TR201907065T4 - Aptamer kullanarak hedef maddeyi tespit yöntemi. - Google Patents
Aptamer kullanarak hedef maddeyi tespit yöntemi. Download PDFInfo
- Publication number
- TR201907065T4 TR201907065T4 TR2019/07065T TR201907065T TR201907065T4 TR 201907065 T4 TR201907065 T4 TR 201907065T4 TR 2019/07065 T TR2019/07065 T TR 2019/07065T TR 201907065 T TR201907065 T TR 201907065T TR 201907065 T4 TR201907065 T4 TR 201907065T4
- Authority
- TR
- Turkey
- Prior art keywords
- aptamer
- detecting
- target substance
- target material
- kit
- Prior art date
Links
- 108091023037 Aptamer Proteins 0.000 title abstract 6
- 239000013076 target substance Substances 0.000 title 1
- 239000013077 target material Substances 0.000 abstract 3
- 239000007790 solid phase Substances 0.000 abstract 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G01N33/5438—Electrodes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/16—Aptamers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/10—Applications; Uses in screening processes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Mevcut buluş, bir aptamer kullanarak bir hedef materyali tespit etmek için bir metot ve kite ve daha özel olarak, birinci aptamer, hedef malzeme ve ikinci aptamer arasında sandviçlenmiş bir bağ oluşturmak üzere katı bir fazda immobilize edilmiş bir birinci aptamere, bir numune ve ikinci aptamerin eklendiği bir hedef materyali tespit etmek için bir metot ve kit ile ilgilidir.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020090012288A KR101437616B1 (ko) | 2009-02-16 | 2009-02-16 | 압타머를 이용한 fet 센서 기반 타겟물질의 검출방법 |
KR20090012287 | 2009-02-16 |
Publications (1)
Publication Number | Publication Date |
---|---|
TR201907065T4 true TR201907065T4 (tr) | 2019-06-21 |
Family
ID=42562220
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
TR2019/07065T TR201907065T4 (tr) | 2009-02-16 | 2010-02-16 | Aptamer kullanarak hedef maddeyi tespit yöntemi. |
Country Status (6)
Country | Link |
---|---|
US (1) | US9075053B2 (tr) |
EP (3) | EP3495501B1 (tr) |
CN (1) | CN102395684B (tr) |
ES (1) | ES2730808T3 (tr) |
TR (1) | TR201907065T4 (tr) |
WO (1) | WO2010093223A2 (tr) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101892540B1 (ko) * | 2012-05-10 | 2018-08-28 | 삼성전자주식회사 | 바이오 물질의 알에프 특성 측정 방법 및 장치 |
US9310357B2 (en) | 2012-09-28 | 2016-04-12 | Src, Inc. | Detection of chemical and biological agents using oligonucleotide aptamers |
CN102980888A (zh) * | 2012-12-18 | 2013-03-20 | 合肥工业大学 | 基于核酸适配体探针的一步法非标记型双酚a的快速比色检测法 |
DE102013211125A1 (de) * | 2013-06-14 | 2014-12-18 | Siemens Aktiengesellschaft | Verfahren zur kombinierten Quantifizierung und Sequenzierung von mindestens einer Ziel-Nukleinsäure |
WO2015066027A2 (en) | 2013-10-28 | 2015-05-07 | Dots Devices, Inc. | Allergen detection |
JP2018519796A (ja) | 2015-04-29 | 2018-07-26 | ドッツ テクノロジー コーポレイションDOTS Technology Corp. | アレルゲン検出のための組成物及び方法 |
JP7482795B2 (ja) * | 2018-12-21 | 2024-05-14 | イルミナ インコーポレイテッド | 感知システム |
WO2021062475A1 (en) * | 2019-10-01 | 2021-04-08 | WearOptimo Pty Ltd | Analyte measurement system |
US11488326B2 (en) | 2019-12-11 | 2022-11-01 | Ananya Achanta | Estimation of bisphenol a using image analysis |
CN112098487A (zh) * | 2020-08-08 | 2020-12-18 | 青岛科技大学 | 一种纳米孔道光电化学dna传感器及其制备方法与应用 |
CN113960128B (zh) * | 2020-12-15 | 2022-08-23 | 有研工程技术研究院有限公司 | 基于钾离子适配体修饰的硅纳米线场效应管生物传感器 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001079562A1 (en) * | 2000-04-18 | 2001-10-25 | Gilead Sciences, Inc. | Aptamer based two-site binding assay |
WO2002053778A2 (en) * | 2001-01-05 | 2002-07-11 | Genomicfx, Inc. | Method for relative quantification of attached nucleic acids |
US20030064530A1 (en) * | 2001-07-04 | 2003-04-03 | Nitto Denko Corporation | Aptamer capable of specifically adsorbing to bisphenol A and method for obtaining the aptamer |
EP1660858A4 (en) * | 2003-07-21 | 2007-10-24 | Amplified Proteomics Inc | MULTIPLEX analyte |
KR100828481B1 (ko) * | 2006-12-22 | 2008-05-13 | 고려대학교 산학협력단 | 환경호르몬에 특이적으로 결합하는 dna 앱타머를 이용한환경호르몬 제거방법 |
US20110065086A1 (en) * | 2008-02-21 | 2011-03-17 | Otc Biotechnologies, Llc | Methods of producing homogeneous plastic-adherent aptamer-magnetic bead-fluorophore and other sandwich assays |
-
2010
- 2010-02-16 EP EP18208862.5A patent/EP3495501B1/en active Active
- 2010-02-16 CN CN201080016373.8A patent/CN102395684B/zh active Active
- 2010-02-16 TR TR2019/07065T patent/TR201907065T4/tr unknown
- 2010-02-16 ES ES17157804T patent/ES2730808T3/es active Active
- 2010-02-16 EP EP10741440.1A patent/EP2397562B1/en active Active
- 2010-02-16 US US13/201,764 patent/US9075053B2/en active Active
- 2010-02-16 WO PCT/KR2010/000952 patent/WO2010093223A2/ko active Application Filing
- 2010-02-16 EP EP17157804.0A patent/EP3192881B1/en active Active
Also Published As
Publication number | Publication date |
---|---|
EP2397562A4 (en) | 2012-08-15 |
EP3495501A1 (en) | 2019-06-12 |
EP3192881A1 (en) | 2017-07-19 |
WO2010093223A2 (ko) | 2010-08-19 |
US9075053B2 (en) | 2015-07-07 |
ES2730808T3 (es) | 2019-11-12 |
EP3495501B1 (en) | 2022-10-05 |
EP2397562A2 (en) | 2011-12-21 |
CN102395684A (zh) | 2012-03-28 |
EP2397562B1 (en) | 2017-08-23 |
US20120040865A1 (en) | 2012-02-16 |
EP3192881B1 (en) | 2019-04-17 |
WO2010093223A3 (ko) | 2011-02-24 |
CN102395684B (zh) | 2015-06-03 |
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