JP7316275B2 - ｓｓＤＮＡの切断及び標的ＤＮＡの検出ためのＶ型ＣＲＩＳＰＲ／Ｃａｓエフェクタータンパク質 - Google Patents

ｓｓＤＮＡの切断及び標的ＤＮＡの検出ためのＶ型ＣＲＩＳＰＲ／Ｃａｓエフェクタータンパク質 Download PDF

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JP7316275B2
JP7316275B2 JP2020528333A JP2020528333A JP7316275B2 JP 7316275 B2 JP7316275 B2 JP 7316275B2 JP 2020528333 A JP2020528333 A JP 2020528333A JP 2020528333 A JP2020528333 A JP 2020528333A JP 7316275 B2 JP7316275 B2 JP 7316275B2
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crispr
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ジェニファー エー． ダウドナ
ジャニス エス． チェン
ルーカス ベンジャミン ハリントン
エンボ マー
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University of California San Diego UCSD
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JP2020528333A 2017-11-22 2018-11-20 ｓｓＤＮＡの切断及び標的ＤＮＡの検出ためのＶ型ＣＲＩＳＰＲ／Ｃａｓエフェクタータンパク質 Active JP7316275B2 (ja)

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Application Number Priority Date Filing Date Title
US201762590106P 2017-11-22 2017-11-22
US62/590,106 2017-11-22
US201862626593P 2018-02-05 2018-02-05
US62/626,593 2018-02-05
US15/897,089 US10253365B1 (en) 2017-11-22 2018-02-14 Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs
US15/897,089 2018-02-14
PCT/US2018/062052 WO2019104058A1 (en) 2017-11-22 2018-11-20 Type v crispr/cas effector proteins for cleaving ssdnas and detecting target dnas

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JP2021508238A JP2021508238A (ja) 2021-03-04
JP2021508238A5 JP2021508238A5 (https=) 2022-01-04
JP7316275B2 true JP7316275B2 (ja) 2023-07-27

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US (6) US10253365B1 (https=)
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CN (1) CN111630163A (https=)
AU (1) AU2018373026B2 (https=)
CA (1) CA3081937A1 (https=)
IL (1) IL274275A (https=)
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Families Citing this family (167)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6847660B2 (ja) 2013-06-07 2021-03-24 マサチューセッツ インスティテュート オブ テクノロジー リガンドをコードする合成バイオマーカーのアフィニティベースの検出
GB201506509D0 (en) 2015-04-16 2015-06-03 Univ Wageningen Nuclease-mediated genome editing
US10648020B2 (en) 2015-06-18 2020-05-12 The Broad Institute, Inc. CRISPR enzymes and systems
WO2017106657A1 (en) 2015-12-18 2017-06-22 The Broad Institute Inc. Novel crispr enzymes and systems
EP3445853A1 (en) 2016-04-19 2019-02-27 The Broad Institute, Inc. Cpf1 complexes with reduced indel activity
CN110382692A (zh) 2016-04-19 2019-10-25 博德研究所 新型crispr酶以及系统
US10337051B2 (en) 2016-06-16 2019-07-02 The Regents Of The University Of California Methods and compositions for detecting a target RNA
JP7306696B2 (ja) 2016-09-30 2023-07-11 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア Ｒｎａ誘導型核酸修飾酵素及びその使用方法
CA3038960A1 (en) 2016-09-30 2018-04-05 The Regents Of The University Of California Rna-guided nucleic acid modifying enzymes and methods of use thereof
CA3059358A1 (en) 2017-04-07 2018-10-11 Massachusetts Institute Of Technology Methods to spatially profile protease activity in tissue and sections
US11584955B2 (en) 2017-07-14 2023-02-21 Shanghai Tolo Biotechnology Company Limited Application of Cas protein, method for detecting target nucleic acid molecule and kit
CN107488710B (zh) 2017-07-14 2020-09-22 上海吐露港生物科技有限公司 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒
WO2019089808A1 (en) 2017-11-01 2019-05-09 The Regents Of The University Of California Class 2 crispr/cas compositions and methods of use
JP2021503278A (ja) 2017-11-01 2021-02-12 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア ＣａｓＺ組成物及び使用方法
WO2019089804A1 (en) 2017-11-01 2019-05-09 The Regents Of The University Of California Casy compositions and methods of use
US10253365B1 (en) 2017-11-22 2019-04-09 The Regents Of The University Of California Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs
CN110551800B (zh) * 2018-06-03 2022-08-19 上海吐露港生物科技有限公司 一种耐高温Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒
EP3830301B1 (en) 2018-08-01 2024-05-22 Mammoth Biosciences, Inc. Programmable nuclease compositions and methods of use thereof
EP3844303A4 (en) * 2018-08-27 2022-06-01 The Regents of The University of California Reporter nucleic acids for type v crispr-mediated detection
US12540352B2 (en) * 2018-09-20 2026-02-03 Institute Of Zoology, Chinese Academy Of Sciences Method for detecting nucleic acid
EP3856921B1 (en) 2018-09-25 2026-04-29 Massachusetts Institute Of Technology Lung protease nanosensors and uses thereof
US11407995B1 (en) 2018-10-26 2022-08-09 Inari Agriculture Technology, Inc. RNA-guided nucleases and DNA binding proteins
US11434477B1 (en) 2018-11-02 2022-09-06 Inari Agriculture Technology, Inc. RNA-guided nucleases and DNA binding proteins
CA3117730A1 (en) 2018-11-09 2020-05-14 Inari Agriculture, Inc. Rna-guided nucleases and dna binding proteins
EP3884064A4 (en) * 2018-11-19 2022-08-10 The Regents of The University of California METHODS FOR DETECTING AND SEQUENCING A TARGET NUCLEIC ACID
EP3931313A2 (en) 2019-01-04 2022-01-05 Mammoth Biosciences, Inc. Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection
WO2020150560A1 (en) 2019-01-17 2020-07-23 Massachusetts Institute Of Technology Sensors for detecting and imaging of cancer metastasis
US10913941B2 (en) * 2019-02-14 2021-02-09 Metagenomi Ip Technologies, Llc Enzymes with RuvC domains
CA3130789A1 (en) 2019-03-07 2020-09-10 The Regents Of The University Of California Crispr-cas effector polypeptides and methods of use thereof
CN111812066B (zh) * 2019-04-10 2023-04-28 华东理工大学 基于CRISPR/Cas12a系统的生物传感器、试剂盒及其在小分子检测中的用途
CN110117818A (zh) * 2019-04-16 2019-08-13 天津大学 检测单基因突变的crispr高通量生物芯片
CN110129463B (zh) * 2019-05-06 2020-08-28 浙江大学 一种用于头状葡萄球菌检测的试剂盒
CN110117621B (zh) * 2019-05-24 2021-07-16 青岛农业大学 一种碱基编辑器及其制备方法和应用
PH12021553076A1 (en) 2019-06-07 2023-08-14 Scribe Therapeutics Inc Engineered casx systems
KR102905491B1 (ko) * 2019-06-25 2025-12-31 파라그라프 유에스에이 인코포레이티드 리포터 모이어티의 효소적 절단에 기반한 표적의 전자 검출
CN114072520A (zh) 2019-07-04 2022-02-18 国立研究开发法人理化学研究所 检测靶核酸片段的方法及试剂盒
US20220364159A1 (en) * 2019-07-26 2022-11-17 Mammoth Biosciences, Inc. Compositions for detection of dna and methods of use thereof
US12371695B2 (en) 2019-08-02 2025-07-29 Monsanto Technology Llc Methods and compositions to promote targeted genome modifications using HUH endonucleases
CN110541022B (zh) * 2019-08-09 2022-11-04 福建医科大学孟超肝胆医院(福州市传染病医院) 基于CRISPR-Cas12a系统的结核分枝杆菌复合群检测试剂盒
CN110819652A (zh) * 2019-08-21 2020-02-21 山东金城生物药业有限公司 一种谷氨酸棒杆菌同时基因敲除及基因表达抑制的双功能系统及应用
WO2021041846A1 (en) 2019-08-30 2021-03-04 Inari Agriculture, Inc. Rna-guided nucleases and dna binding proteins
CA3153301A1 (en) * 2019-09-05 2021-03-11 Benson Hill, Inc. Compositions and methods for modifying genomes
WO2021050755A1 (en) 2019-09-10 2021-03-18 Caspr Biotech Corporation Novel class 2 type ii and type v crispr-cas rna-guided endonucleases
EP4034152A1 (en) * 2019-09-25 2022-08-03 University College Dublin Nanoparticle compositions for gene therapy
CN110551812B (zh) * 2019-09-26 2020-08-18 暨南大学 一种诊断脊髓性肌萎缩症的CRISPR-Cas系统及其应用
WO2021072076A1 (en) * 2019-10-08 2021-04-15 Massachusetts Institute Of Technology Daughterless male mammals for non-human population suppression
GB201914568D0 (en) * 2019-10-09 2019-11-20 Governing Council Of The Univ Of Toronto A molecular sensing platform and methods of use
CN112708660B (zh) * 2019-10-24 2024-05-03 广州微远医疗器械有限公司 Crispr-poct检测组合物及其应用
US20220380835A1 (en) * 2019-11-08 2022-12-01 University Of Florida Research Foundation, Inc. Crispr complex-based detection system and method
CN111394337A (zh) * 2019-11-15 2020-07-10 武汉大学 II类V型CRISPR蛋白Lb2Cas12a及其在基因编辑的应用
WO2021108324A1 (en) * 2019-11-27 2021-06-03 Technical University Of Denmark Constructs, compositions and methods thereof having improved genome editing efficiency and specificity
CN112981004A (zh) * 2019-12-17 2021-06-18 广东菲鹏生物有限公司 一种检测hpv的试剂及其试剂盒和应用
EP4081533A4 (en) * 2019-12-23 2024-03-20 The Regents of The University of California CRISPR-CAS EFFECTOR POLYPEPTIDES AND METHOD OF USE THEREOF
NL2024572B1 (en) 2019-12-24 2021-09-06 Univ Delft Tech Secure communication using crispr-cas
KR102422254B1 (ko) * 2020-01-02 2022-07-19 서강대학교산학협력단 CRISPR/Cas12a를 이용한 표면증강 라만산란 기반 표적 DNA 검출용 센서
WO2021142109A1 (en) * 2020-01-07 2021-07-15 The Trustees Of Columbia University In The City Of New York Transposition-based diagnostics methods and devices
JP6940086B1 (ja) * 2020-01-24 2021-09-22 Ｃ４Ｕ株式会社 試料中の特定のｄｎａを検出する方法
IL295011A (en) 2020-01-27 2022-09-01 Sherlock Biosciences Inc Improved detection tests
CN115515626A (zh) * 2020-02-06 2022-12-23 猛犸生物科学公司 用于检测冠状病毒的组合物和方法
BR112022015365A2 (pt) * 2020-02-06 2022-09-20 The Administrators Of The Tulane Educational Fund Ensaio baseado em crispr para detecção de tb em fluidos corporais
CN111187856B (zh) * 2020-02-13 2020-11-13 上海科技大学 一种用于新冠病毒核酸快速检测的Cpf1试剂盒及其制备方法与应用
US20210262025A1 (en) * 2020-02-18 2021-08-26 Massachusetts Institute Of Technology Multiplexed in vivo disease sensing with nucleic acid-barcoded reporters
EP3875602A1 (en) 2020-03-05 2021-09-08 Nederlandse Organisatie voor toegepast- natuurwetenschappelijk Onderzoek TNO Nucleic acid fluorescence detection
GB2617658B (en) 2020-03-06 2024-04-17 Metagenomi Inc Class II, type V CRISPR systems
WO2021188669A1 (en) * 2020-03-18 2021-09-23 University Of Connecticut Crispr-cas12a reaction for rapid and highly sensitive isothermal nucleic acid detection
US11639523B2 (en) 2020-03-23 2023-05-02 The Broad Institute, Inc. Type V CRISPR-Cas systems and use thereof
CN111394430B (zh) * 2020-03-30 2021-05-25 重庆大学 一种基于CRISPR-Cas12a耦合增强型链置换扩增的检测系统及其应用
CN111257297B (zh) * 2020-03-31 2023-06-16 海南大学 一种基于Cas14a酶的RNA检测方法
KR20220167379A (ko) * 2020-04-10 2022-12-20 매머드 바이오사이언시즈 인크. 핵산 검출을 위한 하이-플렉스 가이드 풀링
WO2021216573A1 (en) * 2020-04-20 2021-10-28 The Regents Of The University Of California Scalable, easy-to-deploy system and reagents thereof for crispr-associated nuclease-based detection of pathogen genetic material
US20230203567A1 (en) 2020-04-22 2023-06-29 President And Fellows Of Harvard College Isothermal methods, compositions, kits, and systems for detecting nucleic acids
WO2021216868A1 (en) * 2020-04-22 2021-10-28 The Regents Of The University Of California Methods for detecting and sequencing a target nucleic acid
CN111690717B (zh) * 2020-04-30 2023-05-30 山东舜丰生物科技有限公司 基于crispr技术进行目标核酸检测的方法和系统
WO2021240443A1 (en) * 2020-05-27 2021-12-02 King Abdullah University Of Science And Technology Iscan: an rt-lamp-coupled crispr-cas module for rapid, sensitive detection of sars-cov-2
US20230193368A1 (en) * 2020-05-29 2023-06-22 University Of Florida Research Foundation Crispr/cas chain reaction systems and methods for amplifying the detection sensitivity of crispr-based target detection
CN111996236B (zh) * 2020-05-29 2021-06-29 山东舜丰生物科技有限公司 基于crispr技术进行靶核酸检测的方法
CN111560482B (zh) * 2020-06-11 2024-02-23 亚能生物技术（深圳）有限公司 基于CRISPR/Cas和核酸试纸的检测方法和人乳头瘤病毒检测试剂盒
CN111593145B (zh) * 2020-06-11 2023-05-30 亚能生物技术（深圳）有限公司 一种CRISPR/Cas12一步核酸检测方法及新型冠状病毒检测试剂盒
WO2021254267A1 (zh) * 2020-06-16 2021-12-23 山东舜丰生物科技有限公司 利用核酸类似物或碱基修饰物进行靶核酸检测的方法
CN111690720B (zh) * 2020-06-16 2021-06-15 山东舜丰生物科技有限公司 利用修饰的单链核酸进行靶核酸检测的方法
CN111876469B (zh) * 2020-06-18 2023-11-24 山东舜丰生物科技有限公司 利用核酸类似物进行靶核酸检测的方法
CN111690773B (zh) * 2020-06-17 2021-08-20 山东舜丰生物科技有限公司 利用新型Cas酶进行目标核酸检测的方法和系统
WO2021262099A1 (en) * 2020-06-26 2021-12-30 Nanyang Technological University Crispr-based methods for the detection of nucleic acids in a sample
WO2022006536A1 (en) * 2020-07-03 2022-01-06 The Regents Of The University Of California Crispr-cas-based detection of sars-cov-2 using recombinase polymerase amplification
CN112795624B (zh) * 2020-07-17 2021-11-19 山东舜丰生物科技有限公司 利用含有无碱基间隔物的核酸检测器检测靶核酸的方法
EP4182456A4 (en) 2020-07-17 2024-08-21 Trustees of Boston University Viral detection systems and uses thereof
WO2022020431A2 (en) 2020-07-21 2022-01-27 Trustees Of Boston University Inducible control of gene expression
US20240035968A1 (en) * 2020-08-27 2024-02-01 Mbt Co., Ltd. Method for detecting target biomolecule in biological sample, disposable optical fiber sensor therefor, and cartridge thereof
KR102457309B1 (ko) * 2020-08-27 2022-10-24 (주)엠비티 크리스퍼-카스 시스템 및 fo-lspr 센서를 이용하여 생물학적 시료 내 표적 핵산을 검출하는 방법 및 이를 구현하기 위한 장치
CN113801917B (zh) * 2020-08-28 2022-11-22 山东舜丰生物科技有限公司 基于crispr技术进行多重核酸检测的方法
WO2022061166A1 (en) * 2020-09-17 2022-03-24 Mammoth Biosciences, Inc. Compositions and methods for detection of a nucleic acid
CN113293198B (zh) * 2020-09-18 2022-12-02 山东舜丰生物科技有限公司 基于crispr技术进行靶核酸多重检测的方法
CN112111605A (zh) * 2020-09-21 2020-12-22 中山大学附属第一医院 基于CRISPR-Cas12a和G四链体-血红素的HPV的检测试剂盒
CN115786285B (zh) 2020-09-23 2025-06-24 山东舜丰生物科技有限公司 抗除草剂乙酰辅酶a羧化酶突变体及其应用
KR102493512B1 (ko) * 2020-10-08 2023-02-01 주식회사 진코어 CRISPR/Cas12a 시스템을 위한 엔지니어링 된 crRNA
CN116391023A (zh) * 2020-10-13 2023-07-04 佳能株式会社 核酸检测装置及检测核酸的方法
KR102738301B1 (ko) * 2020-11-02 2024-12-04 주식회사 이지다이아텍 다중 진단을 위한 핵산 분리 및 검출용 미세입자 프로브
US20230072431A1 (en) * 2020-11-03 2023-03-09 Science Solutions Llc Novel class 2 crispr-cas rna-guided endonucleases
WO2022098681A2 (en) 2020-11-03 2022-05-12 Caspr Biotech Corporation Novel class 2 crispr-cas rna-guided endonucleases
CN114507716B (zh) * 2020-11-16 2024-07-12 北京迅识科技有限公司 检测样品中靶核酸的方法
WO2022119919A1 (en) * 2020-12-01 2022-06-09 Howell Alexandra Compositions and methods for cleaving viral genomes
AU2021392719B2 (en) 2020-12-03 2025-08-14 Scribe Therapeutics Inc. Engineered class 2 type v crispr systems
JP7850156B2 (ja) * 2020-12-17 2026-04-22 モンサント テクノロジー エルエルシー 操作されたｓｓＤＮａｓｅ不含ＣＲＩＳＰＲエンドヌクレアーゼ
CN112626179B (zh) * 2020-12-21 2023-05-30 重庆医科大学 一种CTSDR与CRISPR/Cas联用检测miRNA的荧光传感器及其制备与应用
US20240094199A1 (en) * 2020-12-28 2024-03-21 Riken Method and kit for detecting target nucleic acid fragment
CN112905958B (zh) * 2021-01-27 2024-04-19 南京国电南自电网自动化有限公司 基于测控装置的短时数据窗遥测数据状态辨识方法及系统
EP4291643A4 (en) * 2021-02-09 2025-06-04 Mammoth Biosciences, Inc. PROGRAMMABLE NUCLEASES AND METHODS OF USE
WO2022204427A1 (en) * 2021-03-24 2022-09-29 Northwestern University Crispr-mediated cleavage of oligonucleotide-detectable marker conjugates for detection of target analytes
CN112980929B (zh) * 2021-04-13 2021-07-20 广州海思医疗科技有限公司 一种快速检测hla-b*1502的试剂盒
EP4323544A1 (en) 2021-04-15 2024-02-21 Amazon Technologies, Inc. Nucleases for signal amplification
KR102512999B1 (ko) * 2021-04-23 2023-03-23 솔바이오 주식회사 금 나노 입자-DNA 복합체 및 CRISPR/Cas12a 복합체를 포함하는 바이오센서, 및 이를 이용한 표적 DNA 검출 방법
EP4337701A4 (en) * 2021-05-10 2025-04-09 Mammoth Biosciences, Inc. EFFECTOR PROTEINS AND METHODS OF USE
WO2022247873A1 (zh) 2021-05-27 2022-12-01 中国科学院动物研究所 工程化的Cas12i核酸酶、效应蛋白及其用途
US20220389418A1 (en) 2021-06-01 2022-12-08 Helmholtz-Institut Für Rna-Basierte Nfektionsforschung (Hzi) Rna-guided cas nucleases and uses thereof in diagnostics and therapy
IL308806A (en) 2021-06-01 2024-01-01 Arbor Biotechnologies Inc Gene editing systems including nuclease crisper and their uses
CN113308518B (zh) * 2021-06-02 2022-10-11 重庆大学 Dna甲基化的超敏检测方法及其应用
US20240287592A1 (en) * 2021-06-10 2024-08-29 New England Biolabs, Inc. An Isothermal Diagnostic Test that Utilizes a Cas Protein and a Polymerase
CN113403412A (zh) * 2021-06-24 2021-09-17 中国农业科学院兰州兽医研究所 一种基于等温扩增-CRISPR/Cas12a技术检测弓形虫的试剂盒及其应用
CN113980957A (zh) * 2021-07-02 2022-01-28 苏州系统医学研究所 基于CRISPR/Cas12a的单链DNA探针及检测目标核酸的方法
JP2024524773A (ja) 2021-07-08 2024-07-05 モンタナ ステート ユニバーシティ Ｃｒｉｓｐｒを用いたプログラム可能なｒｎａ編集
US20230167487A2 (en) 2021-07-12 2023-06-01 Labsimply, Inc. Crispr cascade assay
CN113699148B (zh) * 2021-07-15 2024-01-09 四川大学 一种超灵敏抗体检测方法
CN113552103B (zh) * 2021-07-20 2022-12-30 济南大学 一种基于CRISPR-Cas系统的检测外泌体的荧光生物传感器
US11814689B2 (en) 2021-07-21 2023-11-14 Montana State University Nucleic acid detection using type III CRISPR complex
CN113552106B (zh) * 2021-07-23 2022-12-30 济南大学 一种检测ATP、谷胱甘肽、Fpg糖基化酶的通用荧光传感器
CN113667718B (zh) * 2021-08-25 2023-11-28 山东舜丰生物科技有限公司 利用双链核酸检测器进行靶核酸检测的方法
CA3225082A1 (en) 2021-08-27 2023-03-02 Brian C. Thomas Enzymes with ruvc domains
EP4399290A4 (en) * 2021-09-08 2025-10-01 Metagenomi Inc CLASS II TYPE V CRISPR SYSTEMS
CN113684317B (zh) * 2021-09-09 2023-09-26 贵州中医药大学第二附属医院 一种基于CRISPR-Cas12b对乙肝病毒B型和C型的超灵敏快速检测及鉴别系统
US20230107635A1 (en) * 2021-10-01 2023-04-06 Oncogenesis, Inc. Crispr assay for rapid, enhanced screening of hpv-related disease
MX2024006257A (es) 2021-11-24 2024-07-29 Metagenomi Inc Sistemas de endonucleasas.
CN116200368A (zh) * 2021-11-30 2023-06-02 上海科技大学 一种基于c2c9核酸酶的新型基因组编辑系统及其应用
CN114381550B (zh) * 2021-12-03 2023-04-28 中国科学院精密测量科学与技术创新研究院 用于hpv分型的多靶标核酸检测试剂盒和检测方法
US11820983B2 (en) 2021-12-13 2023-11-21 Labsimply, Inc. Tuning cascade assay kinetics via molecular design
US20230279375A1 (en) 2021-12-13 2023-09-07 Labsimply, Inc. Signal boost cascade assay
WO2023122663A2 (en) * 2021-12-23 2023-06-29 Mammoth Biosciences, Inc. Effector proteins and methods of use
CN114507712B (zh) * 2021-12-23 2024-04-19 南京师范大学 一种基于CRISPR/Cas12的肝素检测方法及其检测试剂盒
US20250163498A1 (en) * 2022-01-04 2025-05-22 Board Of Regents, The University Of Texas System Methods and compositions related to nucleic acid-based fluorescent nanocluster probes
EP4495257A1 (en) * 2022-04-21 2025-01-22 Ezdiatech Inc. Microparticle probe for single nucleotide polymorphism analysis
US20230357869A1 (en) * 2022-05-03 2023-11-09 The Penn State Research Foundation Methods and compositions for supersensitive and specific detection of citrus greening and phytoplasma pathogens
CN119698489A (zh) * 2022-06-16 2025-03-25 易兹代亚科技股份有限公司 包含磁性粒子和失活基因剪刀的诊断微粒探针、以及包含其的多重诊断系统与多重诊断方法
CN115976164B (zh) * 2022-09-07 2025-10-14 北京迅识科技有限公司 用于crispr级联核酸检测系统的核酸分子及应用
US12091689B2 (en) 2022-09-30 2024-09-17 Vedabio, Inc. Delivery of therapeutics in vivo via a CRISPR-based cascade system
US11982677B2 (en) 2022-10-02 2024-05-14 Vedabio, Inc. Dimerization screening assays
WO2024081156A1 (en) 2022-10-14 2024-04-18 Vedabio, Inc. Detection of nucleic acid and non-nucleic acid target molecules
WO2024091907A1 (en) * 2022-10-24 2024-05-02 Mammoth Biosciences, Inc. Compositions and methods for modifying the hpv16 genome
CN116121344B (zh) * 2022-11-08 2024-09-13 重庆医科大学 CtSDR反应系统、基于CRISPR的RNA原位成像方法、研究方法及应用
CN115820818B (zh) * 2022-12-13 2024-02-23 博迪泰（厦门）生物科技有限公司 一种一步法核酸检测方法及其应用
WO2024129512A2 (en) * 2022-12-16 2024-06-20 Monsanto Technology Llc Compositions and methods for site-directed integration
CN115820939B (zh) * 2022-12-18 2024-09-13 厦门大学 用于猴痘病毒检测的crRNA、核酸分子组合物、检测体系及应用
US12435357B2 (en) 2022-12-22 2025-10-07 Kanso Diagnostics Ltd. Diagnostic device for detecting a target nucleic acid molecule in a biological sample
CN118275664A (zh) 2022-12-30 2024-07-02 上海吐露港生物科技有限公司 基于拆分Cas蛋白的非核酸靶标检测方法及应用
WO2024253707A2 (en) 2023-01-07 2024-12-12 Vedabio, Inc. Engineered nucleic acid-guided nucleases
WO2024151493A1 (en) 2023-01-10 2024-07-18 Vedabio, Inc. Sample splitting for multiplexed detection of nucleic acids without amplification
WO2024173645A1 (en) 2023-02-15 2024-08-22 Arbor Biotechnologies, Inc. Gene editing method for inhibiting aberrant splicing in stathmin 2 (stmn2) transcript
WO2024206676A1 (en) * 2023-03-29 2024-10-03 Scribe Therapeutics Inc. Compositions and methods for the targeting of lpa
CN116819073B (zh) * 2023-04-27 2025-10-31 中国干细胞集团上海生物科技有限公司 检测造血干细胞的组合物及其方法
KR102674780B1 (ko) * 2023-05-30 2024-06-14 한국생명공학연구원 병원체 현장 검출을 위한 이중효소 증폭 기반 비색센서 시스템
CN116590444A (zh) * 2023-06-09 2023-08-15 四川大学 一种单碱基突变耐药菌的可视化检测技术
EP4530357A1 (en) * 2023-09-27 2025-04-02 Helmholtz-Zentrum für Infektionsforschung GmbH Enhanced nucleic-acid detection using truncated target nucleic acids
US20250223659A1 (en) 2024-01-08 2025-07-10 Roche Molecular Systems, Inc. Methods and uses of crispr cascade reactions for crispr diagnostics
WO2025171210A1 (en) 2024-02-09 2025-08-14 Arbor Biotechnologies, Inc. Compositions and methods for gene editing via homology-mediated end joining
CN118222765A (zh) * 2024-05-23 2024-06-21 江西乐成欣生生物技术研究有限责任公司 一种基于CRISPR的HBVpgRNA检测试剂盒
WO2026015829A2 (en) 2024-07-12 2026-01-15 Arbor Biotechnologies, Inc. Small reverse transcriptases and gene editing systems comprising such
WO2026015832A2 (en) 2024-07-12 2026-01-15 Arbor Biotechnologies, Inc. Reverse transcriptases and gene editing systems comprising such
CN119570793B (zh) * 2024-12-10 2025-08-08 中国农业科学院北京畜牧兽医研究所 一种基因编辑抗病猪分子特征检测方法及应用
CN120702514B (zh) * 2025-09-01 2025-11-07 中科创源(山西)智能科技有限公司 一种视觉模组多轴调节装置

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017173392A1 (en) 2016-03-31 2017-10-05 Two Pore Guys, Inc. Nanopore discrimination of target polynucleotides from sample background by fragmentation and payload binding
JP2017530695A (ja) 2014-08-19 2017-10-19 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸をプロービングおよびマッピングするためのｒｎａ誘導型システム

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4159856A1 (en) * 2015-06-18 2023-04-05 The Broad Institute, Inc. Novel crispr enzymes and systems
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
CA3012607A1 (en) 2015-06-18 2016-12-22 The Broad Institute Inc. Crispr enzymes and systems
CN109153980B (zh) 2015-10-22 2023-04-14 布罗德研究所有限公司 Vi-b型crispr酶和系统
WO2017147345A1 (en) * 2016-02-23 2017-08-31 Arc Bio, Llc Methods and compositions for target detection
US20200263190A1 (en) 2016-04-19 2020-08-20 The Broad Institute, Inc. Novel crispr enzymes and systems
EP3445853A1 (en) 2016-04-19 2019-02-27 The Broad Institute, Inc. Cpf1 complexes with reduced indel activity
CN110382692A (zh) 2016-04-19 2019-10-25 博德研究所 新型crispr酶以及系统
US11174515B2 (en) * 2017-03-15 2021-11-16 The Broad Institute, Inc. CRISPR effector system based diagnostics
CN107488710B (zh) * 2017-07-14 2020-09-22 上海吐露港生物科技有限公司 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒
US10253365B1 (en) * 2017-11-22 2019-04-09 The Regents Of The University Of California Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017530695A (ja) 2014-08-19 2017-10-19 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸をプロービングおよびマッピングするためのｒｎａ誘導型システム
WO2017173392A1 (en) 2016-03-31 2017-10-05 Two Pore Guys, Inc. Nanopore discrimination of target polynucleotides from sample background by fragmentation and payload binding

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHEN Janice S. et al.，CRISPR-Cas12a target binding unleashes single-stranded DNase activity，bioRxiv，2017年11月29日，doi: https://doi.org/10.1101/226993
Molecular Cell，2017年10月，Vol.68，p.15-25

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