EA028490B8 - Способ дегликозилирования иммуноглобулина - Google Patents
Способ дегликозилирования иммуноглобулинаInfo
- Publication number
- EA028490B8 EA028490B8 EA201490447A EA201490447A EA028490B8 EA 028490 B8 EA028490 B8 EA 028490B8 EA 201490447 A EA201490447 A EA 201490447A EA 201490447 A EA201490447 A EA 201490447A EA 028490 B8 EA028490 B8 EA 028490B8
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- endos49
- igg
- mutant
- endos
- seq
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01096—Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (3.2.1.96)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/56944—Streptococcus
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
- C07K2317/41—Glycosylation, sialylation, or fucosylation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2469/00—Immunoassays for the detection of microorganisms
- G01N2469/20—Detection of antibodies in sample from host which are directed against antigens from microorganisms
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Согласно изобретению предложена эндогликозидаза, обозначаемая EndoS49 и имеющая аминокислотную последовательность SEQ ID NO: 1. EndoS49 выделяли из штамма NZ131 Streptococcus pyogenes, она представляет собой гомолог EndoS. EndoS49 обладает специфичной эндогликозидазной активностью в отношении нативного IgG и расщепляет более широкий спектр гликанов Fc, чем EndoS. Получен ее мутант с заменой глутаминовой кислоты в положении 186 SEQ ID NO: 1; указанный мутант не обладает эндогликозидазной активностью, но способен связываться с IgG. Раскрыты способы с применением EndoS49, ее делеций и указанного мутанта особенно для оценки гликозилирования IgG или выделения IgG.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB201115841A GB201115841D0 (en) | 2011-09-13 | 2011-09-13 | Protein and method |
| PCT/EP2012/067841 WO2013037824A1 (en) | 2011-09-13 | 2012-09-12 | Endoglycosidase from streptococcus pyogenes and methods using it |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| EA201490447A1 EA201490447A1 (ru) | 2014-09-30 |
| EA028490B1 EA028490B1 (ru) | 2017-11-30 |
| EA028490B8 true EA028490B8 (ru) | 2018-01-31 |
Family
ID=44908522
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA201490447A EA028490B8 (ru) | 2011-09-13 | 2012-09-12 | Способ дегликозилирования иммуноглобулина |
Country Status (20)
| Country | Link |
|---|---|
| US (1) | US9493752B2 (ru) |
| EP (1) | EP2756077B1 (ru) |
| JP (1) | JP6076349B2 (ru) |
| KR (1) | KR102001427B1 (ru) |
| CN (1) | CN103946377B (ru) |
| AU (1) | AU2012307461B2 (ru) |
| BR (1) | BR112014005816A2 (ru) |
| CA (1) | CA2848230C (ru) |
| DK (1) | DK2756077T3 (ru) |
| EA (1) | EA028490B8 (ru) |
| ES (1) | ES2627334T3 (ru) |
| GB (2) | GB201115841D0 (ru) |
| IL (1) | IL231410A0 (ru) |
| IN (1) | IN2014CN02163A (ru) |
| MX (1) | MX347727B (ru) |
| PL (1) | PL2756077T3 (ru) |
| PT (1) | PT2756077T (ru) |
| SG (1) | SG11201400505YA (ru) |
| WO (1) | WO2013037824A1 (ru) |
| ZA (1) | ZA201401840B (ru) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU1686302A (en) * | 2000-12-21 | 2002-07-01 | Shire Biochem Inc | Streptococcus pyogenes antigens and corresponding dna fragments |
| CN110201186A (zh) | 2012-10-23 | 2019-09-06 | 西纳福克斯股份有限公司 | 经修饰的抗体、抗体-缀合物及其制备方法 |
| DK2991683T3 (da) | 2013-05-02 | 2019-11-04 | Glykos Finland Oy | Konjugater af et glykoprotein eller en glykan med en toksisk ladning |
| US20160235861A1 (en) | 2013-10-14 | 2016-08-18 | SynAffix. B.V. | Glycoengineered antibody, antibody-conjugate and methods for their preparation |
| US10072096B2 (en) | 2013-10-14 | 2018-09-11 | Synaffix B.V. | Modified glycoprotein, protein-conjugate and process for the preparation thereof |
| US9987373B2 (en) | 2013-10-14 | 2018-06-05 | Synaffix B.V. | Modified glycoprotein, protein-conjugate and process for the preparation thereof |
| JP2016538878A (ja) | 2013-10-14 | 2016-12-15 | シンアフィックス ビー．ブイ． | 糖鎖工学的に操作された抗体、抗体コンジュゲート、及びそれらの調製方法 |
| GB201318490D0 (en) * | 2013-10-18 | 2013-12-04 | Genovis Ab | Method |
| US11168085B2 (en) | 2014-01-24 | 2021-11-09 | Synaffix B.V. | Process for the cycloaddition of a hetero(aryl) 1,3-dipole compound with a (hetero)cycloalkyne |
| AU2015282627B2 (en) | 2014-06-30 | 2020-04-02 | Glykos Finland Oy | Saccharide derivative of a toxic payload and antibody conjugates thereof |
| GB201413240D0 (en) | 2014-07-25 | 2014-09-10 | Hansa Medical Ab | Method |
| CN107109454A (zh) | 2014-08-04 | 2017-08-29 | 西纳福克斯股份有限公司 | 用β‑(1,4)‑N‑乙酰半乳糖胺转移酶或其突变体修饰糖蛋白的方法 |
| GB201502305D0 (en) * | 2015-02-12 | 2015-04-01 | Hansa Medical Ab | Protein |
| GB201502306D0 (en) | 2015-02-12 | 2015-04-01 | Hansa Medical Ab | Protein |
| DK3134520T3 (en) | 2015-04-23 | 2018-03-19 | Synaffix Bv | Process for modifying a glycoprotein using a glycosyltransferase which is or is derived from a (1,4) -N-acetylgalactosaminyltransferase |
| WO2017124084A1 (en) * | 2016-01-15 | 2017-07-20 | University Of Maryland, College Park | Endo-s2 mutants as glycosynthases, method of making and use for glycoengineering of glycoproteins |
| PL3411389T3 (pl) * | 2016-02-04 | 2021-10-25 | Genovis Ab | Nowe proteazy streptokokowe |
| TWI727884B (zh) * | 2016-08-24 | 2021-05-11 | 醣基生醫股份有限公司 | 利用醣苷內切酶突變體重塑醣蛋白及其使用方法 |
| JP7195008B2 (ja) | 2017-05-26 | 2022-12-23 | ジェノビス エービー | グリカン分析のための酵素 |
| US20210228738A1 (en) | 2017-07-17 | 2021-07-29 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Compositions and methods for increasing or enhancing transduction of gene therapy vectors and for removing or reducing immunoglobulins |
| CA3102152A1 (en) | 2018-06-19 | 2019-12-26 | Glykos Biomedical Oy | Conjugate |
| CN112672765A (zh) | 2018-06-29 | 2021-04-16 | 格莱科斯生物医药公司 | 结合物 |
| US20230038373A1 (en) | 2019-12-18 | 2023-02-09 | Glykos Biomedical Oy | Stabile conjugate |
| CN111044731B (zh) * | 2019-12-19 | 2021-05-07 | 北京科技大学 | 脉冲温育免疫反应分离富集多肽药物中肽类杂质的方法 |
| US11786605B2 (en) | 2020-01-09 | 2023-10-17 | Mersana Therapeutics, Inc. | Site specific antibody-drug conjugates with peptide-containing linkers |
| EP4215208A1 (en) * | 2020-09-21 | 2023-07-26 | Shanghai Bao Pharmaceuticals Co., Ltd. | Pharmaceutical combination and use thereof |
| KR20230137339A (ko) | 2021-01-04 | 2023-10-04 | 메르사나 테라퓨틱스, 인코포레이티드 | B7h4-표적화된 항체-약물 접합체 및 이의 사용 방법 |
| EP4079848A1 (en) | 2021-04-22 | 2022-10-26 | Genovis Ab | Immunoglobulin cleaving enzyme |
| CN115873833A (zh) * | 2022-11-08 | 2023-03-31 | 上海泰昶生物技术有限公司 | 生产免疫球蛋白g降解酶的工程菌株和工艺 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0130228D0 (en) | 2001-12-18 | 2002-02-06 | Hansa Medica Ab | Protein |
| GB0624874D0 (en) * | 2006-12-13 | 2007-01-24 | Hansa Medical Ab | Treatment |
| US8323908B2 (en) * | 2007-09-14 | 2012-12-04 | Genovis Ab | Method of assessing glycosylation state or functional quality of an IgG containing sample |
| GB0821100D0 (en) | 2008-11-18 | 2008-12-24 | Hansa Medical Ab | Antibodies |
| WO2011039150A1 (en) * | 2009-10-02 | 2011-04-07 | Roche Glycart Ag | A-fucosylation detection in antibodies |
-
2011
- 2011-09-13 GB GB201115841A patent/GB201115841D0/en not_active Ceased
-
2012
- 2012-09-12 PL PL12756523T patent/PL2756077T3/pl unknown
- 2012-09-12 AU AU2012307461A patent/AU2012307461B2/en active Active
- 2012-09-12 IN IN2163CHN2014 patent/IN2014CN02163A/en unknown
- 2012-09-12 JP JP2014530196A patent/JP6076349B2/ja active Active
- 2012-09-12 GB GB1406321.8A patent/GB2509284A/en not_active Withdrawn
- 2012-09-12 EP EP12756523.2A patent/EP2756077B1/en active Active
- 2012-09-12 SG SG11201400505YA patent/SG11201400505YA/en unknown
- 2012-09-12 WO PCT/EP2012/067841 patent/WO2013037824A1/en active Application Filing
- 2012-09-12 US US14/343,938 patent/US9493752B2/en active Active
- 2012-09-12 CA CA2848230A patent/CA2848230C/en active Active
- 2012-09-12 MX MX2014002906A patent/MX347727B/es active IP Right Grant
- 2012-09-12 DK DK12756523.2T patent/DK2756077T3/en active
- 2012-09-12 KR KR1020147009205A patent/KR102001427B1/ko active IP Right Grant
- 2012-09-12 EA EA201490447A patent/EA028490B8/ru not_active IP Right Cessation
- 2012-09-12 ES ES12756523.2T patent/ES2627334T3/es active Active
- 2012-09-12 PT PT127565232T patent/PT2756077T/pt unknown
- 2012-09-12 BR BR112014005816-4A patent/BR112014005816A2/pt not_active IP Right Cessation
- 2012-09-12 CN CN201280044106.0A patent/CN103946377B/zh active Active
-
2014
- 2014-03-09 IL IL231410A patent/IL231410A0/en unknown
- 2014-03-12 ZA ZA2014/01840A patent/ZA201401840B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| KR102001427B1 (ko) | 2019-07-18 |
| WO2013037824A1 (en) | 2013-03-21 |
| JP2014531205A (ja) | 2014-11-27 |
| GB2509284A (en) | 2014-06-25 |
| JP6076349B2 (ja) | 2017-02-08 |
| CA2848230A1 (en) | 2013-03-21 |
| AU2012307461B2 (en) | 2016-08-18 |
| EA201490447A1 (ru) | 2014-09-30 |
| PT2756077T (pt) | 2017-06-21 |
| ZA201401840B (en) | 2018-05-30 |
| CN103946377A (zh) | 2014-07-23 |
| BR112014005816A2 (pt) | 2020-12-29 |
| CN103946377B (zh) | 2016-10-05 |
| GB201406321D0 (en) | 2014-05-21 |
| IN2014CN02163A (ru) | 2015-05-29 |
| US20140302519A1 (en) | 2014-10-09 |
| MX2014002906A (es) | 2014-10-17 |
| PL2756077T3 (pl) | 2017-09-29 |
| EP2756077A1 (en) | 2014-07-23 |
| KR20140081813A (ko) | 2014-07-01 |
| US9493752B2 (en) | 2016-11-15 |
| SG11201400505YA (en) | 2014-04-28 |
| ES2627334T3 (es) | 2017-07-27 |
| AU2012307461A1 (en) | 2014-03-27 |
| IL231410A0 (en) | 2014-04-30 |
| DK2756077T3 (en) | 2017-07-03 |
| EP2756077B1 (en) | 2017-03-29 |
| GB201115841D0 (en) | 2011-10-26 |
| CA2848230C (en) | 2020-03-24 |
| EA028490B1 (ru) | 2017-11-30 |
| NZ622172A (en) | 2016-04-29 |
| MX347727B (es) | 2017-05-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): AM AZ BY KZ KG TJ TM RU |