MX357484B - Cepa con deficit de amilasa. - Google Patents

Cepa con deficit de amilasa.

Info

Publication number
MX357484B
MX357484B MX2015000844A MX2015000844A MX357484B MX 357484 B MX357484 B MX 357484B MX 2015000844 A MX2015000844 A MX 2015000844A MX 2015000844 A MX2015000844 A MX 2015000844A MX 357484 B MX357484 B MX 357484B
Authority
MX
Mexico
Prior art keywords
amylase
deficient strain
host cell
microbial host
mutant microbial
Prior art date
Application number
MX2015000844A
Other languages
English (en)
Other versions
MX2015000844A (es
Inventor
Nicolaas Maria Elisabeth Van Peij Noël
Beishuizen Martina
Jozef Ida Van De Vondervoort Peter
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Publication of MX2015000844A publication Critical patent/MX2015000844A/es
Publication of MX357484B publication Critical patent/MX357484B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242—Fungal source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
    • C12Y101/03004—Glucose oxidase (1.1.3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente invención se refiere a una célula hospedadora microbiana mutante que se ha modificado, preferiblemente en su genoma, para dar como resultado un déficit en la producción de un polipéptido que tiene actividad a-amilasa AmiC o un homólogo del mismo. Sorprendentemente se ha descubierto que cuando la cepa hospedadora microbiana mutante según la invención se usa en un método para producir un compuesto de interés, por ejemplo, una enzima, se obtiene una pureza mejorada del producto.
MX2015000844A 2012-07-19 2013-07-19 Cepa con deficit de amilasa. MX357484B (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261673607P 2012-07-19 2012-07-19
US201261673589P 2012-07-19 2012-07-19
EP12177171 2012-07-19
EP12177173 2012-07-19
PCT/EP2013/065347 WO2014013073A1 (en) 2012-07-19 2013-07-19 Amylase-deficient strain

Publications (2)

Publication Number Publication Date
MX2015000844A MX2015000844A (es) 2015-04-08
MX357484B true MX357484B (es) 2018-07-11

Family

ID=49948319

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015000844A MX357484B (es) 2012-07-19 2013-07-19 Cepa con deficit de amilasa.

Country Status (5)

Country Link
US (1) US9745563B2 (es)
EP (1) EP2875120B1 (es)
CN (1) CN105189730B (es)
MX (1) MX357484B (es)
WO (1) WO2014013073A1 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015082488A1 (en) 2013-12-02 2015-06-11 Dsm Ip Assets B.V. Ice structuring protein
US11396665B2 (en) 2015-01-06 2022-07-26 Dsm Ip Assets B.V. CRISPR-CAS system for a filamentous fungal host cell
WO2016193292A1 (en) 2015-06-02 2016-12-08 Dsm Ip Assets B.V. Use of ice structuring protein afp19 expressed in filamentous fungal strains for preparing food
CN106591261A (zh) * 2015-10-14 2017-04-26 中国科学院天津工业生物技术研究所 一种α淀粉酶及高产α淀粉酶的诱变菌株
WO2018019948A1 (en) 2016-07-29 2018-02-01 Dsm Ip Assets B.V. Polypeptides having cellulolytic enhancing activity and uses thereof
WO2018114940A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
US10918113B2 (en) 2016-12-21 2021-02-16 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114912A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114938A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
CN110431224A (zh) 2017-03-13 2019-11-08 帝斯曼知识产权资产管理有限公司 锌双核簇转录调控因子缺陷型菌株
US12195769B2 (en) 2018-06-19 2025-01-14 Dsm Ip Assets B.V. Lipolytic enzyme variants
CN110791439B (zh) * 2019-10-10 2022-04-19 天津科技大学 一株基因工程构建发酵生产苹果酸的重组黑曲霉菌株及应用
US20230212634A1 (en) * 2020-01-14 2023-07-06 The Protein Brewery B.V. Expression of Ovalbumin and its Natural Variants
EP4359426A2 (en) 2021-06-24 2024-05-01 Fonterra Co-Operative Group Limited Recombinant beta-lactoglobulin
CN114456952B (zh) * 2022-02-22 2023-04-14 昆明理工大学 一种金孢菌及其应用
US20250311734A1 (en) 2022-05-14 2025-10-09 Novozymes A/S Compositions and Methods for Preventing, Treating, Suppressing and/or Eliminatting Phytopathogenic Infestations and Infections
WO2025012325A1 (en) 2023-07-11 2025-01-16 Dsm Ip Assets B.V. Protein arginine deiminase

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2703598B2 (ja) 1987-09-04 1998-01-26 ノボ―ノルディスク アクティーゼルスカブ アスペルギルス菌中でのタンパク質生産物の生産方法およびアスペルギルス菌中で使用するためのプロモーター
JP2005500063A (ja) * 2001-08-16 2005-01-06 デーエスエム イーペー アセッツ ベスローテン フェンノートシャップ 新規なアミラーゼおよびその使用
EP2421986A1 (en) * 2009-04-22 2012-02-29 DSM IP Assets B.V. Process for the production of a recombinant polypeptide of interest
EP3293264A1 (en) * 2009-07-22 2018-03-14 DSM IP Assets B.V. Improved host cell for the production of a compound of interest
CA2803222A1 (en) 2010-07-01 2012-01-05 Dsm Ip Assets B.V. A method for the production of a compound of interest

Also Published As

Publication number Publication date
WO2014013073A1 (en) 2014-01-23
EP2875120B1 (en) 2019-03-20
US9745563B2 (en) 2017-08-29
MX2015000844A (es) 2015-04-08
EP2875120A1 (en) 2015-05-27
CN105189730B (zh) 2021-04-30
US20150166970A1 (en) 2015-06-18
CN105189730A (zh) 2015-12-23

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