MX347964B - Metodos y kits para la deteccion de células tumorales circulantes en pacientes pancreáticos por medio del uso de captura poliespecífica y reactivos de detección de cocteles. - Google Patents

Metodos y kits para la deteccion de células tumorales circulantes en pacientes pancreáticos por medio del uso de captura poliespecífica y reactivos de detección de cocteles.

Info

Publication number
MX347964B
MX347964B MX2013004172A MX2013004172A MX347964B MX 347964 B MX347964 B MX 347964B MX 2013004172 A MX2013004172 A MX 2013004172A MX 2013004172 A MX2013004172 A MX 2013004172A MX 347964 B MX347964 B MX 347964B
Authority
MX
Mexico
Prior art keywords
ferrofluid
detection
polyspecific
capture
assay
Prior art date
Application number
MX2013004172A
Other languages
English (en)
Other versions
MX2013004172A (es
Inventor
Mark Carle Connelly
Galla Chandra Rao
Original Assignee
Veridex Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Veridex Llc filed Critical Veridex Llc
Publication of MX2013004172A publication Critical patent/MX2013004172A/es
Publication of MX347964B publication Critical patent/MX347964B/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G01N33/57492—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds localized on the membrane of tumor or cancer cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction

Abstract

La presente invención se refiere a un ensayo altamente sensible que combina enriquecimiento inmunomagnético con citometría multiparamétrica o citometría de imágenes para detectar, enumerar y caracterizar células carcinomas en la sangre; la presente invención incorpora la conjugación de distintos anticuerpos al mismo ferrofluido; esto tiene el efecto de hacer al ferrofluido poliespecífico con respecto a los antígenos que el ferrofluido va a unir; los múltiples anticuerpos presentes en el mismo ferrofluido no parecen bloquearse o, de cualquiera otra forma, obstaculizarse entre sí; estos ferrofluidos tienen el muy deseable efecto de poder unirse específicamente a más de un tipo de célula; el ensayo es especialmente útil para permitir la captura de las CTC que tienen baja expresión de EpCAM, pero alta expresión de otros marcadores de tumores; por consiguiente, el ensayo facilita la caracterización biológica y la estadificación de células carcinomas. Figura 1B.
MX2013004172A 2010-10-14 2011-10-13 Metodos y kits para la deteccion de células tumorales circulantes en pacientes pancreáticos por medio del uso de captura poliespecífica y reactivos de detección de cocteles. MX347964B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39303610P 2010-10-14 2010-10-14
PCT/US2011/056100 WO2012051390A1 (en) 2010-10-14 2011-10-13 Methods and kits for the detection of circulating tumor cells in pancreatic patients using polyspecific capture and cocktail detection reagents

Publications (2)

Publication Number Publication Date
MX2013004172A MX2013004172A (es) 2013-12-12
MX347964B true MX347964B (es) 2017-05-18

Family

ID=44906390

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013004172A MX347964B (es) 2010-10-14 2011-10-13 Metodos y kits para la deteccion de células tumorales circulantes en pacientes pancreáticos por medio del uso de captura poliespecífica y reactivos de detección de cocteles.

Country Status (12)

Country Link
US (1) US20120094275A1 (es)
EP (1) EP2628008B1 (es)
JP (1) JP5960146B2 (es)
KR (1) KR20130119933A (es)
CN (1) CN103154740B (es)
BR (1) BR112013009008A2 (es)
CA (1) CA2814528A1 (es)
ES (1) ES2634220T3 (es)
IL (1) IL225646A0 (es)
MX (1) MX347964B (es)
TW (1) TWI577389B (es)
WO (1) WO2012051390A1 (es)

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Also Published As

Publication number Publication date
CN103154740B (zh) 2016-08-03
IL225646A0 (en) 2013-06-27
CN103154740A (zh) 2013-06-12
US20120094275A1 (en) 2012-04-19
TWI577389B (zh) 2017-04-11
JP5960146B2 (ja) 2016-08-02
BR112013009008A2 (pt) 2017-10-17
JP2013539868A (ja) 2013-10-28
CA2814528A1 (en) 2012-04-19
EP2628008B1 (en) 2017-05-31
EP2628008A1 (en) 2013-08-21
TW201225978A (en) 2012-07-01
WO2012051390A1 (en) 2012-04-19
KR20130119933A (ko) 2013-11-01
MX2013004172A (es) 2013-12-12
ES2634220T3 (es) 2017-09-27

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