JP6642705B2 - 顕微鏡 - Google Patents

顕微鏡 Download PDF

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Publication number
JP6642705B2
JP6642705B2 JP2018518027A JP2018518027A JP6642705B2 JP 6642705 B2 JP6642705 B2 JP 6642705B2 JP 2018518027 A JP2018518027 A JP 2018518027A JP 2018518027 A JP2018518027 A JP 2018518027A JP 6642705 B2 JP6642705 B2 JP 6642705B2
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Prior art keywords
light
wavelength
microscope
shielding plate
lvf
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Japanese (ja)
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JPWO2017199407A1 (ja
Inventor
翔大 土田
翔大 土田
圭 堀江
圭 堀江
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Nikon Corp
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Nikon Corp
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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0064—Optical details of the image generation multi-spectral or wavelength-selective arrangements, e.g. wavelength fan-out, chromatic profiling
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/361—Optical details, e.g. image relay to the camera or image sensor
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/368—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements details of associated display arrangements, e.g. mounting of LCD monitor
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B26/00—Optical devices or arrangements for the control of light using movable or deformable optical elements
    • G02B26/007—Optical devices or arrangements for the control of light using movable or deformable optical elements the movable or deformable optical element controlling the colour, i.e. a spectral characteristic, of the light
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G01N2021/6471—Special filters, filter wheel
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B26/00—Optical devices or arrangements for the control of light using movable or deformable optical elements
    • G02B26/08—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light
    • G02B26/10—Scanning systems
    • G02B26/105—Scanning systems with one or more pivoting mirrors or galvano-mirrors

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  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Astronomy & Astrophysics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Spectrometry And Color Measurement (AREA)
JP2018518027A 2016-05-19 2016-05-19 顕微鏡 Active JP6642705B2 (ja)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2016/064932 WO2017199407A1 (ja) 2016-05-19 2016-05-19 顕微鏡

Publications (2)

Publication Number Publication Date
JPWO2017199407A1 JPWO2017199407A1 (ja) 2019-03-07
JP6642705B2 true JP6642705B2 (ja) 2020-02-12

Family

ID=60326528

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2018518027A Active JP6642705B2 (ja) 2016-05-19 2016-05-19 顕微鏡

Country Status (4)

Country Link
US (1) US10914932B2 (zh)
JP (1) JP6642705B2 (zh)
CN (1) CN109313326B (zh)
WO (1) WO2017199407A1 (zh)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102450912B1 (ko) * 2022-02-25 2022-10-06 주식회사 제이엘메디랩스 바이오마커 식별이 가능한 형광 현미경 및 이를 이용한 바이오마커 식별방법
WO2023191586A1 (ko) * 2022-03-31 2023-10-05 주식회사 제이엘메디랩스 위양성 신호가 제거된 항체 어레이 키트 및 이를 이용한 항원 검출방법
WO2023191539A1 (ko) * 2022-03-30 2023-10-05 주식회사 제이엘메디랩스 위양성 신호가 제거된 항원 검출방법 및 키트

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020179040A1 (ja) * 2019-03-06 2020-09-10 株式会社ニコン 顕微鏡および観察方法
CN110132910B (zh) * 2019-04-01 2022-01-28 中国科学院上海光学精密机械研究所 基于光场多维信息融合显微超分辨成像装置和成像方法
EP4318071A1 (en) * 2021-03-30 2024-02-07 Nikon Corporation Microscope

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JP3093009B2 (ja) * 1991-11-29 2000-10-03 オリンパス光学工業株式会社 落射蛍光顕微鏡
US5863504A (en) * 1995-03-16 1999-01-26 Bio-Rad Laboratories, Inc. Fluorescence imaging instrument utilizing fish
US5852498A (en) * 1997-04-04 1998-12-22 Kairos Scientific Inc. Optical instrument having a variable optical filter
DE19835070B4 (de) * 1998-08-04 2006-03-16 Carl Zeiss Jena Gmbh Anordnung zur einstellbaren wellenlängenabhängigen Detektion in einem Fluoreszenzmikroskop
JP2000098244A (ja) * 1998-09-24 2000-04-07 Olympus Optical Co Ltd 蛍光顕微鏡
DE19858206C2 (de) * 1998-12-17 2001-10-11 Leica Microsystems Verfahren zur Anpassung von Anregungsintensitäten bei einem Multiband-Fluoreszenz-Mikroskop und Multiband-Fluoreszenz-Mikroskop zur Durchführung des Verfahrens
US6654119B1 (en) * 1999-04-21 2003-11-25 Chromagen, Inc. Scanning spectrophotometer for high throughput fluroescence detection
DE10156695B4 (de) * 2001-11-17 2004-07-15 Leica Microsystems Heidelberg Gmbh Scanmikroskop, Verfahren zur Scanmikroskopie und Bandpassfilter
DE10227111B4 (de) * 2002-06-17 2007-09-27 Leica Microsystems Cms Gmbh Spektralmikroskop und Verfahren zur Datenaufnahme mit einem Spektralmikroskop
FR2842407B1 (fr) * 2002-07-18 2005-05-06 Mauna Kea Technologies "procede et appareillage d'imagerie de fluorescence confocale fibree"
DE10339311B4 (de) * 2003-08-27 2006-04-27 Leica Microsystems Cms Gmbh System und Verfahren zur Einstellung eines Fluoreszenzspektralmesssystems zur Mikroskopie
JP4804727B2 (ja) * 2004-06-24 2011-11-02 オリンパス株式会社 光走査型共焦点顕微鏡
JP4425098B2 (ja) * 2004-09-06 2010-03-03 浜松ホトニクス株式会社 蛍光顕微鏡および蛍光相関分光解析装置
US8189191B2 (en) * 2005-07-26 2012-05-29 Tufts University Spectroscopic imaging microscopy
JP2008299207A (ja) * 2007-06-01 2008-12-11 Nikon Corp 可変フィルタ装置及びコンフォーカル顕微鏡
AU2008320236A1 (en) * 2007-10-31 2009-05-07 Nikon Corporation Laser-exciting fluorescence microscope
JP5248924B2 (ja) * 2008-06-04 2013-07-31 オリンパス株式会社 レーザー走査型顕微鏡
JP2011186182A (ja) * 2010-03-09 2011-09-22 Claro Inc 顕微鏡装置
JP5771422B2 (ja) * 2010-06-17 2015-08-26 オリンパス株式会社 顕微鏡
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JP5549740B2 (ja) * 2010-10-14 2014-07-16 株式会社ニコン 構造化照明装置、構造化照明顕微鏡装置、及び面形状測定装置
CN102004307B (zh) * 2010-10-20 2012-06-27 中国科学院西安光学精密机械研究所 使用同心双锥面镜实现全内反射荧光显微的系统与方法
CN102998789B (zh) * 2012-12-31 2015-04-01 华中科技大学 一种超分辨微分干涉相衬显微成像方法
US20170016814A1 (en) * 2013-12-27 2017-01-19 Hitachi, Ltd. Biomolecule analysis device
CN104267015B (zh) * 2014-07-09 2017-01-18 香港应用科技研究院有限公司 用于生理检测的光信号检测装置及分析样本成分的方法
US10036880B2 (en) * 2014-11-07 2018-07-31 Olympus Corporation Microscope apparatus

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102450912B1 (ko) * 2022-02-25 2022-10-06 주식회사 제이엘메디랩스 바이오마커 식별이 가능한 형광 현미경 및 이를 이용한 바이오마커 식별방법
WO2023191539A1 (ko) * 2022-03-30 2023-10-05 주식회사 제이엘메디랩스 위양성 신호가 제거된 항원 검출방법 및 키트
WO2023191586A1 (ko) * 2022-03-31 2023-10-05 주식회사 제이엘메디랩스 위양성 신호가 제거된 항체 어레이 키트 및 이를 이용한 항원 검출방법

Also Published As

Publication number Publication date
US20190101738A1 (en) 2019-04-04
CN109313326B (zh) 2021-09-17
CN109313326A (zh) 2019-02-05
JPWO2017199407A1 (ja) 2019-03-07
US10914932B2 (en) 2021-02-09
WO2017199407A1 (ja) 2017-11-23

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