BR112013009008A2 - métodos e kits para a detecção de células circulantes de tumor em pacientes pancreáticos com o uso de reagentes de detecção de captura e de coquetel poliespecíficos - Google Patents

métodos e kits para a detecção de células circulantes de tumor em pacientes pancreáticos com o uso de reagentes de detecção de captura e de coquetel poliespecíficos

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Publication number
BR112013009008A2
BR112013009008A2 BR112013009008A BR112013009008A BR112013009008A2 BR 112013009008 A2 BR112013009008 A2 BR 112013009008A2 BR 112013009008 A BR112013009008 A BR 112013009008A BR 112013009008 A BR112013009008 A BR 112013009008A BR 112013009008 A2 BR112013009008 A2 BR 112013009008A2
Authority
BR
Brazil
Prior art keywords
capture
ferrofluid
polyspecific
kits
methods
Prior art date
Application number
BR112013009008A
Other languages
English (en)
Inventor
Chandra Rao Galla
Carle Connelly Mark
Original Assignee
Veridex Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Veridex Llc filed Critical Veridex Llc
Publication of BR112013009008A2 publication Critical patent/BR112013009008A2/pt

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G01N33/57492—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds localized on the membrane of tumor or cancer cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

métodos e kits para a detecção de células circulantes de tumor em pacientes pancreáticos com o uso de reagentes de detecção de captura e de coquetel poliespecificos. a presente invenção refere-se a um teste altamente sensível que combina enriquecimento imuno-magnético com citometria de fluxo multiparamétrica ou citometria de imagem para detectar, enumerar e caracterizar células de carcinoma no sangue. a presente invenção incorpora a conjugação de diferentes anticorpos ao mesmo ferrofluido. isso tem o efeito de produzir o ferrofluido poliespecífico que diz respeito aos antígenos que o ferrofluido irá ligar-se. os múltiplos anticorpos presentes no mesmo ferrofluido não aparecem bloquear ou de outro modo interferir uns com os outros. tais ferrofluidos têm o efeito altamente desejável de ser capaz de ligar-se especificamente a mais de um tipo de célula. o teste é especialmente útil para possibilitar a captura de ctcs que têm baixa expressão de epcam, mas alta expressão de outros marcadores de tumor; consequentemente, o teste facilita a caracterização biológica e estadiamento das células de carcinoma.
BR112013009008A 2010-10-14 2011-10-13 métodos e kits para a detecção de células circulantes de tumor em pacientes pancreáticos com o uso de reagentes de detecção de captura e de coquetel poliespecíficos BR112013009008A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39303610P 2010-10-14 2010-10-14
PCT/US2011/056100 WO2012051390A1 (en) 2010-10-14 2011-10-13 Methods and kits for the detection of circulating tumor cells in pancreatic patients using polyspecific capture and cocktail detection reagents

Publications (1)

Publication Number Publication Date
BR112013009008A2 true BR112013009008A2 (pt) 2017-10-17

Family

ID=44906390

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013009008A BR112013009008A2 (pt) 2010-10-14 2011-10-13 métodos e kits para a detecção de células circulantes de tumor em pacientes pancreáticos com o uso de reagentes de detecção de captura e de coquetel poliespecíficos

Country Status (12)

Country Link
US (1) US20120094275A1 (pt)
EP (1) EP2628008B1 (pt)
JP (1) JP5960146B2 (pt)
KR (1) KR20130119933A (pt)
CN (1) CN103154740B (pt)
BR (1) BR112013009008A2 (pt)
CA (1) CA2814528A1 (pt)
ES (1) ES2634220T3 (pt)
IL (1) IL225646A0 (pt)
MX (1) MX347964B (pt)
TW (1) TWI577389B (pt)
WO (1) WO2012051390A1 (pt)

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Also Published As

Publication number Publication date
TWI577389B (zh) 2017-04-11
IL225646A0 (en) 2013-06-27
CN103154740B (zh) 2016-08-03
JP2013539868A (ja) 2013-10-28
WO2012051390A1 (en) 2012-04-19
US20120094275A1 (en) 2012-04-19
CA2814528A1 (en) 2012-04-19
MX347964B (es) 2017-05-18
CN103154740A (zh) 2013-06-12
EP2628008B1 (en) 2017-05-31
JP5960146B2 (ja) 2016-08-02
KR20130119933A (ko) 2013-11-01
ES2634220T3 (es) 2017-09-27
TW201225978A (en) 2012-07-01
EP2628008A1 (en) 2013-08-21
MX2013004172A (es) 2013-12-12

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