MX2014010068A - Metodos y sistemas para amplificacion de señal de bioensayos. - Google Patents
Metodos y sistemas para amplificacion de señal de bioensayos.Info
- Publication number
- MX2014010068A MX2014010068A MX2014010068A MX2014010068A MX2014010068A MX 2014010068 A MX2014010068 A MX 2014010068A MX 2014010068 A MX2014010068 A MX 2014010068A MX 2014010068 A MX2014010068 A MX 2014010068A MX 2014010068 A MX2014010068 A MX 2014010068A
- Authority
- MX
- Mexico
- Prior art keywords
- systems
- analyte
- methods
- interest
- bioassays
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
Abstract
Se describen en la presente métodos y sistemas para la detección de un analito de interés por un ensayo amplificado. El método y sistemas de la invención utilizan la alta especificidad de biomoléculas que han sido acopladas a un soporte sólido para amplificar adicionalmente una señal producida por la interacción de un agente de unión con el analito de interés como un medio para detectar bajos niveles del analito de interés presente en una muestra. En ciertas modalidades, para ensayos inmunoquímicos (por ejemplo, ELISA o RIA) o de inmunofluorescencia, en los cuales una proteína a ser identificada es unida directamente o a través de anticuerpos de captura a una superficie pasivada, puede alcanzarse una amplificación de ~10,000 veces.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201261601244P | 2012-02-21 | 2012-02-21 | |
PCT/US2013/027060 WO2013126522A1 (en) | 2012-02-21 | 2013-02-21 | Methods and systems for signal amplification of bioassays |
Publications (2)
Publication Number | Publication Date |
---|---|
MX2014010068A true MX2014010068A (es) | 2015-04-08 |
MX353566B MX353566B (es) | 2018-01-18 |
Family
ID=48982550
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2014010068A MX353566B (es) | 2012-02-21 | 2013-02-21 | Metodos y sistemas para amplificacion de señal de bioensayos. |
Country Status (11)
Country | Link |
---|---|
US (2) | US20130217049A1 (es) |
EP (1) | EP2817423B1 (es) |
JP (2) | JP6333181B2 (es) |
CN (1) | CN104245960B (es) |
AU (1) | AU2013222444B2 (es) |
BR (1) | BR112014020513B1 (es) |
CA (2) | CA3092717A1 (es) |
ES (1) | ES2636483T3 (es) |
HK (1) | HK1205761A1 (es) |
MX (1) | MX353566B (es) |
WO (1) | WO2013126522A1 (es) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10378044B1 (en) * | 2013-10-01 | 2019-08-13 | FemtoDx | Methods for increasing the molecular specificity of a nanosensor |
US20190079081A1 (en) * | 2016-04-06 | 2019-03-14 | Konica Minolta, Inc. | Fluorescent immunostaining method |
EP3376226B1 (en) * | 2017-01-20 | 2020-05-13 | Shenzhen New Industries Biomedical Engineering Co., Ltd. | Method and kit for preparing antibody pair and use of kit, and system for preparing antibody pair |
JP7339245B2 (ja) * | 2017-06-12 | 2023-09-05 | エッセンリックス コーポレーション | 均質アッセイ法 |
BR112020020108A2 (pt) * | 2018-04-03 | 2021-01-26 | The Royal Institution For The Advancement Of Learning / Mcgill University | ensaios sanduíche de colocalização por ligação |
US20210055290A1 (en) * | 2019-08-22 | 2021-02-25 | The Hong Kong University Of Science And Technology | System for single-molecule isolation for cell populations and single cells, and methods and uses thereof |
US20210208160A1 (en) * | 2020-01-08 | 2021-07-08 | The U.S.A., As Represented By The Secretary, Department Of Health And Human Services | Peptides representing epitopes from filoviruses |
CN113866407A (zh) * | 2021-12-01 | 2021-12-31 | 上海思路迪医学检验所有限公司 | 一种外泌体her2蛋白的磁免疫化学发光检测试剂盒 |
Family Cites Families (23)
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US4098876A (en) * | 1976-10-26 | 1978-07-04 | Corning Glass Works | Reverse sandwich immunoassay |
US4737453A (en) * | 1984-12-12 | 1988-04-12 | Immunomedics, Inc. | Sandwich immunoassay utilizing a separation specific binding substance |
US5149626A (en) * | 1987-05-14 | 1992-09-22 | Mclean Hospital Corporation | Multiple antigen immunoassay |
US5077198A (en) * | 1988-04-14 | 1991-12-31 | Eastman Kodak Company | Diagnostic kit and method for rapid detection of antibodies |
US5567591A (en) * | 1991-06-24 | 1996-10-22 | Becton Dickinson And Company | Amplified assay for analyte |
EP0643777A4 (en) * | 1992-01-22 | 1995-06-07 | Abbott Lab | CALIBRATION REAGENTS FOR SEMI-QUANTITATIVE BINDING ASSAYS AND DEVICES. |
WO1994007138A1 (en) * | 1992-09-14 | 1994-03-31 | Fodstad Oystein | Detection of specific target cells in specialized or mixed cell population and solutions containing mixed cell populations |
US5876944A (en) * | 1996-06-10 | 1999-03-02 | Bayer Corporation | Method for amplification of the response signal in a sandwich immunoassay |
US20030054376A1 (en) * | 1997-07-07 | 2003-03-20 | Mullis Kary Banks | Dual bead assays using cleavable spacers and/or ligation to improve specificity and sensitivity including related methods and apparatus |
JP2000028613A (ja) * | 1998-07-07 | 2000-01-28 | Nitto Denko Corp | 免疫学的検査方法および免疫学的検査キット |
US6696304B1 (en) * | 1999-02-24 | 2004-02-24 | Luminex Corporation | Particulate solid phase immobilized protein quantitation |
WO2002076248A2 (en) * | 2001-03-22 | 2002-10-03 | Minerva Biotechnologies Corporation | Customized therapeutics and in situ diagnostics |
KR20040083411A (ko) * | 2001-08-23 | 2004-10-01 | 큐티엘 바이오시스템즈 엘엘씨 | 생체 분자의 검출 및 정량을 위한 바이오센싱 플랫폼 |
AU2003251428A1 (en) * | 2002-06-10 | 2003-12-22 | The Government Of The United States, As Represented By The Secretary Of The Department Of Health And | Novel molecular conjugates for signal amplification |
US20040018495A1 (en) | 2002-07-24 | 2004-01-29 | Xing-Xiang Li | Microparticle based signal amplification for the detection of analytes |
US20040137430A1 (en) * | 2002-10-15 | 2004-07-15 | Regents Of The University Of Minnesota | Assays to detect or quantify bacterial or viral pathogens and contaminants |
CN1905806A (zh) * | 2003-10-28 | 2007-01-31 | 佰尔瑞溶液有限公司 | 带有吸附的或结合的生物分子的胶-壳珠子 |
WO2005089524A2 (en) * | 2004-03-19 | 2005-09-29 | U.S. Genomics, Inc. | Compositions and methods for detection of single molecules |
ES2523316T3 (es) * | 2004-11-01 | 2014-11-24 | Erber Aktiengesellschaft | Bacteriófagos como agentes selectivos |
EP2092084B1 (en) * | 2006-11-13 | 2016-02-24 | PerkinElmer LAS, Inc. | Detecting molecular interactions |
CN102227631B (zh) * | 2008-11-28 | 2014-04-23 | 英佛皮亚有限公司 | 免疫层析测试中信号放大的方法以及使用该方法的免疫层析试剂盒 |
WO2010089138A1 (en) * | 2009-02-09 | 2010-08-12 | Caprotec Bioanalytics Gmbh | Devices, systems and methods for separating magnetic particles |
EP2594942A1 (en) * | 2011-11-16 | 2013-05-22 | Koninklijke Philips Electronics N.V. | Long rigid spacers to enhance binding kinetics in immunoassays |
-
2013
- 2013-02-21 CA CA3092717A patent/CA3092717A1/en active Pending
- 2013-02-21 CA CA2865303A patent/CA2865303C/en active Active
- 2013-02-21 AU AU2013222444A patent/AU2013222444B2/en not_active Ceased
- 2013-02-21 ES ES13752350.2T patent/ES2636483T3/es active Active
- 2013-02-21 BR BR112014020513-2A patent/BR112014020513B1/pt active IP Right Grant
- 2013-02-21 WO PCT/US2013/027060 patent/WO2013126522A1/en active Application Filing
- 2013-02-21 US US13/772,887 patent/US20130217049A1/en not_active Abandoned
- 2013-02-21 EP EP13752350.2A patent/EP2817423B1/en not_active Not-in-force
- 2013-02-21 MX MX2014010068A patent/MX353566B/es active IP Right Grant
- 2013-02-21 JP JP2014558816A patent/JP6333181B2/ja not_active Expired - Fee Related
- 2013-02-21 CN CN201380017257.1A patent/CN104245960B/zh not_active Expired - Fee Related
-
2015
- 2015-06-29 HK HK15106180.0A patent/HK1205761A1/xx unknown
-
2017
- 2017-11-21 JP JP2017223733A patent/JP2018054624A/ja active Pending
-
2019
- 2019-08-21 US US16/546,647 patent/US20200132685A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP2817423A4 (en) | 2015-08-19 |
WO2013126522A1 (en) | 2013-08-29 |
BR112014020513B1 (pt) | 2023-10-03 |
AU2013222444A1 (en) | 2014-09-18 |
MX353566B (es) | 2018-01-18 |
JP2018054624A (ja) | 2018-04-05 |
ES2636483T3 (es) | 2017-10-05 |
US20130217049A1 (en) | 2013-08-22 |
CN104245960B (zh) | 2018-11-09 |
EP2817423A1 (en) | 2014-12-31 |
EP2817423B1 (en) | 2017-05-31 |
JP2015508180A (ja) | 2015-03-16 |
AU2013222444B2 (en) | 2018-04-19 |
JP6333181B2 (ja) | 2018-05-30 |
US20200132685A1 (en) | 2020-04-30 |
CA2865303A1 (en) | 2013-08-29 |
BR112014020513A2 (pt) | 2022-06-21 |
CN104245960A (zh) | 2014-12-24 |
CA2865303C (en) | 2020-10-27 |
CA3092717A1 (en) | 2013-08-29 |
HK1205761A1 (en) | 2015-12-24 |
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