CA2807397C - Method of producing and purifying an active soluble sialyltransferase - Google Patents
Method of producing and purifying an active soluble sialyltransferase Download PDFInfo
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- CA2807397C CA2807397C CA2807397A CA2807397A CA2807397C CA 2807397 C CA2807397 C CA 2807397C CA 2807397 A CA2807397 A CA 2807397A CA 2807397 A CA2807397 A CA 2807397A CA 2807397 C CA2807397 C CA 2807397C
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- sialyltransferase
- polypeptide
- chromatography
- resin
- st6galnaci
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
- C12Y204/99003—Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase (2.4.99.3)
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicinal Preparation (AREA)
- Peptides Or Proteins (AREA)
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| EP20100171626 EP2415779B1 (en) | 2010-08-02 | 2010-08-02 | Method of producing and purifying an active soluble sialyltransferase |
| EP10171626.4 | 2010-08-02 | ||
| PCT/EP2011/063291 WO2012016984A1 (en) | 2010-08-02 | 2011-08-02 | Method of producing and purifying an active soluble sialyltransferase |
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| CA2807397A1 CA2807397A1 (en) | 2012-02-09 |
| CA2807397C true CA2807397C (en) | 2019-03-19 |
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| EP (2) | EP2415779B1 (enExample) |
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| MX (1) | MX2013001447A (enExample) |
| PT (1) | PT2415779E (enExample) |
| WO (1) | WO2012016984A1 (enExample) |
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| WO2013075740A1 (en) | 2011-11-23 | 2013-05-30 | Sanofi | Antibody purification method |
| EP2953963A4 (en) * | 2013-02-06 | 2016-11-09 | Agency Science Tech & Res | SURFACES WITH MIXED MULTIFUNCTIONAL METAL-AFFINITY TO REDUCE AGGREGATE CONTENT IN PROTEIN PREPARATIONS |
| AU2014264697B2 (en) | 2013-05-06 | 2019-08-22 | Sanofi | Continuous multistep process for purifying antibodies |
| KR20170132293A (ko) * | 2015-03-31 | 2017-12-01 | 예다 리서치 앤드 디벨럽먼트 캄파니 리미티드 | 글루타메이트 옥살로아세테이트 트랜스아미나제 1（ｇｏｔ１） 제제와 이의 제조방법 및 용도 |
| KR101667677B1 (ko) * | 2015-05-29 | 2016-10-19 | 한국생명공학연구원 | 재조합 ST6Gal1 단백질을 활성형으로 제조하는 방법 |
| CN110088292A (zh) | 2016-12-21 | 2019-08-02 | 豪夫迈·罗氏有限公司 | 用于体外糖工程化抗体的方法 |
| KR102390246B1 (ko) * | 2016-12-21 | 2022-04-22 | 에프. 호프만-라 로슈 아게 | 항체의 시험관내 글리코조작에 있어서의 효소의 재사용 |
| ES2930534T3 (es) | 2017-09-04 | 2022-12-16 | 89Bio Ltd | Conjugados de péptidos de FGF-21 mutantes y usos de los mismos |
| WO2019063849A1 (en) * | 2017-12-20 | 2019-04-04 | Dsm Ip Assets B.V. | PURIFICATION OF A POLYPEPTIDE OF INTEREST |
| KR102362777B1 (ko) * | 2018-03-27 | 2022-02-15 | 주식회사 녹십자 | 친화성 크로마토그래피 공정을 포함하는 백일해균 유래 단백질 수득 방법 |
| EP3546475A1 (en) | 2018-03-27 | 2019-10-02 | Sanofi | Full flow-through process for purifying recombinant proteins |
| KR102140531B1 (ko) * | 2018-08-07 | 2020-08-04 | (주)휴온스 | Gly-Tβ4의 제조방법 |
| DK3613486T3 (da) * | 2018-08-24 | 2021-01-04 | Uga Biopharma Gmbh | Metode og anlæg til rengøring af epo og/eller et epo-derivat |
| CN113416243B (zh) * | 2021-08-23 | 2021-12-10 | 上海盛迪医药有限公司 | 一种纯化抗体的方法 |
| CN119912505A (zh) * | 2025-04-02 | 2025-05-02 | 浙江熙正霖生物科技有限公司 | 一种从发酵液中提纯唾液酸的方法 |
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| US5032519A (en) * | 1989-10-24 | 1991-07-16 | The Regents Of The Univ. Of California | Method for producing secretable glycosyltransferases and other Golgi processing enzymes |
| WO1995018217A1 (en) | 1993-12-24 | 1995-07-06 | The Institute Of Physical And Chemical Research | Novel sugar-chain synthetase and process for producing the same |
| US6280989B1 (en) * | 1999-06-17 | 2001-08-28 | Dmitri Kapitonov | Sialyltransferases |
| ES2411007T3 (es) | 2001-10-10 | 2013-07-04 | Novo Nordisk A/S | Remodelación y glicoconjugación de péptidos |
| BRPI0417342A (pt) * | 2003-12-03 | 2007-04-17 | Neose Technologies Inc | fator estimulante de colÈnia de granulócitos glicopeguilado |
| EP1765993A4 (en) | 2004-06-03 | 2008-08-20 | Neose Technologies Inc | ST6GALNACL TRUNC POLYPEPTIDES AND NUCLEIC ACIDS |
| KR20080021590A (ko) | 2005-03-24 | 2008-03-07 | 네오스 테크놀로지스, 인크. | 가용성이고 활성인 진핵생물 글리코실트랜스퍼라제의원핵생물 유기체 내에서의 발현 |
| JP2008538181A (ja) * | 2005-03-30 | 2008-10-16 | ネオス テクノロジーズ インコーポレイテッド | 昆虫細胞系において増殖させたペプチドを生産するための製造方法 |
| US20090047710A1 (en) | 2005-11-09 | 2009-02-19 | Neose Technologies, Inc. | ST3Gal-1/ST6GalNAc-1 Chimeras |
| WO2008057683A2 (en) * | 2006-10-03 | 2008-05-15 | Novo Nordisk A/S | Methods for the purification of polypeptide conjugates |
| WO2008073620A2 (en) | 2006-11-02 | 2008-06-19 | Neose Technologies, Inc. | Manufacturing process for the production of polypeptides expressed in insect cell-lines |
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- 2011-08-02 US US13/813,804 patent/US9222081B2/en active Active
- 2011-08-02 CN CN201180047627.7A patent/CN103154017B/zh not_active Expired - Fee Related
- 2011-08-02 EP EP11738247.3A patent/EP2601207B1/en active Active
- 2011-08-02 MX MX2013001447A patent/MX2013001447A/es unknown
- 2011-08-02 WO PCT/EP2011/063291 patent/WO2012016984A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| US9222081B2 (en) | 2015-12-29 |
| US20140057316A1 (en) | 2014-02-27 |
| EP2601207B1 (en) | 2016-12-28 |
| EP2601207A1 (en) | 2013-06-12 |
| CN103154017B (zh) | 2016-02-03 |
| PT2415779E (pt) | 2015-05-13 |
| MX2013001447A (es) | 2013-11-20 |
| CN103154017A (zh) | 2013-06-12 |
| JP2013535207A (ja) | 2013-09-12 |
| CA2807397A1 (en) | 2012-02-09 |
| EP2415779A1 (en) | 2012-02-08 |
| WO2012016984A1 (en) | 2012-02-09 |
| JP5984267B2 (ja) | 2016-09-06 |
| ES2534936T3 (es) | 2015-04-30 |
| EP2415779B1 (en) | 2015-01-21 |
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