JP6665088B2 - 配列操作のための最適化されたＣＲＩＳＰＲ−Ｃａｓ二重ニッカーゼ系、方法および組成物 - Google Patents

配列操作のための最適化されたＣＲＩＳＰＲ−Ｃａｓ二重ニッカーゼ系、方法および組成物 Download PDF

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JP6665088B2
JP6665088B2 JP2016521450A JP2016521450A JP6665088B2 JP 6665088 B2 JP6665088 B2 JP 6665088B2 JP 2016521450 A JP2016521450 A JP 2016521450A JP 2016521450 A JP2016521450 A JP 2016521450A JP 6665088 B2 JP6665088 B2 JP 6665088B2
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sequence
composition
tracr
crispr
guide
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JP2016521994A (ja
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フェン チャン
フェン チャン
パトリック スー
パトリック スー
チェ ユ リン
チェ ユ リン
フェイ ラン
フェイ ラン
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Massachusetts Institute of Technology
Broad Institute Inc
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Broad Institute Inc
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    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N15/1034—Isolating an individual clone by screening libraries
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2016521450A 2013-06-17 2014-06-10 配列操作のための最適化されたＣＲＩＳＰＲ−Ｃａｓ二重ニッカーゼ系、方法および組成物 Active JP6665088B2 (ja)

Applications Claiming Priority (13)

Application Number Priority Date Filing Date Title
US201361836123P 2013-06-17 2013-06-17
US61/836,123 2013-06-17
US201361847537P 2013-07-17 2013-07-17
US61/847,537 2013-07-17
US201361862355P 2013-08-05 2013-08-05
US61/862,355 2013-08-05
US201361871301P 2013-08-28 2013-08-28
US61/871,301 2013-08-28
US201361915383P 2013-12-12 2013-12-12
USPCT/US2013/074667 2013-12-12
US61/915,383 2013-12-12
PCT/US2013/074667 WO2014093622A2 (en) 2012-12-12 2013-12-12 Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications
PCT/US2014/041803 WO2014204725A1 (en) 2013-06-17 2014-06-10 Optimized crispr-cas double nickase systems, methods and compositions for sequence manipulation

Related Child Applications (1)

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JP2020025947A Division JP7083364B2 (ja) 2013-06-17 2020-02-19 配列操作のための最適化されたＣＲＩＳＰＲ－Ｃａｓ二重ニッカーゼ系、方法および組成物

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JP6665088B2 true JP6665088B2 (ja) 2020-03-13

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JP2020025947A Active JP7083364B2 (ja) 2013-06-17 2020-02-19 配列操作のための最適化されたＣＲＩＳＰＲ－Ｃａｓ二重ニッカーゼ系、方法および組成物
JP2022031416A Active JP7383062B2 (ja) 2013-06-17 2022-03-02 配列操作のための最適化されたＣＲＩＳＰＲ－Ｃａｓ二重ニッカーゼ系、方法および組成物
JP2023189768A Active JP7682975B2 (ja) 2013-06-17 2023-11-07 配列操作のための最適化されたＣＲＩＳＰＲ－Ｃａｓ二重ニッカーゼ系、方法および組成物

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JP2022031416A Active JP7383062B2 (ja) 2013-06-17 2022-03-02 配列操作のための最適化されたＣＲＩＳＰＲ－Ｃａｓ二重ニッカーゼ系、方法および組成物
JP2023189768A Active JP7682975B2 (ja) 2013-06-17 2023-11-07 配列操作のための最適化されたＣＲＩＳＰＲ－Ｃａｓ二重ニッカーゼ系、方法および組成物

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US (3) US10711285B2 (https=)
EP (2) EP3011030B1 (https=)
JP (4) JP6665088B2 (https=)
KR (1) KR20160034901A (https=)
CN (1) CN105492611A (https=)
AU (1) AU2014281027A1 (https=)
CA (1) CA2915837A1 (https=)
WO (1) WO2014204725A1 (https=)

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