BR112014020513A2 - Métodos e sistemas para amplificação de sinal de bioensaios - Google Patents

Métodos e sistemas para amplificação de sinal de bioensaios

Info

Publication number
BR112014020513A2
BR112014020513A2 BR112014020513A BR112014020513A BR112014020513A2 BR 112014020513 A2 BR112014020513 A2 BR 112014020513A2 BR 112014020513 A BR112014020513 A BR 112014020513A BR 112014020513 A BR112014020513 A BR 112014020513A BR 112014020513 A2 BR112014020513 A2 BR 112014020513A2
Authority
BR
Brazil
Prior art keywords
systems
methods
analyte
interest
bioassays
Prior art date
Application number
BR112014020513A
Other languages
English (en)
Other versions
BR112014020513B1 (pt
Inventor
J Conrad Andrew
Barrett Hopkins Ben
Lyman Anderson Dwight
Eric Erickson Stephen
Original Assignee
Laboratory Corp America Holdings
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Laboratory Corp America Holdings filed Critical Laboratory Corp America Holdings
Publication of BR112014020513A2 publication Critical patent/BR112014020513A2/pt
Publication of BR112014020513B1 publication Critical patent/BR112014020513B1/pt

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

métodos e sistemas para a amplificação de sinal de bioensaios. a presente invenção refere-se a métodos e sistemas para a detecção de um analito de interesse por um ensaio amplificado. os métodos e sistemas da invenção utilizam a alta especificidade das biomoléculas que foram acopladas a um suporte sólido para amplificar ainda mais um sinal produzido pela interação de um agente ligante com o analito de interesse como um meio para detectar baixos níveis do analito de interesse presente em uma amostra. em determinadas modalidades, para ensaios imunoquímicos (por exemplo, elisa ou ria) ou de imunofluorescência em que uma proteína a ser identificada está ligada diretamente ou por meio de anticorpos de captura a uma superfície passivada, uma amplificação de ~10.000 vezes pode ser alcançada.
BR112014020513-2A 2012-02-21 2013-02-21 Métodos e sistemas para amplificação de sinal de bioensaios BR112014020513B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261601244P 2012-02-21 2012-02-21
US61/601,244 2012-02-21
PCT/US2013/027060 WO2013126522A1 (en) 2012-02-21 2013-02-21 Methods and systems for signal amplification of bioassays

Publications (2)

Publication Number Publication Date
BR112014020513A2 true BR112014020513A2 (pt) 2022-06-21
BR112014020513B1 BR112014020513B1 (pt) 2023-10-03

Family

ID=48982550

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014020513-2A BR112014020513B1 (pt) 2012-02-21 2013-02-21 Métodos e sistemas para amplificação de sinal de bioensaios

Country Status (11)

Country Link
US (2) US20130217049A1 (pt)
EP (1) EP2817423B1 (pt)
JP (2) JP6333181B2 (pt)
CN (1) CN104245960B (pt)
AU (1) AU2013222444B2 (pt)
BR (1) BR112014020513B1 (pt)
CA (2) CA2865303C (pt)
ES (1) ES2636483T3 (pt)
HK (1) HK1205761A1 (pt)
MX (1) MX353566B (pt)
WO (1) WO2013126522A1 (pt)

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Publication number Priority date Publication date Assignee Title
US10378044B1 (en) * 2013-10-01 2019-08-13 FemtoDx Methods for increasing the molecular specificity of a nanosensor
JPWO2017175523A1 (ja) * 2016-04-06 2019-02-14 コニカミノルタ株式会社 蛍光免疫染色法
CN108700576B (zh) * 2017-01-20 2021-07-02 深圳市新产业生物医学工程股份有限公司 制备抗体对的方法、试剂盒及试剂盒的应用和制备抗体对的系统
CN112218720A (zh) * 2017-06-12 2021-01-12 Essenlix公司 均相测定
AU2019248224A1 (en) * 2018-04-03 2020-11-26 The Royal Institution For The Advancement Of Learning/Mcgill University Colocalization-by-linkage sandwich assays
CN112415184A (zh) * 2019-08-22 2021-02-26 香港科技大学 用于细胞群体和单细胞的单分子分离系统及其方法和用途
US20210208160A1 (en) * 2020-01-08 2021-07-08 The U.S.A., As Represented By The Secretary, Department Of Health And Human Services Peptides representing epitopes from filoviruses
CN113866407A (zh) * 2021-12-01 2021-12-31 上海思路迪医学检验所有限公司 一种外泌体her2蛋白的磁免疫化学发光检测试剂盒
WO2024143431A1 (ja) * 2022-12-27 2024-07-04 株式会社TearExo 粒子を組み合わせたセンサ

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Also Published As

Publication number Publication date
JP6333181B2 (ja) 2018-05-30
MX353566B (es) 2018-01-18
ES2636483T3 (es) 2017-10-05
HK1205761A1 (en) 2015-12-24
EP2817423A1 (en) 2014-12-31
EP2817423B1 (en) 2017-05-31
AU2013222444A1 (en) 2014-09-18
CA3092717A1 (en) 2013-08-29
EP2817423A4 (en) 2015-08-19
CN104245960B (zh) 2018-11-09
MX2014010068A (es) 2015-04-08
US20130217049A1 (en) 2013-08-22
BR112014020513B1 (pt) 2023-10-03
CA2865303A1 (en) 2013-08-29
JP2015508180A (ja) 2015-03-16
WO2013126522A1 (en) 2013-08-29
CA2865303C (en) 2020-10-27
JP2018054624A (ja) 2018-04-05
US20200132685A1 (en) 2020-04-30
CN104245960A (zh) 2014-12-24
AU2013222444B2 (en) 2018-04-19

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B09Y Publication of grant cancelled [chapter 9.1.2 patent gazette]

Free format text: ANULADA A PUBLICACAO CODIGO 9.1 NA RPI NO 2683 DE 07/06/2022 POR TER SIDO INDEVIDA.

B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 21/02/2013, OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF, QUE DETERMINA A ALTERACAO DO PRAZO DE CONCESSAO.