MX347727B - Endoglicosidasa del streptococcus pyogenes y metodos para usarla. - Google Patents
Endoglicosidasa del streptococcus pyogenes y metodos para usarla.Info
- Publication number
- MX347727B MX347727B MX2014002906A MX2014002906A MX347727B MX 347727 B MX347727 B MX 347727B MX 2014002906 A MX2014002906 A MX 2014002906A MX 2014002906 A MX2014002906 A MX 2014002906A MX 347727 B MX347727 B MX 347727B
- Authority
- MX
- Mexico
- Prior art keywords
- endos49
- igg
- endoglycosidase
- mutant
- methods
- Prior art date
Links
- 102000005744 Glycoside Hydrolases Human genes 0.000 title abstract 3
- 108010031186 Glycoside Hydrolases Proteins 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 241000193996 Streptococcus pyogenes Species 0.000 title 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 abstract 1
- 241001496716 Streptococcus pyogenes NZ131 Species 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 238000012217 deletion Methods 0.000 abstract 1
- 230000037430 deletion Effects 0.000 abstract 1
- 235000013922 glutamic acid Nutrition 0.000 abstract 1
- 239000004220 glutamic acid Substances 0.000 abstract 1
- 125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 abstract 1
- 230000013595 glycosylation Effects 0.000 abstract 1
- 238000006206 glycosylation reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01096—Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (3.2.1.96)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/56944—Streptococcus
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
- C07K2317/41—Glycosylation, sialylation, or fucosylation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2469/00—Immunoassays for the detection of microorganisms
- G01N2469/20—Detection of antibodies in sample from host which are directed against antigens from microorganisms
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
La presente invención se refiere a una endoglicosidasa, referida como EndoS49 y que tiene la secuencia de aminoácidos de SEQ ID NO: 1. La EndoS49 se aisló de la cepa NZ131 de Streptococcus pyogenes y es un homólogo de EndoS. La EndoS49 tiene actividad de endoglicosidasa sobre el IgG nativo y segmenta una variedad más grande de glicanos de Fc que la EndoS. Se produjo un mutante de la misma donde el ácido glutámico en la posición 186 de la SEQ ID NO: 1 se sustituyó: el mutante carece de actividad de endoglicosidasa pero es capaz de enlazar a IgG. Se describen métodos que utilizan EndoS49, supresiones de la misma y el mutante, especialmente para estimar la glucosilación de IgG o para aislar IgG.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB201115841A GB201115841D0 (en) | 2011-09-13 | 2011-09-13 | Protein and method |
| PCT/EP2012/067841 WO2013037824A1 (en) | 2011-09-13 | 2012-09-12 | Endoglycosidase from streptococcus pyogenes and methods using it |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2014002906A MX2014002906A (es) | 2014-10-17 |
| MX347727B true MX347727B (es) | 2017-05-10 |
Family
ID=44908522
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2014002906A MX347727B (es) | 2011-09-13 | 2012-09-12 | Endoglicosidasa del streptococcus pyogenes y metodos para usarla. |
Country Status (20)
| Country | Link |
|---|---|
| US (1) | US9493752B2 (es) |
| EP (1) | EP2756077B1 (es) |
| JP (1) | JP6076349B2 (es) |
| KR (1) | KR102001427B1 (es) |
| CN (1) | CN103946377B (es) |
| AU (1) | AU2012307461B2 (es) |
| BR (1) | BR112014005816A2 (es) |
| CA (1) | CA2848230C (es) |
| DK (1) | DK2756077T3 (es) |
| EA (1) | EA028490B8 (es) |
| ES (1) | ES2627334T3 (es) |
| GB (2) | GB201115841D0 (es) |
| IL (1) | IL231410A0 (es) |
| IN (1) | IN2014CN02163A (es) |
| MX (1) | MX347727B (es) |
| PL (1) | PL2756077T3 (es) |
| PT (1) | PT2756077T (es) |
| SG (1) | SG11201400505YA (es) |
| WO (1) | WO2013037824A1 (es) |
| ZA (1) | ZA201401840B (es) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE60134494D1 (de) * | 2000-12-21 | 2008-07-31 | Id Biomedical Corp | Antigene aus streptococcus pyogenes und zugehörige dna-fragmente |
| SI2911699T2 (sl) | 2012-10-23 | 2026-01-30 | Synaffix B.V. | Modificirano protitelo, protitelo-konjugat in proces za njihovo pripravo |
| SI2991683T1 (sl) * | 2013-05-02 | 2020-01-31 | Glykos Finland Oy | Konjugati glikoproteina ali glikana s toksično obremenitvijo |
| EP2935611B1 (en) | 2013-10-14 | 2021-07-07 | SynAffix B.V. | Glycoengineered antibody, antibody-conjugate and methods for their preparation |
| EP3057618B1 (en) | 2013-10-14 | 2022-12-14 | SynAffix B.V. | Glycoengineered antibody, antibody-conjugate and methods for their preparation |
| US10072096B2 (en) | 2013-10-14 | 2018-09-11 | Synaffix B.V. | Modified glycoprotein, protein-conjugate and process for the preparation thereof |
| EP3058083B1 (en) | 2013-10-14 | 2018-04-11 | SynAffix B.V. | Modified glycoprotein, protein-conjugate and process for the preparation thereof |
| GB201318490D0 (en) * | 2013-10-18 | 2013-12-04 | Genovis Ab | Method |
| EP3097079B1 (en) | 2014-01-24 | 2021-05-12 | SynAffix B.V. | Process for the cycloaddition of a hetero(aryl) 1,3-dipole compound with a (hetero)cycloalkyne |
| WO2016001485A1 (en) | 2014-06-30 | 2016-01-07 | Glykos Finland Oy | Saccharide derivative of a toxic payload and antibody conjugates thereof |
| GB201413240D0 (en) | 2014-07-25 | 2014-09-10 | Hansa Medical Ab | Method |
| KR20170030648A (ko) | 2014-08-04 | 2017-03-17 | 시나픽스 비.브이. | 베타-(1,4)-n-아세틸갈락토오스아미닐트랜스퍼라아제, 또는 이의 돌연변이체를 이용한 당 단백질의 변형 방법 |
| GB201502306D0 (en) | 2015-02-12 | 2015-04-01 | Hansa Medical Ab | Protein |
| GB201502305D0 (en) * | 2015-02-12 | 2015-04-01 | Hansa Medical Ab | Protein |
| CN107810273B (zh) | 2015-04-23 | 2022-09-27 | 西纳福克斯股份有限公司 | 用为或衍生自β-(1,4)-N-乙酰半乳糖胺转移酶的糖基转移酶修饰糖蛋白的方法 |
| US11008601B2 (en) * | 2016-01-15 | 2021-05-18 | University Of Maryland | Endo-S2 mutants as glycosynthases, method of making and use for glycoengineering of glycoproteins |
| PL3411389T3 (pl) * | 2016-02-04 | 2021-10-25 | Genovis Ab | Nowe proteazy streptokokowe |
| TWI727884B (zh) * | 2016-08-24 | 2021-05-11 | 醣基生醫股份有限公司 | 利用醣苷內切酶突變體重塑醣蛋白及其使用方法 |
| KR102608876B1 (ko) | 2017-05-26 | 2023-11-30 | 게노비스 에이비 | 글리칸 분석을 위한 효소 |
| US20210228738A1 (en) | 2017-07-17 | 2021-07-29 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Compositions and methods for increasing or enhancing transduction of gene therapy vectors and for removing or reducing immunoglobulins |
| CA3102152A1 (en) | 2018-06-19 | 2019-12-26 | Glykos Biomedical Oy | Conjugate |
| WO2020002765A1 (en) | 2018-06-29 | 2020-01-02 | Glykos Biomedical Oy | Conjugates |
| WO2021123506A1 (en) | 2019-12-18 | 2021-06-24 | Glykos Biomedical Oy | Stabile conjugate |
| CN111044731B (zh) * | 2019-12-19 | 2021-05-07 | 北京科技大学 | 脉冲温育免疫反应分离富集多肽药物中肽类杂质的方法 |
| JP2023510349A (ja) | 2020-01-09 | 2023-03-13 | メルサナ セラピューティクス インコーポレイテッド | ペプチド含有リンカーとの部位特異的抗体－薬物コンジュゲート |
| GB202002072D0 (en) * | 2020-02-14 | 2020-04-01 | Hansa Biopharma AB | immunoglobulin detection and associated therapies |
| CN115515631B (zh) * | 2020-09-21 | 2024-12-24 | 上海宝济药业股份有限公司 | 一种药物组合及其应用 |
| AU2022205057A1 (en) | 2021-01-04 | 2023-07-13 | Mersana Therapeutics, Inc. | B7h4-targeted antibody-drug conjugates and methods of use thereof |
| EP4079848A1 (en) | 2021-04-22 | 2022-10-26 | Genovis Ab | Immunoglobulin cleaving enzyme |
| CN115873833B (zh) * | 2022-11-08 | 2024-08-16 | 上海泰昶生物技术有限公司 | 生产免疫球蛋白g降解酶的工程菌株和工艺 |
| CN117165643B (zh) * | 2023-08-17 | 2025-10-28 | 山东大学 | 一种糖苷内切酶在水解高甘露糖型n-糖蛋白中的应用 |
| WO2025098484A1 (zh) * | 2023-11-10 | 2025-05-15 | 上海齐鲁制药研究中心有限公司 | 新型Endo S2突变体酶 |
| WO2025180451A1 (zh) * | 2024-03-01 | 2025-09-04 | 上海齐鲁制药研究中心有限公司 | 新型Endo S2突变体酶 |
| WO2025248500A1 (en) | 2024-05-31 | 2025-12-04 | Janssen Biotech, Inc. | Antibody drug conjugates that target enpp3 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0130228D0 (en) | 2001-12-18 | 2002-02-06 | Hansa Medica Ab | Protein |
| GB0624874D0 (en) * | 2006-12-13 | 2007-01-24 | Hansa Medical Ab | Treatment |
| WO2009033670A2 (en) * | 2007-09-14 | 2009-03-19 | Genovis Ab | Method and kits for dissociating fcgamma-receptor-igg complexes and for igg purification and detection |
| GB0821100D0 (en) | 2008-11-18 | 2008-12-24 | Hansa Medical Ab | Antibodies |
| ES2528321T3 (es) * | 2009-10-02 | 2015-02-06 | Roche Glycart Ag | Detección de a-fucosilación en anticuerpos |
-
2011
- 2011-09-13 GB GB201115841A patent/GB201115841D0/en not_active Ceased
-
2012
- 2012-09-12 EA EA201490447A patent/EA028490B8/ru not_active IP Right Cessation
- 2012-09-12 JP JP2014530196A patent/JP6076349B2/ja active Active
- 2012-09-12 AU AU2012307461A patent/AU2012307461B2/en active Active
- 2012-09-12 ES ES12756523.2T patent/ES2627334T3/es active Active
- 2012-09-12 MX MX2014002906A patent/MX347727B/es active IP Right Grant
- 2012-09-12 PT PT127565232T patent/PT2756077T/pt unknown
- 2012-09-12 CA CA2848230A patent/CA2848230C/en active Active
- 2012-09-12 EP EP12756523.2A patent/EP2756077B1/en active Active
- 2012-09-12 SG SG11201400505YA patent/SG11201400505YA/en unknown
- 2012-09-12 DK DK12756523.2T patent/DK2756077T3/en active
- 2012-09-12 WO PCT/EP2012/067841 patent/WO2013037824A1/en not_active Ceased
- 2012-09-12 IN IN2163CHN2014 patent/IN2014CN02163A/en unknown
- 2012-09-12 GB GB1406321.8A patent/GB2509284A/en not_active Withdrawn
- 2012-09-12 PL PL12756523T patent/PL2756077T3/pl unknown
- 2012-09-12 BR BR112014005816-4A patent/BR112014005816A2/pt not_active IP Right Cessation
- 2012-09-12 CN CN201280044106.0A patent/CN103946377B/zh active Active
- 2012-09-12 KR KR1020147009205A patent/KR102001427B1/ko active Active
- 2012-09-12 US US14/343,938 patent/US9493752B2/en active Active
-
2014
- 2014-03-09 IL IL231410A patent/IL231410A0/en unknown
- 2014-03-12 ZA ZA2014/01840A patent/ZA201401840B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CN103946377A (zh) | 2014-07-23 |
| ES2627334T3 (es) | 2017-07-27 |
| IL231410A0 (en) | 2014-04-30 |
| JP6076349B2 (ja) | 2017-02-08 |
| EA201490447A1 (ru) | 2014-09-30 |
| PT2756077T (pt) | 2017-06-21 |
| EP2756077A1 (en) | 2014-07-23 |
| AU2012307461B2 (en) | 2016-08-18 |
| MX2014002906A (es) | 2014-10-17 |
| EA028490B8 (ru) | 2018-01-31 |
| GB201115841D0 (en) | 2011-10-26 |
| SG11201400505YA (en) | 2014-04-28 |
| ZA201401840B (en) | 2018-05-30 |
| BR112014005816A2 (pt) | 2020-12-29 |
| CA2848230C (en) | 2020-03-24 |
| AU2012307461A1 (en) | 2014-03-27 |
| DK2756077T3 (en) | 2017-07-03 |
| JP2014531205A (ja) | 2014-11-27 |
| WO2013037824A1 (en) | 2013-03-21 |
| US20140302519A1 (en) | 2014-10-09 |
| KR102001427B1 (ko) | 2019-07-18 |
| GB2509284A (en) | 2014-06-25 |
| IN2014CN02163A (es) | 2015-05-29 |
| PL2756077T3 (pl) | 2017-09-29 |
| EP2756077B1 (en) | 2017-03-29 |
| EA028490B1 (ru) | 2017-11-30 |
| NZ622172A (en) | 2016-04-29 |
| GB201406321D0 (en) | 2014-05-21 |
| US9493752B2 (en) | 2016-11-15 |
| KR20140081813A (ko) | 2014-07-01 |
| CA2848230A1 (en) | 2013-03-21 |
| CN103946377B (zh) | 2016-10-05 |
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