JP6076349B2 - ストレプトコッカス・ピオゲネス由来のエンドグリコシダーゼ及びその使用方法 - Google Patents

ストレプトコッカス・ピオゲネス由来のエンドグリコシダーゼ及びその使用方法 Download PDF

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JP6076349B2
JP6076349B2 JP2014530196A JP2014530196A JP6076349B2 JP 6076349 B2 JP6076349 B2 JP 6076349B2 JP 2014530196 A JP2014530196 A JP 2014530196A JP 2014530196 A JP2014530196 A JP 2014530196A JP 6076349 B2 JP6076349 B2 JP 6076349B2
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endos49
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コリン、マティアス
オールホーン、マリア
シェーグレン、ヨナタン
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ジェノビス エービー
ジェノビス エービー
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/005Glycopeptides, glycoproteins
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01096Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (3.2.1.96)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911Bacteria
    • G01N33/56944Streptococcus
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854Immunoglobulins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/40Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41Glycosylation, sialylation, or fucosylation
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2469/00Immunoassays for the detection of microorganisms
    • G01N2469/20Detection of antibodies in sample from host which are directed against antigens from microorganisms

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  • Health & Medical Sciences (AREA)
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  • Chemical & Material Sciences (AREA)
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  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2014530196A 2011-09-13 2012-09-12 ストレプトコッカス・ピオゲネス由来のエンドグリコシダーゼ及びその使用方法 Active JP6076349B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB201115841A GB201115841D0 (en) 2011-09-13 2011-09-13 Protein and method
GB1115841.7 2011-09-13
PCT/EP2012/067841 WO2013037824A1 (en) 2011-09-13 2012-09-12 Endoglycosidase from streptococcus pyogenes and methods using it

Publications (3)

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JP2014531205A JP2014531205A (ja) 2014-11-27
JP2014531205A5 JP2014531205A5 (enExample) 2015-10-29
JP6076349B2 true JP6076349B2 (ja) 2017-02-08

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JP2014530196A Active JP6076349B2 (ja) 2011-09-13 2012-09-12 ストレプトコッカス・ピオゲネス由来のエンドグリコシダーゼ及びその使用方法

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US (1) US9493752B2 (enExample)
EP (1) EP2756077B1 (enExample)
JP (1) JP6076349B2 (enExample)
KR (1) KR102001427B1 (enExample)
CN (1) CN103946377B (enExample)
AU (1) AU2012307461B2 (enExample)
BR (1) BR112014005816A2 (enExample)
CA (1) CA2848230C (enExample)
DK (1) DK2756077T3 (enExample)
EA (1) EA028490B8 (enExample)
ES (1) ES2627334T3 (enExample)
GB (2) GB201115841D0 (enExample)
IL (1) IL231410A0 (enExample)
IN (1) IN2014CN02163A (enExample)
MX (1) MX347727B (enExample)
PL (1) PL2756077T3 (enExample)
PT (1) PT2756077T (enExample)
SG (1) SG11201400505YA (enExample)
WO (1) WO2013037824A1 (enExample)
ZA (1) ZA201401840B (enExample)

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DE60134494D1 (de) * 2000-12-21 2008-07-31 Id Biomedical Corp Antigene aus streptococcus pyogenes und zugehörige dna-fragmente
SI2911699T2 (sl) 2012-10-23 2026-01-30 Synaffix B.V. Modificirano protitelo, protitelo-konjugat in proces za njihovo pripravo
SI2991683T1 (sl) * 2013-05-02 2020-01-31 Glykos Finland Oy Konjugati glikoproteina ali glikana s toksično obremenitvijo
EP2935611B1 (en) 2013-10-14 2021-07-07 SynAffix B.V. Glycoengineered antibody, antibody-conjugate and methods for their preparation
EP3057618B1 (en) 2013-10-14 2022-12-14 SynAffix B.V. Glycoengineered antibody, antibody-conjugate and methods for their preparation
US10072096B2 (en) 2013-10-14 2018-09-11 Synaffix B.V. Modified glycoprotein, protein-conjugate and process for the preparation thereof
EP3058083B1 (en) 2013-10-14 2018-04-11 SynAffix B.V. Modified glycoprotein, protein-conjugate and process for the preparation thereof
GB201318490D0 (en) * 2013-10-18 2013-12-04 Genovis Ab Method
EP3097079B1 (en) 2014-01-24 2021-05-12 SynAffix B.V. Process for the cycloaddition of a hetero(aryl) 1,3-dipole compound with a (hetero)cycloalkyne
WO2016001485A1 (en) 2014-06-30 2016-01-07 Glykos Finland Oy Saccharide derivative of a toxic payload and antibody conjugates thereof
GB201413240D0 (en) 2014-07-25 2014-09-10 Hansa Medical Ab Method
KR20170030648A (ko) 2014-08-04 2017-03-17 시나픽스 비.브이. 베타-(1,4)-n-아세틸갈락토오스아미닐트랜스퍼라아제, 또는 이의 돌연변이체를 이용한 당 단백질의 변형 방법
GB201502306D0 (en) 2015-02-12 2015-04-01 Hansa Medical Ab Protein
GB201502305D0 (en) * 2015-02-12 2015-04-01 Hansa Medical Ab Protein
CN107810273B (zh) 2015-04-23 2022-09-27 西纳福克斯股份有限公司 用为或衍生自β-(1,4)-N-乙酰半乳糖胺转移酶的糖基转移酶修饰糖蛋白的方法
US11008601B2 (en) * 2016-01-15 2021-05-18 University Of Maryland Endo-S2 mutants as glycosynthases, method of making and use for glycoengineering of glycoproteins
PL3411389T3 (pl) * 2016-02-04 2021-10-25 Genovis Ab Nowe proteazy streptokokowe
TWI727884B (zh) * 2016-08-24 2021-05-11 醣基生醫股份有限公司 利用醣苷內切酶突變體重塑醣蛋白及其使用方法
KR102608876B1 (ko) 2017-05-26 2023-11-30 게노비스 에이비 글리칸 분석을 위한 효소
US20210228738A1 (en) 2017-07-17 2021-07-29 INSERM (Institut National de la Santé et de la Recherche Médicale) Compositions and methods for increasing or enhancing transduction of gene therapy vectors and for removing or reducing immunoglobulins
CA3102152A1 (en) 2018-06-19 2019-12-26 Glykos Biomedical Oy Conjugate
WO2020002765A1 (en) 2018-06-29 2020-01-02 Glykos Biomedical Oy Conjugates
WO2021123506A1 (en) 2019-12-18 2021-06-24 Glykos Biomedical Oy Stabile conjugate
CN111044731B (zh) * 2019-12-19 2021-05-07 北京科技大学 脉冲温育免疫反应分离富集多肽药物中肽类杂质的方法
JP2023510349A (ja) 2020-01-09 2023-03-13 メルサナ セラピューティクス インコーポレイテッド ペプチド含有リンカーとの部位特異的抗体-薬物コンジュゲート
GB202002072D0 (en) * 2020-02-14 2020-04-01 Hansa Biopharma AB immunoglobulin detection and associated therapies
CN115515631B (zh) * 2020-09-21 2024-12-24 上海宝济药业股份有限公司 一种药物组合及其应用
AU2022205057A1 (en) 2021-01-04 2023-07-13 Mersana Therapeutics, Inc. B7h4-targeted antibody-drug conjugates and methods of use thereof
EP4079848A1 (en) 2021-04-22 2022-10-26 Genovis Ab Immunoglobulin cleaving enzyme
CN115873833B (zh) * 2022-11-08 2024-08-16 上海泰昶生物技术有限公司 生产免疫球蛋白g降解酶的工程菌株和工艺
CN117165643B (zh) * 2023-08-17 2025-10-28 山东大学 一种糖苷内切酶在水解高甘露糖型n-糖蛋白中的应用
WO2025098484A1 (zh) * 2023-11-10 2025-05-15 上海齐鲁制药研究中心有限公司 新型Endo S2突变体酶
WO2025180451A1 (zh) * 2024-03-01 2025-09-04 上海齐鲁制药研究中心有限公司 新型Endo S2突变体酶
WO2025248500A1 (en) 2024-05-31 2025-12-04 Janssen Biotech, Inc. Antibody drug conjugates that target enpp3

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GB0130228D0 (en) 2001-12-18 2002-02-06 Hansa Medica Ab Protein
GB0624874D0 (en) * 2006-12-13 2007-01-24 Hansa Medical Ab Treatment
WO2009033670A2 (en) * 2007-09-14 2009-03-19 Genovis Ab Method and kits for dissociating fcgamma-receptor-igg complexes and for igg purification and detection
GB0821100D0 (en) 2008-11-18 2008-12-24 Hansa Medical Ab Antibodies
ES2528321T3 (es) * 2009-10-02 2015-02-06 Roche Glycart Ag Detección de a-fucosilación en anticuerpos

Also Published As

Publication number Publication date
CN103946377A (zh) 2014-07-23
ES2627334T3 (es) 2017-07-27
IL231410A0 (en) 2014-04-30
EA201490447A1 (ru) 2014-09-30
PT2756077T (pt) 2017-06-21
EP2756077A1 (en) 2014-07-23
AU2012307461B2 (en) 2016-08-18
MX2014002906A (es) 2014-10-17
EA028490B8 (ru) 2018-01-31
GB201115841D0 (en) 2011-10-26
SG11201400505YA (en) 2014-04-28
ZA201401840B (en) 2018-05-30
MX347727B (es) 2017-05-10
BR112014005816A2 (pt) 2020-12-29
CA2848230C (en) 2020-03-24
AU2012307461A1 (en) 2014-03-27
DK2756077T3 (en) 2017-07-03
JP2014531205A (ja) 2014-11-27
WO2013037824A1 (en) 2013-03-21
US20140302519A1 (en) 2014-10-09
KR102001427B1 (ko) 2019-07-18
GB2509284A (en) 2014-06-25
IN2014CN02163A (enExample) 2015-05-29
PL2756077T3 (pl) 2017-09-29
EP2756077B1 (en) 2017-03-29
EA028490B1 (ru) 2017-11-30
NZ622172A (en) 2016-04-29
GB201406321D0 (en) 2014-05-21
US9493752B2 (en) 2016-11-15
KR20140081813A (ko) 2014-07-01
CA2848230A1 (en) 2013-03-21
CN103946377B (zh) 2016-10-05

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