック ＴＧＦ－β１遺伝子発現制御のための一本鎖核酸分子
JP6486700B2 (ja) 2015-01-29 2019-03-20 国立大学法人金沢大学 脱水縮合剤
JP6486699B2 (ja) 2015-01-29 2019-03-20 国立大学法人金沢大学 脱水縮合剤
WO2017035090A1 (en) 2015-08-21 2017-03-02 Thomas Jefferson University Dumbbell-pcr: a method to quantify specific small rna variants with a single nucleotide resolution at terminal sequences
JP2018187767A (ja) 2015-09-29 2018-11-29 綜研化学株式会社 撥水性部材及びその製造方法
JP6742606B2 (ja) 2016-02-25 2020-08-19 国立大学法人金沢大学 トリアジン含有ポリマー、その製造方法、及びその脱水縮合剤としての用途
US20210115485A1 (en) * 2018-03-30 2021-04-22 Sumitomo Chemical Company, Limited Method for producing single-strand rna
EP3778886A4 (en) * 2018-03-30 2022-11-02 Toray Industries, Inc. Method for producing hairpin single-stranded rna molecule

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013133221A1 (ja) 2012-03-04 2013-09-12 株式会社ボナック microRNA阻害剤
WO2017073767A1 (ja) 2015-10-30 2017-05-04 株式会社ボナック ＴＧＦ－β１遺伝子の発現を抑制する一本鎖核酸分子を安定に含有する組成物

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LIU, Y., et al.，Tetrahedron，2008年，Vol. 64, Issue 36，pp. 8417-8422

Also Published As

Publication number Publication date
JPWO2020071407A1 (ja) 2021-09-02
EP3862431A1 (en) 2021-08-11
EP3862431B1 (en) 2024-06-26
CN112673102A (zh) 2021-04-16
US11891602B2 (en) 2024-02-06
TWI848981B (zh) 2024-07-21
US20240110186A1 (en) 2024-04-04
US20210317495A1 (en) 2021-10-14
KR20210069628A (ko) 2021-06-11
WO2020071407A1 (ja) 2020-04-09
TW202022117A (zh) 2020-06-16
EP3862431A4 (en) 2023-05-10
ES2981806T3 (es) 2024-10-10

Similar Documents

Publication Publication Date Title
US20240110186A1 (en) Method of producing hairpin single-stranded rna molecules
JP6853193B2 (ja) 核酸複合体
JP7363316B2 (ja) ヘアピン型一本鎖ｒｎａ分子の製造方法
PL184920B1 (pl) Nowe analogi oligonukleotydówĆ sposób ich wytwarzania i środek farmaceutyczny
WO2012074038A1 (ja) 修飾１本鎖ポリヌクレオチド
TW202613310A (zh) 含有三氮唑結構的GalNAc化合物及其寡核苷酸綴合物
KR20240166519A (ko) 치료제의 표적화된 전달을 위한 비사이클릭 헤테로사이클 및 이의 리간드
Abramova et al. Solid-phase-supported synthesis of morpholinoglycine oligonucleotide mimics
JP2023063434A (ja) オリゴヌクレオチド誘導体またはその塩
CN116514795A (zh) 3CLpro蛋白酶抑制剂的制备方法
JP6429264B2 (ja) ボラノホスフェート化合物、及び核酸オリゴマー
JP7313619B2 (ja) 化合物、蛍光標識導入剤、及び蛍光標識導入方法
Efthymiou et al. Synthesis and properties of oligonucleotides that contain a triazole‐linked nucleic acid dimer
JP4805143B2 (ja) ２‘水酸基を修飾された新規人工ｒｎａ
HK40113968A (en) Galnac compound containing triazole structure and oligonucleotide conjugate thereof
HK40113968B (en) Galnac compound containing triazole structure and oligonucleotide conjugate thereof
CN120457206A (zh) 偶联用细胞膜透过性肽
HK40039901A (en) Method for producing hairpin single-stranded rna molecule
JP2021178790A (ja) 核酸の製造方法、およびシランカップリング処理用組成物
CA3108806A1 (en) Reagents based on a tertiary amine backbone to introduce chemical functionality in nucleic acids and sequence-controlled polymers
JPWO2007029364A1 (ja) ピロールアミド４量体を含む２’−デオキシグアノシンハイブリッド化合物及びその製造方法、そのための製造中間体
PL218817B1 (pl) Sposób wytwarzania oligorybonukleotydów zawierających 5-taurynometylo-urydynę lub 5-taurynometylo-2-tiourydynę oraz hipermodyfikowanych jednostek monomerycznych do syntezy tych oligorybonukleotydów

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220926

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20220926

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20230815

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20231012

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20231114

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20231127

R151 Written notification of patent or utility model registration

Ref document number: 7405086

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R151