JP6023698B2 - 試料からの低濃度の細菌の抽出 - Google Patents
試料からの低濃度の細菌の抽出 Download PDFInfo
- Publication number
- JP6023698B2 JP6023698B2 JP2013506301A JP2013506301A JP6023698B2 JP 6023698 B2 JP6023698 B2 JP 6023698B2 JP 2013506301 A JP2013506301 A JP 2013506301A JP 2013506301 A JP2013506301 A JP 2013506301A JP 6023698 B2 JP6023698 B2 JP 6023698B2
- Authority
- JP
- Japan
- Prior art keywords
- sample
- bacteria
- bacterium
- magnetic
- target
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
- C07K16/1267—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
- G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2446/00—Magnetic particle immunoreagent carriers
- G01N2446/20—Magnetic particle immunoreagent carriers the magnetic material being present in the particle core
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T428/00—Stock material or miscellaneous articles
- Y10T428/29—Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof
- Y10T428/2982—Particulate matter [e.g., sphere, flake, etc.]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Urology & Nephrology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Engineering & Computer Science (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Dispersion Chemistry (AREA)
- Fluid Mechanics (AREA)
- Clinical Laboratory Science (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Description
本願は、2010年4月21日に出願された米国仮特許出願第61/326,588号の利益および優先権を主張し、この米国仮特許出願の全体の内容は、本明細書中に参考として援用される。
本発明は、試料について、試料から細菌を単離するアッセイを実行することに一般に関し、そこで、アッセイは試料中の約1CFU/mlという低い濃度の細菌を単離する。
血液由来病原体は、健康管理上重要な問題である。細菌感染症の診断の遅れまたは不適切な診断は、感染への重大でしばしば致命的な炎症性応答である敗血症をもたらすことがある。敗血症は、米国で10番目の主要な死因である。血液中の細菌感染症の早期発見は、敗血症の開始の阻止での鍵である。血液由来感染症の伝統的な検出および同定の方法には、血液培養および抗生物質感受性アッセイが含まれる。それらの方法は細胞を培養することを一般に必要とし、それは費用がかかり、72時間という長い時間を要することがある。細胞培養結果が得られる前に敗血症性ショックが起こることがしばしばある。
本発明は、生物試料中の病原体を単離するための方法およびデバイスを提供する。本発明は、試料中の非常に低いレベルでの病原体の急速な検出を可能にし、したがって、病原体の早期発見および正確な検出および同定を可能にする。特定の態様では、本発明は、標的特異的結合部分を有する磁気粒子を用いて実施される。本発明の方法は、標的特異的結合部分を含む磁気粒子を体液試料に導入して混合物を作製すること、この混合物をインキュベートして上記粒子を標的に結合させること、磁場を加えて表面上に標的/磁気粒子複合体を捕捉すること、および粒子凝集を低減する洗浄溶液で洗浄し、それによって標的/磁気粒子複合体を単離することを含むことができる。本発明の方法の特別な利点は、多くの臨床試料に存在する低い濃度(血液試料に1CFU/mlという低い濃度の細菌)での、血液試料からの細菌および真菌の直接的な捕捉および単離についてのものである。
本発明の好ましい実施形態において、例えば以下の項目が提供される。
(項目1)
試料から細菌を単離するための方法であって、
細菌を含む試料を得る工程と;
前記試料について、前記試料から前記細菌を単離するアッセイを実行する工程であって、前記アッセイは、前記試料中の約1CFU/mlという低い濃度の細菌を単離する工程と
を含む、方法。
(項目2)
前記試料がヒトの組織または体液である、項目1に記載の方法。
(項目3)
前記体液が血液である、項目2に記載の方法。
(項目4)
前記細菌が血液由来細菌である、項目3に記載の方法。
(項目5)
前記試料が食物試料である、項目1に記載の方法。
(項目6)
前記細菌が食物由来細菌である、項目5に記載の方法。
(項目7)
前記細菌を特徴づける工程をさらに含む、項目1に記載の方法。
(項目8)
特徴づける工程が前記細菌を同定する工程を含む、項目7に記載の方法。
(項目9)
同定する工程が前記細菌に由来する核酸を配列決定する工程、または前記細菌に由来する核酸を増幅する工程から選択される、項目8に記載の方法。
(項目10)
前記細菌がグラム陽性細菌である、項目1に記載の方法。
(項目11)
前記細菌がグラム細菌である、項目1に記載の方法。
(項目12)
試料から細菌を単離するための方法であって、
細菌を含む試料を得る工程と;
前記試料から前記細菌を単離するために磁気粒子を用いてアッセイを行う工程であって、前記磁気粒子は、前記アッセイが前記試料中の約1CFU/mlという低い濃度の細菌を単離するようなサイズであり、かつそのような量の磁性材料を含む工程と
を含む、方法。
(項目13)
前記磁気粒子が細菌特異的結合部分を含む、項目12に記載の方法。
(項目14)
前記細菌特異的結合部分が抗体である、項目13に記載の方法。
(項目15)
前記試料がヒトの組織または体液である、項目12に記載の方法。
(項目16)
前記体液が血液である、項目15に記載の方法。
(項目17)
前記細菌が血液由来細菌である、項目16に記載の方法。
(項目18)
前記試料が食物試料である、項目12に記載の方法。
(項目19)
前記細菌が食物由来細菌である、項目18に記載の方法。
(項目20)
前記細菌を特徴づける工程をさらに含む、項目12に記載の方法。
(項目21)
特徴づける工程が、前記細菌を同定する工程を含む、項目20に記載の方法。
(項目22)
同定する工程が、前記細菌に由来する核酸を配列決定する工程、または前記細菌に由来する核酸を増幅する工程から選択される、項目21に記載の方法。
(項目23)
前記細菌がグラム陽性細菌である、項目12に記載の方法。
(項目24)
前記細菌がグラム細菌である、項目12に記載の方法。
(項目25)
試料から細菌を単離するための方法であって、
細菌を含む試料を得る工程と;
前記試料から前記細菌を単離するために磁気粒子を用いてアッセイを行う工程であって、前記磁気粒子は、前記試料中の約1CFU/mlという低い濃度の細菌の単離を可能にする磁気モーメントを有する工程と
を含む、方法。
本発明は、試料から細菌を単離するアッセイを試料で実行することに一般に関し、そこで、アッセイは試料中の1CFU/mlという低い濃度の細菌を単離する。試料中の1CFU/mlという低い濃度の細菌を単離することができる任意のアッセイを用いることができる。ある態様では、本発明の方法は、体液試料中の標的病原体を捕捉する特定の磁気モーメントを有する磁気粒子(粒径および磁性材料の重量%で決定される)、および標的を単離するための磁石を用いて、試料中の1CFU/mlという低い濃度の細菌を単離する。
この開示全体で、特許、特許出願、特許公報、雑誌、本、論文、ウェブコンテンツなどの他の文書が参照および引用されている。全てのそのような文書は、全ての目的のためにここに参照により完全に本明細書に組み込まれる。
本明細書で示され、記載されるものに加えて、本発明およびその多くのさらなる実施形態の様々な変更は、本明細書で引用される科学文献および特許文献への参照を含むこの文書の全内容から、当業者に明らかになる。本明細書の対象物は、その様々な実施形態および同等物での本発明の実施へ適合させることができる、重要な情報、例証および指針を含む。
健康なボランティアからの血液試料に、血流感染症で最も頻繁に見出される細菌種の研究所株および臨床分離物の両方を含む細菌の臨床上の適切な濃度(1〜10CFU/mL)を混入させた。
ポリクローナルの汎グラム陽性細菌特異的IgGを生み出すために、最初に完全フロイントアジュバントに懸濁させた細菌抗原をリンパ節内に投与し、続いて不完全フロイントアジュバント中の細菌抗原の皮下注射を2週間隔で行うことによってヤギを免疫化した。細菌を指数増殖期(OD600=0.4〜0.8)まで増殖させることによって、抗原を抗体産生のために調製した。遠心による細菌の集菌の後、37℃で4時間の4%ホルムアルデヒドでのホルマリン固定を用いて、細菌を不活性化した。PBSで細菌を3回洗浄した後(15分間洗浄、4000rpmで20分間の遠心)、BCAアッセイを用いて抗原濃度を測定し、免疫化のために抗原を1mg/mLで用いた。グラム陽性細菌特異的IgGを生み出すために、接種のためにいくつかの細菌種を用いた:Staphylococcus aureus、Staphylococcus epidermidis、Enterococcus faeciumおよびEnterococcus fecalis。
超常磁性ビーズは、酸化鉄ナノ粒子(直径5〜15nm)をラテックスコアに封入し、ヤギIgGで標識することによって合成された。有機溶媒にナノ粒子を含む強磁性流体をエタノールで沈殿させ、スチレンおよび界面活性剤Hitenol BC−10の水溶液にナノ粒子を再懸濁させ、超音波処理を用いて乳化した。混合液を撹拌させながら一晩平衡させ、1.2および0.45μmフィルターによって濾過して、均一なミセルサイズを達成した。スチレン、アクリル酸およびジビニルベンゼンを、pH9.6の炭酸バッファーに加えた。K2S2O8の添加により70℃の混合液で重合を開始させ、反応は一晩完了させた。合成された粒子は、磁気捕捉を用いて0.1%SDSで3回洗浄し、1.2、0.8および0.45μmフィルターによって濾過し、抗体コンジュゲーションのために用いた。
血流感染の間に血液に存在する細菌を、上の実施例3で調製される超常磁性ビーズを用いて磁気標識した。実施例1に記載の混入された試料をトリスベースの結合バッファーおよび標的特異的ビーズで3倍に希釈し、その後37℃の振盪プラットホーム上で最大2時間インキュベートした。インキュベーションの後、標識標的を磁気によって分離し、その後血液生成物を除去するように設計された洗浄工程を行った。下の実施例5を参照されたい。
磁気標識標的細菌および過量の遊離ビーズを含む血液を、試料の流れに直角に置かれたいくつかの強力な希土類棒磁石を有するフロースルー捕捉セルに注入した。流路断面0.5mm×20mm(h×w)および7個のNdFeB棒磁石を有するフローチャンバーを用いて、5mL/分という速さの流速が達成された。磁石の存在下で混合液をチャンネル内に流した後に、ヘパリンを含む洗浄溶液をチャンネル内に流した。血液成分を除去して磁気粒子の凝集体の形成を低減するために、結合した標的をヘパリン含有バッファーで1回洗浄した。結合した標的を効果的に洗浄するために、磁石を取り出し、捕捉された磁性材料を洗浄バッファーに再懸濁させ、その後磁場を再び加え、同じフロースルー捕捉セルで磁性材料を捕捉した。
Claims (25)
- 試料から細菌を単離するための方法であって、
細菌を含む試料を得る工程と;
溶解を防止するためのバッファーと、磁気粒子であって、前記細菌に特異的に結合する結合部分を前記磁気粒子に結合させた磁気粒子とを含む混合物中で前記試料をインキュベートし、それによって、細菌/粒子複合体を形成する工程と;
前記磁気粒子に負電荷を付与する溶液中で前記混合物を洗浄する工程と;
フロースルー捕捉セルのチャンネルを通して前記試料を流し、流れている前記試料に磁場を加えることにより、前記細菌/粒子複合体を前記試料中の残りの成分から分離する工程と;
前記混合物から1CFU/mlという低い濃度の前記細菌を単離する工程と
を含む、方法。 - 前記試料がヒトの組織または体液である、請求項1に記載の方法。
- 前記体液が血液である、請求項2に記載の方法。
- 前記細菌が血液由来細菌である、請求項3に記載の方法。
- 前記試料が食物試料である、請求項1に記載の方法。
- 前記細菌が食物由来細菌である、請求項5に記載の方法。
- 前記細菌を特徴づける工程をさらに含む、請求項1に記載の方法。
- 前記特徴づける工程が前記細菌を同定する工程を含む、請求項7に記載の方法。
- 前記細菌がグラム陽性細菌である、請求項1に記載の方法。
- 前記細菌がグラム陰性細菌である、請求項1に記載の方法。
- 試料から細菌を単離するための方法であって、
細菌を含む試料を得る工程と;
細菌特異的結合部分を含む磁気粒子を前記試料に導入する工程と;
粒子凝集を低減し、かつ、細胞溶解を防止する溶液中で前記試料をインキュベートする工程と;
それによって、細菌/粒子複合体を形成する工程と;
前記試料を洗浄する工程と;
フロースルー捕捉セルのチャンネルを通して前記試料を流し、流れている前記試料に磁場を加えることにより、前記細菌/粒子複合体を前記試料中の残りの成分から分離する工程と;
1CFU/mlという低い濃度の前記細菌を単離する工程と
を含む、方法。 - 前記インキュベートする工程が、10秒から2時間行われる、請求項11に記載の方法。
- 前記細菌特異的結合部分が抗体である、請求項11に記載の方法。
- 前記試料がヒトの組織または体液である、請求項11に記載の方法。
- 前記体液が血液である、請求項14に記載の方法。
- 前記細菌が血液由来細菌である、請求項15に記載の方法。
- 前記試料が食物試料である、請求項11に記載の方法。
- 前記細菌が食物由来細菌である、請求項17に記載の方法。
- 前記細菌を特徴づける工程をさらに含む、請求項11に記載の方法。
- 特徴づける工程が、前記細菌を同定する工程を含む、請求項19に記載の方法。
- 同定する工程が、前記細菌に由来する核酸を配列決定する工程、または前記細菌に由来する核酸を増幅する工程から選択される、請求項20に記載の方法。
- 前記細菌がグラム陽性細菌である、請求項11に記載の方法。
- 前記細菌がグラム陰性細菌である、請求項11に記載の方法。
- 検出感度を1CFU/ml〜10CFU/mlの前記細菌という低さとする工程をさらに含む、請求項1に記載の方法。
- 検出感度を1CFU/ml〜10CFU/mlの前記細菌という低さとする工程をさらに含む、請求項11に記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US32658810P | 2010-04-21 | 2010-04-21 | |
US61/326,588 | 2010-04-21 | ||
PCT/US2011/033410 WO2011133759A1 (en) | 2010-04-21 | 2011-04-21 | Extractin low concentrations of bacteria from a sample |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2016198681A Division JP2017012198A (ja) | 2010-04-21 | 2016-10-07 | 試料からの低濃度の細菌の抽出 |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2013524814A JP2013524814A (ja) | 2013-06-20 |
JP2013524814A5 JP2013524814A5 (ja) | 2014-06-19 |
JP6023698B2 true JP6023698B2 (ja) | 2016-11-09 |
Family
ID=44816109
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2013506262A Active JP5814344B2 (ja) | 2010-04-21 | 2011-04-20 | 体液からの標的分析物の単離 |
JP2013506301A Active JP6023698B2 (ja) | 2010-04-21 | 2011-04-21 | 試料からの低濃度の細菌の抽出 |
JP2016198681A Pending JP2017012198A (ja) | 2010-04-21 | 2016-10-07 | 試料からの低濃度の細菌の抽出 |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2013506262A Active JP5814344B2 (ja) | 2010-04-21 | 2011-04-20 | 体液からの標的分析物の単離 |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2016198681A Pending JP2017012198A (ja) | 2010-04-21 | 2016-10-07 | 試料からの低濃度の細菌の抽出 |
Country Status (6)
Country | Link |
---|---|
US (11) | US20110262989A1 (ja) |
EP (5) | EP2561360B9 (ja) |
JP (3) | JP5814344B2 (ja) |
CA (4) | CA2796767C (ja) |
ES (3) | ES2774393T3 (ja) |
WO (4) | WO2011133630A1 (ja) |
Families Citing this family (43)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010068812A1 (en) | 2008-12-10 | 2010-06-17 | Abqmr, Inc. | Nuclear magnetic resonance apparatus, methods and associated technology |
US20110262989A1 (en) | 2010-04-21 | 2011-10-27 | Nanomr, Inc. | Isolating a target analyte from a body fluid |
US9428547B2 (en) | 2010-04-21 | 2016-08-30 | Dna Electronics, Inc. | Compositions for isolating a target analyte from a heterogeneous sample |
US20130109590A1 (en) * | 2010-04-21 | 2013-05-02 | Lisa-Jo Ann CLARIZIA | Isolating a target analyte from a body fluid |
US9476812B2 (en) | 2010-04-21 | 2016-10-25 | Dna Electronics, Inc. | Methods for isolating a target analyte from a heterogeneous sample |
US20140100136A1 (en) * | 2010-04-21 | 2014-04-10 | Nanomr, Inc. | Isolation and characterization of pathogens |
US8841104B2 (en) | 2010-04-21 | 2014-09-23 | Nanomr, Inc. | Methods for isolating a target analyte from a heterogeneous sample |
GB201100515D0 (en) * | 2011-01-13 | 2011-02-23 | Matrix Microscience Ltd | Methods of capturing bindable targets from liquids |
US9099233B2 (en) * | 2011-09-23 | 2015-08-04 | Uchicago Argonne, Llc | Interface colloidal robotic manipulator |
CN103389241A (zh) * | 2012-05-10 | 2013-11-13 | 中国科学院理化技术研究所 | 一种血液纯化的方法 |
KR101523822B1 (ko) | 2012-11-26 | 2015-05-28 | 포항공과대학교 산학협력단 | 자성 나노입자와 고점성(高粘性) 용액을 이용한 식중독균 검출방법 |
US10156567B2 (en) | 2012-12-17 | 2018-12-18 | General Electric Company | In-vitro magnetic resonance detection of a target substance without separating bound magnetic nanoparticles from unbound magnetic nanoparticles |
US9995742B2 (en) | 2012-12-19 | 2018-06-12 | Dnae Group Holdings Limited | Sample entry |
US9804069B2 (en) | 2012-12-19 | 2017-10-31 | Dnae Group Holdings Limited | Methods for degrading nucleic acid |
US10000557B2 (en) | 2012-12-19 | 2018-06-19 | Dnae Group Holdings Limited | Methods for raising antibodies |
WO2014100456A1 (en) * | 2012-12-19 | 2014-06-26 | Nanomr, Inc. | Target capture system |
US9551704B2 (en) | 2012-12-19 | 2017-01-24 | Dna Electronics, Inc. | Target detection |
US9434940B2 (en) | 2012-12-19 | 2016-09-06 | Dna Electronics, Inc. | Methods for universal target capture |
US9599610B2 (en) | 2012-12-19 | 2017-03-21 | Dnae Group Holdings Limited | Target capture system |
US20140170667A1 (en) * | 2012-12-19 | 2014-06-19 | Nanomr, Inc. | Methods for amplifying nucleic acid from a target |
US10732178B2 (en) * | 2013-01-29 | 2020-08-04 | Bio-Rad Haifa Ltd. | Detection assays employing magnetic nanoparticles |
US10350320B2 (en) | 2013-01-29 | 2019-07-16 | Children's Medical Center Corporation | Magnetic separation using nanoparticles |
WO2014144340A1 (en) * | 2013-03-15 | 2014-09-18 | Ancera, Inc. | Systems and methods for three-dimensional extraction of target particles ferrofluids |
WO2014145765A1 (en) | 2013-03-15 | 2014-09-18 | Ancera, Inc. | Systems and methods for bead-based assays in ferrofluids |
WO2014144782A2 (en) | 2013-03-15 | 2014-09-18 | Ancera, Inc. | Systems and methods for active particle separation |
JP6252933B2 (ja) * | 2013-09-17 | 2017-12-27 | 学校法人上智学院 | 構造体ならびにこれを用いた細菌の捕集および検出方法 |
US20150119285A1 (en) * | 2013-10-28 | 2015-04-30 | Chin-Yih Hong | Magnetic-assisted rapid aptamer selection method for generating high affinity dna aptamer |
CN104655838A (zh) * | 2013-11-19 | 2015-05-27 | 北京市理化分析测试中心 | 一种检测样品中金黄色葡萄球菌活菌体的方法 |
DE102014206444A1 (de) * | 2014-04-03 | 2015-10-08 | Siemens Aktiengesellschaft | Verfahren für die Molekulardiagnostik zum Anreichern einer Nukleinsäure aus einer biologischen Probe |
US11285490B2 (en) | 2015-06-26 | 2022-03-29 | Ancera, Llc | Background defocusing and clearing in ferrofluid-based capture assays |
US10151749B2 (en) * | 2015-08-05 | 2018-12-11 | Alfaisal University | Method and kit for the detection of microorganisms |
CA2997546A1 (en) | 2015-09-14 | 2017-03-23 | Medisieve Ltd | Magnetic filter apparatus |
CN108351347A (zh) * | 2015-09-22 | 2018-07-31 | 普渡研究基金会 | 稀有细胞的无离心机分离和检测 |
EP3373950B1 (en) | 2015-11-11 | 2024-05-01 | Serimmune Inc. | Methods and compositions for assessing antibody specificities |
CN109414710B (zh) | 2016-07-28 | 2021-02-05 | 医疗过滤有限公司 | 磁力混合器及方法 |
US10845449B2 (en) | 2016-10-20 | 2020-11-24 | Quantum Diamond Technologies Inc. | Methods and apparatus for magnetic particle analysis using diamond magnetic imaging |
US11435371B2 (en) * | 2016-11-24 | 2022-09-06 | Siemens Healthineers Nederland B.V. | Device, system method and kit for isolating an analyte from a body fluid sample |
EP3559668B1 (en) * | 2016-12-23 | 2023-07-19 | Quantum Diamond Technologies Inc. | Methods and apparatus for magnetic multi-bead assays |
EP3662293B1 (en) | 2017-07-31 | 2022-09-07 | Quantum Diamond Technologies Inc. | Sensor system comprising a sample cartridge including a flexible membrane for supporting a sample |
WO2019031815A1 (ko) * | 2017-08-07 | 2019-02-14 | 울산과학기술원 | 자성 입자를 이용한 유체 분리 시스템 및 방법 |
US11154828B2 (en) * | 2018-09-14 | 2021-10-26 | Uchicago Argonne, Llc | Turbulent mixing by microscopic self-assembled spinners |
EP4004203A4 (en) * | 2019-07-26 | 2023-07-26 | Becton, Dickinson and Company | BUFFER COMPOSITIONS TO REDUCE CLUSTERING |
KR102402112B1 (ko) | 2020-04-27 | 2022-05-25 | 한국식품연구원 | 메타 구조체를 이용한 품질 분석용 나노 센서 |
Family Cites Families (306)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4180563A (en) * | 1971-12-24 | 1979-12-25 | Institut Pasteur | Immunostimulant agent from Salmonella typhimurium or Listeria monocytogenes bacterial cells and pharmaceutical composition |
US4018886A (en) | 1975-07-01 | 1977-04-19 | General Electric Company | Diagnostic method and device employing protein-coated magnetic particles |
US3970518A (en) | 1975-07-01 | 1976-07-20 | General Electric Company | Magnetic separation of biological particles |
US4267234A (en) | 1978-03-17 | 1981-05-12 | California Institute Of Technology | Polyglutaraldehyde synthesis and protein bonding substrates |
US4230685A (en) | 1979-02-28 | 1980-10-28 | Northwestern University | Method of magnetic separation of cells and the like, and microspheres for use therein |
US4434237A (en) * | 1982-03-31 | 1984-02-28 | Dinarello Charles A | Human leukocytic pyrogen test for the detection of exogenous fever-producing substances |
US4452773A (en) | 1982-04-05 | 1984-06-05 | Canadian Patents And Development Limited | Magnetic iron-dextran microspheres |
US4659678A (en) | 1982-09-29 | 1987-04-21 | Serono Diagnostics Limited | Immunoassay of antigens |
FR2537725B1 (fr) | 1982-12-09 | 1985-07-12 | Pasteur Institut | Procede de detection immunobacteriologique de germes pathogenes dans des milieux biologiques contamines |
ATE98300T1 (de) | 1983-01-10 | 1993-12-15 | Gen Probe Inc | Verfahren zum aufspueren, identifizieren und quantifizieren von organismen und viren. |
US4554088A (en) | 1983-05-12 | 1985-11-19 | Advanced Magnetics Inc. | Magnetic particles for use in separations |
US4695393A (en) | 1983-05-12 | 1987-09-22 | Advanced Magnetics Inc. | Magnetic particles for use in separations |
US4551435A (en) | 1983-08-24 | 1985-11-05 | Immunicon, Inc. | Selective removal of immunospecifically recognizable substances from solution |
US5242794A (en) | 1984-12-13 | 1993-09-07 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US5597531A (en) | 1985-10-04 | 1997-01-28 | Immunivest Corporation | Resuspendable coated magnetic particles and stable magnetic particle suspensions |
US4795698A (en) | 1985-10-04 | 1989-01-03 | Immunicon Corporation | Magnetic-polymer particles |
US5512332A (en) | 1985-10-04 | 1996-04-30 | Immunivest Corporation | Process of making resuspendable coated magnetic particles |
US5583024A (en) | 1985-12-02 | 1996-12-10 | The Regents Of The University Of California | Recombinant expression of Coleoptera luciferase |
US4925788A (en) | 1986-10-24 | 1990-05-15 | Immunicon Corporation | Immunoassay system and procedure based on precipitin-like interaction between immune complex and Clq or other non-immunospecific factor |
ES2112824T5 (es) | 1986-11-24 | 2008-03-01 | Gen-Probe Incorporated | Sondas de acidos nucleicos para deteccion y/o cuantificacion de organismos no virales. |
US5136095A (en) | 1987-05-19 | 1992-08-04 | Syntex (U.S.A.) Inc. | Reversible agglutination mediators |
US4901018A (en) | 1987-06-01 | 1990-02-13 | Lew Hyok S | Nuclear magnetic resonance net organic flowmeter |
US5149625A (en) | 1987-08-11 | 1992-09-22 | President And Fellows Of Harvard College | Multiplex analysis of DNA |
US4942124A (en) | 1987-08-11 | 1990-07-17 | President And Fellows Of Harvard College | Multiplex sequencing |
US5089386A (en) | 1987-09-11 | 1992-02-18 | Gene-Trak Systems | Test for listeria |
US5004699A (en) | 1987-11-20 | 1991-04-02 | Sri International | Method to detect fungi and yeasts |
WO1989006699A1 (en) | 1988-01-13 | 1989-07-27 | Institut Pasteur | Dna probe specific for hemolytic listeria |
US4988617A (en) | 1988-03-25 | 1991-01-29 | California Institute Of Technology | Method of detecting a nucleotide change in nucleic acids |
US5057413A (en) | 1988-06-13 | 1991-10-15 | Becton, Dickinson And Company | Method for discriminating between intact and damaged cells in a sample |
US5047321A (en) | 1988-06-15 | 1991-09-10 | Becton Dickinson & Co. | Method for analysis of cellular components of a fluid |
US5108933A (en) | 1988-09-16 | 1992-04-28 | Immunicon Corporation | Manipulation of colloids for facilitating magnetic separations |
CA2025236A1 (en) | 1989-02-06 | 1990-08-07 | Walter King | Probes and methods for the detection of listeria |
US5698271A (en) | 1989-08-22 | 1997-12-16 | Immunivest Corporation | Methods for the manufacture of magnetically responsive particles |
GB8927744D0 (en) | 1989-12-07 | 1990-02-07 | Diatec A S | Process and apparatus |
DE4002160A1 (de) | 1990-01-25 | 1991-08-08 | Bruker Analytische Messtechnik | Probenkopf fuer kernresonanzmessungen und verfahren zur messung von kernresonanzen |
US5840580A (en) | 1990-05-01 | 1998-11-24 | Becton Dickinson And Company | Phenotypic characterization of the hematopoietic stem cell |
US5622853A (en) | 1990-05-01 | 1997-04-22 | Becton Dickinson And Company | T lymphocyte precursor |
US5254460A (en) | 1990-05-03 | 1993-10-19 | Advanced Magnetics, Inc. | Solvent mediated relaxation assay system |
US5164297A (en) | 1990-05-03 | 1992-11-17 | Advanced Magnetics Inc. | Solvent mediated relaxation assay system |
US5494810A (en) | 1990-05-03 | 1996-02-27 | Cornell Research Foundation, Inc. | Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease |
US5270163A (en) | 1990-06-11 | 1993-12-14 | University Research Corporation | Methods for identifying nucleic acid ligands |
US5200084A (en) | 1990-09-26 | 1993-04-06 | Immunicon Corporation | Apparatus and methods for magnetic separation |
US5622831A (en) | 1990-09-26 | 1997-04-22 | Immunivest Corporation | Methods and devices for manipulation of magnetically collected material |
US6013532A (en) | 1990-09-26 | 2000-01-11 | Immunivest Corporation | Methods for magnetic immobilization and manipulation of cells |
US5541072A (en) | 1994-04-18 | 1996-07-30 | Immunivest Corporation | Method for magnetic separation featuring magnetic particles in a multi-phase system |
NL9002696A (nl) | 1990-11-15 | 1992-06-01 | U Gene Research Bv | Werkwijze en kit voor het aantonen van microoerganismen. |
US5491068A (en) | 1991-02-14 | 1996-02-13 | Vicam, L.P. | Assay method for detecting the presence of bacteria |
WO1992015883A1 (en) | 1991-03-08 | 1992-09-17 | Amoco Corporation | Improved assays including colored organic compounds |
US5795470A (en) | 1991-03-25 | 1998-08-18 | Immunivest Corporation | Magnetic separation apparatus |
US5186827A (en) | 1991-03-25 | 1993-02-16 | Immunicon Corporation | Apparatus for magnetic separation featuring external magnetic means |
US5466574A (en) | 1991-03-25 | 1995-11-14 | Immunivest Corporation | Apparatus and methods for magnetic separation featuring external magnetic means |
US5646001A (en) | 1991-03-25 | 1997-07-08 | Immunivest Corporation | Affinity-binding separation and release of one or more selected subset of biological entities from a mixed population thereof |
GB9107124D0 (en) | 1991-04-05 | 1991-05-22 | Dynal As | Chemical process |
US5234816A (en) | 1991-07-12 | 1993-08-10 | Becton, Dickinson And Company | Method for the classification and monitoring of leukemias |
CA2087086A1 (en) | 1992-01-22 | 1993-07-23 | Leon Wmm Terstappen | Multidimensional cell differential analysis |
US5869252A (en) | 1992-03-31 | 1999-02-09 | Abbott Laboratories | Method of multiplex ligase chain reaction |
US6100099A (en) | 1994-09-06 | 2000-08-08 | Abbott Laboratories | Test strip having a diagonal array of capture spots |
US5338687A (en) | 1992-09-11 | 1994-08-16 | Lee Lawrence L | Detection of biological macromolecules by NMR-sensitive labels |
US5342790A (en) | 1992-10-30 | 1994-08-30 | Becton Dickinson And Company | Apparatus for indirect fluorescent assay of blood samples |
EP0610774B1 (en) | 1993-02-09 | 2001-03-28 | Becton, Dickinson and Company | Automatic lineage assignment of acute leukemias by flow cytometry |
CA2156174A1 (en) | 1993-02-17 | 1994-09-01 | Bruce Oberhardt | Dry chemistry cascade immunoassay and affinity assay |
US5702936A (en) * | 1993-05-27 | 1997-12-30 | Icos Corporation | Cyclic GMP-binding, cyclic GMP-specific phosphodiesterase materials and methods |
US5445936A (en) * | 1993-09-15 | 1995-08-29 | Ciba Corning Diagnostics Corp. | Method for non-competitive binding assays |
FR2710410B1 (fr) | 1993-09-20 | 1995-10-20 | Bio Merieux | Procédé et dispositif pour la détermination d'un analyte dans un échantillon . |
US6555324B1 (en) | 1993-11-04 | 2003-04-29 | Becton Dickinson & Company | Method to distinguish hematopoietic progenitor cells |
EP0773203B1 (en) | 1994-05-17 | 2000-01-19 | Asahi Chemical Company, Limited | Glazing layer forming composition for hot coating of oven refractory and method of forming glazing layer |
US5607846A (en) | 1994-05-17 | 1997-03-04 | Research Foundation Of State University Of New York | Vaccine for moraxella catarrhalis |
AU2636995A (en) * | 1994-05-18 | 1995-12-05 | Research And Development Institute, Inc. | Simple, rapid method for the detection, identification and enumeration of specific viable microorganisms |
US5942391A (en) * | 1994-06-22 | 1999-08-24 | Mount Sinai School Of Medicine | Nucleic acid amplification method: ramification-extension amplification method (RAM) |
JP3607320B2 (ja) | 1994-09-02 | 2005-01-05 | 株式会社日立製作所 | 微粒子を用いた分析における固相の回収方法及び装置 |
US5846719A (en) | 1994-10-13 | 1998-12-08 | Lynx Therapeutics, Inc. | Oligonucleotide tags for sorting and identification |
US5695934A (en) | 1994-10-13 | 1997-12-09 | Lynx Therapeutics, Inc. | Massively parallel sequencing of sorted polynucleotides |
US5604097A (en) | 1994-10-13 | 1997-02-18 | Spectragen, Inc. | Methods for sorting polynucleotides using oligonucleotide tags |
US5654636A (en) | 1994-11-14 | 1997-08-05 | The Board Of Trustees Of The University Of Illinois | Method and apparatus for NMR spectroscopy of nanoliter volume samples |
US5935825A (en) | 1994-11-18 | 1999-08-10 | Shimadzu Corporation | Process and reagent for amplifying nucleic acid sequences |
US6097188A (en) | 1995-01-31 | 2000-08-01 | The Board Of Trustees Of The University Of Illinois | Microcoil based micro-NMR spectrometer and method |
US6884357B2 (en) | 1995-02-21 | 2005-04-26 | Iqbal Waheed Siddiqi | Apparatus and method for processing magnetic particles |
FR2732116B1 (fr) | 1995-03-21 | 1997-05-09 | Bio Merieux | Procede et dispositif pour la determination qualitative et/ou quantitative d'un analyte, notamment d'une bacterie, dans un echantillon, par voie magnetique |
US6265150B1 (en) | 1995-06-07 | 2001-07-24 | Becton Dickinson & Company | Phage antibodies |
US6495357B1 (en) * | 1995-07-14 | 2002-12-17 | Novozyme A/S | Lipolytic enzymes |
US5741714A (en) | 1995-07-18 | 1998-04-21 | Immunivest Corporation | Detection of bound analyte by magnetic partitioning and masking |
US5636400A (en) | 1995-08-07 | 1997-06-10 | Young; Keenan L. | Automatic infant bottle cleaner |
US5660990A (en) | 1995-08-18 | 1997-08-26 | Immunivest Corporation | Surface immobilization of magnetically collected materials |
EP0990175A4 (en) | 1995-12-29 | 2000-06-14 | Doty Scient Inc | LOW-INDUCTANCE DIVIDED WIRE REINFORCEMENT COIL IN THE CROSSWAY |
US5684401A (en) | 1996-02-01 | 1997-11-04 | Board Of Trustees Of The University Of Illinois | Apparatus and method for compensation of magnetic susceptibility variation in NMR microspectroscopy detection microcoils |
JP3749959B2 (ja) * | 1996-02-26 | 2006-03-01 | 株式会社シノテスト | 磁性粒子を用いる被検物質の測定方法及び該方法に使用する測定器具 |
US6790366B2 (en) | 1996-06-07 | 2004-09-14 | Immunivest Corporation | Magnetic separation apparatus and methods |
US6136182A (en) | 1996-06-07 | 2000-10-24 | Immunivest Corporation | Magnetic devices and sample chambers for examination and manipulation of cells |
US7666308B2 (en) | 1996-06-07 | 2010-02-23 | Veridex, Llc. | Magnetic separation apparatus and methods |
US5985153A (en) | 1996-06-07 | 1999-11-16 | Immunivest Corporation | Magnetic separation apparatus and methods employing an internal magnetic capture gradient and an external transport force |
US6660159B1 (en) | 1996-06-07 | 2003-12-09 | Immunivest Corporation | Magnetic separation apparatus and methods |
US6890426B2 (en) | 1996-06-07 | 2005-05-10 | Immunivest Corporation | Magnetic separation apparatus and methods |
US20030054376A1 (en) | 1997-07-07 | 2003-03-20 | Mullis Kary Banks | Dual bead assays using cleavable spacers and/or ligation to improve specificity and sensitivity including related methods and apparatus |
US6383763B1 (en) * | 1996-07-26 | 2002-05-07 | Case Western Reserve University | Detection of mycobacteria |
US6361944B1 (en) | 1996-07-29 | 2002-03-26 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
WO1998005791A1 (en) | 1996-08-02 | 1998-02-12 | Immunivest Corporation | Method to select and transfect cell subpopulations |
WO1998020148A1 (en) * | 1996-11-04 | 1998-05-14 | The Regents Of The University Of California | Method for detection of pathogens in food |
US5768089A (en) | 1997-01-10 | 1998-06-16 | Varian Associates, Inc. | Variable external capacitor for NMR probe |
US6146838A (en) | 1997-03-18 | 2000-11-14 | Igen International, Inc. | Detecting water-borne parasites using electrochemiluminescence |
US6623983B1 (en) | 1997-03-25 | 2003-09-23 | Immunivest Corporation | Apparatus and methods for capture and analysis of particulate entities |
CA2285918C (en) | 1997-04-04 | 2007-07-03 | Biosite Diagnostics | Methods for concentrating ligands using magnetic particles |
US6587706B1 (en) | 1997-08-22 | 2003-07-01 | Image-Guided Drug Delivery Systems, Inc. | Microcoil device with a forward field-of-view for large gain magnetic resonance imaging |
US6046585A (en) | 1997-11-21 | 2000-04-04 | Quantum Design, Inc. | Method and apparatus for making quantitative measurements of localized accumulations of target particles having magnetic particles bound thereto |
JPH11169194A (ja) * | 1997-12-09 | 1999-06-29 | Kikkoman Corp | 免疫的微生物検出用生物発光試薬および免疫的微生物検出法 |
DE19755782A1 (de) | 1997-12-16 | 1999-06-17 | Philips Patentverwaltung | MR-Anordnung mit einem medizinischen Instrument und Verfahren zur Positionsbestimmung des medizinischen Instruments |
EP1042061A1 (en) | 1997-12-24 | 2000-10-11 | Cepheid | Integrated fluid manipulation cartridge |
DE19800471A1 (de) | 1998-01-09 | 1999-07-15 | Philips Patentverwaltung | MR-Verfahren mit im Untersuchungsbereich befindlichen Mikrospulen |
KR100399475B1 (ko) | 1998-02-12 | 2003-09-29 | 보드 오브 리전츠, 더 유니버시티 오브 텍사스 시스템 | 순환 중인 암세포의 신속하고 효과적인 분리 방법 및 이를위한 제제 |
US7282350B2 (en) | 1998-02-12 | 2007-10-16 | Immunivest Corporation | Labeled cell sets for use as functional controls in rare cell detection assays |
US20010018192A1 (en) | 1998-02-12 | 2001-08-30 | Terstappen Leon W.M.M. | Labeled cells for use as an internal functional control in rare cell detection assays |
US20020172987A1 (en) | 1998-02-12 | 2002-11-21 | Terstappen Leon W.M.M. | Methods and reagents for the rapid and efficient isolation of circulating cancer cells |
US6036857A (en) * | 1998-02-20 | 2000-03-14 | Florida State University Research Foundation, Inc. | Apparatus for continuous magnetic separation of components from a mixture |
AU3458299A (en) * | 1998-03-31 | 1999-10-18 | United States Of America, As Represented By The Secretary Of Agriculture, The | Monoclonal antibodies against (campylobacter jejuni) and (campylobacter coli) outer membrane antigens |
EP1068532A4 (en) * | 1998-04-15 | 2003-07-16 | Univ Utah State | DETECTION OF REAL-TIME ANTIGENS |
US7078224B1 (en) | 1999-05-14 | 2006-07-18 | Promega Corporation | Cell concentration and lysate clearance using paramagnetic particles |
AU3845099A (en) | 1998-05-15 | 1999-12-06 | Robin Medical Inc. | Method and apparatus for generating controlled torques on objects particularly objects inside a living body |
US6194900B1 (en) | 1998-06-19 | 2001-02-27 | Agilent Technologies, Inc. | Integrated miniaturized device for processing and NMR detection of liquid phase samples |
US6361749B1 (en) | 1998-08-18 | 2002-03-26 | Immunivest Corporation | Apparatus and methods for magnetic separation |
US6469636B1 (en) | 1998-12-02 | 2002-10-22 | Halliburton Energy Services, Inc. | High-power well logging method and apparatus |
US6551843B1 (en) | 1999-01-29 | 2003-04-22 | Immunivest Corporation | Methods for enhancing binding interactions between members of specific binding pairs |
WO2000050924A1 (en) | 1999-02-26 | 2000-08-31 | Purdue Research Foundation | Nuclear magnetic resonance analysis of multiple samples |
US6326787B1 (en) | 1999-06-10 | 2001-12-04 | Sandia National Laboratories | NMR of thin layers using a meanderline surface coil |
US7523385B2 (en) | 1999-06-22 | 2009-04-21 | Starcite, Inc. | System and method for enterprise event marketing and management automation |
US6818395B1 (en) | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
US6623982B1 (en) | 1999-07-12 | 2003-09-23 | Immunivest Corporation | Increased separation efficiency via controlled aggregation of magnetic nanoparticles |
EP1210358A4 (en) | 1999-08-13 | 2005-01-05 | Univ Brandeis | PROOF OF NUCLEIC ACIDS |
EP1204869B1 (en) | 1999-08-17 | 2008-10-22 | Luminex Corporation | Method for analyzing a number of samples from a variety of sources for a single analyte |
CA2382221A1 (en) * | 1999-08-20 | 2001-03-01 | H. Shaw Warren | Outer membrane protein a, peptidoglycan-associated lipoprotein, and murein lipoprotein as therapeutic targets for treatment of sepsis |
US6242915B1 (en) | 1999-08-27 | 2001-06-05 | General Electric Company | Field-frequency lock system for magnetic resonance system |
US6898430B1 (en) | 1999-10-27 | 2005-05-24 | Telecordia Technologies, Inc. | Methods for establishing reliable communications between two points in a mobile wireless network |
US6307372B1 (en) | 1999-11-02 | 2001-10-23 | Glaxo Wellcome, Inc. | Methods for high throughput chemical screening using magnetic resonance imaging |
DE19956595A1 (de) | 1999-11-25 | 2001-05-31 | Philips Corp Intellectual Pty | MR-Verfahren zur Anregung der Kernmagnetisierung in einem begrenzten räumlichen Bereich |
JP2003534773A (ja) | 1999-12-03 | 2003-11-25 | ザイモジェネティクス,インコーポレイティド | ヒトサイトカイン受容体 |
US6582938B1 (en) | 2001-05-11 | 2003-06-24 | Affymetrix, Inc. | Amplification of nucleic acids |
US6514415B2 (en) | 2000-01-31 | 2003-02-04 | Dexter Magnetic Technologies, Inc. | Method and apparatus for magnetic separation of particles |
EP1139110B1 (en) | 2000-03-21 | 2009-11-11 | Image-Guided Neurologics, Inc. | Device with field-modifying structure |
AU2001250925A1 (en) | 2000-03-21 | 2001-10-03 | Telcordia Technologies, Inc. | Combined adaptive spatio-temporal processing and multi-user detection for cdma wireless systems |
US6487437B1 (en) | 2000-03-21 | 2002-11-26 | Image-Guided Neurologies, Inc. | Device for high gain and uniformly localized magnetic resonance imaging |
US6845262B2 (en) | 2000-03-29 | 2005-01-18 | The Brigham And Women's Hospital, Inc. | Low-field MRI |
WO2001073460A1 (en) | 2000-03-30 | 2001-10-04 | Koninklijke Philips Electronics N.V. | Magnetic resonance imaging utilizing a microcoil |
US6456072B1 (en) | 2000-05-26 | 2002-09-24 | The Board Of Trustees Of The University Of Illinois | Method and apparatus for simultaneous acquisition of high resolution NMR spectra from multiple samples |
US6788061B1 (en) | 2000-08-01 | 2004-09-07 | The Board Of Trustees Of The University Of Illinois | Microcoil based micro-NMR spectrometer and method |
US6610499B1 (en) * | 2000-08-31 | 2003-08-26 | The Regents Of The University Of California | Capillary array and related methods |
US20020164659A1 (en) | 2000-11-30 | 2002-11-07 | Rao Galla Chandra | Analytical methods and compositions |
WO2002056049A2 (en) | 2000-12-01 | 2002-07-18 | Protasis Corp | Microfluidic device with multiple microcoil nmr detectors |
US6700379B2 (en) | 2000-12-01 | 2004-03-02 | Protasis Corporation | Steep solvent gradient NMR analysis method |
CA2445509A1 (en) | 2001-01-25 | 2002-12-19 | James D. Thacker | Rapid methods for microbial typing and enumeration |
US6867021B2 (en) | 2001-02-20 | 2005-03-15 | Board Of Trustees Of Michigan State University | Multiplex RT-PCR/PCR for simultaneous detection of bovine coronavirus, bovine rotavirus, Cryptosporidium parvum, and Escherichia coli |
CN100451651C (zh) * | 2001-03-09 | 2009-01-14 | 株式会社三菱化学药得论 | 测试全血的方法 |
WO2002072892A1 (en) | 2001-03-12 | 2002-09-19 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension |
US7200430B2 (en) | 2001-03-29 | 2007-04-03 | The Regents Of The University Of California | Localized two-dimensional shift correlated MR spectroscopy of human brain |
US6404193B1 (en) | 2001-04-09 | 2002-06-11 | Waters Investments Limited | Solvent susceptibility compensation for coupled LC-NMR |
AU2002320058A1 (en) | 2001-06-06 | 2002-12-16 | The General Hspital Corporation | Magnetic-nanoparticle conjugates and methods of use |
US6905885B2 (en) | 2001-06-12 | 2005-06-14 | The Regents Of The University Of California | Portable pathogen detection system |
US20020192676A1 (en) * | 2001-06-18 | 2002-12-19 | Madonna Angelo J. | Method for determining if a type of bacteria is present in a mixture |
US7494771B2 (en) | 2001-07-19 | 2009-02-24 | Geneohm Sciences Canada, Inc. | Universal method and composition for the rapid lysis of cells for the release of nucleic acids and their detection |
US7863012B2 (en) | 2004-02-17 | 2011-01-04 | Veridex, Llc | Analysis of circulating tumor cells, fragments, and debris |
CN100354430C (zh) | 2001-09-06 | 2007-12-12 | 基因描绘系统有限公司 | 一种检测样品中靶细胞或病毒的方法 |
DE10151779A1 (de) | 2001-10-19 | 2003-05-08 | Philips Corp Intellectual Pty | Verfahren zum Lokalisieren eines Gegenstandes in einer MR-Apparatur sowie Katheter und MR-Apparatur zur Durchführung des Verfahrens |
US7148683B2 (en) | 2001-10-25 | 2006-12-12 | Intematix Corporation | Detection with evanescent wave probe |
ATE348104T1 (de) * | 2001-10-29 | 2007-01-15 | Univ Mcgill | Azyklische linker enthaltende oligonukleotide und deren verwendungen |
CA2759764C (en) | 2002-02-14 | 2017-06-13 | Veridex, Llc | Methods and algorithms for cell enumeration in a low-cost cytometer |
US7764821B2 (en) | 2002-02-14 | 2010-07-27 | Veridex, Llc | Methods and algorithms for cell enumeration in a low-cost cytometer |
US6798200B2 (en) | 2002-06-03 | 2004-09-28 | Long-Sheng Fan | Batch-fabricated gradient and RF coils for submicrometer resolution magnetic resonance imaging and manipulation |
US20040018611A1 (en) | 2002-07-23 | 2004-01-29 | Ward Michael Dennis | Microfluidic devices for high gradient magnetic separation |
US7096057B2 (en) | 2002-08-02 | 2006-08-22 | Barnes Jewish Hospital | Method and apparatus for intracorporeal medical imaging using a self-tuned coil |
JP4212331B2 (ja) | 2002-10-24 | 2009-01-21 | 株式会社日立メディコ | 磁気共鳴イメージング装置及び超電導磁石装置 |
US7011794B2 (en) | 2002-11-25 | 2006-03-14 | Immunivest Corporation | Upon a cartridge for containing a specimen sample for optical analysis |
US7037343B2 (en) | 2002-12-23 | 2006-05-02 | Python, Inc. | Stomach prosthesis |
US7338637B2 (en) | 2003-01-31 | 2008-03-04 | Hewlett-Packard Development Company, L.P. | Microfluidic device with thin-film electronic devices |
US6876200B2 (en) | 2003-03-31 | 2005-04-05 | Varian, Inc. | NMR probe having an inner quadrature detection coil combined with a spiral wound outer coil for irradiation |
GB0313259D0 (en) | 2003-06-09 | 2003-07-16 | Consejo Superior Investigacion | Magnetic nanoparticles |
US7282180B2 (en) | 2003-07-02 | 2007-10-16 | Immunivest Corporation | Devices and methods to image objects |
US7422296B2 (en) | 2003-07-07 | 2008-09-09 | Ws Packaging Group, Inc. | Sheet dispenser display strip |
EP1660858A4 (en) * | 2003-07-21 | 2007-10-24 | Amplified Proteomics Inc | MULTIPLEX analyte |
GB0319671D0 (en) | 2003-08-21 | 2003-09-24 | Secr Defence | Apparatus for processing a fluid sample |
US7682833B2 (en) | 2003-09-10 | 2010-03-23 | Abbott Point Of Care Inc. | Immunoassay device with improved sample closure |
US8509875B2 (en) | 2003-09-12 | 2013-08-13 | Koninklijke Philips N.V. | Process for locating a medical instrument with a microcoil |
US20050069900A1 (en) | 2003-09-25 | 2005-03-31 | Cytyc Corporation | Analyte sample detection |
US7169560B2 (en) | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
US20050181353A1 (en) | 2004-02-17 | 2005-08-18 | Rao Galla C. | Stabilization of cells and biological specimens for analysis |
US20080099715A1 (en) | 2004-03-23 | 2008-05-01 | Commonwealth Scientific And Industrial Research Or | Polymer Beads Incorporating Iron Oxide Particles |
CA2505464C (en) | 2004-04-28 | 2013-12-10 | Sunnybrook And Women's College Health Sciences Centre | Catheter tracking with phase information |
JP2005315677A (ja) * | 2004-04-28 | 2005-11-10 | Canon Inc | 検出装置および検出方法 |
FR2869996B1 (fr) * | 2004-05-05 | 2006-12-15 | Diagast Soc Par Actions Simpli | Utilisation de ferrofluides pour le phenotypage sanguin et applications derivees |
US7553132B2 (en) | 2004-05-20 | 2009-06-30 | Wisconsin Alumni Research Foundation | Micro device incorporating programmable element |
US20060019096A1 (en) * | 2004-06-01 | 2006-01-26 | Hatton T A | Field-responsive superparamagnetic composite nanofibers and methods of use thereof |
US7202667B2 (en) | 2004-06-07 | 2007-04-10 | California Institute Of Technology | Anisotropic nanoparticle amplification of magnetic resonance signals |
US7271592B1 (en) | 2004-06-14 | 2007-09-18 | U.S. Department Of Energy | Toroid cavity/coil NMR multi-detector |
US7754444B2 (en) | 2004-06-24 | 2010-07-13 | The Hong Kong University Of Science And Technology | Biofunctional magnetic nanoparticles for pathogen detection |
AU2005329068A1 (en) | 2004-07-29 | 2006-09-21 | Kim Laboratories, Inc. | Ultrasensitive sensor and rapid detection of analytes |
US8189899B2 (en) | 2004-07-30 | 2012-05-29 | Veridex, Llc | Methods and algorithms for cell enumeration in a low-cost cytometer |
WO2006015379A2 (en) | 2004-08-02 | 2006-02-09 | Cornell Research Foundation, Inc. | Electron spin resonance microscope for imaging with micron resolution |
CN102759466A (zh) | 2004-09-15 | 2012-10-31 | 英特基因有限公司 | 微流体装置 |
JP5165383B2 (ja) | 2004-12-23 | 2013-03-21 | アイ−スタツト・コーポレイシヨン | 分子診断システム及び方法 |
US7699979B2 (en) | 2005-01-07 | 2010-04-20 | Board Of Trustees Of The University Of Arkansas | Separation system and efficient capture of contaminants using magnetic nanoparticles |
US20080135490A1 (en) | 2005-01-07 | 2008-06-12 | Board Of Trustees Of The University Of Arkansas | Quantum dot biolabeling and immunomagnetic separation for detection of contaminants |
US7393665B2 (en) | 2005-02-10 | 2008-07-01 | Population Genetics Technologies Ltd | Methods and compositions for tagging and identifying polynucleotides |
WO2007075179A2 (en) | 2005-02-18 | 2007-07-05 | The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Bacteriophage-quantum dot (phage-qd) nanocomplex to detect biological targets in clinical and environmental isolates |
JP4791867B2 (ja) * | 2005-03-31 | 2011-10-12 | 日立マクセル株式会社 | 貴金属コート磁性粒子を用いた被検物質の検出方法 |
US7300631B2 (en) * | 2005-05-02 | 2007-11-27 | Bioscale, Inc. | Method and apparatus for detection of analyte using a flexural plate wave device and magnetic particles |
US20070037173A1 (en) | 2005-08-12 | 2007-02-15 | Allard Jeffrey W | Circulating tumor cells (CTC's): early assessment of time to progression, survival and response to therapy in metastatic cancer patients |
US20070031857A1 (en) | 2005-08-02 | 2007-02-08 | Rubicon Genomics, Inc. | Compositions and methods for processing and amplification of DNA, including using multiple enzymes in a single reaction |
US7901950B2 (en) | 2005-08-12 | 2011-03-08 | Veridex, Llc | Method for assessing disease states by profile analysis of isolated circulating endothelial cells |
EP1932009B1 (en) | 2005-08-31 | 2014-02-12 | T2 Biosystems, Inc. | Nmr device for detection of analytes involving magnetic particles |
JP2007097551A (ja) * | 2005-10-07 | 2007-04-19 | Daikin Ind Ltd | 微生物検出方法 |
US7763453B2 (en) * | 2005-11-30 | 2010-07-27 | Micronics, Inc. | Microfluidic mixing and analytic apparatus |
US20080241909A1 (en) * | 2007-03-27 | 2008-10-02 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Microfluidic chips for pathogen detection |
WO2007075891A2 (en) | 2005-12-23 | 2007-07-05 | Perkinelmer Las, Inc. | Multiplex assays using magnetic and non-magnetic particles |
US7274191B2 (en) | 2005-12-29 | 2007-09-25 | Intel Corporation | Integrated on-chip NMR and ESR device and method for making and using the same |
US7345479B2 (en) | 2005-12-29 | 2008-03-18 | Intel Corporation | Portable NMR device and method for making and using the same |
US7544473B2 (en) | 2006-01-23 | 2009-06-09 | Population Genetics Technologies Ltd. | Nucleic acid analysis using sequence tokens |
WO2007087312A2 (en) | 2006-01-23 | 2007-08-02 | Population Genetics Technologies Ltd. | Molecular counting |
US20090227044A1 (en) * | 2006-01-26 | 2009-09-10 | Dosi Dosev | Microchannel Magneto-Immunoassay |
US7932099B2 (en) | 2006-02-21 | 2011-04-26 | Nexus Dx, Inc. | Methods and compositions for analyte detection |
JP5254949B2 (ja) | 2006-03-15 | 2013-08-07 | マイクロニクス, インコーポレイテッド | 一体型の核酸アッセイ |
US8945946B2 (en) | 2006-03-31 | 2015-02-03 | Canon Kabushiki Kaisha | Sensor element and detection method of magnetic particles using this element, and detection method of target substance |
US7282337B1 (en) | 2006-04-14 | 2007-10-16 | Helicos Biosciences Corporation | Methods for increasing accuracy of nucleic acid sequencing |
WO2007123342A1 (en) | 2006-04-20 | 2007-11-01 | Korea Institute Of Constructuion Technology | The linear infiltration system functioning as a storm sewer |
WO2007123345A1 (en) | 2006-04-21 | 2007-11-01 | Peoplebio, Inc. | Method for differentially detecting multimeric form from monomeric form of multimer-forming polypeptides through three-dimensional interactions |
US8007999B2 (en) | 2006-05-10 | 2011-08-30 | Theranos, Inc. | Real-time detection of influenza virus |
KR20090031362A (ko) | 2006-05-18 | 2009-03-25 | 페터리내르메디찌니쉐 우니버지태트 빈 | 인플루엔자 바이러스의 검출 방법 |
US20080026451A1 (en) | 2006-06-15 | 2008-01-31 | Braman Jeffrey C | System for isolating biomolecules from a sample |
AU2007285775B2 (en) * | 2006-08-17 | 2011-03-03 | Sanofi Pasteur Ltd. | Immunogenic PcpA polypeptides and uses thereof |
GB0616508D0 (en) * | 2006-08-18 | 2006-09-27 | Iti Scotland Ltd | Analyte manipulation and detection |
US7405567B2 (en) | 2006-08-21 | 2008-07-29 | Abqmr, Inc. | Tuning low-inductance coils at low frequencies |
US8339135B2 (en) | 2006-08-21 | 2012-12-25 | Stc.Unm | Biological detector and method |
US20090256572A1 (en) | 2008-04-14 | 2009-10-15 | Mcdowell Andrew F | Tuning Low-Inductance Coils at Low Frequencies |
US20080081330A1 (en) | 2006-09-28 | 2008-04-03 | Helicos Biosciences Corporation | Method and devices for analyzing small RNA molecules |
US8368402B2 (en) | 2006-11-08 | 2013-02-05 | T2 Biosystems, Inc. | NMR systems for in vivo detection of analytes |
US20080113350A1 (en) | 2006-11-09 | 2008-05-15 | Terstappen Leon W M M | Blood test to monitor the genetic changes of progressive cancer using immunomagnetic enrichment and fluorescence in situ hybridization (FISH) |
US8262900B2 (en) | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
ES2923759T3 (es) | 2006-12-14 | 2022-09-30 | Life Technologies Corp | Aparato para medir analitos utilizando matrices de FET |
US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
US7820454B2 (en) * | 2006-12-29 | 2010-10-26 | Intel Corporation | Programmable electromagnetic array for molecule transport |
US8354514B2 (en) | 2007-01-12 | 2013-01-15 | Lawrence Livermore National Security, Llc | Multiplex detection of agricultural pathogens |
JP4458103B2 (ja) | 2007-02-27 | 2010-04-28 | Tdk株式会社 | 磁気センサ、磁気方位センサ、磁界検出方法および磁気方位検出方法 |
MX2009010221A (es) | 2007-03-23 | 2009-10-19 | Wyeth Corp | Proceso abreviado de purificacion para la produccion de polisacaridos capsulares de streptococcus pneumoniae. |
WO2008119054A1 (en) | 2007-03-27 | 2008-10-02 | Abqmr, Inc. | System and method for detecting labeled entities using microcoil magnetic mri |
WO2009008925A2 (en) * | 2007-04-05 | 2009-01-15 | The Regents Of The University Of California | A particle-based microfluidic device for providing high magnetic field gradients |
US20100144005A1 (en) | 2007-04-26 | 2010-06-10 | Bin Kingombe Cesar G I | Process for isolating microorganisms from samples and system, apparatus and compositions therefor |
US8067938B2 (en) | 2007-05-03 | 2011-11-29 | Abqmr, Inc. | Microcoil NMR detectors |
EP1992938A1 (en) | 2007-05-14 | 2008-11-19 | Koninklijke Philips Electronics N.V. | Improved methods of SE(R)RS detection using multiple labels |
ATE554859T1 (de) | 2007-05-24 | 2012-05-15 | Univ California | Integrierte fluidische vorrichtungen mit magnetischer sortierung |
WO2009048673A2 (en) | 2007-07-26 | 2009-04-16 | University Of Chicago | Stochastic confinement to detect, manipulate, and utilize molecules and organisms |
JP2010536565A (ja) | 2007-08-23 | 2010-12-02 | シンベニオ・バイオシステムズ・インコーポレーテッド | 目標種用のトラップ用磁気選別システム |
US8110101B2 (en) | 2007-08-30 | 2012-02-07 | Veridex, Llc | Method and apparatus for imaging target components in a biological sample using permanent magnets |
US7828968B2 (en) | 2007-08-30 | 2010-11-09 | Veridex, Llc | Method and apparatus for imaging target components in a biological sample using permanent magnets |
US20090061456A1 (en) | 2007-08-30 | 2009-03-05 | Allard William J | Method for predicting progression free and overall survival at each follow-up time point during therapy of metastatic breast cancer patients using circulating tumor cells |
US8709727B2 (en) | 2007-09-17 | 2014-04-29 | Mattias Strömberg | Magnetic detection of small entities |
US20110091987A1 (en) | 2007-10-04 | 2011-04-21 | Ralph Weissleder | Miniaturized Magnetic Resonance Systems and Methods |
WO2009055587A1 (en) | 2007-10-23 | 2009-04-30 | Abqmr, Inc. | Microcoil magnetic resonance detectors |
CN101878429B (zh) | 2007-11-27 | 2015-05-20 | 维里德克斯有限责任公司 | 血液中循环黑素瘤细胞的自动化计数和表征 |
TW200930713A (en) | 2007-12-03 | 2009-07-16 | Takeda Pharmaceutical | Nitrogen-containing heterocyclic compound and use thereof |
NO2227271T3 (ja) * | 2007-12-07 | 2018-06-23 | ||
EP2229441B1 (en) | 2007-12-12 | 2014-10-01 | The Board of Trustees of The Leland Stanford Junior University | Method and apparatus for magnetic separation of cells |
US20090191535A1 (en) | 2007-12-22 | 2009-07-30 | Mark Carle Connelly | Method of assessing metastatic carcinomas from circulating endothelial cells and disseminated tumor cells |
EP2239575A4 (en) * | 2007-12-28 | 2013-01-02 | Sysmex Corp | REAGENT FOR DETECTING HIV-1 ANTIGEN AND DETECTION METHOD |
CN101952727A (zh) | 2007-12-31 | 2011-01-19 | 3M创新有限公司 | 微生物捕获用组合物和方法 |
US8372590B2 (en) | 2008-01-07 | 2013-02-12 | Luminex Corporation | Isolation and enumeration of cells from a complex sample matrix |
US20090258365A1 (en) | 2008-03-25 | 2009-10-15 | Terstappen Leon W M M | METHOD FOR DETECTING IGF1R/Chr 15 in CIRCULATING TUMOR CELLS USING FISH |
GB0806147D0 (en) | 2008-04-04 | 2008-05-14 | Alaska Food Diagnostics | Assay system |
WO2009129415A1 (en) | 2008-04-16 | 2009-10-22 | Cynvenio Biosystems, Llc | Magnetic separation system with pre and post processing modules |
US8569077B2 (en) | 2008-05-19 | 2013-10-29 | Veridex, Llc | Imaging of immunomagnetically enriched rare cells |
EP2303463A4 (en) | 2008-06-17 | 2013-12-04 | Georgia Tech Res Inst | SUPERPARAMAGNETIC NANOPARTICLES FOR THE ELIMINATION OF CELLS, PATHOGENS OR VIRUSES |
KR101005924B1 (ko) | 2008-06-27 | 2011-01-06 | 포항공과대학교 산학협력단 | 핵산 추출 장치 |
US8053250B2 (en) * | 2008-06-27 | 2011-11-08 | Rex Chin-Yih Hong | Method and system for suppressing bindings on magnetic particles |
US20100184033A1 (en) | 2008-07-16 | 2010-07-22 | West Michael D | Methods to accelerate the isolation of novel cell strains from pluripotent stem cells and cells obtained thereby |
US20100035252A1 (en) | 2008-08-08 | 2010-02-11 | Ion Torrent Systems Incorporated | Methods for sequencing individual nucleic acids under tension |
AU2009296662B2 (en) | 2008-09-24 | 2016-08-11 | First Light Diagnostics, Inc. | Kits and devices for detecting analytes |
CN102187241B (zh) * | 2008-10-17 | 2015-11-25 | 皇家飞利浦电子股份有限公司 | 用于灵敏测定的脉冲磁激励 |
US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
US20100301398A1 (en) | 2009-05-29 | 2010-12-02 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
US8546128B2 (en) | 2008-10-22 | 2013-10-01 | Life Technologies Corporation | Fluidics system for sequential delivery of reagents |
WO2010056728A1 (en) | 2008-11-11 | 2010-05-20 | Helicos Biosciences Corporation | Nucleic acid encoding for multiplex analysis |
US20100129785A1 (en) * | 2008-11-21 | 2010-05-27 | General Electric Company | Agents and methods for spectrometric analysis |
WO2010123594A2 (en) * | 2009-01-15 | 2010-10-28 | Children's Medical Center Corporation | Device for filtration of fluids there through and accompanying method |
FR2945126B1 (fr) * | 2009-04-29 | 2019-11-01 | Commissariat A L'energie Atomique | Procede et appareil de comptage des thrombocytes |
US20100282788A1 (en) | 2009-05-06 | 2010-11-11 | Liberti Paul A | Asymmetric hanger for short and long trousers |
US8574835B2 (en) | 2009-05-29 | 2013-11-05 | Life Technologies Corporation | Scaffolded nucleic acid polymer particles and methods of making and using |
US8673627B2 (en) | 2009-05-29 | 2014-03-18 | Life Technologies Corporation | Apparatus and methods for performing electrochemical reactions |
EP2464753A4 (en) | 2009-08-12 | 2013-12-11 | Harvard College | BIO RECOGNITION METHOD AND COMPOSITIONS |
US20110046475A1 (en) | 2009-08-24 | 2011-02-24 | Benny Assif | Techniques for correcting temperature measurement in magnetic resonance thermometry |
JP2011177092A (ja) * | 2010-02-26 | 2011-09-15 | Canon Inc | リガンドのスクリーニング方法 |
US20110262989A1 (en) | 2010-04-21 | 2011-10-27 | Nanomr, Inc. | Isolating a target analyte from a body fluid |
US9428547B2 (en) | 2010-04-21 | 2016-08-30 | Dna Electronics, Inc. | Compositions for isolating a target analyte from a heterogeneous sample |
US8841104B2 (en) | 2010-04-21 | 2014-09-23 | Nanomr, Inc. | Methods for isolating a target analyte from a heterogeneous sample |
US20140100136A1 (en) | 2010-04-21 | 2014-04-10 | Nanomr, Inc. | Isolation and characterization of pathogens |
US9476812B2 (en) | 2010-04-21 | 2016-10-25 | Dna Electronics, Inc. | Methods for isolating a target analyte from a heterogeneous sample |
US20130109590A1 (en) | 2010-04-21 | 2013-05-02 | Lisa-Jo Ann CLARIZIA | Isolating a target analyte from a body fluid |
TWI539158B (zh) | 2010-06-08 | 2016-06-21 | 維里德克斯有限責任公司 | 使用血液中之循環黑色素瘤細胞預測黑色素瘤病患之臨床結果的方法。 |
US20130196341A1 (en) | 2010-07-06 | 2013-08-01 | T2 Biosystems ,Inc. | Methods and compositions for detection of analytes |
WO2012024658A2 (en) | 2010-08-20 | 2012-02-23 | IntegenX, Inc. | Integrated analysis system |
BR112013009008A2 (pt) | 2010-10-14 | 2017-10-17 | Veridex Llc | métodos e kits para a detecção de células circulantes de tumor em pacientes pancreáticos com o uso de reagentes de detecção de captura e de coquetel poliespecíficos |
US8563298B2 (en) | 2010-10-22 | 2013-10-22 | T2 Biosystems, Inc. | NMR systems and methods for the rapid detection of analytes |
US20140170727A1 (en) | 2012-12-19 | 2014-06-19 | Nanomr, Inc. | Affinity medium using fixed whole cells |
US9434940B2 (en) | 2012-12-19 | 2016-09-06 | Dna Electronics, Inc. | Methods for universal target capture |
US9599610B2 (en) | 2012-12-19 | 2017-03-21 | Dnae Group Holdings Limited | Target capture system |
US20140170667A1 (en) | 2012-12-19 | 2014-06-19 | Nanomr, Inc. | Methods for amplifying nucleic acid from a target |
US9551704B2 (en) | 2012-12-19 | 2017-01-24 | Dna Electronics, Inc. | Target detection |
US9804069B2 (en) | 2012-12-19 | 2017-10-31 | Dnae Group Holdings Limited | Methods for degrading nucleic acid |
US9995742B2 (en) | 2012-12-19 | 2018-06-12 | Dnae Group Holdings Limited | Sample entry |
US10000557B2 (en) | 2012-12-19 | 2018-06-19 | Dnae Group Holdings Limited | Methods for raising antibodies |
US20140170669A1 (en) | 2012-12-19 | 2014-06-19 | Nanomr, Inc. | Devices for target detection and methods of use thereof |
-
2010
- 2010-08-04 US US12/850,203 patent/US20110262989A1/en not_active Abandoned
- 2010-08-12 US US12/855,147 patent/US9389225B2/en active Active
-
2011
- 2011-04-20 JP JP2013506262A patent/JP5814344B2/ja active Active
- 2011-04-20 WO PCT/US2011/033184 patent/WO2011133630A1/en active Application Filing
- 2011-04-20 WO PCT/US2011/033186 patent/WO2011133632A1/en active Application Filing
- 2011-04-20 EP EP11772606.7A patent/EP2561360B9/en active Active
- 2011-04-20 CA CA2796767A patent/CA2796767C/en active Active
- 2011-04-20 ES ES16190239T patent/ES2774393T3/es active Active
- 2011-04-20 EP EP16190239.0A patent/EP3138927B1/en active Active
- 2011-04-20 CA CA2796897A patent/CA2796897C/en active Active
- 2011-04-20 ES ES11772606.7T patent/ES2656965T3/es active Active
- 2011-04-20 EP EP11772608.3A patent/EP2561099B1/en active Active
- 2011-04-21 US US13/091,527 patent/US20110262933A1/en not_active Abandoned
- 2011-04-21 JP JP2013506301A patent/JP6023698B2/ja active Active
- 2011-04-21 US US13/091,548 patent/US9671395B2/en active Active
- 2011-04-21 ES ES11772698.4T patent/ES2685471T3/es active Active
- 2011-04-21 US US13/091,534 patent/US9562896B2/en active Active
- 2011-04-21 CA CA2796900A patent/CA2796900C/en active Active
- 2011-04-21 WO PCT/US2011/033410 patent/WO2011133759A1/en active Application Filing
- 2011-04-21 US US13/091,518 patent/US20110262932A1/en not_active Abandoned
- 2011-04-21 EP EP11772699.2A patent/EP2561361A4/en not_active Ceased
- 2011-04-21 EP EP11772698.4A patent/EP2561354B1/en active Active
- 2011-04-21 CA CA2796912A patent/CA2796912A1/en not_active Abandoned
- 2011-04-21 WO PCT/US2011/033411 patent/WO2011133760A1/en active Application Filing
-
2016
- 2016-07-11 US US15/206,751 patent/US11073513B2/en active Active
- 2016-10-07 JP JP2016198681A patent/JP2017012198A/ja active Pending
-
2017
- 2017-02-06 US US15/425,539 patent/US20170145406A1/en not_active Abandoned
- 2017-04-25 US US15/496,790 patent/US9869671B2/en active Active
-
2018
- 2018-01-10 US US15/866,628 patent/US10677789B2/en active Active
- 2018-01-12 US US15/869,146 patent/US11448646B2/en active Active
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US11448646B2 (en) | Isolating a target analyte from a body fluid | |
US9863940B2 (en) | Methods for isolating a target analyte from a heterogenous sample | |
US9428547B2 (en) | Compositions for isolating a target analyte from a heterogeneous sample | |
US20130109590A1 (en) | Isolating a target analyte from a body fluid |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20140421 |
|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20140421 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20150626 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20150925 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20151026 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20160308 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20160607 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20160615 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20160907 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20161007 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 6023698 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313113 |
|
S531 | Written request for registration of change of domicile |
Free format text: JAPANESE INTERMEDIATE CODE: R313531 |
|
S533 | Written request for registration of change of name |
Free format text: JAPANESE INTERMEDIATE CODE: R313533 |
|
R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |