ES2528321T3 - Detección de a-fucosilación en anticuerpos - Google Patents
Detección de a-fucosilación en anticuerpos Download PDFInfo
- Publication number
- ES2528321T3 ES2528321T3 ES10757221.6T ES10757221T ES2528321T3 ES 2528321 T3 ES2528321 T3 ES 2528321T3 ES 10757221 T ES10757221 T ES 10757221T ES 2528321 T3 ES2528321 T3 ES 2528321T3
- Authority
- ES
- Spain
- Prior art keywords
- protein
- fucosylation
- antibodies
- detection
- residues
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01096—Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (3.2.1.96)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/17—Metallocarboxypeptidases (3.4.17)
- C12Y304/17002—Carboxypeptidase B (3.4.17.2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21007—Plasmin (3.4.21.7), i.e. fibrinolysin
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
- C07K2317/41—Glycosylation, sialylation, or fucosylation
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/52—Constant or Fc region; Isotype
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/968—Plasmin, i.e. fibrinolysin
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
- G01N2400/02—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates involving antibodies to sugar part of glycoproteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
- G01N2440/38—Post-translational modifications [PTMs] in chemical analysis of biological material addition of carbohydrates, e.g. glycosylation, glycation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2560/00—Chemical aspects of mass spectrometric analysis of biological material
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Immunology (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Método para detectar la presencia o la ausencia de residuos de fucosa dentro de un anticuerpo glucosilado o de un fragmento del mismo, que comprende: a) eliminación enzimática de residuos sacáridos heterogéneos de la proteína, b) eliminación enzimática de otros residuos heterogéneos de la proteína, c) posterior análisis de la proteína, en el que la eliminación en la etapa a) se lleva a cabo con Endo S y Endo H.
Description
Claims (1)
-
imagen1
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP09172130 | 2009-10-02 | ||
EP09172130 | 2009-10-02 | ||
PCT/EP2010/064291 WO2011039150A1 (en) | 2009-10-02 | 2010-09-28 | A-fucosylation detection in antibodies |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2528321T3 true ES2528321T3 (es) | 2015-02-06 |
Family
ID=43037129
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES10757221.6T Active ES2528321T3 (es) | 2009-10-02 | 2010-09-28 | Detección de a-fucosilación en anticuerpos |
Country Status (8)
Country | Link |
---|---|
US (3) | US20120196310A1 (es) |
EP (1) | EP2483693B1 (es) |
JP (2) | JP2013506404A (es) |
CN (1) | CN102511007B (es) |
CA (1) | CA2773882C (es) |
ES (1) | ES2528321T3 (es) |
HK (1) | HK1168653A1 (es) |
WO (1) | WO2011039150A1 (es) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2560470T3 (es) * | 2011-04-29 | 2016-02-19 | Biocon Research Limited | Un método para reducir la heterogeneidad de anticuerpos y un proceso de producción de dichos anticuerpos |
GB201115841D0 (en) * | 2011-09-13 | 2011-10-26 | Genovis Ab | Protein and method |
EP2768845B1 (en) | 2011-10-19 | 2017-01-18 | Roche Glycart AG | Separation method for fucosylated antibodies |
CA2862925C (en) | 2012-02-10 | 2020-01-21 | University Of Maryland, Baltimore | Chemoenzymatic glycoengineering of antibodies and fc fragments thereof |
TWI682033B (zh) * | 2014-03-17 | 2020-01-11 | 泉盛生物科技股份有限公司 | 製造具有經修飾的糖苷化作用之重組糖蛋白之方法 |
JP6750148B2 (ja) * | 2014-04-25 | 2020-09-02 | 公益財団法人野口研究所 | 糖鎖切断抗体の製造方法及び均一糖鎖抗体 |
EP3064507A1 (en) | 2015-03-06 | 2016-09-07 | Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts | Fusion proteins comprising a binding protein and an interleukin-15 polypeptide having a reduced affinity for IL15ra and therapeutic uses thereof |
EP3414328B1 (en) | 2016-02-08 | 2024-01-10 | Synaffix B.V. | Enzymes for trimming of glycoproteins |
CN108982737B (zh) * | 2018-08-07 | 2021-06-22 | 中国人民解放军军事科学院军事医学研究院 | 一种规模化的核心岩藻糖基化修饰位点占有率定量方法 |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5126250A (en) * | 1988-09-28 | 1992-06-30 | Eli Lilly And Company | Method for the reduction of heterogeneity of monoclonal antibodies |
JP4253233B2 (ja) * | 2003-08-25 | 2009-04-08 | 大塚製薬株式会社 | 術後癌患者の予後判定方法 |
BRPI0607203A2 (pt) * | 2005-02-18 | 2009-08-25 | Medarex Inc | anticorpo anti-cd30 isolado, célula hospedeira, métodos para inibir o crescimento de células cd30+, e, uso de um anticorpo anti-cd30 desfucosilado |
CN101238148A (zh) * | 2005-06-03 | 2008-08-06 | 健泰科生物技术公司 | 具有改良的岩藻糖化水平的抗体的生产方法 |
US20070166306A1 (en) * | 2006-01-17 | 2007-07-19 | Fey Georg H M | Anti-CD19 antibody composition and method |
CN101460522B (zh) * | 2006-04-11 | 2014-07-23 | 弗·哈夫曼-拉罗切有限公司 | 糖基化抗体 |
US20080226635A1 (en) * | 2006-12-22 | 2008-09-18 | Hans Koll | Antibodies against insulin-like growth factor I receptor and uses thereof |
WO2008128228A1 (en) | 2007-04-16 | 2008-10-23 | Momenta Pharmaceuticals, Inc. | Methods related to cell surface glycosylation |
EP2190984B1 (en) | 2007-09-14 | 2013-09-04 | Genovis Ab | Methods and kits for purification and detection of glycosylated IgG |
-
2010
- 2010-09-28 CA CA2773882A patent/CA2773882C/en not_active Expired - Fee Related
- 2010-09-28 JP JP2012531345A patent/JP2013506404A/ja active Pending
- 2010-09-28 US US13/499,887 patent/US20120196310A1/en not_active Abandoned
- 2010-09-28 CN CN201080041521.1A patent/CN102511007B/zh active Active
- 2010-09-28 EP EP10757221.6A patent/EP2483693B1/en active Active
- 2010-09-28 WO PCT/EP2010/064291 patent/WO2011039150A1/en active Application Filing
- 2010-09-28 ES ES10757221.6T patent/ES2528321T3/es active Active
-
2012
- 2012-09-24 HK HK12109387.8A patent/HK1168653A1/xx unknown
-
2014
- 2014-05-22 US US14/285,440 patent/US20140302544A1/en not_active Abandoned
-
2015
- 2015-01-28 JP JP2015013936A patent/JP5980353B2/ja active Active
-
2016
- 2016-06-10 US US15/179,431 patent/US10352943B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
CA2773882C (en) | 2016-01-12 |
JP2015109857A (ja) | 2015-06-18 |
CA2773882A1 (en) | 2011-04-07 |
CN102511007B (zh) | 2015-04-15 |
CN102511007A (zh) | 2012-06-20 |
JP5980353B2 (ja) | 2016-08-31 |
US20140302544A1 (en) | 2014-10-09 |
US20170108510A1 (en) | 2017-04-20 |
WO2011039150A1 (en) | 2011-04-07 |
HK1168653A1 (en) | 2013-01-04 |
US20120196310A1 (en) | 2012-08-02 |
EP2483693A1 (en) | 2012-08-08 |
US10352943B2 (en) | 2019-07-16 |
JP2013506404A (ja) | 2013-02-28 |
EP2483693B1 (en) | 2014-12-03 |
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