JP5984267B2 - 活性型の可溶性シアリルトランスフェラーゼを作製及び精製する方法 - Google Patents

活性型の可溶性シアリルトランスフェラーゼを作製及び精製する方法 Download PDF

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JP5984267B2
JP5984267B2 JP2013522234A JP2013522234A JP5984267B2 JP 5984267 B2 JP5984267 B2 JP 5984267B2 JP 2013522234 A JP2013522234 A JP 2013522234A JP 2013522234 A JP2013522234 A JP 2013522234A JP 5984267 B2 JP5984267 B2 JP 5984267B2
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sialyltransferase
chromatography
polypeptide
st6galnaci
exchange chromatography
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JP2013535207A5 (enExample
JP2013535207A (ja
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アクセル アンガーマン
アクセル アンガーマン
クリスティアン シェッカーマン
クリスティアン シェッカーマン
カルステン シュミット
カルステン シュミット
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ラティオファーム ゲーエムベーハー
ラティオファーム ゲーエムベーハー
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C12Y204/99003—Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase (2.4.99.3)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicinal Preparation (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2013522234A 2010-08-02 2011-08-02 活性型の可溶性シアリルトランスフェラーゼを作製及び精製する方法 Active JP5984267B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20100171626 EP2415779B1 (en) 2010-08-02 2010-08-02 Method of producing and purifying an active soluble sialyltransferase
EP10171626.4 2010-08-02
PCT/EP2011/063291 WO2012016984A1 (en) 2010-08-02 2011-08-02 Method of producing and purifying an active soluble sialyltransferase

Publications (3)

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JP2013535207A JP2013535207A (ja) 2013-09-12
JP2013535207A5 JP2013535207A5 (enExample) 2014-09-11
JP5984267B2 true JP5984267B2 (ja) 2016-09-06

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JP2013522234A Active JP5984267B2 (ja) 2010-08-02 2011-08-02 活性型の可溶性シアリルトランスフェラーゼを作製及び精製する方法

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US (1) US9222081B2 (enExample)
EP (2) EP2415779B1 (enExample)
JP (1) JP5984267B2 (enExample)
CN (1) CN103154017B (enExample)
CA (1) CA2807397C (enExample)
ES (1) ES2534936T3 (enExample)
MX (1) MX2013001447A (enExample)
PT (1) PT2415779E (enExample)
WO (1) WO2012016984A1 (enExample)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013075740A1 (en) * 2011-11-23 2013-05-30 Sanofi Antibody purification method
CN105026416A (zh) * 2013-02-06 2015-11-04 新加坡科技研究局 用于降低蛋白质制剂中的聚集物含量的混合的多官能性金属亲和表面
ES2665645T3 (es) 2013-05-06 2018-04-26 Sanofi Proceso multietapa continuo para purificar anticuerpos
KR20170132293A (ko) * 2015-03-31 2017-12-01 예다 리서치 앤드 디벨럽먼트 캄파니 리미티드 글루타메이트 옥살로아세테이트 트랜스아미나제 1（ｇｏｔ１） 제제와 이의 제조방법 및 용도
KR101667677B1 (ko) * 2015-05-29 2016-10-19 한국생명공학연구원 재조합 ST6Gal1 단백질을 활성형으로 제조하는 방법
CN110100007B (zh) * 2016-12-21 2024-05-28 豪夫迈·罗氏有限公司 用于体外糖工程化抗体的酶的再使用
WO2018114879A1 (en) 2016-12-21 2018-06-28 F. Hoffmann-La Roche Ag Method for in vitro glycoengineering of antibodies
CA3074380C (en) 2017-09-04 2023-01-03 89Bio Ltd. Mutant fgf-21 peptide conjugates and uses thereof
WO2019063849A1 (en) * 2017-12-20 2019-04-04 Dsm Ip Assets B.V. PURIFICATION OF A POLYPEPTIDE OF INTEREST
EP3546475A1 (en) 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
KR102362777B1 (ko) * 2018-03-27 2022-02-15 주식회사 녹십자 친화성 크로마토그래피 공정을 포함하는 백일해균 유래 단백질 수득 방법
KR102140531B1 (ko) * 2018-08-07 2020-08-04 (주)휴온스 Gly-Tβ4의 제조방법
EP3613486B1 (de) * 2018-08-24 2020-10-07 UGA Biopharma GmbH Verfahren und anlage zur reinigung von epo und/oder einem epo-derivat
CN113416243B (zh) * 2021-08-23 2021-12-10 上海盛迪医药有限公司 一种纯化抗体的方法
CN119912505A (zh) * 2025-04-02 2025-05-02 浙江熙正霖生物科技有限公司 一种从发酵液中提纯唾液酸的方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5032519A (en) * 1989-10-24 1991-07-16 The Regents Of The Univ. Of California Method for producing secretable glycosyltransferases and other Golgi processing enzymes
WO1995018217A1 (en) 1993-12-24 1995-07-06 The Institute Of Physical And Chemical Research Novel sugar-chain synthetase and process for producing the same
US6280989B1 (en) * 1999-06-17 2001-08-28 Dmitri Kapitonov Sialyltransferases
EP2305312B1 (en) 2001-10-10 2015-03-04 ratiopharm GmbH Remodelling and glycoconjugation of follicle-stimulating hormone (FSH)
EP1694274B1 (en) * 2003-12-03 2013-04-24 BioGeneriX AG Glycopegylated granulocyte colony stimulating factor
WO2005121332A2 (en) 2004-06-03 2005-12-22 Neose Technologies, Inc. Truncated st6galnaci polypeptides and nucleic acids
US8137928B2 (en) 2005-03-24 2012-03-20 BioGeneriX Expression of soluble, active eukaryotic glycosyltransferases in prokaryotic organisms
EP1866427A4 (en) * 2005-03-30 2010-09-01 Novo Nordisk As METHOD OF MANUFACTURING PEPTIDES PRODUCED IN INSECT CELL LINES
US20090047710A1 (en) 2005-11-09 2009-02-19 Neose Technologies, Inc. ST3Gal-1/ST6GalNAc-1 Chimeras
US20100075375A1 (en) * 2006-10-03 2010-03-25 Novo Nordisk A/S Methods for the purification of polypeptide conjugates
WO2008073620A2 (en) 2006-11-02 2008-06-19 Neose Technologies, Inc. Manufacturing process for the production of polypeptides expressed in insect cell-lines

Also Published As

Publication number Publication date
ES2534936T3 (es) 2015-04-30
EP2601207B1 (en) 2016-12-28
WO2012016984A1 (en) 2012-02-09
CN103154017B (zh) 2016-02-03
CA2807397C (en) 2019-03-19
CA2807397A1 (en) 2012-02-09
US9222081B2 (en) 2015-12-29
EP2415779A1 (en) 2012-02-08
EP2415779B1 (en) 2015-01-21
CN103154017A (zh) 2013-06-12
PT2415779E (pt) 2015-05-13
MX2013001447A (es) 2013-11-20
US20140057316A1 (en) 2014-02-27
JP2013535207A (ja) 2013-09-12
EP2601207A1 (en) 2013-06-12

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