JP2013535207A5 - - Google Patents

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Publication number
JP2013535207A5
JP2013535207A5 JP2013522234A JP2013522234A JP2013535207A5 JP 2013535207 A5 JP2013535207 A5 JP 2013535207A5 JP 2013522234 A JP2013522234 A JP 2013522234A JP 2013522234 A JP2013522234 A JP 2013522234A JP 2013535207 A5 JP2013535207 A5 JP 2013535207A5
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JP
Japan
Prior art keywords
chromatography
exchange chromatography
sialyltransferase
resin
cation exchange
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Application number
JP2013522234A
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English (en)
Japanese (ja)
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JP5984267B2 (ja
JP2013535207A (ja
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Publication date
Priority claimed from EP20100171626 external-priority patent/EP2415779B1/en
Application filed filed Critical
Publication of JP2013535207A publication Critical patent/JP2013535207A/ja
Publication of JP2013535207A5 publication Critical patent/JP2013535207A5/ja
Application granted granted Critical
Publication of JP5984267B2 publication Critical patent/JP5984267B2/ja
Active legal-status Critical Current
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JP2013522234A 2010-08-02 2011-08-02 活性型の可溶性シアリルトランスフェラーゼを作製及び精製する方法 Active JP5984267B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20100171626 EP2415779B1 (en) 2010-08-02 2010-08-02 Method of producing and purifying an active soluble sialyltransferase
EP10171626.4 2010-08-02
PCT/EP2011/063291 WO2012016984A1 (en) 2010-08-02 2011-08-02 Method of producing and purifying an active soluble sialyltransferase

Publications (3)

Publication Number Publication Date
JP2013535207A JP2013535207A (ja) 2013-09-12
JP2013535207A5 true JP2013535207A5 (enExample) 2014-09-11
JP5984267B2 JP5984267B2 (ja) 2016-09-06

Family

ID=42736163

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2013522234A Active JP5984267B2 (ja) 2010-08-02 2011-08-02 活性型の可溶性シアリルトランスフェラーゼを作製及び精製する方法

Country Status (9)

Country Link
US (1) US9222081B2 (enExample)
EP (2) EP2415779B1 (enExample)
JP (1) JP5984267B2 (enExample)
CN (1) CN103154017B (enExample)
CA (1) CA2807397C (enExample)
ES (1) ES2534936T3 (enExample)
MX (1) MX2013001447A (enExample)
PT (1) PT2415779E (enExample)
WO (1) WO2012016984A1 (enExample)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013075740A1 (en) 2011-11-23 2013-05-30 Sanofi Antibody purification method
SG11201505193SA (en) * 2013-02-06 2015-08-28 Agency Science Tech & Res Mixed multifunctional metal affinity surfaces for reducing aggregate content in protein preparations
TWI631132B (zh) 2013-05-06 2018-08-01 賽諾菲公司 用於純化抗體之連續多步驟方法
HK1243329A1 (zh) * 2015-03-31 2018-07-13 Yeda Research And Development Co., Ltd. 谷氨酸草酰乙酸转氨酶1(got1)、其制剂、产生方法及用途
KR101667677B1 (ko) * 2015-05-29 2016-10-19 한국생명공학연구원 재조합 ST6Gal1 단백질을 활성형으로 제조하는 방법
EP3559249A1 (en) 2016-12-21 2019-10-30 H. Hoffnabb-La Roche Ag Method for in vitro glycoengineering of antibodies
MX2019007411A (es) 2016-12-21 2019-08-29 Hoffmann La Roche Reuso de enzimas en glucomanipulacion in vitro de anticuerpos.
KR102229037B1 (ko) 2017-09-04 2021-03-17 89바이오 리미티드 돌연변이 fgf-21 펩티드 접합체 및 이의 용도
WO2019063849A1 (en) * 2017-12-20 2019-04-04 Dsm Ip Assets B.V. PURIFICATION OF A POLYPEPTIDE OF INTEREST
EP3546475A1 (en) 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
KR102362777B1 (ko) * 2018-03-27 2022-02-15 주식회사 녹십자 친화성 크로마토그래피 공정을 포함하는 백일해균 유래 단백질 수득 방법
KR102140531B1 (ko) * 2018-08-07 2020-08-04 (주)휴온스 Gly-Tβ4의 제조방법
DK3613486T3 (da) * 2018-08-24 2021-01-04 Uga Biopharma Gmbh Metode og anlæg til rengøring af epo og/eller et epo-derivat
CN113416243B (zh) * 2021-08-23 2021-12-10 上海盛迪医药有限公司 一种纯化抗体的方法
CN119912505A (zh) * 2025-04-02 2025-05-02 浙江熙正霖生物科技有限公司 一种从发酵液中提纯唾液酸的方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5032519A (en) 1989-10-24 1991-07-16 The Regents Of The Univ. Of California Method for producing secretable glycosyltransferases and other Golgi processing enzymes
WO1995018217A1 (en) 1993-12-24 1995-07-06 The Institute Of Physical And Chemical Research Novel sugar-chain synthetase and process for producing the same
US6280989B1 (en) * 1999-06-17 2001-08-28 Dmitri Kapitonov Sialyltransferases
EP2042196B1 (en) 2001-10-10 2016-07-13 ratiopharm GmbH Remodelling and glycoconjugation of Granulocyte Colony Stimulating Factor (G-CSF)
WO2005055946A2 (en) * 2003-12-03 2005-06-23 Neose Technologies, Inc. Glycopegylated granulocyte colony stimulating factor
US20080206810A1 (en) 2004-06-03 2008-08-28 Neose Technologies, Inc. Truncated St6galnaci Polypeptides and Nucleic Acids
WO2006102652A2 (en) 2005-03-24 2006-09-28 Neose Technologies, Inc. Expression of soluble, active eukaryotic glycosyltransferases in prokaryotic organisms
EP1866427A4 (en) * 2005-03-30 2010-09-01 Novo Nordisk As METHOD OF MANUFACTURING PEPTIDES PRODUCED IN INSECT CELL LINES
WO2007056524A2 (en) 2005-11-09 2007-05-18 Neose Technologies, Inc. St3gal-1/st6galnac-1 chimeras
US8969532B2 (en) * 2006-10-03 2015-03-03 Novo Nordisk A/S Methods for the purification of polypeptide conjugates comprising polyalkylene oxide using hydrophobic interaction chromatography
US20080207487A1 (en) 2006-11-02 2008-08-28 Neose Technologies, Inc. Manufacturing process for the production of polypeptides expressed in insect cell-lines

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