CN116200368A - 一种基于c2c9核酸酶的新型基因组编辑系统及其应用 - Google Patents

一种基于c2c9核酸酶的新型基因组编辑系统及其应用 Download PDF

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CN116200368A
CN116200368A CN202111445474.3A CN202111445474A CN116200368A CN 116200368 A CN116200368 A CN 116200368A CN 202111445474 A CN202111445474 A CN 202111445474A CN 116200368 A CN116200368 A CN 116200368A
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季泉江
陈未中
马佳诚
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ShanghaiTech University
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Abstract

本发明属于生物医药领域，公开了C2C9核酸酶用作RNA引导的核酸内切酶的用途以及一种极小型基因组编辑系统，所述的基因组编辑系统包括C2C9核酸酶和/或编码所述C2C9核酸酶的核酸，以及引导RNA或编码所述引导RNA的核酸。本发明所述极小型基因组编辑系统能够对原核细菌和真核细胞基因组中的至少一个靶序列进行基因编辑。本发明还公开了所述基因组编辑系统的应用以及编辑方法。利用本发明的基因编辑系统或者方法可以将目标基因精准敲除或切断，切割效率高，实现目标基因的精准编辑。

Description

一种基于C2C9核酸酶的新型基因组编辑系统及其应用
技术领域
本发明属于生物医药领域，涉及一种基于极小型C2C9核酸酶的新型基因组编辑系统，以及该系统在原核细菌和真核细胞中的基因组编辑方法和应用。
背景技术
CRISPR/Cas(Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein)是近年来开发的一种新型基因编辑系统。该系统主要由Cas核酸内切酶和相对应的引导RNA所构成。Cas核酸内切酶能够与引导RNA特异性结合，利用碱基互补配对靶向识别并切割基因组特定位点，造成基因组DNA的双链断裂。然后利用生物体内源或外加的DNA修复机制，例如同源重组和非同源重组末端连接的修复机制对断裂的基因组DNA进行修复，从而在修复过程中实现对基因组特定位点的编辑。
利用CRISPR/Cas系统对细胞或者物种进行遗传学操纵，可广泛的应用于生命科学、医学、农学等领域的基础研究、生物技术开发、以及疾病治疗手段研发。例如：对遗传病或癌症等疾病的突变基因进行矫正；对农作物进行基因工程改造，提升其性能；对微生物基因组进行精准改造，推动高附加值化合物生产；对病原微生物基因组进行切割杀死病原微生物，用于感染治疗等。由于CRISPR/Cas系统的简便性与高效性，已经被广泛应用于医学、生物、农学等领域。
目前广泛使用的CRISPR/Cas基因组编辑系统主要包括CRISPR/Cas9和CRISPR/Cas12a两种类型。在这两种类型的基因组编辑系统中，CRISPR效应蛋白核酸酶Cas9和Cas12a均是含有超过1000个氨基酸的大型蛋白，这导致CRISPR/Cas9或CRISPR/Cas12a在向细胞递送过程中存在巨大问题。例如，在活体基因治疗过程中，CRISPR/Cas9或CRISPR/Cas12a系统往往需要被包装入慢病毒载体进行使用。但是慢病毒载体的包装容量有限，这使得CRISPR/Cas9和CRISPR/Cas12a系统的巨大分子尺寸大大限制了慢病毒载体包装效率，增加了包装难度，极大限制这些系统在基因治疗等领域的广泛应用。此外，由于CRISPR/Cas系统识别的原间隔区-相邻基序(PAM、PAM序列或PAM位点)的有限性，使得能够选择的基因组位点受到限制，影响了其在医学和生物学等领域的深入应用。因此，发现和开发新型高效并且小分子量的基因组编辑系统显得尤为迫切。
发明内容
本发明提供一种极小型基因组编辑系统(包括CRISPR/C2C9系统)及方法。利用本发明的基因组编辑系统可以对原核细菌和真核细胞的目标位点进行精确的切割，从而实现基因的精准敲除或者编辑。本发明还提供了新颖的RNA-可编程的内切核酸酶。本发明还涉及其它组分，包括引导RNA(gRNA)和/或靶序列，以及在各种应用中产生和使用它们的方法。这样的应用的例子包括用于调节转录的方法，以及使用新颖的核酸酶和其它组分诸如核酸和/或多肽来靶向、编辑和/或操纵基因(如DNA或RNA)的方法。本发明还涉及包含所述编辑系统元件的重组细胞和试剂盒等。
本发明一方面提供了一种对基因组中的至少一个靶序列进行基因编辑的基因组编辑系统，其含有：
(a)C2C9核酸酶或编码C2C9核酸酶的核酸；和
(b)引导RNA(gRNA)或编码所述gRNA的核酸。
所述C2C9核酸酶和与之匹配的引导RNA形成复合体。
在一优选实施方式中，所述基因组编辑系统为CRISPR-C2C9基因组编辑系统。
在一优选实施方式中，所述CRISPR-C2C9基因组编辑系统含有：
(1)C2C9核酸酶的表达构建体；
(2)完整引导RNA的表达构建体；
所述完整引导RNA包含引导RNA骨架，以及在该引导RNA骨架的3’末端的添加的靶向序列所对应的RNA序列；所述靶向序列为PAM序列之后长度为12～40bp的核酸片段，优选为PAM序列之后长度为20bp的核酸片段。本发明中所述的C2C9核酸酶可为本领域常规的C2C9核酸酶。优选地，所述C2C9核酸酶的大小优选不超过800个氨基酸。更优选地，所述C2C9核酸酶来源为下列氨基酸序列之任一或与下列任一氨基酸序列具有至少80％序列同源性：SEQ ID NO.3-117。在一些实施方式中，所述C2C9核酸酶选自ActinomaduracraniellaeC2C9(AcC2C9)、Corynebacterium glutamicumC2C9(CgC2C9)、RothiadentocariosaC2C9(RdC2C9)以及Micrococcus luteusC2C9(MiC2C9)等构成的群组。
在本发明较佳实施例中，所述的C2C9核酸酶为如序列表中SEQ ID NO.3所示的AcC2C9核酸酶。
在某些实施方式中，编码所述AcC2C9核酸酶的核酸是脱氧核糖核酸(DNA)。在某些实施方式中，AcC2C9核酸酶的DNA编码序列如序列表中SEQ ID NO.121所示或与SEQ ID NO:121具有至少50％，至少55％，至少60％，至少65％，至少70％，至少75％，至少80％，至少85％，至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、至少99％序列同源性的变体。
在某些实施方式中，编码所述AcC2C9核酸酶的核酸是核糖核酸(RNA)。在某些实施方式中，编码所述C2C9核酸酶的RNA是mRNA。
在一些实施方式中，编码所述AcC2C9核酸酶的序列为针对人类密码子优化后的编码序列，较佳地如序列表中SEQ ID NO.122所示或与SEQ ID NO:122具有至少50％，至少55％，至少60％，至少65％，至少70％，至少75％，至少80％，至少85％，至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、至少99％序列同源性的变体。
在某些实施方式中，将所述C2C9核酸酶或编码C2C9核酸酶的核酸配制在脂质体或脂质纳米颗粒中。在某些实施方式中，所述脂质体或脂质纳米颗粒也包含所述gRNA或编码所述gRNA的核酸。
在某些实施方式中，所述系统包含与gRNA预络合的C2C9核酸酶，从而形成核糖核蛋白(RNP)复合物，其识别靶基因(如靶DNA)序列上的PAM序列；即精确定位DNA序列侧翼包括C2C9核酸酶和引导RNA形成的复合体识别靶基因上的PAM序列。
其中，所述AcC2C9核酸酶的引导RNA骨架序列如序列表中SEQ ID NO.120所示。
在一些实施方式中，所述的完整引导RNA表达构建体包含在引导RNA骨架的表达DNA序列，以及在该表达DNA序列的3’末端添加的靶向序列相对应的RNA序列的表达DNA序列；其中，所述靶向序列为PAM序列之后长度为20bp的片段。
其中，所述CRISPR-C2C9基因组编辑系统优选识别的PAM位点为AAN和/或GAN。
在某些实施方式中，本发明所述系统还可以进一步包括包含异源多核苷酸序列的供体模板，其中所述异源多核苷酸序列能够被插入所述靶多核苷酸序列中。在某些实施方式中，异源多核苷酸供体模板与gRNA物理连接。
在另一个方面，本发明提供了一种靶向、编辑、修饰或操纵靶基因座处的双链DNA的方法，所述方法包括将如上所述系统中的C2C9核酸酶、完整引导RNA以及所述双链DNA混合；所述的完整引导RNA为：5’-与所述的C2C9核酸酶相应的引导RNA骨架+所述双链DNA上的靶向序列相对应的RNA序列-3’。在一些实施方式中，所述方法用于切割双链DNA。
在一些实施方式中，本发明提供了在细胞中进行如上靶向、编辑、修饰或操纵靶基因座处的双链DNA的方法。在一些实施方式中，所述方法还进一步包括将多核苷酸供体模板引入细胞中的步骤，所述供体模板可以是供体单链或双链多核苷酸供体模板。
在本发明一些实施方式中，通过同源性指导的修复、非同源末端连接或微同源性介导的末端连接在DSBs处修复DNA。
如上所述，与所述的AcC2C9核酸酶相应的优选引导RNA骨架如序列表中SEQ IDNO.120所示。其3’端加上针对不同靶序列的靶向RNA序列即可以形成完整引导RNA。
对所述的C2C9核酸酶的优选限定如上所述，较佳地来源为：SEQ ID NO.3-117等构成的群组；较佳地，选自Actinomadura craniellae C2C9(AcC2C9)、Corynebacteriumglutamicum C2C9(CgC2C9)、Rothia dentocariosa C2C9(RdC2C9)以及Micrococcusluteus C2C9(MiC2C9)；更佳地如序列表中SEQ ID NO.3或其变体所示。所述AcC2C9核酸酶的针对人类密码子优化后更佳的编码序列如序列表中SEQ ID NO.122所示。所述的变体与SEQ ID NO.3的氨基酸残基具有至少20％(如30％、40％、50％、60％、70％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％)的序列同源性并保留受RNA引导的DNA结合活性和/或双链DNA切割活性。
本发明中所述的切割方法可为本领域常规，例如，在本发明较佳实施例中：所述的切割方法在150mM NaCl，10mM MgCl2，10mM Tris-HCl,pH＝7.5，1mM DTT的缓冲溶液中进行。
所述切割方法的反应温度较佳地为37℃、反应时间较佳地为30-60min。
在另一个方面，本发明提供了一种基因组编辑方法，其包括将如上所述的基因组编辑系统导入到含有靶序列的原核细菌或真核细胞内进行基因组编辑。
本发明中所述的原核细菌包括但不限于大肠杆菌、肺炎克雷伯氏菌等原核微生物。
本发明中所述的真核细胞包括但不限于哺乳动物细胞、酵母等真核生物细胞。
如上所述的基因组编辑方法中的“基因编辑”可为本领域常规，例如基因切割、基因删除、基因插入、点突变、转录抑制、转录激活、碱基编辑和引导编辑等，但不限于此。
在另一个方面，本发明提供了C2C9核酸酶在基因组编辑中的应用。
对所述的C2C9核酸酶以及所述的基因编辑的进一步限定如上所述。
该基因组编辑系统的核酸酶的大小不超过800个氨基酸。
在另一个方面，本发明提供了一种经修饰的细胞，其通过上文所述的任一个系统或方法编辑细胞的基因组获得。在一实施方式中，所述经修饰的细胞包含:(a)根据上文所述的C2C9核酸酶或编码所述C2C9核酸酶的核酸；和(b)引导RNA(gRNA)或编码所述gRNA的核酸，其中所述gRNA能够将所述C2C9核酸酶或其变体指导至靶多核苷酸序列。在某些实施方式中，所述经修饰的细胞进一步含有包含异源多核苷酸序列的供体模板，其中所述异源多核苷酸序列能够被插入所述靶多核苷酸序列中。
本发明的积极进步效果包括：
CRISPR/C2C9为申请人发现的一种全新的基因组编辑系统，包括C2C9核酸酶和gRNA，该系统具有尺寸小(C2C9核酸酶一般不超过甚至小于800个氨基酸)，不需要复杂的PAM(识别PAM位点为AAN等特点)。通过完整引导RNA的导向和定位功能，C2C9核酸酶能够体内、离体细胞或无细胞环境中精确定位、靶向、并切割基因组DNA，实现基因组DNA双链断裂。利用宿主细胞自身或外源补充的修复机制，该系统能够高效和精确地实现活体内或离体细胞或无细胞环境中的基因组编辑。
附图说明
图1为AcC2C9的PAM的鉴定流程及结果图。结果显示：AcC2C9能有效识别5’-AAN类型的PAM序列以及少量5’-GAN序列(其中N为简并碱基，代表A、T、C或G任意一种碱基)。其中，横坐标-1、-2、-3、-4、-5、-6分别代表NTS链(非靶向链)上位于靶向-DNA区段序列5’端上游的第1、第2、第3、第4、第5、第6个DNA碱基；其上的字母代表AcC2C9核酸酶对DNA序列的偏好性，字母所占的比重越大，代表AcC2C9对该碱基的偏好性越强。
图2为采用CRISPR-AcC2C9系统在肺炎克雷伯氏菌活细胞中进行基因敲除的PCR结果图。结果显示，选定的dha基因可以被CRISPR-AcC2C9系统精准敲除。
图3为采用AcC2C9核酸酶切割底物DNA的结果图。结果显示：底物DNA被AcC2C9精准切断成两段DNA片段。
图4(A)AcC2C9完整引导RNA的序列示意图。(B)tracrRNA和crRNA条件下，用AcC2C9核酸酶切割底物DNA的结果图。
图5为采用AcC2C9核酸酶切割四种58bp的荧光标记DNA底物的结果图。结果显示：AcC2C9在靶向链的主要切割位点为PAM位点3’端下游的21-22nt处，而在非靶向链的主要切割位点为PAM位点3’端下游的15-16nt处。
图6为AcC2C9瞬时表达质粒介导的人细胞中的基因编辑结果图。(A)为Indel实验的TBE-PAGE胶图。在所选的5个不同基因位点中，AcC2C9核酸酶成功实现了对VEGFA、HEXA和DNMT1三个基因的高效基因编辑。(B)为AcC2C9瞬时表达质粒介导的人细胞中VEGFA基因编辑后高通量测序的统计结果图。如图所示，AcC2C9核酸酶可精确在靶序列处引入插入或缺失突变。
具体实施方式
下面结合具体实施例，进一步阐述本发明。应理解，这些实施例仅用于说明本发明而不用于限制本发明的范围。此外应理解，在阅读了本发明讲授的内容之后，本领域技术人员可以对本发明作各种改动或修改，这些等价形式同样落于本申请所附权利要求书所限定的范围。
术语“C2C9”、“C2C9核酸酶”、“C2C9多肽”、“C2C9蛋白”、“C2C9蛋白质”可互换使用。
术语“引导RNA”、“gRNA”、“单一gRNA”和“嵌合gRNA”可互换使用。
需要注意的是，该发明中的术语“一”或“一个”实体是指一个或多个该实体；因此，术语“一”(或“一个”)，“一个或多个”和“至少一个”在本文中可互换使用。
术语“同源性”或“同一性”或“相似性”是指两个肽之间或两个核酸分子之间的序列相似性。同源性可以通过比对不同多肽或核酸分子中的相对应位置来确定，当被比较分子序列中的同一位置在不同序列中被相同的碱基或氨基酸占据时，那么该分子在该位置是同源的。序列之间的同源程度由序列共有的匹配或同源位置的数目的函数决定。“不相关的”或“非同源的”序列与本发明所公开的序列之一的同源性应小于20％。
多核苷酸或多核苷酸区域(或多肽或多肽区域)与另一多核苷酸或多核苷酸区域(或多肽或多肽区域)具有一定百分比的序列同源性(例如，20％、30％、40％、50％、60％、70％、80％、90％、95％、98％或99％)是指比对时，被比对的两个序列中有该百分比的碱基(或氨基酸)是相同的。该比对和百分比同源性或序列同一性可以使用本领域已知的软件程序和方法来确定。例如像Ausubel et al.eds.(2007)Current Protocols in MolecularBiology中描述的那样。优选地，序列比对时应使用默认参数。其中一个备选的对齐程序是BLAST，使用默认参数。特别地，当使用程序BLASTN和BLASTP进行比对时，使用以下默认参数：Genetic code＝standard；filter＝none；strand＝both；cutoff＝60；expect＝10；Matrix＝BLOSUM62；Descriptions＝50sequences；sort by＝HIGH SCORE；Databases＝non-redundant，GenBank+EMBL+DDBJ+PDB+GenBank CDS translations+SwissProtein+SPupdate+PIR。在生物学意义上被认为是等价的多核苷酸是指具有上述限定百分比同源性并编码具有相同或相似生物学活性的多肽的那些多核苷酸。
当多核苷酸为DNA时，该多核苷酸的序列由以下四个核苷酸碱基的代表字母组成：腺嘌呤(A)、胞嘧啶(C)、鸟嘌呤(G)以及胸腺嘧啶(T)。当多核苷酸为RNA时，该多核苷酸的序列由以下四个核苷酸碱基的代表字母组成：腺嘌呤(A)、胞嘧啶(C)、鸟嘌呤(G)以及尿嘧啶(U)。因此，术语“多核苷酸序列”是多核苷酸分子的字母表示。该字母表示可以输入到具有中央处理单元的计算机中的数据库中，并用于生物信息学应用，例如功能基因组学和同源性搜索。术语“多态性”是指多于一种形式的基因或其部分的共存，“基因的多态性区域”是指基因在同一位置有不同的核苷酸表现形式(即不同的核苷酸序列)。基因多态性区域可以是单核苷酸，其在不同的等位基因中是不同的。
在本发明中，术语“多核苷酸”和“寡核苷酸”可互换使用，并且它们是指任何长度的核苷酸的聚合形式，无论是脱氧核糖核苷酸还是核糖核苷酸或其类似物。多核苷酸可以具有任何三维结构并且可以执行已知或未知的任何功能。多核苷酸的实例包括但不限于如下这些：基因或基因片段(包括探针，引物，EST或SAGE标签)，外显子，内含子，信使RNA(mRNA)，转运RNA，核糖体RNA，核酶，cDNA，dsRNA，siRNA，miRNA，重组多核苷酸，分支多核苷酸，质粒，载体，任何序列的分离的DNA，任何序列的分离的RNA，核酸探针和引物。多核苷酸也包含经过修饰的核苷酸，例如甲基化的核苷酸和核苷酸类似物。如果多核苷酸上存在修饰，该修饰可以在组装多核苷酸之前或之后赋予。核苷酸序列可以被非核苷酸组分打断。多核苷酸可以在聚合后被进一步修饰，例如通过偶联被标记组分所标记。该术语同时指双链和单链多核苷酸分子。除非另有说明或要求，否则本发明公开的多核苷酸的任何实施方案包括其双链形式和已知或预测能构成双链形式的两条互补单链形式中的任一种。
当将其应用于多核苷酸时，术语“编码”是指多核苷酸“编码”多肽，意即在其天然状态下或当通过本领域技术人员公知的方法操作时，其可以通过转录和/或翻译以产生目的多肽和/或其片段，或产生能够编码该目的多肽和/或其片段的mRNA。反义链是指与该多核苷酸互补的序列，并且可以从中推导出编码序列。
术语“基因组DNA”表示生物的基因组的DNA，包括细菌、古细菌、真菌、原生生物、病毒、植物或动物的基因组的DNA。
术语“操纵”DNA包括结合、在一条链产生切口、或切割DNA的两条链，或包括修饰或编辑DNA或与DNA结合的多肽。操纵DNA可以沉默、激活或调节由所述DNA编码的RNA或多肽的表达(使其不转录，或降低转录活性，或使其不翻译，或降低翻译水平)，或防止或增强多肽与DNA的结合。切割可通过多种方法进行，例如磷酸二酯键的酶促或化学水解；可单链切割或双链切割；DNA切割可导致平头末端或交错末端的产生。
术语“可杂交的”或“互补的”或“基本上互补的”是指，核酸(例如RNA)包含核苷酸序列，该序列使得其能够在适当的体外和/或体内温度和溶液离子强度条件下以序列特异性的、反平行的方式与另一核酸非共价地结合，即，形成沃森-克里克碱基对和/或G/U碱基对、“退火”或“杂交”。
在本领域中可以理解，多核苷酸的序列不需要与其可特异性杂交的靶核酸的序列100％互补。多核苷酸可在一个或多个区段上杂交。多核苷酸可包含与其所靶向的靶核酸序列内的靶区域的至少70％、至少80％、至少90％、至少95％、至少99％或100％的序列互补性。本领域中，可以使用已知的BLAST程序和PowerBLAST程序，常规地确定核酸内特定核酸序列段之间的百分比互补性。
术语“肽”、“多肽”和“蛋白”在本发明中可互换地使用，且表示任何长度的氨基酸的聚合形式，其可以包括编码和非编码的氨基酸，化学或生物化学修饰或衍生的氨基酸，和具有修饰的肽主链的多肽。
术语“结合结构域”是指能够非共价地结合另一分子的蛋白结构域。结合结构域可以结合至例如DNA分子(DNA-结合蛋白)、RNA分子(RNA-结合蛋白)和/或蛋白分子(蛋白-结合蛋白)。在蛋白结构域-结合蛋白的情况下，它可以结合它本身(以形成同二聚体、同三聚体等)和/或它可以结合一种或多种不同蛋白质的一个或多个分子。
术语“保守的氨基酸置换”表示蛋白中具有相似侧链的氨基酸残基的可互换性。示例性的保守氨基酸置换组是：缬氨酸-亮氨酸-异亮氨酸、苯丙氨酸-酪氨酸、赖氨酸-精氨酸、丙氨酸-缬氨酸和天冬酰胺谷氨酰胺。
术语“编码”特定RNA的DNA序列是转录为RNA的DNA核酸序列。DNA多核苷酸可以编码被翻译成蛋白的RNA(mRNA)，或者DNA多核苷酸可以编码不被翻译成蛋白质的RNA(例如，tRNA、rRNA或gRNA；也称为“非编码”RNA或“ncRNA”)。“蛋白编码序列”或编码特定蛋白或多肽的序列是在合适调节序列的控制下，在体内或体外转录成mRNA(在DNA的情况下)并翻译(在mRNA的情况下)成多肽的核酸序列。
术语“载体”或“表达载体”是复制子，诸如质粒、噬菌体、病毒或粘粒，可以在其上附着另一种DNA区段，即，“插入片段”，以便实现附着的区段在细胞中的复制。
术语“表达盒”包含可操作地连接至启动子的DNA编码序列。“可操作地连接的”表示并列连接，各组分处于允许它们以其预期方式起作用的关系中。术语“重组表达载体”或“DNA构建体”在本发明中互换地用于表示包含载体和至少一个插入片段的DNA分子。重组表达载体通常是为了表达和/或扩增插入片段的目的或为了构建其它重组核苷酸序列而产生。
当外源DNA例如重组表达载体已经被引入细胞内时，细胞已被所述DNA“遗传上修饰”或“转化”或“转染”。外源DNA的存在导致永久或短暂的遗传改变。转化DNA整合或不整合到细胞的基因组中。
术语“靶DNA”是包含“靶位点”或“靶序列”的DNA多核苷酸。术语“靶位点”、“靶序列”、“靶原间隔区DNA”或“原间隔区样序列”在本发明中互换地用于表示存在于靶DNA中的核酸序列，如果存在足够的用于结合的条件，则gRNA的DNA-靶向区段将与其结合。RNA分子包含与靶DNA内的靶序列结合、杂交或互补的序列，从而将结合的多肽靶向至靶DNA内的特定位置(靶序列)。“切割”是指DNA分子的共价主链的断裂。
术语“核酸酶”和“内切核酸酶”可互换使用，用于指具有用于多核苷酸切割的内切核酸降解催化活性的酶。核酸酶的“切割结构域”或“活性结构域”或“核酸酶结构域”是指在核酸酶内具有用于DNA切割的催化活性的多肽序列或结构域。切割结构域可以包含在单个多肽链中，或者切割活性可以由两个或多个多肽的缔合产生。
术语“定位多肽”或“RNA-结合位点指导的多肽”是指结合RNA并靶向至特定DNA序列的多肽。
术语“引导序列”或DNA-靶向区段(或“DNA-靶向序列”)包含与在本发明中称为“原间隔区样”序列的靶DNA内的特定序列互补的核苷酸序列(靶DNA的互补链)。
术语“重组”是指在两个多核苷酸之间交换遗传信息的过程。本发明中使用的“同源性指导的修复(HDR)”表示例如在细胞中双链断裂修复期间发生的特殊化形式的DNA修复。这个过程需要核苷酸序列同源性，使用“供体”分子为“靶”分子(即，经历双链断裂的分子)的修复提供模板，并导致遗传信息从供体转移至靶。如果供体多核苷酸不同于靶分子，并且供体多核苷酸的部分或全部序列被并入到靶DNA中，则同源性指导的修复可能导致靶分子序列的改变(例如插入、缺失、突变)。
术语“非同源末端连接(NHEJ)”是指在不需要同源模板的情况下通过将断裂末端彼此直接连接来修复DNA中的双链断裂。NHEJ经常导致双链断裂位点附近的核苷酸序列缺失。
术语“治疗”包括防止疾病或症状的发生；抑制疾病或症状或减轻疾病。
术语“个体”、“受试者”、“宿主”和“患者”在本发明中可互换地使用，并且表示期望对其进行诊断、治疗或疗法的任何哺乳动物受试者，特别是人。
本发明提供了一种适用于CRISPR编辑系统的极小型C2C9核酸酶；包含：i)与gRNA相互作用的RNA-结合部分；以及活性部分。
所述C2C9核酸酶具有以下至少一种活性：调节靶基因(例如靶DNA)内的转录，切割活性(核糖核酸内切酶和/或核酸内切酶活性)、基因编辑活性等，实现基因编辑的类型包括但不限于：基因切割、基因删除、基因插入、点突变、转录抑制、转录激活和碱基编辑等。所述C2C9核酸酶可以来源于任何生物物种。
作为非限制性的举例说明，所述C2C9核酸酶可以作为例如CRISPR编辑系统的核酸酶。
本发明中，还可以通过修饰、突变、DNA改组等方式形成C2C9核酸酶变体，使得C2C9核酸酶变体具有改善的所期望的特征，例如功能、活性、动力学、半衰期等。所述修饰例如可以是氨基酸的缺失、插入或取代，再例如可以是用来自不同核酸酶的同源或异源切割结构域(例如CRISPR-相关核酸酶的HNH结构域)置换C2C9核酸酶的“切割结构域”)；通过本领域已知的DNA结合和/或DNA修饰蛋白的任何修饰方法，如甲基化作用、脱甲基作用、乙酰化作用等，例如可以改变C2C9核酸酶的DNA靶向性。所述DNA改组是指在不同来源的C2C9核酸酶的DNA序列之间交换序列片段，以产生编码具有RNA-指导的内切核酸酶活性的合成蛋白的嵌合DNA序列。所述修饰、突变、DNA改组等可以是单一使用或组合使用。
所述的C2C9核酸酶从N端到C端包含：
结构域1，其包含如SEQ ID NO.1所示的氨基酸序列或与该氨基酸序列具有至少50％，至少55％，至少60％，至少65％，至少70％，至少75％，至少80％，至少85％，至少90％序列同一性的变体；
结构域2，其包含如SEQ ID NO.2所示的氨基酸序列或与该氨基酸序列具有至少50％，至少55％，至少60％，至少65％，至少70％，至少75％，至少80％，至少85％，至少90％序列同一性的变体。
所述C2C9核酸酶变体可以是：
(I)与野生型C2C9核酸酶的氨基酸序列具有至少20％(如30％、40％、50％、60％、70％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％)的序列同源性，并保留野生型C2C9核酸酶的活性的变体；
(II)根据(I)的C2C9核酸酶或其变体，其进一步包含其他组分，例如核定位信号片段，以使得构建的C2C9 CRISPR系统在无细胞反应、原核细胞、或真核细胞环境中具有适当活性；
(III)编码野生型C2C9核酸酶或根据(I)和(II)的变体的对应多核苷酸序列的密码子优化变体；
(IV)根据野生型C2C9核酸酶以及(I)至(III)中的任一项的变体，其进一步包含：
(a)一种或多种修饰或突变，其产生具有显著减小的或不可检测的核酸酶活性的C2C9；和
(b)具有其他功能活性的多肽或结构域。
在(IV)(b)下的合适多肽与C2C9核酸酶及其变体的C-端结构域连接。
在(IV)下的C2C9核酸酶变体可以通过修饰或突变将任何碱基对转化为任何可能的其它碱基对，而不是在靶DNA序列中引入双链断裂。
在(IV)下的C2C9核酸酶变体可以是野生型C2C9核酸酶或(I)至(III)的变体与异源序列形成的融合体或嵌合多肽。所述异源序列包括但不限于是光诱导的转录调节物、小分子/药物应答转录调节物、转录因子、转录阻遏蛋白等。在形成融合体或嵌合多肽时，野生型C2C9核酸酶或(I)至(III)的变体可以是完整或部分或完全缺陷的C2C9核酸酶。例如，含有催化活性的内切核酸酶结构域的C2C9核酸酶与Fokl结构域融合，形成嵌合蛋白；或者已通过修饰失去内切核酸酶结构域活性的C2C9核酸酶与Fokl结构域融合，形成嵌合蛋白；或者，外遗传修饰因子、标签、成像剂、转录调节剂、组蛋白、调节基因结构或活性的其他组分等与C2C9核酸酶融合以制备嵌合蛋白。在一些实施方式中，异源序列可以提供用于易于追踪或纯化的标签(例如荧光蛋白，例如绿色荧光蛋白(GFP)、YFP、RFP、CFP等；His标签；血凝素(HA)标签；FLAG标签；Myc标签等)。在一些实施方式中，异源序列可以提供C2C9核酸酶的亚细胞定位。
在一些实施方式中，C2C9核酸酶变体为C2C9核酸酶的修饰形式。在一些实施方式中，C2C9核酸酶的修饰形式包含降低C2C9核酸酶的天然存在的核酸酶活性的氨基酸变化。例如，在一些实施方式中，C2C9核酸酶的修饰形式具有小于50％、小于40％、小于30％、小于20％、小于10％、小于5％或小于1％的相应野生型C2C9核酸酶的核酸酶活性。在一些实施方式中，C2C9核酸酶的修饰形式没有显著的核酸酶活性，但是保留了与gRNA相互作用的能力。在一些实施方式中，C2C9核酸酶的修饰形式没有核酸酶活性。在一些实施方式中，C2C9核酸酶的内切核糖核酸酶活性或对DNA的结合亲和力在没有相当大地减少或增强的情况下，具有改变的或取消的DNA内切核酸酶活性的突变多肽。
在一些实施方式中，所述C2C9可以通过与其他酶成分或其他成分结合使用，以进一步开发C2C9核酸酶的各种潜在应用。作为(IV)下的C2C9核酸酶变体非限制性的例子，例如是通过融合失活的C2C9和碱基脱氨酶开发基于C2C9核酸酶的单碱基编辑系统；通过融合失活的C2C9和逆转录酶开发基于C2C9核酸酶的Prime编辑系统；通过融合失活的C2C9和转录激活因子，开发基于C2C9核酸酶的转录激活系统；通过融合失活的C2C9和核酸表观修饰酶，开发基于C2C9核酸酶的表观修饰系统；利用失活的C2C9，开发基于C2C9核酸酶的转录抑制系统。
C2C9核酸酶变体可以具有如下特定性质，包括但不限于：
具有增强的或降低的与靶位结合的能力，或保留了与靶位结合的能力；
具有增强的或降低的核糖核酸内切酶和/或核酸内切酶活性，或保留了核糖核酸内切酶和/或核酸内切酶活性；
具有脱氨酶活性，其可作用于胞嘧啶、鸟嘌呤或腺嘌呤碱基，并随后通过脱氨基位点复制并在细胞内修复，分别产生鸟嘌呤、胸腺嘧啶和鸟嘌呤；
具有调节靶DNA的转录的活性，可以是增加也可以是减少靶DNA中特定位置处的靶DNA转录；
具有改变的DNA靶向性；
增加或降低或维持的稳定性；
可以切割靶DNA的互补链，但具有降低的切割靶DNA的非互补链的能力；
可以切割靶DNA的非互补链，但具有降低的切割靶DNA的互补链的能力；
具有降低的切割靶DNA的互补链和非互补链两者的能力；
具有修饰与DNA相关的多肽(例如组蛋白)的酶活性，酶活性可以是甲基转移酶活性、脱甲基酶活性、乙酰转移酶活性、脱乙酰酶活性、激酶活性、磷酸酶活性、泛素连接酶活性、脱泛素活性、核糖基化活性等中的一种或几种(通过这些酶活性催化对蛋白的共价修饰；例如，C2C9核酸酶变体通过甲基化作用、乙酰化作用、泛素化、磷酸化作用等，修饰组蛋白，以引起组蛋白相关DNA的结构变化，从而控制DNA的结构和特性)。
在一些实施方式中，C2C9核酸酶变体无切割活性。在一些实施方式中，C2C9核酸酶变体具有单链切割活性。在一些实施方式中，C2C9核酸酶变体具有双链切割活性。
具有增强的活性或能力是指，相对于野生型C2C9核酸酶，具有提升至少1％、5％、10％、20％、30％、40％、50％的活性或能力。
具有降低的活性和能力是指相对于野生型C2C9核酸酶，具有小于50％、小于40％、小于30％、小于20％、小于10％、小于5％或小于1％的活性或能力。
本发明中，所述C2C9核酸酶较佳地来源于SEQ ID NO.3-117等构成的群组。
在一些实施方式中，为了利用不同C2C9核酸酶的各种酶学特性(例如，针对不同的PAM序列偏好)，不同物种来源的C2C9核酸酶有利于提供不同应用，例如，增加或减少酶促活性；增加或减少细胞毒性水平；改变NHEJ，同源性定向修复，单链断裂，双链断裂等之间的平衡。
在某些实施方式中，所述C2C9核酸酶来自Actinomadura craniellae，其包含SEQID NO:3的氨基酸序列或其与SEQ ID NO:3具有至少约90％(诸如至少约91％、92％、93％、94％、95％、96％、97％、98％、99％或更多中的任一种)序列同源性的变体；Actinomaduracraniellae C2C9或“AcC2C9”会识别PAM序列AAN。在某些实施方式中，所述C2C9核酸酶来自Corynebacterium glutamicum(SEQ ID NO:5)，Corynebacteriumglutamicum C2C9或“CgC2C9”会识别PAM序列AAN。在某些实施方式中，所述C2C9核酸酶来自Rothia dentocariosa(SEQ ID NO:20)，Rothia dentocariosa C2C9或“RdC2C9”会识别PAM序列AAG。在某些实施方式中，所述C2C9是来自Micrococcus luteus(SEQ ID NO:12)，Micrococcus luteus C2C9或“MiC2C9”会识别PAM序列AAN。所述AAN包含AAA、AAC、AAG和AAT。
来自不同物种的C2C9核酸酶或来自同一物种的不同C2C9核酸酶可能在靶DNA中需要不同的PAM序列，因此，对于选择的特定C2C9核酸酶，PAM序列要求可能与前述PAM序列不同。
在一些实施方式中，这些小的C2C9可用作本发明下文所述的任何系统、组合物、试剂盒和方法中。
在一优选实施方式中，所述C2C9核酸酶来源于Actinomadura craniellae，为AcC2C9，其序列如序列表中SEQ ID NO.3所示。
在一些实施方式中，所述AcC2C9核酸酶变体是：
(I)与SEQ ID NO.3的野生型AcC2C9核酸酶的氨基酸序列具有至少20％(如30％、40％、50％、60％、70％、80％、85％、90％、95％、99％)的序列同源性，并且相对于野生型C2C9核酸酶具有增加、减少或保留的活性的变体；
(II)根据(I)的AcC2C9核酸酶或其变体，其进一步包含其他组分，例如核定位信号，以使得构建的C2C9 CRISPR系统在无细胞反应、原核细胞、或真核细胞环境中具有适当活性；
(III)编码SEQ ID NO.3野生型AcC2C9核酸酶或根据(I)和(II)的变体的对应多核苷酸序列的密码子优化变体；
(IV)根据SEQ ID NO.3野生型AcC2C9核酸酶以及(I)至(III)中的任一项的变体，其进一步包含：
(a)一种或多种修饰或突变，其产生具有显著减小的或不可检测的核酸酶活性的C2C9；和
(b)具有其他功能活性的多肽。
本发明提供的C2C9核酸酶的氨基酸个数少。在一优选实施方式中，当所述C2C9核酸酶氨基酸序列如SEQ ID NO:3所示时，其与PAM序列AAN相互作用。切割位点通常存在于PAM序列上游的一到三个碱基对内。来自不同物种的C2C9核酸酶或来自同一物种的不同C2C9核酸酶可能在靶DNA中需要不同的PAM序列，因此，对于选择的特定C2C9核酸酶，PAM序列要求可能与前述PAM序列不同；C2C9可以通过工程改造成靶向PAM序列“AAN”或其他合适的靶向PAM序列。
在一些实施方式中，所述AcC2C9核酸酶变体与SEQ ID NO:3所示的氨基酸序列具有至少90％(诸如至少约91％、92％、93％、94％、95％、96％、97％、98％、99％)序列同源性并保留AcC2C9核酸酶的活性。
如果没有另外说明，术语“C2C9”、“C2C9核酸酶”包括野生型C2C9核酸酶以及其所有变体，本领域技术人员可以通过常规手段可以确定C2C9核酸酶变体的类型，而不受限于上文所例举的那些。
本发明还提供了一种包含编码AcC2C9核酸酶的核苷酸的核酸。在一实施方式中，本发明提供了密码子优化的多核苷酸序列，其编码一个或多个功能性C2C9结构域，或编码具有与原始天然核苷酸序列编码的多肽相同的功能的多肽。因此，本领域技术人员可以为在特定目标物种中使用本发明所述的C2C9核酸酶，对核苷酸序列或重组核酸进行密码子优化。可以通过本领域已知的其他方法用于密码子优化，其与天然核苷酸序列SEQ ID NO:121具有小于100％同一性(例如，小于80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％)。本发明的多核苷酸经密码子优化以增加编码的C2C9核酸酶在靶细胞中的表达。在某些实施方式中，本发明的多核苷酸经密码子优化以增加在人细胞中的表达。在某些实施方式中，本发明的多核苷酸经密码子优化以增加在大肠杆菌细胞中的表达。在一些实施方式中，本发明的多核苷酸经密码子优化以增加在真菌细胞中的表达。在某些实施方式中，本发明的多核苷酸经密码子优化以增加在昆虫细胞中的表达。
在一实施方式中，编码所述AcC2C9核酸酶的核酸如序列表中SEQ ID NO.121所示。在一实施方式中，本发明提供了AcC2C9核酸酶密码子优化的多核苷酸序列，与SEQ ID NO:121具有至少90％、92％、93％、94％、95％、96％、97％、98％、99％、99.2％、99.5％、99.8％、99.9％或100％序列同源性。在一优选实施方式中，所述AcC2C9核酸酶的针对人类密码子优化后更佳的编码序列如序列表中SEQ ID NO.122所示，其编码一个或多个功能性C2C9结构域，或编码具有与原始天然核苷酸序列编码的多肽相同的功能的多肽。在一些实施方式中，所述编码C2C9核酸酶的多核苷酸与SEQ ID NO:121-122具有至少约90％(诸如至少约91％、92％、93％、94％、95％、96％、97％、98％、99％或更多中的任一种)序列同源性。
在某些实施方式中，编码C2C9核酸酶的核酸为DNA。在某些实施方式中，编码C2C9核酸酶的核酸为RNA。在某些实施方式中，编码C2C9核酸酶的核酸是表达载体，例如重组表达载体。可以使用任何合适的表达载体，只要它与宿主细胞相容即可，包括但不限于，病毒载体(例如基于痘苗病毒的病毒载体；脊髓灰质炎病毒；腺病毒；腺相关病毒；SV40；单纯疱疹病毒；人免疫缺陷病毒；反转录病毒载体(例如鼠白血病病毒，脾脏坏死病毒，以及衍生自反转录病毒的载体，例如劳斯肉瘤病毒，Harvey肉瘤病毒，禽类白血病病毒，慢病毒，人免疫缺陷病毒，骨髓增生肉瘤病毒和乳腺肿瘤病毒)等。
在某些实施方式中，编码C2C9核酸酶的核苷酸序列可操作地连接至控制元件，例如转录控制元件，例如启动子。在某些实施方式中，编码C2C9核酸酶的核苷酸序列可操作地连接至诱导型启动子。在某些实施方式中，编码C2C9核酸酶的核苷酸序列可操作地连接至组成型启动子。转录控制元件可以在真核细胞，例如哺乳动物细胞；或原核细胞(例如细菌或古细菌细胞)中起作用。在某些实施方式中，编码C2C9核酸酶的核苷酸序列可操作地连接至多个控制元件，其允许在原核和真核细胞两者中表达编码C2C9核酸酶的核苷酸序列。在一些实施方案中，所述编码C2C9核酸酶的多核苷酸序列可操作地连接至用于在细胞或体外环境中表达的合适的核定位信号。
本发明中，所述编码C2C9核酸酶的多核苷酸可以通过人工合成方式进行合成，例如通过化学方法合成，从而使得能够容易地对其进行多种修饰。所述修饰可以采用本领域公知的任何修饰方式。在一些实施方案中，所述编码C2C9核酸酶的多核苷酸包含一种或多种修饰，从而使得能够容易地并入许多修饰，例如增强转录活性，改变酶活性，提高其翻译或稳定性(例如增加其对蛋白水解、降解的抗性)或特异性，改变溶解性，改变递送，减少宿主细胞中的先天免疫应答。所述修饰可以采用本领域公知的任何修饰方式。在一些实施方式中，通过修饰引入细胞中的编码C2C9核酸酶的DNA或RNA，以编辑任何一个或多个基因组的基因座。在一些实施方式中，编码C2C9核酸酶的核酸序列是修饰的核酸，例如密码子优化的。所述修饰可以是单一修饰，或组合修饰。
本发明中，包含编码C2C9核酸酶的多核苷酸的核酸可以是核酸模拟物。例如具有优良杂交性质的多核苷酸模拟物肽核酸等。
本发明中，所述C2C9核酸酶，或编码C2C9核酸酶的多核苷酸适用于任何生物或体外环境，包括但不限于是细菌、古细菌、真菌、原生生物、植物或动物。相应地，适用的靶细胞包括但不限于真核细胞和原核细胞，例如是细菌细胞、古细菌细胞、真菌细胞、原生生物细胞、植物细胞或动物细胞；所述真核细胞包含哺乳动物细胞和植物细胞，所述原核细胞包括大肠杆菌和肺炎克雷伯氏菌。适用的靶细胞可以是任何类型的细胞，包括干细胞，体细胞等。所述细胞可以是体内的或离体的。在某些实施方式中，所述C2C9核酸酶或编码该C2C9核酸酶的核酸配制在脂质体或脂质纳米颗粒中。
本发明还提供了所述C2C9核酸酶在构建CRISPR/C2C9基因编辑系统中的应用。
本发明还提供了用于在体内、离体细胞或体外环境中的合适的PAM序列，所述PAM为AAN和/或GAN。
本发明还提供了用于在原核、真核和体外环境中的合适PAM序列和合适的向导。本发明提供的引导RNA(gRNA)，其将核酸酶如C2C9核酸酶导向靶基因内的特定靶序列。
在一些实施方式中，所述gRNA包括：
与靶基因中的靶序列互补的核苷酸序列的第一片段(也称“基因靶向序列”或“基因靶向片段”)；以及，
与C2C9核酸酶相互作用的第二片段(也称“蛋白结合序列”或“蛋白结合片段”)。
在一些实施方式中，所述gRNA包含重复序列间隔区阵列，其中所述间隔区包含与基因中靶序列互补的核酸序列。
在一些实施方式中，所述gRNA包括：
i.能够与靶序列杂交的基因靶向区段(如DNA-靶向区段)，
ii.tracr配对物序列，和
iii.tracr RNA序列；
所述引导RNA为一条链，其由所述DNA-靶向区段(i)与tracr配对物序列(ii)和tracr RNA序列(iii)依次连接形成；或，所述引导RNA包括两条链，其中一条链由所述基因靶向区段(i)与tracr配对物序列(ii)连接形成，另一条链为所述tracrRNA序列(iii)。
其中，所述的基因靶向区段(i)较佳地为PAM序列之后长度为20bp的核酸片段所对应的RNA序列。
其中，所述tracr配对物序列(ii)与所述tracrRNA序列(iii)杂交，并形成茎-环结构。
其中，所述tracr配对物序列(ii)与所述tracrRNA序列(iii)能够连接在一起，形成单条的引导RNA骨架序列。
其中，与靶序列杂交的基因靶向区段(i)和tracr配对物序列(ii)连接得到的RNA序列(crRNA)，与所述tracrRNA序列(iii)，作为两条单独的RNA序列，在同时存在情况下可以介导C2C9核酸内切酶活性。或引导RNA骨架序列和与靶序列杂交的DNA-靶向区段(i)连接后得到的针对靶序列的完整引导RNA表达构建体，同样可以介导C2C9核酸内切酶活性。
gRNA的基因靶向区段包含与靶基因中的序列互补的核苷酸序列，该基因靶向序列通过杂交(即碱基配对)以序列特异性方式与靶基因相互作用。可以通过例如基因工程的方式修饰gRNA的基因靶向序列，以使得gRNA与靶基因内的任何所需序列杂交。gRNA通过上述基因靶向序列将结合的多肽引导至靶基因内的特定核苷酸序列。
所述茎-环结构形成与C2C9核酸酶相互作用的蛋白结合结构。在一些实施方式中，所述gRNA的蛋白结合结构包含4个茎环结构，tracr配对物序列(ii)通常与tracr RNA序列(iii)之间通过碱基互补进行配对。可以通过对gRNA的碱基序列进行改造来提高C2C9核酸酶的活性或者减少非特异性识别。
在一些实施方式中，靶基因是DNA序列。在一些实施方式中，靶基因是RNA序列。
在一些实施方式中，所述gRNA为sgRNA。在一优选实施方式中，所述的gRNA的序列如SEQ ID NO.123所示。
在一些实施方式中，靶基因的靶向序列可以具有12-40个核苷酸的长度，例如可以是13-20、18-25、22-32、26-37、30-38、32-40个核苷酸的长度。引导RNA的靶向区段(i)与靶基因的靶序列之间的互补性百分比可以为至少50％(例如，至少55％，至少60％，至少65％，至少70％，至少75％，至少80％，至少85％，至少90％，至少95％，至少97％，至少98％，至少99％或100％)。
在一些实施方式中，所述gRNA还包含转录终止子。
本发明还提供了修饰型gRNA，其可以通过修饰用于实现与靶基因内的任何所需序列杂交；或者，通过对gRNA修饰来改变gRNA本身的特性，如通过修饰增强gRNA的稳定性，包括但不限于通过增加其对细胞中存在的核糖核酸酶(RNase)降解的抗性，从而延长其在细胞中的半衰期；或者，其可以通过修饰用于增强包含gRNA和核酸内切酶(例如C2C9核酸酶)的CRISPR-C2C9基因组编辑复合体的形成或其稳定性；或者，其可以通过修饰用于增强基因组编辑复合体的特异性；或者，其可以通过修饰用于增强基因组编辑复合体与基因组中靶序列之间相互作用的起始位点，稳定性或动力学；或者，其可以通过修饰用于降低引入细胞中的RNA引发先天免疫反应的可能性或程度等。本发明中，可以通过对gRNA修饰来改变CRISPR-C2C9系统(如下文中所述)的多种特性，如增强CRISPR-C2C9基因组编辑复合物的形成、中靶活性、特异性、稳定性或动力学特性。可以采用本领域已知的修饰方式对RNA进行修饰，包括但不限于在嘧啶的核糖、碱基残基或RNA的3'端的反向碱基上的2'-氟、2'-氨基修饰等。本发明中，可以采用任意一种修饰或多种修饰组合对gRNA进行修饰。在一些实施方式中，通过修饰引入细胞中的sgRNA，以编辑任何一个或多个基因组的基因座。
本发明提供了包含编码gRNA的核苷酸序列的核酸。在某些实施方式中，编码gRNA的核酸是表达载体，例如重组表达载体。可以使用任何合适的表达载体，只要它与宿主细胞相容即可，包括但不限于，病毒载体(例如基于痘苗病毒的病毒载体；脊髓灰质炎病毒；腺病毒；腺相关病毒；SV40；单纯疱疹病毒；人免疫缺陷病毒；反转录病毒载体(例如鼠白血病病毒，脾脏坏死病毒，以及衍生自反转录病毒的载体，例如劳斯肉瘤病毒，Harvey肉瘤病毒，禽类白血病病毒，慢病毒，人免疫缺陷病毒，骨髓增生肉瘤病毒和乳腺肿瘤病毒)等。
在某些实施方式中，在相同细胞中同时使用多种gRNA以同时调节相同靶基因或不同靶基因上的不同位置的转录。当多个gRNA同时使用时，它们可以存在于相同的表达载体上或不同的载体上，也可以同时表达；当存在于相同的载体上时，它们可以在相同的控制元件下进行表达。
在某些实施方式中，编码gRNA的核苷酸序列可操作地连接至控制元件，例如转录控制元件，例如启动子。在某些实施方式中，编码gRNA的核苷酸序列可操作地连接至诱导型启动子。在某些实施方式中，编码gRNA的核苷酸序列可操作地连接至组成型启动子。转录控制元件可以在真核细胞，例如哺乳动物细胞；或原核细胞(例如细菌或古细菌细胞)中起作用。在某些实施方式中，编码gRNA的核苷酸序列可操作地连接至多个控制元件，其允许在原核和真核细胞两者中表达编码gRNA的核苷酸序列。
本发明中，所述gRNA可以通过人工合成方式进行合成，例如通过化学方法合成，从而使得能够容易地对其进行多种修饰。所述修饰可以采用本领域公知的任何修饰方式，例如，使用polyA尾巴，添加5'帽类似物，5'或3'非翻译区(UTR)，5'或3'端包括硫代磷酸化2’-O-甲基核苷酸或用磷酸酶处理以去除5'末端磷酸酯等。
在一些实施方案中，所述编码gRNA的核苷酸序列包含一种或多种修饰，其可用于例如增强活性，稳定性或特异性，改变递送，减少宿主细胞中的先天免疫应答或用于其他增强。
在一些实施方式中，将增强编码gRNA的核苷酸序列的活性，细胞分布或细胞摄取的一个或多个靶向部分或缀合物化学连接至gRNA。所述靶向部分或缀合物可包括与功能基团共价结合的缀合物基团；缀合物基团包括报告分子，多胺，聚乙二醇。在一些实施方式中，将增强药效学性质的基团连接至gRNA，增强药效学性质的基团包括改善摄取，增强对降解的抗性和/或增强与靶核酸的序列特异性杂交的基团。
本发明中，包含编码gRNA的多核苷酸的核酸可以是核酸模拟物。例如具有优良杂交性质的多核苷酸模拟物肽核酸等。
本发明中，所述gRNA，或编码gRNA的多核苷酸适用任何生物或体外环境，包括但不限于是细菌、古细菌、真菌、原生生物、植物或动物。相应地，适用的靶细胞包括但不限于细菌细胞、古细菌细胞、真菌细胞、原生生物细胞、植物细胞或动物细胞。适用的靶细胞可以是任何类型的细胞，包括干细胞，体细胞等。
本发明还提供了一种重组表达载体，其包含(i)编码gRNA的核苷酸序列，和(ii)编码C2C9核酸酶的核苷酸序列。在一实施方式中，靶基因内调节的转录的位点由所述gRNA确定。
本发明还提供了基于C2C9核酸酶的CRISPR-C2C9系统，其包含：(a)如上文所述的C2C9核酸酶或如上文所述的包含编码所述C2C9核酸酶的多核苷酸的核酸；和/或，(b)如上文所述的一种或多种gRNA或编码所述gRNA的核酸。其中所述gRNA能够将所述C2C9核酸酶指导至靶序列。
在一些实施方式中，C2C9核酸酶直接作为蛋白提供；例如可以使用原生质球转化用外源蛋白和/或核酸转化真菌的方式。可以通过任何合适的方法将C2C9核酸酶引入细胞，如注射方式等。
本发明所述系统中，C2C9核酸酶和gRNA可以在宿主细胞中形成复合物，识别靶向基因(如靶向DNA)序列上的PAM序列；所述CRISPR/C2C9基因编辑系统的靶向序列为PAM序列之后长度为20bp的核酸片段(如DNA片段)。在一实施方式中，所述复合物可以选择性地调节宿主细胞中靶DNA的转录。CRISPR/C2C9基因编辑系统能够切割靶向DNA的双链，造成DNA断裂。在靶向链(和gRNA互补配对的DNA单链，也称为Targeting strand或TS链)的主要切割位点为PAM位点3’端下游的21-22nt处，而在非靶向链(不和gRNA互补配对的DNA单链，也称为Non-targeting strand或NTS链)的主要切割位点为PAM位点3’端下游的15-16nt处。
在一实施方式中，所述系统包含gRNA和C2C9核酸酶复合物。
在一实施方式中，所述系统包含重组表达载体。在一实施方式中，所述系统包含重组表达载体，所述重组表达载体包含(i)编码gRNA的核苷酸序列，其中所述gRNA包含：(a)包含与靶DNA中的序列互补的核苷酸序列的第一区段；和(b)与C2C9核酸酶相互作用的第二区段；和(ii)编码C2C9核酸酶的核苷酸序列，其中所述C2C9核酸酶包含：(a)与所述gRNA相互作用的RNA-结合部分；和(b)调节靶DNA内转录的活性部分，其中靶DNA内调节的转录的位点由所述gRNA确定。
在某些实施方式中，所述系统包含如SEQ ID NO:3所示的C2C9核酸酶(或由其组成)。在某些实施方式中，所述系统包含编码C2C9核酸酶的核酸，所述核酸与SEQ ID NO:121或SEQ ID NO:122具有至少约90％(诸如至少约91％、92％、93％、94％、95％、96％、97％、98％、99％)序列同源性。在某些实施方式中，所述系统包含编码C2C9核酸酶的核酸，所述核酸包含SEQ ID NO:121或SEQ ID NO:122的序列(或由其组成)。在某些实施方式中，所述系统中的gRNA是单个gRNA(sgRNA)。在某些实施方式中，所述系统进一步包括一种或多种另外的gRNA或编码一种或多种另外的gRNA的核酸。
在某些实施方式中，所述系统含有用于在细胞或体外环境中表达的合适启动子和/或合适的核定位信号；编码所述C2C9核酸酶的多核苷酸序列可操作地连接至(i)用于在细胞或体外环境中表达的合适启动子；和/或(ii)合适的核定位信号。
在某些实施方式中，所述系统中，编码所述gRNA的多核苷酸序列可操作地连接至(i)用于在细胞或体外环境中表达的合适启动子；和/或(ii)合适的核定位信号。
在某些实施方式中，本发明提供了一种系统，其包含：(a)C2C9核酸酶，其包含SEQIDNO:3的氨基酸序列或其与SEQ ID NO:3有至少90％序列同源性的变体；和(b)gRNA，其中所述gRNA能够将所述C2C9核酸酶或其变体指导至靶多核苷酸序列。
在某些实施方式中，本发明提供了一种系统，其包含：(a)C2C9核酸酶或编码C2C9核酸酶的核酸，所述C2C9核酸酶包含SEQ IDNO:3的氨基酸序列或其与SEQ ID NO:3具有至少90％序列同源性的变体；所述编码C2C9核酸酶的核酸包含SEQ ID NO:121或SEQ ID NO:122中的任一个的多核苷酸序列或其与SEQ ID NO:121或SEQ ID NO:122中的任一个的多核苷酸序列具有至少90％序列同源性的变体；和(b)gRNA或编码gRNA的核酸，其中所述gRNA能够将所述C2C9核酸酶或其变体指导至靶多核苷酸序列。在某些实施方式中，所述gRNA是sgRNA。
本发明所述系统可以包含一个gRNA或同时包含多个gRNA。在一实施方式中，所述系统同时包含多个gRNA以同时修饰相同靶标DNA或不同靶标DNA上的不同位置。在一实施方案中，两个或更多个引导RNA靶向相同的基因或转录本或基因座。在一实施方案中，两个或更多个引导RNA靶向不同的不相关基因座。在一些实施方案中，两个或更多个引导RNA靶向不同但相关的基因座。
本发明所述系统中的各组成部分可通过载体方式来运送。例如，对于多核苷酸，可以采用的方法包括但不限于纳米颗粒、脂质体、核糖核蛋白、小分子RNA-缀合物、嵌合体和RNA-融合蛋白复合物等。
在一些实施方案中，所述C2C9或gRNA可以构建在载体上，编码C2C9和/或gRNA的核苷酸序列可操作地连接至控制元件，例如转录控制元件，如启动子。转录控制元件可以在真核细胞(例如哺乳动物细胞)或原核细胞(例如细菌或古细菌细胞)中起作用。在一些实施方案中，编码gRNA和/或C2C9核酸酶的核苷酸序列可操作地连接至多个控制元件，其允许在两个原核生物中表达编码引导RNA和/或定点修饰多肽的核苷酸序列。在一些实施方案中，编码gRNA和/或C2C9核酸酶的核苷酸序列构建在同一载体上。在一些实施方案中，编码gRNA和/或C2C9核酸酶的核苷酸序列构建在不同载体上。在某些实施方式中，编码gRNA和/或C2C9核酸酶的核苷酸序列可操作地连接至组成型启动子。在某些实施方式中，编码gRNA和/或C2C9核酸酶的核苷酸序列可操作地连接至诱导型启动子。
本发明所述的系统还可以进一步包括一个或多个供体模板。在某些实施方式中，所述供体模板包含用于插入靶基因的供体序列。在某些实施方式中，所述供体模板包含供体盒，该供体盒在一侧或两侧侧翼有gRNA靶位点。在某些实施方式中，所述供体模板的供体盒的两侧侧翼有gRNA靶位点，其中该供体模板的gRNA靶位点是该系统中gRNA的基因组gRNA靶位点的反向互补序列。供体序列通常与其置换的基因组序列不同，即，供体序列通常含有相对于基因组序列的碱基改变、插入、缺失、倒位或重排，且存在足够的同源性以支持同源性指导的修复。供体序列可以是单链DNA、单链RNA、双链DNA或双链RNA。在一些实施方式中，所述供体序列是外源序列。在一些实施方式中，所述供体序列为供体DNA序列(包括供体单链或双链DNA)，其中靶DNA序列通过同源性指导的修复进行编辑。在某些实施方式中，供体模板与系统中的gRNA物理连接。在某些实施方式中，完整或部分或完全缺陷的C2C9核酸酶、或gRNA与供体模板连接，以通过一种或多种gRNA分子指导到一个或多个特定的靶基因(如靶DNA)位点，从而促进外源DNA序列的同源重组。
本发明所述系统中，还可以进一步包含二聚体FOK1核酸酶，完整或部分或完全缺陷的C2C9核酸酶或gRNA连接至二聚体FOK1核酸酶，以在通过一种或多种gRNA分子指导到一个或多个特定DNA靶位点时指导内切核酸酶切割。
本发明所述系统可以在细胞中的多个位置处编辑或修饰DNA，以用于基因治疗，包括但不限于用于疾病的基因治疗，用于生物学研究，用于农作物抗性改进或提高产量等。
本发明中，所述系统适用于任何生物或体外环境，包括但不限于是细菌、古细菌、真菌、原生生物、植物或动物。相应地，适用的靶细胞包括但不限于细菌细胞、古细菌细胞、真菌细胞、原生生物细胞、植物细胞或动物细胞。适用的靶细胞可以是任何类型的细胞，包括干细胞，体细胞等。所述细胞可以是体内的或离体的。
本发明还提供了一种组合物，其包含如上所述的C2C9核酸酶或编码其的多核苷酸、gRNA或编码其的多核苷酸、重组表达载体、系统中的一种或几种，还可以包括可接受的载体、介质等。所述可接受的载体、介质例如无菌水或生理盐水、稳定剂、赋形剂、抗氧化剂(抗坏血酸等)、缓冲剂(磷酸、枸橼酸、其它的有机酸等)、防腐剂、表面活性剂(PEG、Tween等)、螯合剂(EDTA等)、粘合剂等。而且，也可含有其它低分子量的多肽；血清白蛋白、明胶或免疫球蛋白等蛋白质；甘氨酸、谷酰胺、天冬酰胺、精氨酸和赖氨酸等氨基酸；多糖和单糖等糖类或碳水化物；甘露糖醇或山梨糖醇等糖醇。当制备用于注射的水溶液时，例如生理盐水、含有葡萄糖或其它的辅助药物的等渗溶液，如D-山梨糖醇、D-甘露糖、D-甘露糖醇、氯化钠，可并用适当的增溶剂例如醇(乙醇等)、多元醇(丙二醇，PEG等)、非离子表面活性剂(吐温80，HCO-50)等。在一些实施方式中，组合物包含gRNA和用于稳定核酸的缓冲液。
本发明还提供了一种试剂盒，其包括如上所述的系统或组合物。所述试剂盒还可以进一步包括一种或多种例如选自：稀释缓冲液；洗涤缓冲液；对照试剂等的额外试剂。在一些实施方式中，所述试剂盒包括(a)根据上文所述的C2C9核酸酶或编码C2C9核酸酶的核酸；和(b)gRNA或编码所述gRNA的核酸，其中所述gRNA能够将所述C2C9核酸酶或其变体指导至靶多核苷酸序列。在某些实施方式中，所述试剂盒进一步含有包含异源多核苷酸序列的供体模板，其中所述异源多核苷酸序列能够被插入所述靶多核苷酸序列中。
本发明提供了如上所述的C2C9核酸酶或编码其的多核苷酸、gRNA或编码其的多核苷酸、重组表达载体、系统、组合物和试剂盒用于体内、离体细胞或无细胞系统中以下任一用途，包括但不限于：
切割靶基因；
操控靶基因的表达；
遗传修饰靶基因；
遗传修饰靶基因相关多肽；
用于在靶基因的任何所需位置处的有意和受控的损伤；
用于在靶基因的任何所需位置处的有意和受控的修复；
引入双链断裂以外的方式修饰靶基因(C2C9核酸酶具有酶活性，其以除引入双链断裂以外的方式修饰靶基因；所述酶活性可以是C2C9本身所具有的，或通过例如将具有酶活性的异源多肽融合到C2C9核酸酶形成嵌合C2C9核酸酶获得的，所述酶活性包括但不限于甲基转移酶活性、脱氨作用活性、歧化酶活性、烷基化活性、脱甲基酶活性、DNA修复活性、转座酶活性、重组酶活性、DNA损伤活性、脱嘌呤活性、氧化活性、嘧啶二聚体形成活性等)。
在一些实施方式中，所述靶基因为靶DNA。在一些实施方式中，所述靶基因为靶RNA。
本发明中，所述C2C9可以通过与其他酶成分或其他成分结合使用，以进一步开发C2C9核酸酶的各种潜在应用。作为非限制性的例子，例如通过融合失活的C2C9和碱基脱氨酶开发基于C2C9核酸酶的单碱基编辑系统；通过融合失活的C2C9和逆转录酶开发基于C2C9核酸酶的Prime编辑系统；通过融合失活的C2C9和转录激活因子，开发基于C2C9核酸酶的转录激活系统；通过融合失活的C2C9和核酸表观修饰酶，开发基于C2C9核酸酶的表观修饰系统；利用失活的C2C9，开发基于C2C9核酸酶的转录抑制系统。
本发明C2C9核酸酶或编码其的多核苷酸、gRNA或编码其的多核苷酸、重组表达载体、系统、组合物和试剂盒可以应用于研究领域，诊断领域，工业领域(例如微生物工程)，药物发现(例如高通量筛选)，靶标确认，影像学领域以及治疗领域等。应用于研究领域可以是，例如确定提高调控靶核酸的转录对例如发育、生长、代谢(如通过控制特定酶的水平来精确地控制和调节生物合成途径)、基因表达等的影响。
本发明中，所述C2C9核酸酶或编码其的多核苷酸、gRNA或编码其的多核苷酸、重组表达载体、系统、组合物和试剂盒等适用任何生物，包括但不限于是细菌、古细菌、真菌、原生生物、植物或动物。相应地，适用的靶细胞包括但不限于细菌细胞、古细菌细胞、真菌细胞、原生生物细胞、植物细胞或动物细胞(例如啮齿类动物细胞，人细胞，非人灵长类动物细胞)。适用的靶细胞可以是任何类型的细胞，包括干细胞，体细胞等。
当本发明所述C2C9核酸酶、gRNA、系统、组合物和方法等适用于真核细胞中时，可以例如用于哺乳动物细胞中。在一些实施方式中，所述细胞不是人胎儿细胞或不源自人胎儿细胞。在一些实施方式中，所述细胞不是人胚胎细胞或不源自人胚胎细胞。在一些实施方式中，本发明中不涉及人胎儿或人胚胎的破坏。在一些实施方式中，所述受试者或个体不是人胎儿或胚胎。
当本发明所述C2C9核酸酶或编码其的多核苷酸、gRNA或编码其的多核苷酸、重组表达载体、系统、组合物和试剂盒等适用于原核细胞中时，例如大肠杆菌中。在一些实施方式中，用于关闭细菌细胞中基因表达。在一些实施方式中，将编码合适的gRNA和/或合适的C2C9核酸酶的核酸导入靶细胞的染色体中，所述核酸在诱导型启动子的控制下翻译表达；其中，编码的gRNA和C2C9核酸酶两者形成复合物，在靶DNA感兴趣的位置进行切割。因此，在一些实施方式中，通过工程改造方式，细菌基因组中包含编码适当C2C9核酸酶的核酸序列和/或质粒上适当的gRNA，在诱导型启动子的控制下，通过诱导gRNA和C2C9核酸酶的表达控制任何靶向的基因的表达。在一些实施方式中，C2C9核酸酶具有酶活性，其以除引入双链断裂以外的方式修饰靶DNA；所述酶活性可以是C2C9本身所具有的，或通过例如将具有酶活性的异源多肽融合到C2C9核酸酶形成嵌合C2C9核酸酶获得的，所述酶活性包括但不限于甲基转移酶活性、脱氨作用活性、歧化酶活性、烷基化活性、脱甲基酶活性、DNA修复活性、转座酶活性、重组酶活性、DNA损伤活性、脱嘌呤活性、氧化活性、嘧啶二聚体形成活性等。本发明中，可通过将所期望的互补核酸序列基因工程改造到gRNA的基因靶向区段(如DNA-靶向区段)中而用于控制在靶基因中任何位置处的靶基因的遗传修饰。本发明中，可通过将所期望的互补核酸序列基因工程改造到C2C9而用于控制在靶基因中任何位置处的靶核酸的遗传修饰。在一些实施方式中，应用于在细菌靶DNA内任何所需位置处的DNA的有意和受控的损伤。在一些实施方式中，用于在细菌靶DNA内任何所需位置处的DNA的序列特异性和受控修复。
本发明还提供了在细胞中或在体内、离体细胞或无细胞系统中靶向、编辑、修饰或操纵靶基因(如靶DNA)的方法，包括：将如上所述的C2C9核酸酶或编码其的多核苷酸、gRNA或编码其的多核苷酸、重组表达载体、系统、组合物等引入试剂盒体内、离体细胞或无细胞系统中，对靶基因进行靶向、编辑、修饰或操纵。
在一实施方式中，所述方法包括如下：
(a)将所述C2C9核酸酶或编码C2C9核酸酶的核酸引入体内、离体细胞或无细胞系统中；和
(b)引入所述gRNA(sgRNA)或适合于原位产生这种sgRNA的核酸(例如，DNA)；和
(c)使细胞或靶基因与C2C9核酸酶或编码C2C9核酸酶的核酸、gRNA(sgRNA)或适合于原位产生这种sgRNA的核酸接触，以在所述靶基因中产生一个或多个切割、切口或编辑；其中所述C2C9核酸酶通过其加工的或未加工形式的gRNA被指导至所述靶基因。
在一些实施方式中，所述靶基因为靶DNA。在一些实施方式中，靶DNA可以是未与DNA相关蛋白结合的体外裸DNA。在一些实施方式中，靶DNA是体外细胞中的染色体DNA。在一些实施方式中，所述靶基因为靶RNA。在一些实施方式中，将靶DNA与包含所述C2C9核酸酶和gRNA的靶向复合物接触，gRNA通过包含与靶DNA互补的核苷酸序列，为靶向复合物提供靶特异性；C2C9核酸酶提供位点特异性活性。在一些实施方式中，靶向复合物修饰靶DNA，从而导致例如DNA切割、DNA甲基化作用、DNA损伤、DNA修复等。在一些实施方式中，靶向复合物修饰与靶DNA相关多肽(例如，组蛋白、DNA-结合蛋白等)，从而导致例如靶DNA相关多肽-组蛋白的甲基化、组蛋白乙酰化、组蛋白泛素化等。
本发明所述方法中，当应用于体内时，将C2C9核酸酶或编码其的多核苷酸、gRNA或编码其的多核苷酸、重组表达载体、系统、组合物和/或供体多核苷酸直接施用于个体。
本发明所述方法中，可以通过公知的方法将C2C9核酸酶或包含编码C2C9核酸酶的多肽的核苷酸序列的核酸引入细胞。同样地，可以通过公知的方法将gRNA或包含编码gRNA的核苷酸序列的核酸引入细胞。公知的方法包括，DEAE-葡聚糖介导的转染、脂质体介导的转染、病毒或噬菌体感染、脂质转染法、转染、接合、原生质体融合、聚乙烯亚胺介导的转染、电穿孔、磷酸钙沉淀、基因枪、磷酸钙沉淀、显微注射、纳米颗粒介导的核酸递送等。例如通过电穿孔、氯化钙转染、显微注射和脂质转染法等递送质粒。对于病毒载体递送，使细胞与包含编码gRNA和/或C2C9核酸酶和/或嵌合C2C9核酸酶的核酸和/或供体多核苷酸的病毒颗粒接触。
在一些实施方式中，本发明所述方法中，核酸酶切割细胞中的靶DNA以产生双链断裂，然后由细胞通常以下列方式进行修复：非同源末端连接(NHEJ)和同源性指导的修复。
在非同源末端连接中，通过将断裂末端直接相互连接来修复双链断裂。在所述过程中，可以在切割位点插入或缺失少数碱基对。因此，C2C9核酸酶对DNA的切割可用于通过切割靶DNA序列并允许细胞在没有外源提供的供体多核苷酸的情况下修复所述序列来从靶DNA序列删除核酸材料。因此，所述方法可用于敲除基因或将遗传物质敲入靶DNA中选择的基因座。
在同源性指导的修复中，与切割的靶DNA序列具有同源性的供体多核苷酸被用于修复切割的靶DNA序列的模板，从而导致遗传信息从供体多核苷酸转移至靶DNA。因此，可以将新核酸材料插入/复制到位点中。在一些实施方式中，靶DNA与供体多核苷酸接触。在一些实施方式中，将供体多核苷酸引入细胞中。由于NHEJ和/或同源性指导的修复对靶DNA的修饰导致例如基因矫正、基因置换、转基因插入、核苷酸缺失、核苷酸插入、基因破坏、基因突变、序列置换等。
在一些实施方式中，包含靶基因的细胞是体外的。在一些实施方式中，包含靶基因的细胞是体内的。包含编码gRNA和/或C2C9核酸酶的核苷酸序列的合适核酸包括表达载体，其中包含编码gRNA和/或C2C9核酸酶的核苷酸序列的表达载体是重组表达载体。在一些实施方式中，所述方法通过相同或不同重组载体将C2C9核酸酶和一种或多种gRNA(gRNA)引入细胞中，使细胞与包含编码gRNA和/或C2C9核酸酶的多核苷酸的载体接触，以使载体被细胞吸收。
本发明上述方法还可以用于在细胞中的多个位置处编辑或修饰DNA。
本发明上述方法还可以用于调控靶DNA的转录。
在一些实施方式中，本发明提供的方法，包括将完整或部分或完全缺陷的C2C9核酸酶或gRNA部分连接至二聚体FOK1核酸酶，以在通过一种或多种crRNA分子指导到一个或多个特定DNA靶位点时指导内切核酸酶切割。在其他一些实施方式中，本发明提供的方法，将与二聚体FOK1核酸酶连接的C2C9核酸酶与进入细胞的包含两种或更多种作为RNA或编码为DNA并在一个启动子控制下的gRNA一起引入细胞，每种gRNA包含重复序列间隔区阵列，其中所述间隔区包含与DNA中靶序列互补的核酸序列，且所述重复序列包含茎-环结构。在一些实施方式中，本发明提供的方法，包括将完整或部分或完全缺陷的C2C9核酸酶gRNA与供体单链或双链DNA供体模板连接，以通过一种或多种gRNA分子指导到一个或多个特定的DNA靶位点，从而促进外源DNA序列的同源重组。
本发明还提供了用于靶基因-相关多肽的方法，所述方法包括使靶基因与靶向复合物接触，所述靶复合物包括上文所述的C2C9核酸酶或编码C2C9核酸酶的核酸、以及gRNA或适合于原位产生这种sgRNA的核酸接触。
在一些实施方式中，靶基因-相关多肽为组蛋白、DNA-结合蛋白等。在一些实施方式中，靶向复合物修饰与靶DNA相关多肽，从而导致例如靶DNA相关多肽例如组蛋白的甲基化、组蛋白乙酰化、组蛋白泛素化等。
在一些实施方式中，所述靶基因为靶DNA。在一些实施方式中，靶DNA是体外细胞中的染色体DNA。在一些实施方式中，靶DNA是体内细胞中的染色体DNA等。在一些实施方式中，所述靶基因为靶RNA。在一些实施方式中，将靶DNA与包含所述C2C9核酸酶和gRNA的靶向复合物接触，gRNA和C2C9核酸酶形成复合物，gRNA通过包含与靶DNA互补的核苷酸序列，为靶向复合物提供靶特异性；C2C9核酸酶提供位点特异性活性。在某些实施方式中，基于C2C9核酸酶的CRISPR-C2C9系统中使用修饰的多核苷酸，可以修饰引入细胞中的编码C2C9核酸酶的DNA或RNA和/或gRNA，以编辑任何一个或多个基因组的基因座。
本发明还提供了一种细胞，包括已用上述C2C9核酸酶或编码其的多核苷酸、gRNA或编码其的多核苷酸、重组表达载体、系统、组合物对宿主细胞进行了遗传修饰。
本发明中，gRNA和/或C2C9核酸酶和/或重组表达载体和/或供体多核苷酸的有效剂量对于本领域技术人员而言是常规的。可以根据不同的施用途径以及治疗的病症的特性来确定。
C2C9核酸酶是申请人发现的一种新型适用于CRISPR基因编辑系统的核酸酶，在对应的引导RNA导向下能够精确定位到目标基因，进行基因组DNA切割，实现基因组DNA双链断裂。C2C9核酸酶变体可以具有不同的或有利特征。这些C2C9核酸酶及其变体提供了以前不存在的基因组编辑的其它机会。C2C9核酸酶及其变体可以具有不同的活性，取决使用它们的系统。尽管C2C9在分子量上只有Cas9和Cas12a的一半或更小，但C2C9具有接近或类似Cas9和Cas12a的基因组切割效率，能够在原核细菌和真核细胞中实现高效的基因组编辑，因而在生物技术和基因治疗等领域具有极大的应用前景。
本发明提供的C2C9核酸酶相对于已报道的CRISPR-Cas内切核酸酶可以表现出有利的特性，例如在原核、真核和/或体外环境中的更高活性。在一些实施方式中，C2C9组合了以下一种或多种：小尺寸，高编辑活性，和仅需要短PAM序列。本发明关于RNA-指导的核酸酶的小尺寸是指长度不大于约800个氨基酸的核酸酶。
本发明中，所述细菌或原核细菌可以是大肠杆菌、肺炎克雷伯氏菌、卵形拟杆菌、空肠弯曲菌、腐生葡萄球菌、粪肠球菌、多形拟杆菌、普通拟杆菌、单形拟杆菌、干酪乳杆菌、脆弱拟杆菌、鲁氏不动杆菌、具核梭杆菌、乔氏拟杆菌、拟南芥拟杆菌、鼠李糖乳杆菌、马赛拟杆菌、粪副拟杆菌、死亡梭杆菌和短双歧杆菌等。
本发明中，所述真核细胞包括但不限于是哺乳动物细胞、真菌等真核生物细胞。所述真菌包括酵母、曲霉，例如可以是酿酒酵母、多形汉逊酵母、毕赤酵母、脆壁克鲁维氏酵母、乳酸克鲁维氏酵母，以及栗酒裂殖酵母、白假丝酵母、杜氏假丝酵母、光滑假丝酵母、季也蒙假丝酵母、乳酒假丝酵母、克鲁斯假丝酵母、葡萄牙假丝酵母、梅林假丝酵母、嗜油假丝酵母、近平滑假丝酵母、热带假丝酵母和产朊假丝酵母、烟曲霉、黄曲霉、黑曲霉、棒曲霉、灰绿曲霉群、构巢曲霉、米曲霉、土曲霉、焦曲霉和杂色曲霉等。
在本发明的实施例中，公开了一种基于极小型CRISPR/C2C9核酸酶的新型基因组编辑方法。该发明显示，通过引导RNA的导向和定位功能，C2C9能够精确切割基因组DNA，实现基因组DNA双链断裂。利用宿主细胞自身或外源的修复机制，该系统能够高效和精确地实现活细胞内基因编辑。
下面将结合实施例对本发明的实施方式进行清楚、完整的描述，显然，所描述的实施例仅用于说明本发明的一部分实施例，而不应视为限制本发明的范围。实施例中未注明具体条件者，按照常规条件或制造商建议的条件进行。所用试剂或仪器未注明生产厂商者，视为可以通过市售购买获得的常规产品。
各实施例中使用的生物材料来源如下：
肺炎克雷伯菌NCTC9633菌株(购自美国ATCC公司，货号：13883)；感受态大肠杆菌DH5α菌株(购自康为世纪生物科技有限公司，货号：CW0808S)。各实施例中使用的LB液体培养基购自生工生物工程(上海)股份有限公司，货号：A507002-0250；LB固体培养基购自生工生物工程(上海)股份有限公司，货号：A507003-0250；阿泊拉霉素购自美国BioVision公司，货号：B1521-1G；卡那霉素购自上海阿拉丁生化科技股份有限公司，货号：K103024-5g。
实施例一：AcC2C9的前间区序列邻近基序(PAM)鉴定质粒p15a-AcC2C9Control和p15a-AcC2C9PAM的构建
(1.1)p15a-AcC2C9Control质粒序列为SEQ ID NO:124.其具体构建方法如下：
在生工生物工程(上海)股份有限公司全基因合成AcC2C9核酸酶及其对应的引导RNA表达盒的DNA序列(其序列为SEQ ID NO:125)以及p15a质粒骨架(其序列为SEQ ID NO:126)。使用Gibson assembly技术将这两个DNA片段组装为p15a-AcC2C9Control质粒。转化大肠杆菌DH5α商品感受态细胞，涂布在带有75μg/mL阿泊拉霉素的LB培养基平板上筛选。挑取单克隆后扩大培养，提取质粒，测序鉴定质粒序列后，获得p15a-AcC2C9Control质粒。
(1.2)p15a-AcC2C9PAM质粒序列为SEQ ID NO:127，其具体构建方法如下：
在生工生物工程(上海)股份有限公司合成以下两条引物：
PAMprimerF：5’-atcgTGTCCTCTTCCTCTTTAGCG-3’(SEQ ID NO:128)
PAMprimerR：5’-ggccCGCTAAAGAGGAAGAGGACA-3’(SEQ ID NO:129)
将上述两条引物进行退火，具体反应体系如下：5μl 10×T4 DNA ligase Buffer(NEB公司)，10μLPAM primerF(10μM)，10μLPAM primerR(10μM)，25μL ddH2O。在95℃加热5min，然后在1～2小时内缓慢地降低到室温，使两条单链引物通过碱基配对形成双链DNA。然后用ddH2O将得到的产物稀释20倍。
将上述得到的双链DNA通过Golden Gate技术插入p15a-AcC2C9Control质粒的BsaI位点，具体反应体系为：1μL 10×T4 DNA ligase Buffer，1μL上述20倍稀释的磷酸化双链DNA，20fmolp15a-AcC2C9Control质粒，0.5μL T4 DNA ligase(400units/μL)，0.5μLBsaI-HF(20units/μL)，最后加入适量ddH2O至总体积为10μL。该反应中所用Buffer和酶均由NEB公司生产。在PCR仪中进行反应，循环如下：37℃2min；16℃5min，共25个循环；然后80℃15min。
将该10μL反应产物转化到大肠杆菌感受态DH5α菌株中，并平铺在含有75μg/mL阿泊拉霉素的LB固体培养平板上。待转化液被固体培养平板吸收后于37℃培养箱倒置培养过夜。将培养基上长出的转化菌株转接保存，同时抽提质粒并送到生工生物工程(上海)股份有限公司进行测序验证，最终得到p15a-AcC2C9PAM质粒。
实施例二 用于PAM鉴定的底物质粒pUC19-6N-library的构建
pUC19-6N-library质粒序列为SEQ ID NO:130.其具体构建方法如下：
以商品pUC19质粒为模板，使用带有6N随机序列的引物6N-F(其序列为SEQ ID NO:131)和普通引物6N-R(其序列为SEQ ID NO:132)进行环式聚合酶延伸克隆(circularpolymerase extension cloning)。PCR产物经DpnI消化处理后，转化大肠杆菌DH5α商品感受态细胞，涂布带有羧苄青霉素的LBA平板，用涂布器刮取收集大于10万个单克隆，混合后提取质粒，获得PAM鉴定底物质粒pUC19-6N-library。
实施例三AcC2C9的PAM鉴定
将实施例一中构建的p15a-AcC2C9Control质粒转化到大肠杆菌DH5α商品感受态细胞中，然后涂布在含有75μg/mL阿泊拉霉素的LB固体平板上。次日挑取单克隆于100mL的新鲜LB液体培养基中进行培养，待OD600＝0.5时收集细菌后使用冰上预冷的灭菌10％v/v甘油洗涤菌体两次，最终用1mL灭菌10％v/v甘油溶液重悬细菌，获得带有p15a-AcC2C9Control质粒的DH5α感受态细胞。
制备带有实施例一中构建的p15a-AcC2C9PAM质粒的DH5α感受态细胞，其制备过程与上述带有p15a-AcC2C9Control质粒的DH5α感受态细胞制备过程相同。
取100ng实施例二中构建的pUC19-6N-library质粒，电转到带有p15a-AcC2C9Control质粒的DH5α感受态细胞中，涂布带有50μg/mL羧苄青霉素和75μg/mL阿泊拉霉素的LB固体平板上。分别用涂布器刮取收集大于10万个单克隆，混合后提取质粒，获得对照文库(control library)
取100ng实施例二中构建的pUC19-6N-library质粒，电转到带有p15a-AcC2C9PAM质粒的DH5α感受态细胞中，制备AcC2C9的PAM缺失文库(PAM depletion library)。其制备过程与对照文库的步骤相同。
分别以control library和PAM depletion library为模板，使用引物PAM-1F(其序列为SEQ ID NO:133)和PAM-1R(其序列为SEQ ID NO:134)，进行第一轮PCR，电泳检测产物，再取1μL第一轮PCR产物为模板，使用引物PAM-2F(其序列为SEQ ID NO:135)和PAM-2R(其序列为SEQ ID NO:136)进行第二轮PCR，电泳检测产物。分别使用VAHTS DNA CleanBeads磁珠(诺维赞生物科技股份有限公司)纯化产物。分别使用Illumina Hiseq2500平台进行高通量测序，各获得1Gb原始数据，提取6N随机序列信息，以PAM depletion library中的序列频率比较control library中的序列频率，建立weblogo，从而获得AcC2C9偏好的PAM信息。
图1为AcC2C9的PAM的鉴定流程及结果图。结果显示：AcC2C9能有效识别5’-AAN类型的PAM序列以及少量5’-GAN序列(其中N为简并碱基，代表A、T、C或G任意一种碱基)。其中，横坐标-1、-2、-3、-4、-5、-6分别代表NTS链(非靶向链)上位于靶向-DNA区段序列5’端上游的第1、第2、第3、第4、第5、第6个DNA碱基；其上的字母代表AcC2C9核酸酶对DNA序列的偏好性，字母所占的比重越大，代表AcC2C9对该碱基的偏好性越强。
实施例四 构建用于肺炎克雷伯菌基因组编辑的p15a-AcC2C9和pSGKP-AcC2C9质粒
p15a-AcC2C9质粒序列为SEQ ID NO:137，其具体构建方法如下：
以实施例一中的p15a-AcC2C9Control质粒为模板，分别扩增AcC2C9核酸酶基因和p15a质粒骨架。
扩增AcC2C9核酸酶基因5’引物序列AcC2C9F:
tcatctgtgcatatagctatactgatttcgtcagactcaca(SEQ ID NO：138)
扩增AcC2C9核酸酶基因3’引物序列ACC2C9R:
cgccaaccagccaCCTTATTGACCTGCACCGCTATG(SEQ ID NO：139)
扩增p15a质粒骨架5’引物序列p15aF:
CAGGTCAATAAGGtggctggttggcgtactgtt(SEQ ID NO：140)
扩增p15a质粒骨架3’引物序列p15aR:
atcagtatagctatatgcacagatgaaaacggtgtaaaaaaga(SEQ ID NO：141)
使用诺唯赞公司的Phanta Max Master Mix试剂分别扩增AcC2C9基因片段以及p15a质粒骨架，反应体系为：25μL 2xPhanta Max Master Mix，1.5μL5’Primer(10μM)，1.5μL 3’Primer(10μM)，0.5μl模板DNA(100ng/μL)，1.5μL DMSO，20μL ddH2O。PCR反应体系配好以后进行聚合酶链式反应(PCR)，循环如下：98℃2min；之后98℃20s，55℃20s，72℃3min，共30个循环；最后72℃5min。使用生工生物工程(上海)股份有限公司生产的SanPrep柱式PCR产物纯化试剂盒对PCR产物分别进行回收。PCR产物纯化的具体步骤按照试剂盒使用操作手册进行。
将得到的AcC2C9基因片段和p15a质粒骨架通过Gibson Assembly组装成一个质粒。具体的反应体系为：5μLNEBuilderHiFi DNA Assembly Master Mix(NEB公司)，20fmolAcC2C9基因片段，20fmol p15a质粒骨架，加入适量ddH2O至总体积为10μL。在50℃下反应1小时。将10μL反应产物转化到大肠杆菌感受态DH5α菌株中，并平铺在含有75μg/mL阿泊拉霉素的LB固体培养平板上。待转化液被固体培养平板吸收后于37℃培养箱倒置培养过夜。将培养平板上长出的转化菌株转接保存，并用生工生物工程(上海)股份有限公司的SanPrep柱式质粒DNA小量抽提试剂盒抽提质粒用于后续实验，质粒抽提的具体步骤按照试剂盒使用操作手册进行。同时将质粒送到生工生物工程(上海)股份有限公司进行测序确认，最终得到p15a-AcC2C9质粒。
pSGKP-AcC2C9质粒序列为SEQ ID NO:142，其具体构建方法如下：
在生工生物工程(上海)股份有限公司全基因合成AcC2C9核酸酶对应的引导RNA表达盒的DNA序列(其序列为SEQ ID NO:143)以及pSGKP质粒骨架(其序列为SEQ ID NO:144)。使用Gibson assembly技术将这两个DNA片段组装为pSGKP-AcC2C9质粒。转化大肠杆菌DH5α商品感受态细胞，涂布在带有50μg/mL卡那霉素的LB培养基平板上筛选。挑取单克隆后扩大培养，提取质粒，测序鉴定质粒序列后，获得pSGKP-AcC2C9质粒。
实施例五 构建靶向肺炎克雷伯菌基因组dha基因的pSGKP-AcC2C9-dha质粒
pSGKP-AcC2C9-dha质粒序列为SEQ ID NO:145，其具体构建方法如下：
先在肺炎克雷伯氏菌目标基因上选定某一AAG(本实施例中选择的AcC2C9识别PAM序列为AAG)序列后面20个碱基的DNA片段(这20个碱基被称为spacer，AAN不包含在其中)，然后将该spacer序列插入到实施例一中构建的p15a-AcC2C9Control质粒中。本步骤的特征在于：为使spacer片段能插入到p15a-AcC2C9Control质粒的BsaI位点中，需在该单链DNA序列的5’端加上atcg。同时需合成spacer的反补序列,并在反补序列5’端加上ggcc。例如本实施例选定的dha基因spacer的DNA序列(SEQ ID NO.146)为：5’-TGTTTGTTACCAACTGCCTG-3’，则两条引物的具体序列设计如下：
PAM primerF(dha-spF)：5’-atcgTGTTTGTTACCAACTGCCTG-3’(SEQ ID NO.147)
PAM primerR(dha-spR)：5’-ggccCAGGCAGTTGGTAACAAACA-3’(SEQ ID NO.148)
将上述两条引物进行退火，具体反应体系如下：5μl 10×T4 DNA ligase Buffer(NEB公司)，10μLPAM primerF(10μM)，10μLPAM primerR(10μM)，25μL ddH2O。在95℃加热5min，然后在1～2小时内缓慢地降低到室温，使两条单链引物通过碱基配对形成双链DNA。然后用ddH2O将得到的产物稀释20倍。
将上述得到的双链DNA通过Golden Gate技术插入实施例四中构建的pSGKP-AcC2C9质粒的BsaI位点，具体反应体系为：1μL 10×T4 DNA ligase Buffer，1μL上述20倍稀释的磷酸化双链DNA，20fmol pSGKP-AcC2C9质粒，0.5μL T4 DNA ligase(400units/μL)，0.5μLBsaI-HF(20units/μL)，最后加入适量ddH2O至总体积为10μL。该反应中所用Buffer和酶均由NEB公司生产。在PCR仪中进行反应，循环如下：37℃2min；16℃5min，共25个循环；然后80℃15min。
将该10μL反应产物转化到大肠杆菌感受态DH5α菌株中，并平铺在含有75μg/mL阿泊拉霉素的LB固体培养平板上。待转化液被固体培养平板吸收后于37℃培养箱倒置培养过夜。将培养基上长出的转化菌株转接保存，同时抽提质粒并送到生工生物工程(上海)股份有限公司进行测序验证，最终得到pSGKP-AcC2C9-dha质粒。
实施例六 制备含有p15a-AcC2C9质粒的肺炎克雷伯氏菌电转感受态
将肺炎克雷伯氏菌NCTC9633菌株(购自美国ATCC公司，货号：13883)在LB固体培养平板上划线，于37℃培养箱倒置培养过夜。挑取培养平板上长出的一个单克隆菌落，接种到3mL LB液体培养基中，在37℃摇床中以250rpm转速振摇过夜。第二天取1mL菌液接种到100mL新鲜的LB液体培养基中，在37℃摇床中继续振摇。当菌液的OD600达到0.3时，将菌液放在冰上冷却十分钟。在4℃离心机中以8000rpm转速离心5分钟收菌，弃去培养基上清，将底部的细菌沉淀用20mL 10％(v/v)甘油(高压灭菌，并在冰上预冷)重悬。以同样的转速离心，弃去上清，将底部的细菌沉淀再次用20mL 10％(v/v)甘油重悬。以同样的转速再一次离心，弃去上清后用1mL 10％(v/v)甘油重悬底部的细菌沉淀。将得到的细菌电转感受态分装到EP管中，每管50μL菌液。将分装的菌液先在液氮中快速冷冻，然后放入-80℃冰箱中保存。需要注意的是，在重悬时动作要轻柔，可以使用移液枪轻轻吹打的方式使细菌重悬。
取一管新鲜制备的肺炎克雷伯氏菌电转感受态，在冰上放置5～10分钟后，加入1μg实施例四中制备的p15a-AcC2C9质粒。混合均匀后转入1mm电转杯(Bio-Rad公司)，室温下在GenePulserXcell电击仪(Bio-Rad公司)中进行电击。电击参数为：1800V，200Ω，25μF。电击后立即加入1mL LB液体培养基，混合均匀后转入干净的EP管中，在37℃摇床中振摇1～2个小时。取100μL菌液平铺在含有75μg/mL阿泊拉霉素的LB固体培养平板上。待菌液被固体培养平板吸收后，于37℃培养箱倒置培养过夜。挑取一个单克隆菌落，在3mL含有75μg/mL阿泊拉霉素的LB液体培养基中于37℃摇床中以250rpm转速振摇过夜。第二天取1mL细菌菌液接种到100mL新鲜的含有75μg/mL阿泊拉霉素的LB培养基中，在37℃摇床中继续振摇。当菌液的OD600达到0.2时，在菌液中加入1mL 20％w/v的阿拉伯糖，并将菌液继续振摇2h后将菌液放在冰上冷却十分钟。在4℃离心机中以8000rpm转速离心5分钟收菌，弃去培养基上清，将底部的细菌沉淀用20mL 10％v/v甘油(高压灭菌，并在冰上预冷)重悬清洗两次。最后以同样的转速再一次离心，弃去上清后用1mL 10％v/v甘油重悬底部的细菌沉淀。将得到的细菌电转感受态分装到EP管中，每管50μL菌液。将分装的菌液先在液氮中快速冷冻，然后放入-80℃冰箱中保存。需要注意的是，在重悬时动作要轻柔，可以使用移液枪轻轻吹打的方式使细菌重悬。
实施例七 利用AcC2C9系统实现肺炎克雷伯氏菌活细胞高效基因删除
取一管含有实施例六制备的含有p15a-AcC2C9质粒的肺炎克雷伯氏菌电感受态细胞，在冰上放置5～10分钟，待其融化后加入200ng实施例五制备的pSGKP-AcC2C9-dha质粒和终浓度为5μM的dha基因同源重组修复模板(其序列为SEQ ID NO:149)，轻轻混匀。使用移液枪将混匀后的细菌质粒混合物转入预冷的1mm电转杯(Bio-Rad公司)，并在冰上静置5min。擦净电转杯外壁的冷凝水，在GenePulserXcell电击仪(Bio-Rad公司)中进行电击。电击参数为：1800V，200Ω，25μF。电击后立即加入1mL LB培养液洗出电击后的细胞，转入无菌的EP管中，在37℃摇床中以250rpm/min的转速复苏培养1h。将菌液涂布在含有75μg/mL阿泊拉霉素和50μg/mL卡那霉素的LB固体培养基上，待菌液被吸收后，于37℃培养箱中倒置培养过夜。
对长出的菌落进行菌落PCR验证编辑效果。
dha基因的菌落PCR验证的5’引物为：
5’-TCATCATTGGCATCCTGAAA-3’(SEQ ID NO.150)
dha基因的菌落PCR验证的3’引物为：
5’-TGGTCAGCCTGCTGATTAAA-3’(SEQ ID NO.151)
图2为采用CRISPR-AcC2C9系统在肺炎克雷伯氏菌活细胞中进行基因敲除的PCR结果图。结果显示，选定的dha基因可以被CRISPR-AcC2C9系统精准敲除。
实施例八AcC2C9蛋白异源表达质粒pET28a-sumo-AcC2C9的构建
以实施例一中的p15a-AcC2C9Control质粒为模板，扩增AcC2C9核酸酶基因序列，用于构建表达AcC2C9的pET28a-sumo-AcC2C9质粒(其序列为SEQ ID NO:152)。
扩增AcC2C9基因5’引物序列pET-AcC2C9F(5’Primer)：5’-tCTTGAAGTCCTCTTTCAGGGACCCATGGTGCAGACGGAGATCCTG-3’(SEQ ID NO：153)：
扩增AcC2C9基因3’引物序列pET-AcC2C9R(3’Primer)：
5’-gtcgacggagctcgaattcttaagcTTATGGAGGCGTTGCCGTTTCG-3’(SEQ ID NO：154)：
使用诺唯赞公司的Phanta Max Master Mix试剂扩增AcC2C9基因片段，反应体系为：25μL 2xPhanta Max Master Mix，1.5μL 5’Primer(10μM)，1.5μL3’Primer(10μM)，0.5μl实施例四中制备的p15a-AcC2C9质粒(100ng/μL)，1.5μLDMSO，20μL ddH2O。PCR反应体系配好以后进行聚合酶链式反应(PCR)，循环如下：98℃2min；之后98℃20s，55℃20s，72℃1min，共30个循环；最后72℃5min。使用生工生物工程(上海)股份有限公司生产的SanPrep柱式PCR产物纯化试剂盒对PCR产物分别进行回收。PCR产物纯化的具体步骤按照试剂盒使用操作手册进行。
在生工生物工程(上海)股份有限公司全基因合成pET28a-sumo质粒骨架(其序列为SEQ ID NO:155)。使用Gibson assembly技术将这两个AcC2C9基因片段和pET28a-sumo质粒骨架组装为pET28a-sumo-AcC2C9质粒。转化大肠杆菌DH5α商品感受态细胞，涂布在带有50μg/mL卡那霉素的LB培养基平板上筛选。挑取单克隆后扩大培养，提取质粒，测序鉴定质粒序列后，获得pET28a-sumo-AcC2C9质粒。
实施例九AcC2C9实现高效的体外双链DNA切割
切割底物制备。以肺炎克雷伯菌的基因组为模板，使用5’FAM标记的引物扩增切割底物。扩增切割底物的5’引物序列为：5’-CCGCATATATCGCAAAACAA-3’(SEQ ID NO：156)，扩增切割底物的3’引物序列为：5’-CTGGCTGTGAACAAAGTGGA-3’(SEQ ID NO：157)。使用生工生物工程(上海)股份有限公司生产的SanPrep柱式PCR产物纯化试剂盒对PCR产物分别进行回收。
AcC2C9核酸酶的制备。转化实施例八中构建的pET28a-sumo-AcC2C9质粒到大肠杆菌表达菌株BL21(DE3)。次日将转化子转接入1L的LB培养基中，37℃条件下进行震荡培养。当OD600到达0.6时，在培养液中加入0.5mL 1M IPTG,并将温度调低到16℃继续培养过夜。收集过夜后的菌株，超声破碎后，用HisTrap Ni-NTA(GE Healthcare)层析柱进行纯化。然后使用HiLoad 16/600Superdex 200pg分子筛(GE Healthcare)进一步纯化。纯化后的蛋白使用超滤管浓缩后保存在500mM NaCl，10mMTris-HCl，pH＝7.5，1mM DTT的缓冲液中。
靶向切割底物的完整引导RNA(其序列为SEQ ID NO：158)的制备。完整引导RNA的制备通过体外转录实现。其转录模板DNA由金唯智生物科技有限公司合成，其序列为SEQ IDNO:159。完整引导RNA用上述模板通过HiScribe T7 High Yield RNA Synthesis Kit(NEB公司)转录而成。具体步骤按照试剂盒使用操作手册进行。制备后的完整引导RNA经过酚氯仿抽提和乙醇沉淀进行纯化。
体外切割实验在150mM NaCl，10mM MgCl2，10mMTris-HCl，pH＝7.5，1mM DTT条件中进行。总反应体积为20μL，其中包括30nM切割底物，2μM AcC2C9和2μM完整引导RNA。反应时间分别为0分钟、1分钟、2分钟、5分钟、15分钟和30分钟，反应温度为37℃。反应结束后加入50mM EDTA和2mg/mL ProteinK终止反应。反应产物通过6％w/v的TBE丙烯酰胺凝胶进行分离，并在Bio-Rad公司的GELDOC凝胶成像仪中通过488nm蓝光激发后成像。
图3为采用AcC2C9核酸酶切割底物DNA的结果图。结果显示：底物DNA被AcC2C9精准切断成两段DNA片段。
实施例十 完整引导RNA拆分成tracrRNA和crRNA后的活性检测
靶向切割底物的完整引导RNA包括tracrRNA以及crRNA(与靶序列杂交的DNA-靶向区段与tracr配对物序列连接形成的RNA序列)。为了验证完整引导RNA拆分成tracrRNA和crRNA后是否还具有生物活性，本发明进行了体外双链DNA切割实验。
切割底物。同实施例九中所述。
AcC2C9核酸酶制备。同实施例九中所述。
tracrRNA(SEQ ID NO：160)的制备。tracrRNA的制备通过体外转录实现，其转录模板DNA由金唯智生物科技有限公司合成，其序列为SEQ ID NO:161。tracrRNA用上述模板通过HiScribe T7 High Yield RNA Synthesis Kit(NEB公司)转录而成。具体步骤按照试剂盒使用操作手册进行。制备后的tracrRNA经过酚氯仿抽提和乙醇沉淀进行纯化。
crRNA(SEQ ID NO：162)的制备。crRNA的制备通过体外转录实现，其转录模板DNA由金唯智生物科技有限公司合成，其序列为SEQ ID NO:163。crRNA的转录和纯化步骤与tracrRNA相同。
体外切割实验在150mM NaCl，10mM MgCl2，10mMTris-HCl，pH＝7.5，1mM DTT条件中进行。总反应体积为20μL，其中包括30nM切割底物，2μM AcC2C9，2μM tracrRNA和2μMcrRNA。反应时间为30分钟，反应温度为37℃。反应结束后加入50mM EDTA和2mg/mLProteinK终止反应。反应产物通过6％w/v的TBE丙烯酰胺凝胶进行分离，并在Bio-Rad公司的GELDOC凝胶成像仪中通过488nm蓝光激发后成像。可以看到在crRNA和tracrRNA条件下，底物DNA同样被精准切断成两段DNA片段，表明完整引导RNA拆分成tracrRNA和crRNA后同样具有生物活性。
图4(A)AcC2C9完整引导RNA的序列示意图(B)tracrRNA和crRNA条件下，用AcC2C9核酸酶切割底物DNA的结果图。
实施例十一AcC2C9切割双链DNA的切口位点鉴定
靶向于短荧光标记合成DNA的完整引导RNA制备。同实施例九中所述。
AcC2C9核酸酶制备。同实施例九中所述。
切割底物制备。切割底物为四种58bp的荧光标记合成DNA序列。由生工生物工程(上海)股份有限公司合成两条碱基互补配对的引物，其序列分别为5’-GGCTGTGAGAAAGCGGTTCAGGTGAAAGTGAAAACACTGCCCGACGCCCAGTTCGAAG-3’(SEQ ID NO:164)和5’-CTTCGAACTGGGCGTCGGGCAGTGTTTTCACTTTCACCTGAACCGCTTTCTCACAGCC-3”(SEQ ID NO:165)。分别在两条引物的5’端或者3’端标记FAM荧光基团。
体外切割实验在150mM NaCl，10mM MgCl2，10mM Tris-HCl，pH＝7.5，1mM DTT条件中进行。总反应体积为10μL，其中包括20nM切割底物，2μM AcC2C9,2μM完整引导RNA。反应温度为37℃。反应时间为30分钟。反应结束后加入10μL 2×甲酰胺上样缓冲液(生工生物工程(上海)股份有限公司)终止反应。反应产物通过20％的TBE-Urea-PAGE分离，并通过488nm蓝光激发后进行成像。
图5为采用AcC2C9核酸酶切割四种58bp的荧光标记DNA底物的结果图。结果显示：AcC2C9在靶向链的主要切割位点为PAM位点3’端下游的21-22nt处，而在非靶向链的主要切割位点为PAM位点3’端下游的15-16nt处。
实施例十一AcC2C9的哺乳动物细胞基因编辑质粒pAcC2C9Hs的构建
本实施例中所述的AcC2C9使用了较佳的针对人密码子优化的AcC2C9编码基因。
pAcC2C9Hs质粒序列为SEQ ID NO:166，其具体构建方法如下：
人源瞬时表达质粒骨架(其序列为SEQ ID NO:167)、嘌呤霉素抗性基因表达盒序列(其序列为SEQ ID NO:168)、针对人密码子优化的AcC2C9编码基因表达盒序列(其序列为SEQ ID NO:169)和人细胞中AcC2C9相应的引导RNA(SEQ ID NO:123)表达盒(其序列为SEQID NO:170)均由生工生物工程(上海)股份有限公司合成，四片段由Gibson assembly技术组装为pAcC2C9Hs质粒。转化大肠杆菌DH5α商品感受态细胞，涂布带有羧苄青霉素的LBA平板，挑取单克隆扩大培养，提取质粒，测序鉴定后，获得pAcC2C9Hs质粒。
实施例十二利用AcC2C9实现人类活细胞中的高效基因编辑
利用AcC2C9核酸酶可以实现在人活细胞中的高效基因编辑。以人胚胎肾细胞HEK293为模型展示其基因编辑效果。
向pAcC2C9Hs质粒中插入靶向spacer序列。本实施例中挑选了人基因组上的VEGFA、EMX1、HEXA、DNMT1、FANCF和PDCD1共5个基因上作为靶序列，并分别在这些靶序列上各挑选了1个20bp的靶向序列DNA。分别合成寡核苷酸序列Guide1-F、Guide1-R、Guide2-F、Guide2-R、Guide3-F、Guide3-R、Guide4-F、Guide4-R、Guide5-F和Guide5-R(序列分别为SEQID NO:171-180)。分别将Guide1-F和Guide1-R、Guide2-F和Guide2-R、Guide3-F和Guide3-R、Guide4-F和Guide4-R、以及Guide5-F和Guide5-R退火后，通过Golden gate assembly技术将这5个靶向序列DNA分别插入pAcC2C9Hs质粒中，分别构建成pAcC2C9Hs-guide1、pAcC2C9Hs-guide2、pAcC2C9Hs-guide3、pAcC2C9Hs-guide4和pAcC2C9Hs-guide5质粒(序列分别为SEQ ID NO:181-185)。
瞬时表达质粒介导的人细胞基因编辑。活化冻存的HEK293细胞。两天后传代HEK293细胞至24孔板中，每孔细胞数量约为1.5×105个。16-18小时后，用0.75μL的lipofectamine3000(Invitrogen)分别转染500ngpAcC2C9Hs-guide1、pAcC2C9Hs-guide2、pAcC2C9Hs-guide3、pAcC2C9Hs-guide4和pAcC2C9Hs-guide5质粒至细胞中。并继续培养72小时。消化贴壁细胞，提取基因组DNA。使用基因对应的扩增引物(Guide1-IndelF和Guide1-IndelR、Guide2-IndelF和Guide2-IndelR、Guide3-IndelF和Guide3-IndelR、Guide4-IndelF和Guide4-IndelR、Guide5-IndelF和Guide5-IndelR，其序列分别为SEQ ID NO:186-195)PCR扩增基因片段。胶回收PCR产物。用NEBuffer2(NEB公司)对PCR产物进行退火。而后加入T7endonuclease 1(NEB公司)，37℃酶切15min。加入50mM EDTA终止反应后，反应产物通过6％的TBE-PAGE分离，并通过4S Red dye(生工生物工程(上海)股份有限公司)进行染色成像。
图6为AcC2C9瞬时表达质粒介导的人细胞中的基因编辑结果图。(A)为Indel实验的TBE-PAGE胶图。在所选的5个不同基因位点中，AcC2C9核酸酶成功实现了对VEGFA、HEXA和DNMT1基因的高效基因编辑。(B)为AcC2C9瞬时表达质粒介导的人细胞中VEGFA基因编辑后高通量测序的统计结果图。如图所示，AcC2C9核酸酶可精确在靶序列处引入插入或缺失突变。
本发明中，序列信息如下：
SEQ ID NO.1
序列长度：76
序列种类：蛋白质
序列来源：Actinomadura craniellae
序列名称：AcC2C9蛋白结构域1
SEQ ID NO.2
序列长度：67
序列种类：蛋白质
序列来源：Actinomadura craniellae
序列名称：AcC2C9蛋白结构域2
SEQ ID NO.3
序列长度：506
序列种类：蛋白质
序列来源：Actinomadura craniellae
序列名称：AcC2C9
SEQ ID NO.4
序列长度：493
序列种类：蛋白质
序列来源：Candidatus Frankia meridionalis
序列名称：C2C9蛋白序列2
SEQ ID NO.5
序列长度：537
序列种类：蛋白质
序列来源：Corynebacterium glutamicum
序列名称：C2C9蛋白序列3
SEQ ID NO.6
序列长度：537
序列种类：蛋白质
序列来源：Dermacoccus sp.UBA1591
序列名称：C2C9蛋白序列4
SEQ ID NO.7
序列长度：492
序列种类：蛋白质
序列来源：Kitasatospora sp.NA04385
序列名称：C2C9蛋白序列5
SEQ ID NO.8
序列长度：553
序列种类：蛋白质
序列来源：Kocuria indica
序列名称：C2C9蛋白序列6
SEQ ID NO.9
序列长度：539
序列种类：蛋白质
序列来源：Kocuria sp.cx-455
序列名称：C2C9蛋白序列7
SEQ ID NO.10
序列长度：552
序列种类：蛋白质
序列来源：Mesorhizobium sp.B2-6-6
序列名称：C2C9蛋白序列8
SEQ ID NO.11
序列长度：485
序列种类：蛋白质
序列来源：Mycobacterium heckeshornense
序列名称：C2C9蛋白序列9
SEQ ID NO.12
序列长度：569
序列种类：蛋白质
序列来源：Micrococcus luteus
序列名称：C2C9蛋白序列10
SEQ ID NO.13
序列长度：505
序列种类：蛋白质
序列来源：Mycobacterium sp.MS1601
序列名称：C2C9蛋白序列11
SEQ ID NO.14
序列长度：561
序列种类：蛋白质
序列来源：Mycolicibacterium goodii
序列名称：C2C9蛋白序列12
SEQ ID NO.15
序列长度：496
序列种类：蛋白质
序列来源：Nocardiopsis metallicus
序列名称：C2C9蛋白序列13
SEQ ID NO.16
序列长度：499
序列种类：蛋白质
序列来源：Nocardiopsis synnemataformans DSM 44143
序列名称：C2C9蛋白序列14
SEQ ID NO.17
序列长度：560
序列种类：蛋白质
序列来源：Planctomycetes bacterium
序列名称：C2C9蛋白序列15
SEQ ID NO.18
序列长度：532
序列种类：蛋白质
序列来源：Propionimicrobium lymphophilum
序列名称：C2C9蛋白序列16
SEQ ID NO.19
序列长度：579
序列种类：蛋白质
序列来源：Pseudactinotalea sp.HY160
序列名称：C2C9蛋白序列17
SEQ ID NO.20
序列长度：540
序列种类：蛋白质
序列来源：Rothia dentocariosa
序列名称：C2C9蛋白序列18
SEQ ID NO.21
序列长度：549
序列种类：蛋白质
序列来源：Rothia dentocariosa
序列名称：C2C9蛋白序列19
SEQ ID NO.22
序列长度：541
序列种类：蛋白质
序列来源：Rothia kristinae
序列名称：C2C9蛋白序列20
SEQ ID NO.23
序列长度：558
序列种类：蛋白质
序列来源：Rothia mucilaginosa
序列名称：C2C9蛋白序列21
SEQ ID NO.24
序列长度：559
序列种类：蛋白质
序列来源：Rothia mucilaginosa
序列名称：C2C9蛋白序列22
SEQ ID NO.25
序列长度：559
序列种类：蛋白质
序列来源：Rothia mucilaginosa
序列名称：C2C9蛋白序列23
SEQ ID NO.26
序列长度：485
序列种类：蛋白质
序列来源：Rothia nasimurium
序列名称：C2C9蛋白序列24
SEQ ID NO.27
序列长度：562
序列种类：蛋白质
序列来源：Rothia sp.HMSC071C12
序列名称：C2C9蛋白序列25
SEQ ID NO.28
序列长度：494
序列种类：蛋白质
序列来源：Saccharomonospora piscinae
序列名称：C2C9蛋白序列26
SEQ ID NO.29
序列长度：606
序列种类：蛋白质
序列来源：Saccharopolyspora rectivirgula DSM 43747
序列名称：C2C9蛋白序列27
SEQ ID NO.30
序列长度：459
序列种类：蛋白质
序列来源：Streptomyces albidus
序列名称：C2C9蛋白序列28
SEQ ID NO.31
序列长度：517
序列种类：蛋白质
序列来源：Streptomyces bauhiniae
序列名称：C2C9蛋白序列29
SEQ ID NO.32
序列长度：469
序列种类：蛋白质
序列来源：Streptomyces filamentosus
序列名称：C2C9蛋白序列30
SEQ ID NO.33
序列长度：583
序列种类：蛋白质
序列来源：Streptomyces griseocarneus
序列名称：C2C9蛋白序列31
SEQ ID NO.34
序列长度：466
序列种类：蛋白质
序列来源：Streptomyces longispororuber
序列名称：C2C9蛋白序列32
SEQ ID NO.35
序列长度：463
序列种类：蛋白质
序列来源：Streptomyces lydicus
序列名称：C2C9蛋白序列33
SEQ ID NO.36
序列长度：511
序列种类：蛋白质
序列来源：Streptomyces netropsis
序列名称：C2C9蛋白序列34
SEQ ID NO.37
序列长度：519
序列种类：蛋白质
序列来源：Streptomyces niveus NCIMB 11891
序列名称：C2C9蛋白序列35
SEQ ID NO.38
序列长度：515
序列种类：蛋白质
序列来源：Streptomyces rimosus subsp.rimosus
序列名称：C2C9蛋白序列36
SEQ ID NO.39
序列长度：518
序列种类：蛋白质
序列来源：Streptomyces sp.CB04723
序列名称：C2C9蛋白序列37
SEQ ID NO.40
序列长度：532
序列种类：蛋白质
序列来源：Streptomyces sp.CB04723
序列名称：C2C9蛋白序列38
SEQ ID NO.41
序列长度：513
序列种类：蛋白质
序列来源：Streptomyces sp.CNH099
序列名称：C2C9蛋白序列39
SEQ ID NO.42
序列长度：522
序列种类：蛋白质
序列来源：Streptomyces sp.CNS606
序列名称：C2C9蛋白序列40
SEQ ID NO.43
序列长度：519
序列种类：蛋白质
序列来源：Streptomyces sp.IB2014 016-6
序列名称：C2C9蛋白序列41
SEQ ID NO.44
序列长度：518
序列种类：蛋白质
序列来源：Streptomyces sp.man185
序列名称：C2C9蛋白序列42
SEQ ID NO.45
序列长度：506
序列种类：蛋白质
序列来源：Streptomyces sp.S4
序列名称：C2C9蛋白序列43
SEQ ID NO.46
序列长度：477
序列种类：蛋白质
序列来源：Streptomyces sp.SID2563
序列名称：C2C9蛋白序列44
SEQ ID NO.47
序列长度：509
序列种类：蛋白质
序列来源：Streptomyces sp.UH6
序列名称：C2C9蛋白序列45
SEQ ID NO.48
序列长度：468
序列种类：蛋白质
序列来源：Streptomyces spororaveus
序列名称：C2C9蛋白序列46
SEQ ID NO.49
序列长度：462
序列种类：蛋白质
序列来源：Streptomyces xanthochromogenes
序列名称：C2C9蛋白序列47
SEQ ID NO.50
序列长度：530
序列种类：蛋白质
序列来源：Streptosporangium violaceochromogenes
序列名称：C2C9蛋白序列48
SEQ ID NO.51
序列长度：512
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列49
SEQ ID NO.52
序列长度：758
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列50
SEQ ID NO.53
序列长度：669
序列种类：蛋白质
序列来源：Cellulosimicrobium cellulans
序列名称：C2C9蛋白序列51
SEQ ID NO.54
序列长度：531
序列种类：蛋白质
序列来源：Rhodococcus hoagii
序列名称：C2C9蛋白序列52
SEQ ID NO.55
序列长度：527
序列种类：蛋白质
序列来源：Rhodococcus sp.OK519
序列名称：C2C9蛋白序列53
SEQ ID NO.56
序列长度：560
序列种类：蛋白质
序列来源：Rothia sp.HMSC071C12
序列名称：C2C9蛋白序列54
SEQ ID NO.57
序列长度：496
序列种类：蛋白质
序列来源：Nocardiopsis alba
序列名称：C2C9蛋白序列55
SEQ ID NO.58
序列长度：500
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列56
SEQ ID NO.59
序列长度：539
序列种类：蛋白质
序列来源：Streptosporangium subroseum
序列名称：C2C9蛋白序列57
SEQ ID NO.60
序列长度：595
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列58
SEQ ID NO.61
序列长度：631
序列种类：蛋白质
序列来源：Streptomyces agglomeratus
序列名称：C2C9蛋白序列59
SEQ ID NO.62
序列长度：505
序列种类：蛋白质
序列来源：Murinocardiopsis flavida
序列名称：C2C9蛋白序列60
SEQ ID NO.63
序列长度：566
序列种类：蛋白质
序列来源：Streptomyces sp.NRRL F-2664
序列名称：C2C9蛋白序列61
SEQ ID NO.64
序列长度：715
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列62
SEQ ID NO.65
序列长度：529
序列种类：蛋白质
序列来源：Cellulomonas marina
序列名称：C2C9蛋白序列63
SEQ ID NO.66
序列长度：449
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列64
SEQ ID NO.67
序列长度：577
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列65
SEQ ID NO.68
序列长度：582
序列种类：蛋白质
序列来源：Miniimonas sp.S16
序列名称：C2C9蛋白序列66
SEQ ID NO.69
序列长度：509
序列种类：蛋白质
序列来源：Streptomyces sp.SPB074
序列名称：C2C9蛋白序列67
SEQ ID NO.70
序列长度：509
序列种类：蛋白质
序列来源：Streptomyces yunnanensis
序列名称：C2C9蛋白序列68
SEQ ID NO.71
序列长度：760
序列种类：蛋白质
序列来源：Mycobacteroides abscessus
序列名称：C2C9蛋白序列69
SEQ ID NO.72
序列长度：494
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列70
SEQ ID NO.73
序列长度：545
序列种类：蛋白质
序列来源：Streptomyces sp.CB02009
序列名称：C2C9蛋白序列71
SEQ ID NO.74
序列长度：433
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列72
SEQ ID NO.75
序列长度：681
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列73
SEQ ID NO.76
序列长度：528
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列74
SEQ ID NO.77
序列长度：741
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列75
SEQ ID NO.78
序列长度：611
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列76
SEQ ID NO.79
序列长度：594
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列77
SEQ ID NO.80
序列长度：593
序列种类：蛋白质
序列来源：Kitasatospora mediocidica
序列名称：C2C9蛋白序列78
SEQ ID NO.81
序列长度：493
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列79
SEQ ID NO.82
序列长度：612
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列80
SEQ ID NO.83
序列长度：506
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列81
SEQ ID NO.84
序列长度：607
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列82
SEQ ID NO.85
序列长度：572
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列83
SEQ ID NO.86
序列长度：508
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列84
SEQ ID NO.87
序列长度：542
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列85
SEQ ID NO.88
序列长度：564
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列86
SEQ ID NO.89
序列长度：498
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列87
SEQ ID NO.90
序列长度：538
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列88
SEQ ID NO.91
序列长度：659
序列种类：蛋白质
序列来源：Streptomyces sp.
序列名称：C2C9蛋白序列89
SEQ ID NO.92
序列长度：526
序列种类：蛋白质
序列来源：Quadrisphaera granulorum
序列名称：C2C9蛋白序列90
SEQ ID NO.93
序列长度：549
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列91
SEQ ID NO.94
序列长度：533
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列92
SEQ ID NO.95
序列长度：436
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列93
SEQ ID NO.96
序列长度：483
序列种类：蛋白质
序列来源：Prauserella rugosa
序列名称：C2C9蛋白序列94
SEQ ID NO.97
序列长度：578
序列种类：蛋白质
序列来源：Rothia sp.HMSC061D12
序列名称：C2C9蛋白序列95
SEQ ID NO.98
序列长度：519
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列96
SEQ ID NO.99
序列长度：440
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列97
SEQ ID NO.100
序列长度：495
序列种类：蛋白质
序列来源：Mycobacterium grossiae
序列名称：C2C9蛋白序列98
SEQ ID NO.101
序列长度：494
序列种类：蛋白质
序列来源：Mycobacterium avium
序列名称：C2C9蛋白序列99
SEQ ID NO.102
序列长度：548
序列种类：蛋白质
序列来源：Corynebacterium maris
序列名称：C2C9蛋白序列100
SEQ ID NO.103
序列长度：462
序列种类：蛋白质
序列来源：Streptomyces sp.NRRL S-378
序列名称：C2C9蛋白序列101
SEQ ID NO.104
序列长度：492
序列种类：蛋白质
序列来源：Kitasatospora cheerisanensis KCTC 2395
序列名称：C2C9蛋白序列102
SEQ ID NO.105
序列长度：495
序列种类：蛋白质
序列来源：Mycobacterium sp.SWH-M3
序列名称：C2C9蛋白序列103
SEQ ID NO.106
序列长度：524
序列种类：蛋白质
序列来源：Streptomyces roseochromogenus
序列名称：C2C9蛋白序列104
SEQ ID NO.107
序列长度：513
序列种类：蛋白质
序列来源：Mycobacterium intracellulare subsp.yongonense 05-1390
序列名称：C2C9蛋白序列105
SEQ ID NO.108
序列长度：632
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列106
SEQ ID NO.109
序列长度：495
序列种类：蛋白质
序列来源：Mycobacterium sp.JS623
序列名称：C2C9蛋白序列107
SEQ ID NO.110
序列长度：499
序列种类：蛋白质
序列来源：Mycolicibacterium fallax
序列名称：C2C9蛋白序列108
SEQ ID NO.111
序列长度：449
序列种类：蛋白质
序列来源：Nocardiopsis sp.JB363
序列名称：C2C9蛋白序列109
SEQ ID NO.112
序列长度：726
序列种类：蛋白质
序列来源：Rothia sp.HMSC069C03
序列名称：C2C9蛋白序列110
SEQ ID NO.113
序列长度：494
序列种类：蛋白质
序列来源：Mycobacterium avium
序列名称：C2C9蛋白序列111
SEQ ID NO.114
序列长度：522
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列112
SEQ ID NO.115
序列长度：653
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列113
SEQ ID NO.116
序列长度：427
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列114
SEQ ID NO.117
序列长度：474
序列种类：蛋白质
序列来源：宏基因组测序
序列名称：C2C9蛋白序列115
SEQ ID NO.118
序列长度：29
序列种类：RNA
序列来源：Actinomadura craniellae
序列名称：AcC2C9的tracr配对物序列
SEQ ID NO.119
序列长度：250
序列种类：RNA
序列来源：Actinomadura craniellae
序列名称：AcC2C9的tracr RNA序列
SEQ ID NO.120
序列长度：279
序列种类：RNA
序列来源：Actinomadura craniellae
序列名称：AcC2C9的引导RNA骨架序列
SEQ ID NO.121
序列长度：1518
序列种类：DNA
序列来源：Actinomadura craniellae
序列名称：AcC2C9核酸酶碱基序列
SEQ ID NO.122
序列长度：1518
序列种类：DNA
序列来源：Artificial Sequence
序列名称：AcC2C9核酸酶人类密码子优化碱基序列
SEQ ID NO.123
序列长度：279
序列种类：RNA
序列来源：Artificial Sequence
序列名称：实施例中细胞实验所使用AcC2C9的gRNA序列
SEQ ID NO.124
序列长度：8198
序列种类：DNA
序列来源：Artificial Sequence
序列名称：p15a-AcC2C9Control
SEQ ID NO.125
序列长度：1952
序列种类：DNA
序列来源：Artificial Sequence
序列名称：AcC2C9-gRNA-expression
SEQ ID NO.126
序列长度：6296
序列种类：DNA
序列来源：Artificial Sequence
序列名称：p15a-backbone
SEQ ID NO.127
序列长度：8201
序列种类：DNA
序列来源：Artificial Sequence
序列名称：p15a-AcC2C9PAM
SEQ ID NO.128
序列长度：24
序列种类：DNA
序列来源：Artificial Sequence
序列名称：PAM primerF
SEQ ID NO.129
序列长度：24
序列种类：DNA
序列来源：Artificial Sequence
序列名称：PAM primerR
SEQ ID NO.130
序列长度：2707
序列种类：DNA
序列来源：Artificial Sequence
序列名称：pUC19-6N-library
SEQ ID NO.131
序列长度：46
序列种类：DNA
序列来源：Artificial Sequence
序列名称：6N-F
SEQ ID NO.132
序列长度：30
序列种类：DNA
序列来源：Artificial Sequence
序列名称：6N-R
SEQ ID NO.133
序列长度：51
序列种类：DNA
序列来源：Artificial Sequence
序列名称：PAM-1F
SEQ ID NO.134
序列长度：43
序列种类：DNA
序列来源：Artificial Sequence
序列名称：PAM-1R
SEQ ID NO.135
序列长度：66
序列种类：DNA
序列来源：Artificial Sequence
序列名称：PAM-2F
SEQ ID NO.136
序列长度：63
序列种类：DNA
序列来源：Artificial Sequence
序列名称：PAM-2R
SEQ ID NO.137
序列长度：8037
序列种类：DNA
序列来源：Artificial Sequence
序列名称：p15a-AcC2C9
SEQ ID NO.138
序列长度：41
序列种类：DNA
序列来源：Artificial Sequence
序列名称：AcC2C9F
SEQ ID NO.139
序列长度：36
序列种类：DNA
序列来源：Artificial Sequence
序列名称：ACC2C9R
SEQ ID NO.140
序列长度：33
序列种类：DNA
序列来源：Artificial Sequence
序列名称：p15aF
SEQ ID NO.141
序列长度：43
序列种类：DNA
序列来源：Artificial Sequence
序列名称：p15aR
SEQ ID NO.142
序列长度：5040
序列种类：DNA
序列来源：Artificial Sequence
序列名称：pSGKP-AcC2C9
SEQ ID NO.143
序列长度：496
序列种类：DNA
序列来源：Artificial Sequence
序列名称：J23119-AcgRNA
SEQ ID NO.144
序列长度：4594
序列种类：DNA
序列来源：Artificial Sequence
序列名称：pSGKP-backbone
SEQ ID NO.145
序列长度：5043
序列种类：DNA
序列来源：Artificial Sequence
序列名称：pSGKP-AcC2C9-dha
SEQ ID NO.146
序列长度：20
序列种类：DNA
序列来源：Artificial Sequence
序列名称：dha-spacer
SEQ ID NO.147
序列长度：24
序列种类：DNA
序列来源：Artificial Sequence
序列名称：dha-spF
SEQ ID NO.148
序列长度：24
序列种类：DNA
序列来源：Artificial Sequence
序列名称：dha-spR
SEQ ID NO.149
序列长度：90
序列种类：DNA
序列来源：Artificial Sequence
序列名称：dha基因同源重组修复模板
SEQ ID NO.150
序列长度：20
序列种类：DNA
序列来源：Artificial Sequence
序列名称：dha基因的菌落PCR验证的5'引物
SEQ ID NO.151
序列长度：20
序列种类：DNA
序列来源：Artificial Sequence
序列名称：dha基因的菌落PCR验证的3'引物
SEQ ID NO.152
序列长度：7178
序列种类：DNA
序列来源：Artificial Sequence
序列名称：pET28a-sumo-AcC2C9
SEQ ID NO.153
序列长度：46
序列种类：DNA
序列来源：Artificial Sequence
序列名称：pET-AcC2C9F
SEQ ID NO.154
序列长度：47
序列种类：DNA
序列来源：Artificial Sequence
序列名称：pET-AcC2C9R
SEQ ID NO.155
序列长度：5657
序列种类：DNA
序列来源：Artificial Sequence
序列名称：pET28a-sumo-backbone
SEQ ID NO.156
序列长度：20
序列种类：DNA
序列来源：
序列名称：DNA-substrate-F
SEQ ID NO.157
序列长度：20
序列种类：DNA
序列来源：Artificial Sequence
序列名称：DNA-substrate-R
SEQ ID NO.158
序列长度：254
序列种类：RNA
序列来源：Artificial Sequence
序列名称：靶向切割底物的完整引导RNA
SEQ ID NO.159
序列长度：274
序列种类：DNA
序列来源：Artificial Sequence
序列名称：gRNA-template
SEQ ID NO.160
序列长度：205
序列种类：RNA
序列来源：Artificial Sequence
序列名称：AcC2C9的tracrRNA序列
SEQ ID NO.161
序列长度：225
序列种类：DNA
序列来源：Artificial Sequence
序列名称：AcC2C9 tracrRNA的转录模板
SEQ ID NO.162
序列长度：49
序列种类：RNA
序列来源：Artificial Sequence
序列名称：靶向切割底物的AcC2C9 crRNA序列
SEQ ID NO.163
序列长度：69
序列种类：DNA
序列来源：Artificial Sequence
序列名称：AcC2C9 crRNA的转录模板
SEQ ID NO.164
序列长度：58
序列种类：DNA
序列来源：Artificial Sequence
序列名称：58bp-DNA-substrate F
SEQ ID NO.165
序列长度：58
序列种类：DNA
序列来源：Artificial Sequence
序列名称：58bp-DNA-substrate R
SEQ ID NO.166
序列长度：7204
序列种类：DNA
序列来源：Artificial Sequence
序列名称：pAcC2C9Hs
SEQ ID NO.167
序列长度：2491
序列种类：DNA
序列来源：Artificial Sequence
序列名称：人源瞬时表达质粒骨架
SEQ ID NO.168
序列长度：1566
序列种类：DNA
序列来源：Artificial Sequence
序列名称：嘌呤霉素抗性基因表达盒序列
SEQ ID NO.169
序列长度：2474
序列种类：DNA
序列来源：Artificial Sequence
序列名称：human-AcC2C9
SEQ ID NO.170
序列长度：673
序列种类：DNA
序列来源：Artificial Sequence
序列名称：human-AcC2C9-gRNA
SEQ ID NO.171
序列长度：24
序列种类：DNA
序列来源：Artificial Sequence
序列名称：Guide1-F
SEQ ID NO.172
序列长度：24
序列种类：DNA
序列来源：Artificial Sequence
序列名称：Guide1-R
SEQ ID NO.173
序列长度：24
序列种类：DNA
序列来源：Artificial Sequence
序列名称：Guide2-F
SEQ ID NO.174
序列长度：24
序列种类：DNA
序列来源：Artificial Sequence
序列名称：Guide2-R
SEQ ID NO.175
序列长度：24
序列种类：DNA
序列来源：Artificial Sequence
序列名称：Guide3-F
SEQ ID NO.176
序列长度：24
序列种类：DNA
序列来源：Artificial Sequence
序列名称：Guide3-R
SEQ ID NO.177
序列长度：24
序列种类：DNA
序列来源：Artificial Sequence
序列名称：Guide4-F
SEQ ID NO.178
序列长度：24
序列种类：DNA
序列来源：Artificial Sequence
序列名称：Guide4-R
SEQ ID NO.179
序列长度：24
序列种类：DNA
序列来源：Artificial Sequence
序列名称：Guide5-F
SEQ ID NO.180
序列长度：24
序列种类：DNA
序列来源：Artificial Sequence
序列名称：Guide5-R
SEQ ID NO.181
序列长度：7198
序列种类：DNA
序列来源：Artificial Sequence
序列名称：pAcC2C9Hs-guide1
SEQ ID NO.182
序列长度：7198
序列种类：DNA
序列来源：Artificial Sequence
序列名称：pAcC2C9Hs-guide2
SEQ ID NO.183
序列长度：7198
序列种类：DNA
序列来源：Artificial Sequence
序列名称：pAcC2C9Hs-guide3
SEQ ID NO.184
序列长度：7198
序列种类：DNA
序列来源：Artificial Sequence
序列名称：pAcC2C9Hs-guide4
SEQ ID NO.185
序列长度：7198
序列种类：DNA
序列来源：Artificial Sequence
序列名称：pAcC2C9Hs-guide5
SEQ ID NO.186
序列长度：20
序列种类：DNA
序列来源：Artificial Sequence
序列名称：Guide1-IndelF
SEQ ID NO.187
序列长度：20
序列种类：DNA
序列来源：Artificial Sequence
序列名称：Guide1-IndelR
SEQ ID NO.188
序列长度：22
序列种类：DNA
序列来源：Artificial Sequence
序列名称：Guide2-IndelF
SEQ ID NO.189
序列长度：22
序列种类：DNA
序列来源：Artificial Sequence
序列名称：Guide2-IndelR
SEQ ID NO.190
序列长度：23
序列种类：DNA
序列来源：Artificial Sequence
序列名称：Guide3-IndelF
SEQ ID NO.191
序列长度：23
序列种类：DNA
序列来源：Artificial Sequence
序列名称：Guide3-IndelR
SEQ ID NO.192
序列长度：22
序列种类：DNA
序列来源：Artificial Sequence
序列名称：Guide4-IndelF
SEQ ID NO.193
序列长度：22
序列种类：DNA
序列来源：Artificial Sequence
序列名称：Guide4-IndelR
SEQ ID NO.194
序列长度：22
序列种类：DNA
序列来源：Artificial Sequence
序列名称：Guide5-IndelF
SEQ ID NO.195
序列长度：22
序列种类：DNA
序列来源：Artificial Sequence
序列名称：Guide5-IndelR
以上实施例仅用以说明本发明的技术方案而非对其限制；尽管参照较佳实施例对本发明进行了详细的说明，所属领域的普通技术人员应当理解：依然可以对本发明的具体实施方式进行修改或者对部分技术特征进行等同替换；在不背离发明构思的精神和范围下，本领域技术人员能够想到的变化和优点均应涵盖在本发明请求保护的技术方案范围当中。
序列表
<110> 上海科技大学
<120> 一种基于C2C9核酸酶的新型基因组编辑系统及其应用
<160> 195
<170> SIPOSequenceListing 1.0
<210> 1
<211> 76
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 1
Leu His Gln Ile Thr Lys Arg Leu Thr Thr Gly Phe Ala Val Val Ala
1 5 10 15
Leu Glu Asp Leu Asn Val Ala Gly Met Thr Arg Ser Ala Arg Gly Thr
20 25 30
Val Ala Ala Pro Gly Lys Asn Val Arg Gln Lys Ala Gly Leu Asn Arg
35 40 45
Val Ile Leu Asp Ser Ala Pro Ala Glu Leu Arg Arg Gln Val Asn Tyr
50 55 60
Lys Ala Thr Trp Tyr Gly Ser Glu Leu Ala Val Ala
65 70 75
<210> 2
<211> 67
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 2
Ala Glu Leu Arg Arg Gln Val Asn Tyr Lys Ala Thr Trp Tyr Gly Ser
1 5 10 15
Glu Leu Ala Val Ala Asp Arg Trp Phe Pro Ser Ser Lys Thr Cys Ser
20 25 30
Gly Cys Gly Trp Gln Asn Pro His Leu Lys Leu Ser Asp Arg Val Phe
35 40 45
Arg Cys Thr Asp Cys Gly Leu Val Met Asp Arg Asp Met Asn Ala Ala
50 55 60
Arg Asn Ile
65
<210> 3
<211> 506
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 3
Met Val Gln Thr Glu Ile Leu Lys Ala Phe Arg Phe Ala Leu Asp Pro
1 5 10 15
Thr Ser Val Gln Val Ala Ala Leu Ser Arg His Ala Gly Ala Ala Arg
20 25 30
Trp Ala Phe Asn His Ala Leu Ala Ala Lys Val Gly Ala His Glu Arg
35 40 45
Trp Arg Ala Glu Val Ala Lys Leu Val Gly Asp Gly Val Pro Glu Glu
50 55 60
Gln Ala Arg Arg Gln Val Arg Val Pro Val Pro Met Lys Pro Ala Ile
65 70 75 80
Gln Lys Ala Leu Asn Ala Val Lys Gly Asp Ser Arg Lys Gly Leu Asp
85 90 95
Gly Ala Cys Pro Trp Trp His Glu Val Asn Thr Tyr Ala Phe Gln Ser
100 105 110
Ala Phe Ile Asp Ala Asp Gln Ala Trp Lys Asn Trp Leu Asp Ser Leu
115 120 125
Ser Gly Lys Arg Val Gly Arg Arg Val Gly Tyr Pro Arg Phe Lys Lys
130 135 140
Lys Gly Arg Ala Arg Asp Ser Phe Arg Leu His His Asp Val Lys Lys
145 150 155 160
Pro Ser Ile Arg Leu Ala Gly Tyr Arg Arg Leu Arg Leu Pro Arg Ile
165 170 175
Gly Glu Val Arg Leu His Asp Ser Gly Lys Arg Leu Ala Arg Leu Ile
180 185 190
Asp Arg Gly Asp Ala Val Val Gln Ser Val Thr Val Ser Arg Gly Gly
195 200 205
His Arg Trp Tyr Ala Ser Val Leu Cys Lys Val Thr Val Gln Val Pro
210 215 220
Asp Arg Pro Ser Arg Arg Gln Arg Glu Arg Gly Ala Val Gly Val Asp
225 230 235 240
Leu Gly Val Lys Val Leu Ala Ala Leu Ser Lys Pro Leu Val Val Asp
245 250 255
Asp Pro Ser Ser Ala Leu Val Arg Asn Pro Gln His Leu Arg Gln Ala
260 265 270
Glu Arg Arg Leu Leu Lys Ala Gln Arg Ala Leu Ala Arg Thr Gln Lys
275 280 285
Gly Ser Ala Arg Arg Glu Lys Ala Lys Arg Arg Val Gly Arg Ala His
290 295 300
His Glu Val Ala Val Arg Arg His Ala Ala Leu His Gln Ile Thr Lys
305 310 315 320
Arg Leu Thr Thr Gly Phe Ala Val Val Ala Leu Glu Asp Leu Asn Val
325 330 335
Ala Gly Met Thr Arg Ser Ala Arg Gly Thr Val Ala Ala Pro Gly Lys
340 345 350
Asn Val Arg Gln Lys Ala Gly Leu Asn Arg Val Ile Leu Asp Ser Ala
355 360 365
Pro Ala Glu Leu Arg Arg Gln Val Asn Tyr Lys Ala Thr Trp Tyr Gly
370 375 380
Ser Glu Leu Ala Val Ala Asp Arg Trp Phe Pro Ser Ser Lys Thr Cys
385 390 395 400
Ser Gly Cys Gly Trp Gln Asn Pro His Leu Lys Leu Ser Asp Arg Val
405 410 415
Phe Arg Cys Thr Asp Cys Gly Leu Val Met Asp Arg Asp Met Asn Ala
420 425 430
Ala Arg Asn Ile Glu Arg His Ala Val Leu Ile Asp Arg Asn Val Ala
435 440 445
Cys Asp Arg Arg Glu Thr Leu Asn Ala Arg Gly Ala Ser Ile Arg Pro
450 455 460
Thr Thr Ser Arg Gly Gly Arg His Asp Ala Ser Lys Arg Glu Gly Ser
465 470 475 480
Gly Ala Arg Pro Glu Ser Pro Gln Gln Ser Asp Leu Leu Thr Leu Pro
485 490 495
Thr Leu Asn Asn Glu Thr Ala Thr Pro Pro
500 505
<210> 4
<211> 493
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 4
Met Ser Gly Ala Ile Gln Arg Ser His Ala Thr Glu Thr Arg Glu Val
1 5 10 15
Leu Arg Ala Tyr Arg Val Thr Leu Asp Pro Thr Pro Asp Gln Glu Ser
20 25 30
Met Leu Ala Gln His Ala Gly Ala Ala Arg Trp Ala Phe Asn His Ala
35 40 45
Leu Ala Ala Lys Val Asp Ala His Glu Arg Trp Lys Thr Ala Val Ala
50 55 60
Glu Leu Val Ala Ala Gly Val Pro Glu Glu Ala Ala Arg Lys Lys Ile
65 70 75 80
Lys Val Leu Val Pro Gly Lys Gln Val Ile Gln Lys Ala Leu Asn Ala
85 90 95
Val Lys Gly Asp Asp Arg Lys Gly Ile Asp Gly Ala Cys Pro Trp Trp
100 105 110
His Thr Val Ser Thr Tyr Ala Phe Gln Ser Ala Phe Leu Asp Ala Asp
115 120 125
Met Ala Trp Lys Ala Trp Leu Asp Ser Leu Thr Gly Arg Arg Lys Gly
130 135 140
Arg Arg Val Gly Tyr Pro Arg Phe Lys Lys Lys Gly Arg Ser Arg Asp
145 150 155 160
Ser Phe Arg Leu His His Asp Val Lys Arg Pro Thr Ile Arg Pro Ala
165 170 175
Thr Thr Arg Arg Leu Leu Leu Pro Arg Ile Gly Glu Val Arg Thr His
180 185 190
Asp Ser Leu Lys Arg Leu Lys Ala Ala Leu Gly Arg Arg Ser Gly Val
195 200 205
Ile Gln Ser Val Thr Ile Ala Arg Gly Gly His Arg Trp Tyr Ala Ser
210 215 220
Val Leu Val Arg Glu Gln Met Leu Met Pro Ala Pro Thr Arg Ala Gln
225 230 235 240
Gln Ala Ala Gly Thr Val Gly Val Asp Leu Gly Val His Ser Met Ala
245 250 255
Ala Leu Ser Thr Gly Glu Ile Ile Pro Asn Pro Arg Val Lys Ala Arg
260 265 270
His Ala Pro Ala Leu Ala Lys Ala Ser Arg Ala Tyr Ala Arg Thr Val
275 280 285
Lys Gly Ser Arg Arg Arg Glu Gln Ala Arg Arg Arg Leu Ala Arg Leu
290 295 300
His His Arg Glu Ala Thr Arg Arg Ser Thr Gly Leu His His Leu Thr
305 310 315 320
Lys Arg Leu Ala Thr Gly Trp Ala Val Val Ala Val Glu Asp Leu Asp
325 330 335
Val Ala Gly Leu Ile Arg Ser Ala Lys Gly Thr Val Asp Lys Pro Gly
340 345 350
Arg Arg Val Arg Gln Lys Ser Gly Leu Asn Arg Ser Ile Leu Asp Val
355 360 365
Ser Pro Gly Glu Val Arg Arg Gln Leu Asp Tyr Lys Thr Gly Trp Tyr
370 375 380
Gly Ser Thr Ile Ala Ile Leu Asp Arg Phe Tyr Pro Ser Ser Lys Thr
385 390 395 400
Cys Ser Gly Cys Gly Gly Arg Lys Pro Asn Leu Thr Leu Ser Glu Arg
405 410 415
Val Phe Ile Cys Thr Ile Cys Gly Leu Ser Met Asp Cys Asp Val Asn
420 425 430
Ala Ala Val Asn Ile Ala Arg Phe Ala Val Ala Ser Asp Arg Gly Glu
435 440 445
Thr Leu Asn Ala Arg Gly Ala Ser Val Arg Pro Ser His Leu Gln Val
450 455 460
Gly Arg Pro Asp Ala Leu Met Arg Glu Asp Pro Pro Glu Gly Gly Pro
465 470 475 480
Pro Gln His Ser Asp Ala Leu Ala Ser Ser Thr Pro Thr
485 490
<210> 5
<211> 537
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 5
Met Thr Ser Thr Thr Leu Ala Pro Glu Glu Pro Leu Met Val Phe Arg
1 5 10 15
Gly Ala Arg Phe Arg Leu Asp Pro Thr Gly Glu Gln Gln Gly Ile Leu
20 25 30
Ser Gln Gln Ala Gly Ala Ala Arg Val Ala Tyr Asn Met Met Cys Thr
35 40 45
Leu Asn Lys Asp Ile Leu Glu Ala Arg Ser Gln Leu Tyr Ser Thr Leu
50 55 60
Ile Lys Asp Gly Lys Thr Lys Asp Lys Ala Lys Lys Glu Leu Lys Ala
65 70 75 80
Ala Ala Lys Glu Asp Pro Ser Leu Ala Ile Val Trp Ala Arg Asp Phe
85 90 95
Asp Lys Asn Tyr Ile Thr Pro Glu Arg Asn Arg His Lys His Ala Ala
100 105 110
Gln Arg Ile Ala Ala Gly Glu Asn Pro Val Asp Val Trp Asn Pro Asp
115 120 125
Glu Glu Arg Phe Asn Glu Pro Trp Leu His Thr Ala Asn Arg Arg Val
130 135 140
Leu Arg Ser Gly Gln Lys Gln Tyr Glu Gln Ala Leu Asp Asn Phe Phe
145 150 155 160
Lys Ser Gln Asn Gly Ser Arg Ala Gly Gln Lys Met Gly Lys Pro Arg
165 170 175
Phe Lys Thr Lys Ile Arg Ser Thr Asp Ser Phe Thr Ile Asp Ala Val
180 185 190
Asp Val Ser Ser Ser Thr Thr Leu Ile Arg Asp Ile Gly Pro Lys Asp
195 200 205
His Ala Arg Tyr Lys Thr Gly Glu Ala Ser Thr Gly Ile Ile Ala Asp
210 215 220
Tyr Arg His Val Arg Leu Ser His Leu Gly Thr Phe Arg Val Phe Gly
225 230 235 240
Ser Thr Lys Ala Leu Val Arg Gln Leu Asp Arg Gly Gly Arg Ile Lys
245 250 255
Ser Cys Thr Val Ser Arg Ser Ala Asp Arg Trp Tyr Val Ser Phe Leu
260 265 270
Val Glu Leu Pro Ile Glu Ile Ala Arg Ser Thr Pro Thr Lys Lys Gln
275 280 285
Tyr Lys Ala Gly Ala Val Gly Ile Asp Leu Gly Val Lys Ser Leu Ala
290 295 300
Ala Leu Ser Thr Gly Glu Ile Ile Pro Asn Pro Arg Phe Leu Arg Thr
305 310 315 320
Ala Asp Lys Lys Ile Lys Lys Leu Gln Arg Lys Ile Ala Arg Cys Gln
325 330 335
Lys Gly Ser Lys Asn Ser Ile Arg Leu Lys Arg Arg Leu Ala Arg Cys
340 345 350
His His Glu Leu Ala Leu Gln Arg Ala Gly Tyr Leu Asn Glu Leu Thr
355 360 365
Ser Met Leu Ala Ser Ser Phe Ser Ala Ile Ala Leu Glu Asp Leu Asn
370 375 380
Val Ala Gly Met Thr Ser Ser Ala Arg Gly Thr Val Glu Asn Pro Gly
385 390 395 400
Lys Asn Val Lys Gln Lys Ala Gly Leu Asn Arg Ser Ile Leu Asp Ile
405 410 415
Ser Pro Gly Arg Ile Arg Thr Leu Leu Glu Tyr Lys Cys Thr Asp Arg
420 425 430
Gly Val Glu Leu Gln Val Ile Asp Arg Phe Phe Pro Ser Ser Gln Leu
435 440 445
Cys Ser Ser Cys Gly Ser Lys Thr Thr Ile Pro Leu Ala Gln Arg Ile
450 455 460
Tyr His Cys Asp Val Cys Gly Gly Val Ile Asp Arg Asp Val Asn Ala
465 470 475 480
Ala Ile Asn Ile Val Tyr Glu Ala Lys Arg Leu Val Glu Gln Lys Cys
485 490 495
Ser Glu His Ser Ala Pro Glu Gly Ala Glu Asp Lys Arg Pro Trp Ser
500 505 510
Val His Leu Pro Ser Thr Gln Tyr Val Asp Gly Leu Tyr Thr Arg Lys
515 520 525
Arg Gln Gly Pro Ser Gly His Ser Ser
530 535
<210> 6
<211> 537
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 6
Met Thr Thr Arg Glu Ile Leu Arg Ala Tyr Arg Val Pro Leu Asp Pro
1 5 10 15
Thr Asp Ala Gln Thr Ala Ala Leu Ala Ser His Ala Gly Ala Ser Arg
20 25 30
Ala Ala Phe Asn Trp Ala Leu Gly Ala Lys Val His Ala His Arg Met
35 40 45
Trp Ser Ala Cys Val Ala Asp Leu Thr Tyr Thr Arg Tyr Gly His Leu
50 55 60
Asp Ala Asp Gln Ala Leu Ala Ala Ala Lys Lys Asp Ala Ser Arg Tyr
65 70 75 80
Tyr Arg Ile Pro Thr Ser Gln Thr Asn Glu Lys Ala Phe Asp Arg Asp
85 90 95
Pro Asp Tyr Ala Trp Arg Thr Glu Val Asn Arg Arg Ser Trp Val Ser
100 105 110
Gly Met Arg Gln Ala Asp Thr Ala Trp Gln Asn Trp Leu Asp Ser Leu
115 120 125
Thr Gly Arg Arg Ala Gly Arg Arg Val Gly Tyr Pro Arg Phe Lys Ser
130 135 140
Lys Gly Arg Cys Arg Asp Ser Phe Thr Leu Ala His Asp Val Lys Arg
145 150 155 160
Pro Ser Ile Arg Pro Asp Gly Tyr Arg Arg Leu Thr Leu Pro Lys Lys
165 170 175
Ile Ser Val Thr Gly Ser Ile Arg Leu Lys Gly Asn Ile Arg His Leu
180 185 190
Ala Arg Arg Ile Arg Arg Gly Val Ala Arg Ile Gln Ser Ala Thr Ile
195 200 205
Ser Arg Ala Gly Asn Gly Trp Ser Val Ser Ile Leu Ala Leu Glu Thr
210 215 220
Leu Asp Ile Pro Asp His Pro Thr Pro Arg Gln Gln Ala Ala Gly Ala
225 230 235 240
Val Gly Val Asp Val Gly Val His His Leu Met Ala Phe Ser Asp Ala
245 250 255
Thr Ile Ile Asp Asn Pro Arg His Leu Arg Ala Ala Gln Lys Arg Leu
260 265 270
Thr Arg Ala Gln Arg Ala Leu Ser Arg Ser Lys Trp Arg Leu Pro Asn
275 280 285
Gly Asp Leu Ile Asp Thr Pro Lys Arg Gly Gln Arg Val Thr Pro Thr
290 295 300
Thr Gly Arg Val Lys Ala Arg Ala Arg Leu Ala Arg Glu His Ala Ala
305 310 315 320
Val Ala Gln His Arg Ala Ser Thr Leu His Ala Ile Thr Lys Gln Leu
325 330 335
Ala Thr Ser His Ala Val Val Ala Val Asp Asp Leu Asn Val Ala Val
340 345 350
Met Thr Arg Ser Ala Arg Gly Ser Ile Asp Lys Pro Gly Arg Asn Val
355 360 365
Ala Ala Lys Ala Gly Leu Asn Arg Ser Ile Leu Asp Ala Ser Phe Ala
370 375 380
Glu Met Arg Arg Gln Leu Thr His Lys Thr Ser Trp Tyr Arg Ser Gln
385 390 395 400
Leu Leu Pro Ser Gly Cys Phe Val Pro Thr Ser Arg Thr Cys Ser Thr
405 410 415
Cys Gly Ala Glu Lys Ala Asn Leu Pro Leu Ser Glu Arg Val Tyr His
420 425 430
Cys Glu Asn Cys Ala Thr Val Leu Asp Arg Asp Val Asn Ala Ala Lys
435 440 445
Asn Val Leu Arg Ala Ala Leu Ala Ser His Asp Ala Pro Gly Met Glu
450 455 460
Glu Ser Gln Asn Ala Arg Gly Gly Arg Gly Glu Thr Ser Val Ala Arg
465 470 475 480
Arg Ser Ala Lys Arg Glu Asp Pro Pro Arg Gly Gly Pro Pro Arg Pro
485 490 495
Cys Asn Arg Val Arg Ser Ser Pro Pro Asp Gly Glu Ala Lys Val Arg
500 505 510
Ala Asn Ala Ser Pro Thr Asn Cys Lys Ala Arg Lys His Ser Thr Pro
515 520 525
Gln Gly Ser Ala Pro Arg Thr Arg Arg
530 535
<210> 7
<211> 492
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 7
Val Ala Gln Val Glu Thr Leu Arg Ala Tyr Arg Phe Ala Leu Asp Pro
1 5 10 15
Thr Ala Ala Gln Leu Ala Asp Leu Asn Arg His Ala Gly Ala Ala Arg
20 25 30
Trp Ala Phe Asn His Ala Leu Gly Val Lys Val Ala Ala His Arg Gln
35 40 45
Trp Arg Ala Gln Val Asp Ala Leu Val Ser Gly Gly Met Pro Glu Ala
50 55 60
Gln Ala Arg Lys Ser Val Lys Val Pro Val Pro Gly Ser Gln Ala Ile
65 70 75 80
Lys Lys Ala Leu Asn Thr Leu Lys Gly Asp Ser Arg Ala Glu Thr Glu
85 90 95
Leu Pro Asp Gly Phe His Gly Leu His Arg Pro Cys Pro Trp Trp His
100 105 110
Glu Val Ser Thr Tyr Ala Phe Gln Ser Ala Phe Ile Asp Ala Asp Arg
115 120 125
Ala Trp Gly Asn Trp Leu Asp Ser Leu Thr Gly Arg Arg Ala Gly Gly
130 135 140
Pro Val Gly Tyr Pro Arg Phe Lys Arg Lys Gly Arg Ala Arg Asp Ser
145 150 155 160
Phe Arg Leu His His Asp Val Lys Lys Pro Thr Ile Arg Leu Asp Gly
165 170 175
Tyr Arg Arg Leu Gln Leu Pro Arg Leu Gly Ser Ile Arg Val His Asp
180 185 190
Ser Gly Lys Arg Leu Ala Arg Leu Val Ala Lys Gly Gln Ala Val Ile
195 200 205
Gln Ser Val Thr Val Ser Arg Gly Gly Asn Arg Trp Tyr Ala Ser Val
210 215 220
Leu Ala Lys Val Val Gln Glu Val Pro Gly Lys Pro Thr Arg Ala Gln
225 230 235 240
Thr Gly Arg Gly Thr Val Gly Val Asp Trp Gly Ile Ser His Leu Ala
245 250 255
Ser Leu Ser Gln Pro Leu Asp Pro Ala Asp Pro Gly Ser Ile His Ile
260 265 270
Ala Asn Pro Arg His Leu Asp Gln Trp Thr Arg Gln Leu Ala Lys Ala
275 280 285
Gln Arg Ala Leu Ser Arg Thr Glu Arg Gly Ser Arg Arg Arg Val Lys
290 295 300
Ala Ala Arg Lys Val Gly Ala Ile Gln His Arg Ile Ala Gln Arg Arg
305 310 315 320
Ala Ser Thr Val His Leu Leu Thr Lys Lys Leu Ala Thr Gly Phe Ala
325 330 335
Thr Val Ala Val Glu Asp Leu Asn Val Arg Gly Met Ser Ala Ser Ala
340 345 350
Arg Gly Thr Val Glu Lys Pro Gly Arg Arg Val Arg Gln Lys Ala Gly
355 360 365
Leu Asn Arg Ala Ile Leu Asp Ala Ala Pro Gly Glu Leu Arg Arg Gln
370 375 380
Leu Thr Tyr Lys Thr Ser Trp Tyr Gly Ser Ala Leu Ala Val Leu Asp
385 390 395 400
Arg Trp His Pro Ser Ser Lys Thr Cys Ser Ser Cys Gly Thr Ala Arg
405 410 415
Pro Lys Leu Thr Leu Ser Glu Arg Val Phe His Cys Thr Thr Cys Gly
420 425 430
Leu Ala Ile Asp Arg Asp His Asn Ala Ala Ile Asn Ile Ala Arg His
435 440 445
Ala Ala Val Pro Leu Val Glu Gly Asp Val Asn Ala Arg Arg Ser Pro
450 455 460
Pro Pro Ala Asp Asn Gly Arg His Gly Arg Ala Ala Arg Gln Lys Arg
465 470 475 480
Glu Gly Pro Pro Pro Gly Gly Pro Pro Arg Arg Glu
485 490
<210> 8
<211> 553
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 8
Met Ser Glu Thr Thr Ala Ser Ser Asp Val Arg Ala Tyr Lys Phe Arg
1 5 10 15
Leu Asp Pro Asn Lys Ala Gln Val Val Arg Leu Ser Gln Cys Ala Gly
20 25 30
Ala Ala Arg Val Gly Tyr Asn Met Leu Ile Ala His Asn Arg Glu Ile
35 40 45
His Gln Glu Ala Arg Arg Arg Arg Asp Ala Leu Ile Ala Ser Gly Val
50 55 60
Asp Pro Ala Ala Ala Asp Ala Lys Leu Arg Glu Gln Arg Arg Thr Asp
65 70 75 80
Pro Ala Val Arg Met Leu Ser Tyr Gln Gln Phe Ser Thr Thr His Leu
85 90 95
Thr Pro Glu Ile Ala Arg His Arg Ala Ala Ser Asp Ala Ile Lys Ala
100 105 110
Gly Ala Asp Pro Ala Glu Val Trp Ala Asp Glu Arg Tyr Ala Glu Pro
115 120 125
Trp Leu His Thr Val Pro Arg Arg Val Leu Val Ser Gly Leu His Asn
130 135 140
Ala Trp Asp Ala Phe Thr Asn Trp Met Thr Ser Phe Thr Gly Gln Arg
145 150 155 160
Ala Gly Arg Arg Val Gly Tyr Pro Arg Phe Lys Arg Lys Gly Arg Ser
165 170 175
Arg Asp Ser Phe Thr Ile Pro Ala Glu Thr Thr Gly Gly Lys Gly Thr
180 185 190
Arg Tyr His His Ser Asp Pro His Lys Gly Val Ile Gly Asp Tyr Lys
195 200 205
His Leu Arg Leu Gly Pro Leu Gly Thr Ile Arg Thr His Gln His Thr
210 215 220
Arg Arg Leu Val Arg Ala Cys Glu His Gly Gly Arg Ile Thr Ser Phe
225 230 235 240
Thr Val Ser Arg Ala Ala Asp Tyr Trp Tyr Val Ser Val Leu Val Glu
245 250 255
Thr Pro Ala Ala Glu Pro Pro Ala Pro Ser Arg Ala Gln Arg Ser Arg
260 265 270
Gly Ala Val Gly Val Asp Val Gly Val His Ser Leu Ala Ala Leu Ser
275 280 285
Thr Gly Glu Leu Ile Ala Asn Pro Arg His Gly Gln Ala Thr Arg Glu
290 295 300
Lys Leu Thr Arg Ala Gln Arg Ala Phe Ala Arg Thr Glu Lys Gly Ser
305 310 315 320
Arg Gly Arg Gln Arg Ala Ala Val Arg Val Ala Arg Ile Gln His Lys
325 330 335
Leu Ala Leu Gln Arg Ala Thr Thr Leu His Lys Leu Thr Lys Ala Leu
340 345 350
Ala Thr Gly Tyr Glu Thr Val Ala Ile Glu Asp Leu Asn Val Ala Gly
355 360 365
Met Thr Arg Ser Ala Lys Gly Thr Val Glu Asn Pro Gly Lys Asn Val
370 375 380
Ala Ala Lys Ala Gly Leu Asn Arg Ser Ile Leu Asp Ala Ala Leu Gly
385 390 395 400
Glu Leu Arg Arg Gln Leu Asp Tyr Lys Thr Arg Trp His Gly Ser Thr
405 410 415
Val Thr Thr Ile Gly Arg Tyr Asp Pro Ser Ser Lys Ala Cys Ser Ser
420 425 430
Cys Gly Thr Val Lys Pro Lys Leu Pro Leu Ser Val Arg Val Tyr Asp
435 440 445
Cys Asp Gln Cys Gly Met Ser Leu Asp Arg Asp Val Asn Ala Ala Arg
450 455 460
Asn Ile Leu Ala Trp Ala Ser Asn Gly Arg Asp Ser Phe Thr Val Pro
465 470 475 480
Gln Thr Glu Val Gly Ala Asp Gly Arg Gly Gly Pro Thr Pro Pro Ser
485 490 495
Pro Arg Gly Glu Gly Thr Leu Val Arg Asp Gln Arg Ser Val Lys Thr
500 505 510
Gly Arg Arg Arg Arg Pro Ala Thr Ser Ala Glu Gln Ser Ala Gly His
515 520 525
Leu Ala Thr Pro Arg Glu Gln Thr Ala Pro Pro Glu Arg Glu Thr Pro
530 535 540
Ser Ser Gly Ala Ser Leu His Val Arg
545 550
<210> 9
<211> 539
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 9
Met Ser Gln Asp Lys Lys Asp Glu Thr Val Asp His Arg Ala Phe Lys
1 5 10 15
Phe Arg Leu Asp Pro Thr Asp Ala Gln Leu Ser Leu Leu Ala Gln Ser
20 25 30
Ala Gly Ala Ala Arg Val Gly Tyr Asn Met Leu Leu Gly His Asn Val
35 40 45
Ala Ala Tyr Gln Ala Arg Gln Glu Leu His Ala Ser Leu Leu Asp Ser
50 55 60
Gly His Asn Glu His Asp Ala Lys Ser Ala Val Thr Asp Arg Lys Thr
65 70 75 80
His Asp Pro Ser Leu Gln Thr Leu Ser Tyr Gln Ser Phe Ser Thr His
85 90 95
Tyr Leu Thr Pro Glu Ile Thr Arg His Arg Ile Ala Ser Asp Ala Ile
100 105 110
Lys Ala Gly Ala Asp Pro Ser Lys Val Trp Asn Asp Pro Arg Tyr Glu
115 120 125
Thr Pro Trp Met His Thr Ile Pro Arg Arg Val Phe Ile Ser Gly Leu
130 135 140
Gln His Ala Asn Thr Ala Tyr Thr Asn Trp Phe Ala Ser Met Arg Gly
145 150 155 160
Asp Arg Ala Gly Ala Arg Val Gly Arg Pro Arg Phe Lys Lys Lys Gly
165 170 175
Arg Cys Arg Asp Ser Phe Thr Ile Pro Ala Pro Glu Ala Met Gly Ala
180 185 190
Lys Gly Ala Pro Tyr Lys Arg Gly Glu Pro Arg Ser Gly Val Ile Glu
195 200 205
Asp Tyr Arg His Val Arg Leu Ala Phe Leu Gly Val Leu Arg Thr His
210 215 220
Asn Ser Thr Lys Arg Leu Val Arg Ala Cys Gln Arg Gly Gly Lys Ile
225 230 235 240
Lys Ser Phe Thr Val Ser Arg Asn Ala Asp Arg Trp Tyr Val Ser Phe
245 250 255
Leu Val Ala Ser Pro Ala Arg Ala Ala Val Thr Thr Lys Arg Gln Arg
260 265 270
Ala Asn Gly Ala Val Gly Val Asp Val Gly Val His Ser Leu Ala Ala
275 280 285
Leu Ser Asn Gly Glu Val Phe Ser Asn Pro Arg His Gly Gln Leu Ala
290 295 300
Gln Lys Lys Leu Lys Arg Ala Gln Arg Lys Leu Ala Arg Thr Gln Lys
305 310 315 320
Gly Ser Lys Gly Arg Ala Arg Ala Ala Gln Arg Val Gly Arg Leu Gln
325 330 335
His Leu Val Thr Leu Gln Arg Glu Thr Thr Ala His His Leu Thr Lys
340 345 350
Tyr Leu Ala Thr His Tyr Cys Ala Val Ala Ile Glu Asp Leu Asn Val
355 360 365
Ser Gly Met Thr Arg Ser Ala Lys Gly Thr Met Glu His Pro Gly Lys
370 375 380
Asn Val Lys Ala Lys Ser Gly Leu Asn Arg Ser Ile Leu Asp Ala Ser
385 390 395 400
Phe Gly Arg Ile His Gln Gln Leu Arg Tyr Lys Met Gly Trp Ser Gly
405 410 415
Ala Asp Leu Gln Ile Ile Gly Arg Phe Val Pro Ser Ser Lys Met Cys
420 425 430
Ser Glu Cys Gly Thr Val Lys Ser Lys Leu Pro Leu Ser Glu Arg Val
435 440 445
Tyr Glu Cys Glu His Cys Gly Leu Lys Ile Asp Arg Asp Val Asn Ala
450 455 460
Ala Ile Asn Ile Leu His Ala Gly Ala Gly Ser Ile Thr Pro Ser Asn
465 470 475 480
Ser Pro Leu Thr Arg Gly Ser Leu Asn Gly Arg Gly Asp Gln Thr Cys
485 490 495
Leu Ser Pro Ser Gly Glu Arg Ile Thr Arg Gly Asp Ser Arg Ser Val
500 505 510
Lys Ala Asp Pro Ile Trp Cys Arg Ser Pro Gln Thr Ser Asn Arg Cys
515 520 525
Ser Ser Leu Thr Val Arg Gly Thr Ala Leu Lys
530 535
<210> 10
<211> 552
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 10
Met Ser Val Ala Thr Asp Ala Pro Thr Gln Arg Leu Arg Ala Phe Lys
1 5 10 15
His Arg Leu Asp Pro Asn Ala Ser Gln Leu Glu Leu Leu Gly Gln Tyr
20 25 30
Ala Gly Ala Ala Arg Val Ala Phe Asn Met Leu Thr Ala His Asn Arg
35 40 45
Ala Ala Leu Gln Ala Gly Trp Asp Arg Arg Arg Gln Leu Ala Glu Ala
50 55 60
Gly Val Pro Ala Glu Glu Leu Val Gly Arg Met Lys Ala Glu Arg Ala
65 70 75 80
Ala Asp Pro Ala Leu Lys Val Ala Gly Tyr Gln Gln Phe Ala Thr Ala
85 90 95
His Leu Thr Pro Met Val Arg Ala His Arg Glu Ala Ala Glu Ala Ile
100 105 110
Ala Ala Gly Ala Asp Pro Gly Gln Val Trp Ala Asp Glu Arg Tyr Ala
115 120 125
Gln Pro Trp Met His Thr Val Pro Arg Arg Val Leu Val Ser Gly Leu
130 135 140
Gln Asn Ala Ala Lys Ala Thr Glu Asn Trp Met Ala Ser Ala Cys Gly
145 150 155 160
Ala Arg Ala Gly Arg Arg Val Gly Leu Pro Arg Phe Lys Lys Lys Gly
165 170 175
Arg Ser Arg Asp Ser Phe Thr Ile Pro Ala Pro Glu Val Ile Gly Ala
180 185 190
Ala Gly Thr Ala Tyr Lys Arg Gly Glu Ala Arg Gly Gly Val Ile Thr
195 200 205
Asp His Arg His Leu Arg Leu Ala Ser Leu Gly Thr Ile Arg Thr Met
210 215 220
Asp Lys Thr Thr Arg Leu Val Arg Ser Cys Arg Arg Gly Ala Val Val
225 230 235 240
Arg Ser Val Thr Ile Ser Gln Ala Gly Gly His Trp Tyr Ala Ser Val
245 250 255
Leu Val Ala Glu Pro Val Thr Leu Arg Arg Gly Pro Ser Arg Arg Gln
260 265 270
Arg Ala Asn Gly Val Val Gly Val Asp Leu Gly Val Lys His Leu Ala
275 280 285
Ala Leu Ser Thr Gly Glu Leu Ile Pro Asn Ala Arg Ala Gly Gln Ala
290 295 300
Gln Ala Arg Arg Leu Ala Arg Leu Gln Arg Ala Leu Ala Arg Thr Glu
305 310 315 320
Arg Gly Ser Arg Arg Arg Glu Arg Val Arg Arg Gln Ile Ser Ala Leu
325 330 335
His His Gln Val Ala Leu Arg Arg Thr Gly Met Leu His Glu Val Ser
340 345 350
Thr Arg Leu Ala Arg Asp Tyr Ala Ile Val Ala Leu Glu Asp Leu Asn
355 360 365
Val Ala Gly Met Thr Gly Ser Ala Ala Gly Thr Ile Glu His Pro Gly
370 375 380
Lys Asn Val Ala Ala Lys Ala Gly Leu Asn Arg Ala Ile Leu Asp Ala
385 390 395 400
Gly Leu Gly Thr Leu Arg Ala Gln Leu His Tyr Lys Thr Ser Trp Ala
405 410 415
Gly Ser Gln Val Lys Met Ile Asp Arg Phe Ala Pro Ser Ser Lys Thr
420 425 430
Cys Ser Arg Cys Gly Ala Val Lys Ala Thr Leu Ser Leu Arg Glu Arg
435 440 445
Val Tyr Glu Cys Glu Val Cys Ala Leu Val Ile Asp Arg Asp Val Asn
450 455 460
Ala Ala Ile Asn Ile Arg Ala Trp Ala Val Gln Glu Gly Thr Gly His
465 470 475 480
Arg Val Glu Leu Ala Gln Gly Asn Gly Glu Ser Gln Asn Gly Arg Arg
485 490 495
Ala Ala Gly Arg Thr Pro Pro Ser Gly Gly Pro Thr Gly Trp Gln Arg
500 505 510
Arg Ser Val Lys Pro Ala Pro Arg Gly Ala Gly Arg Ser Ser Arg Ala
515 520 525
Thr Gly Trp Ser Ser His Thr Pro His Ile Glu Arg Glu His Ala Glu
530 535 540
Ala Asp Val Phe Gly Pro Val Arg
545 550
<210> 11
<211> 485
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 11
Met Val Arg Ser Val Gly Asp Asp Leu Arg Ala Tyr Arg Phe Ala Leu
1 5 10 15
Asp Leu Arg Pro Ala Gln Leu Arg Ala Val Ala Glu His Ala Gly Ala
20 25 30
Ala Arg Trp Ala Tyr Asn His Ala Leu Gly Val Lys Phe Ala Ala Leu
35 40 45
Lys Gln Arg Gln Thr Val Ile Ala Glu Leu Val Thr Ala Gly Val Asp
50 55 60
Ala Gln Ala Ala Ala Arg Arg Ala Pro Arg Ile Pro Thr Arg Pro Gln
65 70 75 80
Ile Gln Lys Asp Leu Asn Ala Thr Lys Gly Asp Ser Arg Ser Gly Val
85 90 95
Asp Gly Leu Cys Pro Trp Trp Trp Thr Ala Ser Thr Tyr Ala Phe Gln
100 105 110
Ser Ala Met Ile Asp Ala Asp Arg Ala Trp His Asn Trp Met Ser Ser
115 120 125
Val Thr Gly Gln Arg Ala Gly Arg Arg Val Gly Arg Pro Arg Phe Lys
130 135 140
Ser Lys His Arg Cys Arg Asp Ser Phe Arg Ile His His Asn Val Lys
145 150 155 160
Gln Pro Thr Ile Arg Pro Asp Thr Ser Gly Tyr Arg Arg Leu Ile Val
165 170 175
Pro Arg Leu Gly Ser Leu Arg Thr His Asp Ser Thr Lys Arg Leu Arg
180 185 190
Arg Ala Leu Asp Arg Gly Ala Val Ile Gln Ser Val Thr Ile Ser Arg
195 200 205
Gly Gly His Arg Trp Tyr Ala Ser Val Leu Val Lys Thr Pro Asp Ala
210 215 220
Pro Ala Val Gly Pro Ser Arg Ala Gln Arg Lys Ala Gly Ile Val Gly
225 230 235 240
Val Asp Ile Gly Val His Ser Leu Ala Ala Leu Ser Thr Gly Glu Leu
245 250 255
Val Val Asn Pro Arg His Leu Asn Ser Ser Cys Asp Arg Leu Gly Ala
260 265 270
Ala Gln Arg Ala Leu Ala Arg Cys Gln Lys Gly Ser Asn Arg Arg Arg
275 280 285
Arg Ala Ala Arg Leu Leu Gly Arg Arg His His Glu Leu Ala Glu Arg
290 295 300
Arg Ala Thr Ala Leu His Gln Leu Thr Lys Arg Leu Ala Thr Cys Trp
305 310 315 320
Ser Val Val Ala Val Glu Asp Leu His Val Ala Gly Met Ile Arg Ser
325 330 335
Ala Arg Gly Thr Val Asp Lys Pro Gly Arg Asn Val Arg Ala Lys Ser
340 345 350
Ala Leu Asn Arg Ala Ile Leu Asp Val Ala Pro Gly Glu Leu Arg Arg
355 360 365
Gln Leu Ala Tyr Lys Thr Val Trp Tyr Gly Ser Ser Leu Val Val Cys
370 375 380
Asp Arg Phe Tyr Pro Ser Thr Gln Thr Cys Ser Ala Cys Gly Ala Lys
385 390 395 400
Ala Lys Leu Thr Leu Ala Asp Arg Val Phe Arg Cys Thr Ala Cys Gly
405 410 415
Phe Gly Pro Thr Asp Arg Asp Ile Asn Ala Ala Arg Asn Ile Ala Ala
420 425 430
Asn Ala Ala Val Ala Ser Gly Ala Gly Glu Thr Leu Asn Ala Arg Arg
435 440 445
Ala Asp Gln Gln His Leu Pro Gln Val Gly Pro Cys Arg Arg Pro Ala
450 455 460
Met Lys Arg Glu Gly His His Gln Ser Lys Thr Asp Ser Gly His Leu
465 470 475 480
Ser Arg Ala Thr Gly
485
<210> 12
<211> 569
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 12
Met Pro Leu Asn Gly Trp Thr Met Leu Ala Gln Glu Glu Val Arg Ala
1 5 10 15
Met Thr Thr Thr Thr Gly Thr Asp Leu Ala Pro Arg Leu Arg Ala Phe
20 25 30
Lys His Arg Leu Asp Pro Asn Pro Ala Gln Ala Thr Leu Leu Ala Gln
35 40 45
Tyr Ala Gly Ala Ala Arg Val Ala Tyr Asn Met Leu Ile Ala His Asn
50 55 60
Arg Ala Ala Leu Ala Ala Gly Ala Ala Arg Arg Thr Glu Leu Ala Glu
65 70 75 80
Ser Gly Leu Ala Gly Pro Glu Leu Ala Ala Arg Met Lys Ala Glu Arg
85 90 95
Ala Ala Asp Pro Thr Leu Arg Val Ala Ser Tyr Gln Ser Tyr Ser Thr
100 105 110
Ala His Leu Thr Pro Leu Ile Arg Arg His Arg Glu Ala Ala Ala Ala
115 120 125
Ile Ala Ala Gly Ala Asp Pro Ala Glu Ala Trp Thr Asp Glu Arg Tyr
130 135 140
Ala Glu Pro Trp Met His Thr Val Pro Arg Arg Val Leu Val Ser Gly
145 150 155 160
Leu Gln Asn Ala Ala Lys Ala Thr Glu Asn Trp Met Ala Ser Ala Ser
165 170 175
Gly Thr Arg Ala Gly Ala Arg Val Gly Leu Pro Arg Phe Lys Lys Lys
180 185 190
Gly Arg Ser Arg Asp Ser Phe Thr Ile Pro Ala Pro Glu Val Ile Gly
195 200 205
Ala Ala Gly Thr Pro Tyr Lys Arg Gly Glu His Arg Arg Gly Val Ile
210 215 220
Thr Asp His Arg His Leu Arg Leu Ala Ser Leu Gly Thr Ile Arg Thr
225 230 235 240
Tyr Asp Lys Thr Ser Arg Leu Val Arg Ala Cys Arg Arg Gly Ala Gln
245 250 255
Ile Arg Ser Met Thr Ile Ser Gln Ala Gly Gly Arg Trp Tyr Ala Ser
260 265 270
Ile Leu Val Ala Asp Pro Thr Pro Ile Arg Thr Gly Pro Ser Arg Arg
275 280 285
Gln Arg Ala Asn Gly Ala Val Gly Val Asp Leu Gly Val Lys His Leu
290 295 300
Ala Ala Leu Ser Thr Gly Glu Val Ile Asp Asn Gly Arg Pro Gly Ala
305 310 315 320
Arg Gln Ala Ala Arg Leu Thr Arg Leu Gln Arg Ala Tyr Ala Arg Thr
325 330 335
Gln Pro Gly Ser Asn Arg Arg Glu Arg Val Arg Arg Gln Ile Ala Ala
340 345 350
Leu His His Gly Ile Ala Leu Arg Arg Ala Gly Leu Leu His Gln Val
355 360 365
Ser Thr Arg Leu Ala Met Asp Phe Ala Val Val Ala Leu Glu Asp Leu
370 375 380
Asn Val Ala Gly Met Thr Arg Ser Ala Arg Gly Thr Leu Glu Ala Pro
385 390 395 400
Gly Arg Asn Val Ala Ala Lys Ser Gly Leu Asn Arg Ala Ile Leu Asp
405 410 415
Ala Gly Leu Gly Met Leu Arg Arg Gln Leu Asp Tyr Lys Thr Ser Trp
420 425 430
Ala Gly Ser Gln Val Lys Met Ile Asp Arg Phe Ala Pro Ser Ser Lys
435 440 445
Ala Cys Ser Arg Cys Gly Thr Val Lys Ser Thr Leu Ser Leu Ala Glu
450 455 460
Arg Thr Phe Glu Cys Glu Ala Cys His Leu Val Ile Asp Arg Asp Val
465 470 475 480
Asn Ala Ala Ile Asn Ile Arg Ala Trp Ala Val Gln Glu Glu Arg Arg
485 490 495
Ala Gly Val Glu Leu Ala Arg Gly Arg Arg Glu Ser Arg Asn Gly Arg
500 505 510
Gly Ala Ala Val Ser Gly Pro Pro Ser Gly Gly Ala Ala Gly Gln Gly
515 520 525
Arg Gly Ser Val Lys Pro Ala Pro Gln Gly Val Gly Met Ser Ser Arg
530 535 540
Ala Thr Gly Trp Ser Ser Gln Pro Pro Ser Thr Glu Gly Glu Ser Ala
545 550 555 560
Glu Arg Gly Ala Ser Ala Leu Ala Arg
565
<210> 13
<211> 505
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 13
Met Gly Glu Gln Leu Arg Ala Tyr Arg Tyr Ala Leu Asp Leu Thr Asp
1 5 10 15
Ala Gln Ala Ser Val Val Ala Gln His Ala Gly Ala Ala Arg Trp Ala
20 25 30
Tyr Asn His Ala Ile Ala Thr Lys Phe Lys Ala Leu Asp Glu Arg Gln
35 40 45
Ala Ala Ile Arg Glu Ala Val Asp Ala Gly Val Asp Pro Ala Val Ala
50 55 60
Ala Asn Leu Ala Pro Lys Ile Pro Ser Lys Phe Asp Ile Gln Lys Ala
65 70 75 80
Leu Asn Lys Val Lys Gly Asp Asp Arg Thr Gly Arg Glu Gly Glu Cys
85 90 95
Pro Trp Trp His Ser Val Ser Thr Phe Ala Phe Gln Ser Ala Phe Ser
100 105 110
Asp Ala Asp Arg Ala Trp Lys Asn Trp Leu Asp Ser Ile Thr Gly Asn
115 120 125
Arg Lys Gly Arg Arg Val Gly Arg Pro Arg Phe Lys Ala Lys Arg Arg
130 135 140
Ser His Asp Ser Phe Arg Ile His His Asp Val Lys Asn Pro Thr Ile
145 150 155 160
Arg Pro Asp Val Gly Cys Tyr Arg Arg Ile Arg Val Pro Arg Leu Gly
165 170 175
Ser Leu Arg Val His Ser Ser Thr Lys Gln Leu Cys Arg Ala Leu Asp
180 185 190
Arg Gly Ala Val Ile Gln Ser Val Thr Ile Ser Arg Gly Gly His Arg
195 200 205
Trp Tyr Ala Ser Ile Leu Thr Lys Gln Glu Ile Val Ser Glu Val Leu
210 215 220
Pro Thr Arg Arg Gln Arg Leu Ala Gly Thr Val Gly Val Asp Leu Gly
225 230 235 240
Val His Thr Leu Ala Ala Leu Ser Thr Gly Glu Thr Val Ser Asn Pro
245 250 255
Arg His Ala Asn Met Ala Arg Thr Arg Leu Thr Lys Ala Gln Gly Ala
260 265 270
Leu Ser Arg Thr Lys Lys Gly Ser Asn Arg Arg Arg Arg Ala Ala Arg
275 280 285
Leu Val Gly Arg Arg His His Glu Val Ala Glu Arg Arg Ala Ser Thr
290 295 300
Leu His Ala Val Thr Lys Ala Leu Ala Thr Arg Trp Ala Thr Val Ala
305 310 315 320
Val Glu Asp Leu Asn Val Ala Gly Met Thr Ala Ser Ala Arg Gly Thr
325 330 335
Val Asp Lys Pro Gly Arg Asn Val Arg Ala Lys Ala Gly Leu Asn Arg
340 345 350
Ala Ile Leu Asp Val Ser Pro Gly Glu Phe Arg Arg Gln Leu Glu Tyr
355 360 365
Lys Thr Ala Trp Tyr Gly Ser Ala Leu Ala Val Ile Asp Arg Phe Tyr
370 375 380
Pro Ser Ser Gln Thr Cys Ser Ala Cys Gly Ala Arg Ala Lys Leu Thr
385 390 395 400
Leu Ala Glu Arg Ile Tyr Arg Cys Ala Ala Cys Gly Phe Thr Ala Asp
405 410 415
Arg Asp Val Asn Ala Ala Ile Asn Ile Ala Ala Gln Ala Ala Val Ala
420 425 430
Pro Gly Lys Gly Glu Thr Ile Asn Ala Arg Arg Ala Val Ser Asp His
435 440 445
Pro Gln Pro Ala Gly Thr Arg Ser Leu Thr Ala Met Lys Arg Glu Gly
450 455 460
His Arg Asp Asn Ala Val Val Thr Pro Thr Arg Gln Arg Val Gly His
465 470 475 480
Pro Ile Arg Cys Gly Met Arg Leu Asp Glu Arg Ile Gly Ala Gly Gln
485 490 495
Glu Ala Ser Ala Pro His Ala Tyr Gly
500 505
<210> 14
<211> 561
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 14
Met Lys Glu Leu Ala Ala Asp Gly Ile Pro Val Ala Val Thr Cys Arg
1 5 10 15
Val Leu Lys Leu Ala Arg Gln Pro Tyr Tyr Arg Trp Leu Ala Gln Pro
20 25 30
Val Thr Asp Ala Glu Leu Val Glu Ala His Arg Ala Asn Ala Leu Phe
35 40 45
Asp Ala His Arg Asp Asp Pro Glu Phe Gly Tyr Arg Tyr Leu Ala Glu
50 55 60
Glu Ala Arg Glu Ala Gly Glu Pro Met Ala Glu Arg Thr Ala Trp Arg
65 70 75 80
Ile Cys Ser Gln Asn Arg Leu Trp Ser Val Phe Gly Lys Arg Lys Arg
85 90 95
Gly Lys Asn Gly Lys Thr Gly Pro Pro Val His Asp Asp Leu Val Glu
100 105 110
Arg Asp Phe Thr Ala Glu Ala Pro Asn Gln Leu Trp Leu Ala Asp Ile
115 120 125
Thr Glu His Arg Thr Gly Glu Gly Lys Leu Tyr Leu Cys Ala Ile Lys
130 135 140
Asp Ala Phe Ser Asn Arg Ile Val Gly Tyr Ser Ile Asp Ser Arg Met
145 150 155 160
Lys Ser Arg Leu Ala Thr Thr Ala Leu Ser Ser Ala Val Ala Arg Arg
165 170 175
Gly Asp Val Ala Gly Cys Ile Leu His Ser Asp Arg Gly Ser Gln Phe
180 185 190
Arg Ser Arg Arg Phe Val Gln Ala Leu Asn His His Ala Met Val Gly
195 200 205
Ser Met Gly Arg Val Gly Ala Ala Gly Asp Asn Ala Ala Met Glu Ser
210 215 220
Phe Phe Ser Leu Leu Gln Lys Asn Val Leu Asp Arg Arg Arg Trp Asp
225 230 235 240
Thr Arg Glu Gln Leu Arg Ile Ala Ile Val Thr Trp Ile Glu Arg Thr
245 250 255
Tyr His Arg Arg Arg Arg Gln Ala Gly Pro Arg Thr Ile Asp Pro His
260 265 270
Arg Ile Arg Ser Asp Tyr Glu Arg Thr Gly Gln Ser Gly Arg Val Thr
275 280 285
Glu Thr Val Thr Tyr Ser Cys Ser Arg Pro Ala Gly Arg Val Gly Val
290 295 300
Asp Leu Gly Val His His Leu Ala Ala Leu Ser Thr Gly Glu Leu Ile
305 310 315 320
Asp Asn Pro Arg His His Arg Gln Ala Arg Arg Ala Leu Thr Lys Ala
325 330 335
Gln Gln Ala Leu Ser Arg Thr Glu Lys Gly Ser Gln Arg Arg Arg Lys
340 345 350
Ala Cys Ala Thr Leu Ala Arg Arg His His Leu Leu Ala Glu Arg Arg
355 360 365
Ala Thr Thr Val His Gly Ile Thr Lys Arg Leu Ala Thr Gly Trp Ser
370 375 380
Glu Val Ala Ile Glu Asp Leu Asn Val Ala Gly Met Thr Arg Ser Ala
385 390 395 400
Arg Gly Thr Val Glu Ser Pro Gly Val Asn Val Ala Ala Lys Ala Gly
405 410 415
Leu Asn Arg Ser Ile Leu Asp Ala Ala Pro Asp Glu Leu Arg Arg Gln
420 425 430
Leu Thr Tyr Lys Thr Gln Trp Tyr Gly Ser Arg Leu Ala Val Cys Asp
435 440 445
Arg Trp Phe Pro Ser Thr Gln Ala Cys Ser Ala Cys Gly Ala Lys Ala
450 455 460
Lys Leu Thr Leu Ala Asp Arg Val Phe His Cys Pro Ala Cys Gly Phe
465 470 475 480
Gly Pro Ile Asn Arg Asp His Asn Ala Ala Arg Asn Ile Ala Ala His
485 490 495
Ala Val Ile Val Ala Pro Gly Thr Gly Glu Thr Glu Asn Ala Arg Arg
500 505 510
Ala Asp Ala Gly Pro Pro Pro Arg Val Glu Thr Thr Pro Pro Ser Val
515 520 525
Met Lys Arg Glu Asp His Pro Thr Arg Val Ala Thr Ser Ala Glu Gln
530 535 540
Ser Ala Asp His Pro Lys Pro Ala Asp Gln His Thr Leu Pro Leu Val
545 550 555 560
Asn
<210> 15
<211> 496
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 15
Met Ala Gln Val Leu Arg Ala Phe Arg Tyr Ala Leu Asp Pro Ser Pro
1 5 10 15
Pro Gln Leu Glu Thr Leu Gln Arg Cys Ala Gly Asn Ala Arg Leu Ala
20 25 30
Phe Asn Phe Ala Leu Ala Met Lys Lys Asp Ala His Gln Arg Trp Arg
35 40 45
Asp Glu Val Asp Ile Leu Ile Thr Thr Gly Leu Ser Glu Lys Asp Ala
50 55 60
Arg Thr Arg Leu Lys Gly Thr Ser Lys Ile Pro Asn Lys Pro Asp Val
65 70 75 80
Tyr Lys Ala Phe Gln His Leu Arg Gly Asp Ala Ala Gln Gly Ile Asp
85 90 95
Gly Ile Ala Pro Trp His Ala Glu Ile Pro Thr Tyr Val Phe Gln Ser
100 105 110
Ala Phe Gln Asp Ala Asp Arg Ala Trp Lys Asn Trp Leu Asp Ser Tyr
115 120 125
Thr Gly Lys Arg Ala Gly Arg Arg Val Gly Tyr Pro Arg Phe Lys Lys
130 135 140
Lys Phe Arg Ser Arg Asp Ser Phe Arg Leu His His Asp Ala Lys Lys
145 150 155 160
Pro Lys Pro Ala Leu Arg Leu Glu Gly Tyr Arg Arg Leu Arg Leu Gly
165 170 175
Gly Ala Leu Lys Thr Val Arg Leu His Gly Ser Ala Lys Pro Leu His
180 185 190
Arg Leu Val Ser Ser Gly Arg Ala Val Val Gln Ser Val Thr Val Ser
195 200 205
Arg Gly Gly Thr Arg Trp Tyr Ala Ser Val Leu Cys Lys Val Glu Thr
210 215 220
Asp Val Pro Asp Pro Thr His Arg Gln Lys Ala Asn Gly Arg Ile Gly
225 230 235 240
Leu Asp Trp Gly Leu Asn His Leu Ala Ala Leu Ser Thr Pro Leu Asp
245 250 255
Gly His His Leu Val Asp Asn Pro Arg His Leu Arg His Ala Ser Lys
260 265 270
Arg Leu Thr Lys Ala Gln Arg Ala Leu Ser Arg Thr Gln Lys Gly Ser
275 280 285
Ala Arg Arg Arg Arg Ala Ala Ala Arg Val Gly Lys Leu His His Leu
290 295 300
Val Ala Glu Gln Arg Ala Thr Phe Leu His Thr Leu Thr Lys Arg Leu
305 310 315 320
Thr Thr Thr Phe Ala Cys Val Ala Ile Glu Asp Leu Asn Val Ala Gly
325 330 335
Met Thr Arg Ser Ala Arg Gly Thr Val Gln Leu Pro Gly Lys Asn Val
340 345 350
Ser Ser Lys Ala Gly Leu Asn Arg Ser Ile Leu Asp Ala Ala Pro Ala
355 360 365
Glu Leu Arg Arg Gln Leu Glu Tyr Lys Thr Ser Trp Tyr Gly Ser His
370 375 380
Ile Ala Ile Leu Asp Arg Trp Phe Pro Ser Ser Lys Thr Cys Ser Gly
385 390 395 400
Cys Gly Trp Arg Asn Pro Ser Leu Pro Leu Ser Glu Arg Glu Phe Val
405 410 415
Cys Ala Glu Cys Gly Leu Arg Leu Asp Arg Asp Leu Asn Ala Ala Arg
420 425 430
Asn Ile Ala Ala His Ala Glu Val Pro Ala Ser Gly Thr Gly Ala Pro
435 440 445
Gly Arg Gly Glu Ser Val Asn Ala Arg Gly Gly Cys Val Ser Pro Arg
450 455 460
Leu Leu Arg Glu Ser Gly Gln His Pro Ser Lys Arg Glu Asp Ala Ala
465 470 475 480
Pro Ser Gly Pro Ala Pro Pro Arg Arg Ser Asn Pro Pro Thr Phe Pro
485 490 495
<210> 16
<211> 499
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 16
Met Ala Glu Val Leu Arg Thr Phe Lys Phe Ala Leu Asp Pro Thr Ser
1 5 10 15
Thr Gln Leu Gln Thr Leu Gly Arg Tyr Ser Gly Asn Ala Arg Leu Ala
20 25 30
Tyr Asn Phe Ala Leu Ala Met Lys Lys Glu Ala His Gln Gln Trp Arg
35 40 45
Asp Arg Val Asp Thr Leu Val Ala Thr Gly Val Asp Glu Lys Ala Ala
50 55 60
Arg Asp Arg Leu Lys Gly Thr Val Lys Val Pro Lys Lys Pro Gln Val
65 70 75 80
Tyr Lys Ala Phe Gln Leu Leu Arg Gly Asp Ala Ala Lys Gly Ile Glu
85 90 95
Gly Leu Ala Pro Trp His Ala Glu Ile Pro Thr His Val Phe Gln Ser
100 105 110
Ala Trp Ile Asn Ala Asp Arg Ala Trp Gln Asn Trp Ile Asp Ser Tyr
115 120 125
Arg Gly Thr Arg Ala Gly Arg Arg Val Gly Tyr Pro Lys Phe Lys Lys
130 135 140
Lys Phe Arg Ser Arg Asp Ser Phe Arg Leu His Gln Ser Ser Gly Arg
145 150 155 160
Pro Val Leu Arg Leu Glu Gly Tyr Arg Arg Leu His Leu Ser Gly Gln
165 170 175
Leu Gly Thr Val Arg Leu His Gly Ser Ala Lys Arg Leu His Arg Leu
180 185 190
Val Ser Ser Ser Gln Ala Lys Val Gln Ser Val Thr Ile Ser Arg Gly
195 200 205
Gly Ser Arg Trp Tyr Ala Asn Val Leu Cys Lys Val Asp Ser Asn Leu
210 215 220
Pro Gly Pro Thr Arg Arg Gln Arg Ala Asn Asn Arg Val Gly Leu Asp
225 230 235 240
Trp Gly Val His His Leu Ala Ala Leu Ser Thr Pro Ile Glu Gly Thr
245 250 255
Thr Leu Val Asp Asn Pro Lys His Leu Ala Lys Ala Thr Thr Arg Leu
260 265 270
Arg Lys Ala Gln Arg Ala Leu Ser Arg Thr Gln Lys Gly Ser Lys Arg
275 280 285
Arg His Thr Ala Ala Ser Arg Val Gly Lys Leu His His Gln Val Ala
290 295 300
Glu Gln Arg Ala Thr Phe Leu His Ala Leu Thr Lys Arg Leu Cys Thr
305 310 315 320
Thr Phe Ala Phe Val Ala Val Glu Asp Leu Asn Val Ala Gly Met Thr
325 330 335
His Ser Ala Arg Gly Thr Lys Asp Lys Pro Gly Thr Asn Val Arg Ala
340 345 350
Lys Ala Gly Leu Asn Arg Ser Ile Leu Asp Ala Ala Pro Gly Glu Leu
355 360 365
Arg Arg Gln Leu Glu Tyr Lys Thr Ser Trp Tyr Gly Ser Gln Leu Ala
370 375 380
Leu Cys Asp Arg Trp Phe Pro Ser Ser Lys Thr Cys Ser Ala Cys Gly
385 390 395 400
Trp Gln Asn Pro Ser Leu Ser Leu Ser Glu Arg Val Phe Val Cys Ala
405 410 415
Glu Cys Gly Leu Arg Val Asp Arg Asp Leu Asn Ala Ala Arg Asn Ile
420 425 430
Ala Ala His Ala Glu Val Pro Ser Gly Thr Gly Ala Pro Gly Arg Gly
435 440 445
Glu Ser Val Asn Ala Arg Gly Gly Arg Val Ser Pro Pro Leu Ser Arg
450 455 460
Glu Arg Gly Gln Arg Pro Ser Lys Arg Glu Asp Ala Gly Leu Lys Gly
465 470 475 480
Pro Ala Pro Pro Arg Arg Ser Asn Pro Pro Thr Ser Leu Thr Arg Val
485 490 495
Ser Arg Thr
<210> 17
<211> 560
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 17
Met Leu Ile Ala His Lys Ile Lys Leu Leu Pro Thr Pro Glu Gln Ala
1 5 10 15
Ala Leu Phe Arg Arg Cys Glu Thr Val Ala Lys Ala Thr Tyr Asn Gln
20 25 30
Ala Leu Arg Met Trp Gln Glu Asp Tyr Gln Arg Tyr Leu Arg Glu Met
35 40 45
Val Ala Leu Cys Gly Ser Arg Gly Ile Ser Ala Leu Thr Asp Ser Pro
50 55 60
Arg Cys Leu Trp Pro Arg Phe Lys Lys Arg Ser Asp Leu Glu Lys Glu
65 70 75 80
Leu Ile Ala Ala Gly Val Ala Lys Asp Asp Ile Pro Ala Thr Ser Gly
85 90 95
Phe Arg Trp Ile Ser Lys His Tyr Ala Asn Arg Pro Ala His Trp Glu
100 105 110
Asp Leu Gly Tyr Trp Pro Gly Ala Gly Lys Ile Thr Asn Leu Lys Asp
115 120 125
Ala Ala Asn His Phe Phe Lys Asp Gly Phe Gly Tyr Pro Arg Pro Ile
130 135 140
Lys Glu Asp Asp Val Cys Gly Phe Leu Val Leu Ser Ser Ala Glu Lys
145 150 155 160
Cys Gly Gly Lys Lys Asp Ala Asn Gly Arg His Asn Gly Pro Phe Ala
165 170 175
Arg His Val Asp Arg Pro Val Trp Gln Arg Pro Leu Glu Phe Asn Val
180 185 190
Pro Lys Ile Gly Lys Val Arg Met Ala Glu Pro Leu Arg Phe Glu Lys
195 200 205
Phe Glu Thr Gln Lys Lys Ile Ser Pro Arg Gly Lys Asn Glu Gly Gln
210 215 220
Glu Ile Glu Thr Phe Pro Val Ala Ser Val Thr Ile Ser Arg Asn Lys
225 230 235 240
Ala Gly Asp Trp Phe Ala Ser Ile Leu Cys Ser Val Pro Glu Ile Glu
245 250 255
Pro Asn Ala Pro Val Arg Asp Tyr Lys Val Gly Val Asp Leu Gly Ile
260 265 270
Lys Thr Leu Ala Lys Leu Ser Asp Glu Val Pro Gly Gln Pro Asp Arg
275 280 285
Phe Pro Ala Arg Lys Ala Leu Ser Asn Ala Glu Arg Arg Leu Leu Arg
290 295 300
Leu Glu Arg Lys Met Ser Arg Gln Gln Ala Gln Ala Ala Arg Arg Lys
305 310 315 320
Arg Lys Leu Ser Glu Cys Lys Asn Tyr Gln Lys Thr Lys Arg Leu His
325 330 335
Ala Leu Ala Trp Lys Lys Val Gly His Arg Arg Glu Ser Tyr Ser His
340 345 350
Gln Leu Thr Thr Val Leu Val Arg Asp Tyr Pro Val Ile Gly Ile Glu
355 360 365
Asp Leu Gly Val Lys Gly Met Met Arg Lys Gly Lys Pro Gly Asn Lys
370 375 380
Pro Lys Lys Ala Arg Leu Asn Arg Ala Ile Gly Asp Ala Gly Phe Gly
385 390 395 400
Arg Phe Arg Ile Gln Leu Glu Tyr Lys Ala Lys Trp His Arg Arg Glu
405 410 415
Val His Tyr Thr Pro Gln Phe Glu Pro Thr Ser Lys Val Cys Asn Gln
420 425 430
Cys Gly Trp Lys Asn Glu Lys Leu Lys Leu Ser Asp Arg Gln Trp Arg
435 440 445
Cys Gly Gly Cys Glu Thr Ile His Asp Arg Asp Leu Asn Ala Ala Leu
450 455 460
Asn Ile Cys Arg Lys Ser Val Pro Lys Ser Ser Asp Asp Pro Ser Val
465 470 475 480
Ala Gly Lys Asp Glu Lys Asp Val Lys Ser Gly Ser Ala Lys Ser Arg
485 490 495
Arg Gly Arg Phe Val Ser Arg Lys Ser Glu Gly Asp Ser Ser Ser Pro
500 505 510
Phe Asp Pro Gly Ala Val Asp Thr Glu Gln Ser Val Val Gly Ala Pro
515 520 525
Asn Gly Gln Lys Ala Leu Glu Glu Ala Thr Gly His Arg Arg Asn Asn
530 535 540
Ala Asp Phe Ser Arg Ser Ser Gly Phe Lys Ser Arg Leu Asp Gly Tyr
545 550 555 560
<210> 18
<211> 532
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 18
Met Ser Tyr Leu Cys Ala Arg Gly Arg Phe Asn Leu Ser Gly Phe Ser
1 5 10 15
Thr Gly Ser Met Gly Val Leu Pro Val Arg Ala Ser Leu Phe Ala Leu
20 25 30
Leu Phe Thr Thr Leu His Gly Ala Arg Ile Ser Ala His Trp Tyr Gly
35 40 45
Met Ser Lys Glu Asn Asp His Thr Val Thr Cys Ile Lys Ile Cys Leu
50 55 60
Glu Pro Asn Lys Ala Gln Arg Ala Gln Phe Ala Ser Phe Ala Gly Ser
65 70 75 80
Ala Arg Trp Ala Tyr Asn Phe Ala Leu Ala Ile Lys Ile Gly Tyr Gln
85 90 95
Lys Arg Trp Phe Glu Ala Arg Lys Gln Phe Ile Glu Ser Gly Leu Asp
100 105 110
Glu Lys Ala Ala Gly Lys Lys Ala Ser Glu Gln Val Gly Arg Met Pro
115 120 125
Asn Tyr Met Ser Ile Ala Thr Asn Glu Trp Thr Gln Leu Arg Asp Glu
130 135 140
Val Cys Pro Trp Tyr Pro Glu Val Pro Arg Arg Val Phe Val Gly Gly
145 150 155 160
Phe Gln Arg Ala Asp Ala Ala Phe Lys Asn Trp Phe Asp Ser Lys Ser
165 170 175
Gly Arg Arg Ser Gly Ala Ala Met Gly Trp Pro Lys Phe Lys Ser Lys
180 185 190
Ser Lys Ser Arg Glu Ser Phe Val Ile Ala Asn Asp Val Gln Pro Ala
195 200 205
Phe Val Ala Asn Leu Asn Arg Tyr Ile Lys Thr Gly Glu Leu Ala Asp
210 215 220
Met Asp Tyr Arg His Ile Lys Val Pro Lys Cys Gly Glu Val Arg Leu
225 230 235 240
Thr Pro Gly Ser Ala Gly Gln Leu Arg Gln Leu Gly Arg Thr Met Leu
245 250 255
Ala Glu Ala Lys Thr Gly Glu Leu Ile Thr Arg Ile Thr Ser Gly Thr
260 265 270
Ile Ser Arg Leu Gly Asp Arg Trp Tyr Val Ser Leu Val Ile Ser Gly
275 280 285
Pro Phe Val Pro Asp Ala Ile Ser Thr Lys Arg Gln Arg Arg Asn Gly
290 295 300
Val Val Gly Val Asp Leu Gly Ser Gly Arg Phe Tyr Ala Thr Thr Ser
305 310 315 320
Glu Gly Leu Ser Ile Ile Asn Pro Lys Phe Val Ser Lys Tyr Glu Gln
325 330 335
Glu Leu Ala Arg Ala Asn Arg Ala Leu Ala Lys Thr Ala Lys Gly Ser
340 345 350
Ala Ala Arg Lys Lys Ala Leu Ala Arg Leu Arg Arg Val His Ala Arg
355 360 365
Ser Ala Leu Ala Arg Asp Gly Phe Ser His Gln Val Ser Ala Trp Leu
370 375 380
Thr Ser Gln Phe Ala Gly Val Ala Val Glu Lys Phe Asp Leu Ala Ser
385 390 395 400
Met Leu Ala Ser Ala Lys Gly Thr Val Glu Lys Pro Gly Lys Asn Val
405 410 415
Asp Val Lys Ala Arg Phe Asn Ala His Leu Ala Asp Val Gly Ile Ala
420 425 430
Ser Thr Ile Asp Lys Leu Leu Tyr Lys Gly Lys Arg Asp Gly Cys Arg
435 440 445
Val Gln Val Val Asn Thr Leu Asp Asn Ser Ser Thr Thr Cys Ala Lys
450 455 460
Cys Gly His Thr Cys Val Cys Gly Pro Glu Gln Lys Thr Phe Thr Cys
465 470 475 480
Pro Asp Cys Gly Tyr Asn Ala Pro Arg Gln Leu Asn Ser Ala Gln Tyr
485 490 495
Ile Arg Gln Leu Ala Thr Val Gly Phe Asp Glu Leu Gly Leu Asp Met
500 505 510
Thr Ala Ser Leu Thr Pro Asp Thr Gly Lys Arg Pro Ile Ala Phe Met
515 520 525
Thr Ser Ala His
530
<210> 19
<211> 579
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 19
Met Thr Gly Ala Lys Gln Lys Thr Pro Ile Arg Val Val Arg Phe Ser
1 5 10 15
Ile Asp His Ser Ala Leu Thr Pro Ala Gln Val Val Ala Phe Ala Arg
20 25 30
His Ala Gly Ala Ala Arg Gln Thr Trp Asn Trp Ala Leu Gly Arg Trp
35 40 45
Met Asp Trp Arg Asn Asn Thr Lys Phe Tyr Val Asp Tyr Lys Val Phe
50 55 60
Lys Ala Ala Gly Met Gly Pro Gly Leu Ser Thr Asp Asp Leu Ile Gln
65 70 75 80
Val Ile Glu Arg Ala Val Ser Ile Arg Gln Asp Asp Lys Trp Met Asp
85 90 95
Ala Ala Trp Asp Glu Ala Arg Gln Ile His Gly Glu Trp Asp Gln Phe
100 105 110
Gln Lys Ala Ser Thr Leu Gln Ser Leu Tyr Leu Ala Gly Ala Gln Glu
115 120 125
Pro Phe Asp Pro Ser Arg Asp Asp Gly Ile Asn Pro Tyr His Trp Trp
130 135 140
Val Thr Glu Gly Asp Lys Ser Gly Leu Pro Lys Ala Glu Arg His Asn
145 150 155 160
Val Asn Ser Gly Ala Thr Tyr Thr Ala Pro Leu Arg Ala Phe Glu Glu
165 170 175
Ala Val Gly Arg Phe Tyr Lys Leu Pro Gly Lys Lys Gly Thr Pro Lys
180 185 190
Phe Lys Ser Lys His Asp Asp Glu Gln Gly Phe Cys Ile Gln Arg Leu
195 200 205
Thr Glu Thr Gly Leu Ser Pro Trp Arg Ala Ile Glu Gly Gly His Arg
210 215 220
Ile Lys Val Pro Ser Ile Gly Ser Ile Arg Val Val Gln Ser Thr Lys
225 230 235 240
Arg Leu Arg Gln Leu Ile Lys Arg Gly Gly Lys Thr Thr Ser Ala Arg
245 250 255
Phe Thr Arg Arg Gly Gly Lys Trp Phe Val Ser Val Ser Val Ala Phe
260 265 270
Asp Leu Ser Ala Pro Arg Val Gln Arg Pro Ala Arg Leu Ser Arg Arg
275 280 285
Gln Arg Ala Gly Gly Ser Thr Gly Val Asp Leu Gly Val Asn Arg Leu
290 295 300
Ala Thr Leu Ser Ser Gly Asp Gln Phe Pro Asn Arg Arg Leu Leu Arg
305 310 315 320
Lys Ser Met Ala Glu Ile Lys Arg Leu Gln Arg Lys Phe Asp Arg Gln
325 330 335
His Arg Ala Gly Ser Pro Glu Cys Phe Asn Glu Asp Gly Thr His Lys
340 345 350
Lys Arg Cys Arg Trp Gly Arg Glu Asp Gly Pro Ala Met Ser Arg Ser
355 360 365
Ala Gln Thr Thr Lys Arg Gln Leu Arg Arg Ile His Asp Leu Thr Ala
370 375 380
Arg Arg Arg Ala Gly Val Leu His Glu Ile Thr Lys Asp Leu Ala Thr
385 390 395 400
Arg Phe Glu Leu Ile Gly Val Glu Asp Leu Asn Val Ala Gly Met Thr
405 410 415
Ala Lys Ser Lys Pro Lys Pro Asp Pro Asp Arg Pro Gly His Phe Leu
420 425 430
Pro Asn Arg Arg Ala Ala Lys Ala Gly Leu Asn Arg Ala Ile Leu Asp
435 440 445
Val Gly Phe Tyr Glu Phe Lys Arg Gln Leu Gly Tyr Lys Thr Glu Trp
450 455 460
Tyr Gly Ser Thr Met Gln Met Val His Arg Tyr Ala Ala Thr Ser Lys
465 470 475 480
Thr Cys Ser Gly Cys Gly Trp Val Lys Pro Lys Leu Thr Leu Ala Glu
485 490 495
Arg Thr Phe Asn Cys Thr Gln Cys Gly Leu Ala Met Asp Arg Asp His
500 505 510
Asn Ala Ala Val Asn Ile Arg Ala Leu Ala Leu Glu Gly Ala Ala Pro
515 520 525
Met Glu Arg Glu Gln Pro Ala Pro Val Gly Ala Ala Glu Lys Arg His
530 535 540
Arg Asp Pro Val Ser His Arg Arg Arg Pro Lys Ser Leu Ala Pro Cys
545 550 555 560
Glu Ser Thr Arg Pro Val Arg Asp Leu Ser Pro Pro Ala Thr Gln Glu
565 570 575
Glu Thr Ala
<210> 20
<211> 540
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 20
Met Ala Thr Thr Asp Lys Lys Asp Glu Glu Asn Leu Arg Ala Tyr Lys
1 5 10 15
Phe Arg Leu Asp Pro Asn Gln Ala Gln Thr Thr Ala Leu Tyr Gln Ala
20 25 30
Val Gly Ala Ala Arg Tyr Thr Tyr Asn Met Leu Thr Ala Tyr Asn Leu
35 40 45
Glu Val Asn Arg Leu Arg Asp Asp Tyr Trp Lys Arg Arg His Asp Glu
50 55 60
Asp Ile Ser Asp Ala Asp Ile Lys Lys Glu Leu Asn Ala Leu Ala Lys
65 70 75 80
Glu Asp Lys Arg Tyr Lys Gln Leu Asn Tyr Gly Ala Phe Gly Thr Gln
85 90 95
Tyr Leu Thr Pro Glu Lys Lys Arg His Glu Gln Ala Glu His Arg Ile
100 105 110
Glu Asn Gly Glu Asp Pro Ser Val Val Trp Asn Gln Glu Thr Glu Arg
115 120 125
Ser Ala Asn Pro Trp Leu His Thr Ala Asn Gln Arg Val Leu Val Ser
130 135 140
Gly Leu Gln Asn Ala Ser Asp Ala Trp Asp Asn Phe Trp Ala Ser Arg
145 150 155 160
Thr Gly Lys Arg Ala Gly Arg Leu Val Gly Thr Pro Arg Phe Lys Lys
165 170 175
Lys Gly Val Ser Arg Asp Ser Phe Thr Val Pro Ala Pro Glu Lys Met
180 185 190
Gly Ala Tyr Gly Thr Ala Tyr Leu Arg Gly Glu Pro Ala Tyr Lys Gln
195 200 205
Gly Arg Arg Lys Ile Thr Asp Tyr Arg His Val Arg Leu Ser Tyr Leu
210 215 220
Gly Thr Ile Arg Thr Phe Asn Ser Thr Lys Pro Leu Val Lys Ala Val
225 230 235 240
Val Ala Gly Ala Lys Ile Arg Ser Tyr Thr Val Ser Arg Asn Ala Asp
245 250 255
Arg Trp Tyr Val Ser Phe Leu Val Lys Phe Ser Glu Pro Ile Arg Arg
260 265 270
Ser Ala Thr Lys Arg Ala Arg Ala Ala Gly Ser Val Gly Val Asp Leu
275 280 285
Gly Val Lys Tyr Leu Ala Ser Leu Ser Asp Ser Glu Ala Pro Gln Arg
290 295 300
Phe Pro Asn Leu Lys Phe Val Glu Gly Leu Pro Ser Leu Glu Asn Pro
305 310 315 320
Arg Trp Ser Glu Ala Ser Ser Arg Arg Leu His Lys Leu Gln Arg Ala
325 330 335
Leu Ala Arg Ser Gln Lys Gly Ser Asn Arg Arg Ser Arg Leu Val Lys
340 345 350
Gln Ile Ala Arg Leu His His Met Thr Ala Leu Arg Arg Glu Ser Asn
355 360 365
Leu His Gln Leu Thr Lys Lys Leu Ala Thr Glu Tyr Thr Leu Val Gly
370 375 380
Phe Glu Asp Leu Asn Val Ser Gly Met Thr Ala Ser Ala Lys Gly Thr
385 390 395 400
Val Glu Asn Pro Gly Lys Asn Val Ala Gln Lys Ser Gly Leu Asn Arg
405 410 415
Val Val Leu Asp Ala Ala Phe Gly Val Phe Arg Asn Gln Leu Glu Tyr
420 425 430
Lys Ala Val Trp Tyr Gly Ser Ala Phe Glu Lys Val Asp Arg Tyr Phe
435 440 445
Ala Ser Ser Gln Thr Cys Ser Glu Cys Gly Arg Lys Ala Lys Thr Lys
450 455 460
Leu Thr Leu Arg Asp Arg Val Phe Asp Cys Ala Tyr Cys Gly Asn Met
465 470 475 480
Met Asp Arg Asp Leu Asn Ala Ala Val Asn Ile Cys Arg Glu Ala Gln
485 490 495
Arg Leu Phe Asp Glu Lys Leu Ala Ser Glu Asp Arg Glu Ser Leu Asn
500 505 510
Gly Arg Gly Ser Arg Gly Ala Leu Arg Gly Ala Glu Thr Val Glu Ala
515 520 525
Ser Arg Pro Pro Ala Ser His Arg Arg Gly Ser Pro
530 535 540
<210> 21
<211> 549
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 21
Met Ala Ala Ala Glu Cys Ser Thr Gln Lys Ala His Arg Ala Tyr Lys
1 5 10 15
Phe Arg Leu Ala Pro Asn Gln Lys Gln Leu Thr Ala Leu Tyr Gln Ala
20 25 30
Ala Gly Ala Ala Arg Tyr Ala Tyr Asn Gln Leu Thr Ala Tyr Asn Leu
35 40 45
Asp Ala Leu Arg Arg Arg Gln Gln Tyr Trp Gly Thr Arg Gln Asp Glu
50 55 60
Gly Ala Ser Glu Ser Glu Ile Lys Ala Glu Leu Arg Ala Ile Ala Arg
65 70 75 80
Glu Asp Ala Ser Tyr Arg Leu Leu Gly Tyr Ile Pro Tyr Ala Thr Glu
85 90 95
Tyr Leu Thr Pro Glu Arg Arg Arg His Gln Asp Ala Ala Ala Gln Ile
100 105 110
Ala Ala Gly Ala Gln Ala His Leu Ile Trp Gly Ala Asp Glu His Phe
115 120 125
Glu His Pro Trp Leu His Thr Ala Ser Arg Arg Val Leu Val Ser Gly
130 135 140
Leu Gln Ser Ala Asp Lys Ala Trp Lys Asn Phe Trp Asp Ser Arg Thr
145 150 155 160
Gly Ala Arg Ala Gly Arg Leu Met Gly Thr Pro Arg Phe Lys Lys Lys
165 170 175
Gly Phe Ser Arg Asp Ser Phe Thr Ile Pro Ala Pro Glu Ala Met Gly
180 185 190
Ala Tyr Gly Ala Ala Tyr Leu Arg Gly Glu Pro Ala Tyr Val Arg Gly
195 200 205
Arg Arg Thr Ile Thr Asp Tyr Arg His Val Arg Leu Ser His Leu Gly
210 215 220
Val Leu Arg Thr Tyr Asp Ser Thr Lys Pro Leu Val Lys Ala Val Ala
225 230 235 240
Ala Gly Ala His Ile Lys Ser Tyr Thr Val Ser Arg His Ala Glu Arg
245 250 255
Trp Tyr Val Ser Phe Leu Val Glu Leu Ser Gln Ser Pro Arg Arg Gln
260 265 270
Ala Ser Lys Arg Ala Arg Ala Ala Gly Thr Val Gly Val Asp Leu Gly
275 280 285
Val Arg Tyr Leu Ala Ser Leu Ser Asp Pro Gln Ala Pro Gln Arg Leu
290 295 300
Thr Gln Leu Asp Phe Leu Ala Asp Thr Pro Ser Ile Lys Asn Pro Arg
305 310 315 320
Trp Ala Asp Arg Ala Ala Arg Arg Leu His Arg Leu Gln Arg Ala Leu
325 330 335
Ala Arg Ser Gln Lys Gly Ser Val Arg Arg Ala Arg Ile Arg Gln Gln
340 345 350
Ile Ala Arg Leu His His Lys Thr Ala Leu Arg Arg Glu Ser Asn Leu
355 360 365
His Gln Leu Thr Lys Arg Leu Ala Thr Gly Tyr Thr Leu Ile Gly Val
370 375 380
Glu Asp Leu Asn Val Ala Gly Met Thr Ala Ser Ala Arg Gly Thr Val
385 390 395 400
Glu His Pro Gly Arg Arg Val Ala Gln Lys Ser Gly Leu Asn Arg Val
405 410 415
Val Leu Asp Ala Gly Phe Gly Val Phe Arg Arg Gln Leu Glu Tyr Lys
420 425 430
Ser Thr Trp Tyr Gly Ser Ala Val Gln Glu Ile Asp Arg Tyr Tyr Pro
435 440 445
Ser Ser Gln Ile Cys Ser Gln Cys Gln Arg Lys Ala Lys Thr Lys Leu
450 455 460
Ser Leu Arg Glu Arg Val Phe Glu Cys Ala Leu Cys Gly Tyr Lys Ala
465 470 475 480
Asp Arg Asp Phe Asn Ala Ala Val Asn Ile Ala Val Gln Ala Gln Arg
485 490 495
Val Phe Glu Glu Lys Leu Ala Ser Glu Ser Gly Glu Ser Leu Asn Gly
500 505 510
Arg Gly Gly Gly Arg Thr His Lys Gly Ala Val Val Ile Glu Ala Ser
515 520 525
Arg Pro Pro Ala Gly Tyr Gly Ser Gly Ser Pro Gln Val Ser Asn His
530 535 540
Leu Pro Ile Arg Thr
545
<210> 22
<211> 541
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 22
Met Thr Ala Thr Ala Ala Ala Glu Thr Val Met Asp Leu Arg Ala Tyr
1 5 10 15
Lys Phe Arg Leu Asp Pro Asn Ile Ala Gln Thr Gln Ala Leu Ser Gln
20 25 30
Ala Ala Gly Ala Ala Arg Val Ala Tyr Asn Met Val Ile Ala His Asn
35 40 45
Arg Ala Ala His Asp Glu Gly Arg Arg Arg Val Glu Glu Leu Thr Ala
50 55 60
Ser Gly Val Asp Pro Glu Glu Ala Ala Ala Arg Val Arg Ala Gln Arg
65 70 75 80
Thr Thr Asp Pro Ala Leu Ala Met Val Phe Thr Lys Met Gly Phe Asn
85 90 95
Ala Ile Leu Thr Ala Glu Arg Arg Arg His Gln Glu Ala Ala Glu Ala
100 105 110
Ile Ala Ala Gly Ala Asp Pro Ala Gln Val Trp Ala Gly Gln Arg Tyr
115 120 125
Glu Arg Pro Trp Leu His Asp Ala Asn Arg Arg Val Met Val Ser Gly
130 135 140
Val Glu His Ala Thr Ala Ala Phe Thr Asn Trp Val Asn Ser Tyr Thr
145 150 155 160
Gly Gln Arg Ala Gly Arg Arg Val Gly Tyr Pro Arg Phe Lys Lys Lys
165 170 175
Thr Val Ser Arg Asp Ser Phe Thr Ile Pro Ala Pro Glu Leu Met Gly
180 185 190
Gly Lys Gly Ala Pro Tyr Lys Arg Gly Glu Ala Arg Lys Gly Leu Ile
195 200 205
Thr Asp Tyr Arg His Leu Arg Leu Gly Phe Leu Gly Thr Ile Arg Thr
210 215 220
His Gln His Thr Arg Arg Leu Val Arg Ala Cys Gln Arg Gly Ala Val
225 230 235 240
Met Arg Ser Phe Thr Val Ser Arg Ala Ala Asp Arg Trp Tyr Val Ser
245 250 255
Ile Leu Val Ala Thr Pro Arg Glu Thr Ala Pro Glu Pro Thr Arg Ala
260 265 270
Gln Arg Ala Arg Gly Gly Val Gly Val Asp Leu Gly Val Asn Asn Leu
275 280 285
Ala Ala Leu Ser Thr Gly Glu Leu Val Asp Asn Pro Arg Tyr Gly Arg
290 295 300
Arg Gly Ala Ala Lys Leu Arg Arg Ala Gln Arg Ala Tyr Ala Arg Thr
305 310 315 320
Lys Lys Gly Ser Gln Gly Arg Ala Arg Ala Ala Arg Arg Val Ala Arg
325 330 335
Leu His His Leu Val Ala Leu Gln Arg Ser Thr Val Val Asn Thr Leu
340 345 350
Thr Lys Arg Leu Ala Thr Gly Tyr Ala Val Val Ala Ile Glu Asp Leu
355 360 365
Asn Val Ser Gly Met Thr Arg Ser Ala Lys Gly Thr Val Glu Asn Pro
370 375 380
Gly Lys Asn Val Ala Ala Lys Ser Gly Leu Asn Arg Ser Val Leu Asp
385 390 395 400
Ala Ala Phe Gly Glu Leu Arg Arg Gln Leu Glu Tyr Lys Thr Ala Trp
405 410 415
Tyr Gly Ser Ser Val Ala Thr Ile Gly Arg Tyr Glu Pro Ser Ser Lys
420 425 430
Ala Cys Ser Ser Cys Gly Ala Val Lys Pro Lys Leu Ser Leu Gly Glu
435 440 445
Arg Val Tyr Asp Cys Asp Gln Cys Gly Met Arg Leu Asp Arg Asp Val
450 455 460
Asn Ala Ala Arg Asn Ile Leu Ala Trp Ala Thr Gly Gly Arg Asp Gln
465 470 475 480
Pro Pro Ala Pro Gly Thr Gly Ala Asp Gly Arg Gly Glu Pro Ala Pro
485 490 495
Pro Ser Pro Pro Gly Glu Gly Pro Pro Ala Pro Val Gln Arg Ser Ala
500 505 510
Lys Thr Gly Tyr Val Pro His Pro Ala Thr Ala Ala Glu Gln Ser Ala
515 520 525
Ala His Pro His Gln Ser Arg Thr Pro Ile Pro Pro Ser
530 535 540
<210> 23
<211> 558
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 23
Met Ala Glu Lys Thr Gly Thr Asp Ala Gly Thr Met Asn Arg Ala Tyr
1 5 10 15
Lys Phe Arg Leu Asp Pro Asn Gln Ala Gln Lys Ala Glu Leu Met Arg
20 25 30
Cys Val Gly Ala Ala Arg Tyr Thr Tyr Asn Leu Leu Asn Ala Tyr Asn
35 40 45
Leu Gln Ile Leu Arg Asn Glu Gln Glu Tyr Arg Asn Thr Arg Asn Ala
50 55 60
Glu Gly Ala Asp Tyr Glu Thr Ile Asn Gly Glu Ile Arg Lys Leu Arg
65 70 75 80
Lys Lys Asp Pro Ala Tyr Lys Phe Leu Gly His Ala Glu Tyr Glu Lys
85 90 95
Arg Tyr Leu Thr Pro Glu Lys Gln Arg His Glu Ala Ile Ala Gln Ala
100 105 110
Ile Ala Asp Gly Ala Asp Pro Ala Ile Val Trp Pro Glu Thr Glu Arg
115 120 125
Phe Thr Glu Pro Trp Leu His Thr Ile Ala Arg Arg Val Leu Val Ser
130 135 140
Gly Ile Lys Asn Ala Asp Lys Ala Trp Asp Asn Tyr Asn Lys Ser Arg
145 150 155 160
Met Lys Gln Arg Ala Gly Ala Arg Ile Gly Ile Pro Arg Phe Lys Arg
165 170 175
Lys Gly Val Ser Arg Asp Ser Phe Thr Val Pro His Glu Thr Thr Gly
180 185 190
Ala Tyr Gly Ala Tyr Tyr His Lys Lys Asp Pro Glu Tyr Ala Arg Arg
195 200 205
Lys Ala Gln Leu Lys Arg Arg Gly Ile Ser Val Lys Pro Thr Ile Thr
210 215 220
Asp Tyr Arg His Val Arg Leu Ala Ser Leu Gly Val Ile Arg Thr His
225 230 235 240
Asn Thr Thr Arg Pro Leu Val Lys Ala Val Arg Ala Gly Ala Glu Ile
245 250 255
Lys Ser Phe Thr Val Ser Arg Ala Ala Asp Arg Trp Tyr Val Ser Ile
260 265 270
Leu Val Glu Leu Thr Arg Pro Ser Thr Ala Pro Thr Arg Ala Gln Arg
275 280 285
Ala Ala Gly Ala Val Gly Val Asp Leu Gly Val Arg Tyr Leu Ala Ala
290 295 300
Leu Ser Asp Glu Gln Ala Pro Gln Arg Phe Ala Arg Tyr Pro Asp Leu
305 310 315 320
Glu Phe Thr Gly Asp Gly Asp Pro Thr Leu Ala Asn Pro Arg Trp Ala
325 330 335
Arg Thr Ala Glu Lys Arg Leu Val Arg Leu Gln Arg Ala Leu Ala Arg
340 345 350
Ala Gln Lys Gly Ser Asn Arg Arg Ala Arg Ile Val Gln Gln Ile Ala
355 360 365
Arg His His His Leu Val Ala Leu Arg Arg Glu Ser Gly Leu His Gln
370 375 380
Val Ser Lys Arg Leu Thr Thr Gly Tyr Thr Leu Ile Gly Leu Glu Asp
385 390 395 400
Leu Ala Val Ala Gly Met Thr Ala Ser Ala Ala Gly Thr Ile Glu Ala
405 410 415
Pro Gly Lys Asn Val Arg Gln Lys Ala Gly Leu Asn Arg Ser Ile Leu
420 425 430
Asp Ala Ala Leu Ser Ala Leu Arg Arg Gln Leu Glu Tyr Lys Ser Ser
435 440 445
Trp Tyr Gly Ser Gln Val Gln Ile Ile Asp Arg Phe Phe Ala Ser Ser
450 455 460
Gln Thr Cys Ser Ala Cys Gly Ala Arg Ala Lys Thr Lys Leu Gly Leu
465 470 475 480
His Val Arg Val Phe Glu Cys Ala Ala Cys Gly Ala Arg Ile Asp Arg
485 490 495
Asp Val Asn Ala Ala Arg Asn Ile Arg Ala Glu Ala Val Arg Met Tyr
500 505 510
Glu Ala Gln Leu Ala Pro Gly Thr Gly Glu Ser Leu Asn Gly Arg Gly
515 520 525
Ala Thr Asp Ser Asp Ala Ala Gly Ser Val Val Leu Gly Asp Ala Ala
530 535 540
Leu Asp Ala Ser Arg Pro Ala Ala Thr Gly Gly Gly Ser Pro
545 550 555
<210> 24
<211> 559
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 24
Met Ala Lys Lys Ile Ser Thr Asp Ala Asp Thr Thr Leu Arg Ala Tyr
1 5 10 15
Lys Phe Arg Leu Asp Pro Thr Gln Thr Gln Lys Ile Ala Leu Ala Gln
20 25 30
Cys Ala Gly Ala Ala Arg Tyr Ala Tyr Asn Leu Leu Thr Ala His Asn
35 40 45
Leu Glu Val Ser Arg Val Arg Ala Asp Tyr Trp His Thr Arg Ile Asp
50 55 60
Ala Gly Ala Glu Glu Thr Thr Val Lys Ala Glu Leu Lys Ala Leu Ala
65 70 75 80
Lys Ser Asp Ala Ala Tyr Lys Ser Ile Gly Tyr Ala Ala Tyr Gly Thr
85 90 95
Gln Tyr Leu Thr Pro Glu Ile Asn Arg His Arg Ala Ala Ser Asn Ala
100 105 110
Ile Thr Asp Gly Ala Asp Pro Ala Ala Val Trp Pro Glu Thr Glu Arg
115 120 125
Ser Ser Glu Pro Trp Met His Thr Ile Pro Arg Arg Val Leu Val Ser
130 135 140
Gly Leu Gln Ser Ala Asp Lys Ala Trp Lys Asn Phe Phe Asp Ser Arg
145 150 155 160
Thr Gly Ala Arg Ala Gly Arg Leu Val Gly Thr Pro Arg Phe Lys Arg
165 170 175
Lys Gly Ala Ser Arg Asp Ser Phe Thr Ile Pro Ala Pro Glu Thr Ile
180 185 190
Gly Ala Tyr Gly Thr Ala Tyr Leu Arg Gly Glu Pro Glu Tyr Ala Arg
195 200 205
Arg Lys Ala Gln Leu Lys His Arg Gly Ile Lys Thr Ala Pro Thr Ile
210 215 220
Glu Asp Tyr Arg His Val Arg Leu Ser His Leu Gly Val Ile Arg Thr
225 230 235 240
His Asn Thr Thr Lys Pro Leu Val Lys Ala Val Arg Ala Gly Ala Gln
245 250 255
Ile Arg Ser Tyr Thr Val Ser Arg Ala Ala Asp Arg Trp Tyr Val Ser
260 265 270
Ile Leu Val Glu Leu Thr Arg Pro Ser Ala Thr Pro Thr Arg Ala Gln
275 280 285
Arg Ser Ala Gly Ala Val Gly Val Asp Leu Gly Val Arg Tyr Leu Ala
290 295 300
Ala Leu Ser Asp Glu Gln Ala Pro Gln Arg Phe Ala Arg Phe Pro Ser
305 310 315 320
Leu Glu Phe Thr Gly Asp Gly Ala Pro Thr Leu Ala Asn Pro Arg Trp
325 330 335
Ala Arg Ala Ala Glu Lys Arg Leu Val Arg Leu Gln Arg Ala Leu Ser
340 345 350
Arg Ala Gln Lys Gly Ser Asn Arg Arg Ala Arg Ile Val Gln Gln Ile
355 360 365
Ala Arg His His His Leu Val Ala Leu Arg Arg Glu Ser Gly Leu His
370 375 380
Gln Val Ser Lys Arg Leu Ser Thr Gly Tyr Thr Leu Ile Gly Leu Glu
385 390 395 400
Asp Leu Ala Val Ala Gly Met Thr Ala Ser Ala Ala Gly Thr Ile Glu
405 410 415
Ala Pro Gly Lys Asn Val Arg Gln Lys Ala Gly Leu Asn Arg Ser Ile
420 425 430
Leu Asp Ala Ala Phe Ser Thr Leu Arg Arg Gln Leu Glu Tyr Lys Ala
435 440 445
Ser Trp Tyr Gly Ser Gln Val Gln Ile Ile Asp Arg Phe Phe Ala Ser
450 455 460
Ser Gln Thr Cys Ser Ala Cys Gly Ala Arg Ala Lys Thr Lys Leu Asp
465 470 475 480
Leu Arg Val Arg Val Phe Glu Cys Ala Ala Cys Gly Val Arg Ile Asp
485 490 495
Arg Asp Val Asn Ala Ala Arg Asn Ile Arg Ala Glu Ala Val Arg Met
500 505 510
Tyr Glu Ala Gln Leu Ala Pro Gly Thr Gly Glu Ser Leu Asn Gly Arg
515 520 525
Gly Val Ala Gly Ser Asp Ala Ala Gly Leu Val Val Leu Gly Asp Val
530 535 540
Ala Leu Asp Ala Ser Arg Pro Ala Ala Thr Gly Gly Gly Ser Pro
545 550 555
<210> 25
<211> 559
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 25
Met Ala Lys Lys Thr Ser Thr Asp Ala Glu Thr Met Asn Arg Ala Tyr
1 5 10 15
Lys Phe Arg Leu Asp Pro Thr Gln Ala Gln Lys Ala Glu Leu Met Arg
20 25 30
Cys Ala Gly Ala Ala Arg Cys Thr Tyr Asn Leu Leu Asn Glu Tyr Asn
35 40 45
Leu Gln Ile Leu Arg Asn Glu Gln Glu Tyr Arg Arg Thr Arg Ala Ala
50 55 60
Glu Gly Ile Asp His Lys Thr Ile Thr Ser Glu Leu Lys Lys Leu Gly
65 70 75 80
Lys Lys Asp Pro Glu Tyr Lys Phe Leu Gly Gln Ala Glu Tyr Glu Lys
85 90 95
Arg Tyr Leu Thr Pro Glu Lys Gln Arg His Glu Ala Ile Ala Gln Ala
100 105 110
Ile Ala Asp Gly Ala Asp Pro Ala Ile Val Trp Pro Glu Thr Glu Arg
115 120 125
Phe Thr Glu Pro Trp Leu His Thr Ile Ala Arg Arg Val Leu Val Ser
130 135 140
Gly Ile Lys Asn Ala Asp Lys Ala Trp Asp Asn Tyr Asn Lys Ser Arg
145 150 155 160
Met Lys Gln Arg Ala Gly Ala Arg Met Gly Val Pro Arg Arg Lys Arg
165 170 175
Lys Gly Val Ser Arg Asp Ser Phe Thr Ile Pro Ala Pro Glu Ala Ile
180 185 190
Gly Ala Tyr Gly Thr Tyr Tyr His Lys Lys Asp Pro Glu Tyr Ala Arg
195 200 205
Arg Lys Ala Gln Leu Lys Arg Arg Gly Ile Asn Ala Ala Pro Thr Ile
210 215 220
Glu Asp Tyr Arg His Val Arg Leu Ser His Leu Gly Val Ile Arg Thr
225 230 235 240
His Asn Thr Thr Lys Pro Leu Val Lys Ala Val Arg Ala Gly Ala Gln
245 250 255
Ile Arg Ser Tyr Thr Val Ser Arg Thr Ala Asp Arg Trp Tyr Val Ser
260 265 270
Ile Leu Val Glu Leu Thr Arg Pro Ser Ala Thr Pro Thr Arg Ala Gln
275 280 285
Arg Ala Ala Gly Ala Val Gly Val Asp Leu Gly Val Arg Tyr Leu Ala
290 295 300
Ala Leu Ser Asp Glu Trp Ala Pro Gln Arg Phe Ala Arg Tyr Pro Ser
305 310 315 320
Leu Glu Phe Thr Gly Asp Gly Ala Pro Ile Leu Ala Asn Pro Cys Trp
325 330 335
Ala Arg Ala Ala Glu Arg Arg Leu Val Arg Leu Gln Arg Ala Leu Ala
340 345 350
Arg Ala Gln Lys Gly Ser Arg Arg Arg Ala Arg Leu Val Gln Gln Ile
355 360 365
Ala Arg His His His Leu Val Ala Leu Arg Arg Glu Ser Gly Leu His
370 375 380
Gln Val Ser Lys Arg Leu Ser Thr Gly Tyr Thr Leu Ile Gly Leu Glu
385 390 395 400
Asp Leu Ala Val Thr Gly Met Thr Ala Ser Ala Ala Gly Thr Val Glu
405 410 415
Ala Pro Gly Lys Asn Val Arg Gln Lys Ala Gly Leu Asn Arg Ser Ile
420 425 430
Leu Asp Ala Ala Phe Ser Thr Leu Arg Arg Gln Leu Glu Tyr Lys Ser
435 440 445
Gly Trp Tyr Gly Ser Gln Val Gln Ile Ile Asp Arg Phe Phe Ala Ser
450 455 460
Ser Gln Thr Cys Ser Ala Cys Gly Ala Arg Ala Lys Thr Lys Leu Asp
465 470 475 480
Leu Arg Val Arg Val Phe Glu Cys Ala Ala Cys Gly Val Arg Ile Asp
485 490 495
Arg Asp Val Asn Ala Ala Arg Asn Ile Arg Ala Glu Ala Val Arg Met
500 505 510
Tyr Glu Ala Gln Leu Ala Pro Gly Thr Gly Glu Ser Leu Asn Gly Arg
515 520 525
Gly Ala Ala Gly Ser Asp Ala Ala Gly Ser Val Val Leu Gly Asp Val
530 535 540
Ala Leu Asp Ala Ser Arg Pro Ala Ala Thr Gly Gly Gly Ser Pro
545 550 555
<210> 26
<211> 485
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 26
Met Thr Thr Glu Ile Ala His Lys Ala Phe Lys Tyr Arg Leu Lys Pro
1 5 10 15
Thr Gln Thr Gln Glu Asn Ala Phe Arg Gln Ala Val Gly Ala Ala Arg
20 25 30
Val Ala Tyr Asn Met Leu Thr Ala Leu Asn Arg Asp Val Leu Arg Arg
35 40 45
Gly Trp Glu Ile Arg Asn Gln Leu Ile Glu Gln Gly Leu Ser Glu Ala
50 55 60
Glu Ala Ser Ala Gln Met Lys Lys Leu Arg Lys Glu Asp Leu Ser Leu
65 70 75 80
Lys Ile Met Ser Ser Phe Ala Trp Gln Lys Asp Val Leu Thr Pro Glu
85 90 95
Ile Ala Arg His Lys Gln Ala Ala Ala Arg Ile Ala Ala Gly Asp Pro
100 105 110
Ile Glu Ser Val Trp Ser Gly Glu Arg Phe Ala Glu Pro Trp Phe His
115 120 125
Ala Val Pro Arg Arg Ala Phe Val Ser Gly Ala Asp Gln Ala Ala Thr
130 135 140
Ala Ile Ser Asn Trp Met Lys Ser Val Ala Gly Gln Arg Ala Gly Asn
145 150 155 160
Lys Met Gly Leu Pro Arg Phe Lys Lys Ala Gly Arg Ser His Asp Ser
165 170 175
Ile Thr Ile Pro Val Val Asp Gly Ser Ser Pro Ala Gly Gly Tyr Gly
180 185 190
Ala Ser Tyr Lys Arg Gly Glu Pro Arg Lys Gly Phe Ile Gln Asp His
195 200 205
His Arg Leu Arg Leu Ala Met Phe Gly Thr Val Val Thr Tyr Asn Ser
210 215 220
Thr Ala Pro Leu Thr Gln Leu Val Thr Gln Gly Gly Thr Ile Lys Ser
225 230 235 240
Phe Thr Ile Ser Arg Asp Ala Gln Tyr Trp Tyr Val Ser Leu Leu Val
245 250 255
Glu Ala Pro Ile Asn Leu Leu His Arg Pro Gly Pro Thr Lys Ala Gln
260 265 270
Glu Lys Ala Gly Val Ile Gly Val Asp Leu Gly Val Lys Ala Lys Ala
275 280 285
Ala Leu Ser Asn Gly Glu Ile Ile Asn Asn Pro Arg His Leu Gln Leu
290 295 300
Ser Leu Lys Arg Leu Ala Lys Leu Gln Val Lys Leu Ser Arg Thr Gln
305 310 315 320
Lys Gly Ser Lys Asn Arg Glu His Leu Lys Lys Gln Ile Ser Arg Leu
325 330 335
Gln His Lys Ile Ala Leu Gln Arg Ala Ala Ser Asn His Gln Met Thr
340 345 350
Lys Glu Leu Ser Thr Thr Tyr Val Gly Val Gly Ile Glu Asp Leu Asn
355 360 365
Val Ala Gly Met Thr Lys Ser Ala Ser Gly Thr Leu Glu Asn Pro Gly
370 375 380
Lys Asn Val Ala Ala Lys Ser Gly Leu Asn Arg Asn Ile Leu Asp Val
385 390 395 400
Ser Phe Gly Gln Ile Arg Asn Gln Leu Glu Tyr Lys Thr Lys Met Tyr
405 410 415
Gly Ser Ala Leu Thr Val Ile Gly Arg Tyr Glu Pro Thr Ser Lys Arg
420 425 430
Cys Ser Gln Cys Gly Ala Met Lys Ser Asp Leu Ser Leu Lys Glu Arg
435 440 445
Thr Tyr Ile Cys Val Glu Cys Asn Tyr Gln Asp Asp Arg Asp Ile Asn
450 455 460
Ala Ala Lys Asn Ile Arg Asn Leu Ala Glu Lys Glu Leu Gly Met Val
465 470 475 480
Ser Ser Ile Ser Ser
485
<210> 27
<211> 562
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 27
Met Ala Lys Lys Thr Ser Thr Asp Asp Gly Ala Leu Tyr Arg Ala Tyr
1 5 10 15
Lys Phe Arg Leu Asp Pro Asn Gln Ala Gln Lys Ala Lys Leu Met Gln
20 25 30
Cys Val Gly Ala Ala Arg Tyr Thr Tyr Asn Leu Phe Thr Asp His Asn
35 40 45
Arg Lys Val Ser Arg Ala Cys Ala Glu Tyr Val Asp Thr Arg Ile Ser
50 55 60
Glu Gly Ala Thr Glu Val Ile Ala Lys Ala Glu Leu Asp Asp Ile Lys
65 70 75 80
Glu Glu Asn Pro Ala Tyr Lys Tyr Ile Gly His Phe Glu Tyr Gly Lys
85 90 95
Arg Tyr Leu Thr Pro Glu Arg Gln Arg His Glu Ala Ile Ala Gln Ala
100 105 110
Ile Ala Asp Gly Ala Asp Pro Ala Val Val Trp Pro Glu Thr Glu Arg
115 120 125
Ser Ser Glu Pro Trp Met His Thr Ile Asp Arg Arg Val Leu Val Ser
130 135 140
Gly Ile Lys Asn Ala His Ala Ala Trp Gly Asn Tyr Phe Glu Ser Leu
145 150 155 160
Lys Gly Leu Arg Ala Gly Pro Arg Met Gly Val Pro Arg Phe Lys Arg
165 170 175
Lys Gly Ile Ser His Asp Ser Phe Thr Val Asp Arg Asp Pro Arg Asn
180 185 190
Asp Glu Leu Gly Gly Tyr Gly Thr Pro Tyr Lys Lys Gly Thr Pro Glu
195 200 205
Tyr Ala Arg Arg Thr Ala Gln Leu Lys Arg Arg Gly Ile Lys Thr Ile
210 215 220
Pro Thr Ile Glu Asp Tyr Arg His Val Arg Leu Ala Ser Leu Gly Val
225 230 235 240
Ile Arg Thr His Asn Ser Thr Lys Pro Leu Val Lys Ala Val Arg Ala
245 250 255
Gly Ala Asp Val Lys Ser Phe Thr Val Ser Arg Ala Ala Asp Arg Trp
260 265 270
Tyr Val Ser Ile Leu Val Lys Leu Ser Arg Thr Pro Val Thr Pro Thr
275 280 285
Arg Ala Gln Arg Ala Ala Gly Ala Val Gly Val Asp Leu Gly Val Arg
290 295 300
Tyr Leu Ala Ala Leu Ser Asp Glu Gln Ala Pro Gln Arg Phe Ala Gln
305 310 315 320
Tyr Pro Ser Leu Glu Phe Thr Gly Asp Gly Ser Pro Thr Leu Ala Asn
325 330 335
Pro Arg Trp Ala Arg Ala Ala Glu Lys Arg Leu Val Arg Leu Gln Arg
340 345 350
Ala Leu Ala Arg Ala Gln Lys Gly Ser Asn Arg Arg Ala Arg Ile Val
355 360 365
Gln Lys Ile Ala Arg His His His Leu Val Ala Leu Arg Arg Glu Ser
370 375 380
Gly Leu His Gln Val Ser Lys Arg Leu Ala Thr Gly Tyr Thr Leu Ile
385 390 395 400
Gly Leu Glu Asp Leu Ala Val Ala Gly Met Thr Ala Ser Ala Ala Gly
405 410 415
Thr Ile Glu Ala Pro Gly Lys Asn Val Arg Gln Lys Ala Gly Leu Asn
420 425 430
Arg Ser Ile Leu Asp Ala Ala Phe Ser Thr Leu Arg Arg Gln Leu Glu
435 440 445
Tyr Lys Ala Ser Trp Tyr Gly Ser Gln Val Gln Ile Ile Asp Arg Phe
450 455 460
Phe Ala Ser Ser Gln Thr Cys Ser Ala Cys Gly Ala Arg Val Lys Thr
465 470 475 480
Lys Leu Gly Leu His Val Arg Val Phe Glu Cys Ala Ala Cys Gly Val
485 490 495
Arg Ile Asp Arg Asp Val Asn Ala Ala Arg Asn Ile Arg Ala Glu Ala
500 505 510
Val Arg Met Cys Glu Ala Gln Leu Ala Pro Gly Thr Gly Glu Ser Leu
515 520 525
Asn Gly Arg Gly Val Ala Asp Ser Asn Val Ala Gly Ser Val Val Leu
530 535 540
Gly Asp Ala Ala Leu Asp Ala Ser Arg Pro Ala Ala Thr Gly Gly Gly
545 550 555 560
Ser Pro
<210> 28
<211> 494
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 28
Met Ser Ser Gly Thr Thr Ala Arg Arg Met Arg Ala Tyr Arg Phe Thr
1 5 10 15
Leu Asp Pro Thr Arg Thr Gln Leu Asp Thr Leu Ala Gln His Ala Gly
20 25 30
Ala Ala Arg Trp Ala Tyr Asn His Ala Leu Ala Thr Lys Leu Asp Ala
35 40 45
Leu Arg Arg Arg Gln Leu Gln Ile Asp Glu Leu Val Thr Leu Gly Leu
50 55 60
Thr Glu Gln Gln Ala Arg Arg Asp Ala Thr Val Thr Val Pro Ser Lys
65 70 75 80
Pro Gln Val Gln Lys Arg Trp Asn Gln Leu Lys Gly Asp Thr Thr Arg
85 90 95
Gly Gly Asp Gly Ile Cys Pro Trp Trp Arg Ala Val Ser Thr Tyr Ala
100 105 110
Phe Gln Ser Ala Phe Leu Asp Ala Asp Arg Ala Trp Lys Asn Trp Met
115 120 125
Asp Ser Leu Thr Gly Lys Arg Ala Gly Arg Arg Val Gly Ala Pro Arg
130 135 140
Phe Lys Lys Lys Gly Arg Cys Arg Asp Ser Phe Arg Leu His His Ala
145 150 155 160
Val Asn Asp Pro Thr Ile Arg Leu Glu Gly Tyr Arg Arg Leu Arg Met
165 170 175
Pro Arg Leu Gly Ser Ile Arg Leu His Asp Ser Gly Lys Arg Leu Ala
180 185 190
Arg Ala Leu Ala Arg Gly Gly Arg Val Gln Ser Val Thr Val Ser Arg
195 200 205
Gly Gly His Arg Trp Tyr Ala Ser Val Leu Val Asp Glu Pro Asp His
210 215 220
Thr Pro Gly Arg Glu Thr Gln His Arg Pro Ser Arg Ala Gln His Thr
225 230 235 240
Ala Gly Gly Val Gly Val Asp Val Gly Val His His Leu Ala Ala Leu
245 250 255
Ser Thr Gly Glu Thr Leu Asp Asn Pro Arg His Leu His His Ala His
260 265 270
Thr Arg Leu Val Lys Ala Gln Arg Ala Leu Ala Arg Thr Gln Lys Gly
275 280 285
Ser His Arg Arg Arg Arg Ala Ala Glu Arg Val Gly Arg Leu His His
290 295 300
Gln Leu Ala Glu Arg Arg Ala Ser His Leu His Thr Ile Thr Lys Arg
305 310 315 320
Leu Ala Thr Gln His Ala Leu Val Ala Val Glu Asp Leu Asn Val Gln
325 330 335
Gly Met Thr Arg Thr Ala Arg Gly Thr Leu Thr Gln Pro Gly Arg Asn
340 345 350
Val Arg Ala Lys Ala Gly Leu Asn Arg Ala Ile Leu Asp Ala Ala Pro
355 360 365
Gly Glu Leu Arg Arg Gln Leu Glu Tyr Lys Ala Ser Trp Tyr Gly Ser
370 375 380
Thr Leu Ala Val Cys Asp Arg Trp Ala Pro Thr Ser Lys Thr Cys Ser
385 390 395 400
Thr Cys Gly Thr Val Lys Thr Lys Leu Pro Leu Ser Thr Arg Val Tyr
405 410 415
Arg Cys Asp Thr Cys Gly Met Val Cys Asp Arg Asp Ile Asn Ala Ala
420 425 430
Arg Asn Ile Leu Lys Asp Ala Asp Pro Val Ala Pro Gly Arg Gly Glu
435 440 445
Thr Leu Asn Ala Cys Gly Gly Pro Val Ser Pro Ser Ala Pro His Ala
450 455 460
Pro Thr Ala Arg Pro Ser Glu Ala Gly Arg Pro Gly His Pro Ala Arg
465 470 475 480
Ser Pro Arg Gly Asn Asp Pro Pro Ser Thr Pro Lys Thr Glu
485 490
<210> 29
<211> 606
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 29
Met Ala Gln Ala Glu Ala Pro Arg Arg Leu Arg Ala Tyr Lys Phe Ala
1 5 10 15
Leu Asp Pro Thr Glu Ala Gln Leu Arg Glu Phe Glu Gln His Ala Gly
20 25 30
Ser Ala Arg Trp Ala Tyr Asn His Ala Asn Ala Ile Leu Ser Arg Tyr
35 40 45
Ser Asp Thr Leu Arg Asn Arg Trp Asn Ala Trp Ile Ala Gln His His
50 55 60
Gly Leu Ser Arg Glu Gln Leu Tyr Ala Leu Pro Asp Arg Glu Arg Thr
65 70 75 80
Ala Ile Gln Ala Ala Ala Arg Ala Ala Val Lys Ala Glu Asn Ala Gln
85 90 95
Leu Ala Ala Glu Leu Arg Ile Ile Asp Asp His Arg Lys Arg Val Thr
100 105 110
His Lys Gly Lys Pro Ser Val Glu Pro Gly Glu Gln Pro Ala Glu Asp
115 120 125
Ala Pro Glu Arg Ala Tyr Gln Leu Trp Arg Glu Arg Val Glu Leu Ala
130 135 140
Arg Leu His Ala Glu Asp Pro Gln Ala Tyr Arg Ala Glu Arg Lys Arg
145 150 155 160
Ile Leu Asp Glu Ile Arg Pro Leu Val Asn Ala Thr Lys Arg Lys Leu
165 170 175
Ile Glu Gln Gly Ala Tyr Arg Pro Thr Ala Met Asp Ile Ser Thr Leu
180 185 190
Trp Arg Glu Ile Arg Asp Leu Pro Pro Asp Glu Gly Gly Ser Pro Trp
195 200 205
Trp Pro Glu Val Ser Ile Tyr Ala Phe Thr Ser Gly Phe Ala His Ala
210 215 220
Glu Thr Ala Trp Lys Asn Tyr Leu Glu Ser Leu Ala Gly Arg Arg Ala
225 230 235 240
Gly Arg Pro Val Gly Lys Pro Arg Phe Lys Lys Lys Arg Arg Ser Arg
245 250 255
Arg Ser Phe Thr Leu Tyr Gly Ser Val Lys Leu Val Thr Tyr Arg Arg
260 265 270
Ile Gln Val Pro Ser Ile Gly Ser Val Arg Leu His Gly Ser Ala Lys
275 280 285
Arg Leu His Arg Ala Leu Glu Arg Arg Gly Gly Ile Ile Lys Ser Ile
290 295 300
Thr Ile Ser Gln Gly Gly His Arg Trp Tyr Ala Ser Val Leu Val Asp
305 310 315 320
Glu Leu Asp Ile Thr Pro Gly Arg Glu Thr Gln Arg Gly Pro Ser Arg
325 330 335
Arg Gln Arg Asp Arg Gly Ala Val Gly Val Asp Leu Gly Val His His
340 345 350
Leu Val Ala Leu Ser Asp Pro Asn Glu Lys Thr Leu Asp Asn Pro Arg
355 360 365
His Leu Arg Lys Ala Arg Lys Arg Leu Leu Lys Ala Gln Arg Ala Met
370 375 380
Ser Arg Arg Arg Gly Pro Asp Lys Arg Thr Gly Gln Glu Pro Ser Arg
385 390 395 400
Arg Trp Val Lys Ala Arg Asn Arg Val Ala Arg Leu His His Glu Leu
405 410 415
Ala Val Arg Arg Ala Gly His Leu His Glu Ile Thr Lys Arg Leu Ala
420 425 430
Thr Ser Tyr Glu Leu Val Ala Ile Glu Asp Leu Asn Val Ala Gly Met
435 440 445
Thr Arg Ser Ala Arg Gly Thr Ile Asp Gln Pro Gly Arg Gly Val Arg
450 455 460
Ala Lys Ala Gly Leu Asn Arg Ser Ile Leu Asp Thr Ser Pro Ala Glu
465 470 475 480
Phe Arg Arg Gln Leu Gln Tyr Lys Ala Ser Trp Tyr Gly Ala Thr Val
485 490 495
Ala Val Ile Asp Arg Trp Ala Pro Thr Ser Arg Thr Cys Ser Ser Cys
500 505 510
Gly Ala Val Lys Ala Lys Leu Ser Leu Ala Glu Arg Thr Phe Phe Cys
515 520 525
Glu His Cys Gly Met Glu Leu Asp Arg Asp Ile Asn Ala Ala Arg Asn
530 535 540
Ile Leu Ala Phe Ala Gln Ser Ala Tyr Pro Gly Glu Gly Lys Ala Leu
545 550 555 560
Asn Ala Cys Gly Gly Ser Val Ser Pro Gly Ser Gln Ser Val Val Gln
565 570 575
Ala Gly Ala Asp Glu Ala Gly Arg Pro Ala Arg Lys Pro Arg Arg Ser
580 585 590
Ser Arg Gly Ser Asp Pro Pro Ala Thr Pro Thr Thr Arg Ala
595 600 605
<210> 30
<211> 459
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 30
Met Ser Cys Val Thr Lys Thr Gln Val Val Arg Ala Tyr Arg Phe Ala
1 5 10 15
Leu Asp Ala Thr Pro Ser Gln Val Glu Ile Leu Arg Arg Tyr Ala Asn
20 25 30
Ala Ser Arg Ala Ala Phe Asn Phe Ala Leu Gly Met Lys Thr Ala Ser
35 40 45
His Glu Arg Trp Lys Arg Gly Arg Asp Pro Leu Val Ala Ala Asp Met
50 55 60
Asp Met Ala Glu Ala Lys Lys Lys Ala Pro Lys Val Arg Ile Pro Arg
65 70 75 80
Lys Pro Gln Val His Arg His Phe Ile Ala Thr Lys Gly Lys Gly Pro
85 90 95
Ile Gly Pro Leu Arg Gln Gly Glu Glu Arg Arg Thr Pro Tyr Pro Trp
100 105 110
Trp Glu Gly Val Asn Ala Asn Ser Phe Gln Glu Ala Phe Phe Asp Ala
115 120 125
Asp Ala Ala Trp Lys Asn Trp Leu Asp Ser Met Thr Gly Lys Arg Ala
130 135 140
Gly Ala Pro Val Gly Tyr Pro Arg Phe Lys Arg Lys Gly Ser Arg Glu
145 150 155 160
Ser Phe Arg Leu Thr Asn Thr Ala Val Arg Phe Ala Gly Tyr Arg Arg
165 170 175
Leu Trp Ile Gly Gly Gly Gly Gly Gln Ala Ala Phe Thr Val Arg Leu
180 185 190
His Glu Pro Ala Arg Arg Leu Val Arg Leu Leu Asp Ala Gly Gln Ala
195 200 205
Arg Ile Leu Arg Ala Thr Ile Ala Arg Asp Gly His Arg Trp Phe Ala
210 215 220
Ala Val Val Val Glu Glu Ile Ala Val Leu Pro Asn Arg Pro Thr Arg
225 230 235 240
Arg Gln Ala Asn Ala Gly Arg Val Gly Val Asp Leu Gly Val Lys Ser
245 250 255
Ala Ala Ala Phe Ser Asp Pro Val Val Leu Ala His Gly Ala Pro Gly
260 265 270
Val Leu Glu Leu Ala Asn Pro Arg His Val Arg Asn Thr Glu Lys Lys
275 280 285
Leu Ala Arg Ala Gln Arg Val Ala Ser Arg Arg Phe Val Lys Gly Ala
290 295 300
Gln Lys Gln Ser Lys Gly Tyr His Glu Ala Lys Ala Arg Val Ala Lys
305 310 315 320
Leu His Ala Gln Leu Ala Ala Arg Arg Ala Ser Thr Gln His Leu Leu
325 330 335
Thr Arg Arg Leu Val Glu Gln Tyr Ala Glu Val Ala Leu Glu Lys Leu
340 345 350
Met Val Lys Asn Met Thr Arg Ser Ala Lys Gly Ser Ala Glu Asn Pro
355 360 365
Gly Lys Asn Val Arg Gln Lys Ala Gly Leu Asn Arg Ala Ile Leu Asp
370 375 380
Val Gly Phe Gly Glu Ile Arg Arg Gln Ile Glu Tyr Lys Ala Ala Trp
385 390 395 400
Tyr Gly Ser Ser Thr Arg Tyr Val Gly Thr Tyr Phe Pro Ser Ser Lys
405 410 415
Thr Cys Ser Asn Cys His Trp Lys Asn Lys Glu Leu Thr Leu Ala Asp
420 425 430
Arg Val Phe Lys Cys Thr Gln Cys Gly Met Ile Met Asp Arg Asp Ala
435 440 445
Asn Ala Ala Gln Met Ile Lys Lys His Ala Gln
450 455
<210> 31
<211> 517
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 31
Met Gln Gln Glu Ile Leu Lys Ala Phe Arg Phe Ala Leu Asp Pro Thr
1 5 10 15
Pro Ser Gln Leu Glu Gly Leu Ala Gln His Ala Gly Ala Ala Arg Trp
20 25 30
Ala Phe Asn His Ala Leu Gly Met Lys Val Ala Ala His Gln Gln Trp
35 40 45
Arg Ser Ala Val Gln Glu Leu Val Asp Asn Gly Met Ser Glu Ala Gln
50 55 60
Ala Arg Lys Glu Val Arg Val Pro Val Pro Thr Lys Ala Val Val Gln
65 70 75 80
Lys His Leu Asn Gln Ile Lys Gly Asp Ser Arg Gly Asp Ala Leu Pro
85 90 95
Asp Gly Ser Leu Gly Pro Glu Arg Pro Cys Pro Trp Trp His Glu Val
100 105 110
Asn Thr His Ala Phe Gln Ser Ala Phe Ile Asp Ala Asp Arg Ala Trp
115 120 125
Lys Asn Trp Leu Asp Ser Leu Lys Gly Thr Arg Ala Gly Arg Arg Val
130 135 140
Gly Tyr Pro Arg Phe Lys Lys Lys Gly Arg Ala Arg Glu Ala Phe Arg
145 150 155 160
Leu His His Asn Val Lys Gln Pro Thr Val Arg Leu Glu Ser Tyr Arg
165 170 175
Arg Leu Arg Leu Pro Thr Ile Gly Ser Val Arg Leu His Asp Thr Ala
180 185 190
Lys Arg Met Ser Arg Ser Ile Gly Arg Gly Asp Ala Val Ile Gln Ser
195 200 205
Val Thr Val Ser Arg Ala Gly Gln Arg Trp Tyr Ala Ser Val Leu Cys
210 215 220
Lys Val Asn Ala Asp Leu Pro Asp Gln Pro Thr Arg Arg Gln Trp Asp
225 230 235 240
Arg Gly Arg Val Gly Val Asp Leu Gly Val Lys His Leu Ala Val Leu
245 250 255
Ser Gln Pro Leu Glu Thr Asp Ala Ala Thr Thr Ala Phe Val Ser Asn
260 265 270
Pro Arg His Ile Arg Lys Ala Glu Gln Gln Leu Ala Lys Ala Gln Arg
275 280 285
Ala Leu Ser Arg Thr Gln Lys Gly Ser Ala Arg Arg Ala Lys Ala Arg
290 295 300
Arg Arg Val Gly Arg Val His His Glu Val Ala Val Arg Arg Ser Thr
305 310 315 320
Val Leu His Ala Leu Thr Lys Gln Leu Ala Thr Arg Phe Ala Glu Val
325 330 335
Ala Ile Glu Asp Leu His Val Ser Gly Met Thr Arg Ser Ser Ser Gly
340 345 350
Thr Pro Asp Lys Pro Gly Arg His Val Lys Gln Lys Ala Gly Leu Asn
355 360 365
Arg Ala Ile Leu Asp Thr Ala Pro Gly Glu Leu Arg Arg Gln Leu Thr
370 375 380
Tyr Lys Thr Arg Trp Tyr Gly Ser Thr Leu Ala Val Leu Asp Arg Trp
385 390 395 400
Phe Pro Ser Ser Lys Thr Cys Ser Ala Cys Gly Trp Gln Asn Pro Arg
405 410 415
Leu Thr Leu Ala Asp Arg Thr Phe His Cys Thr Asn Cys Ser Thr Thr
420 425 430
Ile Asp Arg Asp Leu Asn Ala Ala Arg Asn Ile Ala Gln His Ala Val
435 440 445
Leu Ala Asp Gln Leu Leu Ala Pro Gly Arg Gly Glu Thr Gln Asn Ala
450 455 460
Arg Gly Ala Pro Ile Arg Pro Pro Gly Pro Arg Ala Gly Gly Gln Glu
465 470 475 480
Ala Met Lys Arg Glu Asp Thr Ser Pro Ala Arg Pro Val Pro Pro Gln
485 490 495
Arg Ser Asp Pro Leu Thr Leu Phe Thr Leu Asp Asp Thr Gly His Glu
500 505 510
Thr Ala Lys Arg Ala
515
<210> 32
<211> 469
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 32
Met Ser Glu Thr Val Thr Ile Val Val Lys Glu Ala Leu Asp Pro Thr
1 5 10 15
Pro Ala Gln Val Val Ile Leu Gln Arg Tyr Ala Asp Ala Ser Arg Cys
20 25 30
Ser Phe Asn Tyr Ala Leu Gly Leu Lys Arg Gly Ala Gln Gln Thr Trp
35 40 45
Ser Gln Gly Arg Asp Arg Leu Val Ala Gln Gly Gln Thr Pro Ala Glu
50 55 60
Ala Ala Arg Asn Ala Pro Lys Val Arg Ile Pro Gly Gln Phe Asp Ile
65 70 75 80
Gln Lys Ile Phe Leu Ala Lys Arg Asp Glu Pro Leu Pro Gly Pro Leu
85 90 95
Leu Pro Gly Gln Glu Pro Arg Leu Leu Tyr Pro Trp Trp Lys Gly Val
100 105 110
Asn Ala Ile Val Cys Gln Gln Ala Phe Arg Asp Ala Asp Ala Ala Phe
115 120 125
Ala Asn Trp Lys Ser Ser Ala Arg Arg Lys Gly Gly Pro Val Gly Phe
130 135 140
Pro Arg Phe Lys Arg Arg Gly Arg Cys Arg Asp Ser Phe Arg Met Phe
145 150 155 160
Ser Ile Arg Leu Val Glu Glu Asp Leu Arg His Val Arg Ile Gly Gly
165 170 175
Glu Arg Gly Gly Gln Pro Ala Phe Thr Val Arg Leu His Arg Pro Ala
180 185 190
Arg Arg Leu Ala Arg Leu Leu Ala Glu Gly Gly Glu Thr Lys Ser Val
195 200 205
Thr Val Ala Arg Glu Gly His Arg Trp Phe Val Ala Phe Asn Val Arg
210 215 220
Val Pro Ala Gly Pro Pro Pro Arg Pro Thr Arg Arg Gln Arg Gly Ala
225 230 235 240
Gly Thr Val Gly Val Asp Leu Gly Val Lys Val Phe Ala Ala Thr Ser
245 250 255
Asp Pro Leu Leu Ile Asn Gly Thr Gly Leu Gln Leu Phe Glu Asn Pro
260 265 270
Arg Leu Leu Asp Asn Ala Arg Arg Gln Leu Arg Lys Trp Gln Arg Arg
275 280 285
Met Ala Arg Arg His Val Arg Gly Leu Arg Ala Asp Glu Gln Ser Ser
290 295 300
Gly Trp Lys Glu Ala Arg Asp Gln Val Ala Arg Leu His Ala Leu Val
305 310 315 320
Ala Ala Arg Arg Ser Ser Thr Gln His Leu Leu Thr Lys Arg Ile Val
325 330 335
Thr Gln Tyr Glu His Val Ala Leu Glu Asp Leu Arg Val Lys Asn Met
340 345 350
Thr Ala Ser Ala Arg Gly Thr Ala Gln Ser Pro Gly Arg Asn Val Lys
355 360 365
Ala Lys Ala Gly Leu Asn Arg Ala Ile Leu Asp Val Gly Phe Gly Glu
370 375 380
Ile Arg Arg Gln Ile Glu Tyr Lys Ala Leu Leu Tyr Gly Thr Thr Val
385 390 395 400
Thr Val Val Asp Pro Ala Tyr Thr Ser Gln Thr Cys Asn Arg Cys Gly
405 410 415
His Val Asp Ala Lys Ser Arg Arg Thr Arg Asp Leu Phe Thr Cys Thr
420 425 430
Arg Cys Gly His Ala Thr His Ala Asp Ile Gly Ala Ala Ile Asn Ile
435 440 445
Lys Ala Arg Ala Gln His Ser His Pro Ala Gln Glu Arg Pro Glu Gly
450 455 460
Thr Thr Asp Val Pro
465
<210> 33
<211> 583
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 33
Met Phe Glu Arg Thr Thr Thr Lys Ala Ala Phe Leu Ser Pro Leu Asp
1 5 10 15
Leu Arg Pro Thr Gln Ala Thr Asp Leu Glu Arg Phe Ala Gly Thr Thr
20 25 30
Arg Trp Ala Phe Asn Trp Ala Asn Ala Leu Leu Glu Ala His His Gln
35 40 45
Ala Tyr Glu Gly Arg Arg Gln Gln Ala Ala Arg His Leu Phe Gly Leu
50 55 60
Gly Pro Glu Gln Leu Asp Glu Leu Arg Val Leu Ala Asn Gly Thr Arg
65 70 75 80
Asp Glu Asn Gly Lys Lys Ala Lys Gly Asp Pro Val Lys Arg Arg Glu
85 90 95
Tyr Glu Ser Ile Gln Lys Ala Thr Lys Lys Ala Val Ser Glu Glu Asn
100 105 110
Lys Ala Leu Gly Ala Glu Met Lys Leu Trp Asp Glu His Arg Ser Leu
115 120 125
Val Val His Lys Gly Arg Pro Leu Leu Thr Pro Gly Asp Glu Pro Ala
130 135 140
Leu Asp Ala Pro Pro Leu Ala His Arg Leu Tyr Ala Arg Arg Val Glu
145 150 155 160
Leu Ala Gly Ile Gln Lys Thr Asp Pro Asp Tyr Tyr Ala Glu Gln Arg
165 170 175
Lys Lys Glu Arg Glu Ala Ile Thr Pro Asn Val Val Ala Met Lys Arg
180 185 190
Asp Leu Met Ala Lys Gly Ala Tyr Phe Pro Ser Glu Tyr Asp Leu Gln
195 200 205
Tyr Ile Trp Arg Thr Val Arg Asp Leu Pro Lys Glu Glu Gly Gly Ser
210 215 220
Pro Trp Trp Pro Glu Cys Pro Thr Ile Leu Phe Tyr Asp Gly Ile Asn
225 230 235 240
Arg Ala Arg Thr Ala Trp Lys Asn Trp Met Asp Ser Ala Ser Gly Ala
245 250 255
Arg Lys Gly Pro Pro Val Gly Met Pro Arg Phe Lys Ser Lys Tyr Lys
260 265 270
Ala Lys Asp Thr Phe Thr Ile Thr Asn Pro Asn Arg Ser Val Ile Lys
275 280 285
Phe Glu Thr Tyr Arg Arg Ile Ala Ile Thr Gly Ile Gly Ser Met Arg
290 295 300
Leu His Arg Gly Ala Lys Leu Leu Ala Arg Arg Ile Ala Ala Gly Gln
305 310 315 320
Ala Glu Ile Thr Ser Ala Thr Ile Ser Arg Ser Gly Thr Ala Trp Tyr
325 330 335
Val Ser Val Leu Cys Thr Val His Thr Thr Ala Arg Thr Ala Pro Ser
340 345 350
Lys Ala Gln Arg Ser Arg Gly Ala Val Gly Val Asp Trp Gly Val Arg
355 360 365
Ala Leu Ala Thr Thr Ser Lys Pro Ile Ala Leu Thr Pro Gly Lys Pro
370 375 380
Ala Ser Arg Thr Val Pro Ala Glu Lys Tyr Gly Ala Ala Met Ser Gln
385 390 395 400
Lys Ile Ala Arg Ala Gln Arg Gln Leu Ala Arg Met Pro Lys Gly Ser
405 410 415
Ser Arg Arg Arg Lys Ala Ala Arg His Val Ala Asp Leu Gln His Leu
420 425 430
Val Ala Gln Arg Arg Ala Ser Ser Val His Gln Leu Ser Lys Ala Leu
435 440 445
Ala Gln Ser Phe Glu Ile Val Ala Ile Glu Gly Leu Asn Val Arg Gly
450 455 460
Met Thr Lys Ser Ala Lys Gly Thr Val Glu Asn Pro Gly Lys Asn Ile
465 470 475 480
Arg Gln Lys Ala Gly Leu Asn Arg Ala Ile Leu Asp Ala Thr Pro Gly
485 490 495
Glu Leu Lys Arg Gln Leu Glu Tyr Lys Thr Lys Lys Tyr Gly Ser Arg
500 505 510
Leu Val Glu Leu Asp Thr Trp Tyr Pro Ser Ser Lys Thr Cys Ser Arg
515 520 525
Cys Gly Trp Val His Pro Lys Leu Lys Leu Ser Met Arg Thr Phe Arg
530 535 540
Cys Gln Gln Cys Gly Leu Val Glu Asp Arg Asp Phe Asn Ala Ala Val
545 550 555 560
Asn Ile Glu Arg Gln Gly Ile Thr His Ile Val Lys Glu Asn Glu Gly
565 570 575
Thr Asp Asp Arg Glu Glu Gly
580
<210> 34
<211> 466
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 34
Met Asp Glu Ala Leu Ser Pro Arg Gln Val Thr Arg Val Val Arg Leu
1 5 10 15
Pro Leu Asp Pro Thr Pro Val Glu Gln Ala Ile Leu Gln Arg Tyr Ala
20 25 30
Asp Cys Ser Arg Ala Cys Tyr Asn Phe Ala Phe Ser Leu Lys Asp Ala
35 40 45
Ala Gln Arg Arg Trp Ala Ala Glu Arg Asp Arg Leu Ile Ala Asp Gly
50 55 60
Met Gln Glu Lys Ala Ala Arg Lys Ala Ala Thr Ala Arg Phe Ala Val
65 70 75 80
Pro Arg Gln Phe Asp Leu Gln Lys Ile Phe Leu Ala Val Arg Asp Arg
85 90 95
Pro Phe Thr Gly Pro Glu Arg Ala Gly Glu Pro Phe Pro Arg Tyr Arg
100 105 110
Tyr Arg Trp Trp Ala Gly Val Asn Ala Met Val Cys Gln Gln Ala Phe
115 120 125
Arg Asp Ala Asp Arg Ala Trp Ser Asn Trp Leu Ser Arg Ser Arg Glu
130 135 140
Gly His Gly Tyr Pro Arg Pro Lys Gln Arg Gly Arg Cys Lys Asp Ser
145 150 155 160
Phe Arg Leu Pro Gly Val Ser Leu Ala Ala Glu Asp Leu Arg His Ile
165 170 175
Arg Ile Ser Gly Glu Arg Arg Pro Gly Gly Gln Lys Ala Phe Arg Val
180 185 190
Arg Leu His Arg Pro Ala Tyr Arg Leu Ala Arg Leu Leu Gln Gln Gly
195 200 205
Gly Gln Val Lys Met Val Thr Val Ser Arg Ser Gly Pro Arg Trp Phe
210 215 220
Ala Ala Phe Asn Val Arg Leu Pro Ala Pro Pro Ala Val Val Ala Thr
225 230 235 240
Arg Ala Gln Arg Arg Arg Gly Ala Val Gly Val Asp Leu Gly Val Glu
245 250 255
Val Ile Ala Ala Thr Ser Gln Pro Val Leu Leu Gln Gly Ala Val Thr
260 265 270
Gln Leu Val Ala Asn Pro Arg Tyr Leu Ala Asn Ala Arg Arg Ala Leu
275 280 285
Ala Lys Trp Glu Arg Arg Lys Ala Arg Arg Trp Val Lys Gly Leu Pro
290 295 300
Ala Gly Gln Gln Ser Arg Gly Trp His Glu Ala Lys Asp His Val Ala
305 310 315 320
Lys Leu His Ala Leu Ile Ala Ala His Arg Ala Ser Thr Gln His His
325 330 335
Leu Thr Lys Ala Leu Val Thr Gln Phe Ala Gln Val Val Ile Glu Asp
340 345 350
Leu Gln Val Lys Asn Met Ser Lys Ser Ala Lys Gly Thr Leu Glu Asp
355 360 365
Pro Gly Ser Arg Val Arg Gln Lys Ala Gly Leu Asn Arg Ala Leu Leu
370 375 380
Asp Val Gly Phe Gly Glu Ile Arg Arg Gln Leu Ala Tyr Lys Ala Pro
385 390 395 400
Trp Tyr Gly Ser Val Leu Ser Ala Val Asn Pro Ala Tyr Thr Ser Gln
405 410 415
Thr Cys His Arg Cys Gly His Val Asp Ser Lys Ser Arg Arg Thr Arg
420 425 430
Ser Val Phe Glu Cys Thr Ala Cys Gly Thr Gln Val His Ala Asp Ile
435 440 445
Gly Ala Ala His Asn Ile Leu Ala Arg Gly Gln Gln Thr Pro Ala Ala
450 455 460
Leu Phe
465
<210> 35
<211> 463
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 35
Met Thr Glu Asn Thr Val Thr Val Val Val Arg Glu Ala Leu Asp Pro
1 5 10 15
Thr Pro Glu Gln Arg Leu Ile Leu His Arg Tyr Ala Asp Ala Ser Arg
20 25 30
Cys Ser Phe Asn Phe Ala Phe Gly Ile Lys His Ala Ala Gln Gln Arg
35 40 45
Trp Ser His Gly Arg Asp Leu Leu Leu Ala Gln Gly Leu Thr Ser Ala
50 55 60
Glu Ala Ala Lys Arg Ala Pro Arg Val His Met Pro Ser Gln Pro Asp
65 70 75 80
Ile Gln Arg Val Phe Leu Ala Val Arg Glu Arg Pro Leu Pro Gly Pro
85 90 95
Cys Arg Val Gly Glu Glu Pro His Ser Leu Tyr Pro Trp Trp Lys Gly
100 105 110
Val Asn Ala Ile Val Cys Gln Gln Ala Phe Arg Asp Ala Asp Lys Ala
115 120 125
Phe Ala Asn Trp Lys Ser Ala Ser Arg Arg Gly Ala Gly Pro Val Gly
130 135 140
Tyr Pro Arg Pro Lys Arg Arg Gly Arg Cys Arg Asp Ser Phe Arg Met
145 150 155 160
Phe Ser Val Arg Leu Val Ser Glu Asp Leu Arg His Val Arg Ile Gly
165 170 175
Gly Glu Arg Asp Pro His Gly Gln Arg Ser Phe Val Val Arg Leu His
180 185 190
Arg Pro Ala Arg Arg Leu Ala Arg Leu Leu Ala Arg Gly Gly Val Ala
195 200 205
Lys Ser Val Thr Val Ala Arg Glu Gly His Arg Trp Phe Ala Ala Phe
210 215 220
Asn Val Arg Leu Pro Ala Pro Pro Ala Val Arg Ala Thr Arg Arg Gln
225 230 235 240
Arg Glu Ala Gly Thr Val Gly Val Asp Leu Gly Val Ala Val Phe Ala
245 250 255
Ala Thr Ser Asp Pro Leu Val Ile Gly Asp Asp Lys Val Gln Leu Phe
260 265 270
Ala Asn Pro Arg His Leu Asp Asn Ala Arg Arg Gln Leu Arg Lys Trp
275 280 285
Gln Arg Arg Met Ser Arg Arg His Val Arg Gly Val Pro Ala His Arg
290 295 300
Gln Ser Lys Gly Trp Lys Glu Ala Arg Asp Gln Val Ala Arg Leu Gln
305 310 315 320
Gly Leu Val Ala Ala Arg Arg Ser Ser Thr Gln His Leu Leu Thr Lys
325 330 335
Arg Leu Val Thr Gln Tyr Ala Gln Val Val Leu Glu Asp Leu Arg Val
340 345 350
Arg Asn Met Thr Ser Ser Ala Arg Gly Ser Val Glu Ala Pro Gly Arg
355 360 365
Asn Val Val Ala Lys Ala Gly Leu Asn Arg Ala Ile Leu Asp Val Gly
370 375 380
Phe Gly Glu Ile Arg Arg Gln Ile Glu Tyr Lys Ala Pro Trp His Gly
385 390 395 400
Thr Thr Val Ser Val Val Asn Pro Ala Tyr Thr Ser Gln Thr Cys Asn
405 410 415
Arg Cys Gly His Thr Asp Ala Lys Ser Arg Arg Thr Arg Ser Leu Phe
420 425 430
Val Cys Thr Arg Cys Arg His Thr Thr His Ala Asp Thr Gly Ala Ala
435 440 445
Val Asn Ile Lys Arg Arg Ala Gln Ser Ala Ala Pro Lys Pro Gly
450 455 460
<210> 36
<211> 511
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 36
Met Gly Arg Ala Glu Val Leu Arg Ala Phe Arg Phe Ala Leu Asp Pro
1 5 10 15
Thr Asp Thr Gln Leu Ala Ser Leu Asn Gln His Ala Gly Ala Ala Arg
20 25 30
Trp Ala Tyr Asn His Ala Ile Gly Val Lys Ser Gly Ala His Ala Arg
35 40 45
Arg Arg Ala Glu Val Glu Ala Leu Val Ala Ala Gly Met Thr Glu Lys
50 55 60
Asp Ala Arg Lys Ala Val Asn Val Pro Ile Pro Met Lys Pro Ala Ile
65 70 75 80
Gln Thr Ser Leu Asn Ala Ile Lys Gly Asp Pro Arg Val Gln Glu Val
85 90 95
Leu Pro Gly Ala Met Gly Pro His Arg Pro Cys Pro Trp Trp Arg Asp
100 105 110
Val Ala Thr His Ala Phe Gln Ser Ala Phe Arg Asp Ala Asp Thr Ala
115 120 125
Phe Lys Asn Trp Met Asp Ser Leu Arg Gly Gln Arg Ala Gly Arg Pro
130 135 140
Val Gly Tyr Pro Arg Phe Lys Arg Lys Gly Arg Ala Arg Asp Ser Phe
145 150 155 160
Arg Leu His His Asp Val Lys Ala Pro Thr Ile Arg Leu Asp Gly Tyr
165 170 175
Arg Arg Leu Leu Leu Pro Arg Ile Gly Ser Val Arg Leu His Glu Ser
180 185 190
Gly Lys Arg Leu Ala Arg Leu Val Gly Arg Gly Glu Ala Val Val Gln
195 200 205
Ser Val Thr Val Ala Arg Gly Gly Ser Arg Trp Tyr Ala Ser Val Leu
210 215 220
Cys Lys Val Trp Gln Glu Leu Pro Glu Gln Pro Thr His Ala Gln Arg
225 230 235 240
Ala Arg Gly Thr Ile Gly Val Asp Leu Gly Val Lys Val Leu Ala Ala
245 250 255
Val Ser Lys Pro Val Ser Met Thr Arg Gly Gly Pro Gln Leu Asp Leu
260 265 270
Val Pro Asn Ala Arg His Gly Lys Ala Ala Arg Arg Arg Leu Thr Arg
275 280 285
Ala Gln Gln Thr Tyr Ala Arg Thr Ala Lys Gly Ser Lys Arg Arg Ala
290 295 300
Lys Ala Ala Arg Arg Ile Gly Arg Ile Gln His Leu Thr Ala Glu Arg
305 310 315 320
Arg Ala Thr Ser Leu His Thr Leu Thr Lys Arg Leu Val Thr Ala Phe
325 330 335
Ala Thr Val Ala Val Glu Asp Leu Asn Val Ala Gly Met Thr Ala Ser
340 345 350
Ala Arg Gly Thr Val Asp Asn Pro Gly Arg Lys Val Arg Gln Lys Ala
355 360 365
Gly Leu Asn Arg Ser Val Leu Asp Ala Ser Phe Gly Glu Leu Arg Arg
370 375 380
Gln Leu Thr Tyr Lys Ala His Trp Tyr Gly Ala Ala Ile Ala Val Cys
385 390 395 400
Gly Arg Trp Val Pro Ser Ser Gln Thr Cys Ser Val Cys Gly Trp Gln
405 410 415
Asn Pro Arg Arg Leu Thr Leu Ala Asp Arg Glu Phe Glu Cys Thr Pro
420 425 430
Cys Gly Leu Thr Met Asp Arg Asp Leu Asn Ala Ala Arg Asn Ile Glu
435 440 445
Arg Leu Ala Gln His Val Ala Ser Gly Arg Glu Glu Thr Glu Asn Ala
450 455 460
Arg Gly Gly Gly Val Ser Pro Pro Val Arg Leu Gly Gly Arg Gln Ser
465 470 475 480
Pro Val Lys Arg Glu Asp Pro Ala His Arg Ala Gly Pro Pro Arg Arg
485 490 495
Ser Asp Pro Pro Ala Thr Leu Thr Ala Arg Arg Lys Arg Ala Ala
500 505 510
<210> 37
<211> 519
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 37
Met Ala Glu Thr Ala Val Leu Arg Ala Phe Arg Phe Ala Leu Asp Ala
1 5 10 15
Thr Ala Ala Gln Glu Glu Gly Phe Leu Arg His Ala Gly Ala Ser Arg
20 25 30
Trp Ala Phe Asn His Ala Leu Gly Met Lys Val Ala Ala His Arg Gln
35 40 45
Trp Gln Arg Glu Val Lys Ala Leu Val Glu Gly Gly Met Leu Glu Ala
50 55 60
Gln Ala Arg Lys Thr Val Lys Val Pro Val Pro Thr Arg Pro Thr Ile
65 70 75 80
Gln Lys His Leu Asn Arg Ile Lys Gly Asp Ser Arg Ser Pro Asp His
85 90 95
Pro Glu Gly Ala Gln Gly Pro Gln Arg Pro Cys Pro Trp Phe His Glu
100 105 110
Val Ser Thr Tyr Ala Phe Gln Cys Ala Phe Glu Asp Ala Asp Arg Ala
115 120 125
Trp Asp Asn Trp Gln Ala Ser Leu Ser Gly Arg Arg Ala Gly Arg Lys
130 135 140
Val Gly Tyr Pro Arg Phe Lys Lys Lys Gly Arg Thr Lys Asp Ser Phe
145 150 155 160
Arg Ile Cys His Asp Ala Lys Lys Pro Thr Ile Arg Pro Asp Gly Tyr
165 170 175
Arg Arg Leu Arg Ile Pro Val Leu Gly Ser Val Arg Leu His Asp Thr
180 185 190
Ala Lys Pro Leu Ala Arg Leu Val Asp Arg Gly Ala Ser Val Lys Ser
195 200 205
Val Thr Val Ser Arg Ser Gly Ala Arg Trp Tyr Ala Ser Val Leu Val
210 215 220
Ser Val Leu Gln Asp Leu Pro Glu Arg Pro Thr Arg Arg Gln Arg Gln
225 230 235 240
Ala Gly Thr Val Gly Val Asp Phe Gly Val Lys Thr Leu Ala Ala Leu
245 250 255
Ser Ala Pro Val Thr Leu Pro Asn Leu Gly Thr Leu Thr Met Val Pro
260 265 270
Asn Pro Arg His Leu Ala Ser Asp Thr Arg Arg Leu Thr Lys Ala Gln
275 280 285
Gln Thr Leu Ser Arg Thr Thr Lys Gly Ser Ala Arg Arg Arg Lys Ala
290 295 300
Ala Gln Arg Val Gly Ile Val His His Arg Ile Ala Ser Arg Arg Ala
305 310 315 320
Thr Tyr Leu His Thr Leu Thr Lys Gln Leu Ala Thr Gly Tyr Ala Val
325 330 335
Val Ala Val Glu His Leu Asn Val Ala Gly Met Thr Ser Ser Ala Arg
340 345 350
Gly Thr Val Glu Glu Pro Gly Ser Lys Val Arg Gln Lys Ser Gly Leu
355 360 365
Asn Arg Ser Ile Leu Asp Ala Ser Pro Ala Glu Met Arg Arg Gln Leu
370 375 380
Asp Tyr Lys Thr Arg Trp Asn Gly Ser Gln Leu Ala Val Cys Asp Arg
385 390 395 400
Trp Phe Pro Ser Ser Arg Thr Cys Ser Ala Cys Gly Trp Gln Lys Pro
405 410 415
Arg Leu Thr Leu Ala Glu Arg Val Phe Asn Cys Gly Gln Cys Gly Leu
420 425 430
Val Ile Asp Arg Asp Leu Asn Ala Ala Arg Asn Ile Ala Ala His Ala
435 440 445
Val Leu Val Pro His Gly Thr Ala Ala Pro Gly Ser Gly Glu Ala Ser
450 455 460
Asn Ala Arg Gly Ala Ala Thr Arg Pro Ala Thr Pro Arg Gly Gly Arg
465 470 475 480
Gln Ala Ala Leu Lys Arg Glu Asp Thr Gly Pro Pro Arg Pro Val Pro
485 490 495
Pro Gln Arg Ser Asp Pro Leu Thr Leu Phe Thr Leu Asp Val Pro Asp
500 505 510
Gln Gln Thr Ala Lys Arg Pro
515
<210> 38
<211> 515
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 38
Met Glu Gln Glu Val Leu Lys Ala Phe Arg Phe Ala Leu Asp Pro Arg
1 5 10 15
Pro Ala Gln Val Glu Val Leu Leu Arg His Ala Gly Ala Ala Arg Trp
20 25 30
Ala Phe Asn Leu Ala Leu Gly Met Lys Val Ala Ala His Lys Glu Trp
35 40 45
Arg Ser Gln Val Gln Ala Leu Val Asp Gln Gly Val Pro Glu Ala Glu
50 55 60
Ala Arg Arg Arg Val Lys Val Pro Val Pro Ser Lys Leu Gln Ile Gln
65 70 75 80
Lys His Leu Asn Leu Ile Lys Gly Asp Ser Arg Glu Gly Gly Leu Pro
85 90 95
Glu Gly Val Leu Gly Pro Glu Arg Pro Cys Pro Trp Trp His Glu Val
100 105 110
Ser Thr Tyr Ala Phe Gln Ser Ala Phe Ile Asp Ala Asp Arg Ala Trp
115 120 125
Gln Asn Trp Leu Ala Ser Leu Ala Gly Lys Arg Ala Gly Arg Ala Val
130 135 140
Gly Tyr Pro Arg Phe Lys Lys Lys Gly Arg Cys Arg Asp Ala Phe Arg
145 150 155 160
Leu His His Asp Val Lys Arg Pro Gly Ile Arg Pro Val Gly Tyr Arg
165 170 175
Arg Leu Arg Leu Pro Thr Val Gly Glu Val Arg Leu His Gly Ser Ala
180 185 190
Lys Arg Leu Val Arg Leu Leu Gly Arg Gly Cys Ala Gln Val Gln Ser
195 200 205
Val Thr Val Ser Arg Gly Gly His Arg Trp Tyr Ala Ser Val Leu Cys
210 215 220
Lys Val Val Val Glu Leu Pro Gly Lys Pro Thr Lys Ala Gln Ala Arg
225 230 235 240
Arg Gly Thr Ile Gly Val Asp Leu Gly Val Lys Tyr Leu Ala Ala Leu
245 250 255
Ser Gln Pro Leu Asp Val Lys Val Pro Glu Ser Arg Phe Val Asp Asn
260 265 270
Pro Arg His Leu Val Arg Ala Glu Lys Gln Leu Ala Lys Ala Gln Arg
275 280 285
Ala Leu Ser Arg Thr Gln Lys Gly Ser Ala Arg Arg His Lys Ala Arg
290 295 300
Cys Arg Val Gly Arg Leu His His Glu Val Ala Val Arg Arg Ala Thr
305 310 315 320
Val Leu His Ala Met Thr Lys Gln Leu Ala Thr Arg Phe Ser Thr Val
325 330 335
Ala Val Glu Asp Leu Tyr Val Ala Gly Met Thr Arg Ser Ala Arg Gly
340 345 350
Thr Val Glu Ala Pro Gly Lys Arg Val Arg Gln Lys Ala Gly Leu Asn
355 360 365
Arg Ala Ile Leu Asp Ala Ala Pro Gly Glu Val Arg Arg Gln Leu Ala
370 375 380
Tyr Lys Thr Arg Trp Tyr Gly Ser Lys Leu Ala Val Leu Asp Arg Trp
385 390 395 400
Phe Pro Ser Ser Lys Thr Cys Phe Ala Cys Gly Trp Gln Asn Pro Arg
405 410 415
Leu Thr Leu Ala Asp Arg Thr Phe His Cys Gly Gly Cys Gly Leu Ser
420 425 430
Thr Asp Arg Asp Glu Asn Ala Ala Arg Asn Ile Ala His His Ala Val
435 440 445
Val Ala Asp Asp Ser Pro Val Ala Pro Gly Lys Glu Glu Thr Arg Asn
450 455 460
Ala Arg Gly Ala Pro Asn Leu Ala Gly Leu Lys Ala Arg Arg Gln Gly
465 470 475 480
Ala Ser Lys Arg Glu Gly Thr Gly Pro Pro Gly Thr Val Pro Pro Gln
485 490 495
Arg Ser Asn Pro Leu Thr Leu Pro Asp Pro His Gly Gln Gln Pro Ala
500 505 510
Lys His Pro
515
<210> 39
<211> 518
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 39
Met Glu Gln Gln Val Leu Lys Ala Phe Lys Phe Ala Leu Asp Pro Thr
1 5 10 15
Ser Ser Gln Val Glu Glu Leu Thr Arg His Ala Gly Ala Ser Arg Trp
20 25 30
Ala Phe Asn His Ala Leu Gly Met Lys Ile Ala Ala His Arg Asp Trp
35 40 45
Arg Thr Gln Val Gln Glu Leu Ile Asp Ala Gly Val Gln Glu Lys Ala
50 55 60
Ala Arg Gln Gln Val Arg Val Pro Ala Pro Thr Lys Pro Thr Ile Gln
65 70 75 80
Lys His Leu Asn Ser Ile Lys Gly Asp Ser Arg Ser Asp Asp Leu Pro
85 90 95
Pro Gly Ala Met Gly Pro Gln Arg Pro Cys Pro Trp Trp His Glu Val
100 105 110
Asn Thr His Ala Phe Gln Ser Ala Phe Ile Asp Ala Asp Thr Ala Trp
115 120 125
Lys Asn Trp Leu Asp Ser Leu Arg Gly Thr Arg Ala Gly Arg Lys Val
130 135 140
Gly Tyr Pro Arg Phe Lys Lys Lys Gly Arg Ser Arg Asp Ser Phe Arg
145 150 155 160
Leu His His Asp Val Asn Lys Pro Gly Ile Arg Leu Ala Thr Tyr Arg
165 170 175
Arg Leu Arg Leu Pro Lys Ile Gly Glu Val Arg Leu His Asp Ser Gly
180 185 190
Lys Arg Leu Ala Arg Leu Ile Gly Arg Gly Asp Ala Val Ile Gln Ser
195 200 205
Val Thr Val Ser Arg Ala Gly His Arg Trp Tyr Ala Ser Val Leu Ala
210 215 220
Lys Val Thr Val Thr Leu Pro Thr Arg Pro Thr Ala Arg Gln Val Ser
225 230 235 240
Ala Gly Lys Val Gly Val Asp Leu Gly Val Lys Ser Leu Ala Val Leu
245 250 255
Ser Arg Pro Leu Val Pro Gly Asp Asp Thr Thr Ala Phe Val Pro Asn
260 265 270
Pro Arg His Leu Arg His Ala Glu Thr Arg Leu Thr Lys Ala Gln Arg
275 280 285
Ala Leu Ser Arg Thr Thr Lys Gly Ser Ala Arg Arg Glu Lys Ala Arg
290 295 300
Arg Arg Val Ala Arg Leu His His Glu Val Ser Val Arg Arg Ala Gly
305 310 315 320
Gln Leu His Ala Leu Thr Lys Leu Leu Thr Thr Thr Phe Ser Glu Val
325 330 335
Ala Val Glu Asp Leu Asn Val Ala Gly Met Thr Arg Ser Ala Arg Gly
340 345 350
Thr Thr Glu Arg Pro Gly Arg Arg Val Arg Gln Lys Ala Gly Leu Asn
355 360 365
Arg Ala Ile Leu Asp Thr Ser Pro Gly Glu Leu Arg Arg Gln Leu Thr
370 375 380
Tyr Lys Ala Ser Trp Tyr Gly Ser Lys Leu Leu Val Leu Asp Arg Trp
385 390 395 400
Tyr Pro Ser Ser Lys Thr Cys Ser Ala Cys Gly Trp Gln Asn Pro Arg
405 410 415
Leu Thr Leu Ala Asp Arg Thr Phe His Cys Gly Asn Cys His Leu Val
420 425 430
Ile Asp Arg Asp Leu Asn Ala Ala Arg Asn Ile Ala Gln Gln Thr His
435 440 445
Gly Ala Pro His His Pro Val Ala Pro Gly Lys Gly Glu Thr Gln Asn
450 455 460
Ala Arg Arg Ala Thr Gly Arg Pro Pro Ser Pro Arg Ala Gly Arg Gln
465 470 475 480
Glu Ala Gln Lys Arg Glu Asp Thr Gly Pro Pro Arg Ser Val Pro Pro
485 490 495
Gln Arg Ser Asp Pro Leu Thr Ser Pro Pro Pro Gly Ser Pro Gly Gln
500 505 510
Glu Ser Ala Lys Ser Leu
515
<210> 40
<211> 532
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 40
Met Gly Gln Gln Val Leu Lys Ala Phe Lys Phe Ala Leu Asp Pro Thr
1 5 10 15
Pro Gly Gln Thr Glu Glu Leu Thr Arg His Val Gly Ala Ser Arg Trp
20 25 30
Ala Tyr Asn His Ala Leu Gly Met Lys Ile Ala Ala His Arg Glu Trp
35 40 45
Arg Thr Gln Val Gln Ser Leu Val Glu Glu Gly Leu Pro Glu Lys Asp
50 55 60
Ala Arg Gln Arg Val Arg Val Pro Val Pro Thr Lys Pro Thr Ile Gln
65 70 75 80
Lys His Leu Asn Ser Ile Lys Gly Asp Ser Arg Ala Asp Asp Leu Pro
85 90 95
Pro Arg Ala Leu Gly Pro His Arg Pro Cys Pro Trp Trp His Glu Val
100 105 110
Asn Thr Tyr Ala Phe Gln Ser Ala Phe Ile Asp Ala Asp Thr Ala Trp
115 120 125
Lys Asn Trp Leu Asp Ser Leu Arg Gly Ala Arg Ala Gly Arg Lys Val
130 135 140
Gly Tyr Pro Arg Phe Lys Lys Lys Gly Arg Ser Arg Asp Ser Phe Arg
145 150 155 160
Leu His His Asp Val Asn Lys Pro Gly Ile Arg Leu Ala Thr His Arg
165 170 175
Arg Leu Arg Leu Pro Lys Ile Gly Glu Val Arg Leu His Asp Ser Gly
180 185 190
Lys Arg Leu Ala Arg Leu Ile Gly Arg Gly Asp Ala Val Val Gln Ser
195 200 205
Val Thr Val Ser Arg Ala Gly His Arg Trp Tyr Ala Ser Val Leu Ala
210 215 220
Lys Val Thr Val Thr Leu Pro Glu Arg Pro Thr Ala Arg Gln Thr Ser
225 230 235 240
Ala Gly Lys Val Gly Val Asp Leu Gly Val Lys Asn Leu Ala Val Leu
245 250 255
Ser Arg Pro Leu Leu Pro Gly Asp Asp Thr Thr Ala Phe Val Pro Asn
260 265 270
Pro Arg His Leu Arg His Ala Glu Ala Arg Leu Thr Lys Ala Gln Arg
275 280 285
Ala Leu Ser Arg Thr Thr Lys Gly Ser Ala Arg Arg Glu Lys Ala Arg
290 295 300
Arg Arg Val Ala Arg Leu His His Glu Val Ser Val Arg Arg Asp Gly
305 310 315 320
Gln Leu His Ala Leu Thr Lys Leu Leu Thr Thr Ser Phe Ser Glu Val
325 330 335
Ala Val Glu Asp Leu Asn Val Ala Gly Met Thr Arg Ser Ala Arg Gly
340 345 350
Thr Ile Glu Arg Pro Gly Arg Arg Val Arg Gln Lys Ala Gly Leu Asn
355 360 365
Arg Ala Ile Leu Asp Ala Ser Pro Gly Glu Leu Arg Arg Gln Leu Thr
370 375 380
Tyr Lys Ala Ser Trp Tyr Gly Ser Lys Leu Leu Val Leu Asp Arg Trp
385 390 395 400
Tyr Pro Ser Ser Lys Thr Cys Ser Ala Cys Gly Trp Gln Asn Pro Arg
405 410 415
Leu Thr Leu Ala Asn Arg Thr Phe His Cys Ala Asn Cys His Leu Ala
420 425 430
Ile Asp Arg Asp Leu Asn Ala Ala Arg Asn Ile Ala Gln Gln Thr His
435 440 445
Gly Ala Pro His His Pro Val Pro Pro Gly Arg Gly Asp Ala Lys Arg
450 455 460
Pro Pro Ser His Arg Lys Thr Ala Gln Pro Thr Gly Trp Thr Ala Gly
465 470 475 480
Gly Ala Glu Ala Gly Arg His Arg Pro Thr Gln Val Gly Ala Thr Ser
485 490 495
Ala Glu Arg Ser Ala Asp Ile Pro Thr Thr Arg Ala Thr Trp Ser Arg
500 505 510
Lys Gly Lys Ala Gly Leu Thr Ser Gln Ala Arg Lys Cys Pro Ala Arg
515 520 525
Thr Cys Arg Gly
530
<210> 41
<211> 513
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 41
Met Ser Ser Glu Arg Ala Pro Lys Leu Arg Asn Val Val Thr Gln Gln
1 5 10 15
Ala Tyr Lys Tyr Ala Leu Glu Pro Thr Pro Arg Gln Gln Cys Ala Phe
20 25 30
Ser Ser His Ala Gly Ala Ala Arg Phe Ala Tyr Asn Trp Gly Ile Ala
35 40 45
Arg Val Ala Asp Ser Leu Asp Ala Tyr Ala Glu Gln Lys Ala Ala Gly
50 55 60
Ile Asp Glu Pro Asp Val Lys Phe Pro Gly His Phe Asp Leu Cys Lys
65 70 75 80
Met Trp Thr Ala Trp Lys Asn Thr Ala Glu Trp Thr Asp Arg His Thr
85 90 95
Gly Gln Thr Thr Thr Gly Val Pro Trp Val Ala Ser Asn Phe Val Gly
100 105 110
Thr Tyr Gln Ala Ala Leu Arg Asp Ala Ala Gly Ala Trp Gln Arg Phe
115 120 125
Phe Arg Ala Arg Lys Thr Gly Ala Arg Ala Gly Arg Pro Arg Phe Lys
130 135 140
Lys Arg Gly Arg Ala Arg Asp Ser Phe Gln Leu His Gly Asp Gly Leu
145 150 155 160
Arg Ile Val Asp Ala Lys His Val Asn Leu Pro Lys Ile Gly Thr Val
165 170 175
Lys Thr Phe Glu Ala Thr Arg Lys Leu Ala Arg Arg Leu Ala Lys Gly
180 185 190
Ser Val Pro Cys Pro Thr Cys Arg Ala Thr Gly Lys Ile Thr Asp Ser
195 200 205
Ala Ser Gly Lys Val Lys Lys Cys Ser Asp Cys Lys Ala Ala Gly Ser
210 215 220
Arg Pro Ala Ala Arg Ile Val Arg Gly Thr Val Ala Arg Asp Ser Ala
225 230 235 240
Gly Arg Trp Tyr Leu Ala Leu Thr Val Glu Leu Val Arg Glu Val Arg
245 250 255
Thr Ala Pro Thr Pro Arg Gln Leu Ala Gly Gly Pro Val Gly Val Asp
260 265 270
Phe Gly Val Arg Gln Val Ala Thr Leu Ser Thr Gly Gln Leu Val Asp
275 280 285
Asn Pro Arg His Leu Glu Ser His Leu Arg Arg Val Lys Thr Ala Gln
290 295 300
Gln Ala Leu Ser Arg Cys Pro Pro Gly Ser Arg Arg Arg Ala Lys Ala
305 310 315 320
Gln Gln Arg Leu Gly Arg Leu His Ala Arg Val Arg His Leu Arg Glu
325 330 335
Asn Ser Leu Gln Gln Ala Thr Ser Ala Leu Ile His Gln His Ser Val
340 345 350
Ile Ala Val Glu Gly Trp Asp Val Gln Gln Thr Ala Gln His Ala Ser
355 360 365
Pro Lys Asn Leu Pro Lys Gln Ile Arg Arg Asn Arg Asn Arg Ala Leu
370 375 380
Leu Asp Thr Gly Ile Gly Ala Ala Arg Trp Gln Leu Gln Ser Lys Gly
385 390 395 400
Ala Trp Tyr Gly Thr Thr Val Val Val Thr Asp Arg His Ala Pro Thr
405 410 415
Gly Arg Gln Cys Ser Ala Cys Gly Thr Val Lys Ala Thr Pro Ile Pro
420 425 430
Pro Thr Gln Asp Glu Tyr Arg Cys Pro Ala Cys Gly Thr Ser Leu Asp
435 440 445
Arg Arg Thr Asn Thr Ala Arg Val Leu Ala Ala Val Ala Ala Gln His
450 455 460
His Asp Ala Pro Ser Gly Gly Glu Ser Lys Asn Ala Arg Gly Glu Asn
465 470 475 480
Thr Arg Pro Thr Ala Pro Arg Arg Asn Gly Gln Phe Ser Ala Lys Arg
485 490 495
Glu Pro Arg Ser Arg Pro Pro Gly Arg Gly Gln Thr Gly Thr Pro Gly
500 505 510
Thr
<210> 42
<211> 522
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 42
Met Ser Glu Gly Val Ser Gly Met Ala Glu Val Leu Arg Ala Phe Lys
1 5 10 15
Phe Thr Leu Asp Pro Thr Arg Ala Gln Val Gly Ala Leu Gln Gln His
20 25 30
Ala Gly Ala Ala Arg Trp Ala Phe Asn Trp Ala Leu Gly Glu Lys Val
35 40 45
Ala Ala His Arg Glu Trp Arg Arg Gln Val Gly Ala Leu Leu Ala Glu
50 55 60
Gly Val Ala Glu Glu Gln Ala Arg Lys Gln Val Arg Val Pro Val Pro
65 70 75 80
Thr Lys Pro Thr Ile Gln Lys Arg Leu Asn Ser Phe Lys Gly Asp Ser
85 90 95
Arg Val Gln Asp Leu Pro Asp Gly Val Leu Gly Pro Arg Arg Pro Cys
100 105 110
Pro Trp Trp Trp Glu Val Lys Thr Tyr Cys Phe Gln Ala Ala Met Ala
115 120 125
Asp Ala Asp Thr Ala Trp Lys Asn Trp Leu Ser Ser Leu Thr Gly Ala
130 135 140
Arg Ala Gly Gln Arg Val Gly Tyr Pro Arg Phe Lys Lys Lys Gly Arg
145 150 155 160
Ala Arg Asp Ser Phe Arg Leu His His Asp Val Lys Lys Pro Gly Ile
165 170 175
Arg Leu Ala Gly Tyr Arg Arg Leu Arg Leu Pro Thr Ile Gly Glu Val
180 185 190
Arg Leu His Asp Phe Gly Lys Arg Leu Ala Arg Leu Ile Asp Arg Gly
195 200 205
Arg Ala Val Val Gln Ser Val Thr Val Ala Arg Cys Gly His Arg Trp
210 215 220
Tyr Ala Ser Val Leu Cys Lys Val Asp Gln Ser Val Pro Gln Arg Ser
225 230 235 240
Thr Arg Ala Gln Arg Arg Arg Gly Arg Val Gly Val Asp Leu Gly Val
245 250 255
Lys His Leu Ala Ala Leu Ser Gln Pro Leu His Pro Tyr Asp Arg Ala
260 265 270
Ser Leu Tyr Val Glu Asn Pro Arg His Leu Arg Arg Ala Ala Gln Arg
275 280 285
Leu Ala Lys Ala Gln Arg Ala Leu Ala Arg Thr Gln Lys Gly Ser Lys
290 295 300
Arg Arg Ala Lys Ala Val Arg Arg Val Gly Arg Leu His His Glu Val
305 310 315 320
Ala Val Arg Arg Glu Ser Thr Leu His Gln Leu Thr Lys Arg Leu Ala
325 330 335
Thr Gly Phe Ala Glu Val Ala Val Glu Asp Leu His Val Ala Gly Met
340 345 350
Thr Arg Ser Ala Lys Gly Thr Ile Asp Ala Pro Ser Arg Asn Val Arg
355 360 365
Ala Lys Ala Gly Leu Asn Arg Ser Ile Leu Asp Thr Ala Pro Gly Glu
370 375 380
Leu Arg Arg Gln Leu Thr Tyr Lys Thr Cys Trp Tyr Gly Ser Arg Leu
385 390 395 400
Ala Val Leu Asp Arg Trp Trp Pro Ser Ser Lys Thr Cys Ser Ala Cys
405 410 415
Gly Arg Gln Asn Pro Arg Leu Thr Leu Ala Asp Arg Thr Phe His Cys
420 425 430
Thr Gly Cys Gly Leu Arg Ile Asp Arg Asp Leu Asn Ala Ser Arg Asn
435 440 445
Ile Ala Thr His Ala Ala Leu Ala Asp Thr Ala Pro Pro Val Ala Pro
450 455 460
Asp Arg Gly Glu Thr Gln Asn Ala Arg Arg Ala Gly Thr Arg Pro Thr
465 470 475 480
Gly Pro Arg Ala Gly Arg His Pro Ala Thr Lys Arg Glu Asp Thr Ala
485 490 495
Pro Ala Val Pro Pro Gln Arg Ser Asn Pro Leu Ala Leu Pro Pro Pro
500 505 510
Pro Thr Gly Tyr Glu Gln Val Thr Leu Phe
515 520
<210> 43
<211> 519
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 43
Met Ala Glu Thr Glu Val Leu Arg Ala Phe Arg Phe Ala Leu Asp Ala
1 5 10 15
Thr Ala Ala Gln Glu Glu Gly Phe Leu Arg His Ala Gly Ala Ser Arg
20 25 30
Trp Ala Phe Asn His Ala Leu Gly Met Lys Val Ala Ala His Arg Gln
35 40 45
Trp Gln Arg Glu Val Lys Ala Leu Val Glu Gly Gly Met Pro Glu Ala
50 55 60
Gln Ala Arg Lys Thr Val Lys Val Pro Val Pro Thr Arg Pro Thr Ile
65 70 75 80
Arg Lys His Leu Asn Arg Ile Lys Gly Asp Ser Arg Ser Pro Asp Leu
85 90 95
Pro Glu Gly Ala Gln Gly Pro Gln Arg Pro Cys Pro Trp Phe His Glu
100 105 110
Val Ser Thr Tyr Ala Phe Gln Ser Ala Phe Glu Asp Ala Asp Arg Ala
115 120 125
Trp Gly Asn Trp Gln Ala Ser Leu Ser Gly Arg Arg Ala Gly Arg Lys
130 135 140
Val Gly Tyr Pro Arg Phe Lys Lys Lys Gly Arg Thr Lys Asp Ser Phe
145 150 155 160
Arg Ile Cys His Asp Ala Lys Lys Pro Thr Ile Arg Pro Asp Gly Tyr
165 170 175
Arg Arg Leu Arg Ile Pro Ala Leu Gly Ser Val Arg Leu His Asp Thr
180 185 190
Ala Lys Pro Leu Ala Arg Leu Met Asp Arg Gly Ala Ile Val Lys Ser
195 200 205
Val Met Val Ser Arg Ser Gly Ala Arg Trp Tyr Ala Ser Val Leu Val
210 215 220
Ser Val Leu Gln Asp Ile Pro Glu Arg Pro Thr Arg Arg Gln Arg Gln
225 230 235 240
Ala Gly Thr Val Gly Val Asp Phe Gly Val Lys Thr Leu Ala Ala Leu
245 250 255
Ser Ala Pro Val Thr Leu Pro Asn Leu Gly Thr Leu Thr Met Val Pro
260 265 270
Asn Pro Arg His Leu Ala Ser Asp Thr Arg Arg Leu Thr Lys Ala Gln
275 280 285
Arg Ala Leu Ser Arg Thr Thr Lys Gly Ser Ala Arg Arg Arg Lys Ala
290 295 300
Ala Arg Arg Val Gly Val Val His His Arg Ile Ala Glu Arg Arg Ala
305 310 315 320
Thr Tyr Leu His Thr Leu Thr Ser Gln Leu Ala Thr Gly Tyr Ala Val
325 330 335
Val Ala Val Glu His Leu Asn Val Ala Gly Met Thr Ser Ser Ala Arg
340 345 350
Gly Thr Val Glu Glu Pro Gly Ser Lys Val Arg Gln Lys Ser Gly Leu
355 360 365
Asn Arg Ser Ile Leu Asp Ala Ser Pro Ala Glu Met Arg Arg Gln Leu
370 375 380
Asp Tyr Lys Thr Arg Trp Asn Gly Ser Gln Leu Ala Val Cys Glu Arg
385 390 395 400
Trp Phe Pro Ser Ser Arg Thr Cys Ser Ala Cys Gly Trp Gln Lys Pro
405 410 415
Arg Leu Thr Leu Ala Glu Arg Val Phe Ala Cys Gly Gln Cys Gly Leu
420 425 430
Val Ile Asp Arg Asp Leu Asn Ala Ala Arg Asn Ile Ala Ala His Ala
435 440 445
Val Leu Val Pro His Gly Thr Ala Ala Pro Gly Ser Gly Glu Ala Ser
450 455 460
Asn Ala Arg Gly Ala Ala Thr Arg Pro Ala Thr Pro Arg Gly Gly Arg
465 470 475 480
Gln Ala Ala Leu Lys Arg Glu Asp Thr Gly Pro Pro Arg Pro Val Pro
485 490 495
Pro Gln Arg Ser Asp Pro Leu Thr Leu Phe Thr Leu Asp Val Pro Asp
500 505 510
Gln Gln Thr Ala Lys Arg Pro
515
<210> 44
<211> 518
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 44
Met Gln Thr Glu Val Leu Arg Ala Phe Arg Phe Thr Leu Asp Pro Thr
1 5 10 15
Pro Ala Gln Gln Glu Asp Leu Leu Arg His Ala Gly Ala Ala Arg Trp
20 25 30
Ala Phe Asn His Ala Leu Gly Val Lys Ile Ala Ala His Gln Gln Trp
35 40 45
Arg Thr Lys Val Gln Ala Leu Val Asp Ser Gly Val Ala Glu Ser Ile
50 55 60
Ala Arg Lys Gln Val Arg Val Pro Val Pro Met Lys Pro Glu Ile Gln
65 70 75 80
Lys His Leu Asn Arg Ile Lys Gly Asp Ser Gln Lys Gln Pro Trp Pro
85 90 95
Ala Gly Ser Ile Gly Pro Ala Arg Pro Cys Pro Trp Trp Arg Glu Val
100 105 110
Ser Thr Tyr Ala Phe Gln Ser Ala Phe Ile Asp Ala Asp Gln Ala Trp
115 120 125
Lys Asn Trp Leu Asp Ser Leu Ala Gly Arg Arg Ala Gly Arg Lys Val
130 135 140
Gly Tyr Pro Arg Phe Lys Lys Lys Ala Arg Ser Lys Asp Ser Phe Arg
145 150 155 160
Leu His His Thr Val Thr Gln Pro Thr Ile Arg Leu Asp Gly Tyr Arg
165 170 175
Arg Leu Thr Leu Pro Arg Leu Gly Thr Ile Arg Val His Asp Ser Gly
180 185 190
Lys Arg Leu Ala Arg Leu Ile Thr Arg Gly His Ala Val Val Gln Ser
195 200 205
Val Thr Val Ser Arg Ser Ala Asn Arg Trp Tyr Ala Ala Val Leu Val
210 215 220
Lys Val Arg Gln Asn Leu Pro Asp Arg Pro Thr Arg Arg Gln Gln Ser
225 230 235 240
Asn Gly Thr Val Gly Val Asp Leu Gly Val Lys Thr Leu Ala Ala Leu
245 250 255
Thr Gln Pro Val Thr Leu Pro Gly Ala Thr Asp Thr Leu Leu Val Pro
260 265 270
Asn Pro Arg His Leu Ala Ala Asp Thr Arg Arg Leu Thr Arg Ala Gln
275 280 285
Arg Ala Leu Ser Arg Thr Thr Lys Gly Ser Thr Arg Arg Arg Lys Val
290 295 300
Ala Arg Arg Val Ala Gln Leu His His Arg Ile Ala Glu Arg Arg Ala
305 310 315 320
Thr Tyr Leu His Thr Leu Thr Lys Gln Leu Thr Thr Arg Tyr Ala Ala
325 330 335
Val Ala Val Glu Asp Leu Asn Val Ser Gly Met Ser Ala Ser Ala Arg
340 345 350
Gly Thr Val Glu Glu Pro Gly Arg Lys Val Arg Gln Lys Ala Gly Leu
355 360 365
Asn Arg Ala Ile Leu Asp Met Ala Pro Ala Glu Ile Arg Arg Gln Leu
370 375 380
Gly Tyr Lys Thr Arg Trp Asn Gly Ser Arg Leu Ala Val Cys Asp Arg
385 390 395 400
Tyr Tyr Pro Ser Ser Lys Thr Cys Ser Ala Cys Gly Trp Gln Asn Pro
405 410 415
Arg Leu Thr Leu Ala Asp Arg Thr Phe Ile Cys Gly Gln Cys Gly Leu
420 425 430
Thr Ile Asp Arg Asp Leu Asn Ala Ala Arg Asn Ile Ala Ala His Ala
435 440 445
Val Pro Thr Pro Leu His Ser Thr Val Ala Pro Gly Thr Glu Glu Thr
450 455 460
Gln Asn Ala Arg Arg Ala Ala Thr Arg Pro Thr Thr Pro Arg Gly Gly
465 470 475 480
Arg Gln Thr Ala Lys Lys Arg Glu Asp Thr Gly Arg Gln Pro Pro Val
485 490 495
Pro Pro Gln Arg Ser Asp Pro Leu Thr Leu Gln Pro Pro Gln His Gln
500 505 510
Glu Val Ala Lys Gly Pro
515
<210> 45
<211> 506
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 45
Met Ala Ser Thr Lys Thr Val Val Leu Arg Ala Phe Lys Phe Thr Leu
1 5 10 15
Ala Pro Thr Ala Thr Gln Asp Gln Gln Leu Leu Arg Trp Cys Gly Asn
20 25 30
Ala Arg Leu Ala Phe Asn Tyr Ala Leu Ala Ser Lys Arg Ala Ala His
35 40 45
Thr Glu Trp Arg Ala Gln Val Asp Ala Leu Val Thr Ser Gly Val Lys
50 55 60
Glu Pro Val Ala Arg Lys Arg Val Thr Gly Pro Lys Thr Pro Thr Lys
65 70 75 80
Pro Ala Val Tyr Lys Ala Phe Ile Ala Glu Arg Gly Asp Thr Arg Glu
85 90 95
Gly Leu Asp Gly Val Cys Pro Trp Ala His Glu Ile Asn Thr His Val
100 105 110
Phe Gln Ser Ala Phe Ile Asp Ala Asp Arg Ala Trp Lys Asn Trp Leu
115 120 125
Asp Ser Phe Lys Gly Thr Arg Lys Gly Arg Arg Val Gly Tyr Pro Arg
130 135 140
Phe Lys Lys Arg Gly Arg Ala Arg Asp Ala Phe Arg Leu His His Thr
145 150 155 160
Val Thr Lys Pro Thr Ile Arg Phe Ser Thr His Arg Arg Leu Arg Leu
165 170 175
Pro Thr Phe Gly Glu Val Arg Leu His Asp Ser Ala Arg Thr Leu Val
180 185 190
Arg Gln Ile Asp Arg Gly Thr Ala Val Val Gln Ser Val Thr Val Ser
195 200 205
Arg Ala Gly His Arg Trp Tyr Ala Ser Val Leu Cys Lys Val Glu Met
210 215 220
Asp Leu Pro Ser Gly Pro Thr Arg Ser Gln Gln Ala Ala Gly Thr Val
225 230 235 240
Gly Ile Asp Phe Gly Val Lys Ala Leu Ala Ala Leu Ser Lys Pro Leu
245 250 255
Val Pro Asp Arg Pro Glu Ser Thr Leu Leu Pro Asn Pro Arg His Leu
260 265 270
Ala Lys Ala Ala His Arg Leu Lys Arg Ala Gln Gln Thr Leu Ser Arg
275 280 285
Arg Gln Lys Gly Ser Ala Arg Arg Glu Lys Ala Arg Arg Arg Val Ala
290 295 300
Arg Leu His His Glu Val Ala Val Arg Arg Gln Ser Ala Leu His Gln
305 310 315 320
Ile Thr Lys Arg Leu Thr Thr Arg Phe Ala Thr Ile Ala Val Glu Asp
325 330 335
Leu His Val Ser Gly Met Thr Arg Ser Ala Arg Gly Thr Met Asp Lys
340 345 350
Pro Gly Arg Lys Val Arg Gln Lys Ala Gly Leu Asn Arg Ala Ile Leu
355 360 365
Asp Ala Ser Met Ala Glu Ala Arg Arg Gln Ile Thr Tyr Lys Thr Ser
370 375 380
Trp Tyr Gly Ser Arg Leu Ala Val Leu Asp Arg Trp Trp Pro Ser Ser
385 390 395 400
Lys Thr Cys Ser Ala Cys Gly Trp Gln Asn Pro Ser Leu Thr Leu Ala
405 410 415
Asp Arg Val Phe Glu Cys Ala Gln Cys Gly Leu Thr Leu Asp Arg Asp
420 425 430
Leu Asn Ala Ala Arg Asn Ile Glu Gln His Ala Val Gln Val Ala Ser
435 440 445
Gly Thr Gly Glu Thr Gln Asn Ala Arg Gly Glu Pro Val Arg Leu Pro
450 455 460
Arg Pro Arg Ala Glu Lys Gln Gly Ser Thr Lys Arg Glu Asp Thr Gly
465 470 475 480
Pro Pro Gly Pro Val Pro Pro Arg Arg Ser Asp Pro Pro Thr Pro Pro
485 490 495
Asn Pro Arg Gln Gly Gln Ala Lys Leu Phe
500 505
<210> 46
<211> 477
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 46
Met Ser Val Ala His Val Ser Leu Leu Asn Met Thr Thr Thr Glu Asn
1 5 10 15
Thr Thr Thr Ile Val Val Arg Glu Pro Leu Asp Pro Thr Pro Glu Gln
20 25 30
Val Leu Val Leu Lys Arg Tyr Ala Asn Ala Ser Arg Ala Ser Phe Asn
35 40 45
Phe Ala Tyr Gly Leu Lys His Glu Ala Gln Gln Arg Trp Val Arg Gly
50 55 60
Arg Asp Arg Leu Leu Ser Lys Gly Leu Gly Arg Glu Glu Ala Asn Arg
65 70 75 80
Arg Ala Pro Lys Val Leu Val Pro Arg Gly Ser Asp Val Gln Arg Ile
85 90 95
Phe Leu Ala Val Arg Glu Gln Pro Leu Ala Gly Pro Leu Arg Glu Gly
100 105 110
Glu Ser Glu His Arg Arg Met Phe Arg Trp Trp Ala Gly Val Asn Ala
115 120 125
Ile Val Cys Gln Gln Ala Phe Arg Asp Ala Asp Thr Ala Phe Ala Asn
130 135 140
Trp Arg Ser Ser Gly Arg Arg Ala Gly Glu Gly Val Gly Tyr Pro Arg
145 150 155 160
Pro Lys Arg Val Gly Arg Cys Arg Asp Ser Phe Arg Met Thr Ser Val
165 170 175
Arg Leu Val Gly Thr Asp Leu Arg His Val Arg Ile Gly Gly Glu Lys
180 185 190
Asp Pro Ala Gly Gln Arg Ala Leu Ile Val Arg Leu His Arg Pro Gly
195 200 205
Arg Arg Leu Ala Arg Ala Met Ala Arg Gly Gly Val Val Lys Met Val
210 215 220
Thr Val Ala Arg Glu Gly Ser Gln Trp Trp Ala Ser Phe Asn Val Arg
225 230 235 240
Ile Val Leu Pro Pro Pro Ala Gln Pro Ser Arg Arg Gln Arg Glu Ala
245 250 255
Gly Thr Val Gly Val Asp Leu Gly Val Ala Val Phe Ala Ala Thr Ser
260 265 270
Glu Pro Val Leu Thr Ala Ala Gly Lys Glu Gln Leu Phe Asp Asn Pro
275 280 285
Arg His Leu Asp Asn Ala Arg Arg Gln Leu His Lys Trp Gln Arg Arg
290 295 300
Met Ala Arg Arg His Val Lys Gly Leu Pro Val His Arg Gln Ser Ala
305 310 315 320
Gly Trp Arg Glu Ala Arg Asp Gln Val Ala His Leu Met Gly Leu Val
325 330 335
Ala Gln Arg Arg Ala Ser Thr Gln His Leu Leu Thr Lys Gln Leu Val
340 345 350
Thr Gln Phe Glu His Val Ala Leu Glu Asp Leu Arg Val Lys Asn Met
355 360 365
Thr Arg Thr Ala Arg Gly Thr Val Glu Ala Pro Gly Arg Asn Val Ala
370 375 380
Ala Lys Ala Gly Leu Asn Arg Ala Ile Leu Asp Val Gly Phe Gly Glu
385 390 395 400
Ile Arg Arg Gln Ile Glu Tyr Lys Ala Lys Trp His Gly Val Thr Val
405 410 415
Thr Ala Val Asn Pro Ala Tyr Thr Ser Gln Thr Cys His Arg Cys Gly
420 425 430
His Val Asp Arg Lys Ser Arg Arg Thr Arg Ser Val Phe Glu Cys Thr
435 440 445
Arg Cys Gly His Val Thr His Ala Asp Ile Gly Ala Ala His Asn Ile
450 455 460
Lys His Arg Ala Leu Ala Pro Asp Ala Asn Asp Ala Arg
465 470 475
<210> 47
<211> 509
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 47
Met His Thr Glu Val Leu Arg Ala Tyr Arg Phe Ala Leu Asp Pro Thr
1 5 10 15
Arg Gly Gln Leu Glu Asp Leu Ala Arg His Ala Gly Ala Ala Arg Trp
20 25 30
Ala Phe Asn His Ala Leu Ala Ala Lys Val Ala Ala His Lys Glu Trp
35 40 45
Arg Ala Lys Val Ala Ala Leu Val Glu Ala Gly Thr Ala Glu Ala Glu
50 55 60
Ala Arg Lys Gln Val Arg Val Pro Ile Pro Thr Lys Pro Gly Ile Gln
65 70 75 80
Lys Ala Leu Asn Ala Ala Lys Gly Asp Ser Arg Thr Gly Thr Asp Gly
85 90 95
Leu Cys Pro Trp Trp His Glu Val Asn Thr Tyr Cys Phe Gln Ser Ala
100 105 110
Phe Ala Asp Ala Asp Arg Ala Trp Lys Asn Trp Leu Asp Ser Leu Lys
115 120 125
Gly Val Arg Ala Gly Arg Lys Val Gly Tyr Pro Arg Phe Lys Glu Lys
130 135 140
Gly Arg Ala Arg Asp Ser Phe Arg Leu His His Asp Val Lys Lys Pro
145 150 155 160
Gly Ile Arg Met Val Gly Tyr Arg Arg Leu Arg Leu Pro Lys Val Gly
165 170 175
Glu Val Arg Leu His Gly Ser Gly Arg Glu Leu Ala Arg Ala Val Asp
180 185 190
Arg Gly Arg Ala Val Val Gln Ser Val Thr Val Ala Arg Asp Gly His
195 200 205
Arg Trp Tyr Ala Ser Val Leu Cys Ala Val Ala Met Asp Val Arg Glu
210 215 220
Lys Pro Thr Gln Arg Gln Thr Met Arg Gly Thr Val Gly Val Asp Leu
225 230 235 240
Gly Val Lys Tyr Leu Ala Ala Leu Ser Gln Pro Leu Thr Glu Gly Asp
245 250 255
Glu Ala Ser Lys Phe Val Ala Asn Pro Arg His Leu Lys Ala Ala Glu
260 265 270
Lys Arg Leu Val Lys Ala Gln Arg Ala Phe Ser Arg Thr Gln Lys Gly
275 280 285
Ser Gly Arg Arg Asp Lys Ala Arg Arg Arg Val Ala Arg Leu His His
290 295 300
Gln Val Ala Arg Gln Arg Ala Gly Ala Met His Gln Phe Thr Lys Arg
305 310 315 320
Leu Ala Thr Gly Phe Ala Val Val Ala Val Glu Asp Leu Asn Val Ala
325 330 335
Gly Met Thr Arg Ser Ser Arg Gly Thr Val Glu Ala Pro Gly Arg Arg
340 345 350
Val Arg Gln Lys Ala Gly Leu Asn Arg Ala Ile Leu Asp Val Ala Pro
355 360 365
Gly Glu Leu Arg Arg Gln Leu Ala Tyr Lys Thr Ser Trp Tyr Gly Ser
370 375 380
Lys Leu Ala Val Val Asp Arg Trp Phe Pro Ser Ser Lys Thr Cys Ser
385 390 395 400
Asn Cys Gly Trp Gln Asn Pro Ser Leu Thr Leu Ser Asp Arg Thr Phe
405 410 415
His Cys Ser Asn Cys Glu Ser Ala Met Asp Arg Asp Trp Asn Ala Ala
420 425 430
Arg Asn Ile Ala Arg His Ala Val Leu Gly Asp Ser Gln Val Ala Cys
435 440 445
Asp Arg Arg Glu Thr Glu Asn Ala Arg Gly Ala Leu Val Ser Pro Gly
450 455 460
Ala Ser Arg Gly Gly Arg Arg Arg Ala Val Lys Arg Glu Asp Thr Gly
465 470 475 480
Pro Pro Pro Val Pro Pro Gln Arg Ser Asp Pro Leu Ala Leu Pro Thr
485 490 495
Pro Thr Arg Lys Val Thr Gly Ile Gln Ala Ser Leu Phe
500 505
<210> 48
<211> 468
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 48
Met Thr Thr Thr Ser Thr Thr Glu Thr Leu Arg Ala Tyr Arg Tyr Ala
1 5 10 15
Leu Asp Pro Thr Pro Ala Gln Ile Glu Ile Leu Gln Arg Tyr Ala Thr
20 25 30
Ala Ala Arg Cys Gly Tyr Asn Phe Ala Leu Gly Tyr Met Val Ala Val
35 40 45
His Gln Lys Trp Ala Arg Gly Arg Asp Ala Leu Ile Ala Ala Gly Met
50 55 60
Asp Lys Ala Val Ala Asn Lys Ala Ala Pro Lys Val Lys Val Pro Asn
65 70 75 80
Ala Phe Arg Ala Gln Ala Phe Phe Arg Glu Thr Lys Gly His Pro Phe
85 90 95
Thr Gly Pro Leu Pro Glu Gly Ala Glu Arg Thr Thr Pro Tyr Pro Trp
100 105 110
Trp Glu Gly Val Ser Asn Arg Ala Tyr Tyr Thr Ala Met Glu Asp Ala
115 120 125
Ala Thr Ala Trp Lys Asn Trp Met Asp Ser Ala Ser Gly Arg Arg Ala
130 135 140
Gly Gly Pro Val Gly Tyr Pro Arg Phe Lys Arg Arg Gly Arg Ala Arg
145 150 155 160
Glu Arg Phe Arg Leu Val His Asn Val Lys Lys Pro Glu Ile Arg Phe
165 170 175
Glu Thr Ser Arg Arg Leu Arg Ile Pro Gly Gly Gly Gly Gln Ser Ala
180 185 190
Phe Thr Val Arg Leu His Gln Asp Ala Arg Asp Leu Leu Arg Leu Ile
195 200 205
Ala Asp Gly Arg Ala Val Val Asn Ser Leu Thr Val Ser Arg Asp Gly
210 215 220
His Arg Trp His Ala Ser Val Leu Cys Arg Val Glu Gln His Ile Pro
225 230 235 240
Ser Gly Pro Ser His Arg Gln Ala Ala Ala Gly Arg Ile Gly Ala Asp
245 250 255
Leu Gly Val Lys Ala Leu Ala Ala Leu Ser Asp Pro Leu Thr Leu Thr
260 265 270
Pro Ala Ala Gly Pro Thr Val Leu Ile Pro Asn Pro Arg His Leu Ala
275 280 285
Ala Thr Glu Arg Lys Leu Ala Arg Ala Gln Arg Val Met Ser Arg Arg
290 295 300
Phe Val Arg Gly Ala Ala Gln Gln Ser Lys Gly Tyr Ser Glu Ala Arg
305 310 315 320
Ala Arg Val Ala Lys Leu His Ala Gln Leu Ala Ala Arg Arg Thr Ser
325 330 335
Ala Leu His Leu Ile Ser Lys Arg Leu Val Gln Gln Tyr Ala Glu Ile
340 345 350
Ala Leu Glu Ser Leu Asn Thr Lys Gly Met Thr Ser Ser Ala Lys Gly
355 360 365
Thr Leu Gln Ser Pro Gly Arg Asn Val Arg Gln Lys Ala Gly Leu Asn
370 375 380
Arg Ala Ile Leu Asp Ala Ser Phe Gly Glu Leu Asn Arg Gln Ile Ala
385 390 395 400
Tyr Lys Ala Ala Trp His Gly Ala Thr Leu Ala Arg Val Pro Thr Phe
405 410 415
Phe Pro Ser Ser Lys Thr Cys Ser Ala Cys Gly Trp Ile Asn Thr Glu
420 425 430
Leu Thr Leu Ala Asp Arg Glu Phe Ala Cys Arg Ser Cys Gly Ile Val
435 440 445
Leu Asp Arg Asp Ala Asn Ala Ala Arg Asn Ile Lys Asn His Ala Ile
450 455 460
Pro Val Gln Pro
465
<210> 49
<211> 462
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 49
Met Thr Asp Thr Ile Ile Lys Ala Phe Arg Tyr Ala Leu Asp Pro Thr
1 5 10 15
Glu Ala Gln Ile Glu Ile Leu His Arg Tyr Ala Thr Ala Ser Arg Cys
20 25 30
Gly Phe Asn Phe Ala Leu Gly Met Lys Thr Thr Val Tyr Asp Arg Trp
35 40 45
Arg Arg Gly Arg Asp Ala Leu Val Ala Ala Gly Met Asp Lys Ala Glu
50 55 60
Ala Asn Lys Lys Ala Pro Lys Val Arg Val Pro Asn Arg Asn Arg Thr
65 70 75 80
Gln Ala Tyr Trp Arg Glu Thr Arg Gly Gln Gly Phe Ile Gly Pro Leu
85 90 95
Arg Glu Gly Gln Glu Pro Arg Ala Pro Phe Ala Trp Trp Glu Gly Val
100 105 110
Asn Asn Arg Ala Tyr Tyr Thr Ala Phe Glu Asp Ala Asp Thr Ala Trp
115 120 125
Lys Asn Trp Leu Asp Ser Leu Ala Gly Arg Arg Pro Ala Met Gly Phe
130 135 140
Pro Lys Phe Lys Arg Arg Gly Ala Ser Arg Glu Ser Phe Arg Ile Val
145 150 155 160
His Ser Leu Lys Asn Pro Asp Ile Arg Phe Asp Gly Pro Arg Arg Leu
165 170 175
Arg Ile Pro Gly Gly Gly Gly Gln Pro Ala Phe Thr Val Arg Leu Leu
180 185 190
Gln Ser Pro Arg Ala Leu Thr Ser Leu Ile Ala Ser Gly Gln Ala Val
195 200 205
Ile Thr Ser Val Thr Val Ser Arg Glu Gly His Arg Trp His Ala Ser
210 215 220
Val Leu Ala Arg Ile Glu Gln Asp Leu Pro Thr Arg Pro Thr Arg Arg
225 230 235 240
Gln Gln Ala Ala Gly Arg Ile Gly Ile Asp Leu Gly Val Lys Thr Ala
245 250 255
Leu Thr Leu Ser Asp Pro Leu Thr Leu His Arg Gly Gln Ala Pro Val
260 265 270
Leu Ala Ile Asp Asn Pro Arg Leu Leu Glu Asn Thr Ala Arg Lys Leu
275 280 285
Ala Arg Ala Gln Arg Val Met Ala Arg Arg His Val Lys Gly Ala Ala
290 295 300
Gln Gln Ser Gln Gly Tyr Leu Glu Ala Lys Ala Arg Val Ala Lys Leu
305 310 315 320
His Ala Leu Leu Ala Ala Arg Arg Ser Thr Ala Gln His Leu Val Thr
325 330 335
Lys Arg Leu Val Glu Gln Tyr Ala Glu Ile Ala Leu Glu Thr Leu Asn
340 345 350
Thr Lys Gly Met Thr Arg Ser Ala Lys Gly Thr Val Asp Lys Pro Gly
355 360 365
Arg Asn Val Arg Gln Lys Ser Gly Leu Asn Arg Ala Leu Leu Asp Val
370 375 380
Gly Phe Ala Glu Ile Asn Arg Gln Ile Glu Tyr Lys Ala Gly Trp Arg
385 390 395 400
Ala Val Thr Ile Ala Arg Val Pro Gly Leu Phe Pro Ser Ser Lys Ser
405 410 415
Cys His Arg Cys Gly Trp Ile His Thr Asn Leu Thr Leu Ala Asp Arg
420 425 430
Glu Phe Arg Cys Asp Ala Cys Gly Met Ile Ile Asp Arg Asp Val Asn
435 440 445
Ala Ala Gln Asn Ile Lys Asn His Ala Thr Pro Asp Arg Pro
450 455 460
<210> 50
<211> 530
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 50
Met Ala Leu Gln Arg Ile Gln Gln Ala Phe Lys Tyr Ala Leu Asp Pro
1 5 10 15
Thr Pro Ala Gln Ala Arg Met Leu Thr Ser His Ala Gly Ala Ala Arg
20 25 30
Tyr Ala Phe Asn Trp Gly Leu Ala Thr Phe Ala Thr Ala Leu Asp Ala
35 40 45
Tyr Ser Ala Glu Lys Thr Ala Gly Val Lys Lys Pro Val Thr Lys Leu
50 55 60
Pro Gly His Phe Asp Leu Cys Lys Leu Trp Thr Ala His Lys Asp Asn
65 70 75 80
Pro Thr Ser Asp Leu Gly Trp Val Gly Gln Asn Phe Ser Gly Thr Tyr
85 90 95
Gln Ala Ala Leu Arg Asp Ala His Ala Ala Trp Lys Ala Phe Leu Asp
100 105 110
Ser Lys Asn Gly Arg Arg Arg Gly Arg Gln Val Gly Arg Pro Arg Phe
115 120 125
Lys Ser Arg His Arg Thr Thr Ala Ala Phe Gln Thr His Gly Thr Gly
130 135 140
Leu Arg Val Ala Asp His Arg His Ile Asn Leu Pro Lys Ile Gly Pro
145 150 155 160
Val Arg Ser Tyr Glu Lys Thr Lys Lys Leu Arg Arg Leu Leu Thr Arg
165 170 175
Pro Asp Val Thr Cys Thr Thr Cys Asp Gly Thr Lys Glu Ala Pro Ala
180 185 190
Pro Ala Pro Ala Thr Lys Ser Cys Gly Asp Cys Lys Gly Thr Gly His
195 200 205
Ala Pro His Thr Arg Ile Val Arg Gly Asn Ile Thr Arg Thr Pro Ser
210 215 220
Gly Arg Trp His Ile Ser Leu Thr Val Glu Thr His Arg Asp Ile Arg
225 230 235 240
Thr Arg Pro Ser Gln Arg Gln Arg Glu Gly Gly Ile Val Gly Val Asp
245 250 255
Trp Gly Val Arg Asp Leu Ala Thr Leu Ser Thr Gly Glu Val Ile Gly
260 265 270
Asn Pro Arg His Leu Glu Arg Asn Leu Ala Arg Leu Arg Arg Ala Gln
275 280 285
Gln Asp Leu Ala Arg Lys Ala Asp Gly Ser Ala Gly Arg Glu Ala Ala
290 295 300
Arg Leu Arg Val Ala Arg Leu His Gly Arg Val Ala Asn Leu Arg Arg
305 310 315 320
Asp His Leu Glu Lys Val Thr Ser Arg Leu Val His Ser His Thr Arg
325 330 335
Ile Val Val Glu Gly Trp Asp Val Gln His Ala Met Gln His Ser Gly
340 345 350
Asp Gly Ala Pro Lys Trp Val Arg Arg Asp Arg Asn Arg Ala Leu Ser
355 360 365
Asp Thr Gly Ile Gly Ala Ala Arg Trp Met Leu Gly Arg Lys Ala Ala
370 375 380
Trp Tyr Gly Ser Ala Ile Val Glu Thr Gly Pro His Glu Pro Thr Gly
385 390 395 400
Arg Thr Cys Ser Ala Cys Ala Thr Ala Arg Thr Lys Pro Val Ala Pro
405 410 415
Ala Asp Glu Arg Phe Thr Cys Pro Ser Cys Gly Tyr Ser Gly Asp Arg
420 425 430
Arg Val Asn Thr Ala Arg Val Leu Val Arg Leu Ala His Thr Ser Asp
435 440 445
Ala Pro Ser Ser Gly Glu Ser Leu Asn Ala Arg Gly Gly Asp Val Arg
450 455 460
Pro Ala Ala Pro Ser Thr Arg Pro Gly Gly Gln Thr Pro Thr Arg Pro
465 470 475 480
Ser Gly His Ile Ala Val His Ala Leu Gly Lys Asp Glu Glu Gly Arg
485 490 495
Asp Lys Gly Lys Asp Lys Glu Phe Arg Gly Arg Arg Ser Pro Met Lys
500 505 510
Arg Glu Ala Arg Ser Arg Pro Pro Gly Arg Gly Lys Ala Gly Thr Pro
515 520 525
Asp Pro
530
<210> 51
<211> 512
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 51
Gly Thr Asp Arg Tyr Gly Lys Pro Gln Lys Pro Asp Trp Glu Ala Ala
1 5 10 15
Arg Gln Val Phe Ile Asp Gly Gly Ile Glu Asp Glu Val Ala Asp Thr
20 25 30
Ile Leu Ser Glu Trp Gln Gln Arg Tyr Arg Arg Glu Pro Ser Asp Thr
35 40 45
Lys Pro Gly Asn Asp Pro Leu Val Asp Gly Ile Ala Pro Trp Leu Ala
50 55 60
Glu Val Pro Asn Ala Leu Val Gln Arg Ala Glu Ser Asp Cys Glu Glu
65 70 75 80
Ala Trp Lys Arg Phe Tyr Lys Met Leu Arg Glu Gly Thr Ala Thr Pro
85 90 95
Ser Lys Arg Ser Arg Pro Arg Lys Thr Ala Arg Pro Asp Gly Ser Phe
100 105 110
Asp Tyr Asp Pro Pro Gly Leu Pro Arg Tyr Lys Arg Arg Ser Pro Gly
115 120 125
Arg Gly Ser Phe Tyr Leu Thr Asn Thr Glu Val Arg Leu Val Pro Asn
130 135 140
Ala Ser Arg Arg Ile Arg Leu Gly Gly Lys Ile Gly Asp Val Arg Thr
145 150 155 160
Leu Glu Gly Arg Thr Met Arg Arg Ile Arg Arg Ser Ile Ala Lys Arg
165 170 175
Asp Gly Val Ile Gln Ser Val Thr Val Ser Arg Gly Ala Ser Arg Trp
180 185 190
Tyr Ala Ser Val Leu Val Lys Glu Thr Phe Thr Pro Pro Lys Pro Thr
195 200 205
Arg Arg Gln Leu Glu Ala Gly Arg Val Gly Val Asp Val Gly Val Lys
210 215 220
His Ala Phe Val Val Ala Gly Pro Ser Thr Thr Ala Gly Gly Leu Ile
225 230 235 240
Val Asp Arg Pro Ala Arg Cys Gly Ser Glu Lys Ala Leu Asn Asp Ala
245 250 255
Arg Lys Thr Leu Arg Glu Thr Ala Pro Arg Ser Lys Ala Arg Met Lys
260 265 270
Ala Arg Ile Thr Val Ser Arg Leu Glu Asp Arg Glu Gln Trp Ala Ile
275 280 285
Glu Gln Ala Gly Arg Ala Val Ser Arg Lys Lys Leu Arg Ser Lys Asn
290 295 300
Trp Tyr Lys Ala Val Ala Arg Leu Ala Glu Leu Lys His Arg Gln Ala
305 310 315 320
Val Arg Arg Lys Thr Phe Ile His Glu Ala Thr Lys Arg Leu Ala Thr
325 330 335
Gly Tyr Ala Glu Ile Val Ile Glu Asp Leu Gln Val Lys Gly Met Ser
340 345 350
Ala Ser Ala Lys Gly Ser Ala Glu Ala Pro Gly Arg Arg Val Arg Gln
355 360 365
Lys Ala Gly Leu Asn Arg Arg Ile Leu Ala Ser Ser Phe Gly Glu Phe
370 375 380
Arg Arg Gln Leu Glu Tyr Lys Gln Gln Trp Tyr Gly Ser His Val Leu
385 390 395 400
Thr Ala Asp Arg Trp Tyr Ala Ser Ser Lys Ile Cys Ala His Cys Gly
405 410 415
Ala Thr Lys Ala Lys Leu Pro Leu Ser Ala Arg Gln Tyr Val Cys Asp
420 425 430
Ser Cys Gly Tyr Thr Ala Asp Arg Asp Val Asn Ala Ala Arg Asn Leu
435 440 445
Ala Arg Cys Ala Lys Val Ala Ser Thr Arg Lys Gly Val Ala Ser Glu
450 455 460
Val Gly Glu Thr Val Asn Asp Arg Arg Gly Arg Tyr Gly Arg Arg Arg
465 470 475 480
Ser Thr Val Ser Ala Asp Ala Ala Ala Val Pro Ala Gly Arg Pro Ala
485 490 495
Ile Asp Ala Gly His Arg Gly Gly Ser Asp Ser Ala Thr Tyr Pro Leu
500 505 510
<210> 52
<211> 758
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 52
Met Ala Ala Met Glu Thr Gln Met Ile Gln Gln Ala Tyr Leu Phe Ala
1 5 10 15
Leu Asp Pro Thr Gln Ala Gln Ala Ala Thr Leu Ala Ser His Ala Gly
20 25 30
Ala Arg Arg Tyr Ala Phe Asn Trp Ala His Ala Met Ile Ala Ala Ala
35 40 45
Ala Asp Ala Arg Gln Ala Gln Lys Asp Ala Gly Leu Glu Pro Asp Ile
50 55 60
Ala Ile Pro Gly Gln Phe Glu Val Gly Pro Ala Trp Thr Arg Trp Arg
65 70 75 80
Asp Ala Ala Val Gly Cys Lys Thr Cys Arg Arg Leu Leu Ala Arg Asp
85 90 95
Pro Ala Val Pro Ala Ser Leu Trp Ala Asp Ser Arg Thr Gly Glu Ile
100 105 110
Thr Cys Asp Pro Gly Ala Leu Gln Arg Leu Gly Leu Asp Tyr Gly Ala
115 120 125
Pro Pro Leu His Asp Pro Ala Asp Val Gly Cys Arg Arg Cys Trp Lys
130 135 140
Val Leu Arg Glu Thr Pro Ala Gly Glu Trp Ala Asp Ser Ser Gly Ser
145 150 155 160
Ala Ala Cys Pro Glu Ala Arg Arg Pro Gly Pro Pro His Glu Pro Ser
165 170 175
Gly Thr Cys Gln Asp Gly Cys Asp Gly Ala Ala Arg Ala Cys Gly Gly
180 185 190
Pro His Glu Pro Ser Ser Asp Phe Leu Ala Trp Thr Gly Asp Val Phe
195 200 205
Ser Gly Thr Ile Gln Ala Ala Gln Arg Asp Ala Asp Val Ala Trp Lys
210 215 220
Lys Phe Leu Ser Gly Lys Ala Arg Arg Pro Arg Phe Lys Lys Arg Gly
225 230 235 240
Lys Ser Ala Glu Ser Phe Gln Val His Gly Asp Gly Leu Lys Met Asp
245 250 255
Gly Pro Thr Arg Ile Val Met Pro Lys Ile Gly Ala Val Thr Val Met
260 265 270
Ser Asp Asp Ser Met His Pro Ala Met Arg Arg Ser Arg Ser Arg Ala
275 280 285
Gly Ala Gly Arg Arg His Met Gly Asn Arg Arg Arg Ser Arg Ser Leu
290 295 300
Arg Lys His Leu Ala Arg Ser Gly Pro Lys Ala Ala Ala Leu Ala Pro
305 310 315 320
Leu Leu Arg Arg Ala Arg Glu Asp Ala Gly Leu Thr Pro Ala Ala Ala
325 330 335
Leu Ala Ala Leu Asn Phe Glu Ala Gly Ile Tyr Ala Ala Glu Ala Lys
340 345 350
Ala Val Arg Ala Val Arg Ala Ala Lys Gln Glu Gln Gln Leu Leu Gly
355 360 365
Met Leu Asp Ala Glu Glu Arg Thr Leu Pro Val Pro Val Pro Gly Ala
370 375 380
Pro Gln Ala Glu Phe Ala Ala Ile Glu Gly Ile Ala Ala Arg Arg Lys
385 390 395 400
Lys Thr Gln Ala Arg Leu Arg Ser Ala Gln Lys Arg Ala Gly Glu Ser
405 410 415
Pro Asp Ala Glu Ala Leu Arg Thr Ala Ala Trp Thr Ala Pro Lys Leu
420 425 430
Ala Ala Leu Glu Lys Thr Gly Asp Thr Ala Gly Leu Ala Gln Leu Glu
435 440 445
Val Ala Ala Ile Phe Cys Ala Ala Tyr Leu Val Thr Gly Pro Leu Arg
450 455 460
Asp Arg Val Thr Gly Leu Phe Ala Gln Ala Arg Ile Thr Arg Ala Thr
465 470 475 480
Val Thr Leu Gly Ala Asp Gly Leu Trp Trp Cys Ser Ala Gly Ala Glu
485 490 495
Ile Pro Met Glu Ile Arg Thr Arg Pro Ser Arg Arg Gln Arg Glu Gly
500 505 510
Gly Val Ile Gly Leu Asp Phe Gly Val Arg Glu Ile Ala Thr Ala Ser
515 520 525
Asn Gly Val Arg Ile Pro Asn Pro Arg His Tyr Glu Thr Ala Leu Ala
530 535 540
Glu Leu Arg Ala Ala Gln Lys Gln Leu Ser Arg Ser Ala Pro Gly Ser
545 550 555 560
Lys Arg Arg Glu Lys Asp Lys Arg Ala Ala Gly Leu Ile His Ala Asp
565 570 575
Val Ala Arg Leu Arg Ala Asp Ala Ile His Arg Ala Thr Thr Val Leu
580 585 590
Val Arg Gln His Asp Val Ile Ala Ile Glu Gly Trp Asn Val Gln Gln
595 600 605
Val Met Arg Asp Gly Ser Arg Asn Leu Pro Arg Gln Val Arg Arg Arg
610 615 620
Arg Asn Arg Ala Leu Ala Asp Thr Gly Ile Gly Ile Gly Arg Gln Gln
625 630 635 640
Leu Thr Tyr Lys Gly Pro Arg Asn Gly Ala Val Ile Met Ile Thr Ser
645 650 655
Pro Asp Ala Arg Thr Gly Thr Thr Cys Ser Val Asp Gly Lys Ala Arg
660 665 670
Thr Thr Pro Leu Pro Pro Asp Gln Glu Met Phe Thr Ser Asp Arg Cys
675 680 685
Gly His Thr Leu Asp Arg Arg Leu Asn Thr Ala Leu Ala Val Gln Ala
690 695 700
Trp Ala Val Gln Glu Leu Lys Arg Gly Pro Ser Pro Gly Gly Pro Ala
705 710 715 720
Lys Pro Arg Gly Gly Asp Val Arg Pro Gly Thr Ser Arg Ala Arg Gln
725 730 735
Ser Pro Gly Lys Arg Ala Ala Thr Ser Ser Arg Pro Arg Arg Gly Glu
740 745 750
Thr Gly Thr Pro Gly Gly
755
<210> 53
<211> 669
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 53
Met Ala Ala Val Gly Arg Pro Arg His His His Leu His Arg Arg Ala
1 5 10 15
Arg Ser Pro Ser Arg Trp His Arg Gly Ala Arg Leu Arg Leu Leu His
20 25 30
Leu Pro Leu Gly Thr Arg Asp Arg Arg Arg Thr Pro Leu His Ala Arg
35 40 45
Tyr Gly Pro Gly His Arg Ala Leu Arg Arg Gln Arg Gly His Asp Pro
50 55 60
Arg Arg His Ala Arg His Leu Pro Gly Pro Val Pro Pro Gln Pro His
65 70 75 80
Arg Gln Gly Pro His Leu Leu Pro Arg Pro Arg Pro Ala Arg Val His
85 90 95
Arg Ala Pro His Gly Arg Leu His Arg Gln Ala Pro Arg Arg Arg Leu
100 105 110
Thr Thr Ala Cys Gln Arg Pro Pro Leu Pro Cys Val Asp Met Gly Thr
115 120 125
Glu Gly Ala Leu Thr His Arg Val Val Arg Val Cys Leu Asp Asp Ala
130 135 140
Ala Leu Thr Pro Asp Gln Arg Thr Leu Leu Asp Arg His Ala Gly Thr
145 150 155 160
Ala Arg Ala Val Trp Asn Trp Gly Leu Ala Ala Arg Asn Ala Gln Gln
165 170 175
Asp Ala Leu Met Ala His Val Arg Ala Val Ala Leu Glu Gln Ala Ala
180 185 190
Ser Asp Glu Thr Ala Ala Ala Glu Leu Leu Asp Asp Arg Asp Trp Arg
195 200 205
Thr Ala Thr Ile Lys Ala Ala Pro Asp Glu Leu Arg Arg Pro Leu Arg
210 215 220
Ala Ala Thr Leu Gly Arg Ala Phe Thr Ala Glu Thr Arg Asp Pro Asp
225 230 235 240
Ser Arg Phe Ala Trp Trp Ala Val Glu Arg His Gly Val Asn Arg Phe
245 250 255
Ala Val Ser Ser Ser Leu Gln Ala Leu Asp Ala Ala Phe Asp Arg Tyr
260 265 270
Tyr Arg Asp Thr Gly Gly His Arg Ser Ala Arg Arg Ala Arg Pro Arg
275 280 285
Lys Asp Gly Arg Pro Ala Ala Trp Pro Arg Phe Lys Lys Arg Gly Arg
290 295 300
Ala Thr Asp Ala Phe Ala Leu Phe Asn Leu Val Val Ala Gly Gln Asp
305 310 315 320
Pro Trp Arg Val Ile Glu Gly Ala His Arg Ile Lys Val Pro Ser Leu
325 330 335
Gly Ser Leu Arg Val His Glu Asn Thr Lys Arg Leu Arg Arg Leu Ile
340 345 350
Ala Arg Gly Gly Arg Pro Thr Ser Ala Arg Phe Thr Arg Thr Gly Gly
355 360 365
Arg Trp Tyr Met Ser Val Val Val Ala Leu Pro Ala Thr Ala Ala Ser
370 375 380
Thr Val Leu Ser Pro Ala Gly Ala Pro Arg Ala Ala Ala Pro Thr Arg
385 390 395 400
Ala Gln Thr Arg Ala Gly Leu Val Gly Val Asp Leu Gly Ile Lys Thr
405 410 415
Leu Ala Thr Met Ser Asp Gly Thr Leu Val Ala Asn Ala Arg His Gly
420 425 430
Arg Ala Ala Ala Arg Arg Leu Ala Arg Leu Gln Arg Arg Ala Ala Arg
435 440 445
Gln Gln Gly Pro Arg Lys Gly Ala Ala Pro Ser Lys Gly Trp Val Ala
450 455 460
Thr Gln Arg Thr Ile Thr Arg Leu Gln His Asp Thr Ala Ala Arg Arg
465 470 475 480
Arg Gly Leu Val Tyr Glu Leu Thr Lys Thr Leu Ala Ala Gly Tyr Ala
485 490 495
Ala Val Ala Ile Glu Asp Leu Asn Val Ala Gly Met Thr Ala Thr Pro
500 505 510
Ala Ala Arg Pro Asp Pro Asp Arg Pro Gly Val Tyr Leu Ala Asn Gly
515 520 525
Arg Ala Ala Lys Ser Gly Leu Asn Arg Ala Ile Leu Asp Val Gly Phe
530 535 540
Gly Glu Phe Arg Arg Gln Leu Thr Tyr Lys Thr Pro Met Tyr Gly Ala
545 550 555 560
Gln Leu Leu Glu Ile Ala Arg Phe Ala Pro Thr Ser Lys Thr Cys Ser
565 570 575
Thr Cys Gly Ala Val Arg Ala Lys Leu Arg Leu Asp Glu Arg Thr Tyr
580 585 590
Arg Cys Glu His Cys Gly Leu Val Ile Asp Arg Asp Leu Asn Ala Ala
595 600 605
Arg Asn Ile Ala Ala Leu Gly His Gln Ala Leu Gly Thr Ser Pro Ala
610 615 620
Asp Ala Gly Asp Ala Lys Arg Arg Asp His Lys Glu Gly Asp Arg Glu
625 630 635 640
Arg Ser Val Ile Leu Val Ser Gln Asp Leu Gly Pro Pro Arg Ser Ala
645 650 655
Glu Arg Val Thr Asp Ser Ser Pro Pro Thr Arg Ala Ala
660 665
<210> 54
<211> 531
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 54
Met Ala Asp Met Thr Cys Ser Arg Ala Val Leu Leu His Leu Asp Ala
1 5 10 15
Ala Ala Leu Thr Asp Arg Gln Gln Leu Leu Leu Glu Arg Ser Ala Gly
20 25 30
Thr Ser Arg Ala Val Tyr Asn Trp Gly Leu Ala Ala Ile Asn Glu Trp
35 40 45
Glu Thr Arg Gln Tyr Ala Trp Leu Arg Glu Arg Ala His Ala Val Thr
50 55 60
Ser Ala Glu Asp Gln Ala Gln Thr Leu Leu Gly Asp Val Asp Trp Arg
65 70 75 80
Arg Gly Ala Leu Leu Gln Thr Pro Gly Asp Val Arg Arg Arg Pro Asn
85 90 95
Thr Ala Ser Leu Asp Arg Arg Phe Thr Glu Leu Thr Arg Asp Pro Asn
100 105 110
Ser Arg Phe His Trp Trp His Val Glu Asn His Gly Val Pro Arg His
115 120 125
Ala Met Ser Thr Ala Leu Gly Asn Leu Asp Thr Ala Ile Asp Arg Tyr
130 135 140
Arg Asn His Val Gly Pro Ser Ser Arg Pro Pro Arg Lys Pro Arg Lys
145 150 155 160
Asp Gly Arg Pro Ala Gly Trp Pro Arg Phe Lys Lys Lys Gly Arg Ser
165 170 175
Lys Asp Ser Phe Ala Ile Phe Asn Leu Ser Val Thr Gly Arg Arg Thr
180 185 190
Asp Pro Trp Lys Val Ile Asp Lys Gly His Arg Ile Arg Val Pro Asn
195 200 205
Leu Gly Ser Leu Arg Val His Glu Asn Thr Arg Arg Leu Arg Arg Trp
210 215 220
Ile Gln Arg Gly Ala Ile Pro Lys Ser Ala Arg Phe Val Arg Arg Gly
225 230 235 240
Thr Gly Trp Thr Val Ser Ile Thr Leu Asp Val Pro Ala Glu Val Ile
245 250 255
Ser Ala Pro Ala Thr Thr Arg Arg Gln Arg Ala Ala Gly Ala Val Gly
260 265 270
Val Asp Val Gly Val His Ser Leu Val Ala Leu Ser Thr Asp Glu Leu
275 280 285
Ile Ala Gly Cys Pro Ser Ala Asp Phe Trp Arg Arg Leu Arg Arg Leu
290 295 300
Gln Arg Arg Ala Ala Arg Gln Arg Gly Pro Thr His Glu Gln Gly Pro
305 310 315 320
Ser Ala Gly Trp Val Glu Thr Thr Arg Arg Ile Ala Arg Leu Gln Arg
325 330 335
Val Asp Ala Val Arg Arg Arg Gly Arg Leu His Glu Val Thr Lys Arg
340 345 350
Ile Ala Thr Arg Phe Glu Thr Val Gly Ile Glu Asp Leu Ala Val Ala
355 360 365
Gly Met Thr Ala Ala Pro His Pro Lys Pro Asp Pro Asp His Pro Gly
370 375 380
Ser Phe Leu Pro Asn Gly Arg Arg Ser Lys Ala Gly Leu Asn Arg Ser
385 390 395 400
Ile Arg Arg Ala Ala Phe Gly Glu Ile Arg Arg Gln Leu Leu Tyr Lys
405 410 415
Thr Ser Trp Tyr Gly Ala Arg Leu Val Ile Val Asp Arg Phe Ala Pro
420 425 430
Thr Ser Lys Thr Cys Ser Gly Cys Ser Ala Val Asn Ala Thr Leu Arg
435 440 445
Leu Ser Glu Arg Val Tyr His Cys Glu Gln Cys Gly Leu Val Leu Asp
450 455 460
Arg Asp Leu Asn Ala Ala Arg Asn Ile Arg Ser Leu Ala Leu Thr Pro
465 470 475 480
Ser Pro Val Asp Thr Ala Asp Val Lys Arg Cys Asp Thr Pro Thr Pro
485 490 495
Ser Arg Ser Arg Pro Ala Arg Cys Trp Gly Pro Ala Asp Leu Pro Lys
500 505 510
Gly Arg Pro Gln Arg Pro Ala Met Asp Leu Pro Ser Pro Leu Arg Asp
515 520 525
Ser Gly Asn
530
<210> 55
<211> 527
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 55
Met Ala Asp Met Thr Cys Ser Arg Ala Val Leu Leu His Leu Asp Ala
1 5 10 15
Ala Ala Leu Thr Asp Arg Gln Gln Leu Leu Leu Glu Arg Ser Ala Gly
20 25 30
Thr Ser Arg Ala Val Tyr Asn Trp Gly Leu Ala Thr Ile Asn Glu Trp
35 40 45
Glu Ala Arg Gln Tyr Ala Trp Leu Arg Glu Arg Ala Arg Ala Val Thr
50 55 60
Arg Thr Glu Gly Glu Ala Gln Thr Leu Leu Gly Asp Val Asp Trp Arg
65 70 75 80
Arg Gly Ser Leu Leu Gln Ala Pro Gln Asp Leu Arg Arg Arg Pro Asn
85 90 95
Arg Thr Ser Leu Gly Arg Arg Phe Thr Glu Leu Thr Arg Asp Pro Asn
100 105 110
Ser Arg Phe His Trp Trp His Ala Glu Asn His Gly Val Pro Arg His
115 120 125
Ala Met Ser Thr Ala Leu Gly Asn Leu Asp Thr Ala Ile Val Arg Tyr
130 135 140
Trp Asp Arg Ala Arg Ser Pro Asp Arg Pro Pro Arg Lys Pro Arg Lys
145 150 155 160
Asp Gly Arg Pro Ala Gly Trp Pro Arg Phe Lys Lys Lys Gly Arg Ser
165 170 175
Lys Asp Ser Phe Ala Ile Phe Asn Leu Ser Val Thr Gly Arg Arg Thr
180 185 190
Asp Pro Trp Lys Val Ile Glu Gln Gly His Arg Ile Arg Val Pro Asn
195 200 205
Leu Gly Ser Leu Arg Val His Glu Asn Thr Arg Arg Leu Arg Arg Trp
210 215 220
Ile Gln Arg Gly Ala Ile Pro Lys Ser Val Arg Phe Val Arg Arg Gly
225 230 235 240
Thr Gly Trp Thr Val Ser Ile Val Leu Asp Val Pro Ala Glu Val Ile
245 250 255
Ser Ala Pro Val Thr Thr Arg Arg Gln Arg Thr Ala Gly Ala Val Gly
260 265 270
Val Asp Val Gly Val His Ser Leu Val Ala Leu Ser Thr Asp Glu Leu
275 280 285
Thr Ala Gly Tyr Pro Ser Ala Ala Phe Trp Gln Arg Leu Arg Arg Leu
290 295 300
Gln Arg Arg Ala Ala Arg Gln Arg Gly Pro Thr His Glu Gln Gly Pro
305 310 315 320
Ser Ala Gly Trp Val Glu Thr Thr Arg Arg Ile Ala Arg Leu Gln Arg
325 330 335
Val Asp Ala Val Arg Arg Arg Gly Arg Leu His Glu Val Thr Lys Arg
340 345 350
Ile Ala Ile Gly Phe Glu Thr Val Gly Ile Glu Asp Leu Ala Val Ala
355 360 365
Gly Met Thr Ala Ala Pro His Pro Lys Pro Asp Pro Asp His Pro Gly
370 375 380
Ser Phe Leu Pro Asn Ser Arg Arg Ser Lys Ala Gly Leu Asn Arg Ser
385 390 395 400
Ile Arg Arg Ala Gly Phe Arg Glu Leu Arg Arg Gln Leu His Tyr Lys
405 410 415
Thr Ser Trp Tyr Gly Ala Cys Leu Val Ile Val Asp Arg Phe Val Pro
420 425 430
Thr Ser Lys Thr Cys Ser Gly Cys Gly Ala Val Lys Ala Thr Leu Arg
435 440 445
Leu Ser Glu Arg Val Tyr His Cys Glu Gln Cys Gly Leu Val Leu Asp
450 455 460
Arg Asp Leu Asn Ala Ala Arg Asn Ile Arg Ser Leu Ala Leu Thr Pro
465 470 475 480
Ser Pro Val Asp Thr Ala Asp Val Lys Arg Cys Asp Thr Pro Thr Pro
485 490 495
Ser Arg Ser Arg Pro Ala Arg Cys Trp Gly Pro Ala Asp Leu Pro Lys
500 505 510
Gly Arg Pro Thr Glu Ala Ser Asn Gly Pro Pro Ala Pro Thr Pro
515 520 525
<210> 56
<211> 560
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 56
Met Ala Lys Lys Thr Ser Thr Asp Ala Asp Thr Met Asn Arg Ala Tyr
1 5 10 15
Lys Phe Arg Leu Asp Pro Asp Gln Ala Gln Lys Ala Glu Leu Met Arg
20 25 30
Cys Val Gly Ala Ala Arg Tyr Thr Tyr Asn Leu Leu Thr Thr His Asn
35 40 45
Leu Glu Val Ser Arg Ala Arg Asp Asp Tyr Trp His Ala Arg Ile Glu
50 55 60
Ala Gly Val Asp Glu Ala Thr Val Lys Ala Glu Leu Lys Lys Leu Glu
65 70 75 80
Lys Lys Asp Pro Thr Tyr Lys Ile Leu Tyr Lys Ser Gly Tyr Arg Pro
85 90 95
Leu Tyr Leu Thr Pro Glu Val Lys Arg His Arg Ala Ala Gly Glu Ala
100 105 110
Ile Val Ala Gly Ala Asp Pro Ala Val Val Trp Pro Glu Thr Glu Arg
115 120 125
Phe Ser Glu Pro Trp Leu His Thr Ile Lys Cys Arg Val Leu Asp Ser
130 135 140
Gly Leu Met Asn Ala Glu Lys Ala Trp Lys Asn Phe Phe Glu Ser Arg
145 150 155 160
Ala Gly Ala Arg Ala Gly Ala Gln Met Gly Leu Pro Arg Arg Lys Arg
165 170 175
Lys Ser Ser Gly Arg Asp Ser Phe Thr Ile His Ala Pro Glu Val Ile
180 185 190
Gly Ala Tyr Gly Thr Pro Tyr Lys Lys Ser Glu Pro Glu Tyr Val Arg
195 200 205
Arg Lys Lys Gln Leu Lys Cys Arg Gly Ile Ser Ala Asp Pro Thr Ile
210 215 220
Glu Asp Tyr Arg His Val Arg Leu Ser His Leu Gly Val Ile Arg Thr
225 230 235 240
His Asn Thr Thr Lys Pro Leu Val Lys Ala Met Arg Ala Gly Ala Gln
245 250 255
Ile Arg Ser Tyr Thr Val Ser Arg Ala Ala Asp Arg Trp Tyr Val Ser
260 265 270
Ile Leu Val Glu Leu Thr Arg Pro Ser Thr Asn Pro Thr Arg Ala Gln
275 280 285
Arg Ser Ala Gly Ala Val Gly Val Asp Leu Gly Val Arg Tyr Leu Ala
290 295 300
Ala Leu Ser Asp Glu Gln Ala Pro Gln Arg Phe Ala Gln Tyr Pro Ser
305 310 315 320
Leu Glu Phe Thr Gly Asp Gly Ala Pro Thr Leu Ala Asn Pro Arg Trp
325 330 335
Ala Arg Thr Ala Glu Lys Arg Leu Val Arg Leu Gln Arg Ala Leu Ala
340 345 350
Arg Ala Gln Lys Gly Ser Asn Arg Arg Thr Arg Ile Val Gln Gln Ile
355 360 365
Ala Arg His His His Leu Val Ala Leu Arg Arg Glu Ser Gly Leu His
370 375 380
Gln Val Ser Lys Arg Leu Ser Thr Gly Tyr Thr Leu Ile Gly Leu Glu
385 390 395 400
Asp Leu Ala Val Ala Gly Met Thr Ala Ser Ala Ala Gly Thr Ile Glu
405 410 415
Ala Pro Gly Lys Asn Val Arg Gln Lys Ala Gly Leu Asn Arg Ser Ile
420 425 430
Leu Asp Ala Ala Phe Ser Thr Leu Arg Arg Gln Leu Glu Tyr Lys Ala
435 440 445
Ser Trp Tyr Gly Ser Gln Val Arg Ile Ile Asp Arg Phe Phe Ala Ser
450 455 460
Ser Gln Thr Cys Ser Ala Cys Gly Ala Arg Ala Lys Thr Lys Leu Gly
465 470 475 480
Leu His Val Arg Val Phe Glu Cys Ala Ala Cys Gly Val Arg Ile Asp
485 490 495
Arg Asp Val Asn Ala Ala Arg Asn Ile Arg Ala Glu Ala Val Arg Met
500 505 510
His Glu Ala Gln Gln Leu Ala Pro Gly Thr Gly Glu Ser Leu Asn Gly
515 520 525
Arg Gly Val Ala Asp Ser Asp Ala Ala Val Ser Val Val Leu Gly Asp
530 535 540
Val Ala Leu Asp Val Ser Arg Pro Ala Ala Thr Gly Gly Gly Ser Pro
545 550 555 560
<210> 57
<211> 496
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 57
Met Ala Gln Val Leu Lys Ala Phe Arg Tyr Ala Leu Asp Pro Thr Ser
1 5 10 15
Ala Gln Val Glu Leu Leu Arg Arg Trp Ala Gly Asn Ser Arg Leu Ala
20 25 30
Phe Asn Phe Ala Leu Ala Met Lys Gln Asp Ala His Gln Arg Trp Arg
35 40 45
Asp Glu Leu Asp Thr Leu Leu Ala Gln Gly Leu Glu Ala Lys Glu Ala
50 55 60
Arg Ala Arg Leu Lys Gly Thr His Lys Ile Pro Thr Lys Pro Ala Val
65 70 75 80
Tyr Lys Ala Phe Gln His Val Arg Gly Asp Glu Ala Lys Gly Ile Asp
85 90 95
Gly Ile Ala Pro Trp His Ala Glu Val Ser Thr Tyr Val Phe Gln Ser
100 105 110
Ala Phe Asp Asp Ala Asp Arg Ala Trp Lys Asn Trp Leu Asp Ser Tyr
115 120 125
Thr Gly Lys Arg Ala Gly Arg Arg Val Gly Tyr Pro Arg Phe Lys Lys
130 135 140
Lys Phe Arg Ser Arg Asp Ser Phe Arg Leu His His Asp Ala Lys Lys
145 150 155 160
Pro Lys Pro Ala Leu Arg Leu Glu Gly Tyr Arg Arg Leu Arg Leu Gly
165 170 175
Gly Ala Leu Gln Thr Val Arg Leu His Gly Ser Ala Lys Thr Leu His
180 185 190
Arg Leu Val Ser Ser Gly Arg Ala Val Val Gln Ser Val Thr Val Ser
195 200 205
Arg Gly Gly Ser Arg Trp Tyr Ala Ser Val Leu Cys Lys Val Glu Val
210 215 220
Asp Pro Ser Thr Pro Thr Arg Arg Gln Lys Ala Asn Gly Arg Val Gly
225 230 235 240
Leu Asp Trp Gly Leu Gly His Leu Ala Ala Leu Ser Lys Pro Leu Asp
245 250 255
Gly Ser Arg Leu Val Asp Asn Pro Arg His Leu Arg Lys Ala Ser Glu
260 265 270
Arg Leu Leu Lys Ala Gln Arg Ala Leu Ser Arg Thr Gln Lys Gly Ser
275 280 285
Ala Arg Arg Arg Lys Ala Ala Val Arg Val Gly Arg Leu His His Arg
290 295 300
Val Ala Glu Gln Arg Ala Thr Phe Leu His Thr Leu Ser Lys Arg Leu
305 310 315 320
Thr Thr Thr Phe Ala Cys Val Ala Ile Glu Asp Leu Asn Val Ser Gly
325 330 335
Met Thr Arg Ser Ala Arg Gly Thr Val Arg Lys Pro Gly Arg Asn Val
340 345 350
Arg Ser Lys Ala Gly Leu Asn Arg Ser Ile Leu Asp Ala Ala Pro Ala
355 360 365
Glu Leu Arg Arg Gln Leu Glu Tyr Lys Ala Ser Trp Tyr Gly Ser Glu
370 375 380
Val Ala Ile Leu Asp Arg Trp Phe Pro Ser Ser Lys Thr Cys Ser Gly
385 390 395 400
Cys Gly Trp Arg Asn Pro Ser Leu Ala Leu Ser Glu Arg Glu Phe Val
405 410 415
Cys Ala Glu Cys Gly Leu Arg Met Asp Arg Asp Leu Asn Ala Ala Asp
420 425 430
Asn Ile Ala Ala His Ala Val Ala Pro Asp Ser Gly Ala Gly Ala Pro
435 440 445
Gly Arg Gly Glu Ser Val Asn Ala Arg Gly Gly Gly Val Thr Pro Gly
450 455 460
Leu Leu Arg Glu Pro Gly Pro Leu Pro Leu Lys Arg Glu Gly Ala Val
465 470 475 480
Ser Ser Gly Thr Ala Pro Pro Arg Arg Gly Asp Pro Pro Thr Phe Pro
485 490 495
<210> 58
<211> 500
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 58
Met Ala Arg Gln Glu Val Leu Arg Ala Phe Arg Phe Ala Leu Asp Pro
1 5 10 15
Thr Pro Ala Gln Glu Glu Thr Leu Arg Arg Tyr Ala Gly Ala Ala Arg
20 25 30
Trp Ala Phe Asn His Ala Leu Ala Ala Lys Val Ala Ala His Glu Glu
35 40 45
Trp Arg Asn Gln Ile Ala Ala Ile Val Glu Thr Gly Val Pro Glu Asp
50 55 60
Val Ala Arg Lys Gln Val Lys Val Pro Ile Pro Thr Lys Pro Ala Ile
65 70 75 80
Gln Lys Ala Trp Asn Gln Val Lys Gly Asp Ser Arg Thr Gly Glu Asp
85 90 95
Gly Cys Cys Pro Trp Trp His Glu Val Asn Thr Tyr Cys Phe Gln Ser
100 105 110
Ala Phe Val Asp Ala Asp Ala Ala Trp Lys Asn Trp Leu Asp Ser Leu
115 120 125
Thr Gly Arg Arg Ala Gly Arg Arg Val Gly Tyr Pro Arg Phe Lys Lys
130 135 140
Lys Gly Arg Ser Arg Asp Ser Phe Arg Ile His His Asp Val Lys Lys
145 150 155 160
Pro Gly Ile Arg Leu Val Thr Tyr Arg Arg Leu Arg Ile Pro Lys Leu
165 170 175
Gly Glu Val Arg Leu His Asp Ser Ala Lys Arg Leu Ala Arg Leu Ile
180 185 190
Asp Arg Ser Glu Ala Ile Val Gln Ser Val Thr Val Ser Arg Gly Gly
195 200 205
His Arg Trp Tyr Ala Ser Val Leu Cys Lys Val Thr Val Glu Leu Pro
210 215 220
Asp Arg Pro Thr Arg Arg Gln Ala Ala Ala Gly Thr Ile Gly Val Asp
225 230 235 240
Leu Gly Val Thr His Leu Met Ala Leu Ser Gln Glu Val Asp Leu Gly
245 250 255
Gln Gly Pro Thr Gln Leu Val Asp Asn Pro Arg Tyr Leu Lys Ala Thr
260 265 270
Leu Arg Arg Leu Arg Arg Ala Gln Gln Ala Leu Ser Arg Thr Glu Lys
275 280 285
Gly Ser Ala Arg Arg Glu Lys Ala Lys Arg Arg Val Ala Arg Leu His
290 295 300
Gly Gln Val Ala Glu Arg Arg Ala Thr Phe Leu His Gly Ile Thr Lys
305 310 315 320
Arg Leu Ala Thr Gly Phe His Thr Val Ala Ile Glu Asp Leu Asn Val
325 330 335
Thr Gly Met Thr Arg Ser Ala Lys Gly Thr Ile Glu Ala Pro Gly Lys
340 345 350
Asn Val Arg Ala Lys Ala Gly Leu Asn Arg Ala Ile Leu Asp Ala Ser
355 360 365
Pro Gly Glu Ile Arg Arg Gln Leu Thr Tyr Lys Thr Ser Trp Tyr Gly
370 375 380
Ser Ala Leu Ala Ile Cys Asp Arg Trp Phe Pro Ser Ser Arg Thr Cys
385 390 395 400
Ser Ala Cys Gly Glu Gln Asn Pro Arg Leu Thr Leu Ala Asp Arg Val
405 410 415
Phe Val Cys Pro Ser Cys Gly Leu Ala Met Asn Arg Asp Ile Asn Ala
420 425 430
Ala Arg Asn Ile Ala Val His Ala Val Ile Ala Pro Thr Ser Gly Ala
435 440 445
Val Ala Ser Gly Lys Gly Glu Thr Leu Asn Ala Arg Gly Ala Ser Val
450 455 460
Ser Pro Ser Ile Pro Gln Gly Arg Gly Arg Glu Ala Val Lys Arg Glu
465 470 475 480
Asp Ala Gly Ser Ser Gly Ser Ala Pro Pro Arg Arg Ser Asn Pro Pro
485 490 495
Ala Leu Pro Pro
500
<210> 59
<211> 539
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 59
Met Ala Arg Arg Glu Val Leu Lys Ala Phe Arg Phe Thr Leu Asp Pro
1 5 10 15
Thr Arg Ala Gln Glu Thr Glu Leu Arg Arg Tyr Ala Gly Ala Ala Arg
20 25 30
Trp Ala Phe Asn His Ala Leu Ala Ala Lys Val Ala Ala His Gln Glu
35 40 45
Arg Lys Gln Arg Ile Ala Ala Leu Ile Gly Gly Gly Leu Ala Glu Glu
50 55 60
Ala Ala Arg Lys Gln Ile Lys Val Ser Val Leu Gly Lys Gly Val Ile
65 70 75 80
Gln Lys Ala Trp Val Arg Ala Arg Gly Ser Ser Arg Asn Gly Ala Glu
85 90 95
Gly Ile Cys Pro Trp Ser His Glu Val Asn Asn Tyr Cys Phe Gln Ser
100 105 110
Ala Phe Asp Asp Ala Asp Ala Ala Trp Lys Asn Trp Leu Asp Ser Ile
115 120 125
Thr Gly Lys Arg Val Gly Arg Trp Val Gly Tyr Pro Arg Phe Lys Lys
130 135 140
Lys Gly Arg Thr Arg Asp Ser Phe Arg Ile Pro His Asn Val Asn Lys
145 150 155 160
Pro Gly Ile Arg Leu Val Thr His Arg Arg Leu Arg Ile Pro Lys Leu
165 170 175
Gly Glu Val Arg Leu His Asp Ser Ala Arg Arg Leu Val Arg Leu Ile
180 185 190
Lys Cys Gly Gln Ala Val Val Arg Ser Val Thr Val Ser Arg Gly Gly
195 200 205
His Arg Trp Tyr Ala Ser Val Leu Cys Lys Val Thr Val Glu Leu Pro
210 215 220
Asp Lys Pro Thr Arg Arg Gln Ala Ala Ala Gly Thr Ile Gly Leu Asp
225 230 235 240
Leu Gly Val Thr His Leu Ile Ala Leu Ser Gln Pro Ala Asp Leu Gly
245 250 255
Lys Gly Pro Thr Gln Leu Leu Asp Asn Pro Arg Tyr Leu Arg Ala Thr
260 265 270
Thr Lys Arg Leu Arg Arg Ala Gln Gln Ala Leu Ser Arg Thr Gln Lys
275 280 285
Gly Ser Thr Arg Arg Glu Lys Ala Lys Arg Arg Val Ala Arg Leu His
290 295 300
Asn Gln Val Ala Ala Arg Arg Ala Thr Phe Leu His Gly Val Thr Lys
305 310 315 320
Arg Leu Ala Thr Gly Phe His Ile Val Ala Val Glu Asp Leu Asn Val
325 330 335
Ala Gly Met Thr Arg Ser Ala Arg Gly Thr Val Gln Asn Pro Gly Arg
340 345 350
Lys Val Arg Gln Lys Ala Gly Leu Asn Arg Pro Ile Leu Asp Ala Ser
355 360 365
Pro Ala Glu Ile Arg Arg Gln Leu Gln His Lys Thr Ser Trp Tyr Ser
370 375 380
Ser Thr Ile Ala Val Ala Asp Arg Trp Phe Pro Ser Ser Lys Thr Cys
385 390 395 400
Ser Ala Cys Gly Arg Gln Asn Pro Arg Leu Thr Leu Ala Asp Arg Val
405 410 415
Ser Thr Cys Ser Ser Cys Gly Leu Thr Met Asn Arg Asp Ile Asn Ala
420 425 430
Ala Arg Asn Ile Ala Val Cys Ala Val Ala Arg Pro Gly Val Gln Pro
435 440 445
Thr Gly Ala Gly Ser Asp Pro Arg Asn Val Asn Val Lys Ala Pro Ala
450 455 460
Thr Gly Ala Phe Thr Thr Met Ala Ile Phe Gln Ser Leu Ser Ala His
465 470 475 480
Gly Leu Ser Thr Asp Arg Ala Asn Ala Ser Ile Met Ser Ile Pro Gly
485 490 495
Val Met Pro Arg Ala Arg Ser Ser Ile Pro Ala Pro Ala Cys Ala Ile
500 505 510
Pro Ala Arg Ser Ala Thr Cys Gly Leu Arg Pro Arg Ala Cys Arg Ser
515 520 525
Ser Cys Thr Ser Leu Thr Ser Arg Ala Ala Ser
530 535
<210> 60
<211> 595
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 60
Met Ala Thr Ala Glu Thr Glu Ala Pro Ala Pro Val Leu Arg Ala Tyr
1 5 10 15
Arg Phe Ala Leu Asp Pro Thr Gln Ala Gln Leu Ala Asp Phe Ala Arg
20 25 30
His Ala Gly Ala Thr Arg Trp Ala Thr Asn Arg Gly Ile Ala Trp Met
35 40 45
Ser Gln Ala Gln Leu Ala Trp Glu Ala Arg Arg Asp Glu Val Ile Thr
50 55 60
Arg Ile Ala Gly Pro Met Pro Gln Pro Pro Asp Gly Leu Asp Glu Lys
65 70 75 80
Ser Thr Ala Ala Trp Thr Ala Gln Val Arg Gly Glu Arg Ile Lys Gln
85 90 95
Trp Asp Lys Gln Ala Arg Pro Ile Leu Lys Ala Glu Ala Ala Glu Leu
100 105 110
Ala Ala Asp Thr Lys Arg Leu Asp Asp Glu Arg Lys Ala Leu Met Ala
115 120 125
Ala Val Lys Asp Lys Thr Arg Pro Asp Leu Leu Glu Ile Lys Glu Arg
130 135 140
Leu Ala Ala Val Arg Arg Glu Val Leu Gln Phe Lys Lys Ala Arg Phe
145 150 155 160
Glu Arg Gly Asp Ala Ile Pro Ser Ala Met Asp Cys Thr Thr Met Trp
165 170 175
Arg Glu Ile Arg Asp Gln Asp Ala Ala Asp Gly Gly Ser Pro Trp His
180 185 190
Pro Glu Val Ser Val Tyr Cys Phe Thr Asn Gly Phe Asp Arg Ala Gln
195 200 205
Ala Ala Met Gln Asn Trp Met Ala Ser Arg Thr Gly Ala Arg Val Gly
210 215 220
Arg Ala Met Gly Arg Pro Arg Phe Lys Lys Lys Gly Arg Ser Thr Asp
225 230 235 240
Ser Phe Thr Leu Phe His Asp Val His Asn Pro Ser Ile Arg Pro Asp
245 250 255
Gly His Arg Arg Leu Asn Val Pro Arg Ile Gly Ser Val Arg Leu Phe
260 265 270
Glu Ser Cys Lys Pro Leu Ala Arg Leu Ile Asp Arg Gly His Ala Val
275 280 285
Val Lys Ser Val Thr Ile Ser Arg Gly Gly Ser Arg Trp Tyr Ala Ser
290 295 300
Val Leu Cys Glu Leu Ser Pro Glu Ala Met Arg His Arg Glu Glu Val
305 310 315 320
Ser Arg Ala Arg Gln Lys Val Ser Lys Glu Arg Gln Arg Met Arg Pro
325 330 335
Ala Val Gly Val Glu Trp Gly Val Arg Thr Leu Val Ala Leu Ser Asp
340 345 350
Glu Thr Ala Tyr Glu Asn Pro Arg Tyr Gly Lys Arg His Lys Arg Ala
355 360 365
Leu Val Lys Ala Ser Gln Ala Val Ala Arg Lys Pro Phe Val Arg Gly
370 375 380
Gln Ala Gln Ser Ala Asn Arg Ala Lys Ala Val Arg Arg Leu Gly Arg
385 390 395 400
Val His His Arg Thr Ala Glu Ala Arg Arg Gly Thr Leu Asn Gln Ile
405 410 415
Ser Ala Ser Ile Ala Ile Asn Tyr Pro Glu Ile Ala Val Arg Asp Leu
420 425 430
Asn Val Arg Gln Met Thr Gly Ser Ala Lys Gly Thr Val Glu Ala Pro
435 440 445
Gly Ser Asp Val Lys Val Lys Ala Gly Phe Asn Arg Ala Ile Leu Asp
450 455 460
Thr Ala Pro Ala Glu Leu Arg Arg Gln Leu Glu Tyr Lys Ala Ala Gln
465 470 475 480
Trp Gly Thr Arg Phe Arg Ala Ala Ala Gly Asp Leu Pro Ser Ser Gln
485 490 495
Thr Cys Cys Lys Cys Gly Trp Arg Asp Pro Ser Ile Pro Leu Thr Gln
500 505 510
Val Val Phe Arg Cys Gly Asn Ile Lys Cys Gly Met Lys Leu Asp Arg
515 520 525
Glu Val Asn Ala Ala Arg Asn Ile Leu His His Ala Val Pro Leu Ala
530 535 540
Ser Asp Ile Gly Glu Ser Leu Asn Ala Cys Gly Gly Gly Gln Ser Pro
545 550 555 560
Ser Gly Asp Arg Arg Leu Ser Thr Pro Leu Ser Lys Gln Glu Gly Gln
565 570 575
Ser Arg Arg Arg Gly Gly Ser Ser Arg Gly Ser Asp Pro Pro Ala Ser
580 585 590
Leu Asp Ser
595
<210> 61
<211> 631
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 61
Met Ala Thr Cys Gly Pro Ala Gln Arg Leu Gly Thr Gly Gly Thr Pro
1 5 10 15
Arg Val Ala Ala Lys Ser Asn Pro Ala Gly Pro Gly Pro Gly Pro Gly
20 25 30
Pro Ala Ala Val Thr Met Pro Gly Thr Glu Val Leu Arg Ala Tyr Arg
35 40 45
Phe Thr Leu Asp Pro Ser Asp Ala Glu Arg Ala Ala Leu Ser Arg Tyr
50 55 60
Ala Gly Ala Cys Arg Trp Ala Tyr Asn Tyr Ala Val Ala Lys Lys Thr
65 70 75 80
Gln Ala His Gln Ala Trp Ala Asp Arg Arg Ala Ala Tyr Leu Glu Ala
85 90 95
Gly Leu Ser Glu Ala Glu Ala Lys Glu Arg Ile Lys Ala Asp Gly Ala
100 105 110
Glu Leu Thr Asp Arg Ile Lys Val Trp Asp His His Arg Lys Ser Leu
115 120 125
Thr Leu Ile Leu Ala Gly Lys Pro Pro Leu Pro Ala Met Gln Pro Pro
130 135 140
Ala Gly Gln Glu Ala Leu Val Arg Gln Leu Ala Ala Ala Arg Ala Asp
145 150 155 160
Ala Ala Gly Thr Ser Arg Glu Arg Glu Leu Leu Thr Glu Gly Arg Ala
165 170 175
Thr Val Asn Ala Leu Lys Ala Lys Ala Phe Ser Ala Gly Phe Arg Thr
180 185 190
Pro Asn Ala Thr Asp Thr Ser Ala Leu Trp Arg Met Glu Arg Asp Leu
195 200 205
Pro Lys Glu Glu Gly Gly Ser Pro Trp Trp Arg Glu Val Asn Val Tyr
210 215 220
Cys Phe Thr Ser Gly Phe Asp Arg Ala Gln Val Ala Trp Lys Tyr Trp
225 230 235 240
Gln Asp Ser Leu Ala Gly Arg Arg Ala Gly Gln Arg His Gly Tyr Pro
245 250 255
Arg Phe Lys Lys Lys Gly His Ala Glu Ser Phe Ala Leu Phe His Asp
260 265 270
Val Lys Arg Pro Ile Ile Arg Leu Glu Gly Tyr Arg Arg Leu Val Met
275 280 285
Pro Gly Leu Gly Ser Ile Arg Ile His Asp Ser Gly Lys Arg Leu Ala
290 295 300
Arg Leu Val Glu Arg Gly Gln Ala Val Ile Gln Ser Val Thr Val Thr
305 310 315 320
Arg Gly Gly His Arg Trp Tyr Ala Ser Val Leu Ala Lys Val Gln Gln
325 330 335
Asp Ala Pro Val Leu Trp Glu His Val His Asp Asp Gly Ile Arg Thr
340 345 350
Pro Tyr Leu Ser Arg Ala Gln Ala Glu Lys Ala Ala Glu Asn Gly Gly
355 360 365
Arg Val Glu Gln Ile Gly Arg Pro Thr Ala Arg Gln Arg Ala Gly Gly
370 375 380
Leu Val Gly Val Asp Leu Gly Ser Arg Asn Leu Ala Ala Leu Ser Ser
385 390 395 400
Pro Leu Asp Pro Ala Asp Pro Ala Asp Pro Ala Leu Val Gln Tyr Pro
405 410 415
Arg Leu Leu Ala Asp Ser Leu Ala Lys Leu Ser Lys Ala Gln Arg Ala
420 425 430
Met Ser Arg Cys Gln Gln Gly Ser Gly Arg Trp Arg Lys Ala Thr Ala
435 440 445
Arg Val Ser Arg Val His Gln Gln Ile Thr Val Arg Arg Ala Ser Phe
450 455 460
Leu His Gly Leu Ser Lys Lys Leu Ala Thr Gly Phe Thr His Val Ala
465 470 475 480
Ile Glu Asp Leu Asp Leu Thr Ala Leu Thr Thr Ser Ala Lys Gly Thr
485 490 495
Arg Asp Lys Pro Gly Lys Asn Val Lys Ala Lys Ala Arg Leu Asn Arg
500 505 510
His Leu Leu Asp Ala Gly Leu Gly Ser Leu Arg Lys Lys Leu Ala Tyr
515 520 525
Lys Thr Ala Trp Tyr Gly Ser Gln Leu Val Val Leu Asp Gln Gly Glu
530 535 540
Pro Val Ile Ala Thr Cys Ala Lys Cys Lys Glu Arg Asn Pro Ser Ser
545 550 555 560
Asp Pro Ser Cys Ser Gln Phe His Cys Pro Ser Cys Gly Ala Ala Val
565 570 575
His Arg His Glu Asn Ser Ala Ala Asn Ile Val Asp Ala Ala Arg Arg
580 585 590
Gln Leu Lys Thr Val Ala Ser Asp Arg Gly Glu Thr Gln Asn Ala Arg
595 600 605
Arg Ala Pro Ala Ser Pro Arg Val Arg Lys Ala Pro Gly Gln Gly Ala
610 615 620
Leu Lys Arg Glu Asp Thr Gly
625 630
<210> 62
<211> 505
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 62
Met Ala Thr Val Leu Arg Ala Phe Lys Phe Ala Leu Asp Pro Thr Ala
1 5 10 15
Asp Gln Arg Thr Lys Leu Lys Arg Tyr Ala Gly Ala Ala Arg Arg Gly
20 25 30
Tyr Asn Phe Ala Leu Gly Met Lys Ile Gln Ala His Gln Gln Trp Arg
35 40 45
Glu Arg Val Asp Ala Leu Val Ala Glu Arg Met Glu Glu Ser Glu Ala
50 55 60
Arg Arg His Cys Gly Ile Ala Ile Pro Arg Lys Pro Ala Val Tyr Lys
65 70 75 80
Glu Phe Ile Ala Thr Arg Gly Asp Asp Arg Thr Gly Glu Asp Gly Ile
85 90 95
Ala Pro Trp His His Gln Ile Pro Ser Tyr Cys Phe Gln Ser Ala Phe
100 105 110
Asn Asp Ala Asp Gln Ala Trp Lys Asn Trp Leu Asp Ser Leu Thr Gly
115 120 125
Lys Arg Ala Gly Arg Arg Val Gly Tyr Pro Arg Phe Lys Ser Lys His
130 135 140
Arg Ser Arg Asp Ser Phe Arg Leu His Asn Lys Lys Gly Ala Thr Pro
145 150 155 160
Met Leu Arg Leu Glu Gly Tyr Arg Arg Leu Asn Leu His Thr Lys Leu
165 170 175
Gly Ser Val Arg Leu His Asp Ser Gly Lys Arg Met His Arg Leu Val
180 185 190
Ala Ser Gly Ala Ala Val Val Lys Ser Val Thr Val Ser Arg Gly Gly
195 200 205
Ser Arg Trp Tyr Ala Ser Val Leu Cys Glu Val Glu Thr Asp Leu Pro
210 215 220
Ala Pro Thr Arg Arg Gln Arg Arg Asn Gly Arg Val Gly Val Asp Leu
225 230 235 240
Gly Thr His His Leu Ala Ala Leu Ser Lys Pro Val Glu Gly Ala Val
245 250 255
Leu Ile Asp Asn Pro Arg His Val Arg Ala Ala Ala Lys Arg Leu Ala
260 265 270
Lys Ala Gln Arg Ala Leu Ala Arg Thr Glu Lys Gly Ser Ala Arg Arg
275 280 285
Lys Lys Ala Ala Val Arg Val Gly Arg Leu Tyr His Ala Val Ala Leu
290 295 300
Lys Arg Ala Thr Thr Leu His Ala Leu Ser Lys Arg Leu Val Thr Thr
305 310 315 320
Phe Ala Cys Val Ala Val Glu Asp Leu Asn Val Gly Gly Met Thr Arg
325 330 335
Ser Ala Arg Gly Thr Val Glu Ala Pro Gly Ser Asn Val Ala Ala Lys
340 345 350
Ala Gly Leu Asn Arg Ser Leu Leu Asp Ala Ala Leu Ala Glu Leu Arg
355 360 365
Arg Gln Leu Asp Tyr Lys Thr Ser Trp Tyr Gly Ser Arg Leu Ala Val
370 375 380
Val Asp Arg Trp Phe Pro Ser Ser Lys Thr Cys Ser Ala Cys Gly Trp
385 390 395 400
Arg His Pro Ser Gln Thr Leu Ala Asp Arg Val Phe Val Cys Ala Glu
405 410 415
Cys Gly Met Arg Val Asp Arg Asp Leu Asn Ala Ala Arg Asn Ile Ala
420 425 430
Ala His Ala Glu Pro Val Pro Gly Pro Gly Thr Val Ala Pro Gly Arg
435 440 445
Gly Glu Thr Ile Asn Ala Arg Gly Gly Arg Gly Ser Pro Ala Ala Leu
450 455 460
Arg Gly Ser Arg Arg Arg Pro Val Asn Arg Glu Asp Ala Gly Leu Ile
465 470 475 480
Gly Pro Ala Pro Pro Arg Arg Ser Asn Pro Pro Thr Ser Ser Ala Arg
485 490 495
Arg Pro Gln Asp Asp Asn Gly Ser Glu
500 505
<210> 63
<211> 566
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 63
Met Ala Thr Trp Gln Pro Gly Arg Pro Leu Pro Val Leu Val Arg Pro
1 5 10 15
Gly His Thr Leu Ser Ser Ala Phe Asp Phe Ala Gly Val Thr Phe Met
20 25 30
Pro Ala Leu Glu Val Thr Arg Ser Phe Arg Phe Ala Leu Asp Pro Thr
35 40 45
Glu Ala Gln Arg Glu Ser Phe Leu Lys Ser Ala Gly Ala Cys Arg Trp
50 55 60
Ala Tyr Asn Tyr Ala Leu Glu Lys Lys Thr Arg Ser His Gln Ala Trp
65 70 75 80
Val Ser Arg Arg Asp Ser Leu Ile Ala Lys Gly Met Ser Arg Pro Gln
85 90 95
Trp Lys Ala Gln Leu Arg Arg Glu Thr Ala Asp Leu Lys Ala Ser Cys
100 105 110
Ala Ala Trp Asp His His Arg Arg Ala Leu Ile Arg Met Ala Arg Ser
115 120 125
Ala Glu Pro Glu Val Ala Val Ala Pro Gln Glu Pro Ala Asp Leu Val
130 135 140
Ala Arg Leu Ala Arg Glu Arg Glu Glu Ala Arg Pro Gly Gly Ser Gly
145 150 155 160
Glu Glu Tyr Ala Arg Ala Leu Ala Arg Ala Arg Arg Met Val Ala Arg
165 170 175
His Lys Glu Glu Leu Phe Ala Arg Gly Asp Gln Ile Pro Asn Gln His
180 185 190
Asp Met Ala Ala Met Trp Arg Thr Glu Arg Asp Leu Pro Lys Glu Gln
195 200 205
Gly Gly Thr Pro Trp Trp Asn Glu Val Asn Val Tyr Cys Phe Thr Ser
210 215 220
Gly Phe Asp Arg Ala Gln Ala Ala Trp Asn Asn Trp Met Thr Ser Ala
225 230 235 240
Ser Gly Glu Arg Gly Gly Arg Arg Val Gly Tyr Pro Arg Phe Lys Lys
245 250 255
Lys Gly Gly Cys Leu Asp Ser Phe Thr Leu Tyr His Asp Val Gln Lys
260 265 270
Pro Arg Ile Arg Leu Asp Gly Tyr Arg His Leu Ile Ile Pro Thr Thr
275 280 285
Gly Arg Ile Arg Ile His Gly Ser Ala Lys Arg Leu Ala Arg Leu Val
290 295 300
Ala Leu Gly Glu Ala Arg Val Gln Ser Val Thr Val Thr Arg Arg Ala
305 310 315 320
His Arg Trp Tyr Ala Ser Val Ser Cys Lys Ile Thr Gln Arg Phe Pro
325 330 335
Ala Glu Pro Thr Leu Arg Gln Arg Ala Gly Gly Thr Val Gly Val Asp
340 345 350
Leu Gly Ser Arg Tyr Leu Ala Val Leu Ser Asn Gly Pro Gly Thr Ala
355 360 365
Gly Gly Arg Glu Ser Val Ile Pro His Pro Gln Phe Leu Leu Arg Asp
370 375 380
Leu Pro Arg Ile Asn Cys Ile Asn Arg Arg Met Arg Arg Met Gln Pro
385 390 395 400
Gly Ser Arg Glu Tyr Arg Arg Leu Ser Ala Arg Ala Ala Arg Arg His
405 410 415
His Leu Val Ala Leu Arg Arg Ala Gly Thr Leu His Asp Ile Thr Lys
420 425 430
Arg Leu Ser Arg Gly Tyr Ala Cys Ile Ala Val Glu Asp Phe Asp Leu
435 440 445
Val Ser Leu Val Thr Ser Ala Lys Gly Ser Arg Ser Arg Pro Gly Thr
450 455 460
Lys Val Lys Val Lys Ser Leu Phe Asn Arg Arg Leu Leu Asp Ala Ser
465 470 475 480
Ile Gly Glu Phe Arg Arg Gln Leu Ala Tyr Lys Cys Pro Gln Tyr Gly
485 490 495
Ser Arg Leu Ile Lys Ile Asp Arg Gly Glu Pro Val Ala Thr Thr Cys
500 505 510
Ser Arg Cys Gly Asp Arg Asn Pro Asn Ala Lys Pro Ser Gln Glu Lys
515 520 525
Phe Thr Cys Pro Ser Cys Arg Tyr Thr Ala Asp Arg His Ala Asn Ala
530 535 540
Ala Ala Cys Ile Tyr Met Ala Ala Leu Arg Lys Met Ser Ala Val Ala
545 550 555 560
Ser Gly Ser Gly Glu Thr
565
<210> 64
<211> 715
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 64
Met Glu Gln Ala Ser Leu Ile Met Cys His Ala Val Gly Phe Arg Trp
1 5 10 15
Ala Ala Glu Ala Asp Cys Val Phe Val Ile Cys Leu Thr Thr Gly Thr
20 25 30
Thr Arg Arg Ser Thr Arg Cys Asp Arg Thr Gln Ala Arg Val Phe Ala
35 40 45
Ser His Val Ser Lys Gly Ser Cys Leu Ala Arg Ala Glu Ala Ile Ala
50 55 60
Ser Val Arg Val Phe Phe Asp Cys Pro Thr Asn Thr Thr Val Trp Arg
65 70 75 80
Val Thr Gly Ser Leu Tyr Pro Met Ala Thr Cys Gly Pro Ala Gln Arg
85 90 95
Leu Gly Thr Gly Gly Thr Pro Arg Val Ala Ala Arg Ser Asn Pro Ala
100 105 110
Gly Pro Gly His Gly Pro Ala Ala Val Thr Met Pro Val Thr Glu Val
115 120 125
Leu Arg Ala Tyr Arg Phe Thr Leu Asp Pro Ser Asp Arg Glu Arg Ala
130 135 140
Ala Leu Ser Arg Tyr Ser Gly Ala Cys Arg Trp Ala Tyr Asn Tyr Ala
145 150 155 160
Val Ala Lys Lys Thr Gln Ala His Arg Ala Trp Ala Asp Arg Arg Thr
165 170 175
Ala Tyr Val Glu Ala Gly Leu Thr Glu Ala Glu Ala Lys Glu Arg Ile
180 185 190
Lys Ala Asp Gly Ala Glu Leu Thr Asp Arg Ile Lys Val Trp Asp His
195 200 205
His Arg Lys Ser Leu Thr Leu Thr Val Val Gly Lys Pro Pro Pro Ala
210 215 220
Ala Met Arg Ser Pro Ala Gly Gln Glu Ala Leu Val Arg Arg Leu Ala
225 230 235 240
Thr Ala Arg Ala Asp Ala Ala Gly Thr Ser Arg Glu Arg Ala Leu Leu
245 250 255
Ala Glu Gly Arg Ala Met Val Asn Ala Leu Lys Ala Gln Ala Phe Arg
260 265 270
Ala Gly Phe Arg Thr Pro Thr Ala Thr Asp Thr Ser Asn Leu Trp Arg
275 280 285
Met Glu Arg Asp Leu Pro Lys Glu Glu Gly Gly Ser Pro Trp Trp Ser
290 295 300
Glu Val Asn Val Tyr Cys Phe Thr Ser Gly Phe Asp Arg Ala Gln Val
305 310 315 320
Ala Trp Lys Tyr Trp Gln Asp Ser Leu Ser Gly Arg His Ala Gly Gln
325 330 335
Arg His Gly Tyr Pro Arg Phe Lys Lys Lys Gly His Ala Glu Ser Phe
340 345 350
Ser Leu Phe His Asp Val Met Arg Pro Ile Ile Arg Leu Glu Gly Tyr
355 360 365
Arg Arg Leu Val Met Pro Gly Leu Gly Ser Ile Arg Ile His Asp Ser
370 375 380
Gly Lys Arg Leu Ala Arg Leu Val Asp Arg Gly Gln Ala Val Ile Gln
385 390 395 400
Ser Val Thr Val Ser Arg Gly Gly His Arg Trp Tyr Ala Ser Val Leu
405 410 415
Ala Lys Val Gln Gln Asp Val Pro Val Leu Trp Glu His Val His Asp
420 425 430
Asp Gly Thr Arg Thr Pro Tyr Leu Ser Arg Ile Gln Ala Glu Lys Ala
435 440 445
Ser Glu Asn Gly Gly Arg Val Glu Gln Ile Gly Arg Pro Thr Ala Arg
450 455 460
Gln Arg Ala Gly Gly Leu Val Gly Val Asp Leu Gly Ser His His Leu
465 470 475 480
Ala Thr Leu Ser Ser Pro Leu Asp Pro Ala Asp Pro Ala Thr Ala Val
485 490 495
Val His Leu Pro Arg Leu Leu Ala Asp Ser Leu Ala Arg Leu Ser Lys
500 505 510
Ala Gln Arg Ala Met Ser Arg Cys Gln Gln Gly Ser Gly Arg Trp Arg
515 520 525
Lys Ala Thr Ala Arg Val Ser Arg Ala His Gln Gln Val Arg Val Arg
530 535 540
Arg Ala Ser Phe Leu His Gly Leu Ser Lys Lys Leu Ala Thr Gly Phe
545 550 555 560
Thr His Val Ala Ile Glu Asp Pro Asp Ile Thr Ala Leu Thr Thr Ser
565 570 575
Thr Glu Gly Thr Arg Asp Lys Pro Arg Lys Asn Ala Thr Ala Lys Ala
580 585 590
Arg Leu Asn Gln His Leu Leu Asp Ala Gly Leu Gly Ser Leu Arg Lys
595 600 605
Lys Leu Ala Tyr Lys Thr Ala Trp Tyr Gly Ser Gln Leu Val Val Leu
610 615 620
Asp His Gly Glu Pro Val Thr Ala Ser Cys Ala Thr Cys Lys Glu Arg
625 630 635 640
Asn Pro Ser Ser Asp Pro Ser Cys Ser Lys Val His Cys Pro Ser Cys
645 650 655
Gly Ala Val Val His Arg His Val Asn Ser Thr Ala Ser Ile Val Asp
660 665 670
Ala Ala His Arg Lys Leu Lys Thr Val Ala Ser Asp Arg Gly Glu Thr
675 680 685
Gln Asn Ala Arg Arg Ala Pro Gly Ser Pro Gly Ala Arg Lys Ala Pro
690 695 700
Gly Gln Gly Ala Ser Lys Arg Glu Asp Thr Gly
705 710 715
<210> 65
<211> 529
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 65
Met Gly Glu Gln Thr Thr Thr Arg Val Val Arg Leu Cys Leu Asp Thr
1 5 10 15
Arg Ala Leu Ser Glu Glu Gln Ala Val Leu Leu Ala Arg His Ala Gly
20 25 30
Thr Ala Arg Ala Val Trp Asn Trp Ala Leu Ala Ala Val Asn Ala His
35 40 45
Glu Asp Ala Val Arg Ala His Val Gln Asn Gln Ala Ala Gly Ala Ala
50 55 60
Ala Ser Ser Thr Ala Leu Met Asp Asp Ser Ala Trp Arg Arg Arg Ala
65 70 75 80
Tyr Ala Ala Ala Arg Ala Glu Leu Gly Arg Thr Pro Arg Ala Ile Ala
85 90 95
Leu Gly Arg Ala Phe Thr Ala Glu Thr Arg Asp Pro Glu Ser Arg Phe
100 105 110
Ala Trp Trp Gln Thr Glu Arg His Gly Val Asn Arg Phe Ala Val Ser
115 120 125
Ser Ala Leu Arg Ala Leu Asp Ala Ser Val Asp Arg Tyr His Gln Arg
130 135 140
Leu Thr Ala Pro Ala Arg Arg Thr Arg Arg Asp Gly Arg Pro Ala Gly
145 150 155 160
Trp Pro Arg Phe Lys Arg Lys Gly Arg Asp Arg Asp Ala Phe Ser Leu
165 170 175
Tyr Asn Leu Val Ile Ala Asp Gln Asp Pro Trp Arg Val Ile Asp Gly
180 185 190
Ala His Arg Ile Lys Val Pro Ser Leu Gly Ser Leu Arg Val His Glu
195 200 205
Asn Thr Arg Gln Leu Arg Arg Leu Ile His Arg Gly Gly Arg Pro Thr
210 215 220
Ala Ala His Phe Ser Arg His Gly Asp Arg Trp Tyr Val Ser Ile Asn
225 230 235 240
Val Ala Ile Ala Thr Thr Ser Leu Pro Ala Pro Val Thr Thr Arg Ala
245 250 255
Gln Arg Thr Ala Gly Val Val Gly Val Asp Leu Gly Val Lys His Leu
260 265 270
Ala Val Leu Ser Asp Gly Thr Val His Asp Asn Pro Arg Ala Ala Ala
275 280 285
Ala Ala Glu Arg Arg Leu Thr Arg Ala Gln Arg Ala Ala Ala Arg Arg
290 295 300
Ala Gly Pro Arg Arg Gly Val Gly Pro Ser Glu Gly Trp Lys Glu Ala
305 310 315 320
Gln Arg Ala Val Ala Arg Leu Arg His Arg Thr Ala Leu Arg Arg Gln
325 330 335
Gly Val Val His Glu Leu Thr Lys Ala Leu Ala Thr Gly Trp Ala Val
340 345 350
Val Ala Ile Glu Asp Leu Asn Val Arg Gly Met Thr Ala Ala Pro Leu
355 360 365
Ala Val Pro Asp Pro Asp Arg Pro Gly Ser Phe Leu Ser Asn Gly Ser
370 375 380
Ala Ala Lys Ser Gly Leu Asn Arg Ala Ile Leu Asp Val Gly Phe Gly
385 390 395 400
Glu Leu Arg Arg Gln Leu Thr Tyr Lys Ala Glu Arg Tyr Gly Ser Val
405 410 415
Val Val Ala Val Ala Arg Phe Ala Pro Thr Ser Lys Thr Cys Ser Gly
420 425 430
Cys Gly Ala Val Arg Ala Lys Leu Leu Leu Ser Glu Arg Thr Tyr Arg
435 440 445
Cys Asp Glu Cys Gly Leu Val Ile Asp Arg Asp Leu Asn Ala Ala Arg
450 455 460
Asn Ile Arg Asp Leu Ala Ser Ala Ile Val Ala Thr Ser Pro Ala Asp
465 470 475 480
Ala Gly Asp Ala Lys Arg Pro Asp Glu Arg Arg Arg Pro Arg Ala Thr
485 490 495
Gly Glu Gln Ala Gly Pro Ala Arg Pro Arg Thr Ala Gly Pro Pro Glu
500 505 510
Arg Val Thr Asp Ser Pro Ser Pro Gln Arg Thr Ala Pro Ser Leu Val
515 520 525
Ala
<210> 66
<211> 449
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 66
Met Gly Pro Leu Arg His Tyr Arg Glu Asn Pro Met Gln Gln Thr Val
1 5 10 15
Leu Arg Ala Tyr Arg Tyr Ala Leu Asp Pro Thr Pro Ala Gln Ala Asp
20 25 30
Met Leu Ala Ser His Ala Gly Ala Ala Arg Trp Ala Tyr Asn His Ala
35 40 45
Leu Ala Val Lys Ile Thr Ala His Lys Gln Trp Lys Gln Leu Val Ala
50 55 60
Glu Leu Val Glu Thr Gly Ile Pro Lys Ala Glu Ala Arg Lys Gln Ala
65 70 75 80
Asn Gln Gln Val Lys Ile Pro Thr Lys Pro Glu Ile Gln Lys Ala Leu
85 90 95
Asn Gln Val Lys Gly Asp Ser Arg Lys Gly Ile Asp Gly Leu Cys Pro
100 105 110
Trp His His Glu Val Ser Thr Tyr Ala Phe Gln Ser Ala Phe Glu Asp
115 120 125
Ala Asp Ala Ala Trp Lys Asn Trp Leu Ala Ser Thr Lys Gly Glu Arg
130 135 140
Lys Gly Pro Gln Val Gly Tyr Pro Lys Phe Lys Val Lys Gly Lys Ser
145 150 155 160
Arg Asp Ser Phe Arg Leu Tyr Gly Thr Ile Arg Leu Val Gly Tyr Arg
165 170 175
Arg Leu Phe Leu Pro Arg Ile Lys Glu Val Arg Leu His Gly Ser Gly
180 185 190
Lys Glu Leu Ala Arg Leu Val Glu Arg Gly Asp Ala Val Ile Gln Ser
195 200 205
Val Thr Val Ser Arg Gly Gly His Arg Trp Tyr Ala Ser Val Leu Ala
210 215 220
Lys Val Thr Met Val Ile Pro Asp Thr Pro Thr Lys Lys Gln Gln Thr
225 230 235 240
Arg Gly Met Val Gly Val Asp Leu Gly Val Lys Ala Leu Ala Ala Leu
245 250 255
Ser Lys Pro Leu Asn Pro Asp Asp Pro Ala Thr Ala Leu Val Pro Asn
260 265 270
Ala Arg His Leu Ala Ala Ala Glu Arg Arg Leu Lys Lys Ala Asp Arg
275 280 285
Ala Leu Ser Arg Lys Thr Lys Gly Ser Asn Arg Arg Ile Lys Ala Glu
290 295 300
Arg Arg Val Ala Lys Leu Arg His Gln Val Ala Val Arg Arg Ala Ser
305 310 315 320
Thr Ile His Gln Val Thr Lys Arg Leu Ala Thr Glu Phe Ala Thr Ile
325 330 335
Ala Ile Glu Asp Leu Asn Ile Ala Gly Met Thr Arg Ser Ala Lys Gly
340 345 350
Thr Ile Asp Ala Pro Gly Lys Asn Val Arg Ala Lys Ala Gly Leu Asn
355 360 365
Arg Asn Ile Leu Asp Ala Ala Leu Gly Glu Phe Arg Arg Gln Leu Glu
370 375 380
Tyr Lys Thr Ala Trp Tyr Gly Ser Gln Leu Val Val Val Asp Arg Trp
385 390 395 400
Leu Pro Ser Ser Lys Thr Cys Ser Ala Cys Gly Thr Val Lys Glu Thr
405 410 415
Leu Arg Leu Ser Asp Arg Val Phe Val Cys Asp Ala Cys Gly Met Arg
420 425 430
Leu Asp Arg Asp Leu Asn Ala Ala Arg Asn Ile Glu Ala Ala Ala Arg
435 440 445
Leu
<210> 67
<211> 577
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 67
Met Ile Gln Gln Ala Tyr Arg Phe Ala Leu Asp Pro Thr Pro Arg Gln
1 5 10 15
Glu Arg Met Leu Arg Ser His Ala Gly Ala Ala Arg Phe Ala Tyr Asn
20 25 30
Trp Gly Asn Ala Gln Ile Ile Ala Ala Leu Asp Ala Arg Glu Ala Glu
35 40 45
Lys Ala Ala Gly Thr Glu Pro Gly Thr Pro Val Pro Ser His Phe Asp
50 55 60
Leu Cys Lys Met Trp Thr Ala Trp Lys Asp Ala His Ala Ala Asp Pro
65 70 75 80
Glu Pro Pro Glu Gly Glu Arg Arg Thr Ser Thr Ala Trp Val Ser Glu
85 90 95
Asn Phe Val Gly Thr Tyr Gln Ala Ala Leu Arg Asp Ala Ala Lys Ala
100 105 110
Trp Ser Asp Tyr Phe Lys Ser Ala Arg Gly Glu Arg Lys Gly Arg Arg
115 120 125
Leu Gly Arg Pro Arg Phe Lys Lys Arg Gly Arg Ala Arg Asp Ser Phe
130 135 140
Gln Val His Gly Thr Thr Leu Arg Pro Val Ala Ala Ser Gly Asp Gly
145 150 155 160
Val Ala Phe Arg Ala Arg His Leu Ser Pro Gly Ser His Arg Asp Val
165 170 175
Ile Val Leu Pro Lys Ile Gly Pro Ile Leu Leu His Glu Ser Ala Arg
180 185 190
Lys Leu Arg Arg Arg Leu Gly Lys Gly Glu Val Pro Cys Pro Glu Cys
195 200 205
Cys Gln Ala Gly Val Val Pro Ala Thr Gly Ser Thr Arg Arg Lys Asp
210 215 220
Glu Val Pro Cys Pro Ala Gly Met His Ala Gly Ala Gly Cys Leu Val
225 230 235 240
Cys Asp Gly Gly Gly Arg Val Arg Pro Cys Pro Ala Cys Gly Gly Arg
245 250 255
Leu Ala Val Pro Ala Ala Arg Ile Ile Arg Gly Thr Val Ala Arg Asp
260 265 270
Ser Asp Gly Arg Trp Tyr Ile Ser Leu Thr Ala Glu Ile Ala Arg Glu
275 280 285
Val Arg Thr Arg Thr Val Arg Glu Val Gln Pro Ala Gly Thr Val Thr
290 295 300
Arg Thr Ala Val Pro Ala Pro Thr Ala Arg Gln Arg Ala Gly Gly Val
305 310 315 320
Val Gly Ala Asp Phe Gly Val Arg Asp Ile Ala Thr Leu Ser Asp Gly
325 330 335
Thr Ala Ile Ala Asn Pro Arg His Leu Glu Ala Ala Leu Gly Lys Leu
340 345 350
Ala Lys Ala Gln Gln Glu Val Ala Arg Cys Glu Lys Asp Ser Ala Gly
355 360 365
Arg Arg Gln Ala Ala Ala Arg Val Gly Arg Leu His Ala Arg Val Lys
370 375 380
His Leu Arg Leu Asp Ala Leu His Lys Ala Thr Ser Thr Leu Val Gln
385 390 395 400
Ser Arg Ala Val Ile Ala Val Glu Gly Phe Asp Val Gln Glu Thr Ala
405 410 415
Arg Tyr Arg Arg Gly Gly Lys Val Asp Arg Gly Lys Ala Glu Gly Arg
420 425 430
Glu Lys Val Pro Gly Lys Val Arg Gln Ala Arg Asn Arg Ala Leu Ala
435 440 445
Asp Thr Gly Ile Gly Ile Ala Arg Trp Gln Leu Gln Ser Lys Gly Ala
450 455 460
Trp Tyr Gly Cys Gln Val Leu Val Met Asp Lys His Ala Pro Thr Gly
465 470 475 480
Arg Thr Cys Ser Ala Cys Gly Thr Ala Lys Ala Lys Pro Val Pro Pro
485 490 495
Ala Glu Glu Met Phe Thr Cys Glu Ala Cys Gly Gly Val Met Pro Arg
500 505 510
Arg Val Asn Thr Ala Arg Ala Leu Ala Ser Arg Ala Arg Val Ser Val
515 520 525
Ser Val Ala Pro Ser Thr Gly Glu Thr Leu Asn Ala Arg Gly Gly Asp
530 535 540
Val Arg Pro Ala Ala Ala Arg Arg Gly Gly Gln Ser Pro Leu Lys Arg
545 550 555 560
Glu Ala Ser Thr Arg Pro Pro Gly Arg Gly Glu Pro Gly Ser Pro Gly
565 570 575
Gly
<210> 68
<211> 582
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 68
Met Lys Glu Thr Val Thr Arg Val Val Arg Tyr Arg Val Asp Val Asn
1 5 10 15
Ala Leu Thr Pro Thr Gln Ala Gln Leu Leu Asp Arg His Ala Gly Thr
20 25 30
Ala Arg Gln Ala Trp Asn Trp Ala Thr Ala Arg Trp Leu Asp Trp Gln
35 40 45
Ala Ser Ile Glu His Thr Val Trp Ala Gln Ala Ala His Ala Val Gly
50 55 60
Leu Asp His Ala Thr Thr Thr Thr Arg Leu Gly Asp Ala Leu Leu Ala
65 70 75 80
Ala Arg Ala Leu Leu Arg Gly His Glu Pro Glu Gln Arg Ala Trp Gln
85 90 95
Ser Thr Ala Tyr Thr Thr Ala Arg Ala Thr His Gly His His Ala Thr
100 105 110
Phe Glu Asp Ala Tyr Thr Leu Ala His Leu Tyr Arg Leu Thr Ala Ala
115 120 125
Gln Pro His Asp Pro Thr Ser Pro Asp Gly Leu Glu Arg Leu His Trp
130 135 140
Trp Ala Ala Gln Gly Thr Ser Ser Gly Leu Pro Arg Thr Gln Gln His
145 150 155 160
Gly Val Ser Thr Phe Ala Tyr Gln Thr Ala Leu Ala Asp Phe Ala Lys
165 170 175
Thr Ala Gln Ala Tyr Trp Ala Arg Pro Arg Tyr Ser Pro Arg Thr Gly
180 185 190
Arg Arg Leu Gly Ala Pro Arg Phe Lys Arg Arg Gly Glu His Asp Ala
195 200 205
Phe Ala Ile Met Asn Leu Thr Thr Thr Ala Ser Gly Pro Trp Lys Cys
210 215 220
Ile Leu Asp Gly His Arg Leu Leu Val Pro Asn Leu Gly Ser Leu Arg
225 230 235 240
Leu Thr Arg Asn Thr Arg Glu Leu Arg Arg Leu Ile Gln Arg Gly Gly
245 250 255
Arg Pro Thr Ser Val Arg Phe Thr Arg Thr Gly Gly Arg Trp His Leu
260 265 270
Ala Val Asn Val Ser Phe Glu Ala Thr Asn Pro Ala Val Leu Pro Pro
275 280 285
Pro Arg Pro Asn Arg Val Gln Ile Ala Gly Gly Ala Val Gly Val Asp
290 295 300
Leu Gly Ile Thr His Leu Ala Thr Leu Ser Thr Gly Thr His Ile Pro
305 310 315 320
Asn Pro Arg His Leu Thr Ala Ala Ala Ala Arg Leu Glu Asn Leu Gln
325 330 335
Gln Arg Arg Asp Arg Gln His Arg Ala Ala Ser Pro Thr Cys Phe Thr
340 345 350
Pro Asp Gly Thr His Lys Arg Gly Arg Cys Thr Trp Gly Arg Ala Asp
355 360 365
Gly Ala Pro Leu Ser Arg Arg Ala Arg Thr Thr Gln Ala Gln Ile Thr
370 375 380
Arg Leu His Ala Leu Leu Ala Thr Gln Arg Ala Ser Thr Leu His Gln
385 390 395 400
Leu Thr Lys His Leu Ala Thr Arg His Glu Arg Val Ala Ile Glu Asn
405 410 415
Leu Asn Pro Ala Ser Met Thr Arg Arg Pro Tyr Pro Lys Pro Asp Pro
420 425 430
Ala Gln Pro Gly Ala Tyr Leu Pro Asn Gln Arg Arg Ala Lys Ala Gly
435 440 445
Leu Asn Arg Ala Leu Leu Asp Ala Gly Phe Gly Glu Phe Arg Arg Gln
450 455 460
Leu Thr Tyr Lys Thr Thr Arg Tyr Gly Ser Thr Leu Thr Leu Val Pro
465 470 475 480
Pro Ala Gly Thr Ser Gln Thr Cys His His Cys Gly His Arg Ala Pro
485 490 495
Gln Asn Arg Glu Thr Gln Ala Asp Phe Val Cys Val Ser Cys Gly His
500 505 510
Val Ala Asn Ala Asp Ile Asn Ala Ala Cys Asn Ile Arg Asp His His
515 520 525
Val Ala Ala Pro Thr Glu Ser Gln His Pro Ala Gly Ala Gly Ala Ala
530 535 540
Thr His His Pro Pro Val Ala Val Ala Glu Gln Thr Pro Pro Gly Val
545 550 555 560
Asn Glu Thr Arg Lys Gly Ser Pro Val Gln His Thr Arg Thr Ala Asp
565 570 575
Pro Ala Arg Val Pro Ala
580
<210> 69
<211> 509
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 69
Met Lys Thr Glu Ile Leu Arg Ala Phe Arg Phe Thr Leu Ala Pro Thr
1 5 10 15
Pro Ala Gln Glu Gln Arg Leu Leu Arg Trp Cys Gly Asn Ser Arg Leu
20 25 30
Ala Phe Asn Tyr Ala Leu Ala Ala Lys Lys Ala Ala His Glu Asp Trp
35 40 45
Arg Thr Gln Val Val Ala Leu Val Asp Ala Gly Val Glu Glu Ala Thr
50 55 60
Ala Arg Lys Arg Val Lys Val Pro Thr Pro Thr Lys Pro Thr Val Tyr
65 70 75 80
Lys Arg Phe Val Ala Glu Arg Gly Asp Asp Arg Arg Gly Val Glu Gly
85 90 95
Ile Ala Pro Trp Trp His Glu Met Asn Thr Tyr Val Phe Gln Ser Ala
100 105 110
Phe Leu Asp Ala Asp Ala Ala Trp Lys Asn Trp Leu Asp Ser Phe Lys
115 120 125
Gly Lys Arg Ala Gly Arg Arg Val Gly Tyr Pro Arg Phe Lys Lys Arg
130 135 140
Gly Leu Ala Arg Asp Ser Phe Arg Leu His His Asp Val Lys Lys Pro
145 150 155 160
Gly Ile Arg Leu Thr Thr Tyr Arg Arg Leu Arg Leu Pro Ala Phe Gly
165 170 175
Glu Ile Arg Leu His Asp Asn Ala Arg Arg Leu Gly Arg Leu Ile Ser
180 185 190
Arg Glu Glu Ala Arg Ile Gln Ser Val Thr Val Ser Arg Ser Gly His
195 200 205
Arg Trp Tyr Ala Ser Val Leu Cys Lys Val Gln Val Asp Leu Pro Glu
210 215 220
Lys Pro Thr Arg Ala Gln Gln Thr Ala Gly Thr Val Gly Val Asp Leu
225 230 235 240
Gly Val Lys Met Leu Ala Ala Leu Ser Arg Pro Leu Asp Pro Lys Ser
245 250 255
Pro Asp Arg Glu Gly His Phe Val Thr Asn Pro Arg His Leu Ala Arg
260 265 270
Val Ala Lys Arg Leu Arg Gln Ala Gln Arg Ala Leu Ser Arg Lys Gln
275 280 285
Lys Gly Ser Ala Arg Arg Asp Lys Ala Arg Arg Arg Val Ala Arg Leu
290 295 300
His His Glu Val Thr Val Arg Arg Arg Ala Ala Leu His Gln Val Thr
305 310 315 320
Lys Lys Leu Ala Thr Arg Tyr Ala Thr Ile His Ile Glu Asp Leu His
325 330 335
Val Ala Gly Met Thr Ala Thr Ala Arg Gly Thr Leu Lys Arg Pro Gly
340 345 350
Arg Ser Val Arg Gln Lys Ala Gly Leu Asn Arg Ala Ile Leu Asp Thr
355 360 365
Ala Met Ala Glu Thr Arg Arg Gln Leu Thr Tyr Lys Thr Ser Trp Tyr
370 375 380
Gly Ser Gln Leu Ala Val Leu Asp Arg Trp Trp Pro Ser Ser Lys Thr
385 390 395 400
Cys Ser Asp Cys Gly Trp Arg Asn Pro Ser Leu Thr Leu Asn Glu Arg
405 410 415
Val Phe His Cys Ala His Cys Gly Leu Val Leu Asp Arg Asp Leu Asn
420 425 430
Ala Ala Arg Asn Ile Glu Arg His Thr Ala Ala Arg Pro Thr Pro Gln
435 440 445
Gln Val Ala Ser Gly Lys Gly Glu Thr Gln Asn Ala Arg Gly Glu Ser
450 455 460
Val Arg Leu Pro Arg Pro Arg Ala Glu Lys Gln Asp Pro Val Lys Arg
465 470 475 480
Glu Asp Ala Arg Pro Ser Pro Ala Pro Pro Arg Arg Ser Asn Pro Pro
485 490 495
Thr Ser Pro Pro Thr Tyr Asn Gly Gln Ala Lys Ser Ser
500 505
<210> 70
<211> 509
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 70
Met Lys Thr Glu Val Leu Arg Ala Tyr Arg Phe Thr Leu Ala Pro Ser
1 5 10 15
Pro Ala Gln Glu Gln Lys Leu Leu Arg Trp Cys Gly Asn Ala Arg Leu
20 25 30
Ala Phe Asn Tyr Ala Leu Ala Ala Lys Lys Gln Ala His Glu Gln Trp
35 40 45
Arg Ala Gln Val Asp Glu Leu Val Ala Gly Gly Met Pro Glu Ala Gln
50 55 60
Ala Arg Lys Gln Thr Lys Val Pro Ile Pro Ser Lys Pro Ala Val Gln
65 70 75 80
Lys Ala Phe Asn Ala Glu Arg Gly Asp Thr Arg Ala Asp Val Glu Gly
85 90 95
Val Cys Pro Trp Trp His Glu Val Asn Thr Tyr Val Phe Gln Ser Ala
100 105 110
Phe Ile Asp Ala Asp Arg Ala Trp Lys Asn Trp Leu Asp Ser Phe Ala
115 120 125
Gly Lys Arg Ala Gly Arg Arg Val Gly Tyr Pro Arg Phe Lys Lys Arg
130 135 140
Gly Arg Ala Arg Asp Ser Phe Arg Leu His His Asp Val Lys Lys Pro
145 150 155 160
Gly Ile Arg Leu Ala Thr His Arg Arg Leu Arg Leu Gly Ser Phe Gly
165 170 175
Glu Val Arg Leu His Asp Ser Gly Lys Arg Leu Phe Arg Gln Ile Asp
180 185 190
Arg Gly Glu Ala Val Val Gln Ser Val Thr Val Ser Arg Gly Gly His
195 200 205
Arg Trp Tyr Ala Ser Val Leu Cys Lys Val Thr Thr Asp Val Pro Asp
210 215 220
His Pro Thr Arg Arg Gln Ala Glu Arg Gly Lys Val Gly Val Asp Leu
225 230 235 240
Gly Val Lys Val Leu Ala Ala Leu Ser Lys Pro Leu Pro Thr Ser Gly
245 250 255
Ala Val Lys Val Ser Gly Gly Gly Leu Leu Val Asp Asn Pro Arg Pro
260 265 270
Leu Arg Ala Ala Ala Gln Arg Leu Val Lys Ala Gln Arg Ala Leu Ser
275 280 285
Arg Thr Gln Lys Gly Ser Ala Arg Arg Glu Lys Ala Gln Arg Arg Val
290 295 300
Gly Arg Leu His His Gln Val Ala Val Arg Arg Asp Ala Ala Leu His
305 310 315 320
Gln Val Thr Lys Ala Leu Ala Thr Arg Phe Ala Val Val Ala Val Glu
325 330 335
Asn Leu Asn Val Ala Gly Met Thr Arg Ser Ala Arg Gly Ser Val Glu
340 345 350
Ala Pro Gly Arg Arg Val Arg Gln Lys Ala Gly Leu Asn Arg Ala Ile
355 360 365
Leu Asp Ser Ala Pro Gly Glu Leu Arg Arg Gln Leu Ala Tyr Lys Thr
370 375 380
Ser Trp Tyr Gly Ser Gln Leu Ala Val Leu Asp Arg Trp Trp Pro Ser
385 390 395 400
Ser Lys Thr Cys Ser Asn Cys Gly Trp Gln His Pro Ser Leu Thr Leu
405 410 415
Asn Asp Arg Thr Phe His Cys Thr Thr Cys Gly Leu Thr Met Asp Arg
420 425 430
Asp Leu Asn Ala Ala Leu Asn Ile Glu Arg His Thr Glu Thr Pro Asn
435 440 445
Val Ala Ser Asp Thr Gly Glu Thr Lys Asn Ala Arg Arg Ala Ser Ile
450 455 460
Ser Leu Pro Gly Leu Arg Ala Glu Lys Gln Asp Ala Thr Lys Arg Glu
465 470 475 480
Asp Ala Asp Pro Thr Gly Pro Ala Pro Pro Arg Arg Ser Asn Pro Pro
485 490 495
Thr Leu Pro Pro Gln Arg His Glu Gln Ala Lys Leu Phe
500 505
<210> 71
<211> 760
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 71
Met Met Ala Val Leu Val Val Gly Ala Leu Cys Cys Ala Asp His Met
1 5 10 15
Arg Pro Ser Ser Asp Arg Val Gly Arg Trp Arg Thr Val Arg Leu Thr
20 25 30
Leu Asp Pro Thr Arg Arg Gln Ile Lys Val Met Glu Met Tyr Cys Leu
35 40 45
Leu Asp Arg Arg Ala Tyr Glu Arg Ala Leu Pro Asp Gly Pro Cys Pro
50 55 60
Asn Gly Asp Ser Ser His Ala Ser Val Ser Arg Gln Pro Asp Val Ala
65 70 75 80
Ala Val Leu Ala Ser Ile Glu Pro Pro Ser Thr Pro Gly Asp Leu Glu
85 90 95
Thr Lys Trp Ala Val Val Ser Ser Ala His His Tyr Ala Ala Ala Val
100 105 110
Ser Gly Arg Arg Ala Leu Arg Val Pro Pro Arg Ser Ala Arg Arg Pro
115 120 125
Ala Pro Arg Lys Arg Pro Gln Thr Ser Thr Arg Thr Met Phe Gly Leu
130 135 140
Tyr Glu Arg Gly Gly Arg Pro His Phe Glu Ile Gln Ser Tyr Arg Arg
145 150 155 160
Val Arg Ile Gly Pro Leu Ala Ser Ile Arg Ile His Asn Ser Ala Lys
165 170 175
Gly Leu Ala Ala Glu Leu Lys Asp Pro Ala Val Arg Leu Val Met Thr
180 185 190
Thr Val His Arg Lys Ala His Arg Trp Tyr Ala Ser Ile Leu Ile Glu
195 200 205
Asp Ser Arg Pro Leu Pro Ala Pro Thr Ala Arg Gln Lys Arg Asn Gly
210 215 220
Pro Val Gly Val Asp Leu Gly Val Leu His Leu Ala Thr Leu Ser Thr
225 230 235 240
Gly Glu Thr Ile Pro Asn Pro Lys Val Phe Glu Arg Gly Arg Lys Gln
245 250 255
Val Ala Lys Ala Ser Gln Ala Val Ala Arg Ser Ala Glu Gly Ser Arg
260 265 270
Arg His Gln Lys Ser Val Glu Arg Leu His Arg Arg His His Glu Ile
275 280 285
Ala Glu His Arg Lys Thr Val Leu His Thr Ile Thr Arg Arg Leu Ala
290 295 300
Thr Ser Trp Ser His Ile Ala Ile Glu Asp Leu Asn Val Arg Gly Met
305 310 315 320
Thr Thr Ser Ala Arg Gly Thr Ile Glu Asn Pro Gly Arg Asn Ile Lys
325 330 335
Ala Lys Ser Gly Leu Asn Arg Ala Leu Arg Asp Val Ser Pro Trp Lys
340 345 350
Phe Arg Glu Gln Ile Ser Tyr Lys Ser Gly Trp Tyr Gly Ser Glu Leu
355 360 365
Val Ile Cys Ser Arg Trp Phe Pro Ser Ser Lys Arg Cys Ser Gln Cys
370 375 380
Gly Thr Ile Arg Arg Ala Leu Pro Leu Thr Gln Arg Ile Phe Arg Cys
385 390 395 400
Ala Thr Cys Gln Leu Thr Ile Asp Arg Asp Leu Asn Ala Ala Val Asn
405 410 415
Leu Ala Ser Arg Val Gly Val Ala Trp Gly Thr Trp Glu Thr Glu Asn
420 425 430
Ala Arg Lys Glu Ser Ile Ile Thr Asn Thr Ala Gly Val Ser His Asp
435 440 445
Ala Ala Ser Thr Gly Arg Pro Arg Ala Asp Ser Ser Ala Thr Leu Asp
450 455 460
Glu Gln Ser Ser Ser His Pro Thr Arg Ser Asn Arg Gln Gly Gly Pro
465 470 475 480
Lys Thr Leu Arg Gly Pro Gly Pro Leu Thr Ser Arg Lys Gln Pro Asp
485 490 495
Trp Thr Arg Tyr His Thr Gln Trp Leu Leu Asn Cys Leu Ala Leu Ala
500 505 510
Ala Gln Gln Pro Pro Thr Thr Arg Ala Arg Trp Glu Ala Val Thr Gln
515 520 525
Ser Leu Ala Glu Asn Ser Gln Arg Ser Gly Asn Val Glu His Gly Met
530 535 540
Pro Val Val Ala Val Gly Ser Arg Ala Leu Ala Ile Gly Ala Gln Tyr
545 550 555 560
Leu Cys Glu Ser Val Ile Gln Arg Cys Leu Leu Gln Leu Ser Ala Ile
565 570 575
Ser Pro Asp Pro Pro Ile Ala Leu Val Arg Arg Gly Arg Gly Leu Gly
580 585 590
Ala Asp Arg Trp Ala Leu Leu Pro Val Thr Ser Pro Thr Glu Ile Ala
595 600 605
Thr Gly Ile Lys Pro Met His Pro Ala Trp His Ile Val Gly Pro Ala
610 615 620
His Met Pro Thr Tyr Glu Leu Val Gln Lys His Gly Pro Thr Ser Pro
625 630 635 640
Val Glu Ile Ile Ser Ala Ala Thr His Ile Gly Lys Ser Ala Ala Tyr
645 650 655
Asn Ser Phe Asn Val Leu Gln Gln Trp Gly Leu Ile Thr Arg Arg Pro
660 665 670
Arg Tyr Ile Ala Leu Gly Pro Thr Thr Leu Asp Asp Ile Leu Asp Gln
675 680 685
His Asp His Ala Ala Ala Lys Asp Ala Met Leu Asp Arg His Arg Arg
690 695 700
Gln Arg Ala Gly Trp Arg Lys Thr Leu Asn Leu Ile Glu Tyr Ala Arg
705 710 715 720
Thr Ser His Asp Ile Ala Glu Leu Ala Pro Asp Ser Pro Gln Leu Thr
725 730 735
Ala Gly Asp Ser Glu Asp Glu Gln Gln Tyr Arg Ile Ala Val Met Ala
740 745 750
Gly Pro Pro Pro Pro Glu Ala Pro
755 760
<210> 72
<211> 494
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 72
Met Asn Glu Arg Arg Leu Arg Arg Leu Arg Thr Tyr Arg Phe Thr Leu
1 5 10 15
Asp Pro Thr Arg Ala Gln Val Asp Val Leu Val Gln His Ala Gly Ala
20 25 30
Ala Arg Trp Ala Tyr Asn His Ala Leu Ala Ala Lys Phe Asp Ala Leu
35 40 45
Arg Arg Arg Gln Leu Gln Ile Asp Glu Leu Val Thr Leu Gly Leu Thr
50 55 60
Glu Thr Gln Ala Arg Arg Glu Ala Thr Val Lys Val Pro Ser Lys Pro
65 70 75 80
Gln Ile Gln Arg Arg Trp Asn Gln Leu Lys Gly Asp Ala Thr Arg Asp
85 90 95
Ile Asp Gly Ile Cys Pro Trp Trp Arg Ala Val Ser Thr Tyr Ala Phe
100 105 110
Gln Ser Ala Phe Leu Asp Ala Asp Gln Ala Trp Lys Asn Trp Met Asp
115 120 125
Ser Leu Ser Gly Lys Arg Ala Gly Arg Arg Met Gly Leu Pro Lys Phe
130 135 140
Lys Lys Lys Gly Arg Ser Arg Asp Ser Phe Arg Leu His His Asp Val
145 150 155 160
Lys Asn Pro Thr Ile Arg Leu Asp Ser Tyr Arg Arg Leu Arg Met Pro
165 170 175
Arg Leu Gly Ser Ile Arg Leu His Asp Ser Gly Lys Arg Leu Ala Arg
180 185 190
Ala Leu Asp Arg Gly Gly Arg Val Gln Ser Val Thr Val Ala Arg Gly
195 200 205
Gly His Arg Trp Tyr Ala Ser Val Leu Val Asp Glu Pro Asp Ile Thr
210 215 220
Pro Gly Arg Glu Thr Gln His Gly Pro Ser Pro Ala Gln Arg Ala Ala
225 230 235 240
Gly Gly Val Gly Val Asp Ile Gly Val Leu His Leu Ala Ala Leu Ser
245 250 255
Thr Gly Glu Val Val Glu Asn Pro Arg His Val Arg Arg Ala His Ala
260 265 270
Arg Leu Val Lys Ala Gln Arg Ala Leu Ser Arg Thr Val Lys Gly Ser
275 280 285
Arg Arg Arg Arg Arg Ala Ala Ala Arg Val Ala Arg Ile His His Gln
290 295 300
Leu Ala Glu Arg Arg Ala Thr His Leu His Gly Ile Thr Lys Arg Leu
305 310 315 320
Ala Thr Arg His Ser Leu Val Ala Ile Glu Asp Leu Asn Val Ala Gly
325 330 335
Met Thr Arg Ser Ala Arg Gly Thr Val Ala Glu Pro Gly Arg Asn Val
340 345 350
Arg Ala Lys Ala Arg Leu Asn Arg Ala Ile Leu Asp Val Ala Pro Gly
355 360 365
Glu Leu Arg Arg Gln Leu Glu Tyr Lys Thr Ser Trp Tyr Gly Ser Thr
370 375 380
Leu Ala Val Cys Asp Arg Trp Ala Pro Thr Ser Lys Thr Cys Ser Asn
385 390 395 400
Cys Gly Thr Val Lys Thr Lys Leu Ser Leu Ser Thr Arg Val Phe Gln
405 410 415
Cys Asp Asp Cys Gly Met Val Leu Asp Arg Asp Val Asn Ala Ala Arg
420 425 430
Asn Val Leu Ala Val Ala Val Pro His Val Ala Ser Gly Gly Gly Glu
435 440 445
Thr Leu Asn Ala Arg Gly Glu Arg Val Ser Pro Ala Pro Arg Ile Ala
450 455 460
Ala Met Ala Val Leu Ser Asp Ala Gly Arg Pro Gly Arg Ser Ala Arg
465 470 475 480
Ser Pro Arg Gly Ser Asp Pro Pro Ser Ile Pro Asn Pro Gln
485 490
<210> 73
<211> 545
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 73
Met Asn Arg Arg Glu Gln Arg Ile Gly Phe Ala Ile His Leu Phe Ala
1 5 10 15
Ala Phe Ile Ser Gly Gly Cys Asp Gly Ala Gly Met Asp Glu Asn Thr
20 25 30
Thr Thr Val Val Val Arg Glu Thr Leu Asp Pro Thr Ala Asp Gln Arg
35 40 45
Ala Ile Leu Gln Arg Tyr Ala Asp Ala Ser Arg Cys Ser Phe Asn Tyr
50 55 60
Ala Leu Gly Leu Lys His Gly Thr Gln Gln Leu Trp Ala His Gly Arg
65 70 75 80
Asp Arg Leu Ile Ala Gln Gly Gln Thr Pro Ala Glu Ala Ala Arg Asn
85 90 95
Ala Pro Lys Val Asp Val Pro Ser Gln Phe Ala Val Gln Lys Ile Phe
100 105 110
Leu Ala Gln Arg Asp Gln Pro Leu Pro Gly Pro Leu Leu Pro Gly Gln
115 120 125
Glu Pro Arg Leu Leu Phe Pro Trp Trp Lys Gly Val Asn Ala Ile Val
130 135 140
Cys Gln Gln Ala Phe Arg Asp Ala Asp Thr Ala Phe Asn Asn Trp Lys
145 150 155 160
Ser Ser Gly Arg Arg Lys Gly Ala Pro Val Gly Tyr Pro Arg Phe Lys
165 170 175
Arg Arg Gly Arg Cys Arg Asp Ser Phe Arg Met Phe Ala Val Arg Leu
180 185 190
Val Glu Gln Asp Leu Arg His Val Arg Ile Gly Gly Gly Gly Gly Gln
195 200 205
Pro Ala Phe Ser Val Arg Leu His Arg Pro Ala Arg Arg Leu Ala Arg
210 215 220
Leu Leu Ala Arg Gly Gly Val Ala Lys Ser Val Thr Ile Ser Arg Glu
225 230 235 240
Gly His Arg Trp Val Ala Ala Phe Asn Val Arg Val Pro Val Gly Pro
245 250 255
Val Pro Arg Pro Ser Arg Arg Gln Arg Glu Ala Gly Thr Val Gly Val
260 265 270
Asp Leu Gly Val Lys Val Phe Ala Ala Thr Ser Asp Pro Val Ile Ile
275 280 285
Asn Asp His Lys Ile Gln Leu Phe Glu Asn Pro Arg His Leu Glu Asn
290 295 300
Thr Arg Arg Gln Leu Arg Lys Trp Gln Arg Arg Met Ala Arg Arg His
305 310 315 320
Val Arg Gly Leu Arg Ser His Glu Gln Ser Gln Gly Trp Lys Glu Ala
325 330 335
Arg Asp Gln Val Ala Arg Leu His Ala Leu Val Ala Ala Arg Arg Ala
340 345 350
Ser Ser Gln His Leu Val Thr Lys Arg Leu Val Thr Gln Tyr Ala His
355 360 365
Val Ala Leu Glu Asp Leu Arg Val Lys Asn Met Thr Ala Ser Ala Arg
370 375 380
Gly Thr Ala Glu Ser Pro Gly Arg Asn Val Arg Ala Lys Ala Gly Leu
385 390 395 400
Asn Arg Ala Ile Leu Asp Val Gly Phe Gly Glu Ile Arg Arg Gln Ile
405 410 415
Glu Tyr Lys Ala Leu Leu Tyr Gly Ala Gln Val Thr Val Val Asp Pro
420 425 430
Ala Tyr Thr Ser Gln Thr Cys Asn Arg Cys Gly His Val Asp Ala Lys
435 440 445
Ser Arg Arg Thr Arg Asp Gln Phe Thr Cys Thr Arg Cys Gly His Ala
450 455 460
Thr His Ala Asp Ile Gly Ala Ala Ile Asn Ile Lys Ala Arg Ala Glu
465 470 475 480
Leu Thr Ser Glu Ser Ala Ala Pro Gly Leu Ala Asn His Asp Gln Gly
485 490 495
Val His Cys Gly Ala Pro Ala Asn Gln Thr Arg Pro Lys Ala Arg Arg
500 505 510
Tyr Ala Pro Pro Ala Lys His Gly Met Pro Tyr Ser Gly Glu Val Ser
515 520 525
Gly Lys Val Pro Lys Gly Arg Ala Ser His Ser Pro Val Ser Gln Pro
530 535 540
Gly
545
<210> 74
<211> 433
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 74
Met Pro Ala Asp Lys Glu Pro Lys Asp Ile Leu Ile Phe Arg Thr Arg
1 5 10 15
Leu Lys Pro Asn Lys Thr Gln Glu Glu Ala Leu Trp Arg Asn Cys Ala
20 25 30
Gly Tyr Arg Trp Val Tyr Asn Tyr Met Leu Ala Leu Gln Lys Glu Arg
35 40 45
Tyr Glu Lys Trp Arg Glu Ala Lys Glu Ala Trp Glu Lys Glu Asn Pro
50 55 60
Asp Gly Gly Lys Phe Lys Ala Pro Asp Asp Leu Lys Pro His Ser Ala
65 70 75 80
Tyr Asp Leu Lys Lys Leu Leu Pro Val Leu Lys Ser Asp Glu Pro Thr
85 90 95
Ala Trp Leu Gly Glu Leu Asp Ser Gln Val Leu Gln Asp Ala Val Leu
100 105 110
Asn Phe Gly Lys Thr Trp Gln Arg Phe Leu Gly Arg Ser Lys Thr Gly
115 120 125
Ser Gly Phe Pro Lys Phe Arg Asn Arg Asp Lys Asn Asn Tyr Ile Ser
130 135 140
Arg Ser Asn Gly Gly Lys Gln Gln Trp Val Asn Glu Asn His Ile Asn
145 150 155 160
Leu Pro Lys Ile Gly Asn Val Lys Met Tyr Glu Arg Pro His Ile Lys
165 170 175
Asn Arg Pro Asp Ala His Leu Leu Asp Leu Thr Arg Pro Ser Gly Tyr
180 185 190
Ile Ile Ser Arg Lys Ala Asn His Trp Trp Ile Ser Leu Arg Tyr Gln
195 200 205
Ala Pro His Ile Glu Pro Lys Lys Arg Lys Gly Glu Glu Val Gly Ile
210 215 220
Asp Leu Gly Val Ala Arg Trp Ala Thr Leu Ser Asp Gly Arg His Ile
225 230 235 240
Gly Arg Asp Asp Arg Ile Glu Ala Lys Leu Lys Arg Leu Leu Glu Arg
245 250 255
Lys Asp Phe Tyr Tyr Arg Gln Leu Ala Arg Arg Pro Lys Gln Lys Ser
260 265 270
Glu Glu Glu Gly Arg Thr Tyr Ser Gly Ser Asn Arg Glu Lys Thr Lys
275 280 285
Lys Lys Leu Ala Lys Thr Trp Ala Arg Ile Ala Glu Ile Lys Arg Asn
290 295 300
Tyr Ser His Cys Ala Ser Lys Glu Leu Ser Gln Gln Phe Ala Lys Ile
305 310 315 320
Ala Leu Glu Glu Leu Lys Ile Gln Asn Met Thr Arg Ser Ala Lys Gly
325 330 335
Thr Val Glu Glu Pro Gly Arg Asn Val Arg Gln Lys Ser Gly Leu Asn
340 345 350
Arg Ser Ile Asn Gln Gln Asn Trp Tyr Gln Phe Arg Ser Met Leu Glu
355 360 365
Tyr Lys Met Glu Arg Asn Gly Gly Glu Val Val Thr Val Asn Pro Lys
370 375 380
Asn Thr Ser Ile Thr Cys Ser Lys Cys Gly His Ala Asp Lys Ala Asn
385 390 395 400
Arg Pro Ser Gln Ser Glu Phe Lys Cys Gln Lys Cys Gly Phe Glu Glu
405 410 415
Asn Ala Asp Leu Asn Ala Ala Ile Asn Ile Cys His Ile Ala Phe Gly
420 425 430
Gly
<210> 75
<211> 681
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 75
Met Pro His Glu Thr Arg Leu Val Gln Arg Ala Tyr Lys Phe Ala Leu
1 5 10 15
Asp Pro Thr Pro Glu Gln Ala Ala Gln Phe Ala Ser His Ala Gly Gly
20 25 30
Ala Arg Phe Ala Phe Asn Trp Gly Ile Ala Thr Tyr Ala Ala Ala Leu
35 40 45
Asn Ala Arg Gln Ala Gln Lys Asp Ala Gly Gln Arg Ile Thr Ile Asp
50 55 60
Leu Pro Asp His Phe Thr Leu Cys Lys Leu Trp Thr Ala Tyr Lys Asp
65 70 75 80
Thr Tyr Arg Asp Thr Pro Asp Pro Asp Gly Arg Thr Thr His Trp Val
85 90 95
Glu Asn Asn Phe Val Gly Thr Tyr Gln Ala Ala Leu Arg Asp Ala His
100 105 110
Gly Ala Trp Gln Lys Met Trp Asp Ser Arg Ala Gly Arg Arg Lys Gly
115 120 125
Arg Ala Val Gly Arg Pro Arg Phe Lys Lys Lys Gly Arg Ser Gln Asp
130 135 140
Ser Phe Gln Met His Gly Asp Thr Leu Arg Ile Asp Thr Gly Ser Ala
145 150 155 160
Val Arg Pro Val Gly Ala Pro Arg Val Lys Arg Arg Pro Ala Glu Val
165 170 175
Gly Glu Pro Trp Gly His Ala Leu Arg Arg Leu Thr Asp Ser Ala Arg
180 185 190
Lys Phe Tyr His Glu Arg Arg Asp Arg Lys Gly Phe Gly Cys Ala Thr
195 200 205
Pro Val Gly Val Pro Asn Lys Arg Gly Trp Val Val Asp His Pro Cys
210 215 220
Pro Gly Ala Val Thr His Val Leu Thr Tyr Arg Ala Lys Ala Val Arg
225 230 235 240
Arg Ser Asp Pro Thr Val Val Asp Arg Thr Val Ala His Lys Arg Asn
245 250 255
Leu Cys Asp Glu His Ala Ala Gly Val Ala Gln Glu Arg Gly Ile Asp
260 265 270
Leu Pro Ala Glu Ala Lys Lys Ala Gly Val Thr Gly Tyr Gly Pro Ala
275 280 285
Ala Ser Thr Ser Gly Ser Arg Tyr Met Ser Leu Pro Lys Val Gly Gln
290 295 300
Val Lys Ile Pro Gly Lys Leu Arg Ile Gly Gln Arg Arg Arg Val Thr
305 310 315 320
Pro Thr Pro Gly Glu Thr Gln Trp Val Asp Arg Ser Ala Arg Val Ala
325 330 335
Arg Lys Leu Ala Arg Leu Leu Arg Arg Ser Glu Thr Ala Gly Pro Val
340 345 350
Val Cys Pro Thr Cys Asn Gly Thr Gly Glu Val Gln Val Thr Thr Lys
355 360 365
Ala Gly Glu Pro Lys Thr Ser Lys Cys Gly Val Tyr Gly Pro His Val
370 375 380
Ala Pro Asp Asp Lys Arg Pro Gly Cys Arg Gly Ser Gly Lys Val Glu
385 390 395 400
Gln Ala Arg Ile Val Arg Ala Thr Leu Ser Arg Gly Ala Ser Gly Thr
405 410 415
Trp Trp Cys Ser Val Thr Ala Glu Val Val Met Gln Val Arg Thr Ala
420 425 430
Pro Ser Lys Arg Gln Thr Arg Asn Gly Val Val Gly Leu Asp Leu Gly
435 440 445
Val Arg His Leu Ala Val Ala Ser Thr Gly Asp Thr Tyr Thr Asn Ser
450 455 460
Arg Phe Leu Asp Ala Asn Leu Ala Asp Leu Arg Arg Leu Gln Lys Ala
465 470 475 480
Leu Ser Arg Ala Glu Lys Asp Ser Lys Arg Arg Glu Lys Ala Lys Arg
485 490 495
Arg Val Gly Ala Leu His Glu Gln Val Ala Leu Met Arg Lys Asp Ala
500 505 510
Cys Asp Arg Ile Thr Ser Glu Met Ala Asn Thr Tyr Ala Lys Val Gly
515 520 525
Val Glu Gly Trp Asp Ala Gln Arg Leu Ala Glu Lys Gly Ser Glu Gly
530 535 540
Val Pro Val Arg Leu Arg Lys Ala Arg Asn Arg Ala Leu Ala Asp Ala
545 550 555 560
Ala Pro Gly Met Phe Arg Trp Gln Leu Thr Tyr Lys Thr Ser Trp Cys
565 570 575
Gly Ser Gln Tyr Asn Gln Gly Glu Pro His Trp Glu Thr Gly Arg Ile
580 585 590
Cys Ser Val Cys Gly Arg Val Arg Thr Lys Pro Val Pro Leu Thr Glu
595 600 605
Glu Gln Phe Arg Cys Asp Gly Gly His Val Val Asp Arg Arg Val Asn
610 615 620
Ser Ala Arg Val Met Glu Lys Val Thr Ala Gly Leu Ile Ala Pro Pro
625 630 635 640
Gly Val Val Asp Glu Pro Arg Gly Gly Asp Val Arg Pro Val Thr Pro
645 650 655
Arg Arg Asp Gly Arg Ser Pro Ala Lys Arg Ala Ala Arg Thr Arg Lys
660 665 670
Arg Gly Lys Ala Gly Thr Pro Asp Pro
675 680
<210> 76
<211> 528
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 76
Met Pro His Ile Thr Ile Lys Arg Ala Tyr Arg Val Ala Leu Ser Pro
1 5 10 15
Thr Val Ala Gln Gln Cys Arg Leu Ala Arg His Glu Gly Ala Asn Arg
20 25 30
Ala Ala Tyr Asn Phe His Leu Ala Ala Lys Gln Gln Ala His Gln Gln
35 40 45
Trp Lys Ala Ala Val Glu Thr Val Trp Gln Asp Gln Tyr Ala His Leu
50 55 60
Ala Pro Asp Glu Gly Tyr Arg Val Ala Lys Lys Ala Val Asn Gln Ala
65 70 75 80
Lys Ala Val Arg Phe Pro Thr Tyr Met Asp Ser Met Lys Ala Leu Arg
85 90 95
Ala Asp Lys Lys Tyr Ala Trp His Ala Glu Val Asn Gly Tyr Ala Leu
100 105 110
Ser Ser Gly Met Gln Ala Val Asp Arg Ala Trp Lys Asn Trp Val Ser
115 120 125
Ser Tyr Thr Gly Glu Arg Ala Gly Gln Ala Val Gly Tyr Pro Arg Phe
130 135 140
Arg Lys Lys Gly Arg Thr Arg Arg Ser Phe Thr Leu Phe His Asp Val
145 150 155 160
Lys Thr Pro Thr Leu Arg Pro Asp Gly Tyr Arg Arg Leu Val Leu Pro
165 170 175
Glu Lys Ile Ser Gly Arg Arg Gly Gly Ser Val Arg Leu His Gly Asn
180 185 190
Ile Arg Leu Leu Ala Arg Arg Ile Asn Asn Gln Thr Ala Trp Ile Arg
195 200 205
Ser Val Thr Ile Ser Tyr Thr Gly Lys Tyr Trp Trp Ala Ser Ile Leu
210 215 220
Val Asp Glu Arg Ile Asp Gln Pro Ala His Pro Thr Pro Arg Gln Arg
225 230 235 240
Ala Gly Gly Ser Val Gly Met Asp Leu Gly Val His Cys Leu Ala Ala
245 250 255
Leu Ser Thr Gly Glu Val Ile Ala Asn Pro Arg His Leu Lys Thr Ala
260 265 270
Ala Asn Arg Leu Ala Lys Ala Gln Arg Gln Leu Ala Arg Thr Gly Trp
275 280 285
Trp Ile Leu Asn Ser Ser Gly His Pro Thr Gln Leu Ser Arg Arg Arg
290 295 300
Pro Ser Gln Gly Val Ala Tyr Lys Pro Thr Thr Gly Arg Leu Lys Ala
305 310 315 320
Arg Ala Arg Val Ala Arg Leu His Ala Glu Leu Ala Asp Arg Arg Ala
325 330 335
Thr Thr Gln His Ala Leu Thr Lys His Leu Val Ala Asn Tyr Asn Arg
340 345 350
Ile Ala Ile Glu Asp Leu Asn Val Ala Gly Met Thr Arg Ser Ala Lys
355 360 365
Gly Ser Gln Glu Ala Pro Gly Ser Asn Val Ala Ala Lys Ala Gly Leu
370 375 380
Asn Arg Ser Ile Leu Asp Leu Gly Phe Gly Glu Ile Arg Arg Gln Leu
385 390 395 400
Glu Tyr Lys Thr Gly Trp Tyr Gly Ser Gln Leu Val Val Ala Asp Arg
405 410 415
Tyr Ala Pro Thr Ser Lys Met Cys Ser Val Cys Arg Ala Val Lys Pro
420 425 430
Lys Leu Ser Leu Ala Glu Arg Thr Tyr Thr Cys Asn Trp Cys Gly Thr
435 440 445
Asn Ile Asp Arg Asp Leu Asn Ala Ala Cys Asn Ile Leu Asn Val Ala
450 455 460
Thr Asn Ala Asn Asn Ser Asp Ala Cys Gly Thr Gln Glu Ser Arg Asn
465 470 475 480
Ala Arg Pro Ala Thr Ser Arg Ala Ser Ala Gln Pro Val Ala Thr Thr
485 490 495
Leu Asp Gly Gln Ala Ile Thr Ser Gln Val Thr Pro Ala Lys Gln Leu
500 505 510
Ala Gly His Pro Thr Gln Thr Glu Ala Lys Arg Tyr Lys Pro Asp Thr
515 520 525
<210> 77
<211> 741
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 77
Met Pro Ile Ile Ser Pro Gly Asn Gln Glu Ser Asn Ala Asp Gly Ala
1 5 10 15
Ile Glu Leu Arg Ile Ala Ala Asp Ile Asp Glu Thr Thr Gly Cys Thr
20 25 30
Phe Ser Ala Thr Gly Leu Pro Asp Gly Leu Glu Phe Glu Arg Lys Lys
35 40 45
Phe Glu Asp Asp Glu Glu His Thr Trp Gln Thr Gly Arg Ile Ile Gly
50 55 60
Thr Pro Lys Arg Ala Gly Ile Tyr Gln Val Thr Val Thr Ala Ile Asp
65 70 75 80
Lys Ala Gly Thr Lys Thr Thr Ala Ser Phe Val Trp Thr Val Cys Ala
85 90 95
Met Arg Ala Tyr Arg Phe Ala Leu Asp Leu Ser Asp Pro Gln Ile Thr
100 105 110
Ala Met Gln Gln His Ala Gly Thr Ala Arg Trp Ala Tyr Asn Lys Ala
115 120 125
Leu Glu Ala Lys Val Asn Ala His Arg Ala Tyr Thr Glu Arg Leu Lys
130 135 140
Ala Leu Leu Ala Ser Gly Leu Thr Pro Gln Gln Ala Lys Ala Gln Met
145 150 155 160
Lys Lys Glu Ser Ala Ala Leu Lys Ala Gln Thr Ala Ile Trp Glu His
165 170 175
His Arg Gln Cys Leu Thr Ala Met Asn Leu Gly Arg Ala Leu Pro Ala
180 185 190
Lys Thr Pro Arg Gly Pro Gln Glu Leu Ile Asp Asp Leu Ala Ala Ile
195 200 205
Arg Ala Met Val Pro Asp Gly Val Asp Pro Ala Leu Leu Thr Leu Val
210 215 220
Gly Asn Trp Lys Gly Gln Pro Pro Thr Ala Leu Cys Trp Ala Arg Asp
225 230 235 240
Arg His Glu Ala Arg Gln Lys Trp Ile Lys Thr His Leu Val Met Ala
245 250 255
Arg Gln Thr Val Thr His Leu Lys Gln Gln Leu Phe Asp Leu Gly Asp
260 265 270
Thr Ile Pro Asn Met Ile Glu Asn Leu Ala Asn Trp Arg Asn Thr Arg
275 280 285
Asp Leu Pro Lys Glu Glu Gly Gly Ser Pro Trp Trp Gln Glu Val Ser
290 295 300
Ser Tyr Val Phe Ser Gly Gly Met Gly Arg Ala Asp Ala Ala Trp Ser
305 310 315 320
Asn Trp Leu Lys Ser Leu Ser Gly Gln Arg Ala Gly Lys Ala Val Gly
325 330 335
Tyr Pro Arg Phe Lys Lys Arg Gly Gln Cys Ala Asp Ser Phe Pro Leu
340 345 350
Phe His Asp Val Thr Asn Pro Ser Ile Arg Ile Glu Gly Tyr Arg His
355 360 365
Leu Arg Leu Pro Asn Ile Gly Glu Val Arg Val His Asn Ser Gly His
370 375 380
Arg Ser Gly Thr Arg Asn Arg Val Thr Ala Thr Asn Ala Lys Gly Val
385 390 395 400
Lys Arg Thr Val Ser Gly Gln Ser Lys Gly Arg Leu Gly Ser Thr Lys
405 410 415
Arg Leu Val Arg Met Ile Glu Arg Gly Asp Ala Ile Val Gln Ser Val
420 425 430
Thr Val Val Arg Gly Gly His Arg Trp Tyr Ala Asn Val Leu Val Lys
435 440 445
Leu Thr Pro Glu Ala Met Arg Gln Arg Glu Met Val Ala Arg Ala His
450 455 460
Gln Lys Val Ser Lys Lys Arg Ala Gln Gly Arg Gly Asn Val Gly Val
465 470 475 480
Glu Trp Gly Val Arg Thr Leu Ala Thr Leu Ser Asn Gly Glu Ser Phe
485 490 495
Ala Asn Pro Lys Pro Gly Lys Lys Asn Gln Arg Arg Leu Arg Val Ala
500 505 510
Ser Gln Lys Val Ser Arg Lys Pro His Lys Arg Gly Gln Pro Ile Ser
515 520 525
Ala Asn Arg Ala Ala Ala Ile Arg Gln Leu Gly Arg Val His His Glu
530 535 540
Ile Ala Glu Thr Arg Lys Gly Asn His His Gln Ile Ser Ala Ala Ile
545 550 555 560
Ser Leu Gly Tyr Arg Gly Val Ala Val Arg Gly Leu Asp Val Lys Ser
565 570 575
Met Thr Lys Ser Ala Lys Gly Thr Val Glu Lys Pro Gly Lys His Val
580 585 590
Lys Val Lys Ser Thr Phe Asn Arg Ala Ala Leu Asp Ala Ala Pro Gly
595 600 605
Met Leu Gln Asn Gln Ile Ser Tyr Lys Thr Thr Gln Tyr Gly Ala Lys
610 615 620
Phe Gly Val Ala Pro Lys Asp Leu Pro Ser Ser Gln Thr Cys Ser Ala
625 630 635 640
Cys Gly Trp Arg Asp Pro Ser Ile Pro Leu Thr Gln Met Thr Phe Arg
645 650 655
Cys Gly Asn Val Glu Cys Gly Lys Lys Ile Asp Arg Glu Val Asn Ala
660 665 670
Ala Arg Asn Ile Arg Asn Ser Val Phe Pro Val Ala Ser Asp Ser Gly
675 680 685
Asp Thr Leu Asn Ala Cys Val Asp Gly Ile Gly Pro Pro Gly Ser Arg
690 695 700
Gly Gln Pro Ser Pro Lys Gln Glu Asp Pro Glu Pro His Pro Pro Pro
705 710 715 720
Glu Asp Gly Pro Ala Glu Gly His Ser Gly Glu Ala Ile Pro Arg Arg
725 730 735
Ser His Thr Pro Gly
740
<210> 78
<211> 611
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 78
Met Pro Gln Gln Gln Pro Asp Lys Pro Ala Thr Val Thr Lys Val Ile
1 5 10 15
Lys Phe Met Val Asp Met Lys Ala Met Pro Asp Thr Gln Arg Glu Leu
20 25 30
Leu Glu Arg His Ala Gly Thr Ala Arg Ala Ala Trp Asn Trp Gly Leu
35 40 45
Ala Glu Val Asn Ala Gln Gln Arg Leu Val Gln Ala His Val Lys Arg
50 55 60
Gln Ala Leu Ala Glu Ser Ser Asp Asp Trp Asp Ala Ala Lys Lys Leu
65 70 75 80
Leu Ala Asp Lys Lys Trp Arg Thr Ala Ala Tyr His Met Ala Glu Glu
85 90 95
Gln Leu Gly Lys Val Pro Asn Ala Val Ala Leu Ser Ala Arg Phe Thr
100 105 110
Glu Met Gly Arg Asp Pro Gln Ser Arg Phe Gly Trp Trp Ser Thr Glu
115 120 125
Lys His Gly Val Asn Arg Phe Ala Ala Ser Thr Ala Leu Arg Asp Leu
130 135 140
Asp Thr Ala Arg Xaa Arg Phe Leu Ser Gly Ala Ala Lys Arg Gly Lys
145 150 155 160
Gly Arg Lys Lys Arg Ala Asp Gly Phe Pro Gly Gly Trp Pro Arg Phe
165 170 175
Lys Lys Lys Gly Arg Ser Gln Asp Ala Phe Ala Leu Phe Asn Ile Ala
180 185 190
Val Ser Asp Ser Trp Lys Pro Leu Gln Arg Arg Gly Ala Ser Lys Lys
195 200 205
Ala Pro Leu Asn His Ser Gly Glu Pro Met Leu Glu Ser His Arg Ile
210 215 220
Asn Leu Pro Ser Leu Gly Ser Val Arg Val Thr Gln Asn Thr Thr Lys
225 230 235 240
Leu Arg Arg Met Val Arg Arg Gly Gly His Pro Lys Ser Ala Arg Phe
245 250 255
Val Arg Asn Gly Asp Arg Trp Glu Ile Ala Ile Asn Val Ser Phe Gln
260 265 270
Ala Asp Asn Pro Tyr Val Ala Pro Pro Thr Lys Pro Asn Arg Ile Gln
275 280 285
Ile Ala Gly Gly Val Thr Gly Ile Asp Leu Gly Leu Gly Ala Leu Ile
290 295 300
Ala Phe Pro Asp Gly Arg Leu Ile Glu Asn Pro Gln Val Gly Lys Lys
305 310 315 320
Glu Gln Ala Lys Leu Ala Arg Leu Arg Arg Lys Tyr Ser Arg Gln His
325 330 335
Val Thr Glu Ser Pro Glu Cys Phe Glu Gly Ser Gly Val His Val Lys
340 345 350
Gly Ser Cys Ala Trp Arg Gly Gly Lys Gly Val Pro Ala Lys Met Thr
355 360 365
Ser Ser Ser Arg Glu Thr Leu Arg Gly Ile Arg Lys Thr Glu Arg Leu
370 375 380
Val Ala Gln Gln Arg Ala Thr Val Ile His Gln Gln Thr Lys Met Leu
385 390 395 400
Thr Thr Gln Phe Ala Thr Val Cys Ile Glu Asp Leu Arg Val Ala Asn
405 410 415
Met Ser Ala Arg Pro Lys Ala Lys Asp Asn Gly Asp Gly Ser Phe Ala
420 425 430
Gln Asn Gly Arg Ser Arg Lys Lys Gly Leu Asn Lys Ser Ile Leu Arg
435 440 445
Ser Ser Phe Tyr Glu Phe Arg Arg Gln Leu Glu Tyr Lys Ser Lys Leu
450 455 460
Tyr Gly Ser Lys Val Val Val Ile Asp Arg Trp Thr Pro Thr Ser Lys
465 470 475 480
Thr Cys Ser Ser Cys Gly Ser Val Lys Pro Lys Leu Lys Leu Ser Glu
485 490 495
Arg Ile Tyr Arg Cys Gln Asn Cys Gly Leu Val Met Asp Arg Asp Val
500 505 510
Asn Ala Ala Arg Asn Ile Leu Ser Ala Gly Leu Ala Glu Met Glu Arg
515 520 525
Arg Gly Ala Glu Arg Val Val Gln Thr Arg Val Pro Gly Asp Ala Val
530 535 540
Ser Gly Ser His Leu Ala His Gly Pro His Thr Gly Thr Glu Pro Cys
545 550 555 560
Gly Thr Thr Val Gly Lys Thr Pro Lys Pro Ser Glu Thr Ala Lys His
565 570 575
Ser Arg Arg Gly Pro Gly Leu Ala Asn Asp Ala Thr Gly Ser Ser Asn
580 585 590
Gly Ala Val Thr Pro Thr Gln Cys Arg Ser Gly Thr Thr Arg Asn Arg
595 600 605
Arg Ala Ala
610
<210> 79
<211> 594
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 79
Met Pro Gln Thr Glu Val Leu Arg Ala Tyr Arg Phe Ala Leu Asp Pro
1 5 10 15
Ser Asp Ala Glu Arg Ala Glu Leu Ala Arg Tyr Ala Gly Ala Cys Arg
20 25 30
Trp Ala Phe Asn Tyr Ala Leu Ala Lys Lys Thr Gln Ser Tyr Arg Ala
35 40 45
Trp Ala Glu Arg Arg Thr Ala Tyr Leu Ala Ala Gly Leu Ser Glu Ala
50 55 60
Glu Ala Lys Ala Arg Ile Lys Ala Asp Gly Ala Glu Leu Thr Asp Arg
65 70 75 80
Ile Lys Val Trp Asp His His Arg Lys Cys Leu Met Asn Thr Ala Thr
85 90 95
Gly Lys Gln Pro Leu Pro Ala Thr Gln Pro Pro Ala Gly Gln Glu Ala
100 105 110
Leu Ala Cys Arg Leu Ala Ala Ala Arg Ala Asp Ala Ala Ala Thr Gly
115 120 125
Cys Glu Arg Arg Leu Leu Ala Glu Ala Arg Thr Thr Val Asn Ala Leu
130 135 140
Lys Ala Glu Ala Phe Ser Ala Gly Tyr Arg Thr Pro Asn Ala Thr Asp
145 150 155 160
Thr Ser Ala Leu Trp Arg Met Asp Arg Asp Arg Pro Lys Glu Glu Gly
165 170 175
Gly Ser Pro Trp Trp Thr Glu Val Asn Val Tyr Cys Phe Thr Ser Gly
180 185 190
Phe Asp Arg Ala Gln Val Ala Trp Lys Asn Trp Gln Asp Ser Leu Ala
195 200 205
Gly Arg Arg Ala Gly Cys Arg Asn Arg Tyr Pro Arg Phe Lys Lys Lys
210 215 220
Ser Arg Ala Ala Glu Ser Phe Thr Leu Phe His Asp Val Lys Arg Pro
225 230 235 240
Ile Ile Arg Leu Asp Gly Tyr Arg Arg Leu Val Met Pro Gly Leu Gly
245 250 255
Ser Ile Arg Ile His Asp Gly Gly Lys Arg Leu Ala Arg Leu Val Asp
260 265 270
Arg Gly Gln Ala Val Ile Gln Ser Val Thr Val Thr Arg Gly Gly His
275 280 285
Arg Trp Tyr Ala Ser Val Leu Ala Lys Val Gln Gln Asp Val Pro Val
290 295 300
Leu Trp Glu His Val Asp Asp Ala Gly Thr Arg Val Arg Tyr Leu Thr
305 310 315 320
Arg Thr Gln Ala Glu Asn Ala Ala Glu Asn Gly Gly Arg Ile Glu Gln
325 330 335
Ile Gly Arg Pro Ser Ala Arg Gln Arg Ala Gly Gly Leu Val Gly Val
340 345 350
Asp Leu Gly Ser Arg Tyr Leu Ala Ala Leu Ser Ser Pro Leu Asp Pro
355 360 365
Ala Asp Pro Thr Ser Ile Leu Leu Pro His Pro Arg Leu Leu Ala Asp
370 375 380
Ser Thr Ala Lys Leu Ala Lys Ala Gln Arg Thr Leu Ser Arg Thr Gln
385 390 395 400
Arg Gly Ser Arg Arg Arg Val Lys Ala Ala Ala Arg Val Ala Arg Val
405 410 415
His His Gln Ile Lys Val Arg Arg Ala Ser Phe Leu His Gly Leu Ser
420 425 430
Lys Lys Leu Ala Thr Arg Phe Thr His Val Ala Ile Glu Asp Leu Asn
435 440 445
Leu Thr Ala Leu Thr Ala Ser Ala Lys Gly Ser Pro Asp Lys Pro Gly
450 455 460
Lys Asn Val Lys Val Thr Ala Arg Phe Asn Arg His Leu Leu Asp Ala
465 470 475 480
Ala Leu Gly Ser Leu Arg Ser Arg Leu Ala Tyr Lys Thr Ala Trp Tyr
485 490 495
Gly Ser Gln Leu Val Ile Leu Asp Lys Ala Glu Pro Val Ala Ser Thr
500 505 510
Cys Ala Lys Cys Lys Glu Arg Asn Pro Asn Ser Ala Pro Ser His Ser
515 520 525
Lys Phe His Cys Pro Ser Cys Gly Ala Val Val His Arg His Glu Asn
530 535 540
Ser Thr Ala Asn Ile Val Arg Ala Ala Arg Arg Gln Leu Thr Thr Val
545 550 555 560
Ala Ser Asp Arg Gly Glu Thr Gln Asn Ala Arg Arg Ala Ala Val Ser
565 570 575
Pro Gly Ala Arg Lys Gly Pro Gly Gln Gly Ala Leu Lys Arg Glu Asp
580 585 590
Thr Gly
<210> 80
<211> 593
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 80
Met Pro Arg Thr Glu Val Leu Arg Ala Tyr Arg Phe Ala Leu Asp Ala
1 5 10 15
Ser Asp Ala Gln Arg Ala Ala Leu Ser Arg Tyr Ala Gly Ala Cys Arg
20 25 30
Trp Ala Tyr Asn Tyr Ala Leu Ala Lys Lys Thr Arg Ala His Gln Ala
35 40 45
Trp Ala Glu Arg Arg Thr Ala Tyr Leu Glu Ala Gly Leu Ser Glu Ala
50 55 60
Glu Ala Lys Glu Arg Ile Lys Ala Asp Gly Ala Glu Leu Thr Asp Arg
65 70 75 80
Ile Lys Val Trp Asp His His Arg Lys Ser Leu Ala Leu Thr Val Ala
85 90 95
Gly Lys Pro Pro Leu Pro Ala Met Gln Ser Pro Ala Gly Gln Gly Val
100 105 110
Leu Ile Arg Arg Leu Ala Ala Val Arg Ala Asp Ala Ala Gly Ser Gly
115 120 125
Ser Glu Arg Glu Leu Leu Ala Ala Ala Arg Ala Thr Val Asn Ala Leu
130 135 140
Lys Val Glu Ala Phe Ser Ala Gly Phe Arg Thr Pro Thr Ser Thr Asp
145 150 155 160
Thr Ser Ala Leu Trp Arg Ile Glu Arg Asp Arg Pro Arg Lys Glu Gly
165 170 175
Gly Ser Pro Trp Trp Ser Glu Val Asn Val Tyr Cys Phe Thr Ser Gly
180 185 190
Phe Asp Arg Ala Gln Val Ala Trp Arg Tyr Trp Gln Asp Ser Leu Ala
195 200 205
Gly Arg Arg Ala Gly Gln Arg Thr Gly Tyr Pro Arg Phe Lys Lys Lys
210 215 220
Gly His Ala Glu Ser Phe Ala Leu Phe His Asp Val Lys Arg Pro Ile
225 230 235 240
Ile Arg Leu Asp Gly Tyr Arg Arg Leu Val Met Pro Gly Leu Gly Ser
245 250 255
Ile Arg Ile His Asp Ser Gly Lys Arg Leu Ala Arg Leu Val Asp Arg
260 265 270
Gly Gln Ala Val Ile Gln Ser Val Thr Val Thr Arg Gly Gly His Arg
275 280 285
Trp Tyr Ala Ser Val Leu Ala Lys Val Gln Gln Asp Val Pro Val Leu
290 295 300
Trp Glu His Val Asp Asp Asp Gly Thr Arg Thr Pro Tyr Leu Ser Arg
305 310 315 320
Thr Gln Ala Glu Thr Ala Ala Glu Asn Gly Gly Arg Val Glu Gln Ile
325 330 335
Gly Arg Pro Thr Ala Arg Gln Arg Ala Gly Asp Leu Val Gly Val Asp
340 345 350
Leu Gly Ser Pro Tyr Leu Ala Ala Leu Ser Ser Pro Leu His Pro Ala
355 360 365
Asp Pro Ala Thr Ala Leu Val Pro His Pro Arg Leu Leu Ala Asp Ser
370 375 380
Leu Pro Lys Leu Ala Lys Ala Gln Arg Ala Met Ser Arg Cys Gln Gln
385 390 395 400
Gly Ser Glu Arg Trp Arg Lys Ala Thr Ala Gln Val Ser Arg Leu His
405 410 415
Gln Gln Ile Thr Val Arg Arg Ala Ser Phe Leu His Gly Leu Ser Lys
420 425 430
Thr Leu Ala Thr Gly Phe Thr His Val Ala Ile Glu Asp Leu Arg Leu
435 440 445
Thr Ala Leu Leu Thr Ser Ala Lys Gly Asn Pro Asp Lys Pro Gly Lys
450 455 460
Ala Ala Lys Arg Lys Ser His Ser Asn Arg His Leu Leu Glu Thr Gly
465 470 475 480
Gln Gly Ser Leu Arg Lys Lys Leu Glu Tyr Lys Thr Ala Trp His Gly
485 490 495
Ser Gln Leu Val Val Leu Asp His Arg Glu Pro Val Ala Gly Thr Cys
500 505 510
Ser Lys Cys Lys Glu Arg Asn Pro Ser Ser Asp Ser Ser His Ser Lys
515 520 525
Phe His Cys Leu Ser Cys Gly Ala Val Val His Arg His Glu Asn Asn
530 535 540
Thr Ala Asn Ile Val His Thr Ala Leu Arg Gln Leu Met Thr Val Ala
545 550 555 560
Ser Asp Arg Gly Glu Thr Gln Asn Ala Arg Arg Ala Thr Val Ser Pro
565 570 575
Leu Ala Arg Lys Gly Pro Gly Arg Glu Ala Leu Lys Arg Glu Asp Thr
580 585 590
Gly
<210> 81
<211> 493
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 81
Met Pro Thr Glu Ile Arg Thr Ile Gln Gln Ala Tyr Arg Phe Ala Leu
1 5 10 15
Asp Pro Thr Pro Ala Gln Glu Arg Met Leu Arg Ser His Ala Gly Ala
20 25 30
Ser Arg Phe Ala Tyr Asn Trp Gly Asn Ala Arg Ile Ile Lys Ala Leu
35 40 45
Asp Ala Arg Lys Ala Glu Glu Ala Ala Gly Gly Glu Ala Lys Thr Lys
50 55 60
Val Pro Gly His Phe Asp Leu Cys Lys Gln Trp Thr Ser Trp Lys Asp
65 70 75 80
Glu His Ala Asp Asp Pro Glu Pro Ala Glu Gly Glu Arg Arg Thr Ser
85 90 95
Thr Ala Trp Val Ser Glu Asn Phe Val Gly Thr Tyr Gln Ala Ala Leu
100 105 110
Arg Asp Ala Ala Lys Ala Trp Gly Asp Phe Phe Lys Ser Ala Lys Gly
115 120 125
Glu Arg Lys Gly Arg Arg Leu Gly Arg Pro Arg Phe Lys Lys Arg Gly
130 135 140
Lys Ala Arg Asp Ser Phe Gln Val His Gly Gly Thr Leu Arg Ile Glu
145 150 155 160
Ser Ala Gly Ala Gly Phe Arg Pro Lys Gly Ala Asp Lys Gly Ala Thr
165 170 175
Arg Asp Met Val Val Leu Pro Lys Ile Gly Pro Val Phe Leu His Glu
180 185 190
Ser Ala Arg Lys Leu Arg Arg Arg Val Asp Ala Gly Thr Ala Arg Ile
195 200 205
Val Arg Gly Thr Ile Ala Arg Glu Ser Asp Gly Arg Trp His Ile Ala
210 215 220
Ile Thr Ala Glu Val Arg Gln Glu Val Arg Thr Gly Pro Ser Ala Arg
225 230 235 240
Gln Arg Ala Gly Gly Ile Ala Gly Ala Asp Phe Gly Val Arg Asp Ile
245 250 255
Ala Thr Leu Ser Asp Gly Thr Ala Ile Ala Asn Pro Arg His Leu Glu
260 265 270
Gly Ala Leu Gly Lys Leu Ala Lys Ala Gln Arg Ala Leu Ser Arg Cys
275 280 285
Glu Lys Asp Ser Ala Gly Arg Arg Lys Ala Val Ala Arg Val Gly Arg
290 295 300
Leu His Ala Arg Val Arg His Leu Arg Leu Asp Gly Leu His Lys Ala
305 310 315 320
Thr Ser Gly Leu Val His Ser Arg Ala Val Leu Ala Val Glu Gly Phe
325 330 335
Asp Val Gln Glu Thr Ala Arg Tyr Arg Arg Arg Gly Lys Ala Asp Pro
340 345 350
Gly Lys Tyr Arg Glu Asn Val Pro Ala Arg Val Arg Ala Asp Arg Asn
355 360 365
Arg Ala Leu Ala Asp Thr Gly Ile Gly Ile Ala Arg Trp Gln Leu Gln
370 375 380
Ser Lys Gly Ala Trp Tyr Gly Cys Gln Val Leu Val Met Asp Lys His
385 390 395 400
Ala Pro Thr Gly Arg Thr Cys Ser Ala Cys Gly Thr Ala Lys Ala Lys
405 410 415
Pro Val Pro Pro Ala Asp Glu Leu Phe Thr Cys Glu Ala Cys Gly Ser
420 425 430
Met Met Pro Arg Arg Val Asn Thr Ala Arg Ala Leu Ala Leu Arg Ala
435 440 445
Arg Val Ala Val Thr Val Ala Pro Ser Thr Gly Glu Thr Leu Asn Ala
450 455 460
Arg Gly Gly Asp Val Arg Pro Gly Ala Ala Arg Gly Asn Arg Gln Pro
465 470 475 480
Pro Leu Lys Arg Glu Ala Ser Thr Ser Pro Arg Ala Gly
485 490
<210> 82
<211> 612
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 82
Met Pro Thr Glu Ile Arg Thr Ile Gln Gln Ala Tyr Arg Phe Ala Leu
1 5 10 15
Asp Pro Thr Pro Arg Gln Glu Arg Met Leu Arg Ser His Ala Gly Ala
20 25 30
Ala Arg Phe Ala Tyr Asn Trp Gly Ile Ala Arg Thr Val Asn Ala Leu
35 40 45
Asp Ala Arg Glu Ala Glu Lys Ala Ala Val Gln Ser Ala Ala Ala Glu
50 55 60
Ala Ala Ala Arg Val Ala Glu Leu Glu Gln Leu Val Ala Asp Ala Ala
65 70 75 80
Ala Asp Ala Ala Ala Glu Tyr Thr Pro Pro Met Thr Ala Val Gly Asp
85 90 95
Ala Thr Ile Arg Leu Arg Asn Leu Gln Ala Gln Leu Ala Gly Ala Lys
100 105 110
Ala Asp Ala Ala Thr Ala Ser Gly Gly Lys Pro Glu Thr Lys Val Pro
115 120 125
Gly His Phe Asp Leu Cys Lys Gln Trp Thr Ala Trp Lys Asp Glu His
130 135 140
Ala Ser Asp Pro Glu Pro Glu Pro Gly Gly Arg Arg Thr Asn Thr Ala
145 150 155 160
Trp Val Ala Glu Asn Phe Val Gly Thr Tyr Gln Ala Ala Leu Arg Asp
165 170 175
Ala Ala Lys Ala Trp Ala Asp Phe Phe Lys Ser Ala Lys Gly Glu Arg
180 185 190
Lys Gly Arg Arg Leu Gly Arg Pro Lys Phe Lys Lys Arg Gly Lys Ala
195 200 205
Lys Ala Arg Asp Ser Phe Gln Val His Gly Gln Thr Leu Arg Val Val
210 215 220
Ser Ala Gly Ala Gly Phe Arg Pro Lys Gly Ala Asp Arg Gly Ala Thr
225 230 235 240
Arg Asp Met Val Val Leu Pro Lys Ile Gly Pro Val Phe Leu His Glu
245 250 255
Ser Gly Arg Lys Leu Arg Arg Arg Thr Asp Lys Gly Thr Ala Arg Ile
260 265 270
Val Arg Gly Thr Ile Ala Arg Glu Ser Asp Gly Arg Trp Tyr Ile Ala
275 280 285
Leu Thr Ala Glu Val Gln Arg Glu Val Arg Thr Gly Pro Ser Ala Arg
290 295 300
Gln Arg Glu Gly Gly Val Val Gly Ala Asp Phe Gly Val Arg Glu Leu
305 310 315 320
Ala Thr Leu Ser Ser Gly Asn Val Ile Pro Asn Pro Arg His Leu Glu
325 330 335
Ala Asp Leu Gly Arg Leu Ala Gln Ala Gln Arg Ala Leu Ser Arg Cys
340 345 350
Glu Pro Gly Ser Lys Arg Arg Ala Lys Ala Val Arg Arg Val Gly Arg
355 360 365
Leu His Ala Arg Val Arg Ala Glu Arg Leu Asp Ser Leu His Lys Ala
370 375 380
Thr Ser Ala Leu Val His Ser His Glu Arg Ile Val Val Glu Gly Phe
385 390 395 400
Asp Val Gln Glu Thr Ala Arg Tyr Arg Lys Arg Gly Lys Val Asp Pro
405 410 415
Lys Lys Lys Glu Lys Ser Lys Ser Val Pro Gly Lys Val Arg Ala Asp
420 425 430
Arg Asn Arg Ala Leu Ala Asp Thr Gly Ile Gly Ile Ala Arg Trp Gln
435 440 445
Leu Glu Ser Lys Ser Ala Trp Tyr Gly Cys Gln Val Val Lys Thr Asp
450 455 460
Lys His Ala Pro Thr Gly Arg Ala Cys Ser Ala Cys Gly Thr Val Lys
465 470 475 480
Ala Lys Pro Val Pro Pro Ala Asp Glu Leu Phe Thr Cys Glu Ala Cys
485 490 495
Gly Thr Arg Met Pro Arg Arg Leu Asn Thr Ala Arg Val Leu Leu Ser
500 505 510
Val Thr Val Ala Ala Ser Thr Arg Glu Thr Leu Arg Arg Arg Val Gly
515 520 525
Ala Ala Val Thr Gly Thr Ala Thr Pro Ala Gly Glu Ala Val Lys Glu
530 535 540
Thr Ile Asn Ala Pro Gly Gly Ser Val Ser Pro Ser Ala Ala Arg Gly
545 550 555 560
Gly Gly Gln Phe Pro Val Lys Gly Glu Ala Ser Thr Arg Lys Gly Thr
565 570 575
Thr Leu Ala Glu Asn Ala Ala Ser Arg Val Pro Gly Glu Asn Ala Met
580 585 590
Ser Tyr Ser Gly His Arg Ala Ala Gly Ile Glu His Arg Ala Ala Glu
595 600 605
Pro Arg Pro Gly
610
<210> 83
<211> 506
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 83
Met Pro Thr Glu Met Arg Leu Ala Gln Arg Ala Phe Arg Phe Ala Leu
1 5 10 15
Asp Pro Thr Pro Arg Gln Ala Arg Met Phe Ala Ser His Ala Gly Gly
20 25 30
Ala Arg Phe Ala Tyr Asn Trp Gly Asn Ala Gln Ile Ile Ala Ala Leu
35 40 45
Asp Ala Arg Gln Ala Glu Lys Asp Ala Gly Ala Glu Pro Thr Val Lys
50 55 60
Thr Pro Ser His Phe Asp Leu Cys Lys Ala Trp Thr Ala Trp Lys Asn
65 70 75 80
Glu His Ala Ala Asp Pro Glu Pro Ala Asp Gly Glu Arg Arg Thr Asn
85 90 95
Thr Ala Trp Val Ala Glu Asn Phe Val Gly Thr Tyr Gln Ala Ala Leu
100 105 110
Arg Asp Ala Ala Lys Ala Trp Ser Asp Phe Phe Ala Ser Arg Ser Gly
115 120 125
Lys Arg Lys Gly Arg Arg Leu Gly Arg Pro Arg Phe Lys Lys Lys Gly
130 135 140
Lys Ser Arg Asp Ser Phe Gln Val His Gly Gly Thr Leu Arg Met Glu
145 150 155 160
Asp Ala Val His Val Thr Leu Pro Lys Ile Gly Thr Ile Thr Val Met
165 170 175
Ser Asp Asp Ser Leu His Pro Ala Met Arg Arg Ser Arg Ala Arg Ala
180 185 190
Thr His Leu Ala Pro Arg His Met Gly Asn Arg Arg Arg Ala Arg Thr
195 200 205
Leu Trp Arg Ala Leu Arg Arg Gly Gly Gly Ala Arg Leu Ile Arg Gly
210 215 220
Thr Val Thr Arg Gly Ala Asp Gly Leu Trp Trp Leu Ser Leu Thr Ala
225 230 235 240
Glu Ile Pro Leu Gln Val Arg Ala Ser Pro Ser Arg Ala Gln Gln Glu
245 250 255
Gly Gly Met Ile Gly Ala Asp Phe Gly Val Arg Glu Val Ala Thr Phe
260 265 270
Ser Asn Gly Val Thr Ile Arg Asn Ser Arg His Leu Glu Ala Ala Leu
275 280 285
Ala Glu Leu Arg Ala Ala Gln Lys Ala Leu Ser Arg Cys Gln Lys Asp
290 295 300
Ser Met Arg Arg Glu Lys Ala Lys Ala Arg Ile Gly Val Ile His Ala
305 310 315 320
Asp Val Ala Arg Leu Arg Glu Thr Ala Leu His Arg Ala Thr Thr Val
325 330 335
Leu Val Arg Gln His Asp Val Ile Ala Met Glu Gly Trp Asp Val Gln
340 345 350
Gln Val Met Arg Gly Gly Ser Lys Asp Val Pro Arg Arg Ile Arg Arg
355 360 365
Asp Arg Asn Arg Ala Leu Ala Asp Thr Gly Val Gly Met Ala Arg Gln
370 375 380
Met Leu Ser Tyr Lys Gly Pro Gln Met Gly Ala Ile Val Met Val Thr
385 390 395 400
Asp Pro Gln Ala Lys Thr Gly Arg Thr Cys Ser Val Cys Arg Thr Pro
405 410 415
Arg Thr Thr Ala Leu Pro Pro His His Glu Met Phe Thr Ser Asp Gln
420 425 430
Cys Gly His Val Leu Asp Arg Arg Leu Asn Thr Ala Gln Ala Leu Ala
435 440 445
Gly Trp Ala Ala Gln Glu Leu Ser Lys Arg Gly Pro Ala Pro Ala Gly
450 455 460
Pro Ser Arg Pro Arg Gly Gly Asn Val Arg Pro Ala Ala Asp Arg Lys
465 470 475 480
Val Gly Gly Gly Arg Ser Pro Val Lys Arg Ala Ala Ser Thr Arg Leu
485 490 495
Gly Arg Gly Glu Pro Gly Thr Pro Gly Gly
500 505
<210> 84
<211> 607
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 84
Met Pro Thr Glu Pro Arg Leu Ile Gln Lys Ala Tyr Lys Phe Ala Leu
1 5 10 15
Asp Pro Thr Pro Thr Gln Ala Arg Ala Phe Ala Ser His Ala Gly Gly
20 25 30
Ala Arg Phe Ala Tyr Asn Trp Gly Ile Ser Thr Tyr Ala Ala Ala Leu
35 40 45
Asp Ala Arg Lys Ala Gln Thr Asp Ala Gly Gln Asp Pro Thr Val Asp
50 55 60
Val Pro Asn His Phe Ala Leu Cys Lys Ala Trp Thr Gln Tyr Lys Asp
65 70 75 80
Thr Tyr Arg Leu Thr Gly Asp Pro Asp Gly Arg Pro Val His Trp Val
85 90 95
Glu Asp Asn Phe Val Gly Thr Tyr Gln Ala Ala Leu Arg Asp Ala His
100 105 110
Gly Ala Trp Gln Lys Met Trp Asp Ser Arg Asn Gly Gln Arg Lys Gly
115 120 125
Arg Ala Val Gly Arg Pro Arg Phe Lys Lys Lys Gly Arg Ser Pro Asp
130 135 140
Ser Phe Gln Met His Gly Asp Ser Leu Arg Leu Asp Ser Gly Arg Ala
145 150 155 160
Leu Arg Pro Ala Asp Ala Thr Ala Arg Lys Pro Leu Asn Arg Ala Glu
165 170 175
Arg Ala Lys Leu Ala Pro Thr Pro Gly Met Thr Pro Thr Glu Arg Ser
180 185 190
Ala Ala Gln Lys Arg Leu Arg Ala Gly Arg Lys Ala Arg Ala Gly His
195 200 205
Val His Gly Pro Gln Val Ser Gln Ser Gly Thr Ile Tyr Val Asn Leu
210 215 220
Pro Lys Val Gly Pro Val Arg Val Pro Gly Arg Val Arg Ile Pro Gln
225 230 235 240
Val Thr Pro Ser Val Ala Gly Trp Val Asp Arg Asn Ser Arg Thr Ala
245 250 255
Arg Lys Leu Leu Arg Gln Leu Ala Lys Ala Ala Thr Thr Gly Pro Val
260 265 270
Val Cys Pro Thr Cys Ala Gly Thr Gly Thr Val Thr Val Thr Thr Lys
275 280 285
Lys Gly Glu Arg Arg Asp Thr Lys Cys Gly Lys Tyr His Pro Thr Val
290 295 300
Arg Pro Glu Asp Lys Leu Pro Gly Cys Gln Gly Lys Gly Lys Val Glu
305 310 315 320
Pro Ala Arg Ile Val Arg Ala Thr Ile Ser Arg Gly Ala Ser Gly Thr
325 330 335
Trp Trp Cys Ser Ile Thr Thr Glu Gln Val Ile Thr Val Pro Val Ile
340 345 350
Thr Thr Arg Arg Pro Ala His Pro Thr Arg Arg Gln Leu Ala Asp Gly
355 360 365
Ser Ile Gly Val Asp Leu Gly Val Lys Tyr Leu Ala Val Leu Ser Thr
370 375 380
Gly Asp Thr Tyr Met Asn Ser Arg Phe Leu Asp Ser Ala Leu Thr Asp
385 390 395 400
Leu Lys Arg Met Gln Lys Val Leu Ser Arg Thr Ala Lys Asp Ser His
405 410 415
Arg Arg Leu Lys Ala Arg Arg Arg Val Gly Thr Leu His Glu Gln Val
420 425 430
Ala Leu Met Arg Arg Asp Ala Thr Asn Arg Val Thr Thr Glu Leu Ala
435 440 445
Arg Lys Tyr Ala Thr Ile Ser Val Glu Gly Trp Asp Ala Gln Arg Leu
450 455 460
Ala Glu Leu Gly Ser Lys Asp Leu Pro Lys Arg Leu Arg Arg Asn Arg
465 470 475 480
Asn Arg Ala Leu Ala Asp Ala Ala Pro Gly Met Leu Arg Trp Gln Leu
485 490 495
Glu Tyr Lys Thr Ser Trp Tyr Gly Ser Thr Leu Phe Lys Gly Glu Pro
500 505 510
His Trp Glu Thr Gly Arg Ile Cys Ser Val Cys Gly Thr Ala Arg Thr
515 520 525
Lys Pro Val Pro Leu Ala Glu Glu Gln Phe Arg Cys Pro Gln Gly His
530 535 540
Val Leu Pro Arg Arg Val Asn Ser Ala Arg Val Met Asp Arg Val Ala
545 550 555 560
Arg Gly Leu Val Ala Ala Pro Ala Thr Pro Glu Pro Arg Gly Gly Asp
565 570 575
Val Arg Pro Ala Thr Pro Arg Arg Gly Gly Pro Ser Pro Ala Lys Arg
580 585 590
Val Ala Arg Ser Arg Lys Arg Gly Lys Thr Gly Thr Pro Asp Pro
595 600 605
<210> 85
<211> 572
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 85
Met Pro Val Glu Ile Gln Thr Ile Gln Gln Ala Tyr Lys Phe Ala Leu
1 5 10 15
Asp Pro Thr Pro Arg Gln Ala Arg Met Leu Ala Ser His Ala Gly Ala
20 25 30
Ala Arg Phe Ala Phe Asn Trp Gly Asn Ala Arg Val Ile Ala Ala Leu
35 40 45
Asp Ala Arg Glu Ala Glu Lys Ala Ala Gly Gly Glu Ala Lys Thr Pro
50 55 60
Leu Pro Ser His Phe Asp Leu Cys Lys Ala Trp Thr Ala Trp Lys Asp
65 70 75 80
Glu His Ala Ala Asp Pro Glu Pro Pro Glu Gly Glu Arg Arg Thr Asn
85 90 95
Thr Ala Trp Val Ser Glu Asn Phe Val Gly Ser Tyr Gln Ala Ala Leu
100 105 110
Arg Asp Ala Ala Lys Ala Trp Ser Asp Phe Phe Lys Ser Arg Ser Gly
115 120 125
Lys Arg Lys Gly Arg Pro Leu Gly Arg Pro Arg Phe Lys Lys Arg Gly
130 135 140
Arg Ala Arg Asp Ser Phe Gln Val His Gly Gln Thr Leu Arg Pro Glu
145 150 155 160
Asn Cys Gly Ala Gly Phe Arg Pro Lys Gly Ala Asp Lys Gly Ala Thr
165 170 175
Arg Asp Met Ile Val Leu Pro Lys Ile Gly Pro Val Phe Leu Gly Glu
180 185 190
Ser Ala Arg Lys Leu Arg Arg Trp Leu Arg Lys Pro Asp Val Ala Cys
195 200 205
Pro Asp Cys Cys Gly Trp Gly Ser Pro Gly Lys Val Pro Ala Ala Ala
210 215 220
Asp Ser Lys Ala Lys Ala Val Ala Asp Cys Thr Ala Cys Pro Gly Ala
225 230 235 240
Gly Arg Asp Cys Pro Val Cys Asp Gly Ser Gly Lys Val Arg Glu Cys
245 250 255
Pro Ala Cys Gly Gly Ser Leu Arg Val Pro Ala Ala Arg Ile Val Arg
260 265 270
Gly Thr Val Ala Arg Glu Ser Asp Gly Arg Trp Leu Val Ser Leu Thr
275 280 285
Val Gln Arg Ala Arg Glu Val Arg Ala Val Arg Val Lys Thr Pro Leu
290 295 300
Lys Asn Gly Gly Thr Ala Arg Glu Tyr Gln Pro Ser Pro Ser Ala Arg
305 310 315 320
Gln Arg Lys Gly Gly Ile Ala Gly Val Asp Phe Gly Val Arg Asp Leu
325 330 335
Ala Thr Leu Ser Asp Gly Thr Val Val Ala Asn Pro Arg His Leu Glu
340 345 350
Gly Asp Leu Gly Arg Leu Ala Arg Ala Gln Arg Val Leu Ser Arg Arg
355 360 365
Glu Pro Gly Ser Lys Gly Arg Arg Glu Ala Val Ala Arg Val Gly Arg
370 375 380
Leu His Ala Arg Val Arg His Leu Arg Ser Asp Ser Leu His Lys Ala
385 390 395 400
Thr Ser Gly Leu Val His Ala Tyr Glu Thr Ile Ala Val Glu Gly Phe
405 410 415
Asp Val Gln Glu Thr Ala Arg Tyr Arg Arg Arg Gly Lys Ala Asp Pro
420 425 430
Lys Lys Gly Lys Asp Val Pro Ala Arg Val Arg Ala Asp Arg Asn Arg
435 440 445
Ala Leu Ala Asp Thr Gly Ile Gly Thr Ser Arg Trp Gln Leu Leu Ser
450 455 460
Lys Gly Ala Trp Tyr Gly Cys Gln Val Leu Val Met Asp Lys His Ala
465 470 475 480
Pro Thr Gly Arg Thr Cys Ser Ala Cys Gly Thr Ala Lys Ala Lys Pro
485 490 495
Val Pro Pro Ala Glu Glu Met Phe Thr Cys Gln Ala Cys Gly Asn Val
500 505 510
Met Pro Arg Arg Val Asn Thr Ala Arg Ala Leu Glu Leu Arg Ala Arg
515 520 525
Lys Ala Val His Val Ala Pro Ser Thr Gly Glu Thr Val Asn Ala Arg
530 535 540
Gly Gly Ala Val Ser Pro Ala Ala Val Thr Gly Ser Gly Gln Val Pro
545 550 555 560
Leu Lys Arg Glu Ala Ser Thr Arg Pro Arg Pro Gly
565 570
<210> 86
<211> 508
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 86
Met Pro Val Glu Asn Arg Leu Ile Gln Gln Ala Phe Val Phe Ala Leu
1 5 10 15
Asp Ala Thr Pro Arg Gln Gln Arg Ala Phe Ala Ser His Ala Gly Gly
20 25 30
Ala Arg Phe Ala Tyr Asn Trp Gly Ile Gln Lys Ile Ala Glu Ala Leu
35 40 45
Asp Ala Arg Glu Ala Glu Lys Ala Ala Gly Gln Thr Pro Ala Thr Lys
50 55 60
Ile Pro Gly His Phe Asp Leu Cys Arg Met Trp Thr Glu Trp Lys Asn
65 70 75 80
Thr Ala Gln Trp Val Asp Arg Glu Thr Gly Glu Val Thr Ala Gly Val
85 90 95
Pro Trp Val Ser Arg Asn Phe Val Gly Thr Tyr Gln Ala Ala Leu Arg
100 105 110
Asp Ala Ala Ala Ala Trp Lys Arg Phe Phe Asp Ser Arg Ser Gly Lys
115 120 125
Leu Ala Gly Arg Arg Val Gly Arg Pro Arg Phe Lys Lys Lys Gly Arg
130 135 140
Ser Arg Glu Ser Phe Gln Val His Gly Ala Gln Leu Lys Val Val Asp
145 150 155 160
Ala His His Val Gln Leu Pro Lys Ile Gly Val Val Lys Thr His Glu
165 170 175
Ser Thr Arg Lys Leu Leu Arg Arg Ile Arg Lys Gly Asp Thr Thr Cys
180 185 190
Pro Thr Cys Gln Ala Thr Gly Gln Val Pro Gly Lys Asp Thr Pro Arg
195 200 205
Lys Cys Pro Ala Cys Lys Gly His Gly Arg Val Pro Thr Ala Arg Leu
210 215 220
Val Arg Gly Thr Val Ser Arg Gly Ser Asp Gly Arg Trp Gln Ile Ala
225 230 235 240
Phe Thr Val Glu Leu Val Arg Glu Val Arg Thr Gly Pro Ser Arg Arg
245 250 255
Gln Arg Ala Gly Gly Thr Ile Gly Val Asp Leu Gly Val Arg Phe Leu
260 265 270
Ala Thr Leu Ser Thr Gly Glu Gln Val Lys His Pro Arg His Leu Glu
275 280 285
Ala Ala Leu Asp Arg Leu Ala Ala Ala Gln Arg Ala Leu Ala Arg Cys
290 295 300
Glu Lys Gly Ser Lys Arg Arg Ala Lys Ala Val Ala Arg Val Ala Arg
305 310 315 320
Leu His Ala Arg Val Ala Asn Leu Arg Arg Asp Ala Ala His Lys Phe
325 330 335
Thr Ser His Leu Val His Ser His Glu Arg Ile Ala Val Glu Gly Trp
340 345 350
Asp Val Gln Gln Val Met Gln His Gly Ser Ala Gly Leu Pro Arg Arg
355 360 365
Val Arg Arg Ala Arg Asn Arg Leu Leu Ala Asp Ala Gly Leu Gly Glu
370 375 380
Ile Arg Trp Gln Leu Lys Ser Lys Ser Ala Trp Tyr Gly Ala Thr Val
385 390 395 400
Val Val Ala Asp Pro His Ala Pro Thr Gly Arg Thr Cys Ser Arg Cys
405 410 415
Gly Gln Val Arg Ala Lys Pro Val Pro Pro Val Glu Glu Leu Phe Ala
420 425 430
Cys Pro Ser Cys Gly Trp Cys Gly Asp Arg Arg Leu Asn Thr Ala Arg
435 440 445
Val Leu Ala Gln Val Ala Arg Gly Asp Ile Val Ser Asp Ala Ser Ser
450 455 460
Gly Gly Glu Ser Leu Asn Ala Arg Gly Gly Asp Val Arg Pro Gly Ala
465 470 475 480
Leu Arg Arg Gly Gly Arg Ser Pro Val Lys Arg Glu Ala Arg Ala Trp
485 490 495
Leu Lys Ser Arg Gly Glu Thr Gly Thr Pro Gly Pro
500 505
<210> 87
<211> 542
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 87
Met Pro Val Glu Val Arg Thr Val Gln Gln Ala Tyr Arg Phe Pro Leu
1 5 10 15
Asp Pro Thr Pro Ala Gln Ala Arg Ala Phe Met Ser His Ala Gly Gly
20 25 30
Ala Arg Tyr Ala Phe Asn Trp Gly Leu Ala Thr Lys Ala Ala Ala Leu
35 40 45
Asp Ala Arg Gln Ala Gln Lys Asp Ala Gly Glu Glu Leu Ser Val Pro
50 55 60
Val Pro Asn His Phe Ala Leu Cys Leu Leu Trp Thr Glu Phe Lys Asp
65 70 75 80
His Gly Val Val Cys Arg Arg Cys Gly Met Pro Val Thr Ala Gly Gly
85 90 95
Asp Gly Asp His Ala Ala Ser Ser Arg Gly Glu Gly Glu Thr Ser Asp
100 105 110
Ser Cys Arg Asp Gly Glu Pro His Asp Pro Gly Val Gly Leu Pro Trp
115 120 125
Val Ser Ala Asn Phe Ser Gly Thr Tyr Gln Ala Ala Leu Arg Asp Ala
130 135 140
His Gln Ala Trp Gln Asn Phe Phe Asp Ser Arg Ser Gly Lys Arg Lys
145 150 155 160
Gly Arg Phe Ala Gly Arg Pro Lys Phe Lys Ser Lys Arg Asn Pro Lys
165 170 175
Arg Ser Phe Gln Thr His Gly Thr Gly Leu Arg Ile Ala Asp His Arg
180 185 190
His Val Val Leu Pro Lys Ile Gly Ser Val Arg Thr His Glu Pro Thr
195 200 205
Lys Lys Leu Leu Arg Arg Leu Arg Lys Gly Glu Thr Ala Cys Ala Thr
210 215 220
Cys Arg Thr Ser Gly Val Val Lys Val Glu Thr Lys Ala Gly Thr Lys
225 230 235 240
Glu Arg Lys Cys Gly Thr Cys Arg Gly Thr Gly Gln Ala Pro Tyr Ala
245 250 255
Arg Ile Val Arg Ala Thr Val Ser Leu His Thr Asn Asn Arg Trp Tyr
260 265 270
Ile Ser Ile Thr Val Glu Ala Gln Arg Glu Ile Arg Thr Gly Pro Ser
275 280 285
Gln Arg Gln Arg Glu Gly Gly Arg Ile Gly Leu Asp Leu Gly Val Arg
290 295 300
Ser Leu Leu Val Thr Ser Arg Gly Asp Arg Ile Ala Asn Pro Arg His
305 310 315 320
Leu Asp Lys Ala Leu Arg Arg Leu Arg His Ala Gln Gln Asp Leu Ser
325 330 335
Arg Thr Glu Ile Gly Ser Gly Arg Arg Glu Arg Ser Arg Lys Arg Val
340 345 350
Ala Gln Met His Ala Arg Val Gly His Leu Arg Ala Asp Thr Cys His
355 360 365
Lys Ile Thr Thr Asp Leu Val His Arg Tyr Ala Gly Ile Ala Val Glu
370 375 380
Gly Trp Asp Ile Gln Gln Thr Ala Glu Arg Gly Ser Arg His Leu Glu
385 390 395 400
Thr Arg Lys Arg Arg Glu Arg Asn Arg Asn Leu Ala Asp Ala Ala Leu
405 410 415
Gly Asn Leu Arg Trp Gln Leu Gln Ser Lys Gly Arg Trp Tyr Gly Cys
420 425 430
Glu Val Asp Val Thr Asp Pro His Ala Pro Thr Gly Arg Thr Cys Ser
435 440 445
Ala Cys Gly Ala Val Arg Thr Lys Pro Leu Pro Pro Ala Glu Glu Leu
450 455 460
Phe Met Cys Pro Ser Cys Gly Tyr Ala Val Asp Arg Arg Val Asn Thr
465 470 475 480
Ala Arg Ser Val Ala Arg Leu Ala Arg Glu Ser Lys Asn Gly Ala Ala
485 490 495
Gly Ser Gly Ser Glu Ala Lys Asn Ala Arg Gly Gly Gly Val Arg Pro
500 505 510
Gly Ala Ser Arg Arg Ala Gly Gln Ser Pro Ser Asn Arg Glu Ala Arg
515 520 525
Ala Gly Pro Lys Gly Pro Gly Lys Thr Gly Thr Pro Ala Gly
530 535 540
<210> 88
<211> 564
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 88
Met Pro Val Lys Asp Glu Leu Ile Thr Gln Ala Tyr Arg Tyr Ala Leu
1 5 10 15
Asp Pro Thr Pro Ala Gln Glu Arg Ala Phe Thr Ser His Val Gly Gly
20 25 30
Ala Arg Phe Ala Tyr Asn Trp Gly Leu Ala Thr Tyr Ala Ala Ala Leu
35 40 45
Asp Ala Arg Glu Ala Glu Lys Ala Ala Gly Glu Lys Pro Thr Thr Arg
50 55 60
Leu Pro Gly His Phe Glu Leu Cys Arg Leu Trp Thr Ala His Lys Asp
65 70 75 80
Thr Thr Glu Trp Val Asp Arg Asp Thr Gly Glu Thr Thr Thr Gly Ile
85 90 95
Pro Trp Val Ser Arg Asn Phe Ser Gly Thr Tyr Gln Ala Ala Leu Arg
100 105 110
Asp Val His Gly Ala Trp Lys Lys Phe Trp Asp Ser Arg Asn Gly Ser
115 120 125
Arg Ala Gly Arg Lys Met Gly Arg Pro Arg Phe Lys Ser Lys His Arg
130 135 140
Ser Arg Pro Ala Phe Gln Val His Gly Ser Gly Leu Gln Val Ala Asp
145 150 155 160
Ser His His Val Lys Leu Pro Thr Val Gly Thr Val Lys Thr Cys Glu
165 170 175
Ser Thr Arg Lys Leu Leu Arg Leu Leu Gln Thr Gly Gln Lys Thr Cys
180 185 190
Pro Asp Cys Ser Gly Gln Gly Arg Ile Arg Arg Gly Pro Lys Thr Ser
195 200 205
Arg Ser Gln Arg Arg Leu Phe His Gly Arg Pro Ala Trp Thr Ser Asp
210 215 220
Arg Phe Lys Thr Cys Gln Ser Cys Lys Gly Asn Gly Lys Leu Asp Pro
225 230 235 240
Thr Pro Asp Ala Arg Leu Gln Ala Cys Pro Thr Cys Lys Ala Thr Gly
245 250 255
Gln Val Pro Gly Lys Gly Ala Pro Lys Asn Cys Pro Asp Cys Lys Gly
260 265 270
His Thr Gln Val Pro Thr Ala Arg Ile Val Arg Gly Thr Ile Ser Gln
275 280 285
His Ser Asp Gly Arg Trp Tyr Leu Ala Leu Thr Val Gln Arg Ile Arg
290 295 300
Gln Val Arg Thr Ser Pro Ser Arg Ala Gln Arg Asn Gly Gly Thr Ile
305 310 315 320
Gly Ile Asp Leu Gly Val Arg Ser Leu Val Val Thr Ser Asp Gly Gln
325 330 335
Thr Val Asp His Pro Lys His Leu Thr Lys Ser Leu Gly Lys Leu Ala
340 345 350
Ala Ala Gln Lys Val Ala Ser Arg Arg Glu Lys Gly Ser Ala Arg Arg
355 360 365
Glu Lys Ala Asn Arg Lys Val Ala Arg Leu His Ala Arg Val Gly His
370 375 380
Leu Arg Ser Asp Ala Thr Gln Lys Leu Thr Ser His Leu Ile His His
385 390 395 400
His Gln Arg Ile Gly Val Glu Gly Trp Asp Val Gln Gln Thr Ala Gln
405 410 415
His Gly Ser Lys Asp Val Pro Lys Lys Val Arg Ala Lys Arg Asn Leu
420 425 430
Ala Leu Ala Asp Ala Asn Leu Gly Gln Ile Arg Trp Gln Leu Gln Ser
435 440 445
Lys Ala Val Trp Tyr Gly Thr His Val Val Val Ser Gly Lys His Thr
450 455 460
Ala Thr Gly Arg Thr Cys Ser Val Cys Ser Gln Glu Arg Ala Asn Pro
465 470 475 480
Val Pro Pro His His Asp Glu Phe Gln Cys Pro Glu Cys Gly Trp Val
485 490 495
Gly Asp Arg Arg Val Asn Thr Ala Arg Val Leu Ala Gln Leu Ala Gln
500 505 510
Gln Gln Gln Asn Asp Ala Ser Ser Ala Gly Glu Ser Leu Asn Ala Arg
515 520 525
Gly Ala Asp Val Ser Pro Ala Arg Pro Arg Pro Gly Gly Gln Leu Ala
530 535 540
Val Lys Arg Glu Ala Arg Ser Arg Ser Glu Gly Arg Gly Asp Pro Gly
545 550 555 560
Ser Pro Gly Pro
<210> 89
<211> 498
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 89
Met Pro Tyr Asp Ala Val Pro Asn Val Val Leu Arg Arg Ala Gln Gln
1 5 10 15
Ser Val His Asp Ala Tyr Leu Ser Tyr Leu Arg Leu Arg Arg Asn Gly
20 25 30
Ala Thr Glu Ala Lys Pro Pro Gly Phe Arg Ser Ser Arg Arg Ala Met
35 40 45
Pro Cys Leu Gly Tyr Gln Thr Gln Thr Arg Ser Arg Ala Gly Thr Val
50 55 60
Glu Asp Trp Leu Pro Gln Arg Gly Pro Lys Trp Ala Leu Val Arg Val
65 70 75 80
Pro Gln Pro Arg Ser Ser Val Ala Arg Ile Ser Asp Ala Arg Val Arg
85 90 95
Leu Arg Tyr His Arg Pro Ile Pro Asp Gly Ala Glu Val Gln Leu Ala
100 105 110
Thr Trp Ser Phe Asp Arg Ala Gly Gly His Trp Tyr Val Ser Leu Gln
115 120 125
Cys Ser Leu Ala Val Pro Ala Ser Glu Pro Ser Ser Thr Pro Val Val
130 135 140
Val Gly Leu Asp Arg Gly Val Arg Asp Leu Ala Ala Cys Val Gly Val
145 150 155 160
Asp Ser Ala Thr Arg Gln Ile Val Val Asp Arg Ala Val Gln Pro Ser
165 170 175
Pro Gly Leu Thr Pro Ala Arg Arg Arg Arg Leu Arg Leu Leu Glu Arg
180 185 190
Ser Val Ala Arg Ser His Arg Ala Val Cys Pro Gly Tyr Glu Pro Gly
195 200 205
Gln Arg His Pro Ser Gly Met Cys Met Trp Arg Arg Arg Lys Pro Ala
210 215 220
Arg Ala Arg Arg Ala Glu Ala Glu Ile Ala Arg Leu Arg Ala Ala Asp
225 230 235 240
Ala Arg Ala Arg His Glu Val Ile Glu Val Leu Thr His Asp Leu Val
245 250 255
Asp Gly Ser Pro Gln Arg Ser Trp Gln Pro Ala Asp Val Val Ala Val
260 265 270
Glu Ser Leu Arg Val Lys Asp Met Met Arg Ser Ala Arg Gly Thr Ala
275 280 285
Asp Asp Pro Gly Ala Asn Val Thr Ala Lys Ala Gly Leu Asn Arg Ser
290 295 300
Ile Gln Ala Ala Ser Trp Ser Arg Leu Ser Ile Arg Leu Cys Ala Lys
305 310 315 320
Ala Gly Arg Ala Arg Leu Val Gly Arg Thr Val Glu Val Val Glu Val
325 330 335
Asp Ala Arg Asn Thr Ser Arg Thr Cys Pro Glu Cys Asp Asp Val Asp
340 345 350
Pro Ala Asn Arg Arg His Gly Gly Lys Ser Phe Ala Cys Gly Arg Cys
355 360 365
Gly His Arg Asp Asp Ala Asp Arg Val Ala Ala Arg Asn Ile Val Thr
370 375 380
Arg Ala Leu Asp Leu Ile Glu Gly Arg Leu Ala Pro Ala Asp Gly Ala
385 390 395 400
Pro Ser Ala Gly Cys Gly Gly Leu Gly Val Ser Glu Leu Pro Pro Gly
405 410 415
Asp Gly Gly Ser Gly Pro Ala Leu Val Pro Arg Gly Glu Ser Ala Gly
420 425 430
Glu Ala Pro Leu Arg Ser Arg Arg Thr Ala His Ser Ala His Arg Ile
435 440 445
His Ala Ser Ala Pro Ala Ala Asp Asp Ala Gly Val Ser Ala Arg Ser
450 455 460
Gly Gln Ala Pro His His Glu Glu Val Arg Tyr Gly Asp Arg Asp Gly
465 470 475 480
Gln Leu Val Glu Val Ala Leu Ala Ser Gly Thr Val Met Ala Gly Gly
485 490 495
Ala Pro
<210> 90
<211> 538
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 90
Met Gln Ala Thr Glu Ser Pro Ala Gly Phe Pro Ser Ser Gln Glu Ala
1 5 10 15
Gln Ser Ser Ile Cys Arg Ala Tyr Arg Phe Ala Leu Asp Leu Ser Pro
20 25 30
Glu Gln Ile Gln Asp Met Gln Arg His Ala Gly Ala Ala Arg Trp Ala
35 40 45
Phe Asn Phe Ala Leu Glu Met Lys Val Ala Ala His Gln Arg Trp Arg
50 55 60
Ala Asp Val Glu Ala Leu Ile Ala Gly Gly Val Pro Glu Ala Asp Ala
65 70 75 80
Arg Lys Gln Val Lys Ala Pro Phe Pro Lys Val Lys Asp Ile Ala Ala
85 90 95
Tyr Trp Arg Thr Val Arg Gly Asp Ser Arg Gly Gly Asp Ala Ala Ala
100 105 110
Gln Thr Gly Arg Ser Pro Trp Trp His Glu Val Ser Ser Tyr Ala Ile
115 120 125
Ser Thr Gly Met Arg Asn Ala Glu Thr Ala Trp Ser Asn Trp Leu Lys
130 135 140
Ser Phe Lys Lys Gln Arg Lys Gly Ala Arg Val Gly Tyr Pro Arg Phe
145 150 155 160
Lys Ala Arg His Arg Ser Arg Asp Ser Phe Thr Ile Tyr His Asp Val
165 170 175
Arg Lys Pro Thr Ile Pro Ile Ala Asp Tyr Arg His Leu Gln Ile Pro
180 185 190
Arg Leu Gly Leu Val Arg Val His Asn Ala Gly Arg Arg Ile Gly Ala
195 200 205
Gly Lys Gly Gln Gln Arg Leu Gly Ser Thr Lys Arg Leu Val Arg Met
210 215 220
Ile Glu Arg Gly Glu Ala Thr Leu Lys Ser Val Thr Val Ser Arg Gly
225 230 235 240
Gly Thr Arg Trp Tyr Ala Ser Val Leu Leu Glu Ile Thr Glu Ala Gly
245 250 255
Met Arg Gln Arg Glu Ser Val Thr Arg Lys His Gln Gly Ala Pro Lys
260 265 270
Gln Leu Gln Arg Thr Arg Pro Glu Ile Gly Val Glu Trp Gly Val Arg
275 280 285
Thr Leu Ala Thr Leu Ser Asp Gly Thr Thr Phe Ala Asn Pro Arg Tyr
290 295 300
Gly Lys Arg Asn Glu Arg Arg Leu Arg Arg Ala Ser Gln Asn Leu Ser
305 310 315 320
Arg Lys Pro Tyr Ala Arg Gly Thr Pro Ser Ser Ala Asn Arg Gln Lys
325 330 335
Ala Val Asp Arg Leu Arg Lys Val His His Gln Thr Ala Glu Ala Arg
340 345 350
Thr Ser Gly Leu His Gln Leu Ser Val Arg Ile Ala Val Asn Tyr Pro
355 360 365
Trp Val Ala Met Arg Glu Leu Asn Val Lys Ser Met Thr Lys Ser Ala
370 375 380
Arg Gly Ser Gln Glu Glu Pro Gly Arg Leu Val Lys Val Lys Ala Arg
385 390 395 400
Phe Asn Arg Ala Ile Leu Asp Ala Ala Pro Tyr Glu Leu Arg Arg Gln
405 410 415
Ile Glu Tyr Lys Cys Asp Ala Phe Gly Ala Ser Phe Gly Leu Ala Pro
420 425 430
Gly Asp Leu Arg Ser Ser Gln Asp Cys Ser Glu Cys Gly Trp Arg Asp
435 440 445
Pro Ser Ile Pro Leu Glu Gln Val Ile Phe Arg Cys Gly Asn Asp Glu
450 455 460
Cys Gly Leu Val Leu Asp Arg Glu Leu Asn Ser Ala Arg Asn Ile Arg
465 470 475 480
Asn His Ala Val Val Ala Pro Asp Gly Gly Glu Thr Leu Asn Ala Cys
485 490 495
Gly Glu Asp Ile Arg Arg Pro Gly Asp Arg Ser Gln Ser Ser Met Lys
500 505 510
Gln Glu Asp Trp Pro Pro Pro Asp Gly Gln Pro Ser Arg Gly Ser Asp
515 520 525
Ser Pro Ala Leu Leu Ala Ser Pro Asp Leu
530 535
<210> 91
<211> 659
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 91
Met Ser Ala Gln Ala Pro Ala Phe Leu Glu Leu Glu Ala His Ala Arg
1 5 10 15
Val Arg Val Leu Phe Gly Cys Pro Val Asn Met Thr Val Trp Arg Val
20 25 30
Thr Gly Ser Leu Cys Pro Val Ala Ile Cys Gly Pro Ala Gln Arg Leu
35 40 45
Gly Ser Gly Gly Thr Pro Arg Val Ala Ala Lys Ser His Pro Ala Gly
50 55 60
Arg Gly His Gly Pro Ala Ala Val Thr Met Pro Arg Ala Glu Val Leu
65 70 75 80
Arg Ala Tyr Arg Phe Ala Leu Asp Pro Ser Gly Ala Glu Arg Ala Ala
85 90 95
Leu Ser Arg Tyr Ala Gly Ala Cys Arg Trp Ala Tyr Asn Tyr Ala Leu
100 105 110
Ala Arg Lys Met Arg Ala His Gln Ala Trp Ala Asp Arg Arg Ser Ala
115 120 125
Tyr Leu Ala Gly Gly Leu Ser Glu Ala Glu Ala Lys Glu Arg Ile Arg
130 135 140
Ala Asp Gly Ala Glu Leu Thr Asp Arg Ile Lys Val Trp Asp His His
145 150 155 160
Arg Lys Thr Leu Thr Leu Thr Val Ala Gly Lys Pro Pro Leu Pro Ala
165 170 175
Met Gln Cys Pro Ala Gly Gln Glu Ala Leu Val Arg Arg Leu Ala Ala
180 185 190
Ala Arg Ala Asp Ala Ala Gly Thr Gly Ser Glu Arg Glu Leu Leu Ala
195 200 205
Glu Gly Arg Ala Met Val Asn Ala Leu Lys Ala Gln Ala Phe Thr Ala
210 215 220
Gly Phe Arg Thr Pro Thr Ala Ile Asp Thr Ser Ala Leu Trp Arg Met
225 230 235 240
Asp Arg Asp Leu Pro Gln Gln Glu Gly Gly Ser Pro Trp Trp Arg Glu
245 250 255
Val Asn Val Tyr Cys Phe Thr Ser Gly Phe Asp Arg Ala Gln Ala Ala
260 265 270
Trp Lys Asn Trp Gln Glu Ser Leu Ala Gly Arg Arg Ala Gly Arg His
275 280 285
His Gly Tyr Pro Arg Phe Lys Lys Lys Gly His Thr Glu Ser Phe Thr
290 295 300
Leu Phe His Asp Val Lys Arg Pro Ile Ile Arg Leu Glu Ser Tyr Arg
305 310 315 320
Arg Leu Val Met Pro Gly Leu Gly Ser Ile Arg Ile His Asp Ser Gly
325 330 335
Lys Arg Leu Ala Arg Leu Val Glu Arg Gly Gln Ala Val Ile Gln Ser
340 345 350
Val Thr Val Thr Arg Gly Gly His Arg Trp Tyr Ala Ser Val Leu Ala
355 360 365
Lys Val Gln Gln Asp Val Pro Val Leu Trp Glu His Val His Asp Asp
370 375 380
Gly Thr Arg Thr Ser Tyr Leu Ser Arg Thr Gln Ala Glu Glu Ala Ala
385 390 395 400
Gly Ser Gly Gly His Val Glu Gln Ile Gly Arg Pro Thr Ala Arg Gln
405 410 415
Arg Ala Gly Gly Leu Val Gly Val Gly Leu Gly Ser His Tyr Leu Ala
420 425 430
Ala Leu Ser Ser Pro Leu Asp Pro Ala Asp Pro Ala Thr Ala Leu Val
435 440 445
Gln His Pro Arg Leu Leu Ala Asp Ser Leu Ala Lys Leu Ser Lys Ala
450 455 460
Gln Arg Ala Met Ser Arg Cys Gln Gln Gly Ser Arg Arg Trp Ser Lys
465 470 475 480
Ala Thr Ala Gly Val Ser Arg Ile His Gln Gln Ile Thr Val Arg Arg
485 490 495
Ala Ser Phe Leu His Gly Leu Ser Lys Lys Leu Ala Thr Gly Phe Thr
500 505 510
His Val Ala Ile Glu Asp Leu Asp Ile Thr Ala Leu Thr Thr Ser Ala
515 520 525
Lys Gly Thr Arg Asp Glu Pro Gly Lys Asn Val Lys Ala Gln Ala Arg
530 535 540
Phe Asn Arg His Leu Leu Asp Ala Gly Leu Gly Ser Leu Arg Lys Lys
545 550 555 560
Leu Ala Tyr Lys Thr Ala Trp Tyr Gly Ser Gln Leu Val Val Leu Asp
565 570 575
Gln Gly Glu Pro Val Thr Ala Thr Cys Ala Lys Cys Lys Glu Arg Asn
580 585 590
Pro Ser Ser Asp Pro Ser Cys Ser Thr Phe His Cys Pro Ser Cys Gly
595 600 605
Ala Ala Val His Arg His Glu Asn Ser Thr Ala Asn Ile Val Asp Ala
610 615 620
Ala His Arg Lys Leu Thr Thr Val Ala Ser Asp Arg Gly Glu Thr Gln
625 630 635 640
Asn Ala Arg Arg Ala Thr Ala Ser Pro Gly Ala Arg Lys Ala Pro Gly
645 650 655
Lys Gly His
<210> 92
<211> 526
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 92
Met Ser Ala Gln His Asp Asp Asp Gly Gln Pro Leu Ala Ala Thr Pro
1 5 10 15
Ser Pro Ala Pro Ala Pro Thr Leu Ala Pro Thr Ala Val Pro Leu Pro
20 25 30
Val Pro Thr Pro Gly Pro Gly Pro Val Val Arg Ala Tyr Arg Leu Ala
35 40 45
Leu Asp Pro Thr Pro Thr Gln Ala Thr Ala Phe Ala Arg His Ala Gly
50 55 60
Ala Ser Arg Trp Ala Phe Asn Tyr Ala Leu Ala Ala Lys Lys Ala Ala
65 70 75 80
His Ala Ala Cys Arg Glu Gln Val Asp Ala Leu Thr Ala Ala Gly Val
85 90 95
Pro Glu Ala Glu Ala Arg Lys Ala Val Lys Val Glu Val Pro Gly Ala
100 105 110
Phe Thr Val Gln Lys Thr Trp Gln His Glu Arg Thr Glu Val Cys Pro
115 120 125
Trp Trp Gly Glu Val Ser Ser Lys Ala Phe Val Gln Ala Phe Arg Asp
130 135 140
Ala Asp Lys Ala Trp Thr Asn Trp Leu Ala Ser Leu Lys Gly Gln Arg
145 150 155 160
Ala Gly His Arg Val Gly Tyr Pro Arg Phe Lys Lys Arg Gly Arg Cys
165 170 175
Arg Asp Ser Phe Arg Leu Glu Gly Ala Ile Arg Pro Glu Gly Tyr Arg
180 185 190
His Leu Leu Leu Pro Arg Ile Gly Pro Val Arg Thr His Gln Thr Cys
195 200 205
Lys Pro Leu Ala Arg Ala Leu Glu Arg Gln Gly Gly Arg Ile Thr Ser
210 215 220
Val Thr Val Ser Arg Gly Ala His Arg Trp Tyr Ala Ser Val Val Val
225 230 235 240
Thr Phe Pro Ser Gly Thr Leu Pro Thr Pro Gln Arg Ala Ala Pro Thr
245 250 255
Arg Ala Gln Gln Arg Ala Gly Val Val Gly Val Asp Leu Gly Val Lys
260 265 270
His Leu Ala Ala Leu Ser Ala Leu Gly Ala Asp Gly Thr Leu Ala Glu
275 280 285
Ser Leu Val Ala Asn Pro Arg His Gly Ala Arg Gly Ala Arg Arg Leu
290 295 300
Ala Lys Ala Gln Arg Ala Leu Ser Arg Thr Gln Arg Gly Ser His Arg
305 310 315 320
Arg Ala Lys Ala Ala Ala Ile Val Ala Arg Arg His Ala Asp Leu Ala
325 330 335
Ala Gln Arg Ala Thr Ser Ile His Val Leu Thr Lys His Leu Ala Thr
340 345 350
Thr Tyr Ala Gly Val Ala Ile Glu Asp Leu His Val Ser Gly Met Thr
355 360 365
Arg Ser Ala Lys Gly Ser Leu Ala Lys Pro Gly Arg Asn Val Arg Ala
370 375 380
Lys Ala Gly Leu Asn Arg Ser Leu Ala Asp Val Ser Phe Gly Glu Ile
385 390 395 400
Arg Arg Gln Leu Ala Tyr Lys Thr Arg Trp Tyr Gly Ser Thr Leu Arg
405 410 415
Val Val Gly Arg Phe Glu Pro Thr Ser Lys Thr Cys Ser Ala Cys Gly
420 425 430
Arg Gln Asp Pro His Leu Ser Leu Ala Glu Arg Ile Tyr Glu Cys Pro
435 440 445
Cys Gly Leu Arg Leu Asp Arg Asp Leu Asn Ala Ala Arg Asn Ile Arg
450 455 460
Val Ala Gly Phe Glu Pro Ala Ala Trp Ala Ala Ala Val Ala Arg Gly
465 470 475 480
Ser Arg Glu Thr Ser Asn Ala Arg Arg Gly Pro Ala Ser Pro His Thr
485 490 495
Arg Pro Gly Val Gly Leu Gly Pro Leu Thr Arg Glu Asp His Pro Asp
500 505 510
Thr Ala Ala Ala Val Ala Val Gly Val Val Thr Ser Gly Arg
515 520 525
<210> 93
<211> 549
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 93
Met Ser Asp Gln Gln Pro Gln Pro Asp Ala Thr Asp Ser Pro Thr Thr
1 5 10 15
Ile Val Val Gln Arg Ala Trp Arg Cys Thr Leu Ala Pro Thr Pro Ala
20 25 30
Gln Gln Gln Met Leu Cys Gln His Ala Gly Ala Ala Arg Trp Ala Phe
35 40 45
Asn Leu Ala Leu Ser Ala Lys Ile Val Ser Arg Gly Val Trp Glu Gln
50 55 60
Arg Val Gln Ala Leu Val Asp Glu Glu Gly Val Pro Glu Asp Gln Ala
65 70 75 80
Arg Lys Gln Val Lys Val Lys Thr Pro Gly Ser Asn Val Leu Asp Lys
85 90 95
Ala Arg Val Arg Val Arg Gly Thr Asp Arg Trp Gly Glu Arg Cys Thr
100 105 110
Ala Asp Trp Val Asp Ala Arg Arg Val Leu Thr Asp Asn Lys Ile Asp
115 120 125
Glu Glu Val Ala Asp Glu Ile Leu Thr Arg Trp Ala Ser Arg Val Asp
130 135 140
Asp Pro Ser Glu Gly Ile Ala Pro Trp Leu Ala Glu Val Pro Asn Ser
145 150 155 160
Leu Val Gln Arg Gly Glu Lys Asn Ala Asp Lys Ala Trp Gln Thr Trp
165 170 175
Met Asp Ser Val Thr Gly Arg Arg Lys Gly Arg Arg Val Gly Phe Pro
180 185 190
Arg Phe Lys Lys Arg Gly Val Arg Asp Ser Phe Tyr Leu Thr Asn Thr
195 200 205
Glu Ser Gly Pro Cys Leu Gly Ser Asn Arg His Val Arg Leu Gly Gly
210 215 220
Lys Leu Gly Thr Val Arg Val Cys Asp Ala Asp Gly Lys Arg Ala Leu
225 230 235 240
Arg Arg Leu His Arg Arg Leu Arg Gln Gly Thr Cys Arg Ile Met Ser
245 250 255
Val Thr Val Ser Arg Gly Gly His Arg Trp Tyr Ala Ser Val Met Thr
260 265 270
Glu Glu Thr Leu Pro Ala Pro Arg Pro Ser Arg Arg Ala Val Glu Ala
275 280 285
Gly Thr Ile Gly Val His Leu Gly Val His Asp Arg Ala Val Leu Ser
290 295 300
Thr Gly Glu Ile Ile Glu Asn Pro Arg Ile Lys Ala Arg His Ala Arg
305 310 315 320
Ala Ile Ala Lys Ala Gln Arg Ala Leu Ala Arg Thr Gly Trp Thr Val
325 330 335
Asn Asp Asp Gly Arg Lys Arg Pro Thr Arg Arg Arg Glu Lys Ala Arg
340 345 350
Gln Arg Val Ala Arg Leu Ala His Leu Glu Ala Gln Arg Arg Asn Thr
355 360 365
His Thr His His Leu Ser Lys Arg Leu Ala Ala Glu Tyr Ala Glu Ile
370 375 380
Val Met Leu Asp Leu Asp Ile Gln Gly Met Thr Arg Thr Ala Arg Arg
385 390 395 400
Cys Arg Pro Pro Leu His Gly Trp Asp Ala Gln Ala Lys Arg Thr Ala
405 410 415
Asn Thr Ala Phe Leu Asp Val Ala Pro Phe Glu Leu Arg Arg Gln Val
420 425 430
Ala Tyr Lys Thr Thr Trp Arg Gly Ala Arg Leu Thr Val Ala Pro Lys
435 440 445
Gly Thr Ala Val Val Gly Ile Cys Pro Glu Cys Gly Ala Val Arg Ala
450 455 460
Lys Arg Pro Val Arg Gly Asp Arg Ile Thr Cys Pro Cys Gly Ala Val
465 470 475 480
Tyr Gly Arg Gly His Ala Ala Ala Leu Ile Leu Ala Arg His Gly Ala
485 490 495
Val Ala Cys Asn Thr Arg Glu Thr Leu Asn Ala Arg Gly Glu Asp Val
500 505 510
Arg Pro Thr Ala Pro Arg Asp Ser Gly Arg Ser Pro Val Lys Arg Glu
515 520 525
Gly Pro Arg His Leu Arg Gly Ser Pro Leu Gly Ser Asp Ala Glu Ala
530 535 540
Phe Pro Leu Arg Thr
545
<210> 94
<211> 533
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 94
Met Ser Asp Ser Val Ile Thr Arg Ala Tyr Lys Phe Arg Leu Cys Pro
1 5 10 15
Ser Phe Glu Gln Arg Val Arg Leu Trp Gly Cys Ala Glu Met Ala Arg
20 25 30
Met Ala Trp Asn Trp Thr Val Ala Leu Cys Glu Arg Glu Tyr Glu Glu
35 40 45
Ala Leu Ile Ala Ala Gly Val Leu Glu Gln Arg Glu Leu Asp Gly Cys
50 55 60
Glu Arg Arg Lys Gln Arg Val Ala Val Val Ala Arg Ala Arg Ala Arg
65 70 75 80
Ala Arg Glu Asn Lys Val Val Val Gln Ser Pro Ser Ile Asn Arg Gly
85 90 95
Arg Leu Tyr Lys Glu Trp Cys Val Met Arg Asp Ser Thr Ala Asn Ser
100 105 110
Ile Arg Asn Tyr His Ser His Thr Tyr Ser Tyr Pro Ile Ala Arg Val
115 120 125
Val Gln Ala Tyr Lys Asp Trp Trp Arg Lys Lys Gly Gly Arg Ala Gly
130 135 140
Ala Pro Arg Tyr His Arg Asp Ser His Pro Ser Ser Phe Thr Ile Gln
145 150 155 160
Leu Ser Arg Thr Val Trp Asp Asp Asp Gly Ile Arg Ile Pro Gly Val
165 170 175
Gly Tyr Val Arg Thr Thr Trp Arg Asn Arg Gly Arg Trp Cys Gly Glu
180 185 190
Ser Pro Ser Ala Ile Pro Pro Gly Lys Pro Cys Ser Ile Thr Val Thr
195 200 205
Arg Lys Ala Asn Ala Trp Tyr Ala Ser Ile Ala Met Lys Asp Val Gly
210 215 220
Phe Asp Glu Thr Ala Gly Thr Gly Val Val Gly Leu Asp Leu Gly Val
225 230 235 240
Ser Ser Val Leu Thr Thr Ser Asp Gly Gln Ser Trp Ala Pro Pro Arg
245 250 255
Pro Leu Glu Lys Ala Leu Arg Arg Val Ala Ile Leu Gln Arg Arg Arg
260 265 270
Gln Arg Lys Pro Lys Arg Ser Arg Arg Arg Thr Leu Leu Asp Ile Gln
275 280 285
Ile Ala Ser Leu His Ala Arg Val Ala Ala Ile Arg Tyr Asp His Leu
290 295 300
His Asn Val Ser Thr His Leu Ala Lys Thr Phe Gly Val Val Val Val
305 310 315 320
Glu Gly Phe Asp Val Val Gly Leu Val Ser Asn Glu Val Gln Trp Arg
325 330 335
Ala Ile Arg Arg Ala Met Met Asp Ile Ala Trp Gly Glu Leu Arg Arg
340 345 350
Gln Leu Glu Tyr Lys Leu Gln Trp Arg Gly Gly Thr Leu Met Thr Met
355 360 365
Gly Val Phe Asp Pro Thr Asn Gln Gln Cys Ala Ser Cys Trp Lys Thr
370 375 380
Gly Thr Val Thr Met Asn Ala Cys Pro Pro Ser Gln Ala Ile Tyr Arg
385 390 395 400
Cys Ser Thr Cys Asp Ala Lys Val Ser Arg Gln Met Asn Thr Ala Lys
405 410 415
Leu Leu Glu Leu Phe Gly Arg Phe Gly Pro Pro Pro Gln Gln Gly Thr
420 425 430
Gly Ala Gln Pro Gly Pro Arg Pro Pro Arg Arg Arg Gly Lys Gly His
435 440 445
Ala Arg Gly Gly Ala Glu Ser Thr Ala Cys Ser Ala Ala Pro Val Lys
450 455 460
Arg Glu Gln Thr Leu His Ala Pro Pro Gln Gly Ala Ser Pro Gly Ala
465 470 475 480
Arg Ala Leu Leu Ala Arg Thr Val Ser Asp Gly Cys Thr Gln Asp Gly
485 490 495
Gly Ser Ala Thr Pro Asn Pro Ser Cys Asn Lys Pro Gln Arg Lys Pro
500 505 510
Leu Val Arg Ala Arg Glu Gln Gly Asn Ser Glu Pro Val Ala Ala Glu
515 520 525
Val Gln Glu Gly Val
530
<210> 95
<211> 436
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 95
Met Ser Glu Gln Thr Arg Phe His Ala Phe Arg Tyr Thr Leu Ala Pro
1 5 10 15
Thr Pro Ser Gln Glu Ala Ala Phe Phe Arg Tyr Ala Gly Ala Ala Leu
20 25 30
Trp Ala Tyr Asn His Ala Ile Gly Val Lys Lys Ala Ala His Gln Glu
35 40 45
Trp Arg Thr Gln Val Asp Ala Leu Val Ala Ser Gly Ile Pro Glu Glu
50 55 60
Gln Ala Arg Lys Gln Val Lys Val Lys Ile Pro Asn Ser Phe Glu Ile
65 70 75 80
Gly Lys Asn Phe Asn Arg Ile Lys Gly Asp Ser Arg Glu Gly Ile Asp
85 90 95
Gly Ile Cys Pro Trp Trp His Glu Val Asn Arg Tyr Cys Phe Lys Ser
100 105 110
Ala Phe Ala Asp Ala Asp Gln Ala Trp Lys Asn Trp Leu Ala Ser Leu
115 120 125
Lys Gly Thr Arg Lys Gly Arg Lys Val Gly Tyr Pro Arg Phe Lys Ser
130 135 140
Lys Arg His Ser Arg Arg Ser Phe Arg Leu Tyr Glu Gly Ile Arg Pro
145 150 155 160
Val Gly Tyr Arg Arg Leu Arg Leu Pro Ser Leu Gly Glu Ile Arg Ile
165 170 175
His Gly Thr Thr Lys Pro Leu Val Lys Leu Leu Asn Ser Gly Gln Ala
180 185 190
Thr Ile Lys Ala Val Thr Val Ser Gln Gly Gly His Arg Trp Tyr Ala
195 200 205
Ser Val Leu Cys Glu Val Thr Thr Asn Leu Pro Asp Gly Pro Thr Arg
210 215 220
Arg Gln Arg Ser Arg Gly Thr Ile Gly Val Asp Met Gly Val Lys Asn
225 230 235 240
Leu Ala Thr Leu Ser Lys Pro Leu Asn Pro Asp Asp Pro Thr Thr Ala
245 250 255
Ile Val Pro Asn Pro Arg His Leu Glu Ala Thr Asp Arg Arg Leu Arg
260 265 270
Arg Ala Gln Arg Ala Leu Ala Arg Lys Gln Lys Gly Ser Lys Arg Arg
275 280 285
Glu Lys Ala Ala Arg Arg Val Ala Arg Leu His His Gln Val Ala Val
290 295 300
Arg Arg Ala Thr Ala Ile His Glu Leu Thr Lys Gln Leu Ala Thr Ser
305 310 315 320
Phe Glu Ala Val Ala Ile Glu Asp Leu Asn Val Gln Gly Met Thr Arg
325 330 335
Thr Ala Lys Gly Thr Ile Asp Arg Pro Gly Arg Asn Val Lys Ala Lys
340 345 350
Ala Gly Leu Asn Arg Ala Ile Leu Asp Ala Gly Phe Gly Glu Leu Arg
355 360 365
Arg Gln Leu Glu Tyr Lys Thr Thr Trp Tyr Gly Ser Arg Leu Leu Thr
370 375 380
Val Asp Arg Trp Met Pro Ser Ser Lys Thr Cys Ser Gly Cys Gly Trp
385 390 395 400
Lys Ala Pro Glu Leu Leu Leu Gln Thr Arg Val Phe Arg Cys Gly Gly
405 410 415
Cys Gly Leu Val Leu Asp Arg Asp Val Asn Ala Ala Arg Asn Ile Glu
420 425 430
Arg His Ala Ala
435
<210> 96
<211> 483
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 96
Met Ser Gly Val Gly Ala Thr Val Leu Arg Val Val Asp Pro Gly Ser
1 5 10 15
Thr Val Val Asp Glu Asn Glu Arg Arg Arg Gln Arg Ala Tyr Arg Phe
20 25 30
Ala Leu Asp Pro Thr Pro Ala Gln Glu Ala Val Leu Thr Ala His Ala
35 40 45
Asp Ala Ala Arg Trp Ala Phe Asn Phe Ala Leu Ala Gln Lys Lys Ala
50 55 60
Ala Asn Glu Thr Trp Arg Leu Gln Val Ala Glu Leu Thr Gly Leu Gly
65 70 75 80
Phe Thr Glu Ala Glu Ala Arg Lys Thr Ala Val Lys Thr Pro Asn Ala
85 90 95
Pro Glu Val Ala Arg Arg Trp Thr Ala Trp Arg Asp Asn Pro Ala Ala
100 105 110
Asp Pro Trp Arg Gly Gly Ile Cys Pro Trp Trp Arg Gly Ile Asn Arg
115 120 125
Phe Ala Phe Ser Ser Gly Phe Arg Asp Ala Asp Gln Ala Trp Ser Asn
130 135 140
Trp Met Ser Ser Leu Ala Gly Lys Arg Ala Gly Arg Arg Val Gly Glu
145 150 155 160
Pro Arg Phe Lys Arg Lys Gly Arg Val Thr Pro Ser Phe Arg Leu His
165 170 175
His Asp Val Lys Lys Pro Thr Ile Arg Thr Asp Gly Tyr Arg Arg Leu
180 185 190
Arg Met Pro Asn Ile Gly Thr Val Arg Leu His Asp Thr Ala Lys Arg
195 200 205
Met Val Arg Ala Leu Glu Arg Gly Gly Arg Val Gln Ser Val Thr Cys
210 215 220
Ser Arg Arg Gly His Arg Trp Tyr Ala Ser Val Leu Val Asp Glu Pro
225 230 235 240
Asp Val Thr Pro Gly Arg Glu Thr Gln Val Gly Pro Ser His Arg Gln
245 250 255
Lys Asn Ala Gly Arg Ile Gly Val Asp Leu Gly Thr Lys Ala Leu Ala
260 265 270
Ala Leu Ser Glu Pro Val Pro Gly Leu Ser Asp Glu His Gly Leu Ile
275 280 285
Glu Asn Pro Arg His Leu Lys Lys Ala Arg Arg Arg Leu Val Arg Ala
290 295 300
Ala Arg Gln Met Ser Arg Arg Val Gly Pro Asp Lys Arg Thr Gly Gln
305 310 315 320
Lys Pro Ser Asn Arg Trp Lys Arg Ala Arg Ala Arg Leu Ser Lys Ile
325 330 335
Gln Arg Glu Val Thr Val Gln Arg Ala Ser His Leu His Trp Ile Thr
340 345 350
Lys Thr Leu Ser Thr Ser Tyr Ala Glu Val Ala Ile Glu Asp Leu Asn
355 360 365
Val Ala Gly Met Thr Arg Ser Ala Lys Gly Asp Val Ala Glu Pro Gly
370 375 380
Arg Asn Val Arg Ala Lys Thr Gly Leu Asn Arg Ser Ile Leu Asp Ala
385 390 395 400
Ala Pro Gly Glu Phe Arg Arg Gln Leu Asp Tyr Lys Thr Pro Trp Tyr
405 410 415
Gly Ser Thr Val Ala Val Cys Asp Arg Trp Ala Pro Thr Ser Lys Thr
420 425 430
Cys Ser Ala Cys Gly Ala Ala Lys Thr Lys Leu Pro Leu Ser Glu Arg
435 440 445
Val Tyr Thr Cys Asn His Cys Gly Phe Val Cys Asp Arg Asp Val Asn
450 455 460
Ala Ala Arg Asn Ile Ala Ala Met Ala Val Pro Val Asp Pro Gly Gly
465 470 475 480
Gly Arg Arg
<210> 97
<211> 578
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 97
Met Ser Lys Gln Val Ile Pro Ile Ala Arg Gly Arg Gly Gly Lys Ala
1 5 10 15
Met Ala Glu Lys Thr Ser Thr Asp Asp Gly Ala Leu Tyr Arg Ala Tyr
20 25 30
Lys Phe Arg Leu Asp Pro Asn Gln Ala Gln Lys Ala Glu Leu Met Arg
35 40 45
Cys Val Gly Ala Ala Arg Cys Thr Phe Asn Leu Leu Asn Glu Tyr Asn
50 55 60
Leu Gln Ile Leu Arg Asn Glu Gln Glu Tyr Arg Arg Thr Arg Ala Ala
65 70 75 80
Glu Gly Ile Asp Tyr Gly Thr Ile Asn Gly Glu Leu Lys Lys Leu Gly
85 90 95
Lys Lys Asp Pro Glu Tyr Lys Phe Leu Gly Gln Ala Glu Tyr Glu Lys
100 105 110
Arg Tyr Leu Thr Pro Glu Lys Gln Arg His Glu Ala Ile Ala Gln Ala
115 120 125
Ile Ala Asp Gly Ala Asp Pro Ala Ile Val Trp Pro Glu Thr Glu Arg
130 135 140
Phe Thr Glu Pro Trp Leu His Thr Ile Ala Arg Arg Val Leu Val Ser
145 150 155 160
Gly Ile Lys Asn Ala Asp Lys Ala Trp Asp Asn Tyr Asn Lys Ser Arg
165 170 175
Met Lys Gln Arg Ala Gly Ala Arg Met Gly Val Pro Arg Arg Lys Arg
180 185 190
Lys Gly Ile Ser His Asp Ser Phe Thr Val Asp Arg Asp Pro Arg Lys
195 200 205
Asp Glu Val Gly Ala Tyr Gly Thr Pro Tyr Lys Gln Gly Glu Pro Glu
210 215 220
Tyr Val Arg Arg Lys Ala Gln Leu Lys Arg Arg Ser Ile Lys Thr Thr
225 230 235 240
Pro Thr Ile Gly Asp Tyr Arg His Val Arg Leu Ser His Leu Gly Val
245 250 255
Ile Arg Thr His Asn Thr Thr Lys Pro Leu Val Lys Ala Val Arg Ala
260 265 270
Gly Ala Asn Val Lys Ser Tyr Thr Val Ser Arg Ala Ala Asn Arg Trp
275 280 285
Tyr Val Ser Ile Leu Val Glu Leu Thr Arg Pro Ser Ala Thr Pro Thr
290 295 300
Arg Ala Gln Arg Ala Ala Gly Ala Val Gly Val Asp Leu Gly Val Arg
305 310 315 320
Tyr Leu Ala Ala Leu Ser Asp Glu Gln Ala Pro Gln Arg Phe Ala Arg
325 330 335
Tyr Pro Ser Leu Glu Phe Thr Gly Asp Gly Val Pro Thr Leu Ala Asn
340 345 350
Pro Arg Trp Ala Arg Thr Ala Glu Arg Arg Leu Val Arg Leu Gln Arg
355 360 365
Ala Leu Ala Arg Ala Gln Lys Gly Ser Asn Arg Arg Ala Arg Ile Val
370 375 380
Lys Gln Ile Ala Arg His His His Leu Val Ala Leu Arg Arg Glu Ser
385 390 395 400
Gly Leu His Gln Val Ser Lys Arg Leu Thr Thr Gly Tyr Thr Leu Ile
405 410 415
Gly Leu Glu Asp Leu Ala Val Ala Gly Met Thr Val Ser Ala Ala Gly
420 425 430
Thr Ile Glu Ala Pro Gly Lys Asn Val Arg Gln Lys Ala Gly Leu Asn
435 440 445
Arg Ser Ile Leu Asp Ala Ala Phe Ser Thr Leu Arg Arg Gln Leu Glu
450 455 460
Tyr Lys Ala Ser Trp Tyr Gly Ser Arg Val Gln Ile Ile Asp Arg Phe
465 470 475 480
Phe Ala Ser Ser Gln Thr Cys Ser Ala Cys Gly Ala Arg Ala Lys Thr
485 490 495
Lys Leu Asp Leu Arg Val Arg Val Phe Glu Cys Ala Ala Cys Gly Val
500 505 510
Arg Ile Asp Arg Asp Val Asn Ala Ala Arg Asn Ile Cys Ala Glu Ala
515 520 525
Val Arg Met His Glu Ala Gln Leu Ala Pro Gly Thr Gly Glu Ser Leu
530 535 540
Asn Gly Arg Gly Val Ala Asp Ser Asp Thr Thr Gly Ser Val Val Leu
545 550 555 560
Glu Asp Gly Ala Leu Asp Ala Ser Arg Pro Ala Ala Thr Gly Gly Gly
565 570 575
Ser Pro
<210> 98
<211> 519
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 98
Met Ser Gln Asp Arg Thr Arg Gly Phe Val Val Lys Leu Ala Pro Thr
1 5 10 15
Trp Glu Gln His Arg Leu Met Leu Ser Val Ala Arg Ala Arg Arg Arg
20 25 30
Val Lys Asn Phe Leu Val Leu Glu Ala Arg Arg Arg Leu Ser Val Ser
35 40 45
Ile Trp Gln Ser Cys Val Ala Tyr Ala Ala Thr Lys Gly Leu Glu Ala
50 55 60
Ala Glu Val Arg Arg Gln Trp Ala Asp Lys Lys Glu Gly Arg Pro Ala
65 70 75 80
Leu Arg Ala Ala Gly Phe Lys Ile Thr Pro Pro Ala Ala Leu Asp Tyr
85 90 95
Arg Ala Thr Thr Lys Trp Ala Gly Val Arg Ser Arg Arg Trp Met Leu
100 105 110
Ser Val Ser Ser Glu Ala Arg Lys Phe Ala Cys Asn Ser Phe Arg Thr
115 120 125
Ala Gln Gln Asn Tyr Val Ser Ser Ile Met Gly Arg Arg Lys Gly Ala
130 135 140
Pro Leu Gly Pro Pro Ala Val Glu Ser Arg Phe Ala Lys Pro Thr Trp
145 150 155 160
Thr Tyr Gln Val Pro Pro Ser Arg Trp Arg Ala Trp Val Thr Ser Thr
165 170 175
His Ile Trp Ile Pro Gly Val Gly Ala Val Arg Val Ser Glu Asp Pro
180 185 190
Leu Lys Gly Ala His Gly Gly Glu Val Ala Leu Val Ser Val Lys Arg
195 200 205
Asp Gly Ser Arg Trp Tyr Ala Val Ile His Tyr Arg Lys Met Arg Asp
210 215 220
Val Glu Arg Pro Phe Gly Arg Asn Ser Gly Ser Val Gly Val Asp Met
225 230 235 240
Asn Val Gly Asp Asn Gly Ile Val Leu Ser Thr Gly Lys Arg Tyr Pro
245 250 255
Val Pro Arg Ser Leu Glu Val Leu Asp Ala Lys Ile Arg Lys His Gln
260 265 270
Arg Ile Asn Arg His Lys Ala Gly Pro Arg Pro Gly Lys Ser Pro Ser
275 280 285
Val Arg Trp Arg Lys Gln Asn Arg Arg Leu Arg Asp Leu His Arg Glu
290 295 300
Val Val Arg Val Arg Thr Asp Trp Leu His Lys Val Thr Ser Glu Ile
305 310 315 320
Val Lys Glu Phe Arg Val Val Val Val Glu Gly Phe Ser Val Lys Ile
325 330 335
Leu Thr Ala Ser Ala Arg Gly Thr Val Glu Ala Pro Gly Val Asn Val
340 345 350
Arg Gln Lys Ala Gly Leu Asn Arg Lys Ile Leu Gln Ala Gly Phe Gly
355 360 365
Ser Phe Arg Glu Met Leu Lys Tyr Lys Thr Gly Ala Ile Gly Gly Val
370 375 380
Phe Val Met Ile Asp Arg Phe Phe Pro Ser Ser Lys Leu Cys Ser Arg
385 390 395 400
Cys Gly Trp Lys Asp Val Asp Gln Thr Leu Arg Asp Arg Val Phe Arg
405 410 415
Cys Arg Glu Cys Gly Leu Thr Ile Asp Arg Asp His Asn Ala Ala Arg
420 425 430
Asn Ile Arg Arg Ala Tyr Glu Asp Ser Val Val Asp Ala Ser Thr Thr
435 440 445
Glu Ser Thr Gly Gly Glu His Arg Pro Trp Thr Gly Gln Pro Leu Ile
450 455 460
Glu Gly His Val Leu Thr Ile Lys Ser Pro Gly Lys Thr Pro Ala Arg
465 470 475 480
Glu Gln Lys Arg Lys Arg Thr Lys Asp Val Arg Gly Arg Ser Ala Asp
485 490 495
Leu Gly Gln Ala Gly Gly Ala His Gly Pro Lys Thr Ser Ser Asp Ala
500 505 510
Arg Gly Asp Asp Ala Pro Val
515
<210> 99
<211> 440
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 99
Met Ser Arg Gln Ile Ile Leu Arg Ala Tyr Arg Tyr Ala Leu Asp Pro
1 5 10 15
Thr Pro Ala Gln Ser Gly Asp Leu Asn Arg His Ala Gly Ala Ala Arg
20 25 30
Trp Ala Tyr Asn His Ala Leu Ala Val Lys Val Ala Ala His Glu Glu
35 40 45
Trp Arg Arg Arg Val Ala Gly Leu Val Glu Ala Gly Thr Pro Glu Val
50 55 60
Glu Ala Arg Lys Gln Val Lys Val Pro Val Pro Ala Lys Pro Ala Ile
65 70 75 80
Gln Lys Ala Leu Asn Gln Val Lys Gly Asp Ser Arg Thr Gly Val Asp
85 90 95
Gly Val Cys Pro Trp Trp His Glu Val Asn Thr Tyr Ala Phe Gln Ser
100 105 110
Ala Phe Leu Asp Ala Asp Thr Ala Trp Lys Asn Trp Met Asp Ser Leu
115 120 125
Lys Gly Arg Arg Lys Gly Arg Pro Val Gly Tyr Pro Arg Phe Lys Lys
130 135 140
Arg Gly Ala Val Ala Ser Phe Arg Leu His His Asp Val Lys Arg Pro
145 150 155 160
Ser Ile Arg Leu Ala Gly Tyr Arg Arg Leu Arg Leu Pro Arg Ile Gly
165 170 175
Glu Val Arg Ile His Gly Ser Gly Lys Asp Leu Ala Arg Ala Val Ala
180 185 190
Arg Gly Asp Ala Val Val Gln Ser Val Thr Val Ser Arg Arg Gly His
195 200 205
Arg Trp Tyr Ala Ser Val Leu Cys Lys Val Thr Val Glu Ile Pro Asp
210 215 220
Gly Pro Thr Arg Ala Gln Arg Ala Arg Gly Ala Val Gly Val Asp Ala
225 230 235 240
Gly Val Lys Val Leu Ala Ala Leu Ser Lys Pro Leu Asp Ala Ala Asp
245 250 255
Pro Gly Thr Val Ile Val Pro Asn Gly Arg Pro Leu Ala Val Ala Ser
260 265 270
Arg Arg Leu Val Lys Ala Gln Arg Ala Leu Ala Arg Lys Gln Lys Gly
275 280 285
Ser Asn Arg Arg Val Lys Ala Val Arg Arg Val Ala Arg Leu His His
290 295 300
Glu Val Ala Leu Arg Arg Gly Thr Ala Ile His Thr Leu Thr Lys Arg
305 310 315 320
Leu Ala Ala Gly Phe Asp Thr Val Ala Val Glu Asp Leu Asn Val Ala
325 330 335
Gly Met Val Arg Ser Ala Lys Gly Thr Val Glu Gln Pro Gly Arg Asn
340 345 350
Val Arg Ala Lys Ala Gly Leu Asn Arg Ser Val Leu Asp Ala Ala Leu
355 360 365
Gly Glu Met Arg Arg Gln Leu Glu Tyr Lys Thr Gly Trp Tyr Gly Ser
370 375 380
Arg Leu Ala Val Val Asp Arg Trp Phe Pro Ser Ser Lys Thr Cys Ser
385 390 395 400
Val Cys Gly Ala Val Lys Glu Ser Leu Ala Leu Ser Glu Arg Val Tyr
405 410 415
Val Cys Ser Val Cys Gly Ala Arg Leu Asp Arg Asp Val Asn Ala Ala
420 425 430
Arg Asn Ile Glu Ala Ala Gly Ile
435 440
<210> 100
<211> 495
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 100
Met Ser Thr Gly Asp Val Glu Leu Arg Ala Tyr Arg Phe Ala Leu Asp
1 5 10 15
Leu Thr Pro Ala Gln Glu Gln Gln Ile Arg Gln His Ala Gly Ala Ala
20 25 30
Arg Trp Ala Tyr Asn His Ala Leu Ala Ala Lys Phe Ala Ala Leu Asp
35 40 45
Gln Arg Gln Ala Thr Ile Ala Ala Leu Val Glu Gln Gly Val Asp Pro
50 55 60
Lys Ala Ala Ala Ala Gln Ala Pro Lys Ile Pro Thr Lys Pro Ala Ile
65 70 75 80
Gln Lys Ala Leu Asn Glu Ala Lys Gly Asp Asp Arg Thr Gly Ala Asp
85 90 95
Gly His Cys Pro Trp Trp His Thr Val Ser Thr Tyr Ala Phe Gln Ser
100 105 110
Ala Phe Ala Asp Ala Asp Thr Ala Trp Gly Asn Trp Leu Ser Ser Ile
115 120 125
Thr Gly Asn Arg Lys Gly Gln Arg Val Arg Lys Pro Arg Phe Lys Ser
130 135 140
Lys His Arg Ser Arg Asp Ser Phe Arg Ile His His Asp Val His Asn
145 150 155 160
Pro Arg Ile Arg Pro Asp Ala Gly Tyr Arg Arg Ile Ile Val Pro Arg
165 170 175
Leu Gly Ser Leu Arg Leu His Gly Ser Thr Lys Arg Leu Cys Arg Ala
180 185 190
Leu Asp Arg Gly Ala Val Ile Gln Ser Val Thr Ile Ser Arg Gly Gly
195 200 205
His Arg Trp Tyr Ala Ser Ile Leu Thr Lys Ala Pro Thr Ala Thr Ala
210 215 220
Thr Pro Ser Gly Arg Gln Arg Ala Ala Gly Thr Val Gly Val Asp Ala
225 230 235 240
Gly Val His His Leu Ala Ala Leu Ser Thr Gly Asp Leu Ile Asp Asn
245 250 255
Pro Arg His Leu Asn His Ala His Thr Arg Leu Thr Lys Ala Gln Arg
260 265 270
Ala Leu Ser Arg Thr Lys Lys Gly Ser Thr Arg Arg Arg Arg Ala Ala
275 280 285
Ser Arg Val Gly Arg Ile His His His Ile Ala Glu Gln Arg Ala Thr
290 295 300
Thr Leu His Thr Leu Thr Lys Arg Leu Ala Thr Gly Trp Ala Thr Val
305 310 315 320
Ala Val Glu Asp Leu Ser Val Ala Gly Met Thr Arg Ser Ala Arg Gly
325 330 335
Thr Val Glu Gln Pro Gly Thr Asn Val Arg Ala Lys Ser Gly Leu Asn
340 345 350
Arg Ser Ile Leu Asp Thr Ser Pro Gly Glu Leu Arg Arg Gln Leu Thr
355 360 365
Tyr Lys Thr Arg Trp Tyr Gly Ser Thr Leu Ala Ile Cys Asp Arg Trp
370 375 380
Tyr Pro Ser Ser Gln Thr Cys Ser Thr Cys Gly Ala Arg Ser Lys Leu
385 390 395 400
Arg Leu Ser Gln Arg Val Phe His Cys Ala Ala Cys Gly Leu Arg Leu
405 410 415
Asp Arg Asp Val Asn Ala Ala Ile Asn Ile Ala Thr His Ala Val Val
420 425 430
Ala Val Ala Ser Asp Thr Gly Glu Thr Gln Asn Ala Arg Arg Asp Pro
435 440 445
Thr Pro Thr Pro Ala Pro Ala Glu Gln Gln Arg Gln Val Thr Asp Ala
450 455 460
Gly Arg Pro His His Thr Gly Ala Ala Thr Ser Ala Glu Gln Ser Ala
465 470 475 480
Asp His Leu Lys Thr Ala Arg Gln Pro Pro Leu Ala Leu Val Ser
485 490 495
<210> 101
<211> 494
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 101
Met Thr Ala Pro Glu Pro Gln Leu Arg Ala Tyr Arg Phe Ala Leu Asp
1 5 10 15
Leu Thr Ala Glu Gln Glu Thr Arg Cys Arg Gln His Ala Gly Ala Ala
20 25 30
Arg Trp Ala Tyr Asn His Ala Leu Ala Ala Lys Phe Ala Ala Leu Asp
35 40 45
Ala Arg Arg Ala Val Val Ala Asp Arg Thr Ala Ala Gly Asp Asp Pro
50 55 60
Lys Thr Ala Gly Ala His Ala Pro Lys Ile Pro Thr Lys Pro Thr Ile
65 70 75 80
Gln Lys Asn Leu Asn Gln Val Lys Gly Asp Ser Arg Ala Gly Ile Asp
85 90 95
Gly Leu Cys Pro Trp Phe His Glu Val Ser Thr Tyr Ala Phe Gln Ser
100 105 110
Ala Phe Ala Asp Ala Asp Thr Ala Trp Lys Asn Trp Thr Asp Ser Ile
115 120 125
Thr Gly Arg Arg Gly Gly Arg Pro Val Lys Arg Pro Arg Tyr Lys Thr
130 135 140
Lys His Arg Ser Arg Asp Ser Phe Arg Ile His His Asp Val Thr His
145 150 155 160
Pro Thr Ile Arg Leu Asp Thr Gly Tyr Arg Arg Ile Ile Val Pro Arg
165 170 175
Phe Gly Ser Leu Arg Val Tyr Asn Ser Thr Lys Arg Leu Arg Arg Ala
180 185 190
Val Ala Ala Gly Ala Val Ile Gln Ser Val Thr Ile Ser Arg Gly Gly
195 200 205
His Arg Trp Tyr Ala Ser Ile Leu Thr Lys His Pro Ser Gln Pro Ala
210 215 220
Ala Thr Ala Ser Arg Arg Gln Arg Ala Ala Gly Thr Val Gly Val Asp
225 230 235 240
Val Gly Val His His Leu Ala Ala Leu Ser Thr Gly Glu Leu Ile Asp
245 250 255
Asn Pro Arg His Leu Ala Ala Ala Arg Thr Arg Leu Thr Lys Thr Gln
260 265 270
Arg Ala Leu Ala Arg Thr Glu Lys Gly Ser Gln Arg Arg Arg Arg Ala
275 280 285
Ala Ala Arg Val Gly Arg Arg His His Glu Val Ala Glu Arg Arg Ala
290 295 300
Thr Thr Leu His His Leu Ser Lys Thr Leu Ala Thr Gln Trp Ala Val
305 310 315 320
Val Ala Ile Glu Asp Leu Asn Val Ala Gly Met Thr Arg Ser Ala Arg
325 330 335
Gly Thr Ile Glu Asn Pro Gly Thr Asn Val Ala Ala Lys Ser Gly Leu
340 345 350
Asn Arg Ala Val Ile Asp Ala Ala Pro Gly Glu Leu Arg Arg Gln Leu
355 360 365
Thr Tyr Lys Thr Arg Trp Tyr Gly Ser Thr Leu Ala Met Cys Arg Arg
370 375 380
Trp Ile Pro Thr Ser Gln Leu Cys Ser Ala Cys Gly Ala Arg Ala Lys
385 390 395 400
Leu Thr Leu Ala Glu Arg Thr Tyr His Cys Ala Ala Cys Gly Leu Ile
405 410 415
Leu Asp Arg Asp Leu Asn Ala Ala Arg Asn Ile Ala Ala His Ala Val
420 425 430
Ala Val Ala Pro Gly Thr Gly Glu Thr Leu Asn Ala Arg Arg Ala Ser
435 440 445
Thr Asp Thr Pro Pro Pro Val Ala Val Ser Val Asp Val Met Thr Arg
450 455 460
Glu Asp His Thr Thr Gly Val Ala Thr Ser Val Gly Gln Pro Ala Asp
465 470 475 480
His Pro Gln Ser Thr Cys Pro Pro Glu Tyr Pro Leu Val Ser
485 490
<210> 102
<211> 548
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 102
Met Thr Asp Thr Ser Pro Arg Pro Pro Val Val His Arg Gly Tyr Lys
1 5 10 15
Leu Arg Leu Asp Pro Asn Leu Ala Gln Leu Glu Val Leu Arg Gln Gln
20 25 30
Val Gly Thr Ala Arg Val Ala Tyr Asn Met Met Cys Ala Tyr Thr His
35 40 45
Asp Ile Gln Ala Ala Arg Asp Gly Arg Trp Lys Glu Leu Val Ser Thr
50 55 60
Gly Val Asn Phe Ser Val Ala Trp Asp Gln Val Lys Lys Glu Ala Lys
65 70 75 80
Ala Asn Phe Ala Leu Gln Tyr Pro Gly Tyr Gln Gln Phe Ser Lys Ile
85 90 95
Leu Thr Gln Gln Val Ala Arg His Arg His Ala Ala Ala Ala Ile Glu
100 105 110
Ala Gly Ala Asp Pro Ala Ala Val Trp Gly Glu Glu Glu Arg Tyr Ala
115 120 125
Thr Pro Trp Leu His Thr Ala His Arg Arg Val Leu Val Ser Gly Leu
130 135 140
Gln Met Cys Gly Asn Ser Leu Ser Asn Trp Leu Thr Ser Gln Ser Gly
145 150 155 160
Lys Arg Lys Gly Pro Ala Met Gly Pro Pro Gln Phe Lys Arg Lys His
165 170 175
His Ser Arg Glu Ser Phe Thr Ile Pro Ala Pro Glu Ala Met Gly Pro
180 185 190
Lys Gly Phe Ala Thr Tyr Lys Arg Gly Glu Ala Arg Ser Gly Glu Ile
195 200 205
Thr Asp Tyr Arg His Val Arg Leu Ser His Met Gly Thr Phe Arg Thr
210 215 220
Val Asp Ser Thr Lys Arg Leu Val Arg Ala Leu Arg Gln Gly Gly Lys
225 230 235 240
Ile Lys Ser Tyr Thr Val Ser Arg Ala Ala Asp Arg Trp Tyr Val Ser
245 250 255
Phe Leu Val Glu Arg Pro Ala Pro Gln Asn Pro Pro His Pro Thr Arg
260 265 270
Arg Gln Gln Ala Ala Gly Ala Val Gly Leu Asp Phe Gly Val Ser Arg
275 280 285
Pro Val Thr Leu Ser Thr Gly Glu Ser Ile Pro Asn Pro Arg His Leu
290 295 300
Ala Arg Ala Glu Lys Lys Ile Lys Arg Thr Gln Arg Lys Leu Ala Arg
305 310 315 320
Thr Gln Lys Gly Ser Asn Arg Arg Arg Lys Leu Ala Gln Arg Leu Ala
325 330 335
Arg His His His Thr Val Ala Leu Arg Arg His Gly Phe Leu His Glu
340 345 350
Val Thr Thr Ala Leu Ala Gly Gly Phe Ala Ala Ile Gly Val Glu Asp
355 360 365
Leu Asn Val Ala Gly Met Thr Ser Ser Ala Arg Gly Thr Val Asp Asn
370 375 380
Pro Gly Lys Asn Val Lys Gln Lys Ala Gly Leu Asn Arg Ala Ile Leu
385 390 395 400
Asp Val Ser Pro Gly Glu Phe Arg Arg Gln Leu Asp Tyr Lys Cys Thr
405 410 415
Glu Arg Gly Val Ala Leu Ile Ala Val Asp Gln Phe Phe Pro Ser Ser
420 425 430
Gln Ile Cys Ser Ala Cys Gly Ser Lys Thr Lys Met Pro Leu Ala Lys
435 440 445
Arg Val Tyr Asp Cys Gln Gln Cys Gly Ile Leu Ile Asp Arg Asp Val
450 455 460
Asn Ala Ala Ile Asn Val Leu Arg Glu Ala Ala Arg Leu Ala Gln Phe
465 470 475 480
Ser Leu Gly Asp Ser Ser Ala Pro Glu Gly Ala Glu Gly Lys Trp Pro
485 490 495
Trp Thr Pro Thr Ala His Arg Thr Arg Pro Gly Val Gly Asp Gly Ala
500 505 510
Gly Val Glu Ala Ala Arg Pro Ala Trp Ser Gln Gln Ser Ser Asn Arg
515 520 525
Leu Leu Val Pro Tyr Asn Val Arg Ile Gln Ser Glu Gln Lys His Pro
530 535 540
Leu Gln Val Arg
545
<210> 103
<211> 462
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 103
Met Thr Glu Ile Lys Arg Ala Phe Arg Phe Ala Leu Asp Pro Thr Pro
1 5 10 15
Ala Gln Thr Glu Phe Leu Gln Arg Tyr Ala Asn Ala Ser Arg Cys Ala
20 25 30
Phe Asn Phe Ala His Ala Phe Met Leu Ala His His His Asn Trp His
35 40 45
Arg Gly Arg Asn Thr Leu Ile Ala Ala Gly Met Asp Lys Ala Glu Ala
50 55 60
Thr Lys Lys Ala Pro Arg Val Lys Met Pro Gly Arg Asp Arg Ala Gln
65 70 75 80
Ala Phe Trp Arg Asp Thr Arg Gly Gln Gly Phe Val Gly Pro Leu Arg
85 90 95
Glu Gly Ala Glu Arg Ala Gly Ala Tyr Val Trp Trp Glu Gly Val Asn
100 105 110
Asn Arg Ala Tyr Tyr Thr Ala Phe Glu Asp Ala Ala Thr Ala Trp Lys
115 120 125
Asn Tyr Leu Asp Ser Ala Ala Gly Arg Arg Ala Gly Glu Arg Val Gly
130 135 140
Leu Pro Arg Phe Lys Ala Lys Gly Arg Cys Arg Glu Ser Phe Arg Leu
145 150 155 160
Val His Asn Thr Lys Lys Pro Glu Ile Arg Phe Ile Glu Pro Arg Arg
165 170 175
Leu Arg Ile Pro Gly Gly Gly Gly Gln Pro Ala Phe Thr Val Arg Leu
180 185 190
His Gln Arg Ala Ser Arg Leu Ile Ala Leu Ile Glu Ala Gly Lys Ala
195 200 205
Val Ile Thr Ser Val Thr Val Ala Arg Ala Gly His Arg Trp Tyr Ala
210 215 220
Ser Val Leu Cys Thr Val Asp Gln Ala Ile Pro Asp Gln Pro Thr Cys
225 230 235 240
Arg Gln Arg Gln Ala Gly Arg Ile Gly Val Asp Leu Gly Val Lys Ser
245 250 255
Leu Leu Ala Leu Thr Asp Pro Leu His Leu Thr Pro Gly Thr Pro Ala
260 265 270
Ala Thr Leu Val Gly Asn Pro Arg His Tyr Asp Asn Ile Ala Arg Lys
275 280 285
Leu Ala Arg Ala Gln Arg Gln Met Ala Arg Arg His Val Lys Gly Ala
290 295 300
Arg Gln Gln Ser Lys Gly Phe Gln Glu Ala Arg Asp Arg Val Ala Arg
305 310 315 320
Leu Gln Ala Gln Leu Ala Ala Arg Arg Val Thr Gly Leu His Leu Ile
325 330 335
Ser Lys Arg Leu Val Gln Gln Tyr Ser Glu Ile Ala Leu Glu Thr Leu
340 345 350
Asn Thr Lys Gly Met Thr Arg Ser Ala Lys Gly Thr Val Asp Arg Pro
355 360 365
Gly Arg Asn Val Arg Ala Lys Ala Gly Leu Asn Arg Ala Ile Leu Asp
370 375 380
Ala Ser Phe Arg Glu Leu Asn Arg Gln Ile Glu Tyr Lys Ala Arg Trp
385 390 395 400
His Gly Val Gln Val Ala Arg Val Pro Thr Phe Phe Pro Ser Ser Arg
405 410 415
Thr Cys Ser Thr Cys Gly Trp Thr Asn Thr Thr Gln Thr Leu Lys Asp
420 425 430
Arg Thr Phe His Cys Glu Ser Cys Thr Met Ile Leu Asp Arg Asp Thr
435 440 445
Asn Ala Ala Arg Asn Ile Lys Lys His Ala Val Pro Val Gln
450 455 460
<210> 104
<211> 492
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 104
Met Thr Gln Val Glu Thr Leu Arg Ala Tyr Lys Phe Ala Leu Asp Pro
1 5 10 15
Thr Ala Ala Gln Leu Ala Asp Leu Ala Arg His Ala Gly Ala Ala Arg
20 25 30
Trp Ala Phe Asn His Ala Leu Gly Ser Lys Val Ala Ala His Arg Gln
35 40 45
Trp Arg Ala Ala Val Asp Ala Leu Val Ala Gly Gly Met Pro Glu Pro
50 55 60
Gln Ala Arg Lys Ala Val Lys Val Pro Val Pro Gly Asn Gln Ala Ile
65 70 75 80
Lys Lys Thr Leu Asn Gly Leu Lys Gly Asp Ser Arg Ser Asp Pro Ala
85 90 95
Leu Pro Asp Gly Phe His Gly Pro Pro Arg Pro Cys Pro Trp Trp His
100 105 110
Glu Val Ser Thr Tyr Ala Phe Gln Ser Ala Phe Ile Asp Ala Asp Arg
115 120 125
Ala Trp Gly Asn Trp Leu Asp Ser Leu Thr Gly Arg Arg Ala Gly Gly
130 135 140
Pro Val Gly Tyr Pro Arg Phe Lys Arg Lys Gly Arg Ala Arg Asp Ser
145 150 155 160
Phe Arg Leu His His Asp Val Lys Lys Pro Thr Ile Arg Leu Asp Gly
165 170 175
Tyr Arg Arg Leu Gln Leu Pro Arg Leu Gly Ser Ile Arg Val His Asp
180 185 190
Ser Gly Lys Arg Leu Ala Arg Leu Ile Ala Lys Gly His Ala Val Ile
195 200 205
Gln Ser Val Thr Val Phe Arg Gly Gly Asn Arg Trp Tyr Ala Ser Val
210 215 220
Leu Ala Lys Val Gln Gln Asp Ile Pro Asp Lys Pro Thr Arg Ala Gln
225 230 235 240
Thr Gly Arg Gly Thr Val Gly Val Asp Trp Gly Ile Lys His Leu Ala
245 250 255
Thr Leu Ser Gln Pro Leu Asp Pro Ala Asp Pro Ala Thr Leu His Val
260 265 270
Ala Asn Pro Arg His Leu Asp Ala Trp Asn Arg Gln Leu Ala Thr Ala
275 280 285
Gln Arg Ala Leu Ser Arg Thr Glu Arg Gly Ser Lys Arg Arg Ala Lys
290 295 300
Ala Ala Arg Arg Val Gly Ala Ile Gln His Arg Ile Ala Gln Arg Arg
305 310 315 320
Ala Thr Thr Val His Leu Leu Thr Lys Arg Leu Ala Thr Gly Phe Ala
325 330 335
Thr Val Ala Val Glu Asp Leu Asn Val Arg Gly Met Ser Ala Ser Ala
340 345 350
Arg Gly Thr Val Glu Gln Pro Gly Ser Arg Val Arg Gln Lys Ala Gly
355 360 365
Leu Asn Arg Gly Ile Leu Asp Ala Ala Pro Gly Glu Leu Arg Arg Gln
370 375 380
Leu Thr Tyr Lys Thr Ser Trp Tyr Gly Ser Thr Leu Ala Val Leu Asp
385 390 395 400
Arg Trp His Pro Ser Ser Lys Thr Cys Ser Ser Cys Gly Thr Ala Lys
405 410 415
Pro Lys Leu Thr Leu Ser Glu Arg Val Phe Thr Cys Thr Thr Cys Gly
420 425 430
Leu Thr Ile Asp Arg Asp His Asn Ala Ala Val Asn Ile Ala Arg His
435 440 445
Ala Val Val Pro Leu Val Glu Gly Asp Ala Asn Ala Arg Arg Ser Pro
450 455 460
Arg Pro Ala Asp Ala Gly Arg Asp Gly His Ala Glu Arg Gln Lys Arg
465 470 475 480
Glu Gly Pro Pro Pro Gly Gly Ser Pro Arg Arg Glu
485 490
<210> 105
<211> 495
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 105
Met Thr Ser Glu Pro Thr His Glu Leu Arg Ala Tyr Arg Phe Ala Leu
1 5 10 15
Asp Leu Thr Lys Pro Gln Glu Glu Leu Cys Arg Lys His Ala Gly Ala
20 25 30
Ala Arg Trp Ala Phe Asn His Ala Leu Ala Ala Lys Phe Ala Ala Leu
35 40 45
Asp Ala Arg Arg Thr Ala Ile Ala Asp Leu Val Ala Gly Gly Met Asp
50 55 60
Pro Lys Thr Ala Ala Ala Gln Ala Pro Lys Val Pro Gly Lys Pro Ala
65 70 75 80
Ile Gln Lys Ala Leu Asn Glu Val Lys Gly Asp Asp Arg Arg Gly Leu
85 90 95
Asp Gly Leu Cys Pro Trp Trp His Glu Leu Ser Thr Tyr Ala Phe Gln
100 105 110
Ser Ala Phe Ala Asp Val Asp Thr Ala Trp Ser Asn Trp Leu Gly Ser
115 120 125
Val Thr Gly Lys Arg Ala Gly Lys Arg Leu Gly Ala Pro Lys Phe Lys
130 135 140
Ser Lys His Lys Ser Arg Asp Ser Phe Arg Ile His His Asn Val Ala
145 150 155 160
Lys Pro Ser Ile Arg Pro Asp Thr Gly Tyr Arg Arg Ile Ile Val Pro
165 170 175
Arg Phe Gly Ser Leu Arg Val His Asp Ser Thr Lys Arg Leu Ser Arg
180 185 190
Ala Leu Asp Arg Gly Ala Val Ile Gln Ser Val Thr Ile Ser Arg Gly
195 200 205
Gly His Arg Trp Tyr Ala Ser Ile Leu Thr Lys Gln Pro Ala Val Pro
210 215 220
Val Val Ala Ser Gln Arg Gln Leu Arg Ala Gly Thr Val Gly Val Asp
225 230 235 240
Ile Gly Val His His Leu Ala Ala Leu Ser Thr Gly Glu Ile Ile Asp
245 250 255
Asn Pro Gly His Leu Arg Ser Gly Gln Lys Arg Leu Thr Lys Ala Gln
260 265 270
Arg Ala Leu Ser Arg Thr Glu Lys Gly Ser Gln Arg Arg Arg Arg Ala
275 280 285
Ala Ala Lys Val Gly Arg Arg His His Glu Ile Ala Glu Arg Arg Ala
290 295 300
Ser Thr Leu His Gln Leu Thr Lys Arg Leu Ala Thr Glu Phe Ala Val
305 310 315 320
Val Ala Val Glu Asp Leu Asn Val Ala Gly Met Thr Arg Ser Ala Ala
325 330 335
Gly Thr Ile Asp Asn Pro Gly Lys Asn Val Val Gln Lys Ser Gly Leu
340 345 350
Asn Arg Ala Leu Leu Asp Val Ala Pro Gly Glu Leu Arg Arg Gln Val
355 360 365
Ala Tyr Lys Thr Arg Trp Tyr Gly Ser Thr Leu Ala Val Cys Gly Arg
370 375 380
Trp Val Pro Thr Ser Gln Leu Cys Ser Ala Cys Gly Val Arg Ala Lys
385 390 395 400
Leu Thr Leu Ala Asp Arg Ile Phe Arg Cys Ala Ala Cys Gly Leu Thr
405 410 415
Leu Asp Arg Asp Thr Asn Ala Ala Val Asn Ile Ala Ala Leu Ala Val
420 425 430
Ala Val Ala Pro Gly Thr Glu Glu Thr Arg Asn Ala Arg Arg Ala Ala
435 440 445
Ala Asp Pro Pro Pro Leu Val Gly Thr Glu Ser Val Ala Ala Leu Lys
450 455 460
Arg Glu Gly His Ala Ser Gly Val Val Thr Pro Ala Glu Gln Ser Ala
465 470 475 480
Gly His Pro Lys His Ala Asp Pro Ala Glu Tyr Pro Leu Val Ser
485 490 495
<210> 106
<211> 524
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 106
Met Thr Ser Gln Glu Val Leu Arg Ala Tyr Arg Phe Ala Leu Asp Pro
1 5 10 15
Ser Asp Ser Gln Ala Ala Leu Leu Thr Arg Tyr Ala Gly Ala Ala Arg
20 25 30
Trp Ala Phe Asn His Ala Leu Ala Ala Lys Gln Ala Glu His Gln Arg
35 40 45
Trp Arg Ala Glu Val Gln Ala Leu Val Glu Ser Gly Glu Thr Glu Ala
50 55 60
Glu Ala Arg Lys Arg Ile Lys Leu Val Val Pro Ser Lys Pro Ala Ile
65 70 75 80
Gln Lys Ala Trp Val Thr Gly Arg Gly Asp Ser Arg Ser Ala Val Asp
85 90 95
Gly Ser Cys Pro Trp Trp Trp Glu Val Asn Asn His Cys Phe Gln Ser
100 105 110
Gly Phe Ile Asp Ala Asp Ala Ala Trp Arg Asn Trp Leu Ser Ser Leu
115 120 125
Arg Gly Gln Arg Gly Gly His Arg Val Gly Tyr Pro Arg Tyr Lys Lys
130 135 140
Lys Gly Arg Ser Arg Asp Ser Phe Arg Leu His His Asp Ala Lys Arg
145 150 155 160
Pro Ser Ile Arg Leu Val Gly Tyr Arg Arg Ile Asn Leu Pro Lys Phe
165 170 175
Gly Glu Val Arg Ile His Asn Ser Gly Lys Pro Leu Ala Arg Met Val
180 185 190
Gly Arg Gly Gln Ala Val Ile Gln Ser Val Thr Val Ser Arg Ala Gly
195 200 205
Ser Arg Trp Tyr Ala Ser Val Leu Cys Arg Val Gln Gln Glu Ile Pro
210 215 220
Gln Arg Leu Thr Ala Arg Gln Arg Ala Gly Gly Val Val Gly Val Asp
225 230 235 240
Leu Gly Ser Thr His Leu Ala Val Leu Ser Gly Arg Leu Arg Gln Ala
245 250 255
Asp His Asp Ser Asn Val Val Glu His Pro Arg His Leu Gln Arg Cys
260 265 270
Met Thr Arg Leu Thr Lys Ala Gln Arg Ala Leu Ser Lys Thr Val Lys
275 280 285
Gly Ser Val Arg Arg Gly Arg Ala Ala Arg Lys Val Ala Arg Ile His
290 295 300
His Val Val Ala Glu Arg Arg Ala Thr His Leu His Ala Leu Thr Arg
305 310 315 320
Gln Leu Ala Glu Asn Phe Ser His Ile Ala Val Glu Gly Phe Asp Val
325 330 335
Gln Gly Leu Met Lys Arg Pro Pro Val Pro Gly Glu Gly Arg Arg Ala
340 345 350
Ser Ser Ile Thr Ala Arg Phe Asn Arg Tyr Leu Leu Asp Ala Ala Pro
355 360 365
Gly Glu Val Arg Arg Gln Leu Ala Tyr Lys Ala Leu Trp Tyr Gly Ser
370 375 380
Thr Met Val Val Leu Thr Pro Gly Glu Pro Val Asn Gln Val Cys Ser
385 390 395 400
Arg Cys Gly Ser Gln Asn Ser Arg Val Ser Val Ser Asp Ser Arg Phe
405 410 415
Ser Cys Arg Asp Cys Gly Phe Ser Ile Gly Arg Arg His Asn Ala Ala
420 425 430
Gln Asn Ile Arg His Ala Ala Gln Arg Met Ile Gly Asn Val Thr Ser
435 440 445
Gly Ser Gly Glu Thr Glu Lys Ala Arg Gly Val Ala Val Arg Pro Ser
450 455 460
Ala Gly Ser Ala Lys Gly Ala Gln Arg Arg Ser Glu Lys Thr Ser Gly
465 470 475 480
His Arg Gly Leu Met Pro Pro Arg Glu Ser Asp Leu Ala Ala Pro Cys
485 490 495
Val Pro His Gln Gly Asp Met Gln Gly Asp Gln Ser Thr Gly Ser Thr
500 505 510
Pro Glu Arg Asp Gly Phe Cys Asp Pro Gly Cys Gly
515 520
<210> 107
<211> 513
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 107
Met Thr Thr Ala Arg Ala Asn Glu Ile Pro Thr Cys Gln Thr Gln His
1 5 10 15
Leu Arg Ser Thr His Met Thr Asp Val Glu Leu Ser Ala Tyr Arg Phe
20 25 30
Ala Leu Asp Thr Thr Pro Ala Gln Leu Thr Met Leu Arg Gln His Ala
35 40 45
Gly Ala Ala Arg Trp Ala Tyr Asn His Ala Leu Gly Val Lys Phe Ala
50 55 60
Ala Leu Asp Glu Arg Lys Thr Val Ile Ala Gly Leu Val Glu Gln Gly
65 70 75 80
Leu Asp Pro Lys Thr Ser Ala Ala Gln Ala Pro Lys Ile Pro Thr Lys
85 90 95
Pro Ala Ile Gln Lys Ala Leu Asn Thr Thr Lys Gly Asp Asp Arg Ile
100 105 110
Ser Ala Ala Gly Asp Cys Pro Trp Trp His Thr Val Ser Thr Tyr Ala
115 120 125
Phe Gln Ser Ala Phe Ala Asp Ala Asp Thr Ala Trp Lys Asn Trp Leu
130 135 140
Ala Ser Leu Thr Gly Lys Arg Ser Gly Pro Ile Gly Ala Pro Arg Phe
145 150 155 160
Lys Ser Lys His Arg Ser Arg Asp Ser Phe Arg Ile His His Asp Val
165 170 175
Asn Asn Pro Thr Ile Arg Pro Asp Asp Gly Tyr Arg Arg Ile Ile Val
180 185 190
Pro Arg Leu Gly Ser Leu Arg Val His Asp Ser Thr Lys Arg Leu Lys
195 200 205
Arg Ala Ile Asp Arg Gly Ala Val Ile Gln Ser Val Thr Ile Ser Arg
210 215 220
Gly Gly His Arg Trp Tyr Ala Ser Ile Leu Val Lys Ala Pro Ala Ala
225 230 235 240
His Ala Ala Pro Thr Arg Arg Gln Arg Gln Ala Gly Thr Val Gly Val
245 250 255
Asp Leu Gly Val His His Leu Ala Ala Leu Ser Thr Gly Asp Ile Ile
260 265 270
Asp Asn Pro Arg His Leu Ala Ala Gly Gln Lys Arg Leu Thr Lys Ala
275 280 285
Gln Arg Ala Leu Ser Arg Thr Glu Lys Gly Ser Asn Arg Arg Arg Arg
290 295 300
Ala Ala Ala Arg Val Gly Arg Arg His His Glu Ile Thr Glu Arg Arg
305 310 315 320
Ala Thr Thr Leu His Thr Leu Thr Lys His Leu Ala Thr Asn Trp Ala
325 330 335
Thr Val Ala Ile Glu Asp Leu Asn Val Ala Gly Met Thr Arg Ser Ala
340 345 350
Arg Gly Thr Ile Asp Asn Pro Gly Thr Asn Val Arg Ala Lys Ala Gly
355 360 365
Leu Ser Arg Ala Ile Leu Asp Thr Ser Pro Gly Glu Leu Arg Arg Gln
370 375 380
Leu Thr Tyr Lys Thr Gly Trp Tyr Gly Ser Thr Leu Ala Ile Cys Asp
385 390 395 400
Arg Trp Phe Pro Ser Ser Gln Gln Cys Cys Glu Cys Lys Val Arg Thr
405 410 415
Lys Leu Arg Leu Ser Gln Arg Val Phe Thr Cys Pro Ala Cys Gly Tyr
420 425 430
Gly Pro Ile Asp Arg Asp Val His Ala Ala Arg Asn Ile Ala Ala Tyr
435 440 445
Ala Ala Val Ala Ser Asp Thr Gly Glu Thr Leu Thr Ala Arg Arg Asp
450 455 460
Thr Ala Glu Ala Pro Thr Arg Val Gly Arg Arg Arg Gly Ala Val Asp
465 470 475 480
Ala Gly Arg Pro His Arg Glu Thr Gly Ala Ala Thr Pro Ala Glu Gln
485 490 495
Pro Ala Gly His Pro Lys His Ala Asp Gln Arg Thr Leu Pro Leu Val
500 505 510
Ser
<210> 108
<211> 632
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 108
Met Thr Thr Glu Thr Val Met Gln Gln Leu Ala Phe Lys Val Ala Leu
1 5 10 15
Asp Pro Thr Pro Arg Gln Ala Arg Ala Met Gly Ser His Ala Gly Gly
20 25 30
Ala Arg Phe Ala Tyr Asn Trp Gly Ile Ala Arg Ile Ala Glu Ala Leu
35 40 45
Lys Gln Arg Glu Ala Glu Arg Gln Ala Gly Gly Glu Pro Thr Thr Pro
50 55 60
Val Pro Asp His Phe Ala Leu Cys Lys Leu Trp Thr Gln Trp Lys Asp
65 70 75 80
Thr Ala Glu Trp Val Asp Arg Ala Thr Gly Glu Val Thr Arg Gly Val
85 90 95
Pro Trp Val Ala Glu Asn Phe Val Gly Thr Tyr Gln Ala Ala Leu Arg
100 105 110
Asp Ala Ala Arg Ala Trp Ala Asn Tyr Phe Asp Ser Arg Thr Gly Gln
115 120 125
Arg Ala Gly Arg Ala Ile Gly Lys Pro Arg Phe Lys Ser Lys Arg Thr
130 135 140
Ala Arg Lys Ser Phe Gln Val His Gly Ser Ser Leu Arg Leu Val Ser
145 150 155 160
Ala Val Lys Val Asn Leu Pro Lys Ile Gly Thr Val Thr Val Met Ser
165 170 175
Asp Asp Ser Arg His Pro Ala Met Ala Arg Ser Arg Arg Arg Val Pro
180 185 190
Gly Gln Arg His Met Gly Asn Arg Arg Arg Ser Arg Gln Leu Trp Gln
195 200 205
Arg Leu Arg His Gly Gln Ala Leu Ala Glu Arg Ala Ala Glu Ile Leu
210 215 220
Ser Ala Ala Tyr Ala Asp Gly Asn Pro Asp Arg Val Leu Glu His Leu
225 230 235 240
Thr Ala Leu Ala Asp Glu Arg Ala Arg Ala Arg Ala Val Ala Ala Ala
245 250 255
Glu Glu Arg Leu Gln Lys Ala Lys Ala Ala Ala Ala Lys Ala Lys Pro
260 265 270
Glu Lys Arg Glu Lys Ala Gln Gln Arg Val Ala Gln Ala Glu Glu Ala
275 280 285
Leu Arg Arg Ala Gln Ala Ile Thr Ala Asn Arg Lys Asp Gly Trp Ser
290 295 300
Ala Arg Lys Leu Ala Glu Val Cys Arg Thr Gly Leu Ile Thr Pro Val
305 310 315 320
Gln Ala Ala Asp Leu Ala Glu Val Phe Gly Leu Gly Glu Gln Gln Arg
325 330 335
Ala Ala Leu Ala Glu Ala Ala Leu Gln Pro Arg Leu Val Lys Ala Thr
340 345 350
Val Thr Leu Gly Ala Asp Gly Leu Trp Trp Leu Ser Leu Ser Ala Glu
355 360 365
Val Pro Val Arg Val Arg Thr Ser Pro Ser Arg Arg Gln Arg Glu Arg
370 375 380
Gly Ala Thr Gly Leu Asp Leu Gly Val Arg Asn Val Val Thr Ser Ser
385 390 395 400
Lys Arg Leu Gly Gly Asp Gln Asn Leu Pro Asn Pro Arg Tyr Leu Glu
405 410 415
Gln Ala Leu Val Glu Leu Arg Ala Ala Gln Lys His Leu Ser Arg Cys
420 425 430
Thr Pro Gly Ser Ala Arg His Ala Lys Ala Lys Ala Arg Val Gly Leu
435 440 445
Ile His Ala Asp Val Ala Arg Leu Arg Glu Gln His His His Arg Leu
450 455 460
Ser Thr Arg Leu Val Arg Thr Phe Ala Gly Ile Ala Val Glu Gly Tyr
465 470 475 480
Asp Leu Gln Glu Leu Ala Arg Arg Gly Ser Glu Gly Leu Pro Gly Glu
485 490 495
Val Arg Arg Arg Arg Asn Arg Ala Leu Ala Asp Thr Gly Ala Gly Met
500 505 510
Leu Arg Gln Gln Leu Gln Tyr Lys Thr Ala Arg Tyr Gly Ser His Leu
515 520 525
Val Val Ala Glu Pro Asp Ala Pro Thr Gly Arg Thr Cys Ala Thr Cys
530 535 540
Gly Gln Ala Lys Ala Lys Pro Val Pro Pro Tyr His Glu Val Phe Ala
545 550 555 560
Cys Glu Ala Cys Gly Arg Thr Arg Pro Arg Arg Leu Asn Thr Ala Leu
565 570 575
Ala Leu Glu Ala Val Ala Ala Ser Ser Gly Ser Pro His Gly Gly Ser
580 585 590
Asp Gln Pro Arg Gly Glu Asp Val Arg Pro Ala Ala Ser Arg Arg Gly
595 600 605
Gly Gln Ser Ser Met Lys Arg Ala Ala Arg Ser Arg Pro Pro Gly Arg
610 615 620
Gly Gln Thr Gly Thr Pro Gly Pro
625 630
<210> 109
<211> 495
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 109
Met Thr Thr Gly Asp Val Glu Met Arg Ala Tyr Arg Phe Ala Leu Asp
1 5 10 15
Leu Thr Pro Thr Gln Glu Gln Gln Cys Arg Gln His Ala Gly Ala Ala
20 25 30
Arg Trp Ala Tyr Asn His Ala Leu Ala Ala Lys Phe Ala Ala Leu Asp
35 40 45
Ala Arg Gln Ala Thr Ile Thr Thr Leu Val Glu Gln Gly Val Asp Pro
50 55 60
Lys Val Ala Ala Thr Gln Ala Pro Lys Ile Pro Thr Lys Pro Ala Ile
65 70 75 80
Gln Lys Ala Leu Asn Ala Thr Lys Gly Asp Asn Arg Thr Asp Leu Asp
85 90 95
Gly Asp Cys Pro Trp Trp His Thr Val Ser Thr Tyr Ala Phe Gln Ser
100 105 110
Ala Met Ile Asp Val Asp Thr Ala Trp Lys Asn Trp Leu Asp Ser Ile
115 120 125
Thr Gly Lys Arg Arg Gly Gln Arg Val Ser Arg Pro Arg Phe Lys Thr
130 135 140
Lys His Arg Ser Arg Asp Ser Phe Arg Ile His His Asp Val Lys Asn
145 150 155 160
Pro Ser Ile Arg Pro Asp Thr Gly Tyr Arg Arg Ile Ile Val Pro Arg
165 170 175
Leu Gly Ser Leu Arg Val His Asp Ser Thr Lys Lys Leu Cys Arg Ala
180 185 190
Leu Asp Arg Gly Ala Ile Ile Gln Ser Val Thr Ile Ser Arg Gly Gly
195 200 205
His Arg Trp Tyr Ala Ser Ile Leu Thr Lys Thr Pro Ala Ala Ile Thr
210 215 220
Ala Ala Pro Thr Arg Arg Gln Arg Lys Ala Gly Thr Val Gly Val Asp
225 230 235 240
Val Gly Val His His Leu Ala Ala Leu Ser Thr Gly Asp Ile Ile Asp
245 250 255
Asn Pro Arg His Leu Asp Arg Ala Arg Ala Arg Leu Asn Lys Ala Gln
260 265 270
Arg Lys Leu Ser Arg Thr Gln Arg Gly Ser Thr Arg Arg Arg Arg Ala
275 280 285
Ala Ala Val Val Gly Arg Arg His His Glu Val Ala Glu Gln Arg Ala
290 295 300
Thr Thr Leu His Thr Leu Thr Lys Arg Leu Ala Thr Gly Trp Ala Thr
305 310 315 320
Val Ala Ile Glu Asp Leu Asn Val Leu Gly Met Thr Arg Ser Ala Arg
325 330 335
Gly Thr Ile Asp Gln Pro Gly Thr Asn Val Arg Ala Lys Ala Gly Leu
340 345 350
Asn Arg Ala Ile Leu Asp Ala Ala Pro Gly Glu Leu Arg His Gln Leu
355 360 365
Asn Tyr Lys Ala Arg Trp Tyr Ala Ser Thr Ile Ala Val Cys Asp Arg
370 375 380
Trp Tyr Pro Ser Ser Gln Thr Cys Ser Thr Cys Gly Ala Arg Thr Lys
385 390 395 400
Leu Arg Leu Ser Gln Arg Ala Tyr Arg Cys Ala Glu Cys Gly Leu Asp
405 410 415
Leu Asp Arg Asp Val Asn Ala Ala Ile Asn Ile Ala Thr Tyr Ala Val
420 425 430
Ala Val Thr Pro Asp Thr Glu Glu Thr Gln Asn Ala Arg Arg Gly Arg
435 440 445
Ala Pro Ala Pro Thr Ser Val Gly Arg Arg Arg Gln Val Thr Asp Ala
450 455 460
Gly Arg Pro His His His Gly Ala Ala Thr Ser Ala Glu Gln Ser Thr
465 470 475 480
Asp His Pro Lys Thr Ala Asp Gln Arg Pro Leu Thr Leu Val Ser
485 490 495
<210> 110
<211> 499
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 110
Met Thr Thr Val Glu His Asp Leu Arg Ala Tyr Arg Leu Ala Leu Asp
1 5 10 15
Leu Thr Ala Glu Gln Thr Ser Ala Val Arg Gln His Ala Gly Ala Ala
20 25 30
Arg Trp Ala Phe Asn His Ala Leu Ala Ala Lys Leu Ala Ala Leu Asp
35 40 45
Ala Arg Met Ser Thr Ile Ala Ala Ala Thr Ala Ala Gly Ala Asp Pro
50 55 60
Ala Ala Ala Arg Ala Ser Ala Pro Arg Ile Pro Thr Lys Pro Thr Ile
65 70 75 80
Gln Lys Ala Leu Asn Gln Ile Lys Gly Asp Asp Arg Thr Gly Val Asp
85 90 95
Gly Leu Cys Pro Trp Trp Asn Ser Val Ser Thr Tyr Thr Phe Gln Ser
100 105 110
Ala Phe Ala Asp Ala Asp Gln Ala Trp Ser Asn Trp Met Asn Ser Leu
115 120 125
Thr Gly Lys Arg Ala Gly Arg Arg Val Gly Arg Pro Arg Phe Lys Ser
130 135 140
Lys Arg Arg Ser Arg Asp Ser Phe Arg Ile His His Asp Val Lys Asn
145 150 155 160
Pro Thr Ile Arg Pro Asp Thr Gly Tyr Arg Arg Ile Ile Val Pro Arg
165 170 175
Leu Gly Ser Val Arg Val His Asn Ser Thr Lys Arg Leu Arg Arg Ala
180 185 190
Leu Asp Arg Gly Ala Val Ile Gln Ser Val Thr Ile Ser Arg Ala Gly
195 200 205
His Arg Trp Tyr Ala Ala Val Leu Val Lys Ala Pro Arg Thr Ala Ala
210 215 220
Ala Pro Thr Thr Arg Gln Arg Ala Ala Gly Arg Ile Gly Val Asp Leu
225 230 235 240
Gly Val His His Leu Ala Ala Leu Ser Asp Gly Thr Ile Ile Asp Asn
245 250 255
Pro Arg His His Arg His Ala Arg Ala Arg Leu Thr Lys Ala Gln Arg
260 265 270
Ala Leu Ala Arg Thr Gln Lys Gly Ser Asn Arg Arg Arg Arg Ala Ala
275 280 285
Ala Thr Val Gly Arg Arg His His Glu Leu Ala Glu Arg Arg Ala Thr
290 295 300
Thr Thr His Thr Leu Thr Lys Gln Leu Thr Thr Arg Trp Ala Thr Val
305 310 315 320
Ala Ile Glu Asp Leu Asn Val Ala Gly Met Thr Arg Ser Ala Arg Gly
325 330 335
Thr Ile Glu Ala Pro Gly Thr Asn Val Ala Ala Lys Ala Gly Leu Asn
340 345 350
Arg Ala Ile Leu Asp Ala Ser Phe Gly Glu Leu Arg Arg Gln Leu Asn
355 360 365
Tyr Lys Thr Asp Trp Tyr Gly Ser Thr Leu Ala Val Cys Asp Arg Trp
370 375 380
Ala Pro Thr Ser Gln Leu Cys Ala Glu Cys Gly Glu Arg Ala Asn Leu
385 390 395 400
Arg Leu Ser Gln Arg Thr Phe Arg Cys Pro Ala Cys Thr Tyr Gly His
405 410 415
Glu Thr Ile Val Asp Arg Asp Val Asn Ala Ala Arg Asn Ile Ala Ala
420 425 430
Leu Ala Val Ala Val Ala Pro Gly Thr Glu Glu Thr Gln Thr Ala Arg
435 440 445
Arg Ala Glu Pro Asp Pro Arg Pro Arg Gly Gly Asn Arg Ser Gly Pro
450 455 460
Ala Val Lys Arg Glu Asp Arg Thr Leu Pro Gly Ala Ala Thr Pro Val
465 470 475 480
Glu Gln Ser Thr Gly His Pro Lys Pro Ala Asp Gln Arg Ser Leu Pro
485 490 495
Leu Val Ser
<210> 111
<211> 449
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 111
Met Val Ala Val Gln Arg Ala Phe Lys Phe Ala Leu Asp Pro Thr Pro
1 5 10 15
Thr Gln Leu Glu Arg Leu Ser Arg Tyr Ala Gly Ala Ser Arg Met Ala
20 25 30
Tyr Asn Phe Ala Leu Gly Leu Lys Gly Gln Thr His Gln Gln Trp Arg
35 40 45
Ser Leu Val Asp Glu Leu Ile Ala Ala Gly Leu Glu Glu Lys Glu Ala
50 55 60
His Arg Arg Val Gln Ile Lys Thr Pro Thr Lys Pro Thr Val Gln Lys
65 70 75 80
Ile Phe Asn Gln Leu Arg Gly Gly Pro Gly Glu Gly Pro Gly Gly Leu
85 90 95
Ala Pro Trp His His Glu Val Ala Thr Trp Cys Phe Gln Ser Ala Trp
100 105 110
Met Asp Ala Asp Gln Ala Trp Lys Asn Trp Leu Asp Ser Leu Thr Gly
115 120 125
Lys Arg Ala Gly His Lys Val Gly Tyr Pro Arg Phe Lys Lys Lys Asn
130 135 140
Arg Ser Arg Asp Ser Phe Arg Leu His His Asp Val Arg Glu Pro Thr
145 150 155 160
Leu Arg Leu Glu Ser Tyr Arg Arg Leu Asn Leu Gly Lys Phe Gly Val
165 170 175
Val Arg Leu His Glu Ser Gly Lys Arg Met His Arg Leu Val Ser Ser
180 185 190
Gly Ala Ala Val Val Lys Ser Val Thr Val Ser Arg Ser Gly Ser Arg
195 200 205
Trp Tyr Ala Ser Val Gln Cys Glu Ile Gln Thr Glu Arg Pro Ala Pro
210 215 220
Thr Arg Arg Gln Arg Val Asn Gly Arg Val Gly Met Asp Val Gly Val
225 230 235 240
His Tyr Leu Ala Ala Leu Ser Gln Pro Ile Gln Asp Tyr Val Leu Ile
245 250 255
Asp Asn Pro Arg His Leu Ser Arg Ala Ser Lys Arg Leu Ala Arg Ala
260 265 270
Gln Arg Ala Leu Ser Arg Thr Gln Lys Gly Ser Met Arg Arg Arg Lys
275 280 285
Ala Ala Ala Arg Val Gly Lys Leu His Gln Glu Val Ala Gln Gln Arg
290 295 300
Ala Thr Thr Leu His Trp Leu Ser Lys Arg Leu Ala Thr Thr Phe Ala
305 310 315 320
Cys Val Ala Ile Glu Asp Leu Asn Val Ala Gly Met Thr Arg Ser Ala
325 330 335
Arg Gly Thr Val Glu Glu Pro Gly Ser Asn Val Ala Ala Lys Ala Gly
340 345 350
Leu Asn Ser Ser Ile Leu Asp Ala Ala Pro Ala Glu Leu Arg Arg Gln
355 360 365
Leu Glu Tyr Lys Thr Ser Trp Tyr Gly Ser Gln Leu Ala Val Leu Asp
370 375 380
Arg Trp Phe Pro Ser Ser Lys Thr Cys Ser Ala Cys Gly Trp Gln Asn
385 390 395 400
Pro Asn Gln Thr Leu Ala Asp Arg Val Phe Ser Cys Ala Glu Cys Gly
405 410 415
Leu Arg Leu Asp Arg Asp Leu Asn Ala Ala Arg Asn Ile Ala Ala His
420 425 430
Ala Glu Val Val Ser Ala Ser Gly Thr Gly Thr Pro Gly Arg Gly Gly
435 440 445
Pro
<210> 112
<211> 726
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 112
Met Val Arg Ser Arg Leu Arg Trp Met Val Thr Arg His Arg Arg Arg
1 5 10 15
Thr Ala Leu Val His Gly Cys Cys Leu Leu Pro Trp Arg Trp Arg Ser
20 25 30
Thr Leu Arg Cys Ser Ser Gly Arg Ser Glu Gly Ala Glu Gly Glu Ala
35 40 45
Gly Thr Thr Pro Glu Ser Pro Ile Cys Asp Leu Asp Tyr Glu Lys Asn
50 55 60
Arg Gly Phe Leu His Tyr Phe Ser Arg His Ser His Ile Gln Gly Gly
65 70 75 80
Asn Leu Arg Arg Pro Asn Thr Pro Glu Gln Ser Ser Phe Leu Asp Ala
85 90 95
Thr Pro Gln Gln Lys Ser His Val Ser Val Leu Trp Leu Trp Ala Asn
100 105 110
Leu Phe Ser Lys Tyr Ser Trp Leu Pro Gln Asn Thr Pro Leu Ser Cys
115 120 125
Gln Gly Tyr Pro Leu Trp Ser Leu Gln Glu Ile Ser Lys Gln Val Ile
130 135 140
Pro Ile Ser Arg Arg Arg Gly Gly Lys Ala Met Ala Glu Lys Thr Ser
145 150 155 160
Thr Asp Asp Gly Val Leu Tyr Arg Ala Tyr Lys Phe Arg Leu Asp Pro
165 170 175
Asn Gln Ala Gln Lys Thr Glu Leu Met Arg Cys Val Gly Ala Ala Arg
180 185 190
His Thr Tyr Asn Leu Leu Thr Thr Tyr Asn Leu Gln Ile Leu His Asn
195 200 205
Lys Gln Glu Tyr Arg Arg Thr Arg Ala Ala Glu Gly Ala Asp His Glu
210 215 220
Thr Ile Asn Gly Glu Leu Lys Lys Leu Arg Glu Lys Asp Pro Ala Tyr
225 230 235 240
Gln Ile Leu Tyr Lys Ala Gly Tyr Lys Thr Gly Asn Glu Tyr Val Pro
245 250 255
Gly Tyr Arg Gln Leu Tyr Leu Thr Pro Glu Ile Lys Arg His Arg Ala
260 265 270
Ala Ser Lys Ala Ile Ala Ala Gly Ala Asp Pro Ala Val Val Trp Pro
275 280 285
Glu Thr Glu Arg Tyr Ser Glu Pro Trp Leu His Ser Ile Asn Ser Arg
290 295 300
Val Leu Asp Ser Gly Leu Leu Asn Ala Gly Lys Ala Trp Lys Asn Tyr
305 310 315 320
Lys Glu Ser Leu Met His Leu Arg Ala Gly Ala Arg Met Gly Val Pro
325 330 335
Arg Arg Lys Arg Lys Gly Val Ser Arg Asp Ser Phe Thr Val Asp Arg
340 345 350
Asp Pro Asn Asn Asp Glu Val Gly Ala Tyr Gly Thr Pro Tyr Lys Lys
355 360 365
Gly Thr Pro Glu Phe Ala Arg Arg Thr Ala Gln Leu Lys Arg Arg Gly
370 375 380
Ile Lys Thr Ala Pro Thr Ile Glu Asp Tyr Arg His Val Arg Leu Ser
385 390 395 400
His Leu Gly Val Ile Arg Thr His Asn Thr Thr Lys Pro Leu Val Lys
405 410 415
Ala Val Arg Ala Gly Ala Asn Val Lys Ser Tyr Thr Val Ser Arg Ala
420 425 430
Ala Asn Arg Trp Tyr Val Ser Ile Leu Val Lys Leu Ser Arg Thr Pro
435 440 445
Ala Thr Pro Thr Arg Ala Gln Arg Ser Ala Gly Ala Val Gly Val Asp
450 455 460
Leu Gly Val Arg Tyr Leu Ala Ala Leu Ser Asp Glu Gln Ala Pro Gln
465 470 475 480
Arg Phe Ala Gln Tyr Pro Ser Leu Glu Phe Thr Asp Asn Gly Ala Pro
485 490 495
Thr Leu Ala Asn Pro Arg Trp Ala Arg Thr Ala Glu Lys Arg Leu Val
500 505 510
Arg Leu Gln Arg Ala Leu Ala Arg Ala Gln Lys Gly Ser Asn Arg Arg
515 520 525
Ala Arg Leu Val Arg Gln Ile Ala Arg His His His Leu Val Ala Leu
530 535 540
Arg Arg Glu Ser Gly Leu His Gln Val Ser Lys Arg Leu Ala Thr Gly
545 550 555 560
Tyr Thr Leu Ile Gly Leu Glu Asp Leu Ala Val Ala Gly Met Thr Ala
565 570 575
Ser Ala Ala Gly Thr Ile Lys Ala Pro Gly Lys Asn Val Arg Gln Lys
580 585 590
Ala Gly Leu Asn Arg Ser Ile Leu Asp Ala Ala Phe Gly Thr Leu Arg
595 600 605
Arg Gln Leu Glu Tyr Lys Ala Ser Trp Tyr Gly Ser Gln Val Gln Ile
610 615 620
Ile Asp Arg Phe Phe Ala Ser Ser Gln Thr Cys Ser Ala Cys Gly Ala
625 630 635 640
Arg Ala Lys Thr Lys Leu Gly Leu His Val Arg Val Phe Glu Cys Ala
645 650 655
Ala Cys Gly Ala Arg Ile Asp Arg Asp Val Asn Ala Ala Arg Asn Ile
660 665 670
Arg Ala Glu Ala Val Arg Met Tyr Glu Ala Gln Leu Ala Pro Gly Thr
675 680 685
Gly Glu Ser Leu Asn Gly Arg Gly Ala Thr Asp Ser Asp Ala Ala Gly
690 695 700
Ser Val Val Leu Gly Asp Val Ala Leu Asp Val Ser Arg Pro Ala Ala
705 710 715 720
Ser Gly Gly Gly Ser Pro
725
<210> 113
<211> 494
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 113
Met Val Arg Ser Val Gly Glu Asp Leu Arg Ala Tyr Arg Phe Ala Leu
1 5 10 15
Asp Leu Thr Pro Ala Gln Ala Arg Ala Val Ala Glu His Ala Gly Ala
20 25 30
Ala Arg Trp Ala Tyr Asn His Ala Leu Ala Val Lys Phe Ala Ala Leu
35 40 45
Lys Gln Arg Gln Thr Val Ile Ala Glu Leu Val Ala Ala Gly Val Asp
50 55 60
Ala Gln Val Ala Ala Arg Gln Ala Pro Arg Ile Pro Ser Lys Pro Gln
65 70 75 80
Ile Gln Lys Asp Leu Asn Thr Ala Lys Gly Asp Ser Arg Thr Gly Val
85 90 95
Asp Gly Leu Cys Pro Trp Trp Trp Thr Val Ser Thr Tyr Ala Phe Gln
100 105 110
Ser Ala Met Ala Asp Ala Asp Arg Ala Trp His Asn Trp Ile Ser Ser
115 120 125
Val Thr Gly Gln Arg Ala Gly Lys Arg Val Gly Arg Pro Arg Phe Lys
130 135 140
Ser Lys His Arg Cys Arg Asp Ser Phe Arg Ile His His Asp Val Lys
145 150 155 160
Gln Pro Thr Ile Arg Pro Asp Thr Ser Gly Tyr Arg Arg Leu Ile Val
165 170 175
Pro Arg Leu Gly Ser Leu Arg Thr His Asp Ser Thr Lys Arg Leu Arg
180 185 190
Arg Ala Leu Asp Arg Gly Ala Val Ile Gln Ser Val Thr Ile Thr Arg
195 200 205
Gly Gly His Arg Trp Tyr Ala Ser Val Leu Val Lys Thr Leu Asp Ala
210 215 220
Pro Ala Val Gly Pro Thr Arg Ala Gln Arg Lys Ala Gly Ile Val Gly
225 230 235 240
Val Asp Ile Gly Val His Thr Leu Ala Ala Leu Ser Thr Gly Glu Leu
245 250 255
Ile Ala Asn Pro Arg His Leu Asn Ser Ser Arg Asp Arg Leu Ile Ala
260 265 270
Ala Gln Arg Ala Leu Ala Arg Thr Val Lys Gly Ser Asn Arg Arg Arg
275 280 285
Arg Ala Ala Arg Val Leu Ser Arg Arg His His Glu Leu Ala Glu Arg
290 295 300
Arg Ala Thr Thr Leu His Gln Leu Thr Lys Arg Leu Ala Thr Gly Trp
305 310 315 320
Ser Val Val Ala Val Glu Asp Leu Gln Val Ala Gly Met Thr Arg Ser
325 330 335
Ala Arg Gly Thr Val Asp Asn Pro Gly Arg Asn Val Arg Ala Lys Ala
340 345 350
Gly Leu Asn Arg Ala Ile Leu Asp Val Ala Pro Ser Glu Leu Arg Arg
355 360 365
Gln Leu Thr Tyr Lys Thr Gly Trp Tyr Gly Ser Ser Leu Val Val Cys
370 375 380
Asp Arg Phe Tyr Pro Ser Thr Gln Thr Cys Ser Ala Cys Gly Ala Lys
385 390 395 400
Ala Lys Leu Thr Leu Ala Asp Arg Val Phe Arg Cys Ala Ala Cys Gly
405 410 415
Phe Gly Ile Asp Arg Asp Leu Asn Ala Ala Arg Asn Ile Ala Ala His
420 425 430
Ala Ala Val Ala Ser Gly Thr Arg Glu Thr Ile Asn Ala Arg Arg Val
435 440 445
Asp Gln Arg His Pro Pro Gln Val Gly Ala Arg Arg Arg Pro Ala Met
450 455 460
Lys Arg Glu Gly His His Ala Cys Gln Thr Val Val Val Thr Ser Ala
465 470 475 480
Gly Gln Pro Ala Asp His Pro Lys Thr Ser Val Arg Ser Thr
485 490
<210> 114
<211> 522
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 114
Pro Pro Met Thr Ala Thr Asp Ala Ala Thr Pro Pro Ala Gly Val Leu
1 5 10 15
Arg Ala Val Arg Val Ile Leu Asp Pro Thr Pro Arg Gln Ala Ala Ala
20 25 30
Leu Ala Ser His Ala Gly Ala Ala Arg Ala Ala Phe Asn Tyr His Leu
35 40 45
Ala Glu Lys Met Ala Ala His Arg Ala Tyr Ala Ala Glu Val Ala Tyr
50 55 60
Arg Thr Tyr Ile Asp Phe Ala Gly Leu Asp Pro Asn Ala Ala Tyr Ala
65 70 75 80
Ala Ala Lys Lys Ala Val Thr Ala Ala Lys Thr His Arg Ile Pro Gly
85 90 95
Met Pro Asp Ser Ile Lys Lys Met Tyr Ala Asp Pro Asp Tyr Gly Trp
100 105 110
Leu Pro Asp Ile Asn Arg Tyr Ala Ile Ser Ser Gly Met Arg Ala Ala
115 120 125
Asp Thr Ala Trp Ser Asn Trp Leu Ala Ser Leu Ser Gly Glu Arg Ser
130 135 140
Gly Pro Arg Val Gly Tyr Pro Arg Phe Lys Lys Lys Gly Arg Ala Arg
145 150 155 160
Ser Ala Phe Thr Leu Tyr His Asp Val Lys Ala Pro Ser Leu Arg Pro
165 170 175
Asp Gly Pro Arg Arg Trp Arg Phe Pro Ala Lys Val Gly Gly Ser Ile
180 185 190
Arg Leu Lys Gly Asn Val Arg Arg Leu Ala Arg Arg Ile Ala Arg Gly
195 200 205
Thr Ala Cys Val Lys Asn Val Thr Val Ala Gln Ser Gly Gly Gln Trp
210 215 220
Val Ala Ser Ile Leu Val Glu Asp Phe Ala Gln Leu Pro Ala Gly Pro
225 230 235 240
Thr Arg Arg Gln Arg Ala Ala Gly Thr Val Gly Val Asp Val Gly Val
245 250 255
Thr His Leu Ala Ala Leu Ser Thr Gly Glu His Val Pro Asn Pro Arg
260 265 270
His Leu Arg Arg Ala Gln Ala Arg Leu Val Lys Ala Gln Arg Ala Leu
275 280 285
Ser Arg Thr Gln Trp Arg Val Glu Gly Thr Gly Glu Leu Val Ala Val
290 295 300
Pro Val Arg Gly Arg Arg His Val Glu Thr Ser Gly Arg Arg Lys Ala
305 310 315 320
Arg Ala Arg Leu Ala Lys Ala His Ala Glu Val Ala Gln Ala Arg Ala
325 330 335
Thr Thr Leu His Ala Leu Thr Lys Thr Leu Ala Thr Ser Tyr Ala Val
340 345 350
Val Val Val Glu Asp Leu Ala Val Lys Gly Met Thr Ala Ala Pro Ala
355 360 365
Ala Arg Pro Asp Pro Ala Ser Pro Gly Ala Phe Leu Pro Asn Gly Ala
370 375 380
Ala Ala Lys Gly Gly Leu Asn Arg Ala Val Leu Asp Ala Ala Phe Gly
385 390 395 400
Glu Ile Arg Arg Gln Leu Thr Tyr Lys Thr Ser Trp Tyr Arg Ser Thr
405 410 415
Leu Gln Val Ala Pro Arg Phe Tyr Pro Ser Ser Lys Thr Cys Ser Ala
420 425 430
Cys Gly Ala Val Lys Ala Lys Leu Arg Leu Ala Glu Arg Val Phe Ser
435 440 445
Cys Pro Ala Cys Gly His Ala Glu Asp Arg Asp Ile Asn Ala Ala Lys
450 455 460
Asn Leu Ala Ala Arg Ser Trp Thr Pro Ala Glu Ser Gly Ser Leu Lys
465 470 475 480
Thr Pro Ala Glu Pro Ala His Ala Ser Arg Arg Arg Ala Asp Asn Asp
485 490 495
Ala Gly Arg Pro Trp Pro Pro Gln Ala Pro Val Thr Val Gly Glu Glu
500 505 510
Ser Pro Thr Arg Pro Pro Pro Ser Pro Ala
515 520
<210> 115
<211> 653
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 115
Gln Ala Ala His Arg Asp Ala Asp Val Ala Trp Gln Arg Phe Leu His
1 5 10 15
Gly Lys Ser Arg Arg Pro Arg Phe Lys Lys Lys Gly Lys Cys Thr Glu
20 25 30
Ser Phe Gln Val His Gly Asp Asp Leu Gly Ile Pro Glu Thr Thr Glu
35 40 45
Ala Glu Arg Ile Arg Phe Pro Glu Ala Val Ala Ala Leu Arg Pro His
50 55 60
Gly Arg Arg Pro Val Gln Gln Gln Thr Ala Gln His Ile Thr Leu Pro
65 70 75 80
Lys Ile Gly Pro Ala Arg Val Met Ser Asp Asp Ser Leu His Pro Ala
85 90 95
Met Arg Arg Ser Arg Arg Arg Gly Ser Gly Val Thr Pro Ala His Arg
100 105 110
Pro Gly Arg Lys Leu Lys Ala Ala Arg Ala Arg Leu Glu Ala Ala Gly
115 120 125
Arg Ala Tyr Glu His Ala Ala Glu Thr Met Pro Glu Val Pro Ala Ser
130 135 140
Thr Leu His Glu Val Ser Ala Ala Arg Asp Ala Ala Ala Lys Ala Glu
145 150 155 160
Gln Ala Val Ala Tyr Ala Arg Gln Arg Asp Ala Gly His Gly Arg His
165 170 175
Met Gly Asn Arg Arg Arg Phe Arg Gln Leu His Arg His Met Arg Lys
180 185 190
Ser Gly Glu Asn Ala Ala Arg Leu Gly Pro Leu Leu Arg Gln Val Arg
195 200 205
Glu Asp Ala Gly Trp Thr Leu Asp Gln Ala Val Thr Leu Leu Gly Thr
210 215 220
Glu Ala Asp Ala Arg Ala Val Ala Ala Val Arg Ala Arg Leu Ala Ala
225 230 235 240
Ala Glu Asp Ala Ala Asp Ile Ala Ala Ala Ala Ala Ala Glu Ala Val
245 250 255
Ala Ala Gly Asp Ala Glu Gly Ala Ala Ala Ala Glu Lys Asp Gln Arg
260 265 270
Asn Ala Arg Asn Arg Val Lys Gly Ala Leu Lys Arg Leu Gly Asp Leu
275 280 285
Ala Ala Ala Lys Pro Gly Glu Lys Ala Ser Arg Thr Asp Arg Trp Ser
290 295 300
Ala Ala Lys Leu Lys Lys Met Glu Ala Thr Gly Val Thr Leu Gly Leu
305 310 315 320
Asp Gln Ala Ser Val Ile Cys Ser Ala Tyr Arg Leu Gly Gly Gln Leu
325 330 335
Arg Glu Gln Val Thr Asp Leu Ala Ala Gln Ala Arg Ile Val Arg Ala
340 345 350
Thr Ile Thr Leu Gly Ala Asp Gly Leu Trp Trp Cys Ser Val Gly Ala
355 360 365
Glu Ile Pro Tyr Ala Val Arg Thr Ala Pro Ser Lys Arg Gln Glu Ala
370 375 380
His Gly Thr Ala Gly Ile Asp Phe Gly Thr Ala Arg Ile Met Thr Val
385 390 395 400
Thr Gly Arg Arg Pro Val Gly Ala Pro Ser Tyr Tyr Glu Lys Glu Leu
405 410 415
Ala Ala Leu Gly Ala Ala Gln Arg His Ala Gly Arg Thr Val Pro Gly
420 425 430
Ser Gly Arg His Arg Lys Ala Lys Ala Arg Ala Gly Leu Ile His Ala
435 440 445
Asp Val Ala Arg Leu Arg Ala Asp Thr Leu His Arg Ala Thr Thr Ser
450 455 460
Leu Ser Arg Asn Phe Ser Val Leu Ser Phe Glu Gly Phe Asn Val Gln
465 470 475 480
Gln Leu Met Arg Asp Gly Ser Gln Gly Met Pro Arg Gln Ala Arg Gly
485 490 495
Arg Arg Asn Arg Ser Leu Ala Asp Ala Gly Ile Gly Met Ala Arg Glu
500 505 510
Met Leu Arg Gln Lys Gly Pro Arg Cys Gly Ala Thr Val Val Thr Leu
515 520 525
Gly Pro Asp Val Ile Thr Ile Thr Gly Pro Arg Leu Glu Asp Ala Arg
530 535 540
Glu Thr Asp Leu Pro Pro Asp Ala Lys Pro Asn Gly Thr Cys Ser Gly
545 550 555 560
Cys Gly Gln Ala Arg Thr Lys Pro Ala Arg Pro His Glu Ala Leu Tyr
565 570 575
Arg Cys Asp Ala Cys Gly Arg Val Arg Leu Arg Thr Leu Asn Ser Ala
580 585 590
Glu Asn Ile Arg Arg Trp Ala Asp Glu Ala Leu Thr Ala Asp Pro Gly
595 600 605
Cys Ala Ile Ser Gly Pro Asp Gln Ser Arg Gly Gly Asp Glu Pro Ala
610 615 620
Thr Ala Arg Ala Gly Arg Ser Pro Val Lys Arg Ala Ala Thr Thr Ser
625 630 635 640
Ser Arg Pro Arg Arg Gly Glu Ala Gly Thr Pro Gly Gly
645 650
<210> 116
<211> 427
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 116
Arg His Ala His Ala Ala Arg Ile Ala Phe Asn Tyr Ala Leu Ala Ala
1 5 10 15
Lys Ile Ala Ala His Glu Glu Trp Lys Arg Gln Val Ala Ala Leu Val
20 25 30
Glu Ser Gly Val Pro Glu Lys Asp Ala Arg Glu Gln Thr Asn Ala Asn
35 40 45
Val Arg Ile Pro Ala Lys Pro Asp Asn Gln Lys Ala Phe Asn Gln Val
50 55 60
Arg Gly Asp Ser Arg Lys Gly Ile Asp Gly Ile Cys Pro Trp Phe His
65 70 75 80
Glu Val Ser Thr Tyr Ala Phe Gln Ser Ala Phe Glu Asp Ala Asp Ala
85 90 95
Ala Trp Arg Asn Trp Leu Ala Ser Gln Lys Gly Glu Arg Lys Gly Gln
100 105 110
Lys Val Gly Tyr Pro Lys Leu Lys Arg Arg Tyr Arg Arg Arg Glu Ser
115 120 125
Phe Arg Leu Tyr Gly Thr Ile Arg Leu Ala Ser Tyr Arg Arg Leu Tyr
130 135 140
Ile Pro Lys Ile Lys Glu Ile Arg Ile His Gly Thr Gly Lys His Leu
145 150 155 160
Ala Arg Leu Val Glu Arg Gly Asp Ala Val Ile Gln Ser Val Thr Ile
165 170 175
Ser Arg Glu Gly His Arg Trp Tyr Ala Ser Ile Leu Ala Lys Val Thr
180 185 190
Met Asp Ile Pro Asp Gln Pro Thr Arg Ser Gln Arg Glu Arg Gly Ala
195 200 205
Val Gly Val Asp Leu Gly Val Lys Thr Leu Ala Ala Leu Ser Lys Pro
210 215 220
Leu Asp Pro Ala Asn Pro Ala Ser Gln Leu Ile Pro Asn Ser Lys Pro
225 230 235 240
Leu Glu Ala Ala Gln Arg Arg Leu Leu Lys Ala Gln Arg Ala Leu Ser
245 250 255
Arg Lys Gln Lys Gly Ser Arg Arg Trp Val Lys Thr Lys Arg Arg Ile
260 265 270
Ala Lys Leu Asn His Gln Ile Ala Val Arg Arg Ala Ala Ala Ile His
275 280 285
Gln Val Thr Lys Gln Leu Ala Thr Arg Phe Ala Thr Val Ala Ile Glu
290 295 300
Asp Leu Asn Ile Arg Gly Met Thr Ser Ser Ala Arg Gly Thr Ile Asp
305 310 315 320
Asn Pro Gly Arg Asn Val Arg Ala Lys Ala Gly Leu Asn Arg Ala Ile
325 330 335
Leu Asp Ala Ala Pro Gly Glu Phe Arg Arg Gln Leu Glu Tyr Lys Thr
340 345 350
Ala Trp Tyr Gly Ser Arg Leu Val Val Val Asp Arg Trp Leu Pro Ser
355 360 365
Ser Lys Thr Cys Ser Ala Cys Gly Thr Val Lys Glu His Leu Arg Leu
370 375 380
Ser Asp Arg Val Phe Glu Cys Glu Thr Cys Gly Ala Arg Leu Asp Arg
385 390 395 400
Asp Leu Asn Ala Ala Arg Asn Ile Glu Ala Ala Ala Thr Arg Val Cys
405 410 415
Ser Leu Pro Ser Pro Leu Met Glu Gly Arg Arg
420 425
<210> 117
<211> 474
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 117
Tyr Asn Trp Gly Leu Gln Arg Ile Ala Glu Ala Leu Asp Ala Arg Gln
1 5 10 15
Ala Glu Lys Thr Ala Gly Leu Lys Pro Thr Thr Arg Val Pro Gly His
20 25 30
Phe Asp Leu Cys Lys Ala Trp Thr Gln Trp Lys Asp Thr Thr Glu Trp
35 40 45
Thr Asp Arg Asp Thr Gly Glu Val Thr Val Gly Val Pro Trp Val Ala
50 55 60
Arg Asn Phe Val Gly Thr Tyr Gln Ala Ala Leu Arg Asp Ala Ala Ala
65 70 75 80
Ala Trp Lys Arg Phe Phe Asp Ser Arg Ala Gly Arg Leu Ala Gly Arg
85 90 95
Arg Val Gly Arg Pro Arg Phe Lys Lys Lys Gly Arg Ser Arg Ala Ser
100 105 110
Phe Gln Val His Gly Ala Gln Leu Gln Val Val Asp Ala His His Val
115 120 125
Lys Leu Pro Lys Val Gly Val Val Lys Thr His Glu Ser Thr Arg Lys
130 135 140
Leu Leu Arg Arg Ile Arg Lys Gly Asp Thr Thr Cys Pro Thr Cys Lys
145 150 155 160
Ala Thr Gly Gln Val Pro Gly Lys Asp Thr Pro Lys Lys Cys Pro Ala
165 170 175
Cys Lys Gly Ser Gly Arg Val Pro Val Ala Arg Leu Val Arg Gly Thr
180 185 190
Val Ser Gln Gly Ser Asp Gly Arg Trp Ser Ile Ala Leu Thr Val Glu
195 200 205
Val Val Arg Glu Ile Arg Thr Gly Pro Ser Asp Arg Gln Arg Ala Gly
210 215 220
Gly Thr Ile Gly Val Asp Leu Gly Val Arg Ser Leu Val Val Thr Ser
225 230 235 240
Ala Gly Glu Gln Phe Glu His Pro Arg His Leu Glu Ala Ala Leu Asp
245 250 255
Arg Leu Ala Ala Ala Gln Arg Ala Leu Ala Arg Cys Glu Lys Gly Ser
260 265 270
Lys Arg Arg Ala Lys Ala Ala Ala Arg Val Ala Arg Leu His Ala Arg
275 280 285
Val Ala Asn Leu Arg Arg Asp Ala Thr His Lys Leu Thr Ser Arg Leu
290 295 300
Val His Ser His Glu Arg Ile Gly Val Glu Gly Trp Asp Val Gln Gln
305 310 315 320
Thr Ala Gln Arg Gly Ser Ala Asp Val Pro Lys Arg Val Arg Arg Asp
325 330 335
Arg Asn Arg Val Leu Ala Asp Ala Asn Leu Gly Glu Leu Arg Trp Gln
340 345 350
Leu Gln Ser Lys Ala Ala Trp Tyr Gly Cys Gln Val Gln Val Ala Asp
355 360 365
Arg His Ala Ala Thr Gly Arg Thr Cys Ser Ala Cys Gly Gln Val Arg
370 375 380
Ala Lys Pro Val Pro Pro Phe Glu Glu Gln Phe Thr Cys Pro Ala Cys
385 390 395 400
Gly Trp Cys Gly Asp Arg Arg Trp Asn Thr Ala Arg Val Leu Ala Ala
405 410 415
Val Ala Arg Gly Glu His Gln Gln Tyr Asp Asp Ala Ser Ser Gly Gly
420 425 430
Glu Ser Leu Asn Ala Arg Gly Gly Asp Val Arg Pro Ala Ala Leu Arg
435 440 445
Arg Gly Gly Arg Ser Ser Leu Lys Arg Glu Ala Arg Ala Arg Pro Pro
450 455 460
Gly Arg Gly Glu Thr Gly Ile Pro Gly Pro
465 470
<210> 118
<211> 29
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 118
gucggcccca cgcguguagg gagcgaucg 29
<210> 119
<211> 250
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 119
gaacgcggcc cggaacaucg aacgccacgc agugcugauc gaucgaaacg ucgccugcga 60
uaggcgggag acgcuaaacg cccguggagc auccauaaga ccaaccaccu cucggggcgg 120
uaggcacgac gcaucgaagc gggaaggcuc cggcgcucgg ccugagucac cucagcagag 180
ugaucugcug acgcucccaa ccuugaauaa cgaaacggca acgccuccau agcggugcag 240
gucaauaagg 250
<210> 120
<211> 279
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 120
gaacgcggcc cggaacaucg aacgccacgc agugcugauc gaucgaaacg ucgccugcga 60
uaggcgggag acgcuaaacg cccguggagc auccauaaga ccaaccaccu cucggggcgg 120
uaggcacgac gcaucgaagc gggaaggcuc cggcgcucgg ccugagucac cucagcagag 180
ugaucugcug acgcucccaa ccuugaauaa cgaaacggca acgccuccau agcggugcag 240
gucaauaagg gucggcccca cgcguguagg gagcgaucg 279
<210> 121
<211> 1518
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 121
atggtgcaga cggagatcct gaaggcgttt cggtttgcgt tggatccgac gtctgtgcag 60
gttgctgccc tttcccggca tgctggtgcg gccaggtggg cgttcaacca tgctctcgcg 120
gccaaggtcg gcgcgcatga gcggtggcgg gcggaggtag ccaagcttgt cggggacggt 180
gttcctgagg agcaggctcg taggcaggtg cgcgtcccgg ttccgatgaa gccggcgatc 240
cagaaggcgt tgaacgcggt caagggtgac tctcgcaagg gcttggatgg tgcgtgcccg 300
tggtggcatg aggtcaacac ttacgcgttc cagtccgctt tcatcgacgc ggaccaggcg 360
tggaagaact ggttggactc gttgtcgggg aagcgggtcg gccgccgggt ggggtacccg 420
cggttcaaga agaaggggcg ggcccgcgac tcgtttcgct tgcaccacga cgtgaagaag 480
ccaagcatcc ggctggccgg gtaccggcgg ttgcggctgc cgaggatcgg ggaagtccgg 540
cttcatgact cggggaagcg tctggcacgg ctgatcgacc gaggtgacgc ggtggtgcag 600
tcggtgacgg tgtcgcgggg tggccatcgc tggtatgcgt ctgtgctgtg caaggtcacc 660
gtgcaggtac cggatcggcc gtcacggcgc cagcgggaac gaggcgctgt tggggtggac 720
cttggcgtta aggtcctcgc ggctttgtcc aagccactgg tggtcgacga cccgtcgagt 780
gccctggtgc ggaatccgca gcatctgcgg caggccgagc ggcgtctact gaaggctcag 840
cgggcgttgg cgcgtacaca aaaaggttca gcccgacgcg agaaggccaa gcggcgagtt 900
ggtcgcgcgc atcacgaggt tgctgtgcgc cggcatgctg cgctccatca gatcaccaag 960
cgcctgacca ccggcttcgc ggtcgtcgcg cttgaggatc tgaacgtggc cggtatgaca 1020
aggtcagcgc ggggaacagt ggcagcgccg ggaaagaacg tgcggcagaa ggcaggcctg 1080
aaccgcgtca tcctggactc cgctccagcc gagttgcgtc gccaggtgaa ctacaaggcc 1140
acctggtatg gatctgagct ggcggtcgca gaccgctggt tcccctcgag taagacctgt 1200
tcgggttgcg gatggcaaaa cccacacctg aagctgtccg acagggtgtt tcgttgcaca 1260
gactgcgggc tggtgatgga ccgggacatg aacgcggccc ggaacatcga acgccacgca 1320
gtgctgatcg atcgaaacgt cgcctgcgat aggcgggaga cgctaaacgc ccgtggagca 1380
tccataagac caaccacctc tcggggcggt aggcacgacg catcgaagcg ggaaggctcc 1440
ggcgctcggc ctgagtcacc tcagcagagt gatctgctga cgctcccaac cttgaataac 1500
gaaacggcaa cgcctcca 1518
<210> 122
<211> 1518
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 122
atggtgcaga cagagatcct gaaggccttt cggttcgccc tcgatcctac ttccgtccag 60
gtggccgccc tgagccggca cgccggggcc gccaggtggg ccttcaacca cgccctcgcc 120
gccaaggtgg gggcccacga gcggtggcgg gccgaggtgg ccaagctcgt gggcgatggc 180
gtgcccgagg agcaggcccg gcggcaggtc cgggtccccg tgcctatgaa gccagccatc 240
cagaaggccc tgaacgccgt gaagggggat tcccggaagg gcctggatgg ggcctgccca 300
tggtggcacg aggtgaacac ctacgccttc cagagcgcct ttattgacgc cgaccaggca 360
tggaagaact ggctcgacag cctgagcggc aagagagtgg ggaggagggt gggctacccc 420
cggtttaaga agaagggccg ggctcgggat agctttagat tgcaccacga tgtgaagaag 480
cccagcatca ggctggctgg ctacagaagg ctgaggctgc ctaggattgg cgaggtgagg 540
ctgcacgaca gcggcaagcg gctcgccagg ctcattgaca gaggcgacgc cgtggtgcag 600
tccgtgactg tgagtagggg cggacacagg tggtacgcct ccgtgctctg caaggtgaca 660
gtgcaggtgc ccgatcggcc cagcaggcgg cagcgggaga ggggcgccgt gggagtggac 720
ctgggggtga aggtgctggc cgccctgtcc aagcccctgg tcgtcgacga cccaagcagc 780
gccctggtga gaaaccccca gcacctgagg caggctgaga ggcggctgct caaggcccag 840
cgggccctgg ccaggacaca gaagggcagc gccaggagag agaaggccaa gcggcgggtg 900
gggagggccc atcacgaggt ggccgtccgg cggcacgccg ccctgcacca gatcaccaag 960
cggctgacca cagggttcgc cgtggtggcc ctggaggatc tgaacgtcgc cggcatgacc 1020
aggtccgcca gggggacagt ggccgcccca gggaagaacg tccggcagaa ggccgggctg 1080
aaccgggtca tcctggacag cgcccctgcc gagctgcgga ggcaggtgaa ctacaaggcc 1140
acctggtacg ggagcgagct ggccgtggcc gacaggtggt tcccctccag caagacctgc 1200
agcgggtgcg gctggcagaa cccccacttg aagctctccg atagggtgtt taggtgcacc 1260
gactgcggcc tggtcatgga tagagatatg aacgccgcta ggaacatcga gaggcacgct 1320
gtgttgattg ataggaacgt ggcctgcgac cggcgggaga ctttgaacgc caggggggcc 1380
agcatccggc ccaccacaag ccgggggggg cggcacgacg ccagcaagag ggaggggagc 1440
ggggcccggc cagagtcccc ccagcagtct gatctgctca cactccccac actgaacaac 1500
gaaaccgcca cacccccc 1518
<210> 123
<211> 279
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 123
gaacgcggcc cggaacaucg aacgccacgc agugcugauc gaucgaaacg ucgccugcga 60
uaggcgggag acgcuaaacg cccguggagc auccauaaga ccaaccaccu cucggggcgg 120
uaggcacgac gcaucgaagc gggaaggcuc cggcgcucgg ccugagucac cucagcagag 180
ugaucugcug acgcucccaa ccuugaauaa cgaaacggca acgccuccau agcggugcag 240
gucaauaagg gucggcccca cgcguguagg gagcgaucg 279
<210> 124
<211> 8198
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 124
tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg 60
gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg 120
ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc 180
tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc 240
acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 300
acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag 360
ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc 420
gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa 480
ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga 540
tctcaagaag atcatcttat taatcagata aaatatttct agatttcagt gcaatttatc 600
tcttcaaatg tagcacctga agtcagcccc atacgatata agttgtgacg taaaatgaga 660
cgttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg 720
cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat 780
cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt 840
tcagtcagtt gctcaatgta cctataacca gaccgttcag ctgggtcatc caccggatca 900
attcccctgc tcgcgcaggc tgggtgccaa gctctcgggt aacatcaagg cccgatcctt 960
ggagcccttg ccctcccgca cgatgatcgt gccgtgatcg aaatccagat ccttgacccg 1020
cagttgcaaa ccctcactga tccggctcac ggtaactgat gccgtatttg cagtaccagc 1080
gtacggccca cagaatgatg tcacgctgaa aatgccggcc tttgaatggg ttcatgtgca 1140
gctccatcag caaaagggga tgataagttt atcaccaccg actatttgca acagtgccgt 1200
tgatcgtgct atgatcgact gatgtcatca gcggtggagt gcaatgtcgt gcaatacgaa 1260
tggcgaaaag ccgagctcat cggtcagctt ctcaaccttg gggttacccc cggcggtgtg 1320
ctgctggtcc acagctcctt ccgtagcgtc cggcccctcg aagatgggcc acttggactg 1380
atcgaggccc tgcgtgctgc gctgggtccg ggagggacgc tcgtcatgcc ctcgtggtca 1440
ggtctggacg acgagccgtt cgatcctgcc acgtcgcccg ttacaccgga ccttggagtt 1500
gtctctgaca cattctggcg cctgccaaat gtaaagcgca gcgcccatcc atttgccttt 1560
gcggcagcgg ggccacaggc agagcagatc atctctgatc cattgcccct gccacctcac 1620
tcgcctgcaa gcccggtcgc ccgtgtccat gaactcgatg ggcaggtact tctcctcggc 1680
gtgggacacg atgccaacac gacgctgcat cttgccgagt tgatggcaaa ggttccctat 1740
ggggtgccga gacactgcac cattcttcag gatggcaagt tggtacgcgt cgattatctc 1800
gagaatgacc actgctgtga gcgctttgcc ttggcggaca ggtggctcaa ggagaagagc 1860
cttcagaagg aaggtccagt cggtcatgcc tttgctcggt tgatccgctc ccgcgacatt 1920
gtggcgacag ccctgggtca actgggccga gatccgttga tcttcctgca tccgccagag 1980
gcgggatgcg aagaatgcga tgccgctcgc cagtcgattg gctgagctca tgagcggaga 2040
acgagatgac gttggagggg caaggtcgcg ctgattgctg gggcaacacg tgaaaggcga 2100
gatcaccaag gtagtcggca aataatgtct aacggtaccc ggggatcctc tagagtcgac 2160
ctgcataatg tgcctgtcaa atggacgaag cagggattct gcaaacccta tgctactccg 2220
tcaagccgtc aattgtctga ttcgttacca attatgacaa cttgacggct acatcattca 2280
ctttttcttc acaaccggca cggaactcgc tcgggctggc cccggtgcat tttttaaata 2340
cccgcgagaa atagagttga tcgtcaaaac caacattgcg accgacggtg gcgataggca 2400
tccgggtggt gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg cgccagctta 2460
agacgctaat ccctaactgc tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa 2520
catgctgtgc gacgctggcg atatcaaaat tgctgtctgc caggtgatcg ctgatgtact 2580
gacaagcctc gcgtacccga ttatccatcg gtggatggag cgactcgtta atcgcttcca 2640
tgcgccgcag taacaattgc tcaagcagat ttatcgccag cagctccgaa tagcgccctt 2700
ccccttgccc ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt 2760
catccgggcg aaagaacccc gtattggcaa atattgacgg ccagttaagc cattcatgcc 2820
agtaggcgcg cggacgaaag taaacccact ggtgatacca ttcgcgagcc tccggatgac 2880
gaccgtagtg atgaatctct cctggcggga acagcaaaat atcacccggt cggcaaacaa 2940
attctcgtcc ctgatttttc accaccccct gaccgcgaat ggtgagattg agaatataac 3000
ctttcattcc cagcggtcgg tcgataaaaa aatcgagata accgttggcc tcaatcggcg 3060
ttaaacccgc caccagatgg gcattaaacg agtatcccgg cagcagggga tcattttgcg 3120
cttcagccat acttttcata ctcccgccat tcagagaaga aaccaattgt ccatattgca 3180
tcagacattg ccgtcactgc gtcttttact ggctcttctc gctaaccaaa ccggtaaccc 3240
cgcttattaa aagcattctg taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca 3300
aaagtgtcta taatcacggc agaaaagtcc acattgatta tttgcacggc gtcacacttt 3360
gctatgccat agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc 3420
aactctctac tgtttctcca tacccgtttt tttgggctag cgaaaagatg tttcgtgaag 3480
ccgtcgacgc ttataaaaaa tggatattaa tactgaaact gagatcaagc aaaagcattc 3540
actaaccccc tttcctgttt tcctaatcag cccggcattt cgcgggcgat attttcacag 3600
ctatttcagg agttcagcca tgaacgctta ttacattcag gatcgtcttg aggctcagag 3660
ctgggcgcgt cactaccagc agctcgcccg tgaagagaaa gaggcagaac tggcagacga 3720
catggaaaaa ggcctgcccc agcacctgtt tgaatcgcta tgcatcgatc atttgcaacg 3780
ccacggggcc agcaaaaaat ccattacccg tgcgtttgat gacgatgttg agtttcagga 3840
gcgcatggca gaacacatcc ggtacatggt tgaaaccatt gctcaccacc aggttgatat 3900
tgattcagag gtataaaacg aatgagtact gcactcgcaa cgctggctgg gaagctggct 3960
gaacgtgtcg gcatggattc tgtcgaccca caggaactga tcaccactct tcgccagacg 4020
gcatttaaag gtgatgccag cgatgcgcag ttcatcgcat tactgatcgt tgccaaccag 4080
tacggcctta atccgtggac gaaagaaatt tacgcctttc ctgataagca gaatggcatc 4140
gttccggtgg tgggcgttga tggctggtcc cgcatcatca atgaaaacca gcagtttgat 4200
ggcatggact ttgagcagga caatgaatcc tgtacatgcc ggatttaccg caaggaccgt 4260
aatcatccga tctgcgttac cgaatggatg gatgaatgcc gccgcgaacc attcaaaact 4320
cgcgaaggca gagaaatcac ggggccgtgg cagtcgcatc ccaaacggat gttacgtcat 4380
aaagccatga ttcagtgtgc ccgtctggcc ttcggatttg ctggtatcta tgacaaggat 4440
gaagccgagc gcattgtcga aaatactgca tacactgcag aacgtcagcc ggaacgcgac 4500
atcactccgg ttaacgatga aaccatgcag gagattaaca ctctgctgat cgccctggat 4560
aaaacatggg atgacgactt attgccgctc tgttcccaga tatttcgccg cgacattcgt 4620
gcatcgtcag aactgacaca ggccgaagca gtaaaagctc ttggattcct gaaacagaaa 4680
gccgcagagc agaaggtggc agcatgacac cggacattat cctgcagcgt accgggatcg 4740
atgtgagagc tgtcgaacag ggggatgatg cgtggcacaa attacggctc ggcgtcatca 4800
ccgcttcaga agttcacaac gtgatagcaa aaccccgctc cggaaagaag tggcctgaca 4860
tgaaaatgtc ctacttccac accctgcttg ctgaggtttg caccggtgtg gctccggaag 4920
ttaacgctaa agcactggcc tggggaaaac agtacgagaa cgacgccaga accctgtttg 4980
aattcacttc cggcgtgaat gttactgaat ccccgatcat ctatcgcgac gaaagtatgc 5040
gtaccgcctg ctctcccgat ggtttatgca gtgacggcaa cggccttgaa ctgaaatgcc 5100
cgtttacctc ccgggatttc atgaagttcc ggctcggtgg tttcgaggcc ataaagtcag 5160
cttacatggc ccaggtgcag tacagcatgt gggtgacgcg aaaaaatgcc tggtactttg 5220
ccaactatga cccgcgtatg aagcgtgaag gcctgcatta tgtcgtgatt gagcgggatg 5280
aaaagtacat ggcgagtttt gacgagatcg tgccggagtt catcgaaaaa atggacgagg 5340
cactggctga aattggtttt gtatttgggg agcaatggcg atgacgcatc ctcacgataa 5400
taagcttcct gctgaacatc aaaggcaaga aaacatctgt tgtcaaagac agcatccttg 5460
aacaaggaca attaacagtt aacaaataaa aacgcaaaag aaaatgccga tattgactac 5520
cggaagcagt gtgaccgtgt gcttctcaaa tgcctgattc aggctgtcta tgtgtgactg 5580
ttgagctgta acaagttgtc tcaggtgttc aatttcatgt tctagttgct ttgttttact 5640
ggtttcacct gttctattag gtgttacatg ctgttcatct gttacattgt cgatctgttc 5700
atggtgaaca gctttaaatg caccaaaaac tcgtaaaagc tctgatgtat ctatcttttt 5760
tacaccgttt tcatctgtgc atatagctat actgatttcg tcagactcac agtcaaacat 5820
gccggtcagt tggcctggtg atggcgggat cgttgtatat ttcttgacac cttttcggca 5880
ccgccctaaa attctgcgtc ctcataatat atgaggcgat ttattacgtg tttacgaagc 5940
aaaagctaaa accaggagct atttaatggt gcagacggag atcctgaagg cgtttcggtt 6000
tgcgttggat ccgacgtctg tgcaggttgc tgccctttcc cggcatgctg gtgcggccag 6060
gtgggcgttc aaccatgctc tcgcggccaa ggtcggcgcg catgagcggt ggcgggcgga 6120
ggtagccaag cttgtcgggg acggtgttcc tgaggagcag gctcgtaggc aggtgcgcgt 6180
cccggttccg atgaagccgg cgatccagaa ggcgttgaac gcggtcaagg gtgactctcg 6240
caagggcttg gatggtgcgt gcccgtggtg gcatgaggtc aacacttacg cgttccagtc 6300
cgctttcatc gacgcggacc aggcgtggaa gaactggttg gactcgttgt cggggaagcg 6360
ggtcggccgc cgggtggggt acccgcggtt caagaagaag gggcgggccc gcgactcgtt 6420
tcgcttgcac cacgacgtga agaagccaag catccggctg gccgggtacc ggcggttgcg 6480
gctgccgagg atcggggaag tccggcttca tgactcgggg aagcgtctgg cacggctgat 6540
cgaccgaggt gacgcggtgg tgcagtcggt gacggtgtcg cggggtggcc atcgctggta 6600
tgcgtctgtg ctgtgcaagg tcaccgtgca ggtaccggat cggccgtcac ggcgccagcg 6660
ggaacgaggc gctgttgggg tggaccttgg cgttaaggtc ctcgcggctt tgtccaagcc 6720
actggtggtc gacgacccgt cgagtgccct ggtgcggaat ccgcagcatc tgcggcaggc 6780
cgagcggcgt ctactgaagg ctcagcgggc gttggcgcgt acacaaaaag gttcagcccg 6840
acgcgagaag gccaagcggc gagttggtcg cgcgcatcac gaggttgctg tgcgccggca 6900
tgctgcgctc catcagatca ccaagcgcct gaccaccggc ttcgcggtcg tcgcgcttga 6960
ggatctgaac gtggccggta tgacaaggtc agcgcgggga acagtggcag cgccgggaaa 7020
gaacgtgcgg cagaaggcag gcctgaaccg cgtcatcctg gactccgctc cagccgagtt 7080
gcgtcgccag gtgaactaca aggccacctg gtatggatct gagctggcgg tcgcagaccg 7140
ctggttcccc tcgagtaaga cctgttcggg ttgcggatgg caaaacccac acctgaagct 7200
gtccgacagg gtgtttcgtt gcacagactg cgggctggtg atggaccggg acatgaacgc 7260
ggcccggaac atcgaacgcc acgcagtgct gatcgatcga aacgtcgcct gcgataggcg 7320
ggagacgcta aacgcccgtg gagcatccat aagaccaacc acctctcggg gcggtaggca 7380
cgacgcatcg aagcgggaag gctccggcgc tcggcctgag tcacctcagc agagtgatct 7440
gctgacgctc ccaaccttga ataacgaaac ggcaacgcct ccatagcggt gcaggtcaat 7500
aagggtcggc cccacgcgtg tagggagcga tcgcgagacc attggtctca ggccggcatg 7560
gtcccagcct cctcgctggc gccggctggg caacatgctt cggcatggcg aatgggaccc 7620
aattattgaa caccctaacg ggtgtttttt tgtttctggt ctacctggct ggttggcgta 7680
ctgttgtggt aaatacccat atggacagtt ttccctttga tatgtaacgg tgaacagttg 7740
ttctactttt gtttgttagt cttgatgctt cactgataga tacaagagcg tcgggtgatg 7800
ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt 7860
tctatcagct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg caaaagcacc 7920
gccggacatc agcgctagcg gagtgtatac tggcttacta tgttggcact gatgagggtg 7980
tcagtgaagt gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat 8040
gtgatacagg atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact 8100
gcggcgagcg gaaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat 8160
acttaacagg gaagtgagag ggccgcggca aagccgtt 8198
<210> 125
<211> 1952
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 125
tttacaccgt tttcatctgt gcatatagct atactgattt cgtcagactc acagtcaaac 60
atgccggtca gttggcctgg tgatggcggg atcgttgtat atttcttgac accttttcgg 120
caccgcccta aaattctgcg tcctcataat atatgaggcg atttattacg tgtttacgaa 180
gcaaaagcta aaaccaggag ctatttaatg gtgcagacgg agatcctgaa ggcgtttcgg 240
tttgcgttgg atccgacgtc tgtgcaggtt gctgcccttt cccggcatgc tggtgcggcc 300
aggtgggcgt tcaaccatgc tctcgcggcc aaggtcggcg cgcatgagcg gtggcgggcg 360
gaggtagcca agcttgtcgg ggacggtgtt cctgaggagc aggctcgtag gcaggtgcgc 420
gtcccggttc cgatgaagcc ggcgatccag aaggcgttga acgcggtcaa gggtgactct 480
cgcaagggct tggatggtgc gtgcccgtgg tggcatgagg tcaacactta cgcgttccag 540
tccgctttca tcgacgcgga ccaggcgtgg aagaactggt tggactcgtt gtcggggaag 600
cgggtcggcc gccgggtggg gtacccgcgg ttcaagaaga aggggcgggc ccgcgactcg 660
tttcgcttgc accacgacgt gaagaagcca agcatccggc tggccgggta ccggcggttg 720
cggctgccga ggatcgggga agtccggctt catgactcgg ggaagcgtct ggcacggctg 780
atcgaccgag gtgacgcggt ggtgcagtcg gtgacggtgt cgcggggtgg ccatcgctgg 840
tatgcgtctg tgctgtgcaa ggtcaccgtg caggtaccgg atcggccgtc acggcgccag 900
cgggaacgag gcgctgttgg ggtggacctt ggcgttaagg tcctcgcggc tttgtccaag 960
ccactggtgg tcgacgaccc gtcgagtgcc ctggtgcgga atccgcagca tctgcggcag 1020
gccgagcggc gtctactgaa ggctcagcgg gcgttggcgc gtacacaaaa aggttcagcc 1080
cgacgcgaga aggccaagcg gcgagttggt cgcgcgcatc acgaggttgc tgtgcgccgg 1140
catgctgcgc tccatcagat caccaagcgc ctgaccaccg gcttcgcggt cgtcgcgctt 1200
gaggatctga acgtggccgg tatgacaagg tcagcgcggg gaacagtggc agcgccggga 1260
aagaacgtgc ggcagaaggc aggcctgaac cgcgtcatcc tggactccgc tccagccgag 1320
ttgcgtcgcc aggtgaacta caaggccacc tggtatggat ctgagctggc ggtcgcagac 1380
cgctggttcc cctcgagtaa gacctgttcg ggttgcggat ggcaaaaccc acacctgaag 1440
ctgtccgaca gggtgtttcg ttgcacagac tgcgggctgg tgatggaccg ggacatgaac 1500
gcggcccgga acatcgaacg ccacgcagtg ctgatcgatc gaaacgtcgc ctgcgatagg 1560
cgggagacgc taaacgcccg tggagcatcc ataagaccaa ccacctctcg gggcggtagg 1620
cacgacgcat cgaagcggga aggctccggc gctcggcctg agtcacctca gcagagtgat 1680
ctgctgacgc tcccaacctt gaataacgaa acggcaacgc ctccatagcg gtgcaggtca 1740
ataagggtcg gccccacgcg tgtagggagc gatcgcgaga ccattggtct caggccggca 1800
tggtcccagc ctcctcgctg gcgccggctg ggcaacatgc ttcggcatgg cgaatgggac 1860
ccaattattg aacaccctaa cgggtgtttt tttgtttctg gtctacctgg ctggttggcg 1920
tactgttgtg gtaaataccc atatggacag tt 1952
<210> 126
<211> 6296
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 126
gtggtaaata cccatatgga cagttttccc tttgatatgt aacggtgaac agttgttcta 60
cttttgtttg ttagtcttga tgcttcactg atagatacaa gagcgtcggg tgatgctgcc 120
aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat 180
cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg 240
acatcagcgc tagcggagtg tatactggct tactatgttg gcactgatga gggtgtcagt 300
gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat 360
acaggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc 420
gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta 480
acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa 540
gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata 600
ccaggcgttt ccccctggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac 660
cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg 720
ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct 780
gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacatgca aaagcaccac 840
tggcagcagc cactggtaat tgatttagag gagttagtct tgaagtcatg cgccggttaa 900
ggctaaactg aaaggacaag ttttggtgac tgcgctcctc caagccagtt acctcggttc 960
aaagagttgg tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt 1020
tcagagcaag agattacgcg cagaccaaaa cgatctcaag aagatcatct tattaatcag 1080
ataaaatatt tctagatttc agtgcaattt atctcttcaa atgtagcacc tgaagtcagc 1140
cccatacgat ataagttgtg acgtaaaatg agacgttgat cggcacgtaa gaggttccaa 1200
ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc 1260
aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc 1320
ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa 1380
ccagaccgtt cagctgggtc atccaccgga tcaattcccc tgctcgcgca ggctgggtgc 1440
caagctctcg ggtaacatca aggcccgatc cttggagccc ttgccctccc gcacgatgat 1500
cgtgccgtga tcgaaatcca gatccttgac ccgcagttgc aaaccctcac tgatccggct 1560
cacggtaact gatgccgtat ttgcagtacc agcgtacggc ccacagaatg atgtcacgct 1620
gaaaatgccg gcctttgaat gggttcatgt gcagctccat cagcaaaagg ggatgataag 1680
tttatcacca ccgactattt gcaacagtgc cgttgatcgt gctatgatcg actgatgtca 1740
tcagcggtgg agtgcaatgt cgtgcaatac gaatggcgaa aagccgagct catcggtcag 1800
cttctcaacc ttggggttac ccccggcggt gtgctgctgg tccacagctc cttccgtagc 1860
gtccggcccc tcgaagatgg gccacttgga ctgatcgagg ccctgcgtgc tgcgctgggt 1920
ccgggaggga cgctcgtcat gccctcgtgg tcaggtctgg acgacgagcc gttcgatcct 1980
gccacgtcgc ccgttacacc ggaccttgga gttgtctctg acacattctg gcgcctgcca 2040
aatgtaaagc gcagcgccca tccatttgcc tttgcggcag cggggccaca ggcagagcag 2100
atcatctctg atccattgcc cctgccacct cactcgcctg caagcccggt cgcccgtgtc 2160
catgaactcg atgggcaggt acttctcctc ggcgtgggac acgatgccaa cacgacgctg 2220
catcttgccg agttgatggc aaaggttccc tatggggtgc cgagacactg caccattctt 2280
caggatggca agttggtacg cgtcgattat ctcgagaatg accactgctg tgagcgcttt 2340
gccttggcgg acaggtggct caaggagaag agccttcaga aggaaggtcc agtcggtcat 2400
gcctttgctc ggttgatccg ctcccgcgac attgtggcga cagccctggg tcaactgggc 2460
cgagatccgt tgatcttcct gcatccgcca gaggcgggat gcgaagaatg cgatgccgct 2520
cgccagtcga ttggctgagc tcatgagcgg agaacgagat gacgttggag gggcaaggtc 2580
gcgctgattg ctggggcaac acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg 2640
tctaacggta cccggggatc ctctagagtc gacctgcata atgtgcctgt caaatggacg 2700
aagcagggat tctgcaaacc ctatgctact ccgtcaagcc gtcaattgtc tgattcgtta 2760
ccaattatga caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact 2820
cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa 2880
aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg 2940
cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga 3000
aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa 3060
aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca 3120
tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca 3180
gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc 3240
caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg 3300
caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc 3360
actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg 3420
ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc 3480
cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa 3540
aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa 3600
acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc 3660
cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt 3720
actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa 3780
gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag 3840
tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa 3900
gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt 3960
ttttttgggc tagcgaaaag atgtttcgtg aagccgtcga cgcttataaa aaatggatat 4020
taatactgaa actgagatca agcaaaagca ttcactaacc ccctttcctg ttttcctaat 4080
cagcccggca tttcgcgggc gatattttca cagctatttc aggagttcag ccatgaacgc 4140
ttattacatt caggatcgtc ttgaggctca gagctgggcg cgtcactacc agcagctcgc 4200
ccgtgaagag aaagaggcag aactggcaga cgacatggaa aaaggcctgc cccagcacct 4260
gtttgaatcg ctatgcatcg atcatttgca acgccacggg gccagcaaaa aatccattac 4320
ccgtgcgttt gatgacgatg ttgagtttca ggagcgcatg gcagaacaca tccggtacat 4380
ggttgaaacc attgctcacc accaggttga tattgattca gaggtataaa acgaatgagt 4440
actgcactcg caacgctggc tgggaagctg gctgaacgtg tcggcatgga ttctgtcgac 4500
ccacaggaac tgatcaccac tcttcgccag acggcattta aaggtgatgc cagcgatgcg 4560
cagttcatcg cattactgat cgttgccaac cagtacggcc ttaatccgtg gacgaaagaa 4620
atttacgcct ttcctgataa gcagaatggc atcgttccgg tggtgggcgt tgatggctgg 4680
tcccgcatca tcaatgaaaa ccagcagttt gatggcatgg actttgagca ggacaatgaa 4740
tcctgtacat gccggattta ccgcaaggac cgtaatcatc cgatctgcgt taccgaatgg 4800
atggatgaat gccgccgcga accattcaaa actcgcgaag gcagagaaat cacggggccg 4860
tggcagtcgc atcccaaacg gatgttacgt cataaagcca tgattcagtg tgcccgtctg 4920
gccttcggat ttgctggtat ctatgacaag gatgaagccg agcgcattgt cgaaaatact 4980
gcatacactg cagaacgtca gccggaacgc gacatcactc cggttaacga tgaaaccatg 5040
caggagatta acactctgct gatcgccctg gataaaacat gggatgacga cttattgccg 5100
ctctgttccc agatatttcg ccgcgacatt cgtgcatcgt cagaactgac acaggccgaa 5160
gcagtaaaag ctcttggatt cctgaaacag aaagccgcag agcagaaggt ggcagcatga 5220
caccggacat tatcctgcag cgtaccggga tcgatgtgag agctgtcgaa cagggggatg 5280
atgcgtggca caaattacgg ctcggcgtca tcaccgcttc agaagttcac aacgtgatag 5340
caaaaccccg ctccggaaag aagtggcctg acatgaaaat gtcctacttc cacaccctgc 5400
ttgctgaggt ttgcaccggt gtggctccgg aagttaacgc taaagcactg gcctggggaa 5460
aacagtacga gaacgacgcc agaaccctgt ttgaattcac ttccggcgtg aatgttactg 5520
aatccccgat catctatcgc gacgaaagta tgcgtaccgc ctgctctccc gatggtttat 5580
gcagtgacgg caacggcctt gaactgaaat gcccgtttac ctcccgggat ttcatgaagt 5640
tccggctcgg tggtttcgag gccataaagt cagcttacat ggcccaggtg cagtacagca 5700
tgtgggtgac gcgaaaaaat gcctggtact ttgccaacta tgacccgcgt atgaagcgtg 5760
aaggcctgca ttatgtcgtg attgagcggg atgaaaagta catggcgagt tttgacgaga 5820
tcgtgccgga gttcatcgaa aaaatggacg aggcactggc tgaaattggt tttgtatttg 5880
gggagcaatg gcgatgacgc atcctcacga taataagctt cctgctgaac atcaaaggca 5940
agaaaacatc tgttgtcaaa gacagcatcc ttgaacaagg acaattaaca gttaacaaat 6000
aaaaacgcaa aagaaaatgc cgatattgac taccggaagc agtgtgaccg tgtgcttctc 6060
aaatgcctga ttcaggctgt ctatgtgtga ctgttgagct gtaacaagtt gtctcaggtg 6120
ttcaatttca tgttctagtt gctttgtttt actggtttca cctgttctat taggtgttac 6180
atgctgttca tctgttacat tgtcgatctg ttcatggtga acagctttaa atgcaccaaa 6240
aactcgtaaa agctctgatg tatctatctt ttttacaccg ttttcatctg tgcata 6296
<210> 127
<211> 8201
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 127
tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg 60
gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg 120
ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc 180
tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc 240
acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 300
acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag 360
ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc 420
gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa 480
ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga 540
tctcaagaag atcatcttat taatcagata aaatatttct agatttcagt gcaatttatc 600
tcttcaaatg tagcacctga agtcagcccc atacgatata agttgtgacg taaaatgaga 660
cgttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg 720
cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat 780
cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt 840
tcagtcagtt gctcaatgta cctataacca gaccgttcag ctgggtcatc caccggatca 900
attcccctgc tcgcgcaggc tgggtgccaa gctctcgggt aacatcaagg cccgatcctt 960
ggagcccttg ccctcccgca cgatgatcgt gccgtgatcg aaatccagat ccttgacccg 1020
cagttgcaaa ccctcactga tccggctcac ggtaactgat gccgtatttg cagtaccagc 1080
gtacggccca cagaatgatg tcacgctgaa aatgccggcc tttgaatggg ttcatgtgca 1140
gctccatcag caaaagggga tgataagttt atcaccaccg actatttgca acagtgccgt 1200
tgatcgtgct atgatcgact gatgtcatca gcggtggagt gcaatgtcgt gcaatacgaa 1260
tggcgaaaag ccgagctcat cggtcagctt ctcaaccttg gggttacccc cggcggtgtg 1320
ctgctggtcc acagctcctt ccgtagcgtc cggcccctcg aagatgggcc acttggactg 1380
atcgaggccc tgcgtgctgc gctgggtccg ggagggacgc tcgtcatgcc ctcgtggtca 1440
ggtctggacg acgagccgtt cgatcctgcc acgtcgcccg ttacaccgga ccttggagtt 1500
gtctctgaca cattctggcg cctgccaaat gtaaagcgca gcgcccatcc atttgccttt 1560
gcggcagcgg ggccacaggc agagcagatc atctctgatc cattgcccct gccacctcac 1620
tcgcctgcaa gcccggtcgc ccgtgtccat gaactcgatg ggcaggtact tctcctcggc 1680
gtgggacacg atgccaacac gacgctgcat cttgccgagt tgatggcaaa ggttccctat 1740
ggggtgccga gacactgcac cattcttcag gatggcaagt tggtacgcgt cgattatctc 1800
gagaatgacc actgctgtga gcgctttgcc ttggcggaca ggtggctcaa ggagaagagc 1860
cttcagaagg aaggtccagt cggtcatgcc tttgctcggt tgatccgctc ccgcgacatt 1920
gtggcgacag ccctgggtca actgggccga gatccgttga tcttcctgca tccgccagag 1980
gcgggatgcg aagaatgcga tgccgctcgc cagtcgattg gctgagctca tgagcggaga 2040
acgagatgac gttggagggg caaggtcgcg ctgattgctg gggcaacacg tgaaaggcga 2100
gatcaccaag gtagtcggca aataatgtct aacggtaccc ggggatcctc tagagtcgac 2160
ctgcataatg tgcctgtcaa atggacgaag cagggattct gcaaacccta tgctactccg 2220
tcaagccgtc aattgtctga ttcgttacca attatgacaa cttgacggct acatcattca 2280
ctttttcttc acaaccggca cggaactcgc tcgggctggc cccggtgcat tttttaaata 2340
cccgcgagaa atagagttga tcgtcaaaac caacattgcg accgacggtg gcgataggca 2400
tccgggtggt gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg cgccagctta 2460
agacgctaat ccctaactgc tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa 2520
catgctgtgc gacgctggcg atatcaaaat tgctgtctgc caggtgatcg ctgatgtact 2580
gacaagcctc gcgtacccga ttatccatcg gtggatggag cgactcgtta atcgcttcca 2640
tgcgccgcag taacaattgc tcaagcagat ttatcgccag cagctccgaa tagcgccctt 2700
ccccttgccc ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt 2760
catccgggcg aaagaacccc gtattggcaa atattgacgg ccagttaagc cattcatgcc 2820
agtaggcgcg cggacgaaag taaacccact ggtgatacca ttcgcgagcc tccggatgac 2880
gaccgtagtg atgaatctct cctggcggga acagcaaaat atcacccggt cggcaaacaa 2940
attctcgtcc ctgatttttc accaccccct gaccgcgaat ggtgagattg agaatataac 3000
ctttcattcc cagcggtcgg tcgataaaaa aatcgagata accgttggcc tcaatcggcg 3060
ttaaacccgc caccagatgg gcattaaacg agtatcccgg cagcagggga tcattttgcg 3120
cttcagccat acttttcata ctcccgccat tcagagaaga aaccaattgt ccatattgca 3180
tcagacattg ccgtcactgc gtcttttact ggctcttctc gctaaccaaa ccggtaaccc 3240
cgcttattaa aagcattctg taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca 3300
aaagtgtcta taatcacggc agaaaagtcc acattgatta tttgcacggc gtcacacttt 3360
gctatgccat agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc 3420
aactctctac tgtttctcca tacccgtttt tttgggctag cgaaaagatg tttcgtgaag 3480
ccgtcgacgc ttataaaaaa tggatattaa tactgaaact gagatcaagc aaaagcattc 3540
actaaccccc tttcctgttt tcctaatcag cccggcattt cgcgggcgat attttcacag 3600
ctatttcagg agttcagcca tgaacgctta ttacattcag gatcgtcttg aggctcagag 3660
ctgggcgcgt cactaccagc agctcgcccg tgaagagaaa gaggcagaac tggcagacga 3720
catggaaaaa ggcctgcccc agcacctgtt tgaatcgcta tgcatcgatc atttgcaacg 3780
ccacggggcc agcaaaaaat ccattacccg tgcgtttgat gacgatgttg agtttcagga 3840
gcgcatggca gaacacatcc ggtacatggt tgaaaccatt gctcaccacc aggttgatat 3900
tgattcagag gtataaaacg aatgagtact gcactcgcaa cgctggctgg gaagctggct 3960
gaacgtgtcg gcatggattc tgtcgaccca caggaactga tcaccactct tcgccagacg 4020
gcatttaaag gtgatgccag cgatgcgcag ttcatcgcat tactgatcgt tgccaaccag 4080
tacggcctta atccgtggac gaaagaaatt tacgcctttc ctgataagca gaatggcatc 4140
gttccggtgg tgggcgttga tggctggtcc cgcatcatca atgaaaacca gcagtttgat 4200
ggcatggact ttgagcagga caatgaatcc tgtacatgcc ggatttaccg caaggaccgt 4260
aatcatccga tctgcgttac cgaatggatg gatgaatgcc gccgcgaacc attcaaaact 4320
cgcgaaggca gagaaatcac ggggccgtgg cagtcgcatc ccaaacggat gttacgtcat 4380
aaagccatga ttcagtgtgc ccgtctggcc ttcggatttg ctggtatcta tgacaaggat 4440
gaagccgagc gcattgtcga aaatactgca tacactgcag aacgtcagcc ggaacgcgac 4500
atcactccgg ttaacgatga aaccatgcag gagattaaca ctctgctgat cgccctggat 4560
aaaacatggg atgacgactt attgccgctc tgttcccaga tatttcgccg cgacattcgt 4620
gcatcgtcag aactgacaca ggccgaagca gtaaaagctc ttggattcct gaaacagaaa 4680
gccgcagagc agaaggtggc agcatgacac cggacattat cctgcagcgt accgggatcg 4740
atgtgagagc tgtcgaacag ggggatgatg cgtggcacaa attacggctc ggcgtcatca 4800
ccgcttcaga agttcacaac gtgatagcaa aaccccgctc cggaaagaag tggcctgaca 4860
tgaaaatgtc ctacttccac accctgcttg ctgaggtttg caccggtgtg gctccggaag 4920
ttaacgctaa agcactggcc tggggaaaac agtacgagaa cgacgccaga accctgtttg 4980
aattcacttc cggcgtgaat gttactgaat ccccgatcat ctatcgcgac gaaagtatgc 5040
gtaccgcctg ctctcccgat ggtttatgca gtgacggcaa cggccttgaa ctgaaatgcc 5100
cgtttacctc ccgggatttc atgaagttcc ggctcggtgg tttcgaggcc ataaagtcag 5160
cttacatggc ccaggtgcag tacagcatgt gggtgacgcg aaaaaatgcc tggtactttg 5220
ccaactatga cccgcgtatg aagcgtgaag gcctgcatta tgtcgtgatt gagcgggatg 5280
aaaagtacat ggcgagtttt gacgagatcg tgccggagtt catcgaaaaa atggacgagg 5340
cactggctga aattggtttt gtatttgggg agcaatggcg atgacgcatc ctcacgataa 5400
taagcttcct gctgaacatc aaaggcaaga aaacatctgt tgtcaaagac agcatccttg 5460
aacaaggaca attaacagtt aacaaataaa aacgcaaaag aaaatgccga tattgactac 5520
cggaagcagt gtgaccgtgt gcttctcaaa tgcctgattc aggctgtcta tgtgtgactg 5580
ttgagctgta acaagttgtc tcaggtgttc aatttcatgt tctagttgct ttgttttact 5640
ggtttcacct gttctattag gtgttacatg ctgttcatct gttacattgt cgatctgttc 5700
atggtgaaca gctttaaatg caccaaaaac tcgtaaaagc tctgatgtat ctatcttttt 5760
tacaccgttt tcatctgtgc atatagctat actgatttcg tcagactcac agtcaaacat 5820
gccggtcagt tggcctggtg atggcgggat cgttgtatat ttcttgacac cttttcggca 5880
ccgccctaaa attctgcgtc ctcataatat atgaggcgat ttattacgtg tttacgaagc 5940
aaaagctaaa accaggagct atttaatggt gcagacggag atcctgaagg cgtttcggtt 6000
tgcgttggat ccgacgtctg tgcaggttgc tgccctttcc cggcatgctg gtgcggccag 6060
gtgggcgttc aaccatgctc tcgcggccaa ggtcggcgcg catgagcggt ggcgggcgga 6120
ggtagccaag cttgtcgggg acggtgttcc tgaggagcag gctcgtaggc aggtgcgcgt 6180
cccggttccg atgaagccgg cgatccagaa ggcgttgaac gcggtcaagg gtgactctcg 6240
caagggcttg gatggtgcgt gcccgtggtg gcatgaggtc aacacttacg cgttccagtc 6300
cgctttcatc gacgcggacc aggcgtggaa gaactggttg gactcgttgt cggggaagcg 6360
ggtcggccgc cgggtggggt acccgcggtt caagaagaag gggcgggccc gcgactcgtt 6420
tcgcttgcac cacgacgtga agaagccaag catccggctg gccgggtacc ggcggttgcg 6480
gctgccgagg atcggggaag tccggcttca tgactcgggg aagcgtctgg cacggctgat 6540
cgaccgaggt gacgcggtgg tgcagtcggt gacggtgtcg cggggtggcc atcgctggta 6600
tgcgtctgtg ctgtgcaagg tcaccgtgca ggtaccggat cggccgtcac ggcgccagcg 6660
ggaacgaggc gctgttgggg tggaccttgg cgttaaggtc ctcgcggctt tgtccaagcc 6720
actggtggtc gacgacccgt cgagtgccct ggtgcggaat ccgcagcatc tgcggcaggc 6780
cgagcggcgt ctactgaagg ctcagcgggc gttggcgcgt acacaaaaag gttcagcccg 6840
acgcgagaag gccaagcggc gagttggtcg cgcgcatcac gaggttgctg tgcgccggca 6900
tgctgcgctc catcagatca ccaagcgcct gaccaccggc ttcgcggtcg tcgcgcttga 6960
ggatctgaac gtggccggta tgacaaggtc agcgcgggga acagtggcag cgccgggaaa 7020
gaacgtgcgg cagaaggcag gcctgaaccg cgtcatcctg gactccgctc cagccgagtt 7080
gcgtcgccag gtgaactaca aggccacctg gtatggatct gagctggcgg tcgcagaccg 7140
ctggttcccc tcgagtaaga cctgttcggg ttgcggatgg caaaacccac acctgaagct 7200
gtccgacagg gtgtttcgtt gcacagactg cgggctggtg atggaccggg acatgaacgc 7260
ggcccggaac atcgaacgcc acgcagtgct gatcgatcga aacgtcgcct gcgataggcg 7320
ggagacgcta aacgcccgtg gagcatccat aagaccaacc acctctcggg gcggtaggca 7380
cgacgcatcg aagcgggaag gctccggcgc tcggcctgag tcacctcagc agagtgatct 7440
gctgacgctc ccaaccttga ataacgaaac ggcaacgcct ccatagcggt gcaggtcaat 7500
aagggtcggc cccacgcgtg tagggagcga tcgtgtcctc ttcctcttta gcgggccggc 7560
atggtcccag cctcctcgct ggcgccggct gggcaacatg cttcggcatg gcgaatggga 7620
cccaattatt gaacacccta acgggtgttt ttttgtttct ggtctacctg gctggttggc 7680
gtactgttgt ggtaaatacc catatggaca gttttccctt tgatatgtaa cggtgaacag 7740
ttgttctact tttgtttgtt agtcttgatg cttcactgat agatacaaga gcgtcgggtg 7800
atgctgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc agtggcttct 7860
gtttctatca gctgtccctc ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc 7920
accgccggac atcagcgcta gcggagtgta tactggctta ctatgttggc actgatgagg 7980
gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc gtcagcagaa 8040
tatgtgatac aggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg 8100
actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag atgccaggaa 8160
gatacttaac agggaagtga gagggccgcg gcaaagccgt t 8201
<210> 128
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 128
atcgtgtcct cttcctcttt agcg 24
<210> 129
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 129
ggcccgctaa agaggaagag gaca 24
<210> 130
<211> 2707
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 130
gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 60
tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 120
cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 180
gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 240
gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 300
gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 360
ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 420
atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 480
tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 540
ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 600
gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 660
ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 720
ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 780
agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 840
ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 900
aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 960
tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 1020
cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 1080
tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 1140
cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 1200
ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 1260
gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 1320
gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 1380
gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 1440
gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 1500
tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 1560
aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 1620
cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 1680
caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 1740
cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 1800
ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 1860
aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 1920
tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 1980
tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct 2040
ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 2100
cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 2160
cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga 2220
gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca 2280
ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt 2340
cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc 2400
cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcgac aaattctaaa 2460
cgctaaagag gaagaggaca nnnnnnagtt gacccaacgt cgccggcgtg cacaagcttg 2520
gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 2580
aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 2640
acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 2700
cattaat 2707
<210> 131
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 131
ccggcgacgt tgggtcaact nnnnnntgtc ctcttcctct ttagcg 46
<210> 132
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 132
agttgaccca acgtcgccgg cgtgcacaag 30
<210> 133
<211> 51
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 133
gtgactggag ttcagacgtg tgctcttccg atctcaggaa acagctatga c 51
<210> 134
<211> 43
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 134
gttcagagtt ctacagtccg acgatcgtaa aacgacggcc agt 43
<210> 135
<211> 66
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 135
caagcagaag acggcatacg agatcggttc aagtgactgg agttcagacg tgtgctcttc 60
cgatct 66
<210> 136
<211> 63
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 136
aatgatacgg cgaccaccga gatctacacg ctggattgtt cagagttcta cagtccgacg 60
atc 63
<210> 137
<211> 8037
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 137
tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg 60
gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg 120
ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc 180
tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc 240
acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 300
acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag 360
ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc 420
gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa 480
ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga 540
tctcaagaag atcatcttat taatcagata aaatatttct agatttcagt gcaatttatc 600
tcttcaaatg tagcacctga agtcagcccc atacgatata agttgtgacg taaaatgaga 660
cgttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg 720
cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat 780
cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt 840
tcagtcagtt gctcaatgta cctataacca gaccgttcag ctgggtcatc caccggatca 900
attcccctgc tcgcgcaggc tgggtgccaa gctctcgggt aacatcaagg cccgatcctt 960
ggagcccttg ccctcccgca cgatgatcgt gccgtgatcg aaatccagat ccttgacccg 1020
cagttgcaaa ccctcactga tccggctcac ggtaactgat gccgtatttg cagtaccagc 1080
gtacggccca cagaatgatg tcacgctgaa aatgccggcc tttgaatggg ttcatgtgca 1140
gctccatcag caaaagggga tgataagttt atcaccaccg actatttgca acagtgccgt 1200
tgatcgtgct atgatcgact gatgtcatca gcggtggagt gcaatgtcgt gcaatacgaa 1260
tggcgaaaag ccgagctcat cggtcagctt ctcaaccttg gggttacccc cggcggtgtg 1320
ctgctggtcc acagctcctt ccgtagcgtc cggcccctcg aagatgggcc acttggactg 1380
atcgaggccc tgcgtgctgc gctgggtccg ggagggacgc tcgtcatgcc ctcgtggtca 1440
ggtctggacg acgagccgtt cgatcctgcc acgtcgcccg ttacaccgga ccttggagtt 1500
gtctctgaca cattctggcg cctgccaaat gtaaagcgca gcgcccatcc atttgccttt 1560
gcggcagcgg ggccacaggc agagcagatc atctctgatc cattgcccct gccacctcac 1620
tcgcctgcaa gcccggtcgc ccgtgtccat gaactcgatg ggcaggtact tctcctcggc 1680
gtgggacacg atgccaacac gacgctgcat cttgccgagt tgatggcaaa ggttccctat 1740
ggggtgccga gacactgcac cattcttcag gatggcaagt tggtacgcgt cgattatctc 1800
gagaatgacc actgctgtga gcgctttgcc ttggcggaca ggtggctcaa ggagaagagc 1860
cttcagaagg aaggtccagt cggtcatgcc tttgctcggt tgatccgctc ccgcgacatt 1920
gtggcgacag ccctgggtca actgggccga gatccgttga tcttcctgca tccgccagag 1980
gcgggatgcg aagaatgcga tgccgctcgc cagtcgattg gctgagctca tgagcggaga 2040
acgagatgac gttggagggg caaggtcgcg ctgattgctg gggcaacacg tgaaaggcga 2100
gatcaccaag gtagtcggca aataatgtct aacggtaccc ggggatcctc tagagtcgac 2160
ctgcataatg tgcctgtcaa atggacgaag cagggattct gcaaacccta tgctactccg 2220
tcaagccgtc aattgtctga ttcgttacca attatgacaa cttgacggct acatcattca 2280
ctttttcttc acaaccggca cggaactcgc tcgggctggc cccggtgcat tttttaaata 2340
cccgcgagaa atagagttga tcgtcaaaac caacattgcg accgacggtg gcgataggca 2400
tccgggtggt gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg cgccagctta 2460
agacgctaat ccctaactgc tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa 2520
catgctgtgc gacgctggcg atatcaaaat tgctgtctgc caggtgatcg ctgatgtact 2580
gacaagcctc gcgtacccga ttatccatcg gtggatggag cgactcgtta atcgcttcca 2640
tgcgccgcag taacaattgc tcaagcagat ttatcgccag cagctccgaa tagcgccctt 2700
ccccttgccc ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt 2760
catccgggcg aaagaacccc gtattggcaa atattgacgg ccagttaagc cattcatgcc 2820
agtaggcgcg cggacgaaag taaacccact ggtgatacca ttcgcgagcc tccggatgac 2880
gaccgtagtg atgaatctct cctggcggga acagcaaaat atcacccggt cggcaaacaa 2940
attctcgtcc ctgatttttc accaccccct gaccgcgaat ggtgagattg agaatataac 3000
ctttcattcc cagcggtcgg tcgataaaaa aatcgagata accgttggcc tcaatcggcg 3060
ttaaacccgc caccagatgg gcattaaacg agtatcccgg cagcagggga tcattttgcg 3120
cttcagccat acttttcata ctcccgccat tcagagaaga aaccaattgt ccatattgca 3180
tcagacattg ccgtcactgc gtcttttact ggctcttctc gctaaccaaa ccggtaaccc 3240
cgcttattaa aagcattctg taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca 3300
aaagtgtcta taatcacggc agaaaagtcc acattgatta tttgcacggc gtcacacttt 3360
gctatgccat agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc 3420
aactctctac tgtttctcca tacccgtttt tttgggctag cgaaaagatg tttcgtgaag 3480
ccgtcgacgc ttataaaaaa tggatattaa tactgaaact gagatcaagc aaaagcattc 3540
actaaccccc tttcctgttt tcctaatcag cccggcattt cgcgggcgat attttcacag 3600
ctatttcagg agttcagcca tgaacgctta ttacattcag gatcgtcttg aggctcagag 3660
ctgggcgcgt cactaccagc agctcgcccg tgaagagaaa gaggcagaac tggcagacga 3720
catggaaaaa ggcctgcccc agcacctgtt tgaatcgcta tgcatcgatc atttgcaacg 3780
ccacggggcc agcaaaaaat ccattacccg tgcgtttgat gacgatgttg agtttcagga 3840
gcgcatggca gaacacatcc ggtacatggt tgaaaccatt gctcaccacc aggttgatat 3900
tgattcagag gtataaaacg aatgagtact gcactcgcaa cgctggctgg gaagctggct 3960
gaacgtgtcg gcatggattc tgtcgaccca caggaactga tcaccactct tcgccagacg 4020
gcatttaaag gtgatgccag cgatgcgcag ttcatcgcat tactgatcgt tgccaaccag 4080
tacggcctta atccgtggac gaaagaaatt tacgcctttc ctgataagca gaatggcatc 4140
gttccggtgg tgggcgttga tggctggtcc cgcatcatca atgaaaacca gcagtttgat 4200
ggcatggact ttgagcagga caatgaatcc tgtacatgcc ggatttaccg caaggaccgt 4260
aatcatccga tctgcgttac cgaatggatg gatgaatgcc gccgcgaacc attcaaaact 4320
cgcgaaggca gagaaatcac ggggccgtgg cagtcgcatc ccaaacggat gttacgtcat 4380
aaagccatga ttcagtgtgc ccgtctggcc ttcggatttg ctggtatcta tgacaaggat 4440
gaagccgagc gcattgtcga aaatactgca tacactgcag aacgtcagcc ggaacgcgac 4500
atcactccgg ttaacgatga aaccatgcag gagattaaca ctctgctgat cgccctggat 4560
aaaacatggg atgacgactt attgccgctc tgttcccaga tatttcgccg cgacattcgt 4620
gcatcgtcag aactgacaca ggccgaagca gtaaaagctc ttggattcct gaaacagaaa 4680
gccgcagagc agaaggtggc agcatgacac cggacattat cctgcagcgt accgggatcg 4740
atgtgagagc tgtcgaacag ggggatgatg cgtggcacaa attacggctc ggcgtcatca 4800
ccgcttcaga agttcacaac gtgatagcaa aaccccgctc cggaaagaag tggcctgaca 4860
tgaaaatgtc ctacttccac accctgcttg ctgaggtttg caccggtgtg gctccggaag 4920
ttaacgctaa agcactggcc tggggaaaac agtacgagaa cgacgccaga accctgtttg 4980
aattcacttc cggcgtgaat gttactgaat ccccgatcat ctatcgcgac gaaagtatgc 5040
gtaccgcctg ctctcccgat ggtttatgca gtgacggcaa cggccttgaa ctgaaatgcc 5100
cgtttacctc ccgggatttc atgaagttcc ggctcggtgg tttcgaggcc ataaagtcag 5160
cttacatggc ccaggtgcag tacagcatgt gggtgacgcg aaaaaatgcc tggtactttg 5220
ccaactatga cccgcgtatg aagcgtgaag gcctgcatta tgtcgtgatt gagcgggatg 5280
aaaagtacat ggcgagtttt gacgagatcg tgccggagtt catcgaaaaa atggacgagg 5340
cactggctga aattggtttt gtatttgggg agcaatggcg atgacgcatc ctcacgataa 5400
taagcttcct gctgaacatc aaaggcaaga aaacatctgt tgtcaaagac agcatccttg 5460
aacaaggaca attaacagtt aacaaataaa aacgcaaaag aaaatgccga tattgactac 5520
cggaagcagt gtgaccgtgt gcttctcaaa tgcctgattc aggctgtcta tgtgtgactg 5580
ttgagctgta acaagttgtc tcaggtgttc aatttcatgt tctagttgct ttgttttact 5640
ggtttcacct gttctattag gtgttacatg ctgttcatct gttacattgt cgatctgttc 5700
atggtgaaca gctttaaatg caccaaaaac tcgtaaaagc tctgatgtat ctatcttttt 5760
tacaccgttt tcatctgtgc atatagctat actgatttcg tcagactcac agtcaaacat 5820
gccggtcagt tggcctggtg atggcgggat cgttgtatat ttcttgacac cttttcggca 5880
ccgccctaaa attctgcgtc ctcataatat atgaggcgat ttattacgtg tttacgaagc 5940
aaaagctaaa accaggagct atttaatggt gcagacggag atcctgaagg cgtttcggtt 6000
tgcgttggat ccgacgtctg tgcaggttgc tgccctttcc cggcatgctg gtgcggccag 6060
gtgggcgttc aaccatgctc tcgcggccaa ggtcggcgcg catgagcggt ggcgggcgga 6120
ggtagccaag cttgtcgggg acggtgttcc tgaggagcag gctcgtaggc aggtgcgcgt 6180
cccggttccg atgaagccgg cgatccagaa ggcgttgaac gcggtcaagg gtgactctcg 6240
caagggcttg gatggtgcgt gcccgtggtg gcatgaggtc aacacttacg cgttccagtc 6300
cgctttcatc gacgcggacc aggcgtggaa gaactggttg gactcgttgt cggggaagcg 6360
ggtcggccgc cgggtggggt acccgcggtt caagaagaag gggcgggccc gcgactcgtt 6420
tcgcttgcac cacgacgtga agaagccaag catccggctg gccgggtacc ggcggttgcg 6480
gctgccgagg atcggggaag tccggcttca tgactcgggg aagcgtctgg cacggctgat 6540
cgaccgaggt gacgcggtgg tgcagtcggt gacggtgtcg cggggtggcc atcgctggta 6600
tgcgtctgtg ctgtgcaagg tcaccgtgca ggtaccggat cggccgtcac ggcgccagcg 6660
ggaacgaggc gctgttgggg tggaccttgg cgttaaggtc ctcgcggctt tgtccaagcc 6720
actggtggtc gacgacccgt cgagtgccct ggtgcggaat ccgcagcatc tgcggcaggc 6780
cgagcggcgt ctactgaagg ctcagcgggc gttggcgcgt acacaaaaag gttcagcccg 6840
acgcgagaag gccaagcggc gagttggtcg cgcgcatcac gaggttgctg tgcgccggca 6900
tgctgcgctc catcagatca ccaagcgcct gaccaccggc ttcgcggtcg tcgcgcttga 6960
ggatctgaac gtggccggta tgacaaggtc agcgcgggga acagtggcag cgccgggaaa 7020
gaacgtgcgg cagaaggcag gcctgaaccg cgtcatcctg gactccgctc cagccgagtt 7080
gcgtcgccag gtgaactaca aggccacctg gtatggatct gagctggcgg tcgcagaccg 7140
ctggttcccc tcgagtaaga cctgttcggg ttgcggatgg caaaacccac acctgaagct 7200
gtccgacagg gtgtttcgtt gcacagactg cgggctggtg atggaccggg acatgaacgc 7260
ggcccggaac atcgaacgcc acgcagtgct gatcgatcga aacgtcgcct gcgataggcg 7320
ggagacgcta aacgcccgtg gagcatccat aagaccaacc acctctcggg gcggtaggca 7380
cgacgcatcg aagcgggaag gctccggcgc tcggcctgag tcacctcagc agagtgatct 7440
gctgacgctc ccaaccttga ataacgaaac ggcaacgcct ccatagcggt gcaggtcaat 7500
aaggtggctg gttggcgtac tgttgtggta aatacccata tggacagttt tccctttgat 7560
atgtaacggt gaacagttgt tctacttttg tttgttagtc ttgatgcttc actgatagat 7620
acaagagcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttgagg 7680
tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg acggggtggt 7740
gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact ggcttactat 7800
gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac 7860
cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc 7920
tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc 7980
tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgtt 8037
<210> 138
<211> 41
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 138
tcatctgtgc atatagctat actgatttcg tcagactcac a 41
<210> 139
<211> 36
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 139
cgccaaccag ccaccttatt gacctgcacc gctatg 36
<210> 140
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 140
caggtcaata aggtggctgg ttggcgtact gtt 33
<210> 141
<211> 43
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 141
atcagtatag ctatatgcac agatgaaaac ggtgtaaaaa aga 43
<210> 142
<211> 5040
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 142
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggtac 420
cgggcccccc ctcgaggtcg acggtatcga tacggcatca gagcagattg tactgagagt 480
gcaccataat cggcattttc ttttgcgttt ttatttgtta actgttaatt gtccttgttc 540
aaggatgctg tctttgacaa cagatgtttt cttgcctttg atgttcagca ggaagctagg 600
cgcaaacgtt gattgtttgt ctgcgtagaa tcctctgttt gtcatatagc ttgtaatcac 660
gacattgttt cctttcgctt gaggtacagc gaagtgtgag taagtaaagg ttacatcgtt 720
aggatcaaga tccattttta acacaaggcc agttttgttc agcggcttgt atgggccagt 780
taaagaatta gaaacataac caagcatgta aatatcgtta gacgtaatgc cgtcaatcgt 840
catttttgat ccgcgggagt cagtgaacag ataccatttg ccgttcattt taaagacgtt 900
cgcgcgttca atttcatctg ttactgtgtt agatgcaatc agcggtttca tcactttttt 960
cagtgtgtaa tcatcgttta gctcaatcat accgagagcg ccgtttgcta actcagccgt 1020
gcgtttttta tcgctttgca gaagtttttg actttcttga cggaagaatg atgtgctttt 1080
gccatagtat gctttgttaa ataaagattc ttcgccttgg tagccatctt cagttccagt 1140
gtttgcttca aatactaagt atttgtggcc tttatcttct acgtagtgag gatctctcag 1200
cgtatggttg tcgcctgagc tgtagttgcc ttcatcgatg aactgctgta cattttgata 1260
cgtttttccg tcaccgtcaa agattgattt ataatcctct acaccgttga tgttcaaaga 1320
gctgtctgat gctgatacgt taacttgtgc agttgtcagt gtttgtttgc cgtaatgttt 1380
accggagaaa tcagtgtaga ataaacggat ttttccgtca gatgtaaatg tggctgaacc 1440
tgaccattct tgtgtttggt cttttaggat agaatcattt gcatcgaatt tgtcgctgtc 1500
tttaaagacg cggccagcgt ttttccagct gtcaatagaa gtttcgccga ctttttgata 1560
gaacatgtaa atcgatgtgt catccgcatt tttaggatct ccggctaatg caaagacgat 1620
gtggtagccg tgatagtttg cgacagtgcc gtcagcgttt tgtaatggcc agctgtccca 1680
aacgtccagg ccttttgcag aagagatatt tttaattgtg gacgaatcga actcaggaac 1740
ttgatatttt tcattttttt gctgttcagg gatttgcagc atatcatggc gtgtaatatg 1800
ggaaatgccg tatgtttcct tatatggctt ttggttcgtt tctttcgcaa acgcttgagt 1860
tgcgcctcct gccagcagtg cggtagtaaa ggttaatact gttgcttgtt ttgcaaactt 1920
tttgatgttc atcgttcatg tctccttttt tatgtactgt gttagcggtc tgcttcttcc 1980
agccctcctg tttgaagatg gcaagttagt tacgcacaat aaaaaaagac ctaaaatatg 2040
taaggggtga cgccaaagta tacactttgc cctttacaca ttttaggtct tgcctgcttt 2100
atcagtaaca aacccgcgcg atttactttt cgacctcatt ctattagact ctcgtttgga 2160
ttgcaactgg tctattttcc tcttttgttt gatagaaaat cataaaagga tttgcagact 2220
acgggcctaa agaactaaaa aatctatctg tttcttttca ttctctgtat tttttatagt 2280
ttctgttgca tgggcataaa gttgcaagct tgatatcttg acagctagct cagtcctagg 2340
tataatacta gtgaacgcgg cccggaacat cgaacgccac gcagtgctga tcgatcgaaa 2400
cgtcgcctgc gataggcggg agacgctaaa cgcccgtgga gcatccataa gaccaaccac 2460
ctctcggggc ggtaggcacg acgcatcgaa gcgggaaggc tccggcgctc ggcctgagtc 2520
acctcagcag agtgatctgc tgacgctccc aaccttgaat aacgaaacgg caacgcctcc 2580
atagcggtgc aggtcaataa gggtcggccc cacgcgtgta gggagcgatc gcgagaccat 2640
tggtctcagg ccggcatggt cccagcctcc tcgctggcgc cggctgggca acatgcttcg 2700
gcatggcgaa tgggacccaa ttattgaaca ccctaacggg tgtttttttg tttctggtct 2760
accgatatcg aattcctgca gcccggggga tccactagtt ctagagcggc cgccaccgcg 2820
gtggagctca tcccaatggc gcgccgagct tggctcgagc atggtcatag ctgtttcctg 2880
tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 2940
aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 3000
ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 3060
gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 3120
tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 3180
aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 3240
gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 3300
aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 3360
ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 3420
tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 3480
tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 3540
ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 3600
tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 3660
ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 3720
tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 3780
aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 3840
aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 3900
aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 3960
ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 4020
acagttagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat 4080
caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt 4140
tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac 4200
aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga 4260
cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag 4320
gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg 4380
attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa 4440
tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag 4500
gatattcttc taatacctgg aatgctgttt tcccagggat cgcagtggtg agtaaccatg 4560
catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc 4620
agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca 4680
gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc 4740
cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc 4800
gcggcctaga gcaagacgtt tcccgttgaa tatggctcat actcttcctt tttcaatatt 4860
attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 4920
aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag 4980
aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc 5040
<210> 143
<211> 496
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 143
ggcataaagt tgcaagcttg atatcttgac agctagctca gtcctaggta taatactagt 60
gaacgcggcc cggaacatcg aacgccacgc agtgctgatc gatcgaaacg tcgcctgcga 120
taggcgggag acgctaaacg cccgtggagc atccataaga ccaaccacct ctcggggcgg 180
taggcacgac gcatcgaagc gggaaggctc cggcgctcgg cctgagtcac ctcagcagag 240
tgatctgctg acgctcccaa ccttgaataa cgaaacggca acgcctccat agcggtgcag 300
gtcaataagg gtcggcccca cgcgtgtagg gagcgatcgc gagaccattg gtctcaggcc 360
ggcatggtcc cagcctcctc gctggcgccg gctgggcaac atgcttcggc atggcgaatg 420
ggacccaatt attgaacacc ctaacgggtg tttttttgtt tctggtctac cgatatcgaa 480
ttcctgcagc ccgggg 496
<210> 144
<211> 4594
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 144
gatatcgaat tcctgcagcc cgggggatcc actagttcta gagcggccgc caccgcggtg 60
gagctcatcc caatggcgcg ccgagcttgg ctcgagcatg gtcatagctg tttcctgtgt 120
gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 180
cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 240
tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 300
gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 360
ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 420
caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 480
aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 540
atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 600
cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 660
ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 720
gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 780
accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 840
cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 900
cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 960
gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 1020
aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 1080
aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 1140
actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 1200
taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 1260
gttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa 1320
taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc 1380
ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac 1440
ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga 1500
ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc 1560
agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt 1620
gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg 1680
aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat 1740
attcttctaa tacctggaat gctgttttcc cagggatcgc agtggtgagt aaccatgcat 1800
catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt 1860
ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa 1920
acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga 1980
cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg 2040
gcctagagca agacgtttcc cgttgaatat ggctcatact cttccttttt caatattatt 2100
gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 2160
ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa 2220
ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg 2280
cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag 2340
cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg 2400
gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc 2460
atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt 2520
cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac 2580
gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt 2640
cccagtcacg acgttgtaaa acgacggcca gtgaattgac gcgtattggg atggtaccgg 2700
gccccccctc gaggtcgacg gtatcgatac ggcatcagag cagattgtac tgagagtgca 2760
ccataatcgg cattttcttt tgcgttttta tttgttaact gttaattgtc cttgttcaag 2820
gatgctgtct ttgacaacag atgttttctt gcctttgatg ttcagcagga agctaggcgc 2880
aaacgttgat tgtttgtctg cgtagaatcc tctgtttgtc atatagcttg taatcacgac 2940
attgtttcct ttcgcttgag gtacagcgaa gtgtgagtaa gtaaaggtta catcgttagg 3000
atcaagatcc atttttaaca caaggccagt tttgttcagc ggcttgtatg ggccagttaa 3060
agaattagaa acataaccaa gcatgtaaat atcgttagac gtaatgccgt caatcgtcat 3120
ttttgatccg cgggagtcag tgaacagata ccatttgccg ttcattttaa agacgttcgc 3180
gcgttcaatt tcatctgtta ctgtgttaga tgcaatcagc ggtttcatca cttttttcag 3240
tgtgtaatca tcgtttagct caatcatacc gagagcgccg tttgctaact cagccgtgcg 3300
ttttttatcg ctttgcagaa gtttttgact ttcttgacgg aagaatgatg tgcttttgcc 3360
atagtatgct ttgttaaata aagattcttc gccttggtag ccatcttcag ttccagtgtt 3420
tgcttcaaat actaagtatt tgtggccttt atcttctacg tagtgaggat ctctcagcgt 3480
atggttgtcg cctgagctgt agttgccttc atcgatgaac tgctgtacat tttgatacgt 3540
ttttccgtca ccgtcaaaga ttgatttata atcctctaca ccgttgatgt tcaaagagct 3600
gtctgatgct gatacgttaa cttgtgcagt tgtcagtgtt tgtttgccgt aatgtttacc 3660
ggagaaatca gtgtagaata aacggatttt tccgtcagat gtaaatgtgg ctgaacctga 3720
ccattcttgt gtttggtctt ttaggataga atcatttgca tcgaatttgt cgctgtcttt 3780
aaagacgcgg ccagcgtttt tccagctgtc aatagaagtt tcgccgactt tttgatagaa 3840
catgtaaatc gatgtgtcat ccgcattttt aggatctccg gctaatgcaa agacgatgtg 3900
gtagccgtga tagtttgcga cagtgccgtc agcgttttgt aatggccagc tgtcccaaac 3960
gtccaggcct tttgcagaag agatattttt aattgtggac gaatcgaact caggaacttg 4020
atatttttca tttttttgct gttcagggat ttgcagcata tcatggcgtg taatatggga 4080
aatgccgtat gtttccttat atggcttttg gttcgtttct ttcgcaaacg cttgagttgc 4140
gcctcctgcc agcagtgcgg tagtaaaggt taatactgtt gcttgttttg caaacttttt 4200
gatgttcatc gttcatgtct ccttttttat gtactgtgtt agcggtctgc ttcttccagc 4260
cctcctgttt gaagatggca agttagttac gcacaataaa aaaagaccta aaatatgtaa 4320
ggggtgacgc caaagtatac actttgccct ttacacattt taggtcttgc ctgctttatc 4380
agtaacaaac ccgcgcgatt tacttttcga cctcattcta ttagactctc gtttggattg 4440
caactggtct attttcctct tttgtttgat agaaaatcat aaaaggattt gcagactacg 4500
ggcctaaaga actaaaaaat ctatctgttt cttttcattc tctgtatttt ttatagtttc 4560
tgttgcatgg gcataaagtt gcaagcttga tatc 4594
<210> 145
<211> 5043
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 145
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggtac 420
cgggcccccc ctcgaggtcg acggtatcga tacggcatca gagcagattg tactgagagt 480
gcaccataat cggcattttc ttttgcgttt ttatttgtta actgttaatt gtccttgttc 540
aaggatgctg tctttgacaa cagatgtttt cttgcctttg atgttcagca ggaagctagg 600
cgcaaacgtt gattgtttgt ctgcgtagaa tcctctgttt gtcatatagc ttgtaatcac 660
gacattgttt cctttcgctt gaggtacagc gaagtgtgag taagtaaagg ttacatcgtt 720
aggatcaaga tccattttta acacaaggcc agttttgttc agcggcttgt atgggccagt 780
taaagaatta gaaacataac caagcatgta aatatcgtta gacgtaatgc cgtcaatcgt 840
catttttgat ccgcgggagt cagtgaacag ataccatttg ccgttcattt taaagacgtt 900
cgcgcgttca atttcatctg ttactgtgtt agatgcaatc agcggtttca tcactttttt 960
cagtgtgtaa tcatcgttta gctcaatcat accgagagcg ccgtttgcta actcagccgt 1020
gcgtttttta tcgctttgca gaagtttttg actttcttga cggaagaatg atgtgctttt 1080
gccatagtat gctttgttaa ataaagattc ttcgccttgg tagccatctt cagttccagt 1140
gtttgcttca aatactaagt atttgtggcc tttatcttct acgtagtgag gatctctcag 1200
cgtatggttg tcgcctgagc tgtagttgcc ttcatcgatg aactgctgta cattttgata 1260
cgtttttccg tcaccgtcaa agattgattt ataatcctct acaccgttga tgttcaaaga 1320
gctgtctgat gctgatacgt taacttgtgc agttgtcagt gtttgtttgc cgtaatgttt 1380
accggagaaa tcagtgtaga ataaacggat ttttccgtca gatgtaaatg tggctgaacc 1440
tgaccattct tgtgtttggt cttttaggat agaatcattt gcatcgaatt tgtcgctgtc 1500
tttaaagacg cggccagcgt ttttccagct gtcaatagaa gtttcgccga ctttttgata 1560
gaacatgtaa atcgatgtgt catccgcatt tttaggatct ccggctaatg caaagacgat 1620
gtggtagccg tgatagtttg cgacagtgcc gtcagcgttt tgtaatggcc agctgtccca 1680
aacgtccagg ccttttgcag aagagatatt tttaattgtg gacgaatcga actcaggaac 1740
ttgatatttt tcattttttt gctgttcagg gatttgcagc atatcatggc gtgtaatatg 1800
ggaaatgccg tatgtttcct tatatggctt ttggttcgtt tctttcgcaa acgcttgagt 1860
tgcgcctcct gccagcagtg cggtagtaaa ggttaatact gttgcttgtt ttgcaaactt 1920
tttgatgttc atcgttcatg tctccttttt tatgtactgt gttagcggtc tgcttcttcc 1980
agccctcctg tttgaagatg gcaagttagt tacgcacaat aaaaaaagac ctaaaatatg 2040
taaggggtga cgccaaagta tacactttgc cctttacaca ttttaggtct tgcctgcttt 2100
atcagtaaca aacccgcgcg atttactttt cgacctcatt ctattagact ctcgtttgga 2160
ttgcaactgg tctattttcc tcttttgttt gatagaaaat cataaaagga tttgcagact 2220
acgggcctaa agaactaaaa aatctatctg tttcttttca ttctctgtat tttttatagt 2280
ttctgttgca tgggcataaa gttgcaagct tgatatcttg acagctagct cagtcctagg 2340
tataatacta gtgaacgcgg cccggaacat cgaacgccac gcagtgctga tcgatcgaaa 2400
cgtcgcctgc gataggcggg agacgctaaa cgcccgtgga gcatccataa gaccaaccac 2460
ctctcggggc ggtaggcacg acgcatcgaa gcgggaaggc tccggcgctc ggcctgagtc 2520
acctcagcag agtgatctgc tgacgctccc aaccttgaat aacgaaacgg caacgcctcc 2580
atagcggtgc aggtcaataa gggtcggccc cacgcgtgta gggagcgatc gtgtttgtta 2640
ccaactgcct gggccggcat ggtcccagcc tcctcgctgg cgccggctgg gcaacatgct 2700
tcggcatggc gaatgggacc caattattga acaccctaac gggtgttttt ttgtttctgg 2760
tctaccgata tcgaattcct gcagcccggg ggatccacta gttctagagc ggccgccacc 2820
gcggtggagc tcatcccaat ggcgcgccga gcttggctcg agcatggtca tagctgtttc 2880
ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 2940
gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 3000
ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 3060
ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 3120
cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 3180
cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 3240
accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 3300
acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 3360
cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 3420
acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 3480
atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 3540
agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 3600
acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 3660
gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 3720
gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 3780
gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 3840
gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 3900
acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 3960
tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 4020
ctgacagtta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat 4080
tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc 4140
agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa 4200
tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag 4260
tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa 4320
caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc 4380
gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag 4440
gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat 4500
caggatattc ttctaatacc tggaatgctg ttttcccagg gatcgcagtg gtgagtaacc 4560
atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca 4620
gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt 4680
tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt 4740
gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta 4800
atcgcggcct agagcaagac gtttcccgtt gaatatggct catactcttc ctttttcaat 4860
attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 4920
agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct 4980
aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 5040
gtc 5043
<210> 146
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 146
tgtttgttac caactgcctg 20
<210> 147
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 147
atcgtgtttg ttaccaactg cctg 24
<210> 148
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 148
ggcccaggca gttggtaaca aaca 24
<210> 149
<211> 90
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 149
tgcgggatct caaaatccag cgatgagccg cccaccagca ccacagcgtt atcgatcggc 60
accagttcgc cctccttgat gtacaccact 90
<210> 150
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 150
tcatcattgg catcctgaaa 20
<210> 151
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 151
tggtcagcct gctgattaaa 20
<210> 152
<211> 7178
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 152
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 60
cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 120
ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 180
gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 240
acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 300
ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 360
ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 420
acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 480
tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 540
tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat 600
tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 660
actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 720
gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 780
aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc 840
agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac 900
cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac 960
aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat 1020
tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag 1080
tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca 1140
taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac 1200
ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg 1260
tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca 1320
tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac 1380
cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa 1440
cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 1500
gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1560
gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1620
agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 1680
aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1740
agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1800
cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1860
accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1920
aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1980
ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 2040
cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 2100
gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 2160
tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 2220
agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg 2280
tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta 2340
caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 2400
ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 2460
gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 2520
gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc 2580
gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag 2640
aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt 2700
ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa 2760
acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg 2820
ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg 2880
tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc 2940
tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta 3000
cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca 3060
gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc 3120
ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc 3180
catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa 3240
ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc 3300
gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac 3360
gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca 3420
ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta 3480
atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 3540
cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 3600
tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca 3660
ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa 3720
aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt 3780
atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg 3840
cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca 3900
gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta 3960
tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg 4020
agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat 4080
gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct 4140
ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg 4200
catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat 4260
tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc 4320
tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca 4380
gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg 4440
ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt 4500
tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg 4560
catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct 4620
cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga 4680
tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg 4740
ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc 4800
ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg 4860
cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg 4920
gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga 4980
aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa 5040
ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac 5100
agcagcggcc tggtgccgcg cggcagcatg tcggactcag aagtcaatca agaagctaag 5160
ccagaggtca agccagaagt caagcctgag actcacatca atttaaaggt gtccgatgga 5220
tcttcagaga tcttcttcaa gatcaaaaag accactcctt taagaaggct gatggaagcg 5280
ttcgctaaaa gacagggtaa ggaaatggac tccttaagat tcttgtacga cggtattaga 5340
attcaagctg atcagacccc tgaagatttg gacatggagg ataacgatat tattgaggct 5400
cacagagaac agattggtgg tcatagtagt cttgaagtcc tctttcaggg acccatggtg 5460
cagacggaga tcctgaaggc gtttcggttt gcgttggatc cgacgtctgt gcaggttgct 5520
gccctttccc ggcatgctgg tgcggccagg tgggcgttca accatgctct cgcggccaag 5580
gtcggcgcgc atgagcggtg gcgggcggag gtagccaagc ttgtcgggga cggtgttcct 5640
gaggagcagg ctcgtaggca ggtgcgcgtc ccggttccga tgaagccggc gatccagaag 5700
gcgttgaacg cggtcaaggg tgactctcgc aagggcttgg atggtgcgtg cccgtggtgg 5760
catgaggtca acacttacgc gttccagtcc gctttcatcg acgcggacca ggcgtggaag 5820
aactggttgg actcgttgtc ggggaagcgg gtcggccgcc gggtggggta cccgcggttc 5880
aagaagaagg ggcgggcccg cgactcgttt cgcttgcacc acgacgtgaa gaagccaagc 5940
atccggctgg ccgggtaccg gcggttgcgg ctgccgagga tcggggaagt ccggcttcat 6000
gactcgggga agcgtctggc acggctgatc gaccgaggtg acgcggtggt gcagtcggtg 6060
acggtgtcgc ggggtggcca tcgctggtat gcgtctgtgc tgtgcaaggt caccgtgcag 6120
gtaccggatc ggccgtcacg gcgccagcgg gaacgaggcg ctgttggggt ggaccttggc 6180
gttaaggtcc tcgcggcttt gtccaagcca ctggtggtcg acgacccgtc gagtgccctg 6240
gtgcggaatc cgcagcatct gcggcaggcc gagcggcgtc tactgaaggc tcagcgggcg 6300
ttggcgcgta cacaaaaagg ttcagcccga cgcgagaagg ccaagcggcg agttggtcgc 6360
gcgcatcacg aggttgctgt gcgccggcat gctgcgctcc atcagatcac caagcgcctg 6420
accaccggct tcgcggtcgt cgcgcttgag gatctgaacg tggccggtat gacaaggtca 6480
gcgcggggaa cagtggcagc gccgggaaag aacgtgcggc agaaggcagg cctgaaccgc 6540
gtcatcctgg actccgctcc agccgagttg cgtcgccagg tgaactacaa ggccacctgg 6600
tatggatctg agctggcggt cgcagaccgc tggttcccct cgagtaagac ctgttcgggt 6660
tgcggatggc aaaacccaca cctgaagctg tccgacaggg tgtttcgttg cacagactgc 6720
gggctggtga tggaccggga catgaacgcg gcccggaaca tcgaacgcca cgcagtgctg 6780
atcgatcgaa acgtcgcctg cgataggcgg gagacgctaa acgcccgtgg agcatccata 6840
agaccaacca cctctcgggg cggtaggcac gacgcatcga agcgggaagg ctccggcgct 6900
cggcctgagt cacctcagca gagtgatctg ctgacgctcc caaccttgaa taacgaaacg 6960
gcaacgcctc cataagctta agaattcgag ctccgtcgac aagcttgcgg ccgcactcga 7020
gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt 7080
ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt 7140
gaggggtttt ttgctgaaag gaggaactat atccggat 7178
<210> 153
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 153
tcttgaagtc ctctttcagg gacccatggt gcagacggag atcctg 46
<210> 154
<211> 47
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 154
gtcgacggag ctcgaattct taagcttatg gaggcgttgc cgtttcg 47
<210> 155
<211> 5657
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 155
gcttaagaat tcgagctccg tcgacaagct tgcggccgca ctcgagcacc accaccacca 60
ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc 120
tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct 180
gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc 240
gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 300
gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 360
ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 420
aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 480
cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 540
ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 600
tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg 660
tttacaattt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 720
ttctaaatac attcaaatat gtatccgctc atgaattaat tcttagaaaa actcatcgag 780
catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag 840
ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg 900
gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc 960
aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg 1020
caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc 1080
aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa 1140
tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa 1200
cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa 1260
tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa 1320
atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc 1380
tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg 1440
cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt 1500
atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctagagc aagacgtttc 1560
ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag acagttttat 1620
tgttcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1680
aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1740
caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1800
ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 1860
cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1920
tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1980
gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 2040
ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 2100
gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 2160
caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 2220
ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 2280
tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 2340
ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 2400
agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 2460
aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc 2520
gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac 2580
actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct 2640
gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc 2700
tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg 2760
cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg 2820
tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg 2880
ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc 2940
atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat 3000
gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg 3060
gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt 3120
ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct 3180
gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct 3240
caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca 3300
ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg 3360
atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt 3420
ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca 3480
agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag 3540
agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg 3600
acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc 3660
atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca 3720
ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 3780
gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac 3840
gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac 3900
gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca 3960
tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc 4020
ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc 4080
agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact 4140
ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca 4200
gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg 4260
ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa 4320
aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt 4380
gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc 4440
actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg 4500
ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc 4560
gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga 4620
ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc 4680
cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga 4740
aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac 4800
attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt 4860
gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga 4920
agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca 4980
aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac 5040
aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat 5100
aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga 5160
ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga 5220
taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat accatgggca 5280
gcagccatca tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc atgtcggact 5340
cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct gagactcaca 5400
tcaatttaaa ggtgtccgat ggatcttcag agatcttctt caagatcaaa aagaccactc 5460
ctttaagaag gctgatggaa gcgttcgcta aaagacaggg taaggaaatg gactccttaa 5520
gattcttgta cgacggtatt agaattcaag ctgatcagac ccctgaagat ttggacatgg 5580
aggataacga tattattgag gctcacagag aacagattgg tggtcatagt agtcttgaag 5640
tcctctttca gggaccc 5657
<210> 156
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 156
ccgcatatat cgcaaaacaa 20
<210> 157
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 157
ctggctgtga acaaagtgga 20
<210> 158
<211> 254
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 158
aaacgucgcc ugcgauaggc gggagacgcu aaacgcccgu ggagcaucca uaagaccaac 60
caccucucgg ggcgguaggc acgacgcauc gaagcgggaa ggcuccggcg cucggccuga 120
gucaccucag cagagugauc ugcugacgcu cccaaccuug aauaacgaaa cggcaacgcc 180
uccauagcgg ugcaggucaa uaagggucgg ccccacgcgu guagggagcg aucgcgguuc 240
aggugaaagu gaaa 254
<210> 159
<211> 274
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 159
taatacgact cactataggg aaacgtcgcc tgcgataggc gggagacgct aaacgcccgt 60
ggagcatcca taagaccaac cacctctcgg ggcggtaggc acgacgcatc gaagcgggaa 120
ggctccggcg ctcggcctga gtcacctcag cagagtgatc tgctgacgct cccaaccttg 180
aataacgaaa cggcaacgcc tccatagcgg tgcaggtcaa taagggtcgg ccccacgcgt 240
gtagggagcg atcgcggttc aggtgaaagt gaaa 274
<210> 160
<211> 205
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 160
aaacgucgcc ugcgauaggc gggagacgcu aaacgcccgu ggagcaucca uaagaccaac 60
caccucucgg ggcgguaggc acgacgcauc gaagcgggaa ggcuccggcg cucggccuga 120
gucaccucag cagagugauc ugcugacgcu cccaaccuug aauaacgaaa cggcaacgcc 180
uccauagcgg ugcaggucaa uaagg 205
<210> 161
<211> 225
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 161
taatacgact cactataggg aaacgtcgcc tgcgataggc gggagacgct aaacgcccgt 60
ggagcatcca taagaccaac cacctctcgg ggcggtaggc acgacgcatc gaagcgggaa 120
ggctccggcg ctcggcctga gtcacctcag cagagtgatc tgctgacgct cccaaccttg 180
aataacgaaa cggcaacgcc tccatagcgg tgcaggtcaa taagg 225
<210> 162
<211> 49
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 162
gucggcccca cgcguguagg gagcgaucgc gguucaggug aaagugaaa 49
<210> 163
<211> 69
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 163
taatacgact cactataggg gtcggcccca cgcgtgtagg gagcgatcgc ggttcaggtg 60
aaagtgaaa 69
<210> 164
<211> 58
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 164
ggctgtgaga aagcggttca ggtgaaagtg aaaacactgc ccgacgccca gttcgaag 58
<210> 165
<211> 58
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 165
cttcgaactg ggcgtcgggc agtgttttca ctttcacctg aaccgctttc tcacagcc 58
<210> 166
<211> 7204
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 166
ggtaccgatt agtgaacgga tctcgacggt atcgatcacg agactagcct cgagcggccg 60
cccccttcac cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg 120
ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata 180
cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa 240
tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct 300
tgtggaaagg acgaaacacc gaacgcggcc cggaacatcg aacgccacgc agtgctgatc 360
gatcgaaacg tcgcctgcga taggcgggag acgctaaacg cccgtggagc atccataaga 420
ccaaccacct ctcggggcgg taggcacgac gcatcgaagc gggaaggctc cggcgctcgg 480
cctgagtcac ctcagcagag tgatctgctg acgctcccaa ccttgaataa cgaaacggca 540
acgcctccat agcggtgcag gtcaataagg gtcggcccca cgcgtgtagg gagcgatcgc 600
gagacccgag agagggtcgg tctccttttt ttgaattctc gacctcgaga caaatggcag 660
tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa 720
tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa 780
ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggccg cggctcgagg 840
gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct 900
gggcgtggtt ccgggaaacg cagcggcgcc gaccctggga ctcgcacatt cttcacgtcc 960
gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct 1020
gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg 1080
gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg 1140
cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc 1200
ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg 1260
cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc 1320
gttgaccgaa tcaccgacct ctctccccag ggggatccac cggagcttac catgaccgag 1380
tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 1440
gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 1500
gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 1560
gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 1620
gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 1680
gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 1740
ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 1800
gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggaaacctcc 1860
gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 1920
gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc 1980
cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatcggt acctttaaga 2040
ccaatgactt acaaggcagc tgtagatctt agccactttc tagagtcggg gcggccggcc 2100
gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca 2160
gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat 2220
aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg 2280
ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa 2340
ggatccgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc 2400
ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac 2460
aggtgccggc agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 2520
ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 2580
gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 2640
gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 2700
cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 2760
ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 2820
tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg 2880
gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 2940
tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 3000
ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 3060
ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct 3120
ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 3180
accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 3240
tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 3300
cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 3360
taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 3420
caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 3480
gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 3540
gctgcaatga taccgcggga cccacgctca ccggctccag atttatcagc aataaaccag 3600
ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 3660
attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 3720
gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 3780
tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 3840
agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 3900
gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 3960
actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 4020
tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 4080
attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 4140
tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 4200
tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 4260
aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 4320
tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 4380
cgcacatttc cccgaaaagt gccacctgca ggaaacagct atgaccatga ttacgccaag 4440
ctctagctag aggtcgacgg tatcgagccc cagctggttc tttccgcctc agaagccata 4500
gagcccaccg catccccagc atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc 4560
tgccccaccc caccccccag aatagaatga cacctactca gacaatgcga tgcaatttcc 4620
tcattttatt aggaaaggac agtgggagtg gcaccttcca gggtcaagga aggcacgggg 4680
gaggggcaaa caacagatgg ctggcaacta gaaggcacag tcgaggctga tcagcgggtt 4740
taaactcaat ggtgatggtg atgatgaccg gttagacttt cctcttcttc ttgggagaac 4800
caccagaggg gggtgtggcg gtttcgttgt tcagtgtggg gagtgtgagc agatcagact 4860
gctgggggga ctctggccgg gccccgctcc cctccctctt gctggcgtcg tgccgccccc 4920
cccggcttgt ggtgggccgg atgctggccc ccctggcgtt caaagtctcc cgccggtcgc 4980
aggccacgtt cctatcaatc aacacagcgt gcctctcgat gttcctagcg gcgttcatat 5040
ctctatccat gaccaggccg cagtcggtgc acctaaacac cctatcggag agcttcaagt 5100
gggggttctg ccagccgcac ccgctgcagg tcttgctgga ggggaaccac ctgtcggcca 5160
cggccagctc gctcccgtac caggtggcct tgtagttcac ctgcctccgc agctcggcag 5220
gggcgctgtc caggatgacc cggttcagcc cggccttctg ccggacgttc ttccctgggg 5280
cggccactgt ccccctggcg gacctggtca tgccggcgac gttcagatcc tccagggcca 5340
ccacggcgaa ccctgtggtc agccgcttgg tgatctggtg cagggcggcg tgccgccgga 5400
cggccacctc gtgatgggcc ctccccaccc gccgcttggc cttctctctc ctggcgctgc 5460
ccttctgtgt cctggccagg gcccgctggg ccttgagcag ccgcctctca gcctgcctca 5520
ggtgctgggg gtttctcacc agggcgctgc ttgggtcgtc gacgaccagg ggcttggaca 5580
gggcggccag caccttcacc cccaggtcca ctcccacggc gcccctctcc cgctgccgcc 5640
tgctgggccg atcgggcacc tgcactgtca ccttgcagag cacggaggcg taccacctgt 5700
gtccgcccct actcacagtc acggactgca ccacggcgtc gcctctgtca atgagcctgg 5760
cgagccgctt gccgctgtcg tgcagcctca cctcgccaat cctaggcagc ctcagccttc 5820
tgtagccagc cagcctgatg ctgggcttct tcacatcgtg gtgcaatcta aagctatccc 5880
gagcccggcc cttcttctta aaccgggggt agcccaccct cctccccact ctcttgccgc 5940
tcaggctgtc gagccagttc ttccatgcct ggtcggcgtc aataaaggcg ctctggaagg 6000
cgtaggtgtt cacctcgtgc caccatgggc aggccccatc caggcccttc cgggaatccc 6060
ccttcacggc gttcagggcc ttctggatgg ctggcttcat aggcacgggg acccggacct 6120
gccgccgggc ctgctcctcg ggcacgccat cgcccacgag cttggccacc tcggcccgcc 6180
accgctcgtg ggcccccacc ttggcggcga gggcgtggtt gaaggcccac ctggcggccc 6240
cggcgtgccg gctcagggcg gccacctgga cggaagtagg atcgagggcg aaccgaaagg 6300
ccttcaggat ctctgtctgc accatgacct tcctcttctt cttaggggcc atggtggcgg 6360
ctctccctat agtgagtcgt attagcggcc gcggatctct agcggatctg acggttcact 6420
aaaccagctc tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat 6480
ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc 6540
ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc 6600
ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg 6660
tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat 6720
ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc 6780
aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc 6840
gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag 6900
ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact 6960
aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtca acgcgtatat 7020
ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc taagcagatt cttcatgcaa 7080
ttgtcggtca agaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa 7140
acaaactagc aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctctatc 7200
gata 7204
<210> 167
<211> 2491
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 167
aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt 60
taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc gaccgatgcc 120
cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc 180
cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt 240
ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 300
ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 360
tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 420
tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 480
gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 540
ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 600
tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 660
agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 720
atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 780
acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 840
actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 900
tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 960
tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 1020
tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 1080
tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 1140
caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 1200
cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 1260
agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcggg 1320
acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 1380
gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 1440
ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 1500
tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 1560
ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 1620
tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 1680
attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 1740
agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 1800
ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 1860
ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 1920
cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 1980
gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 2040
tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 2100
tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 2160
tgccacctgc aggaaacagc tatgaccatg attacgccaa gctctagcta gaggtcgacg 2220
gtatcgagcc ccagctggtt ctttccgcct cagaagccat agagcccacc gcatccccag 2280
catgcctgct attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca 2340
gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga 2400
cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg 2460
gctggcaact agaaggcaca gtcgaggctg a 2491
<210> 168
<211> 1566
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 168
ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat 60
agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg 120
ggtttattac agggacagca gagatccact ttggccgcgg ctcgaggggg ttggggttgc 180
gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg 240
ggaaacgcag cggcgccgac cctgggactc gcacattctt cacgtccgtt cgcagcgtca 300
cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa 360
gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc 420
tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat 480
gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg 540
cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt 600
ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca 660
ccgacctctc tccccagggg gatccaccgg agcttaccat gaccgagtac aagcccacgg 720
tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg 780
ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg 840
agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg 900
acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt 960
tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac 1020
agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg 1080
tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag 1140
tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga aacctccgcg ccccgcaacc 1200
tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac 1260
cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc 1320
gcccgaccga aaggagcgca cgaccccatg catcggtacc tttaagacca atgacttaca 1380
aggcagctgt agatcttagc cactttctag agtcggggcg gccggccgct tcgagcagac 1440
atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc 1500
tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa 1560
caagtt 1566
<210> 169
<211> 2474
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 169
tcagcgggtt taaactcaat ggtgatggtg atgatgaccg gttagacttt cctcttcttc 60
ttgggagaac caccagaggg gggtgtggcg gtttcgttgt tcagtgtggg gagtgtgagc 120
agatcagact gctgggggga ctctggccgg gccccgctcc cctccctctt gctggcgtcg 180
tgccgccccc cccggcttgt ggtgggccgg atgctggccc ccctggcgtt caaagtctcc 240
cgccggtcgc aggccacgtt cctatcaatc aacacagcgt gcctctcgat gttcctagcg 300
gcgttcatat ctctatccat gaccaggccg cagtcggtgc acctaaacac cctatcggag 360
agcttcaagt gggggttctg ccagccgcac ccgctgcagg tcttgctgga ggggaaccac 420
ctgtcggcca cggccagctc gctcccgtac caggtggcct tgtagttcac ctgcctccgc 480
agctcggcag gggcgctgtc caggatgacc cggttcagcc cggccttctg ccggacgttc 540
ttccctgggg cggccactgt ccccctggcg gacctggtca tgccggcgac gttcagatcc 600
tccagggcca ccacggcgaa ccctgtggtc agccgcttgg tgatctggtg cagggcggcg 660
tgccgccgga cggccacctc gtgatgggcc ctccccaccc gccgcttggc cttctctctc 720
ctggcgctgc ccttctgtgt cctggccagg gcccgctggg ccttgagcag ccgcctctca 780
gcctgcctca ggtgctgggg gtttctcacc agggcgctgc ttgggtcgtc gacgaccagg 840
ggcttggaca gggcggccag caccttcacc cccaggtcca ctcccacggc gcccctctcc 900
cgctgccgcc tgctgggccg atcgggcacc tgcactgtca ccttgcagag cacggaggcg 960
taccacctgt gtccgcccct actcacagtc acggactgca ccacggcgtc gcctctgtca 1020
atgagcctgg cgagccgctt gccgctgtcg tgcagcctca cctcgccaat cctaggcagc 1080
ctcagccttc tgtagccagc cagcctgatg ctgggcttct tcacatcgtg gtgcaatcta 1140
aagctatccc gagcccggcc cttcttctta aaccgggggt agcccaccct cctccccact 1200
ctcttgccgc tcaggctgtc gagccagttc ttccatgcct ggtcggcgtc aataaaggcg 1260
ctctggaagg cgtaggtgtt cacctcgtgc caccatgggc aggccccatc caggcccttc 1320
cgggaatccc ccttcacggc gttcagggcc ttctggatgg ctggcttcat aggcacgggg 1380
acccggacct gccgccgggc ctgctcctcg ggcacgccat cgcccacgag cttggccacc 1440
tcggcccgcc accgctcgtg ggcccccacc ttggcggcga gggcgtggtt gaaggcccac 1500
ctggcggccc cggcgtgccg gctcagggcg gccacctgga cggaagtagg atcgagggcg 1560
aaccgaaagg ccttcaggat ctctgtctgc accatgacct tcctcttctt cttaggggcc 1620
atggtggcgg ctctccctat agtgagtcgt attagcggcc gcggatctct agcggatctg 1680
acggttcact aaaccagctc tgcttatata gacctcccac cgtacacgcc taccgcccat 1740
ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca 1800
aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg 1860
ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa 1920
tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca 1980
ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg 2040
atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt 2100
ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt 2160
gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg 2220
gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtca 2280
acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc taagcagatt 2340
cttcatgcaa ttgtcggtca agaatcgata gtactaacat acgctctcca tcaaaacaaa 2400
acgaaacaaa acaaactagc aaaataggct gtccccagtg caagtgcagg tgccagaaca 2460
tttctctatc gata 2474
<210> 170
<211> 673
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 170
ggtaccgatt agtgaacgga tctcgacggt atcgatcacg agactagcct cgagcggccg 60
cccccttcac cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg 120
ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata 180
cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa 240
tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct 300
tgtggaaagg acgaaacacc gaacgcggcc cggaacatcg aacgccacgc agtgctgatc 360
gatcgaaacg tcgcctgcga taggcgggag acgctaaacg cccgtggagc atccataaga 420
ccaaccacct ctcggggcgg taggcacgac gcatcgaagc gggaaggctc cggcgctcgg 480
cctgagtcac ctcagcagag tgatctgctg acgctcccaa ccttgaataa cgaaacggca 540
acgcctccat agcggtgcag gtcaataagg gtcggcccca cgcgtgtagg gagcgatcgc 600
gagacccgag agagggtcgg tctccttttt ttgaattctc gacctcgaga caaatggcag 660
tattcatcca caa 673
<210> 171
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 171
atcgagtgac agtatcctct gtat 24
<210> 172
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 172
aaaaatacag aggatactgt cact 24
<210> 173
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 173
atcgcagctc atttacagac ggga 24
<210> 174
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 174
aaaatcccgt ctgtaaatga gctg 24
<210> 175
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 175
atcggagctc tacaccacac ccaa 24
<210> 176
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 176
aaaattgggt gtggtgtaga gctc 24
<210> 177
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 177
atcgttagcc tggcatggtg gtgc 24
<210> 178
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 178
aaaagcacca ccatgccagg ctaa 24
<210> 179
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 179
atcgttaatt tctaattccg gaat 24
<210> 180
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 180
aaaaattccg gaattagaaa ttaa 24
<210> 181
<211> 7198
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 181
ggtaccgatt agtgaacgga tctcgacggt atcgatcacg agactagcct cgagcggccg 60
cccccttcac cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg 120
ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata 180
cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa 240
tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct 300
tgtggaaagg acgaaacacc gaacgcggcc cggaacatcg aacgccacgc agtgctgatc 360
gatcgaaacg tcgcctgcga taggcgggag acgctaaacg cccgtggagc atccataaga 420
ccaaccacct ctcggggcgg taggcacgac gcatcgaagc gggaaggctc cggcgctcgg 480
cctgagtcac ctcagcagag tgatctgctg acgctcccaa ccttgaataa cgaaacggca 540
acgcctccat agcggtgcag gtcaataagg gtcggcccca cgcgtgtagg gagcgatcga 600
gtgacagtat cctctgtatt ttttttgaat tctcgacctc gagacaaatg gcagtattca 660
tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag 720
acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa 780
attttcgggt ttattacagg gacagcagag atccactttg gccgcggctc gagggggttg 840
gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt 900
ggttccggga aacgcagcgg cgccgaccct gggactcgca cattcttcac gtccgttcgc 960
agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg 1020
cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc 1080
gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga 1140
ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg 1200
gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg 1260
cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac 1320
cgaatcaccg acctctctcc ccagggggat ccaccggagc ttaccatgac cgagtacaag 1380
cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 1440
gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 1500
gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 1560
gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 1620
gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 1680
cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 1740
gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 1800
cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggaaac ctccgcgccc 1860
cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 1920
gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac 1980
ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cggtaccttt aagaccaatg 2040
acttacaagg cagctgtaga tcttagccac tttctagagt cggggcggcc ggccgcttcg 2100
agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa 2160
aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg 2220
caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt 2280
gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc 2340
gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca 2400
tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc 2460
cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 2520
cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 2580
gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 2640
ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 2700
agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 2760
tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 2820
ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag 2880
gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 2940
ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 3000
gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 3060
aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 3120
aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 3180
ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 3240
gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 3300
gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 3360
tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 3420
ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 3480
ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 3540
atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 3600
ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 3660
tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 3720
attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 3780
tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 3840
ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 3900
gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 3960
gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 4020
gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 4080
aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 4140
taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 4200
tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 4260
tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 4320
atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 4380
tttccccgaa aagtgccacc tgcaggaaac agctatgacc atgattacgc caagctctag 4440
ctagaggtcg acggtatcga gccccagctg gttctttccg cctcagaagc catagagccc 4500
accgcatccc cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc 4560
accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt 4620
tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg 4680
caaacaacag atggctggca actagaaggc acagtcgagg ctgatcagcg ggtttaaact 4740
caatggtgat ggtgatgatg accggttaga ctttcctctt cttcttggga gaaccaccag 4800
aggggggtgt ggcggtttcg ttgttcagtg tggggagtgt gagcagatca gactgctggg 4860
gggactctgg ccgggccccg ctcccctccc tcttgctggc gtcgtgccgc cccccccggc 4920
ttgtggtggg ccggatgctg gcccccctgg cgttcaaagt ctcccgccgg tcgcaggcca 4980
cgttcctatc aatcaacaca gcgtgcctct cgatgttcct agcggcgttc atatctctat 5040
ccatgaccag gccgcagtcg gtgcacctaa acaccctatc ggagagcttc aagtgggggt 5100
tctgccagcc gcacccgctg caggtcttgc tggaggggaa ccacctgtcg gccacggcca 5160
gctcgctccc gtaccaggtg gccttgtagt tcacctgcct ccgcagctcg gcaggggcgc 5220
tgtccaggat gacccggttc agcccggcct tctgccggac gttcttccct ggggcggcca 5280
ctgtccccct ggcggacctg gtcatgccgg cgacgttcag atcctccagg gccaccacgg 5340
cgaaccctgt ggtcagccgc ttggtgatct ggtgcagggc ggcgtgccgc cggacggcca 5400
cctcgtgatg ggccctcccc acccgccgct tggccttctc tctcctggcg ctgcccttct 5460
gtgtcctggc cagggcccgc tgggccttga gcagccgcct ctcagcctgc ctcaggtgct 5520
gggggtttct caccagggcg ctgcttgggt cgtcgacgac caggggcttg gacagggcgg 5580
ccagcacctt cacccccagg tccactccca cggcgcccct ctcccgctgc cgcctgctgg 5640
gccgatcggg cacctgcact gtcaccttgc agagcacgga ggcgtaccac ctgtgtccgc 5700
ccctactcac agtcacggac tgcaccacgg cgtcgcctct gtcaatgagc ctggcgagcc 5760
gcttgccgct gtcgtgcagc ctcacctcgc caatcctagg cagcctcagc cttctgtagc 5820
cagccagcct gatgctgggc ttcttcacat cgtggtgcaa tctaaagcta tcccgagccc 5880
ggcccttctt cttaaaccgg gggtagccca ccctcctccc cactctcttg ccgctcaggc 5940
tgtcgagcca gttcttccat gcctggtcgg cgtcaataaa ggcgctctgg aaggcgtagg 6000
tgttcacctc gtgccaccat gggcaggccc catccaggcc cttccgggaa tcccccttca 6060
cggcgttcag ggccttctgg atggctggct tcataggcac ggggacccgg acctgccgcc 6120
gggcctgctc ctcgggcacg ccatcgccca cgagcttggc cacctcggcc cgccaccgct 6180
cgtgggcccc caccttggcg gcgagggcgt ggttgaaggc ccacctggcg gccccggcgt 6240
gccggctcag ggcggccacc tggacggaag taggatcgag ggcgaaccga aaggccttca 6300
ggatctctgt ctgcaccatg accttcctct tcttcttagg ggccatggtg gcggctctcc 6360
ctatagtgag tcgtattagc ggccgcggat ctctagcgga tctgacggtt cactaaacca 6420
gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg 6480
gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg 6540
acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg 6600
atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc 6660
caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg 6720
tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg 6780
gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact 6840
atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc 6900
gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac 6960
cccgtaattg attactatta ataactagtc aataatcaat gtcaacgcgt atatctggcc 7020
cgtacatcgc gaagcagcgc aaaacgccta accctaagca gattcttcat gcaattgtcg 7080
gtcaagaatc gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac 7140
tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc tatcgata 7198
<210> 182
<211> 7198
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 182
ggtaccgatt agtgaacgga tctcgacggt atcgatcacg agactagcct cgagcggccg 60
cccccttcac cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg 120
ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata 180
cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa 240
tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct 300
tgtggaaagg acgaaacacc gaacgcggcc cggaacatcg aacgccacgc agtgctgatc 360
gatcgaaacg tcgcctgcga taggcgggag acgctaaacg cccgtggagc atccataaga 420
ccaaccacct ctcggggcgg taggcacgac gcatcgaagc gggaaggctc cggcgctcgg 480
cctgagtcac ctcagcagag tgatctgctg acgctcccaa ccttgaataa cgaaacggca 540
acgcctccat agcggtgcag gtcaataagg gtcggcccca cgcgtgtagg gagcgatcgc 600
agctcattta cagacgggat ttttttgaat tctcgacctc gagacaaatg gcagtattca 660
tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag 720
acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa 780
attttcgggt ttattacagg gacagcagag atccactttg gccgcggctc gagggggttg 840
gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt 900
ggttccggga aacgcagcgg cgccgaccct gggactcgca cattcttcac gtccgttcgc 960
agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg 1020
cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc 1080
gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga 1140
ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg 1200
gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg 1260
cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac 1320
cgaatcaccg acctctctcc ccagggggat ccaccggagc ttaccatgac cgagtacaag 1380
cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 1440
gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 1500
gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 1560
gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 1620
gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 1680
cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 1740
gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 1800
cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggaaac ctccgcgccc 1860
cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 1920
gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac 1980
ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cggtaccttt aagaccaatg 2040
acttacaagg cagctgtaga tcttagccac tttctagagt cggggcggcc ggccgcttcg 2100
agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa 2160
aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg 2220
caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt 2280
gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc 2340
gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca 2400
tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc 2460
cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 2520
cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 2580
gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 2640
ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 2700
agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 2760
tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 2820
ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag 2880
gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 2940
ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 3000
gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 3060
aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 3120
aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 3180
ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 3240
gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 3300
gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 3360
tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 3420
ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 3480
ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 3540
atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 3600
ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 3660
tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 3720
attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 3780
tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 3840
ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 3900
gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 3960
gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 4020
gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 4080
aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 4140
taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 4200
tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 4260
tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 4320
atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 4380
tttccccgaa aagtgccacc tgcaggaaac agctatgacc atgattacgc caagctctag 4440
ctagaggtcg acggtatcga gccccagctg gttctttccg cctcagaagc catagagccc 4500
accgcatccc cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc 4560
accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt 4620
tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg 4680
caaacaacag atggctggca actagaaggc acagtcgagg ctgatcagcg ggtttaaact 4740
caatggtgat ggtgatgatg accggttaga ctttcctctt cttcttggga gaaccaccag 4800
aggggggtgt ggcggtttcg ttgttcagtg tggggagtgt gagcagatca gactgctggg 4860
gggactctgg ccgggccccg ctcccctccc tcttgctggc gtcgtgccgc cccccccggc 4920
ttgtggtggg ccggatgctg gcccccctgg cgttcaaagt ctcccgccgg tcgcaggcca 4980
cgttcctatc aatcaacaca gcgtgcctct cgatgttcct agcggcgttc atatctctat 5040
ccatgaccag gccgcagtcg gtgcacctaa acaccctatc ggagagcttc aagtgggggt 5100
tctgccagcc gcacccgctg caggtcttgc tggaggggaa ccacctgtcg gccacggcca 5160
gctcgctccc gtaccaggtg gccttgtagt tcacctgcct ccgcagctcg gcaggggcgc 5220
tgtccaggat gacccggttc agcccggcct tctgccggac gttcttccct ggggcggcca 5280
ctgtccccct ggcggacctg gtcatgccgg cgacgttcag atcctccagg gccaccacgg 5340
cgaaccctgt ggtcagccgc ttggtgatct ggtgcagggc ggcgtgccgc cggacggcca 5400
cctcgtgatg ggccctcccc acccgccgct tggccttctc tctcctggcg ctgcccttct 5460
gtgtcctggc cagggcccgc tgggccttga gcagccgcct ctcagcctgc ctcaggtgct 5520
gggggtttct caccagggcg ctgcttgggt cgtcgacgac caggggcttg gacagggcgg 5580
ccagcacctt cacccccagg tccactccca cggcgcccct ctcccgctgc cgcctgctgg 5640
gccgatcggg cacctgcact gtcaccttgc agagcacgga ggcgtaccac ctgtgtccgc 5700
ccctactcac agtcacggac tgcaccacgg cgtcgcctct gtcaatgagc ctggcgagcc 5760
gcttgccgct gtcgtgcagc ctcacctcgc caatcctagg cagcctcagc cttctgtagc 5820
cagccagcct gatgctgggc ttcttcacat cgtggtgcaa tctaaagcta tcccgagccc 5880
ggcccttctt cttaaaccgg gggtagccca ccctcctccc cactctcttg ccgctcaggc 5940
tgtcgagcca gttcttccat gcctggtcgg cgtcaataaa ggcgctctgg aaggcgtagg 6000
tgttcacctc gtgccaccat gggcaggccc catccaggcc cttccgggaa tcccccttca 6060
cggcgttcag ggccttctgg atggctggct tcataggcac ggggacccgg acctgccgcc 6120
gggcctgctc ctcgggcacg ccatcgccca cgagcttggc cacctcggcc cgccaccgct 6180
cgtgggcccc caccttggcg gcgagggcgt ggttgaaggc ccacctggcg gccccggcgt 6240
gccggctcag ggcggccacc tggacggaag taggatcgag ggcgaaccga aaggccttca 6300
ggatctctgt ctgcaccatg accttcctct tcttcttagg ggccatggtg gcggctctcc 6360
ctatagtgag tcgtattagc ggccgcggat ctctagcgga tctgacggtt cactaaacca 6420
gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg 6480
gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg 6540
acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg 6600
atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc 6660
caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg 6720
tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg 6780
gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact 6840
atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc 6900
gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac 6960
cccgtaattg attactatta ataactagtc aataatcaat gtcaacgcgt atatctggcc 7020
cgtacatcgc gaagcagcgc aaaacgccta accctaagca gattcttcat gcaattgtcg 7080
gtcaagaatc gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac 7140
tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc tatcgata 7198
<210> 183
<211> 7198
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 183
ggtaccgatt agtgaacgga tctcgacggt atcgatcacg agactagcct cgagcggccg 60
cccccttcac cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg 120
ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata 180
cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa 240
tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct 300
tgtggaaagg acgaaacacc gaacgcggcc cggaacatcg aacgccacgc agtgctgatc 360
gatcgaaacg tcgcctgcga taggcgggag acgctaaacg cccgtggagc atccataaga 420
ccaaccacct ctcggggcgg taggcacgac gcatcgaagc gggaaggctc cggcgctcgg 480
cctgagtcac ctcagcagag tgatctgctg acgctcccaa ccttgaataa cgaaacggca 540
acgcctccat agcggtgcag gtcaataagg gtcggcccca cgcgtgtagg gagcgatcgg 600
agctctacac cacacccaat ttttttgaat tctcgacctc gagacaaatg gcagtattca 660
tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag 720
acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa 780
attttcgggt ttattacagg gacagcagag atccactttg gccgcggctc gagggggttg 840
gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt 900
ggttccggga aacgcagcgg cgccgaccct gggactcgca cattcttcac gtccgttcgc 960
agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg 1020
cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc 1080
gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga 1140
ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg 1200
gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg 1260
cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac 1320
cgaatcaccg acctctctcc ccagggggat ccaccggagc ttaccatgac cgagtacaag 1380
cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 1440
gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 1500
gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 1560
gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 1620
gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 1680
cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 1740
gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 1800
cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggaaac ctccgcgccc 1860
cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 1920
gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac 1980
ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cggtaccttt aagaccaatg 2040
acttacaagg cagctgtaga tcttagccac tttctagagt cggggcggcc ggccgcttcg 2100
agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa 2160
aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg 2220
caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt 2280
gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc 2340
gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca 2400
tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc 2460
cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 2520
cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 2580
gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 2640
ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 2700
agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 2760
tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 2820
ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag 2880
gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 2940
ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 3000
gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 3060
aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 3120
aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 3180
ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 3240
gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 3300
gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 3360
tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 3420
ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 3480
ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 3540
atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 3600
ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 3660
tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 3720
attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 3780
tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 3840
ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 3900
gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 3960
gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 4020
gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 4080
aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 4140
taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 4200
tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 4260
tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 4320
atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 4380
tttccccgaa aagtgccacc tgcaggaaac agctatgacc atgattacgc caagctctag 4440
ctagaggtcg acggtatcga gccccagctg gttctttccg cctcagaagc catagagccc 4500
accgcatccc cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc 4560
accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt 4620
tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg 4680
caaacaacag atggctggca actagaaggc acagtcgagg ctgatcagcg ggtttaaact 4740
caatggtgat ggtgatgatg accggttaga ctttcctctt cttcttggga gaaccaccag 4800
aggggggtgt ggcggtttcg ttgttcagtg tggggagtgt gagcagatca gactgctggg 4860
gggactctgg ccgggccccg ctcccctccc tcttgctggc gtcgtgccgc cccccccggc 4920
ttgtggtggg ccggatgctg gcccccctgg cgttcaaagt ctcccgccgg tcgcaggcca 4980
cgttcctatc aatcaacaca gcgtgcctct cgatgttcct agcggcgttc atatctctat 5040
ccatgaccag gccgcagtcg gtgcacctaa acaccctatc ggagagcttc aagtgggggt 5100
tctgccagcc gcacccgctg caggtcttgc tggaggggaa ccacctgtcg gccacggcca 5160
gctcgctccc gtaccaggtg gccttgtagt tcacctgcct ccgcagctcg gcaggggcgc 5220
tgtccaggat gacccggttc agcccggcct tctgccggac gttcttccct ggggcggcca 5280
ctgtccccct ggcggacctg gtcatgccgg cgacgttcag atcctccagg gccaccacgg 5340
cgaaccctgt ggtcagccgc ttggtgatct ggtgcagggc ggcgtgccgc cggacggcca 5400
cctcgtgatg ggccctcccc acccgccgct tggccttctc tctcctggcg ctgcccttct 5460
gtgtcctggc cagggcccgc tgggccttga gcagccgcct ctcagcctgc ctcaggtgct 5520
gggggtttct caccagggcg ctgcttgggt cgtcgacgac caggggcttg gacagggcgg 5580
ccagcacctt cacccccagg tccactccca cggcgcccct ctcccgctgc cgcctgctgg 5640
gccgatcggg cacctgcact gtcaccttgc agagcacgga ggcgtaccac ctgtgtccgc 5700
ccctactcac agtcacggac tgcaccacgg cgtcgcctct gtcaatgagc ctggcgagcc 5760
gcttgccgct gtcgtgcagc ctcacctcgc caatcctagg cagcctcagc cttctgtagc 5820
cagccagcct gatgctgggc ttcttcacat cgtggtgcaa tctaaagcta tcccgagccc 5880
ggcccttctt cttaaaccgg gggtagccca ccctcctccc cactctcttg ccgctcaggc 5940
tgtcgagcca gttcttccat gcctggtcgg cgtcaataaa ggcgctctgg aaggcgtagg 6000
tgttcacctc gtgccaccat gggcaggccc catccaggcc cttccgggaa tcccccttca 6060
cggcgttcag ggccttctgg atggctggct tcataggcac ggggacccgg acctgccgcc 6120
gggcctgctc ctcgggcacg ccatcgccca cgagcttggc cacctcggcc cgccaccgct 6180
cgtgggcccc caccttggcg gcgagggcgt ggttgaaggc ccacctggcg gccccggcgt 6240
gccggctcag ggcggccacc tggacggaag taggatcgag ggcgaaccga aaggccttca 6300
ggatctctgt ctgcaccatg accttcctct tcttcttagg ggccatggtg gcggctctcc 6360
ctatagtgag tcgtattagc ggccgcggat ctctagcgga tctgacggtt cactaaacca 6420
gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg 6480
gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg 6540
acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg 6600
atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc 6660
caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg 6720
tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg 6780
gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact 6840
atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc 6900
gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac 6960
cccgtaattg attactatta ataactagtc aataatcaat gtcaacgcgt atatctggcc 7020
cgtacatcgc gaagcagcgc aaaacgccta accctaagca gattcttcat gcaattgtcg 7080
gtcaagaatc gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac 7140
tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc tatcgata 7198
<210> 184
<211> 7198
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 184
ggtaccgatt agtgaacgga tctcgacggt atcgatcacg agactagcct cgagcggccg 60
cccccttcac cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg 120
ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata 180
cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa 240
tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct 300
tgtggaaagg acgaaacacc gaacgcggcc cggaacatcg aacgccacgc agtgctgatc 360
gatcgaaacg tcgcctgcga taggcgggag acgctaaacg cccgtggagc atccataaga 420
ccaaccacct ctcggggcgg taggcacgac gcatcgaagc gggaaggctc cggcgctcgg 480
cctgagtcac ctcagcagag tgatctgctg acgctcccaa ccttgaataa cgaaacggca 540
acgcctccat agcggtgcag gtcaataagg gtcggcccca cgcgtgtagg gagcgatcgt 600
tagcctggca tggtggtgct ttttttgaat tctcgacctc gagacaaatg gcagtattca 660
tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag 720
acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa 780
attttcgggt ttattacagg gacagcagag atccactttg gccgcggctc gagggggttg 840
gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt 900
ggttccggga aacgcagcgg cgccgaccct gggactcgca cattcttcac gtccgttcgc 960
agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg 1020
cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc 1080
gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga 1140
ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg 1200
gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg 1260
cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac 1320
cgaatcaccg acctctctcc ccagggggat ccaccggagc ttaccatgac cgagtacaag 1380
cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 1440
gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 1500
gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 1560
gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 1620
gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 1680
cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 1740
gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 1800
cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggaaac ctccgcgccc 1860
cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 1920
gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac 1980
ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cggtaccttt aagaccaatg 2040
acttacaagg cagctgtaga tcttagccac tttctagagt cggggcggcc ggccgcttcg 2100
agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa 2160
aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg 2220
caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt 2280
gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc 2340
gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca 2400
tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc 2460
cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 2520
cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 2580
gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 2640
ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 2700
agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 2760
tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 2820
ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag 2880
gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 2940
ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 3000
gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 3060
aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 3120
aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 3180
ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 3240
gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 3300
gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 3360
tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 3420
ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 3480
ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 3540
atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 3600
ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 3660
tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 3720
attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 3780
tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 3840
ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 3900
gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 3960
gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 4020
gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 4080
aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 4140
taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 4200
tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 4260
tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 4320
atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 4380
tttccccgaa aagtgccacc tgcaggaaac agctatgacc atgattacgc caagctctag 4440
ctagaggtcg acggtatcga gccccagctg gttctttccg cctcagaagc catagagccc 4500
accgcatccc cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc 4560
accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt 4620
tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg 4680
caaacaacag atggctggca actagaaggc acagtcgagg ctgatcagcg ggtttaaact 4740
caatggtgat ggtgatgatg accggttaga ctttcctctt cttcttggga gaaccaccag 4800
aggggggtgt ggcggtttcg ttgttcagtg tggggagtgt gagcagatca gactgctggg 4860
gggactctgg ccgggccccg ctcccctccc tcttgctggc gtcgtgccgc cccccccggc 4920
ttgtggtggg ccggatgctg gcccccctgg cgttcaaagt ctcccgccgg tcgcaggcca 4980
cgttcctatc aatcaacaca gcgtgcctct cgatgttcct agcggcgttc atatctctat 5040
ccatgaccag gccgcagtcg gtgcacctaa acaccctatc ggagagcttc aagtgggggt 5100
tctgccagcc gcacccgctg caggtcttgc tggaggggaa ccacctgtcg gccacggcca 5160
gctcgctccc gtaccaggtg gccttgtagt tcacctgcct ccgcagctcg gcaggggcgc 5220
tgtccaggat gacccggttc agcccggcct tctgccggac gttcttccct ggggcggcca 5280
ctgtccccct ggcggacctg gtcatgccgg cgacgttcag atcctccagg gccaccacgg 5340
cgaaccctgt ggtcagccgc ttggtgatct ggtgcagggc ggcgtgccgc cggacggcca 5400
cctcgtgatg ggccctcccc acccgccgct tggccttctc tctcctggcg ctgcccttct 5460
gtgtcctggc cagggcccgc tgggccttga gcagccgcct ctcagcctgc ctcaggtgct 5520
gggggtttct caccagggcg ctgcttgggt cgtcgacgac caggggcttg gacagggcgg 5580
ccagcacctt cacccccagg tccactccca cggcgcccct ctcccgctgc cgcctgctgg 5640
gccgatcggg cacctgcact gtcaccttgc agagcacgga ggcgtaccac ctgtgtccgc 5700
ccctactcac agtcacggac tgcaccacgg cgtcgcctct gtcaatgagc ctggcgagcc 5760
gcttgccgct gtcgtgcagc ctcacctcgc caatcctagg cagcctcagc cttctgtagc 5820
cagccagcct gatgctgggc ttcttcacat cgtggtgcaa tctaaagcta tcccgagccc 5880
ggcccttctt cttaaaccgg gggtagccca ccctcctccc cactctcttg ccgctcaggc 5940
tgtcgagcca gttcttccat gcctggtcgg cgtcaataaa ggcgctctgg aaggcgtagg 6000
tgttcacctc gtgccaccat gggcaggccc catccaggcc cttccgggaa tcccccttca 6060
cggcgttcag ggccttctgg atggctggct tcataggcac ggggacccgg acctgccgcc 6120
gggcctgctc ctcgggcacg ccatcgccca cgagcttggc cacctcggcc cgccaccgct 6180
cgtgggcccc caccttggcg gcgagggcgt ggttgaaggc ccacctggcg gccccggcgt 6240
gccggctcag ggcggccacc tggacggaag taggatcgag ggcgaaccga aaggccttca 6300
ggatctctgt ctgcaccatg accttcctct tcttcttagg ggccatggtg gcggctctcc 6360
ctatagtgag tcgtattagc ggccgcggat ctctagcgga tctgacggtt cactaaacca 6420
gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg 6480
gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg 6540
acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg 6600
atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc 6660
caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg 6720
tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg 6780
gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact 6840
atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc 6900
gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac 6960
cccgtaattg attactatta ataactagtc aataatcaat gtcaacgcgt atatctggcc 7020
cgtacatcgc gaagcagcgc aaaacgccta accctaagca gattcttcat gcaattgtcg 7080
gtcaagaatc gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac 7140
tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc tatcgata 7198
<210> 185
<211> 7198
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 185
ggtaccgatt agtgaacgga tctcgacggt atcgatcacg agactagcct cgagcggccg 60
cccccttcac cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg 120
ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata 180
cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa 240
tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct 300
tgtggaaagg acgaaacacc gaacgcggcc cggaacatcg aacgccacgc agtgctgatc 360
gatcgaaacg tcgcctgcga taggcgggag acgctaaacg cccgtggagc atccataaga 420
ccaaccacct ctcggggcgg taggcacgac gcatcgaagc gggaaggctc cggcgctcgg 480
cctgagtcac ctcagcagag tgatctgctg acgctcccaa ccttgaataa cgaaacggca 540
acgcctccat agcggtgcag gtcaataagg gtcggcccca cgcgtgtagg gagcgatcgt 600
taatttctaa ttccggaatt ttttttgaat tctcgacctc gagacaaatg gcagtattca 660
tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag 720
acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa 780
attttcgggt ttattacagg gacagcagag atccactttg gccgcggctc gagggggttg 840
gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt 900
ggttccggga aacgcagcgg cgccgaccct gggactcgca cattcttcac gtccgttcgc 960
agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg 1020
cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc 1080
gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga 1140
ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg 1200
gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg 1260
cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac 1320
cgaatcaccg acctctctcc ccagggggat ccaccggagc ttaccatgac cgagtacaag 1380
cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 1440
gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 1500
gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 1560
gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 1620
gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 1680
cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 1740
gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 1800
cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggaaac ctccgcgccc 1860
cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 1920
gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac 1980
ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cggtaccttt aagaccaatg 2040
acttacaagg cagctgtaga tcttagccac tttctagagt cggggcggcc ggccgcttcg 2100
agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa 2160
aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg 2220
caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt 2280
gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc 2340
gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca 2400
tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc 2460
cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 2520
cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 2580
gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 2640
ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 2700
agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 2760
tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 2820
ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag 2880
gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 2940
ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 3000
gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 3060
aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 3120
aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 3180
ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 3240
gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 3300
gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 3360
tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 3420
ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 3480
ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 3540
atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 3600
ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 3660
tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 3720
attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 3780
tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 3840
ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 3900
gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 3960
gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 4020
gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 4080
aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 4140
taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 4200
tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 4260
tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 4320
atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 4380
tttccccgaa aagtgccacc tgcaggaaac agctatgacc atgattacgc caagctctag 4440
ctagaggtcg acggtatcga gccccagctg gttctttccg cctcagaagc catagagccc 4500
accgcatccc cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc 4560
accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt 4620
tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg 4680
caaacaacag atggctggca actagaaggc acagtcgagg ctgatcagcg ggtttaaact 4740
caatggtgat ggtgatgatg accggttaga ctttcctctt cttcttggga gaaccaccag 4800
aggggggtgt ggcggtttcg ttgttcagtg tggggagtgt gagcagatca gactgctggg 4860
gggactctgg ccgggccccg ctcccctccc tcttgctggc gtcgtgccgc cccccccggc 4920
ttgtggtggg ccggatgctg gcccccctgg cgttcaaagt ctcccgccgg tcgcaggcca 4980
cgttcctatc aatcaacaca gcgtgcctct cgatgttcct agcggcgttc atatctctat 5040
ccatgaccag gccgcagtcg gtgcacctaa acaccctatc ggagagcttc aagtgggggt 5100
tctgccagcc gcacccgctg caggtcttgc tggaggggaa ccacctgtcg gccacggcca 5160
gctcgctccc gtaccaggtg gccttgtagt tcacctgcct ccgcagctcg gcaggggcgc 5220
tgtccaggat gacccggttc agcccggcct tctgccggac gttcttccct ggggcggcca 5280
ctgtccccct ggcggacctg gtcatgccgg cgacgttcag atcctccagg gccaccacgg 5340
cgaaccctgt ggtcagccgc ttggtgatct ggtgcagggc ggcgtgccgc cggacggcca 5400
cctcgtgatg ggccctcccc acccgccgct tggccttctc tctcctggcg ctgcccttct 5460
gtgtcctggc cagggcccgc tgggccttga gcagccgcct ctcagcctgc ctcaggtgct 5520
gggggtttct caccagggcg ctgcttgggt cgtcgacgac caggggcttg gacagggcgg 5580
ccagcacctt cacccccagg tccactccca cggcgcccct ctcccgctgc cgcctgctgg 5640
gccgatcggg cacctgcact gtcaccttgc agagcacgga ggcgtaccac ctgtgtccgc 5700
ccctactcac agtcacggac tgcaccacgg cgtcgcctct gtcaatgagc ctggcgagcc 5760
gcttgccgct gtcgtgcagc ctcacctcgc caatcctagg cagcctcagc cttctgtagc 5820
cagccagcct gatgctgggc ttcttcacat cgtggtgcaa tctaaagcta tcccgagccc 5880
ggcccttctt cttaaaccgg gggtagccca ccctcctccc cactctcttg ccgctcaggc 5940
tgtcgagcca gttcttccat gcctggtcgg cgtcaataaa ggcgctctgg aaggcgtagg 6000
tgttcacctc gtgccaccat gggcaggccc catccaggcc cttccgggaa tcccccttca 6060
cggcgttcag ggccttctgg atggctggct tcataggcac ggggacccgg acctgccgcc 6120
gggcctgctc ctcgggcacg ccatcgccca cgagcttggc cacctcggcc cgccaccgct 6180
cgtgggcccc caccttggcg gcgagggcgt ggttgaaggc ccacctggcg gccccggcgt 6240
gccggctcag ggcggccacc tggacggaag taggatcgag ggcgaaccga aaggccttca 6300
ggatctctgt ctgcaccatg accttcctct tcttcttagg ggccatggtg gcggctctcc 6360
ctatagtgag tcgtattagc ggccgcggat ctctagcgga tctgacggtt cactaaacca 6420
gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg 6480
gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg 6540
acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg 6600
atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc 6660
caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg 6720
tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg 6780
gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact 6840
atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc 6900
gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac 6960
cccgtaattg attactatta ataactagtc aataatcaat gtcaacgcgt atatctggcc 7020
cgtacatcgc gaagcagcgc aaaacgccta accctaagca gattcttcat gcaattgtcg 7080
gtcaagaatc gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac 7140
tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc tatcgata 7198
<210> 186
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 186
ctggcaagta gtaaaatgct 20
<210> 187
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 187
gcttcctcaa atcttatgca 20
<210> 188
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 188
gatgtactag ctgggctgtt ct 22
<210> 189
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 189
ggaagctctg tccaaaccat ct 22
<210> 190
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 190
gaagagtttt ctgtggtgtg agc 23
<210> 191
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 191
gcctcttata ggagactgaa ggc 23
<210> 192
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 192
ctagtatctc gccaacctga cc 22
<210> 193
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 193
gtgaagccaa tgaccacaga ac 22
<210> 194
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 194
tttagtgcag cttctgccgt ta 22
<210> 195
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 195
acgcaactga caggaggaat tg 22

Claims (20)

1.C2C9核酸酶用作RNA引导的核酸内切酶的用途。
2.C2C9核酸酶或编码所述C2C9核酸酶的核酸在基因编辑系统中的用途。
3.一种基因编辑系统，其特征在于，所述基因编辑系统包括C2C9核酸酶和/或编码所述C2C9核酸酶的核酸，以及引导RNA或编码所述引导RNA的核酸。
4.如权利要求3所述的基因编辑系统，其特征在于，所述C2C9核酸酶是：
(I)野生型C2C9核酸酶或其片段，具有受RNA引导的核酸结合活性；
(II)与(I)的氨基酸序列具有至少50％序列同源性的变体，且具有受RNA引导的核酸结合活性；
(III)根据(I)或(II)，其进一步包括核定位信号片段；
(IV)根据(I)或(II)或(III)，其进一步包含：
(a)一种或多种修饰或突变，其产生具有相比修饰或突变前显著减小的核酸内切酶活性，或使核酸内切酶活性丧失；和/或
(b)具有其他功能活性的多肽或结构域；
(V)根据(I)或(II)或(III)，所述C2C9核酸酶具有核酸内切酶活性。
5.如权利要求3或4所述的基因编辑系统，其特征在于，所述C2C9核酸酶不超过800个氨基酸；和/或，所述C2C9核酸酶具有受RNA引导的核酸结合活性。
6.如权利要求3-5任一所述的基因编辑系统，其特征在于，所述的基因编辑系统识别靶向序列上的PAM序列；和/或，所述基因编辑系统靶向PAM序列之后长度为12～40bp的核酸片段，优选的长度为20bp。
7.如权利要求6所述的基因编辑系统，其特征在于，所述PAM序列为AAN，GAN；其中N为简并碱基，代表A、T、C或G任意碱基。
8.如权利要求3-7任一所述的基因编辑系统，其特征在于，所述的C2C9核酸酶从N端到C端包含：
结构域1，其包含如SEQ ID NO.1所示的氨基酸序列或与该氨基酸序列具有至少50％序列同一性的变体；具有受RNA引导的核酸结合活性和/或核酸内切酶活性；
结构域2，其包含如SEQ ID NO.2所示的氨基酸序列或与该氨基酸序列具有至少50％序列同一性的变体，且具有受RNA引导的核酸结合活性和/或核酸内切酶活性。
9.如权利要求3-7任一所述的基因编辑系统，其特征在于，所述C2C9核酸酶为SEQ IDNO.3-117所示的氨基酸序列之任一或与SEQ ID NO.3-117所示的任一氨基酸序列具有至少50％序列同源性，且具有受RNA引导的核酸结合活性和/或核酸内切酶活性。
10.如权利要求3-7任一所述的基因编辑系统，其特征在于，所述引导RNA包含：
能够与靶序列杂交的基因靶向区段(i)，
tracr配对物序列(ii)，
tracr RNA序列(iii)；
其中，所述tracr配对物序列(ii)与所述tracrRNA序列(iii)杂交，并形成茎-环结构；
所述引导RNA为一条链，其由所述核酸靶向区段(i)与tracr配对物序列(ii)和tracrRNA序列(iii)依次连接形成；或，所述引导RNA包括两条链，其中一条链由所述基因靶向区段(i)与tracr配对物序列(ii)连接形成，另一条链为所述tracrRNA序列(iii)。
11.如权利要求5或6所述的基因编辑系统，其特征在于，所述基因编辑系统包括：
(1)C2C9核酸酶的表达构建体；
(2)完整引导RNA的表达构建体；
所述完整引导RNA包含引导RNA骨架，以及在该引导RNA骨架的3’末端的添加的靶向序列所对应的RNA序列；所述靶向序列为PAM序列之后长度为12～40bp的核酸片段，优选为PAM序列之后长度为20bp的核酸片段。
12.如权利要求10所述的基因编辑系统，其特征在于，所述引导RNA序列与所述C2C9核酸酶相对应，其中所述Actinomadura craniellae C2C9(SEQ ID NO.3)所对应的tracr配对物序列如SEQ ID NO.118所示，所述tracr RNA序列如SEQ ID NO.119所示，连接在一起得到的引导RNA骨架序列如SEQ ID NO.120所示。
13.一种基因编辑方法，其特征在于，将靶基因与所述权利要求3-12任一所述的基因编辑系统接触，以实现靶基因的编辑。
14.如权利要求13所述的基因编辑方法，其特征在于，包括下列步骤：
i)将所述C2C9核酸酶或编码所述C2C9核酸酶的核酸引入细胞中；
ii)将所述引导RNA或编码所述引导RNA的核酸引入细胞；
III)由C2C9核酸酶介导，在靶基因中产生一种或多种切口，或靶向、编辑、修饰或操纵所述靶基因。
15.如权利要求13或14所述的基因编辑方法，其特征在于，所述C2C9核酸酶通过加工或未加工形式的引导RNA引导至靶基因。
16.如权利要求13或14所述的基因编辑方法，其特征在于，所述C2C9核酸酶和引导RNA形成复合物，识别所述靶基因上的PAM序列；和/或，所述基因编辑系统的靶向序列为PAM序列之后长度为12～40bp的核酸片段，优选PAM序列之后长度为20bp的核酸片段。
17.如权利要求14所述的基因编辑方法，其特征在于，所述方法进一步包括将包含异源多核苷酸序列的供体模板引入细胞中的步骤。
18.如权利要求3-12任一所述的基因编辑系统或如权利要求13-17任一所述的方法在体内、离体细胞或无细胞环境中对靶基因和/或其相关多肽进行基因编辑中的应用，其特征在于，所述离体细胞包括细菌细胞、古细菌细胞、真菌细胞、原生生物细胞、病毒细胞、植物细胞和动物细胞。
19.如权利要求3-12任一所述的基因编辑系统或如权利要求13-17任一所述的方法在体内、离体细胞或无细胞环境中对靶基因和/或其相关多肽进行基因编辑中的应用，其特征在于，其特征在于，所述的基因编辑选自由：基因切割、基因删除、基因插入、点突变、转录抑制、转录激活、碱基编辑和引导编辑构成的群组。
20.一种遗传修饰的细胞，其通过如上如权利要求3-12任一所述的基因编辑系统或如权利要求13-17任一所述的方法进行基因编辑获得。
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WO2024125221A1 (zh) * 2022-12-15 2024-06-20 上海科技大学 一种碱基编辑系统及碱基编辑方法

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