MX2022002919A - Nuevas endonucleasas guiadas por rna crispr-cas tipo ii y tipo v de clase 2. - Google Patents

Nuevas endonucleasas guiadas por rna crispr-cas tipo ii y tipo v de clase 2.

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Publication number
MX2022002919A
MX2022002919A MX2022002919A MX2022002919A MX2022002919A MX 2022002919 A MX2022002919 A MX 2022002919A MX 2022002919 A MX2022002919 A MX 2022002919A MX 2022002919 A MX2022002919 A MX 2022002919A MX 2022002919 A MX2022002919 A MX 2022002919A
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MX
Mexico
Prior art keywords
type
crispr
novel class
cas rna
guided endonucleases
Prior art date
Application number
MX2022002919A
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English (en)
Inventor
Carla Alejandra Gimenez
Guillermo Daniel Repizo
Bonnet Federico Alberto Pereyra
Lucía Ana Curti
Franco Goytia
Maria Eugenia Farías
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Science Solutions Llc
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Publication date
Application filed by Science Solutions Llc filed Critical Science Solutions Llc
Publication of MX2022002919A publication Critical patent/MX2022002919A/es

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    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06N—COMPUTING ARRANGEMENTS BASED ON SPECIFIC COMPUTATIONAL MODELS
    • G06N3/00—Computing arrangements based on biological models
    • G06N3/002—Biomolecular computers, i.e. using biomolecules, proteins, cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06N—COMPUTING ARRANGEMENTS BASED ON SPECIFIC COMPUTATIONAL MODELS
    • G06N10/00—Quantum computing, i.e. information processing based on quantum-mechanical phenomena
    • G06N10/40—Physical realisations or architectures of quantum processors or components for manipulating qubits, e.g. qubit coupling or qubit control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Theoretical Computer Science (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Artificial Intelligence (AREA)
  • Software Systems (AREA)
  • Data Mining & Analysis (AREA)
  • Evolutionary Computation (AREA)
  • Computing Systems (AREA)
  • Mathematical Physics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Computational Mathematics (AREA)
  • Pure & Applied Mathematics (AREA)
  • Computational Linguistics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Mathematical Analysis (AREA)
  • Mathematical Optimization (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

En el presente documento se proporcionan nuevas endonucleasas guiadas por RNA CRISPR-Cas de Clase 2 Tipo II y Tipo V y sistemas que las comprenden, por ejemplo, endonucleasas Cas9 y Cas12, y sistemas que las comprenden. También se proporcionan métodos para producirlas y métodos para usarlas. Ejemplos de métodos de uso incluyen la modificación de los DNA diana y la detección de los DNA dirigidos, útiles para aplicaciones terapéuticas y de diagnóstico. Algunas de las aplicaciones diagnósticas pueden utilizar la actividad nucleasa colateral de una enzima unida a una secuencia diana.
MX2022002919A 2019-09-10 2020-09-10 Nuevas endonucleasas guiadas por rna crispr-cas tipo ii y tipo v de clase 2. MX2022002919A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962898340P 2019-09-10 2019-09-10
US202063058448P 2020-07-29 2020-07-29
PCT/US2020/050237 WO2021050755A1 (en) 2019-09-10 2020-09-10 Novel class 2 type ii and type v crispr-cas rna-guided endonucleases

Publications (1)

Publication Number Publication Date
MX2022002919A true MX2022002919A (es) 2022-09-09

Family

ID=72644968

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022002919A MX2022002919A (es) 2019-09-10 2020-09-10 Nuevas endonucleasas guiadas por rna crispr-cas tipo ii y tipo v de clase 2.

Country Status (7)

Country Link
US (1) US20240169179A2 (es)
EP (1) EP4028515A1 (es)
JP (1) JP2022547564A (es)
CN (1) CN114729343A (es)
CA (1) CA3154479A1 (es)
MX (1) MX2022002919A (es)
WO (1) WO2021050755A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020028729A1 (en) 2018-08-01 2020-02-06 Mammoth Biosciences, Inc. Programmable nuclease compositions and methods of use thereof
WO2022221590A1 (en) * 2021-04-15 2022-10-20 Amazon Technologies, Inc. Nucleases for signal amplification
WO2023278461A2 (en) * 2021-06-29 2023-01-05 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Cxc chemokine agonists and antagonists in covid-19 disease and diagnostic assays
WO2023077095A2 (en) * 2021-10-29 2023-05-04 Mammoth Biosciences, Inc. Effector proteins, compositions, systems, devices, kits and methods of use thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2960803T3 (es) * 2012-05-25 2024-03-06 Univ California Métodos y composiciones para la modificación de ADN diana dirigida por RNA y para la modulación de la transcripción dirigida por RNA
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
US11168322B2 (en) * 2017-06-30 2021-11-09 Arbor Biotechnologies, Inc. CRISPR RNA targeting enzymes and systems and uses thereof
US10253365B1 (en) 2017-11-22 2019-04-09 The Regents Of The University Of California Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs

Also Published As

Publication number Publication date
US20220398426A1 (en) 2022-12-15
WO2021050755A1 (en) 2021-03-18
EP4028515A1 (en) 2022-07-20
CN114729343A (zh) 2022-07-08
US20240169179A2 (en) 2024-05-23
JP2022547564A (ja) 2022-11-14
CA3154479A1 (en) 2021-03-18

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