JP6625971B2 - 配列操作のためのタンデムガイド系、方法および組成物の送達、エンジニアリングおよび最適化 - Google Patents

配列操作のためのタンデムガイド系、方法および組成物の送達、エンジニアリングおよび最適化 Download PDF

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JP6625971B2
JP6625971B2 JP2016521449A JP2016521449A JP6625971B2 JP 6625971 B2 JP6625971 B2 JP 6625971B2 JP 2016521449 A JP2016521449 A JP 2016521449A JP 2016521449 A JP2016521449 A JP 2016521449A JP 6625971 B2 JP6625971 B2 JP 6625971B2
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sequence
tracr
cas9
composition
target
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フェン チャン
フェン チャン
チェ ユ リン
チェ ユ リン
フェイ ラン
フェイ ラン
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Massachusetts Institute of Technology
Broad Institute Inc
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
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JP2016521449A 2013-06-17 2014-06-10 配列操作のためのタンデムガイド系、方法および組成物の送達、エンジニアリングおよび最適化 Active JP6625971B2 (ja)

Applications Claiming Priority (15)

Application Number Priority Date Filing Date Title
US201361836123P 2013-06-17 2013-06-17
US61/836,123 2013-06-17
US201361847537P 2013-07-17 2013-07-17
US61/847,537 2013-07-17
US201361862468P 2013-08-05 2013-08-05
US201361862355P 2013-08-05 2013-08-05
US61/862,355 2013-08-05
US61/862,468 2013-08-05
US201361871301P 2013-08-28 2013-08-28
US61/871,301 2013-08-28
US201361915407P 2013-12-12 2013-12-12
US61/915,407 2013-12-12
US201461979942P 2014-04-15 2014-04-15
US61/979,942 2014-04-15
PCT/US2014/041800 WO2014204724A1 (en) 2013-06-17 2014-06-10 Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation

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JP6625971B2 true JP6625971B2 (ja) 2019-12-25

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JP2019215361A Pending JP2020054354A (ja) 2013-06-17 2019-11-28 配列操作のためのタンデムガイド系、方法および組成物の送達、エンジニアリングおよび最適化

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US (2) US11008588B2 (cg-RX-API-DMAC7.html)
EP (2) EP3674411A1 (cg-RX-API-DMAC7.html)
JP (2) JP6625971B2 (cg-RX-API-DMAC7.html)
KR (1) KR20160044457A (cg-RX-API-DMAC7.html)
CN (1) CN106062197A (cg-RX-API-DMAC7.html)
AU (1) AU2014281026B2 (cg-RX-API-DMAC7.html)
CA (1) CA2915834A1 (cg-RX-API-DMAC7.html)
DK (1) DK3011029T3 (cg-RX-API-DMAC7.html)
ES (1) ES2777217T3 (cg-RX-API-DMAC7.html)
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US12447213B2 (en) 2016-10-07 2025-10-21 The Broad Institute, Inc. Modulation of novel immune checkpoint targets

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US20160186213A1 (en) 2016-06-30
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