AR103745A1 - Inmunoensayo homogéneo con compensación de la señal de fondo - Google Patents

Inmunoensayo homogéneo con compensación de la señal de fondo

Info

Publication number
AR103745A1
AR103745A1 ARP160100454A ARP160100454A AR103745A1 AR 103745 A1 AR103745 A1 AR 103745A1 AR P160100454 A ARP160100454 A AR P160100454A AR P160100454 A ARP160100454 A AR P160100454A AR 103745 A1 AR103745 A1 AR 103745A1
Authority
AR
Argentina
Prior art keywords
compensation
immunoensay
homogenous
fund
signal
Prior art date
Application number
ARP160100454A
Other languages
English (en)
Original Assignee
Idexx Lab Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=56689082&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AR103745(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Idexx Lab Inc filed Critical Idexx Lab Inc
Publication of AR103745A1 publication Critical patent/AR103745A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01049—Glucose-6-phosphate dehydrogenase (1.1.1.49)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6806—Determination of free amino acids
    • G01N33/6812—Assays for specific amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/34—Genitourinary disorders
    • G01N2800/347—Renal failures; Glomerular diseases; Tubulointerstitial diseases, e.g. nephritic syndrome, glomerulonephritis; Renovascular diseases, e.g. renal artery occlusion, nephropathy

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Steroid Compounds (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Los inmunoensayos homogéneos que permiten la compensación de señales de fondo en muestras y reactivos. El uso de inmunoensayos homogéneos para detectar la presencia o la cantidad de dimetil arginina simétrica (SDMA) en muestras biológicas. Reactivos y juegos para realizar los ensayos.
ARP160100454A 2015-02-20 2016-02-23 Inmunoensayo homogéneo con compensación de la señal de fondo AR103745A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201562118832P 2015-02-20 2015-02-20

Publications (1)

Publication Number Publication Date
AR103745A1 true AR103745A1 (es) 2017-05-31

Family

ID=56689082

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP160100454A AR103745A1 (es) 2015-02-20 2016-02-23 Inmunoensayo homogéneo con compensación de la señal de fondo

Country Status (16)

Country Link
US (2) US10775365B2 (es)
EP (2) EP4170346A1 (es)
JP (2) JP6896637B2 (es)
KR (2) KR102492243B1 (es)
CN (2) CN113699125A (es)
AR (1) AR103745A1 (es)
AU (2) AU2016219847B2 (es)
BR (1) BR112017017719A2 (es)
CA (1) CA2977062A1 (es)
ES (1) ES2936294T3 (es)
FI (1) FI3259593T3 (es)
HU (1) HUE060884T2 (es)
MX (1) MX2017010505A (es)
PL (1) PL3259593T3 (es)
TW (1) TWI721966B (es)
WO (1) WO2016134251A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102492243B1 (ko) 2015-02-20 2023-01-25 아이덱스 래보러토리즈, 인코포레이티드 배경 신호에 대한 보상에 의한 균질 면역검정
KR20200073259A (ko) * 2017-10-19 2020-06-23 아이덱스 래보러토리즈, 인코포레이티드 대칭 디메틸아르기닌의 검출
US10745492B1 (en) * 2019-04-03 2020-08-18 Ark Diagnostics, Inc. Antibodies to symmetrically dimethylated arginine analytes and use thereof

Family Cites Families (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3875011A (en) 1972-11-06 1975-04-01 Syva Co Enzyme immunoassays with glucose-6-phosphate dehydrogenase
US4341866A (en) * 1980-06-02 1982-07-27 Syva Company Antienzyme termination in enzyme immunoassays
DE3134787A1 (de) 1981-09-02 1983-03-10 Boehringer Mannheim Gmbh, 6800 Mannheim Creatinin-antikoerper
IT1199088B (it) * 1984-03-09 1988-12-30 Miles Italiana Saggio di legame specifico mediante impiego di anti-g6pdh come marcante
US4818703A (en) 1985-10-23 1989-04-04 Pizzolante John M Stabilized alkaline picrate reagent for jaffe creatinine determination
US6461825B1 (en) * 1987-09-30 2002-10-08 Sanofi (Societe Anonyme) Immunometric assay kit and method applicable to whole cells
EP0356160A3 (en) 1988-08-24 1991-09-11 The Board Of Trustees Of The Leland Stanford Junior University Capillary device
EP0399127A1 (en) * 1989-05-23 1990-11-28 Pharmacia ENI Diagnostics Inc. Homogeneous immunochemical method for determining haptens by means of ion selective electrodes
US5726010A (en) 1991-07-31 1998-03-10 Idexx Laboratories, Inc. Reversible flow chromatographic binding assay
US6455288B1 (en) * 1993-04-08 2002-09-24 Dade Behring Marburg Gmbh Homogeneous immunoassays using mutant glucose-6-phosphate dehydrogenases
US5972703A (en) * 1994-08-12 1999-10-26 The Regents Of The University Of Michigan Bone precursor cells: compositions and methods
US5804452A (en) 1995-04-27 1998-09-08 Quidel Corporation One step urine creatinine assays
WO1998049199A1 (en) 1997-05-01 1998-11-05 Panorama Research, Inc. Cardiovascular disease risk assessment
US6736957B1 (en) 1997-10-16 2004-05-18 Abbott Laboratories Biosensor electrode mediators for regeneration of cofactors and process for using
US6358699B1 (en) 1999-02-05 2002-03-19 Cooke Pharma Assay for asymmetrical NG, NG dimethyl-l-arginine
WO2001046221A1 (en) 1999-12-22 2001-06-28 The Research Foundation Of State University Of New York Protein methylarginine-specific antibodies
WO2002004465A1 (en) 2000-07-06 2002-01-17 Fal Diagnostics Methods and kits for the detection of arginine compounds
EP1301790B1 (en) 2000-07-12 2008-04-09 Naser, Werner, Dr. Direct assessment of analyte to reference molecule ratios
DE10040904A1 (de) 2000-08-18 2002-02-28 Boeger Rainer H Verfahren und Mittel zum Nachweis einer Wahrscheinlichkeit zukünftigen Fortschreitens von Gefässerkrankungen
US6601006B2 (en) * 2000-12-22 2003-07-29 Idexx Laboratories, Inc. Methods for the calibration of analyte assays
US7241856B2 (en) 2003-06-02 2007-07-10 Pentron Clinical Technologies Llc Dental resins, dental composite materials, and method of manufacture thereof
FR2824825B1 (fr) * 2001-05-15 2005-05-06 Servier Lab Nouveaux derives d'alpha-amino-acides, leur procede de preparation et les compositions pharmaceutiques qui les contiennent
JP4214271B2 (ja) 2002-10-15 2009-01-28 アークレイ株式会社 クレアチニン測定用試験片
US7501053B2 (en) 2002-10-23 2009-03-10 Abbott Laboratories Biosensor having improved hematocrit and oxygen biases
WO2004046314A2 (en) 2002-11-15 2004-06-03 The Board Of Trustees Of The Leland Stanford Junior University Methods for detecting asymmetric dimethylarginine in a biological sample
US20050148029A1 (en) 2003-09-29 2005-07-07 Biosite, Inc. Methods and compositions for determining treatment regimens in systemic inflammatory response syndromes
US6991911B2 (en) 2003-12-15 2006-01-31 Dade Behring Inc. Assay for entactogens
US7813880B2 (en) 2004-03-25 2010-10-12 University Of Maryland, Baltimore Apparatus and method for providing optimal concentrations for medication infusions
CN102768273B (zh) 2004-03-30 2015-08-26 通用电气医疗集团生物科学公司 侧流格式、材料和方法
US7220842B2 (en) 2004-04-05 2007-05-22 Dade Behring Inc. Immunoassays for buprenorphine and norbuprenorphine
US20060046273A1 (en) * 2004-08-27 2006-03-02 Lin-Zhi International Inc. Homogeneous enzyme immunoassay for oral fluid
ES2332218T3 (es) 2004-11-04 2010-01-29 Germediq Forschungs- Und Entwicklungsgesellschaft Mbh Metodo para la determinacion de arginina, argininas metiladas y sus derivados.
CA2495138C (en) * 2005-01-20 2012-10-23 Alison Jane Basile Multiplexed analysis for determining a serodiagnosis of viral infection
WO2006078813A2 (en) 2005-01-21 2006-07-27 Biosite Incorporated Arginine analogs, and methods for their synthesis and use
US7723127B2 (en) 2005-03-03 2010-05-25 Novx Systems Inc. Immunoassay with extended dynamic range
CA2609702C (en) 2005-04-28 2013-05-28 Ventana Medical Systems, Inc. Antibody conjugates via heterobifunctional peg linkers
US8263342B2 (en) 2005-10-27 2012-09-11 Yale University Urinary proteomic biomarker patterns in preeclampsia
DE102005060057A1 (de) 2005-12-15 2007-06-28 Kellner, Karl-Heinz, Dr. Sandwich-Immunoassay für kleine Haptenmoleküle
JP2007176872A (ja) 2005-12-28 2007-07-12 Sentan Seimei Kagaku Kenkyusho:Kk アシンメトリックジメチルアルギニンを認識する抗体及びその製造方法並びに翻訳後修飾アミノ酸含有タンパク質の検出方法
CA2644499A1 (en) * 2006-03-02 2007-09-13 Perkinelmer Las, Inc. Methods for distinguishing isomers using mass spectrometry
WO2008154457A1 (en) 2007-06-08 2008-12-18 Ark Diagnosties, Inc. Topiramate immunoassays
FR2919063B1 (fr) 2007-07-19 2009-10-02 Biomerieux Sa Procede de dosage du leucocyte elastase inhibitor pour le diagnostic in vitro du cancer colorectal.
EP2229452B1 (en) 2007-12-05 2015-07-29 King's College London Method for aiding the diagnosis of a disorder
EP3808733A1 (en) * 2008-08-07 2021-04-21 IDEXX Laboratories, Inc. Methods for detecting symmetrical demethylarginine
WO2010048423A1 (en) 2008-10-24 2010-04-29 Ark Diagnostics, Inc. Levetiracetam immunoassays
ES2499415T3 (es) 2009-06-02 2014-09-29 Biocrates Life Sciences Ag Nuevos biomarcadores para la evaluación de enfermedades renales
CN101587118B (zh) 2009-07-03 2012-09-12 长春迪瑞医疗科技股份有限公司 肌酐尿液分析试纸及制备方法
CN101598727B (zh) 2009-07-09 2012-10-10 上海科华生物工程股份有限公司 定量测定人体血液尿素含量的干化学试纸
CN101865911A (zh) 2010-03-16 2010-10-20 苏州市玮琪生物科技有限公司 一种尿肌酐定量检测卡
US8329424B2 (en) * 2010-06-25 2012-12-11 Siemens Healthcare Diagnostics Reduction in false results in assay measurements
WO2012065912A1 (de) 2010-11-17 2012-05-24 Karl-Heinz Kellner Automatenfähiger immunoassay für biogene amine
JP2012112785A (ja) 2010-11-24 2012-06-14 Tohoku Univ 透析膜の評価方法
CN102154444B (zh) * 2010-12-30 2013-09-25 北京九强生物技术股份有限公司 测定不对称二甲基精氨酸浓度的方法及诊断试剂盒
US8394647B2 (en) 2011-02-17 2013-03-12 Siemens Healthcare Diagnostics Inc. Reducing non-covalently bound polysaccharide on supports
CN102628868B (zh) 2011-12-30 2014-09-17 北京九强生物技术股份有限公司 检测非对称二甲基精氨酸含量的胶乳增强免疫比浊法试剂盒
AU2013208289B2 (en) 2012-01-10 2016-08-11 Idexx Laboratories, Inc. Immunoassay test slide
WO2013124478A1 (en) * 2012-02-24 2013-08-29 Universität des Saarlandes Symmetric dimethylarginine (sdma) modifies high density lipoprotein (hdl) to induce endothelial dysfunction
US9074482B2 (en) * 2012-04-24 2015-07-07 United Technologies Corporation Airfoil support method and apparatus
US20140038203A1 (en) 2012-07-09 2014-02-06 Musc Foundation For Research Development Methods for detecting or predicting kidney disease
ES2822907T3 (es) * 2012-08-21 2021-05-05 Janssen Pharmaceutica Nv Haptenos de quetiapina para su uso en inmunoensayos
WO2014113717A1 (en) 2013-01-18 2014-07-24 Ark Diagnostics, Inc. Voriconazole immunoassays
EP2956444B1 (en) 2013-02-13 2018-05-30 ARK Diagnostics, Inc. Posaconazole immunoassays
WO2015035155A1 (en) 2013-09-05 2015-03-12 Idexx Laboratories, Inc. Methods for detecting renal disease
ES2911268T3 (es) 2013-12-17 2022-05-18 Siemens Healthcare Diagnostics Inc Preparación de conjugados de G6PDH mutante de múltiples haptenos y su uso para la detección de múltiples analitos
KR102492243B1 (ko) 2015-02-20 2023-01-25 아이덱스 래보러토리즈, 인코포레이티드 배경 신호에 대한 보상에 의한 균질 면역검정
KR20200073259A (ko) * 2017-10-19 2020-06-23 아이덱스 래보러토리즈, 인코포레이티드 대칭 디메틸아르기닌의 검출

Also Published As

Publication number Publication date
KR20230018536A (ko) 2023-02-07
US11913942B2 (en) 2024-02-27
JP7459018B2 (ja) 2024-04-01
CN107250795B (zh) 2021-07-30
KR102492243B1 (ko) 2023-01-25
MX2017010505A (es) 2017-11-13
EP3259593A1 (en) 2017-12-27
TWI721966B (zh) 2021-03-21
US10775365B2 (en) 2020-09-15
JP2021156896A (ja) 2021-10-07
US20160245801A1 (en) 2016-08-25
EP4170346A1 (en) 2023-04-26
CN113699125A (zh) 2021-11-26
JP6896637B2 (ja) 2021-06-30
JP2018507408A (ja) 2018-03-15
ES2936294T3 (es) 2023-03-15
EP3259593B1 (en) 2022-10-05
HUE060884T2 (hu) 2023-04-28
CN107250795A (zh) 2017-10-13
BR112017017719A2 (pt) 2018-04-10
PL3259593T3 (pl) 2023-02-20
CA2977062A1 (en) 2016-08-25
AU2022204015A1 (en) 2022-06-30
AU2016219847B2 (en) 2022-03-10
FI3259593T3 (fi) 2023-01-13
TW201643428A (zh) 2016-12-16
EP3259593A4 (en) 2018-08-08
AU2016219847A1 (en) 2017-08-24
WO2016134251A1 (en) 2016-08-25
KR20170117574A (ko) 2017-10-23
US20210018496A1 (en) 2021-01-21

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