JP7116062B2 - 多重化シグナル増幅 - Google Patents

多重化シグナル増幅 Download PDF

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JP7116062B2
JP7116062B2 JP2019531088A JP2019531088A JP7116062B2 JP 7116062 B2 JP7116062 B2 JP 7116062B2 JP 2019531088 A JP2019531088 A JP 2019531088A JP 2019531088 A JP2019531088 A JP 2019531088A JP 7116062 B2 JP7116062 B2 JP 7116062B2
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strand
probe
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JP2020503856A (ja
JP2020503856A5 (https=
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キシ，ジョスリン，ヨシコ
ベリヴォー，ブライアン
イン，ペン
ワン，ユー
サカ，シネム，ケイ．
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Harvard University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation

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US201762444734P 2017-01-10 2017-01-10
US62/444,734 2017-01-10
US201762546418P 2017-08-16 2017-08-16
US62/546,418 2017-08-16
US201762546836P 2017-08-17 2017-08-17
US62/546,836 2017-08-17
PCT/US2018/013019 WO2018132392A2 (en) 2017-01-10 2018-01-09 Multiplexed signal amplification

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JP2020503856A JP2020503856A (ja) 2020-02-06
JP2020503856A5 JP2020503856A5 (https=) 2021-02-25
JP7116062B2 true JP7116062B2 (ja) 2022-08-09

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EP (1) EP3568492B1 (https=)
JP (1) JP7116062B2 (https=)
CN (1) CN110168101A (https=)
WO (1) WO2018132392A2 (https=)

Families Citing this family (117)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2011319755B2 (en) 2010-10-27 2017-02-23 President And Fellows Of Harvard College Compositions of toehold primer duplexes and methods of use
ES2891085T3 (es) 2015-01-30 2022-01-26 Harvard College Obtención de imágenes sin microscopio
CN116949140A (zh) 2016-02-17 2023-10-27 哈佛学院院长及董事 分子编程工具
EP3448886A4 (en) 2016-04-26 2019-12-11 Ultivue, Inc. SUPER-HIGH-RESOLUTION IMMUNOFLUORESCENCE WITH BINDING-LIMITED PREVIEW
EP4050112A1 (en) 2016-06-21 2022-08-31 10X Genomics, Inc. Nucleic acid sequencing
EP3568492B1 (en) * 2017-01-10 2025-01-01 President and Fellows of Harvard College Multiplexed signal amplification
CA3078158A1 (en) 2017-10-06 2019-04-11 Cartana Ab Rna templated ligation
CN111630179B (zh) 2018-01-26 2024-07-19 哈佛学院院长及董事 邻近检测方法和组合物
WO2019236841A1 (en) 2018-06-08 2019-12-12 Ultivue, Inc. Multiplexed catalyzed reporter deposition
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
WO2020069386A2 (en) * 2018-09-28 2020-04-02 California Institute Of Technology Efficient combinatorial bead barcoding
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
EP3894590B1 (en) 2018-12-10 2025-10-22 10X Genomics, Inc. Methods of using master / copy arrays for spatial detection
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
WO2020240025A1 (en) 2019-05-31 2020-12-03 Cartana Ab Method of detecting target nucleic acid molecules
US20210072143A1 (en) * 2019-07-01 2021-03-11 Davinci Bio, Inc. High capacity molecule detection
US20220356509A1 (en) 2019-07-05 2022-11-10 Ultivue, Inc. Improved Multiplexing Method
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
CN110714049B (zh) * 2019-11-12 2021-07-02 北京理工大学 一种检测生物标志物的微生物传感器及检测方法、培养与检测芯片和检测系统
GB201919032D0 (en) 2019-12-20 2020-02-05 Cartana Ab Method of detecting an analyte
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
WO2021133849A1 (en) 2019-12-23 2021-07-01 10X Genomics, Inc. Methods for spatial analysis using rna-templated ligation
CN115135984B (zh) 2019-12-23 2025-12-23 10X基因组学有限公司 可逆固定试剂及其使用方法
WO2021138312A1 (en) 2019-12-30 2021-07-08 Ultivue, Inc. Methods for reducing nonspecific interactions on biological samples
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110548B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Bi-directional in situ analysis
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
EP4107284A1 (en) 2020-02-17 2022-12-28 10X Genomics, Inc. In situ analysis of chromatin interaction
EP4484571A3 (en) 2020-02-21 2025-03-19 10X Genomics, Inc. Methods and compositions for integrated in situ spatial assay
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
ES2965354T3 (es) 2020-04-22 2024-04-12 10X Genomics Inc Métodos para análisis espacial que usan eliminación de ARN elegido como diana
EP4553168A3 (en) 2020-05-22 2025-08-27 10x Genomics, Inc. Spatial analysis to detect sequence variants
EP4153775B1 (en) 2020-05-22 2024-07-24 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
EP4025692A2 (en) 2020-06-02 2022-07-13 10X Genomics, Inc. Nucleic acid library methods
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
US12209273B2 (en) 2020-06-12 2025-01-28 10X Genomics, Inc. Nucleic acid assays using click chemistry bioconjugation
EP4172362B1 (en) 2020-06-25 2024-09-18 10X Genomics, Inc. Spatial analysis of dna methylation
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US12553898B1 (en) 2020-08-10 2026-02-17 10X Genomics, Inc. Fluorescent hybridization of antibody-oligonucleotide for multiplexing and signal amplification
US12297499B2 (en) 2020-08-17 2025-05-13 10X Genomics, Inc. Multicomponent nucleic acid probes for sample analysis
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
CN114544937B (zh) * 2020-11-11 2023-04-07 艾克发(北京)生物技术有限公司 一种多重信号放大系统及其在免疫吸附间接法检测中的应用
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4012046A1 (en) 2020-12-11 2022-06-15 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
EP4729631A2 (en) 2020-12-21 2026-04-22 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
US12275984B2 (en) 2021-03-02 2025-04-15 10X Genomics, Inc. Sequential hybridization and quenching
EP4428246B1 (en) 2021-04-14 2025-12-24 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
CN113156122B (zh) * 2021-04-29 2023-07-04 重庆医科大学 一种检测外泌体pd-l1的荧光传感器及其制备与应用
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
US20220380838A1 (en) 2021-06-01 2022-12-01 10X Genomics, Inc. Methods and compositions for analyte detection and probe resolution
WO2022256422A1 (en) 2021-06-02 2022-12-08 10X Genomics, Inc. Sample analysis using asymmetric circularizable probes
EP4582555A3 (en) 2021-06-03 2025-10-22 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
CN113340864B (zh) * 2021-06-07 2023-04-07 郑州轻工业大学 基于mef效应二次放大ins信号的适体传感器及其制备方法和应用
WO2023283442A1 (en) 2021-07-09 2023-01-12 10X Genomics, Inc. Methods for detecting analytes using sparse labelling
CN117651855A (zh) 2021-07-13 2024-03-05 10X基因组学有限公司 用于制备具有可控厚度的聚合基质的方法
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
CA3224093A1 (en) 2021-07-30 2023-02-02 Jorge Ivan Hernandez Neuta Methods and compositions for synchronizing reactions in situ
US12460251B2 (en) 2021-08-03 2025-11-04 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid molecules
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
EP4381095A1 (en) 2021-08-03 2024-06-12 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
US12529096B2 (en) 2021-08-03 2026-01-20 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid structures
US12435364B2 (en) 2021-08-16 2025-10-07 10X Genomics, Inc. Probes comprising a split barcode region and methods of use
ES3011462T3 (en) 2021-09-01 2025-04-07 10X Genomics Inc Methods for blocking a capture probe on a spatial array
CN114540345B (zh) * 2021-11-03 2024-04-09 武汉蓝沙医学检验实验室有限公司 一种发夹结构的标签荧光探针和荧光检测方法
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
EP4305195A2 (en) 2021-12-01 2024-01-17 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
WO2023108139A2 (en) 2021-12-10 2023-06-15 10X Genomics, Inc. Multi-resolution in situ decoding
WO2023129898A2 (en) 2021-12-27 2023-07-06 10X Genomics, Inc. Methods and compositions for rolling circle amplification
US20250101511A1 (en) * 2022-01-17 2025-03-27 University Of Washington Oligo-mediated deposition of functional molecules for spatially resolved genomics, transcriptomics, and proteomics
EP4733410A2 (en) 2022-01-21 2026-04-29 10X Genomics, Inc. Multiple readout signals for analyzing a sample
WO2023154709A2 (en) * 2022-02-08 2023-08-17 The Trustees Of The University Of Pennsylvania Methods for rapid, scalable, amplified nucleic acid detection in situ
EP4490737A1 (en) 2022-03-08 2025-01-15 10X Genomics, Inc. In situ code design methods for minimizing optical crowding
EP4500481A1 (en) 2022-03-29 2025-02-05 10X Genomics, Inc. Spectral unmixing combined with decoding for super-multiplexed in situ analysis
AU2023243205A1 (en) 2022-04-01 2024-09-19 10X Genomics, Inc. Compositions and methods for targeted masking of autofluorescence
CN119234045A (zh) 2022-04-06 2024-12-31 10X基因组学有限公司 用于多重细胞分析的方法
EP4519453A1 (en) 2022-05-06 2025-03-12 10X Genomics, Inc. Methods and compositions for in situ analysis of v(d)j sequences
WO2023215612A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Analysis of antigen and antigen receptor interactions
CN119213143A (zh) 2022-05-11 2024-12-27 10X基因组学有限公司 用于原位测序的组合物和方法
WO2023245190A1 (en) 2022-06-17 2023-12-21 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
US20260022413A1 (en) * 2022-07-20 2026-01-22 President And Fellows Of Harvard College Engineering dynamic dna nano-devices to amplify signal
WO2024040060A1 (en) 2022-08-16 2024-02-22 10X Genomics, Inc. Ap50 polymerases and uses thereof
EP4602344A1 (en) 2022-10-14 2025-08-20 10X Genomics, Inc. Methods for analysis of biological samples
WO2024102809A1 (en) 2022-11-09 2024-05-16 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample
WO2024137860A1 (en) 2022-12-21 2024-06-27 10X Genomics, Inc. Methods for tethering ribonucleic acids in biological samples
US20240368678A1 (en) 2023-05-03 2024-11-07 10X Genomics, Inc. Methods and compositions for spatial assay
US20240376521A1 (en) 2023-05-10 2024-11-14 10X Genomics, Inc. Boronic acid compositions and methods for tethering ribonucleic acids in biological samples
WO2025014758A1 (en) 2023-07-07 2025-01-16 10X Genomics, Inc. Systems and methods for tissue bounds detection
WO2025029831A1 (en) 2023-07-31 2025-02-06 10X Genomics, Inc. Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification
WO2025038914A1 (en) 2023-08-16 2025-02-20 10X Genomics, Inc. Systems and methods for image segmentation
WO2025076421A1 (en) 2023-10-06 2025-04-10 10X Genomics, Inc. Feature pyramiding for in situ data visualizations
US20250163502A1 (en) 2023-11-22 2025-05-22 10X Genomics, Inc. Methods for processing ribonucleic acids in biological samples
US20250188524A1 (en) 2023-12-07 2025-06-12 10X Genomics, Inc. Graphical user interface and method of estimating an instrument run completion time
WO2025160489A1 (en) 2024-01-25 2025-07-31 10X Genomics, Inc. Systems and methods for image segmentation using multiple stain indicators
WO2025189080A1 (en) 2024-03-08 2025-09-12 10X Genomics, Inc. Multi-focus image fusion with background removal
WO2025255457A1 (en) 2024-06-07 2025-12-11 10X Genomics, Inc. Compositions and methods for improved on-target spatial profiling
WO2026068590A1 (en) 2024-09-26 2026-04-02 Universite De Fribourg METHODS OF DETECTION OF TARGET RNAs IN A SAMPLE AND USES THEREOF
WO2026076066A1 (en) 2024-10-01 2026-04-09 10X Genomics, Inc. Systems and methods for illumination of a sample
CN120829971A (zh) * 2025-07-15 2025-10-24 湖南工程学院 一种基于双向引物交换反应检测卵巢癌miRNA的荧光传感探针体系及方法
CN120796476B (zh) * 2025-07-24 2026-04-14 上海市第十人民医院 一种用于循环肿瘤细胞标志蛋白多重分析的锁钥型dna组装体

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002503948A (ja) 1995-11-21 2002-02-05 イェール ユニバーシティ 単分子セグメントの増幅および検出
US20030165917A1 (en) 2001-08-14 2003-09-04 Ullman Edwin F. Isothermal amplification in nucleic acid analysis
US20070003950A1 (en) 2001-10-10 2007-01-04 Superarray Bioscience Corporation Detecting targets by unique identifier nucleotide tags
WO2016123419A1 (en) 2015-01-30 2016-08-04 President And Fellows Of Harvard College Microscope-free imaging

Family Cites Families (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5030566A (en) * 1989-01-04 1991-07-09 Board Of Regents, The University Of Texas System Concatemeric DNA length standards
US5543507A (en) 1992-03-05 1996-08-06 Isis Pharmaceuticals, Inc. Covalently cross-linked oligonucleotides
US5681697A (en) * 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US5866336A (en) 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
WO2000034527A2 (en) 1998-12-11 2000-06-15 The Regents Of The University Of California Targeted molecular bar codes
ES2546450T3 (es) 2000-04-07 2015-09-23 Eiken Kagaku Kabushiki Kaisha Método para amplificar y detectar un ácido nucleico usando ácido nucleico bicatenario como molde
US7033758B2 (en) * 2000-06-02 2006-04-25 Bayer Corporation Highly sensitive gene detection and localization using in situ branched-DNA hybridization
US20040161741A1 (en) 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
AU2004232976B2 (en) 2003-04-18 2011-02-10 Becton, Dickinson And Company Immuno-amplification
NZ544959A (en) 2003-07-07 2009-02-28 One Cell Systems Inc Hairpin-labeled probes and methods of use
EP2308966A1 (en) 2003-10-10 2011-04-13 Novozymes A/S Protease variants
WO2005059157A2 (en) * 2003-12-11 2005-06-30 The Board Of Trustees Of The Leland Stanford Junior University METHODS AND COMPOSITIONS FOR USE IN PREPARING HAIRPIN RNAs
US20060057590A1 (en) * 2004-09-14 2006-03-16 Azeddine Si-Ammour RNA probes
CN101048505A (zh) 2004-10-25 2007-10-03 德福根有限公司 用于递送双链rna至有害生物的包含至少一个适配子的多结构域rna分子
AU2006204087B2 (en) 2005-01-03 2010-09-16 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Primer for nucleic acid detection
EP1896617B1 (en) 2005-05-31 2013-01-02 Life Technologies Corporation Multiplex amplification of short nucleic acids
US20070026430A1 (en) 2005-06-30 2007-02-01 Applera Corporation Proximity probing of target proteins comprising restriction and/or extension
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
EP2522751B1 (en) 2006-06-01 2018-11-21 Third Wave Technologies, Inc. Detection of nucleic acids by an improved INVADER assay
WO2010012464A1 (en) 2008-08-01 2010-02-04 Roche Diagnostics Gmbh Improved lysis and reverse transcription for mrna quantification
US20110294116A1 (en) 2008-12-11 2011-12-01 The Regents of the Unicersity of California Methods and systems for direct sequencing of single dna molecules
US8445199B2 (en) * 2008-12-31 2013-05-21 Abbott Point Of Care Inc. Method and device for immunoassay using nucleotide conjugates
GB0910302D0 (en) * 2009-06-15 2009-07-29 Lumora Ltd Nucleic acid amplification
WO2011066330A2 (en) 2009-11-24 2011-06-03 Life Technologies Corporation Selective amplification of polynucleotide sequences
EP2524056A4 (en) 2010-01-15 2013-08-14 Univ British Columbia MULTIPLEX REINFORCEMENT FOR DETECTING NUCLEIC ACID VARIATIONS
EP2576839B1 (en) 2010-06-07 2017-05-10 Firefly Bioworks, Inc. Nucleic acid detection and quantification by post-hybridization labeling and universal encoding
US8962241B2 (en) 2010-07-20 2015-02-24 California Institute Of Technology Triggered molecular geometry based bioimaging probes
US9834439B2 (en) 2010-07-20 2017-12-05 California Institute Of Technology Biomolecular self-assembly
AU2011319755B2 (en) 2010-10-27 2017-02-23 President And Fellows Of Harvard College Compositions of toehold primer duplexes and methods of use
EP2633080B1 (en) 2010-10-29 2018-12-05 President and Fellows of Harvard College Method of detecting targets using fluorescently labelled nucleic acid nanotube probes
EP2633081B1 (en) * 2010-10-29 2017-01-11 Olink Bioscience AB Proximity ligation technology for western blot applications
CN103502448B (zh) 2010-11-12 2017-03-29 Gen9股份有限公司 核酸合成的方法和设备
US20130123121A1 (en) 2010-11-22 2013-05-16 The University Of Chicago Methods and/or Use of Oligonucleotide-Bead Conjugates for Assays and Detections
US8759036B2 (en) 2011-03-21 2014-06-24 Affymetrix, Inc. Methods for synthesizing pools of probes
WO2013012434A1 (en) 2011-07-15 2013-01-24 University Of Miami Bioluminescent stem-loop probes, compositions containing the same and methods utilizing the same
JP6226869B2 (ja) 2011-10-21 2017-11-08 オックスフォード ナノポール テクノロジーズ リミテッド 酵素法
AU2013202354A1 (en) 2012-06-18 2014-01-16 Speedx Pty Ltd Target detection and signal amplification
CN103014168A (zh) 2012-12-28 2013-04-03 北京大学 基于DNA hairpin和RCA的核酸检测方法
EP3988671A1 (en) 2013-02-20 2022-04-27 Emory University Compositions for sequencing nucleic acids in mixtures
CA2907493A1 (en) * 2013-04-30 2014-11-13 California Institute Of Technology Multiplex labeling of molecules by sequential hybridization barcoding
CN106062211A (zh) 2013-12-15 2016-10-26 哈佛学院院长等 与光学超分辨率图案化相关的方法和组合物
WO2015095633A1 (en) 2013-12-20 2015-06-25 Board Of Regents, The University Of Texas System Methods and compositions related to nucleic acid circuits and signal transducers
JP6739339B2 (ja) 2014-01-15 2020-08-12 アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ カバーされた配列変換ｄｎａおよび検出方法
GB201401885D0 (en) * 2014-02-04 2014-03-19 Olink Ab Proximity assay with detection based on hybridisation chain reaction (HCR)
WO2015178978A2 (en) 2014-02-14 2015-11-26 The Board Of Regents Of The University Of Texas System Strand exchange hairpin primers that give high allelic discrimination
CN104164488B (zh) 2014-07-09 2016-11-02 青岛艾菲生物技术有限公司 一种单引物引发的核酸恒温扩增方法
WO2016140726A2 (en) 2014-12-16 2016-09-09 President And Fellows Of Harvard College Triggered assembly of metafluorophores
AU2016252998B2 (en) * 2015-04-24 2021-11-04 Atila Biosystems Incorporated Amplification with primers of limited nucleotide composition
KR20180085717A (ko) * 2015-09-24 2018-07-27 에이비비트로, 엘엘씨 친화성-올리고뉴클레오타이드 콘쥬게이트 및 그것의 사용
CN116949140A (zh) 2016-02-17 2023-10-27 哈佛学院院长及董事 分子编程工具
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
US11208676B2 (en) 2016-05-27 2021-12-28 President And Fellows Of Harvard College Conditional primer extension for single-molecule detection
PT3464628T (pt) * 2016-06-06 2024-06-03 Redvault Biosciences Lp Construções repórter de alvo e suas utilizações
CA3034617A1 (en) * 2016-08-30 2018-03-08 California Institute Of Technology Immunohistochemistry via hybridization chain reaction
US10697006B2 (en) * 2016-09-15 2020-06-30 Agilent Technologies, Inc. Hairpin-mediated amplification method
CN109923213B (zh) * 2016-09-20 2023-02-28 哈佛学院院长及董事 分子验证系统
EP3568492B1 (en) * 2017-01-10 2025-01-01 President and Fellows of Harvard College Multiplexed signal amplification
WO2018175296A2 (en) * 2017-03-20 2018-09-27 President And Fellows Of Harvard College Programmable nucleic acid synthesis cascade
CN111630179B (zh) * 2018-01-26 2024-07-19 哈佛学院院长及董事 邻近检测方法和组合物
US11841371B2 (en) 2018-03-13 2023-12-12 The Broad Institute, Inc. Proteomics and spatial patterning using antenna networks
WO2020069386A2 (en) * 2018-09-28 2020-04-02 California Institute Of Technology Efficient combinatorial bead barcoding

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002503948A (ja) 1995-11-21 2002-02-05 イェール ユニバーシティ 単分子セグメントの増幅および検出
US20030165917A1 (en) 2001-08-14 2003-09-04 Ullman Edwin F. Isothermal amplification in nucleic acid analysis
US20070003950A1 (en) 2001-10-10 2007-01-04 Superarray Bioscience Corporation Detecting targets by unique identifier nucleotide tags
WO2016123419A1 (en) 2015-01-30 2016-08-04 President And Fellows Of Harvard College Microscope-free imaging

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