TWI848981B - 新穎亞磷醯胺、及髮夾型單股ｒｎａ分子之製造方法 - Google Patents

新穎亞磷醯胺、及髮夾型單股ｒｎａ分子之製造方法 Download PDF

Info

Publication number
TWI848981B
TWI848981B TW108135638A TW108135638A TWI848981B TW I848981 B TWI848981 B TW I848981B TW 108135638 A TW108135638 A TW 108135638A TW 108135638 A TW108135638 A TW 108135638A TW I848981 B TWI848981 B TW I848981B
Authority
TW
Taiwan
Prior art keywords
rna molecule
represented
group
following formula
condensation agent
Prior art date
Application number
TW108135638A
Other languages
English (en)
Chinese (zh)
Other versions
TW202022117A (zh
Inventor
越本恭平
伊關克彥
稻田英朗
藤田達也
沖村慶一
國嶋崇隆
大木忠明
青木繪里子
Original Assignee
日商東麗股份有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 日商東麗股份有限公司 filed Critical 日商東麗股份有限公司
Publication of TW202022117A publication Critical patent/TW202022117A/zh
Application granted granted Critical
Publication of TWI848981B publication Critical patent/TWI848981B/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1136—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against growth factors, growth regulators, cytokines, lymphokines or hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Endocrinology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
TW108135638A 2018-10-02 2019-10-02 新穎亞磷醯胺、及髮夾型單股ｒｎａ分子之製造方法 TWI848981B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2018187767 2018-10-02
JP2018-187767 2018-10-02

Publications (2)

Publication Number Publication Date
TW202022117A TW202022117A (zh) 2020-06-16
TWI848981B true TWI848981B (zh) 2024-07-21

Family

ID=70055619

Family Applications (1)

Application Number Title Priority Date Filing Date
TW108135638A TWI848981B (zh) 2018-10-02 2019-10-02 新穎亞磷醯胺、及髮夾型單股ｒｎａ分子之製造方法

Country Status (8)

Country Link
US (2) US11891602B2 (enExample)
EP (1) EP3862431B1 (enExample)
JP (1) JP7405086B2 (enExample)
KR (1) KR20210069628A (enExample)
CN (1) CN112673102A (enExample)
ES (1) ES2981806T3 (enExample)
TW (1) TWI848981B (enExample)
WO (1) WO2020071407A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114685572B (zh) * 2022-06-02 2022-08-30 上海百力格生物技术有限公司 用于mgb核酸探针合成的氧化剂组合物及探针的合成方法
WO2024112876A2 (en) * 2022-11-23 2024-05-30 Prime Medicine, Inc. Split synthesis of long rnas
CN117143179B (zh) * 2023-09-18 2024-07-12 安徽峆一药业股份有限公司 一种凝血酶发色底物s-2238的合成方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW201219570A (en) * 2010-08-03 2012-05-16 Bonac Corp Single-stranded nucleic acid molecule having nitrogen-containing alicyclic skeleton

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994007910A1 (en) 1992-09-28 1994-04-14 Research Corporation Technologies, Inc. New reagents for peptide couplings
DE60014542T2 (de) 1999-03-08 2006-03-09 Tokuyama Corp., Tokuyama Ein verfahren zur herstellung von carbonsäure-derivaten und ein kondensationshilfsmittel mit einem quarternären ammonium-salz
US6825347B2 (en) 2001-05-21 2004-11-30 Research Corporation Technologies, Inc. Uronium and immonium salts for peptide coupling
AU2003256857A1 (en) 2002-08-08 2004-02-25 Dharmacon, Inc. Short interfering rnas having a hairpin structure containing a non-nucleotide loop
JP5046002B2 (ja) 2007-03-02 2012-10-10 公益財団法人新産業創造研究機構 塩化トリアジン誘導体、その製造方法およびその使用
SG186899A1 (en) 2010-07-08 2013-02-28 Bonac Corp Single-strand nucleic acid molecule for controlling gene expression
WO2013103146A1 (ja) 2012-01-07 2013-07-11 株式会社ボナック アミノ酸骨格を有する一本鎖核酸分子
JP2013153736A (ja) 2012-01-07 2013-08-15 Bonac Corp ペプチド骨格を有する一本鎖核酸分子
EP3254700B1 (en) * 2012-03-04 2019-10-23 Bonac Corporation Micro-rna inhibitor
WO2015093495A1 (ja) * 2013-12-16 2015-06-25 株式会社ボナック ＴＧＦ－β１遺伝子発現制御のための一本鎖核酸分子
JP6486700B2 (ja) 2015-01-29 2019-03-20 国立大学法人金沢大学 脱水縮合剤
JP6486699B2 (ja) 2015-01-29 2019-03-20 国立大学法人金沢大学 脱水縮合剤
WO2017035090A1 (en) 2015-08-21 2017-03-02 Thomas Jefferson University Dumbbell-pcr: a method to quantify specific small rna variants with a single nucleotide resolution at terminal sequences
JP2018187767A (ja) 2015-09-29 2018-11-29 綜研化学株式会社 撥水性部材及びその製造方法
EP3369816B1 (en) * 2015-10-30 2024-03-13 Toray Industries, Inc. Composition stably containing single-stranded nucleic acid molecule that suppresses expression of tgf-beta1 gene
JP6742606B2 (ja) 2016-02-25 2020-08-19 国立大学法人金沢大学 トリアジン含有ポリマー、その製造方法、及びその脱水縮合剤としての用途
US20210115485A1 (en) * 2018-03-30 2021-04-22 Sumitomo Chemical Company, Limited Method for producing single-strand rna
EP3778886A4 (en) * 2018-03-30 2022-11-02 Toray Industries, Inc. Method for producing hairpin single-stranded rna molecule

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW201219570A (en) * 2010-08-03 2012-05-16 Bonac Corp Single-stranded nucleic acid molecule having nitrogen-containing alicyclic skeleton
TW201615835A (zh) * 2010-08-03 2016-05-01 伯納克股份有限公司 具有含氮脂環骨架之單股核酸分子（二）

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
期刊 Liu et al., "2′,2′-Ligation Demonstrates the Thermal Dependence of DNA-Directed Positional Control" Tetrahedron, 64(36), Elsevier, 2008 Jan, p. 8417–8422. *

Also Published As

Publication number Publication date
JPWO2020071407A1 (ja) 2021-09-02
EP3862431A1 (en) 2021-08-11
EP3862431B1 (en) 2024-06-26
CN112673102A (zh) 2021-04-16
US11891602B2 (en) 2024-02-06
US20240110186A1 (en) 2024-04-04
US20210317495A1 (en) 2021-10-14
JP7405086B2 (ja) 2023-12-26
KR20210069628A (ko) 2021-06-11
WO2020071407A1 (ja) 2020-04-09
TW202022117A (zh) 2020-06-16
EP3862431A4 (en) 2023-05-10
ES2981806T3 (es) 2024-10-10

Similar Documents

Publication Publication Date Title
US20240110186A1 (en) Method of producing hairpin single-stranded rna molecules
ES2745504T3 (es) Acidos nucleicos peptídicos gamma que contienen miniPEG preorganizados conformacionalmente
US8912318B2 (en) Nucleobase-functionalized conformationally restricted nucleotides and oligonucleotides for targeting nucleic acids
PL184920B1 (pl) Nowe analogi oligonukleotydówĆ sposób ich wytwarzania i środek farmaceutyczny
TWI772632B (zh) 髮夾型單股ｒｎａ分子之製造方法、單股寡ｒｎａ分子及包含其之套組
IL301185A (en) Lipid conjugates for the delivery of therapeutic agents
Zhou et al. 2-(4-Tolylsulfonyl) ethoxymethyl (TEM)—a new 2′-OH protecting group for solid-supported RNA synthesis
TW202613310A (zh) 含有三氮唑結構的GalNAc化合物及其寡核苷酸綴合物
CN116514795A (zh) 3CLpro蛋白酶抑制剂的制备方法
JP7313619B2 (ja) 化合物、蛍光標識導入剤、及び蛍光標識導入方法
JP6429264B2 (ja) ボラノホスフェート化合物、及び核酸オリゴマー
US8865898B2 (en) Nucleoside analog or salt thereof, oligonucleotide analog, gene expression inhibitor, and nucleic-acid probe for detecting gene
US11938187B2 (en) Methods and compositions for synthesis of two-photon cleavable phosphoramidite molecules for oligonucleotide conjugation
ES2403129B1 (es) Compuestos maleimido-furanilo útiles en un procedimiento general de preparación de derivados maleimido-oligonucleótido
Efthymiou et al. Synthesis and properties of oligonucleotides that contain a triazole‐linked nucleic acid dimer
KR102934598B1 (ko) 신규한 몰포리노 올리고뉴클레오티드 유도체
JP5251125B2 (ja) ユニバーサル塩基含有ポリマー
JP4805143B2 (ja) ２‘水酸基を修飾された新規人工ｒｎａ
JPWO2023127918A5 (enExample)
JP2021178790A (ja) 核酸の製造方法、およびシランカップリング処理用組成物
JPWO2007029364A1 (ja) ピロールアミド４量体を含む２’−デオキシグアノシンハイブリッド化合物及びその製造方法、そのための製造中間体
JPH0753587A (ja) オリゴヌクレオチドの合成法
CA3108806A1 (en) Reagents based on a tertiary amine backbone to introduce chemical functionality in nucleic acids and sequence-controlled polymers

Legal Events

Date Code Title Description
MM4A Annulment or lapse of patent due to non-payment of fees