KR20210069628A - 헤어핀형 단일쇄 rna 분자의 제조 방법 - Google Patents

헤어핀형 단일쇄 rna 분자의 제조 방법 Download PDF

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KR20210069628A
KR20210069628A KR1020217007240A KR20217007240A KR20210069628A KR 20210069628 A KR20210069628 A KR 20210069628A KR 1020217007240 A KR1020217007240 A KR 1020217007240A KR 20217007240 A KR20217007240 A KR 20217007240A KR 20210069628 A KR20210069628 A KR 20210069628A
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condensing agent
rna molecule
dehydration condensing
group
linker
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Korean (ko)
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쿄헤이 코시모토
카츠히코 이세키
히데아키 이나다
타츠야 후지타
케이이치 오키무라
무네타카 쿠니시마
타다아키 오기
에리코 아오키
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도레이 카부시키가이샤
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Publication of KR20210069628A publication Critical patent/KR20210069628A/ko
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1136—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against growth factors, growth regulators, cytokines, lymphokines or hormones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Endocrinology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
KR1020217007240A 2018-10-02 2019-10-02 헤어핀형 단일쇄 rna 분자의 제조 방법 Pending KR20210069628A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JPJP-P-2018-187767 2018-10-02
JP2018187767 2018-10-02
PCT/JP2019/038860 WO2020071407A1 (ja) 2018-10-02 2019-10-02 ヘアピン型一本鎖ｒｎａ分子の製造方法

Publications (1)

Publication Number Publication Date
KR20210069628A true KR20210069628A (ko) 2021-06-11

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KR1020217007240A Pending KR20210069628A (ko) 2018-10-02 2019-10-02 헤어핀형 단일쇄 rna 분자의 제조 방법

Country Status (8)

Country Link
US (2) US11891602B2 (enExample)
EP (1) EP3862431B1 (enExample)
JP (1) JP7405086B2 (enExample)
KR (1) KR20210069628A (enExample)
CN (1) CN112673102A (enExample)
ES (1) ES2981806T3 (enExample)
TW (1) TWI848981B (enExample)
WO (1) WO2020071407A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114685572B (zh) * 2022-06-02 2022-08-30 上海百力格生物技术有限公司 用于mgb核酸探针合成的氧化剂组合物及探针的合成方法
WO2024112876A2 (en) * 2022-11-23 2024-05-30 Prime Medicine, Inc. Split synthesis of long rnas
CN117143179B (zh) * 2023-09-18 2024-07-12 安徽峆一药业股份有限公司 一种凝血酶发色底物s-2238的合成方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040058886A1 (en) 2002-08-08 2004-03-25 Dharmacon, Inc. Short interfering RNAs having a hairpin structure containing a non-nucleotide loop
WO2012017919A1 (ja) 2010-08-03 2012-02-09 株式会社ボナック 含窒素脂環式骨格を有する一本鎖核酸分子

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WO1994007910A1 (en) 1992-09-28 1994-04-14 Research Corporation Technologies, Inc. New reagents for peptide couplings
DE60014542T2 (de) 1999-03-08 2006-03-09 Tokuyama Corp., Tokuyama Ein verfahren zur herstellung von carbonsäure-derivaten und ein kondensationshilfsmittel mit einem quarternären ammonium-salz
US6825347B2 (en) 2001-05-21 2004-11-30 Research Corporation Technologies, Inc. Uronium and immonium salts for peptide coupling
JP5046002B2 (ja) 2007-03-02 2012-10-10 公益財団法人新産業創造研究機構 塩化トリアジン誘導体、その製造方法およびその使用
SG186899A1 (en) 2010-07-08 2013-02-28 Bonac Corp Single-strand nucleic acid molecule for controlling gene expression
WO2013103146A1 (ja) 2012-01-07 2013-07-11 株式会社ボナック アミノ酸骨格を有する一本鎖核酸分子
JP2013153736A (ja) 2012-01-07 2013-08-15 Bonac Corp ペプチド骨格を有する一本鎖核酸分子
EP3254700B1 (en) * 2012-03-04 2019-10-23 Bonac Corporation Micro-rna inhibitor
WO2015093495A1 (ja) * 2013-12-16 2015-06-25 株式会社ボナック ＴＧＦ－β１遺伝子発現制御のための一本鎖核酸分子
JP6486700B2 (ja) 2015-01-29 2019-03-20 国立大学法人金沢大学 脱水縮合剤
JP6486699B2 (ja) 2015-01-29 2019-03-20 国立大学法人金沢大学 脱水縮合剤
WO2017035090A1 (en) 2015-08-21 2017-03-02 Thomas Jefferson University Dumbbell-pcr: a method to quantify specific small rna variants with a single nucleotide resolution at terminal sequences
JP2018187767A (ja) 2015-09-29 2018-11-29 綜研化学株式会社 撥水性部材及びその製造方法
EP3369816B1 (en) * 2015-10-30 2024-03-13 Toray Industries, Inc. Composition stably containing single-stranded nucleic acid molecule that suppresses expression of tgf-beta1 gene
JP6742606B2 (ja) 2016-02-25 2020-08-19 国立大学法人金沢大学 トリアジン含有ポリマー、その製造方法、及びその脱水縮合剤としての用途
US20210115485A1 (en) * 2018-03-30 2021-04-22 Sumitomo Chemical Company, Limited Method for producing single-strand rna
EP3778886A4 (en) * 2018-03-30 2022-11-02 Toray Industries, Inc. Method for producing hairpin single-stranded rna molecule

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Publication number Priority date Publication date Assignee Title
US20040058886A1 (en) 2002-08-08 2004-03-25 Dharmacon, Inc. Short interfering RNAs having a hairpin structure containing a non-nucleotide loop
WO2012017919A1 (ja) 2010-08-03 2012-02-09 株式会社ボナック 含窒素脂環式骨格を有する一本鎖核酸分子

Non-Patent Citations (4)

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Title
Badland 등, Tetrahedron Letters, 2017년, 제 58권, p.4391-4394.
Fire 등, Nature, 1998년, 제 391권, p.806-811
Guidebook for the Synthesis OF Oligonucleotides Product Guide 2015/16, Link Technologies Ltd., 2017년, 제 58권, p.49-51.
Liu 등, Tetrahedron, 2008년, 제 64권, p.8417-8422.

Also Published As

Publication number Publication date
JPWO2020071407A1 (ja) 2021-09-02
EP3862431A1 (en) 2021-08-11
EP3862431B1 (en) 2024-06-26
CN112673102A (zh) 2021-04-16
US11891602B2 (en) 2024-02-06
TWI848981B (zh) 2024-07-21
US20240110186A1 (en) 2024-04-04
US20210317495A1 (en) 2021-10-14
JP7405086B2 (ja) 2023-12-26
WO2020071407A1 (ja) 2020-04-09
TW202022117A (zh) 2020-06-16
EP3862431A4 (en) 2023-05-10
ES2981806T3 (es) 2024-10-10

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