CN103154017B - 生产和纯化活性可溶唾液酸转移酶的方法 - Google Patents

生产和纯化活性可溶唾液酸转移酶的方法 Download PDF

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Publication number
CN103154017B
CN103154017B CN201180047627.7A CN201180047627A CN103154017B CN 103154017 B CN103154017 B CN 103154017B CN 201180047627 A CN201180047627 A CN 201180047627A CN 103154017 B CN103154017 B CN 103154017B
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CN
China
Prior art keywords
chromatography
sialyltransferase
st6galnaci
exchange chromatography
resin
Prior art date
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Expired - Fee Related
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CN201180047627.7A
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English (en)
Chinese (zh)
Other versions
CN103154017A (zh
Inventor
A·安格曼
C·谢克曼
K·施密特
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Ratiopharm GmbH
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Ratiopharm GmbH
Biogenerix GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C12Y204/99003—Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase (2.4.99.3)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicinal Preparation (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201180047627.7A 2010-08-02 2011-08-02 生产和纯化活性可溶唾液酸转移酶的方法 Expired - Fee Related CN103154017B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP10171626.4 2010-08-02
EP20100171626 EP2415779B1 (en) 2010-08-02 2010-08-02 Method of producing and purifying an active soluble sialyltransferase
PCT/EP2011/063291 WO2012016984A1 (en) 2010-08-02 2011-08-02 Method of producing and purifying an active soluble sialyltransferase

Publications (2)

Publication Number Publication Date
CN103154017A CN103154017A (zh) 2013-06-12
CN103154017B true CN103154017B (zh) 2016-02-03

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Family Applications (1)

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CN201180047627.7A Expired - Fee Related CN103154017B (zh) 2010-08-02 2011-08-02 生产和纯化活性可溶唾液酸转移酶的方法

Country Status (9)

Country Link
US (1) US9222081B2 (enExample)
EP (2) EP2415779B1 (enExample)
JP (1) JP5984267B2 (enExample)
CN (1) CN103154017B (enExample)
CA (1) CA2807397C (enExample)
ES (1) ES2534936T3 (enExample)
MX (1) MX2013001447A (enExample)
PT (1) PT2415779E (enExample)
WO (1) WO2012016984A1 (enExample)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013075740A1 (en) * 2011-11-23 2013-05-30 Sanofi Antibody purification method
JP2016513245A (ja) * 2013-02-06 2016-05-12 エイジェンシー・フォー・サイエンス，テクノロジー・アンド・リサーチ タンパク質製剤の凝集体含有量を減少させる混合多官能金属親和性表面
DK2994485T3 (en) 2013-05-06 2018-04-23 Sanofi Sa CONTINUOUS MULTIPLE STEP PROCEDURE FOR PURIFICATION OF ANTIBODIES
KR20170132293A (ko) * 2015-03-31 2017-12-01 예다 리서치 앤드 디벨럽먼트 캄파니 리미티드 글루타메이트 옥살로아세테이트 트랜스아미나제 1（ｇｏｔ１） 제제와 이의 제조방법 및 용도
KR101667677B1 (ko) * 2015-05-29 2016-10-19 한국생명공학연구원 재조합 ST6Gal1 단백질을 활성형으로 제조하는 방법
MX2019006123A (es) 2016-12-21 2019-08-12 Hoffmann La Roche Metodo para glicomanipulacion in vitro de anticuerpos.
MX2019007411A (es) * 2016-12-21 2019-08-29 Hoffmann La Roche Reuso de enzimas en glucomanipulacion in vitro de anticuerpos.
WO2019043457A2 (en) 2017-09-04 2019-03-07 89Bio Ltd. CONJUGATES OF MUTANT PEPTIDE FGF-21 AND USES THEREOF
WO2019063849A1 (en) * 2017-12-20 2019-04-04 Dsm Ip Assets B.V. PURIFICATION OF A POLYPEPTIDE OF INTEREST
EP3546475A1 (en) 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
KR102362777B1 (ko) * 2018-03-27 2022-02-15 주식회사 녹십자 친화성 크로마토그래피 공정을 포함하는 백일해균 유래 단백질 수득 방법
KR102140531B1 (ko) * 2018-08-07 2020-08-04 (주)휴온스 Gly-Tβ4의 제조방법
DK3613486T3 (da) * 2018-08-24 2021-01-04 Uga Biopharma Gmbh Metode og anlæg til rengøring af epo og/eller et epo-derivat
CN113416243B (zh) * 2021-08-23 2021-12-10 上海盛迪医药有限公司 一种纯化抗体的方法
CN119912505A (zh) * 2025-04-02 2025-05-02 浙江熙正霖生物科技有限公司 一种从发酵液中提纯唾液酸的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6280989B1 (en) * 1999-06-17 2001-08-28 Dmitri Kapitonov Sialyltransferases
WO2005121332A2 (en) * 2004-06-03 2005-12-22 Neose Technologies, Inc. Truncated st6galnaci polypeptides and nucleic acids

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5032519A (en) 1989-10-24 1991-07-16 The Regents Of The Univ. Of California Method for producing secretable glycosyltransferases and other Golgi processing enzymes
WO1995018217A1 (en) 1993-12-24 1995-07-06 The Institute Of Physical And Chemical Research Novel sugar-chain synthetase and process for producing the same
EP2292273A3 (en) 2001-10-10 2011-11-23 BioGeneriX AG Remodeling and glycoconjugation of peptides
ES2422187T3 (es) * 2003-12-03 2013-09-09 Biogenerix Ag Factor estimulante de colonias de granulocitos glicopegilados
JP5235657B2 (ja) 2005-03-24 2013-07-10 バイオジェネリックス ゲーエムベーハー 原核生物における可溶性活性真核生物グリコシルトランスフェラーゼの発現
US20060246544A1 (en) 2005-03-30 2006-11-02 Neose Technologies,Inc. Manufacturing process for the production of peptides grown in insect cell lines
US20090047710A1 (en) 2005-11-09 2009-02-19 Neose Technologies, Inc. ST3Gal-1/ST6GalNAc-1 Chimeras
US8969532B2 (en) * 2006-10-03 2015-03-03 Novo Nordisk A/S Methods for the purification of polypeptide conjugates comprising polyalkylene oxide using hydrophobic interaction chromatography
WO2008073620A2 (en) 2006-11-02 2008-06-19 Neose Technologies, Inc. Manufacturing process for the production of polypeptides expressed in insect cell-lines

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6280989B1 (en) * 1999-06-17 2001-08-28 Dmitri Kapitonov Sialyltransferases
WO2005121332A2 (en) * 2004-06-03 2005-12-22 Neose Technologies, Inc. Truncated st6galnaci polypeptides and nucleic acids

Also Published As

Publication number Publication date
EP2415779A1 (en) 2012-02-08
ES2534936T3 (es) 2015-04-30
JP2013535207A (ja) 2013-09-12
MX2013001447A (es) 2013-11-20
EP2601207A1 (en) 2013-06-12
EP2415779B1 (en) 2015-01-21
WO2012016984A1 (en) 2012-02-09
US20140057316A1 (en) 2014-02-27
EP2601207B1 (en) 2016-12-28
PT2415779E (pt) 2015-05-13
CA2807397A1 (en) 2012-02-09
JP5984267B2 (ja) 2016-09-06
CA2807397C (en) 2019-03-19
US9222081B2 (en) 2015-12-29
CN103154017A (zh) 2013-06-12

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