CN112673102A - 发夹型单链rna分子的制备方法 - Google Patents

发夹型单链rna分子的制备方法 Download PDF

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Publication number
CN112673102A
CN112673102A CN201980061140.0A CN201980061140A CN112673102A CN 112673102 A CN112673102 A CN 112673102A CN 201980061140 A CN201980061140 A CN 201980061140A CN 112673102 A CN112673102 A CN 112673102A
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stranded
linker
condensing agent
formula
dehydration condensation
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Chinese (zh)
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越本恭平
伊关克彦
稻田英朗
藤田达也
冲村庆一
国嶋崇隆
大木忠明
青木绘里子
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Toray Industries Inc
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Toray Industries Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1136—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against growth factors, growth regulators, cytokines, lymphokines or hormones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised

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  • Health & Medical Sciences (AREA)
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  • Engineering & Computer Science (AREA)
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  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
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  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Endocrinology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
CN201980061140.0A 2018-10-02 2019-10-02 发夹型单链rna分子的制备方法 Pending CN112673102A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2018187767 2018-10-02
JP2018-187767 2018-10-02
PCT/JP2019/038860 WO2020071407A1 (ja) 2018-10-02 2019-10-02 ヘアピン型一本鎖ｒｎａ分子の製造方法

Publications (1)

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CN112673102A true CN112673102A (zh) 2021-04-16

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CN201980061140.0A Pending CN112673102A (zh) 2018-10-02 2019-10-02 发夹型单链rna分子的制备方法

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US (2) US11891602B2 (enExample)
EP (1) EP3862431B1 (enExample)
JP (1) JP7405086B2 (enExample)
KR (1) KR20210069628A (enExample)
CN (1) CN112673102A (enExample)
ES (1) ES2981806T3 (enExample)
TW (1) TWI848981B (enExample)
WO (1) WO2020071407A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114685572A (zh) * 2022-06-02 2022-07-01 上海百力格生物技术有限公司 用于mgb核酸探针合成的氧化剂组合物及探针的合成方法
CN117143179A (zh) * 2023-09-18 2023-12-01 安徽峆一药业股份有限公司 一种凝血酶发色底物s-2238的合成方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024112876A2 (en) * 2022-11-23 2024-05-30 Prime Medicine, Inc. Split synthesis of long rnas

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CN102918156A (zh) * 2010-07-08 2013-02-06 株式会社博纳克 用于控制基因表达的单链核酸分子
CN103052711A (zh) * 2010-08-03 2013-04-17 株式会社博纳克 具有含氮脂环式骨架的单链核酸分子
JP2013153736A (ja) * 2012-01-07 2013-08-15 Bonac Corp ペプチド骨格を有する一本鎖核酸分子
CN104039961A (zh) * 2012-01-07 2014-09-10 株式会社博纳克 具有氨基酸骨架的单链核酸分子
WO2017035090A1 (en) * 2015-08-21 2017-03-02 Thomas Jefferson University Dumbbell-pcr: a method to quantify specific small rna variants with a single nucleotide resolution at terminal sequences
CN108350457A (zh) * 2015-10-30 2018-07-31 株式会社博纳克 稳定地含有抑制TGF-β1基因的表达的单链核酸分子的组合物

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WO1994007910A1 (en) 1992-09-28 1994-04-14 Research Corporation Technologies, Inc. New reagents for peptide couplings
DE60014542T2 (de) 1999-03-08 2006-03-09 Tokuyama Corp., Tokuyama Ein verfahren zur herstellung von carbonsäure-derivaten und ein kondensationshilfsmittel mit einem quarternären ammonium-salz
US6825347B2 (en) 2001-05-21 2004-11-30 Research Corporation Technologies, Inc. Uronium and immonium salts for peptide coupling
AU2003256857A1 (en) 2002-08-08 2004-02-25 Dharmacon, Inc. Short interfering rnas having a hairpin structure containing a non-nucleotide loop
JP5046002B2 (ja) 2007-03-02 2012-10-10 公益財団法人新産業創造研究機構 塩化トリアジン誘導体、その製造方法およびその使用
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JP6486700B2 (ja) 2015-01-29 2019-03-20 国立大学法人金沢大学 脱水縮合剤
JP6486699B2 (ja) 2015-01-29 2019-03-20 国立大学法人金沢大学 脱水縮合剤
JP2018187767A (ja) 2015-09-29 2018-11-29 綜研化学株式会社 撥水性部材及びその製造方法
JP6742606B2 (ja) 2016-02-25 2020-08-19 国立大学法人金沢大学 トリアジン含有ポリマー、その製造方法、及びその脱水縮合剤としての用途
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CN102918156A (zh) * 2010-07-08 2013-02-06 株式会社博纳克 用于控制基因表达的单链核酸分子
CN103052711A (zh) * 2010-08-03 2013-04-17 株式会社博纳克 具有含氮脂环式骨架的单链核酸分子
JP2013153736A (ja) * 2012-01-07 2013-08-15 Bonac Corp ペプチド骨格を有する一本鎖核酸分子
CN104039961A (zh) * 2012-01-07 2014-09-10 株式会社博纳克 具有氨基酸骨架的单链核酸分子
WO2017035090A1 (en) * 2015-08-21 2017-03-02 Thomas Jefferson University Dumbbell-pcr: a method to quantify specific small rna variants with a single nucleotide resolution at terminal sequences
CN108350457A (zh) * 2015-10-30 2018-07-31 株式会社博纳克 稳定地含有抑制TGF-β1基因的表达的单链核酸分子的组合物

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MATTHEW BADLAND ET AL.: "A comparative study of amide-bond forming reagents in aqueous media – Substrate scope and reagent compatibility", 《TETRAHEDRON LETTERS》, vol. 58, pages 4391, XP085273200, DOI: 10.1016/j.tetlet.2017.10.014 *
YU LIU ET AL.: "2’，2’-Ligation Demonstrates the Thermal Dependence of DNA-Directed Positional Control", 《TETRAHEDRON》, vol. 64, no. 36, pages 8417 - 8422, XP023315804, DOI: 10.1016/j.tet.2008.05.046 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114685572A (zh) * 2022-06-02 2022-07-01 上海百力格生物技术有限公司 用于mgb核酸探针合成的氧化剂组合物及探针的合成方法
CN117143179A (zh) * 2023-09-18 2023-12-01 安徽峆一药业股份有限公司 一种凝血酶发色底物s-2238的合成方法

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JPWO2020071407A1 (ja) 2021-09-02
EP3862431A1 (en) 2021-08-11
EP3862431B1 (en) 2024-06-26
US11891602B2 (en) 2024-02-06
TWI848981B (zh) 2024-07-21
US20240110186A1 (en) 2024-04-04
US20210317495A1 (en) 2021-10-14
JP7405086B2 (ja) 2023-12-26
KR20210069628A (ko) 2021-06-11
WO2020071407A1 (ja) 2020-04-09
TW202022117A (zh) 2020-06-16
EP3862431A4 (en) 2023-05-10
ES2981806T3 (es) 2024-10-10

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