ES2627334T3 - Endoglucosidasa de streptococcus pyogenes y métodos para usarla - Google Patents

Endoglucosidasa de streptococcus pyogenes y métodos para usarla Download PDF

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Publication number
ES2627334T3
ES2627334T3 ES12756523.2T ES12756523T ES2627334T3 ES 2627334 T3 ES2627334 T3 ES 2627334T3 ES 12756523 T ES12756523 T ES 12756523T ES 2627334 T3 ES2627334 T3 ES 2627334T3
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Spain
Prior art keywords
endos49
igg
polypeptide
seq
amino acid
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ES12756523.2T
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English (en)
Spanish (es)
Inventor
Mattias Collin
Maria Allhorn
Jonathan SJÖGREN
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Genovis AB
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Genovis AB
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01096—Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (3.2.1.96)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56944—Streptococcus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2469/00—Immunoassays for the detection of microorganisms
    • G01N2469/20—Detection of antibodies in sample from host which are directed against antigens from microorganisms

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
ES12756523.2T 2011-09-13 2012-09-12 Endoglucosidasa de streptococcus pyogenes y métodos para usarla Active ES2627334T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB201115841A GB201115841D0 (en) 2011-09-13 2011-09-13 Protein and method
GB201115841 2011-09-13
PCT/EP2012/067841 WO2013037824A1 (en) 2011-09-13 2012-09-12 Endoglycosidase from streptococcus pyogenes and methods using it

Publications (1)

Publication Number Publication Date
ES2627334T3 true ES2627334T3 (es) 2017-07-27

Family

ID=44908522

Family Applications (1)

Application Number Title Priority Date Filing Date
ES12756523.2T Active ES2627334T3 (es) 2011-09-13 2012-09-12 Endoglucosidasa de streptococcus pyogenes y métodos para usarla

Country Status (20)

Country Link
US (1) US9493752B2 (enExample)
EP (1) EP2756077B1 (enExample)
JP (1) JP6076349B2 (enExample)
KR (1) KR102001427B1 (enExample)
CN (1) CN103946377B (enExample)
AU (1) AU2012307461B2 (enExample)
BR (1) BR112014005816A2 (enExample)
CA (1) CA2848230C (enExample)
DK (1) DK2756077T3 (enExample)
EA (1) EA028490B8 (enExample)
ES (1) ES2627334T3 (enExample)
GB (2) GB201115841D0 (enExample)
IL (1) IL231410A0 (enExample)
IN (1) IN2014CN02163A (enExample)
MX (1) MX347727B (enExample)
PL (1) PL2756077T3 (enExample)
PT (1) PT2756077T (enExample)
SG (1) SG11201400505YA (enExample)
WO (1) WO2013037824A1 (enExample)
ZA (1) ZA201401840B (enExample)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1343895B1 (en) * 2000-12-21 2008-06-18 ID Biomedical Corporation Streptococcus pyogenes antigens and corresponding dna fragments
HRP20180268T4 (hr) 2012-10-23 2026-03-27 Synaffix B.V. Modificirano antitijelo, konjugat antitijela i postupak za njihovu pripremu
DK2991683T3 (da) * 2013-05-02 2019-11-04 Glykos Finland Oy Konjugater af et glykoprotein eller en glykan med en toksisk ladning
WO2015057066A1 (en) 2013-10-14 2015-04-23 Synaffix B.V. Glycoengineered antibody, antibody-conjugate and methods for their preparation
WO2015057064A1 (en) 2013-10-14 2015-04-23 Synaffix B.V. Modified glycoprotein, protein-conjugate and process for the preparation thereof
JP2016538877A (ja) 2013-10-14 2016-12-15 シンアフィックス ビー.ブイ. 修飾糖タンパク質、タンパク質コンジュゲート及びそれらの調製方法
WO2015057065A1 (en) 2013-10-14 2015-04-23 Synaffix B.V. Glycoengineered antibody, antibody-conjugate and methods for their preparation
GB201318490D0 (en) * 2013-10-18 2013-12-04 Genovis Ab Method
CN106660967A (zh) 2014-01-24 2017-05-10 西纳福克斯股份有限公司 (杂)芳基1,3‑偶极化合物与(杂)环炔的环加成方法
US10973920B2 (en) 2014-06-30 2021-04-13 Glykos Finland Oy Saccharide derivative of a toxic payload and antibody conjugates thereof
GB201413240D0 (en) 2014-07-25 2014-09-10 Hansa Medical Ab Method
EP3177633B1 (en) 2014-08-04 2022-07-20 SynAffix B.V. Process for the modification of a glycoprotein using a beta-(1,4)-n-acetylgalactosaminyltransferase or a mutant thereof
GB201502306D0 (en) * 2015-02-12 2015-04-01 Hansa Medical Ab Protein
GB201502305D0 (en) * 2015-02-12 2015-04-01 Hansa Medical Ab Protein
CN107810273B (zh) 2015-04-23 2022-09-27 西纳福克斯股份有限公司 用为或衍生自β-(1,4)-N-乙酰半乳糖胺转移酶的糖基转移酶修饰糖蛋白的方法
US11008601B2 (en) * 2016-01-15 2021-05-18 University Of Maryland Endo-S2 mutants as glycosynthases, method of making and use for glycoengineering of glycoproteins
AU2017214441B2 (en) * 2016-02-04 2021-08-19 Genovis Ab New Streptococcal proteases
KR102166120B1 (ko) * 2016-08-24 2020-10-16 초 파마 인크. 당단백질 재구성을 위한 엔도글리코시다제 돌연변이체 및 그의 사용 방법
EP3630968B8 (en) 2017-05-26 2024-07-03 Genovis AB Enzymes for glycan analysis
US20210228738A1 (en) 2017-07-17 2021-07-29 INSERM (Institut National de la Santé et de la Recherche Médicale) Compositions and methods for increasing or enhancing transduction of gene therapy vectors and for removing or reducing immunoglobulins
US20210402002A1 (en) 2018-06-19 2021-12-30 Glykos Biomedical Oy Conjugate
CA3102155A1 (en) 2018-06-29 2020-01-02 Glykos Biomedical Oy Conjugates
US20230038373A1 (en) 2019-12-18 2023-02-09 Glykos Biomedical Oy Stabile conjugate
CN111044731B (zh) * 2019-12-19 2021-05-07 北京科技大学 脉冲温育免疫反应分离富集多肽药物中肽类杂质的方法
BR112022013255A2 (pt) 2020-01-09 2022-09-06 Mersana Therapeutics Inc Conjugados anticorpo-fármaco específicos de sítio com ligantes contendo peptídeo
GB202002072D0 (en) * 2020-02-14 2020-04-01 Hansa Biopharma AB immunoglobulin detection and associated therapies
CN120078887A (zh) * 2020-09-21 2025-06-03 上海宝济药业股份有限公司 一种药物组合及其应用
WO2022147532A1 (en) 2021-01-04 2022-07-07 Mersana Therapeutics, Inc. B7h4-targeted antibody-drug conjugates and methods of use thereof
EP4079848A1 (en) 2021-04-22 2022-10-26 Genovis Ab Immunoglobulin cleaving enzyme
CN115873833B (zh) * 2022-11-08 2024-08-16 上海泰昶生物技术有限公司 生产免疫球蛋白g降解酶的工程菌株和工艺
CN117165643B (zh) * 2023-08-17 2025-10-28 山东大学 一种糖苷内切酶在水解高甘露糖型n-糖蛋白中的应用
TW202519652A (zh) * 2023-11-10 2025-05-16 大陸商上海齊魯製藥研究中心有限公司 新型Endo S2突變體酶
WO2025180451A1 (zh) * 2024-03-01 2025-09-04 上海齐鲁制药研究中心有限公司 新型Endo S2突变体酶
WO2025248500A1 (en) 2024-05-31 2025-12-04 Janssen Biotech, Inc. Antibody drug conjugates that target enpp3
GB202413764D0 (en) 2024-09-18 2024-10-30 Genovis Ab Immunoglobulin proteases and their uses

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0130228D0 (en) 2001-12-18 2002-02-06 Hansa Medica Ab Protein
GB0624874D0 (en) * 2006-12-13 2007-01-24 Hansa Medical Ab Treatment
CN102584989B (zh) * 2007-09-14 2015-05-13 季诺维斯公司 用于解离Fcgamma-受体-IgG复合物及纯化与检测IgG的方法和试剂盒
GB0821100D0 (en) 2008-11-18 2008-12-24 Hansa Medical Ab Antibodies
CN102511007B (zh) * 2009-10-02 2015-04-15 罗切格利卡特公司 抗体中的a-岩藻糖基化检测

Also Published As

Publication number Publication date
PT2756077T (pt) 2017-06-21
DK2756077T3 (en) 2017-07-03
US20140302519A1 (en) 2014-10-09
EP2756077A1 (en) 2014-07-23
BR112014005816A2 (pt) 2020-12-29
SG11201400505YA (en) 2014-04-28
KR20140081813A (ko) 2014-07-01
AU2012307461A1 (en) 2014-03-27
MX347727B (es) 2017-05-10
WO2013037824A1 (en) 2013-03-21
CA2848230C (en) 2020-03-24
ZA201401840B (en) 2018-05-30
EP2756077B1 (en) 2017-03-29
EA028490B1 (ru) 2017-11-30
PL2756077T3 (pl) 2017-09-29
MX2014002906A (es) 2014-10-17
GB2509284A (en) 2014-06-25
CN103946377A (zh) 2014-07-23
IL231410A0 (en) 2014-04-30
GB201406321D0 (en) 2014-05-21
US9493752B2 (en) 2016-11-15
GB201115841D0 (en) 2011-10-26
JP6076349B2 (ja) 2017-02-08
AU2012307461B2 (en) 2016-08-18
IN2014CN02163A (enExample) 2015-05-29
CN103946377B (zh) 2016-10-05
KR102001427B1 (ko) 2019-07-18
NZ622172A (en) 2016-04-29
EA201490447A1 (ru) 2014-09-30
EA028490B8 (ru) 2018-01-31
CA2848230A1 (en) 2013-03-21
JP2014531205A (ja) 2014-11-27

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