MX340248B - Celobiohidrolasa mutante. - Google Patents

Celobiohidrolasa mutante.

Info

Publication number
MX340248B
MX340248B MX2013008766A MX2013008766A MX340248B MX 340248 B MX340248 B MX 340248B MX 2013008766 A MX2013008766 A MX 2013008766A MX 2013008766 A MX2013008766 A MX 2013008766A MX 340248 B MX340248 B MX 340248B
Authority
MX
Mexico
Prior art keywords
mutant
cellobiohydrolase
mutant cellobiohydrolase
seq
substitution
Prior art date
Application number
MX2013008766A
Other languages
English (en)
Other versions
MX2013008766A (es
Inventor
Metske Van Der Laan Jan
Elisabeth Francoise Schooneveld-Bergmans Margot
Ilse Jacobs Denise
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Publication of MX2013008766A publication Critical patent/MX2013008766A/es
Publication of MX340248B publication Critical patent/MX340248B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente invención se refiere a una celobiohidrolasa mutante, que es una mutante de la SEQ ID NO; 1, que comprende una sustitución en la posición N247(I, F,H,W) de la SEQ ID NO: 1, en donde dicha celobiohidrolasa mutante comprende una identidad de secuencia de al menos 50% con la SEQ ID NO: 1, y en donde dicha celobiohidrolasa mutante tiene actividad de celobiohidrolasa I (CBHI).
MX2013008766A 2011-01-31 2012-01-30 Celobiohidrolasa mutante. MX340248B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161437804P 2011-01-31 2011-01-31
EP11152691 2011-01-31
PCT/EP2012/051416 WO2012104239A1 (en) 2011-01-31 2012-01-30 Mutant cellobiohydrolase

Publications (2)

Publication Number Publication Date
MX2013008766A MX2013008766A (es) 2014-01-31
MX340248B true MX340248B (es) 2016-07-01

Family

ID=44140893

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013008766A MX340248B (es) 2011-01-31 2012-01-30 Celobiohidrolasa mutante.

Country Status (8)

Country Link
US (1) US8790894B2 (es)
EP (1) EP2670845A1 (es)
CN (1) CN103348005B (es)
BR (1) BR112013019514A2 (es)
CA (1) CA2824494A1 (es)
EA (1) EA023945B1 (es)
MX (1) MX340248B (es)
WO (1) WO2012104239A1 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112013019039A2 (pt) 2011-01-26 2020-07-21 Novozymes A/S Célula hospedeira microbiana transgênica, métodos para produzir um polipeptídeo, para produzir um mutante de uma célula originária, para produzir uma proteína, para degradar um material celulósico, para produzir um produto de fermentação, 5 e parafermentar um material celulósico, construção de ácido nucleico ou vetor de expressão, polipeptídeo isolado, e polinucleotídeo isolado
EP2668268B1 (en) 2011-01-26 2017-05-31 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
EP2931886A1 (en) * 2012-12-12 2015-10-21 Danisco US Inc. Variants of cellobiohydrolases
DK2964760T3 (da) 2013-03-08 2021-07-26 Novozymes Inc Cellobiohydrolasevarianter og polynukleotider, der koder for dem
JP6441330B2 (ja) 2014-05-27 2018-12-19 国立研究開発法人産業技術総合研究所 熱安定性が改善されたセロビオハイドロラーゼ
US11390898B2 (en) 2014-09-05 2022-07-19 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
EP3191587B1 (en) 2014-09-08 2019-07-17 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
JP6378622B2 (ja) 2014-12-04 2018-08-22 本田技研工業株式会社 高活性セロビオヒドロラーゼ
EP3739045B1 (en) 2015-02-24 2024-08-14 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
CN105176949B (zh) * 2015-10-21 2018-07-13 青岛蔚蓝生物集团有限公司 一种纤维二糖水解酶突变体
CN109337888B (zh) * 2018-11-12 2021-08-17 南开大学 纤维二糖水解酶突变体及其生产方法和应用
CN110923219B (zh) * 2019-12-27 2022-08-12 荣成市慧海创达生物科技有限公司 纤维二糖水解酶的突变体

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5811381A (en) * 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
US8637293B2 (en) * 1999-07-13 2014-01-28 Alliance For Sustainable Energy, Llc Cellobiohydrolase I enzymes
WO2004016760A2 (en) * 2002-08-16 2004-02-26 Genencor International, Inc. Novel variant hyprocrea jecorina cbh1 cellulases
ATE440504T1 (de) 2002-12-19 2009-09-15 Dsm Ip Assets Bv Neues herstellungsverfahren von lebensmitteln
ES2389442T3 (es) * 2004-02-06 2012-10-26 Novozymes Inc. Polipéptidos con actividad de aumento celulolítica y polinucleótidos que los codifican
CN101107354B (zh) 2005-01-24 2012-05-30 帝斯曼知识产权资产管理有限公司 用于在有丝真菌细胞中生产感兴趣化合物的方法
WO2010005553A1 (en) * 2008-07-07 2010-01-14 Mascoma Corporation Isolation and characterization of schizochytrium aggregatum cellobiohydrolase i (cbh 1)
BRPI1015536A2 (pt) 2009-04-24 2019-07-02 Dsm Ip Assets Bv polipeptídeo de degradação de carboidratos e usos do mesmo

Also Published As

Publication number Publication date
BR112013019514A2 (pt) 2017-03-28
CN103348005B (zh) 2015-03-18
EP2670845A1 (en) 2013-12-11
MX2013008766A (es) 2014-01-31
EA023945B1 (ru) 2016-07-29
US20130309729A1 (en) 2013-11-21
EA201300876A1 (ru) 2013-12-30
WO2012104239A1 (en) 2012-08-09
CN103348005A (zh) 2013-10-09
CA2824494A1 (en) 2012-08-09
US8790894B2 (en) 2014-07-29

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