JP5804803B2 - 植物細胞分化促進剤 - Google Patents
植物細胞分化促進剤 Download PDFInfo
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Classifications
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Description
しかしながら、これら方法はいずれも品種や生理的な要因が限定的であると共に、その効果も十分なものとは言い難かった。
[1] 炭素原子数が4〜24のケトール脂肪酸であって、カルボニル基を構成する炭素原子と水酸基が結合した炭素原子がα位の位置にある、炭素原子数が4〜24のケトール脂肪酸を含んでなる植物細胞分化促進剤。
[2] 前記ケトール脂肪酸に、炭素間の二重結合が1〜6か所(ただし、この二重結合数は、ケトール脂肪酸の炭素結合数を超えることはない)存在する、[1]に記載の植物細胞分化促進剤。
[3] 前記ケトール脂肪酸の炭素原子数が18であり、かつ、炭素間の二重結合が2か所存在する、[1]又は[2]に記載の植物細胞分化促進剤。
[4] 前記ケトール脂肪酸が、9−ヒドロキシ−10−オキソ−12(Z),15(Z)−オクタデカジエン酸(以下、KODAと呼ぶ)である、[1]〜[3]のいずれか一項に記載の植物細胞分化促進剤。
[5] 前記植物細胞分化促進剤が、植物のカルスからの不定胚、不定根、又は不定芽の分化を促進する、[1]〜[4]のいずれか一項に記載の植物細胞分化促進剤。
[6] 前記植物細胞分化促進剤が、植物切片からの不定芽又は不定根の分化を促進する、[1]〜[4]のいずれか一項に記載の植物細胞分化促進剤。
[7] 前記植物細胞分化促進剤が、不定胚、不定根、又は不定芽を経た再生植物体への分化を促進する、[1]〜[4]のいずれか一項に記載の植物細胞分化促進剤。
[8] カルスからの再生植物体の作成方法であって、継代培地中で維持しているカルスを再分化培地に移植する3週間前から再分化培地に移植後2週間までの期間に、[1]〜[4]のいずれか一項に記載の植物細胞分化促進剤をカルスに施用する工程を含む、再生植物体の作成方法。
[9] 前記期間が、再分化培地に移植する2週間前から再分化培地に移植後1週間までの期間である、[8]の再生植物体の作成方法。
[10] [9]に記載の再生植物体の作成方法を用いて作成された再生植物体。
[11] [1]〜[4]のいずれか一項に記載の植物細胞分化促進剤を含む、植物培養培地。
植物)の他、シダ類および裸子植物が挙げられる。例えば被子植物のうち、双子葉植物としては、例えば、アサガオ属植物(アサガオ)、ヒルガオ属植物(ヒルガオ、コヒルガオ、ハマヒルガオ)、サツマイモ属植物(グンバイヒルガオ、サツマイモ)、ネナシカズラ属植物(ネナシカズラ、マメダオシ)が含まれるひるがお科植物、ナデシコ属植物、ハコベ属植物、タカネツメクサ属植物、ミミナグサ属植物、ツメクサ属植物、ノミノツヅリ属植物、オオヤマフスマ属植物、ワチガイソウ属植物、ハマハコベ属植物、オオツメクサ属植物、シオツメクサ属植物、マンテマ属植物、センノウ属植物、フシグロ属植物、ナンバンハコベ属植物等のなでしこ科植物をはじめ、もくまもう科植物、どくだみ科植物、こしょう科植物、せんりょう科植物、やなぎ科植物、やまもも科植物、くるみ科植物、かばのき科植物、ぶな科植物、にれ科植物、くわ科植物、いらくさ科植物、かわごけそう科植物、やまもがし科植物、ぼろぼろのき科植物、びゃくだん科植物、やどりぎ科植物、うまのすずくさ科植物、やっこそう科植物、つちとりもち科植物、たで科植物、あかざ科植物、ひゆ科植物、おしろいばな科植物、やまとぐさ科植物、やまごぼう科植物、つるな科植物、すべりひゆ科植物、もくれん科植物、やまぐるま科植物、かつら科植物、すいれん科植物、まつも科植物、きんぽうげ科植物、あけび科植物、めぎ科植物、つづらふじ科植物、ろうばい科植物、くすのき科植物、けし科植物、ふうちょうそう科植物、あぶらな科植物、もうせんごけ科植物、うつぼかずら科植物、べんけいそう科植物、ゆきのした科植物、とべら科植物、まんさく科植物、すずかけのき科植物、ばら科植物、まめ科植物、かたばみ科植物、ふうろそう科植物、あま科植物、はまびし科植物、みかん科植物、にがき科植物、せんだん科植物、ひめはぎ科植物、とうだいぐさ科植物、あわごけ科植物、つげ科植物、がんこうらん科植物、どくうつぎ科植物、うるし科植物、もちのき科植物、にしきぎ科植物、みつばうつぎ科植物、くろたきかずら科植物、かえで科植物、とちのき科植物、むくろじ科植物、あわぶき科植物、つりふねそう科植物、くろうめもどき科植物、ぶどう科植物、ほるとのき科植物、しなのき科植物、あおい科植物、あおぎり科植物、さるなし科植物、つばき科植物、おとぎりそう科植物、みぞはこべ科植物、ぎょりゅう科植物、すみれ科植物、いいぎり科植物、きぶし科植物、とけいそう科植物、しゅうかいどう科植物、さぼてん科植物、じんちょうげ科植物、ぐみ科植物、みそはぎ科植物、ざくろ科植物、ひるぎ科植物、うりのき科植物、のぼたん科植物、ひし科植物、あかばな科植物、ありのとうぐさ科植物、すぎなも科植物、うこぎ科植物、せり科植物、みずき科植物、いわうめ科植物、りょうぶ科植物、いちやくそう科植物、つつじ科植物、やぶこうじ科植物、さくらそう科植物、いそまつ科植物、かきのき科植物、はいのき科植物、えごのき科植物、もくせい科植物、ふじうつぎ科植物、りんどう科植物、きょうちくとう科植物、ががいも科植物、はなしのぶ科植物、むらさき科植物、くまつづら科植物、しそ科植物、なす科植物(なす、トマト等)、ごまのはぐさ科植物、のうぜんかずら科植物、ごま科植物、はまうつぼ科植物、いわたばこ科植物、たぬきも科植物、きつねのまご科植物、はまじんちょう科植物、はえどくそう科植物、おおばこ科植物、あかね科植物、すいかずら科植物、れんぷくそう科植物、おみなえし科植物、まつむしそう科植物、うり科植物、ききょう科植物、きく科植物等を例示することができる。
以下、実施例により本発明を更に詳細に説明するが、これらの実施例は本発明の範囲を何ら制限するものではない。
スパティフィラム(品種Double Take)の培養苗として、旭テクノグラス社製のプラントボックス(内容積300ml)に入れたMS培地(MurashigeとSkoogの培地)にショ糖3%、寒天(和光純薬社、東京、日本国)0.8%を添加しpHを5.8に調整した固体培地上にて、明所(光合成光量子束密度5.7μmole/m2/sec、16時間日長)、25℃の条件下にて継代維持されている培養苗を使用した。
継代培地中に維持しているエンブリオジェニックカルスを、新たな継代培地において継代培養し、1週間(7日)後、2週間(14日)後、3週間(20)後にKODAの3ppm水溶液をカルスに滴下した。継代開始から3週間後に、培養されたカルスを液体の再分化培地に置床し、6週間後に分化した不定胚を回収し(図1)、脱水し、脱水後の不定胚重量を測定した。不定胚を発芽培地に置床し、得られた発芽体の数をカウントした。結果を表1に示す。測定結果から、脱水後の不定胚重量1gあたりの発芽体数を算出した(図2)。対照区に比較し、1週間後処理区では、対照区に比較して増減が見られないが、2週間後処理区で最も増加し、3週間後処理区では2週間後処理区よりも減少した。KODAのカルスに対する不定胚誘導効果は施用時期特異性があると考えられる。
継代培地中に維持しているエンブリオジェニックカルスを、新たな再分化培地に置床し、2週間後に、0.03ppm、0.3ppm、3ppmのKODA水溶液を滴下し、8週間後に正常に再生した植物体の数を記録した。すべての試験区において、植物体に再生しているものの、甚だしいストレス症状(細葉)を有する植物体が多数見られた(図3)。これらの甚だしいストレス症状を示す植物体については、正常に再生した植物体数に計上しなかった。対照区では、ストレス症状を示す植物体の数が多く、結果として、正常に再生した植物体数が少なかった。結果を図4に示す。
Claims (6)
- 炭素原子数が4〜24のケトール脂肪酸であって、カルボニル基を構成する炭素原子と水酸基が結合した炭素原子がα位の位置にある、炭素原子数が4〜24のケトール脂肪酸を含んでなる、カルスからの不定胚への分化促進剤。
- 前記ケトール脂肪酸に、炭素間の二重結合が1〜6か所(ただし、この二重結合数は、ケトール脂肪酸の炭素結合数を超えることはない)存在する、請求項1に記載の分化促進剤。
- 前記ケトール脂肪酸の炭素原子数が18であり、かつ、炭素間の二重結合が2か所存在する、請求項1又は2に記載の分化促進剤。
- 前記ケトール脂肪酸が、9−ヒドロキシ−10−オキソ−12(Z),15(Z)−オクタデカジエン酸である、請求項1〜3のいずれか一項に記載の分化促進剤。
- カルスからの再生植物体の作成方法であって、継代培地中で維持しているカルスを再分化培地に移植する3週間前から移植後2週間までの期間に、カルスに対し請求項1〜4のいずれか一項に記載の分化促進剤を施用する工程を含む、再生植物体の作成方法。
- 請求項1〜4のいずれか一項に記載の分化促進剤を含む、植物培養培地。
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JPH1084802A (ja) * | 1996-09-11 | 1998-04-07 | Science & Tech Agency | ニンジン不定胚の大量培養方法 |
JP3689543B2 (ja) * | 1997-03-04 | 2005-08-31 | 株式会社資生堂 | 花芽形成誘導剤及び花芽形成誘導用キット |
AU6598700A (en) * | 1999-08-23 | 2001-03-19 | Shiseido Company Ltd. | Plant potentiators |
KR20020086687A (ko) * | 2000-03-26 | 2002-11-18 | 가부시키가이샤 시세이도 | 케톨 지방산 유도체 및 식물 성장조정제 |
EP1741338A4 (en) * | 2004-04-27 | 2012-05-09 | Shiseido Co Ltd | PFLANZENBEWURZELUNGINDUKTIONSMITTEL |
JP4177320B2 (ja) * | 2004-11-25 | 2008-11-05 | 株式会社資生堂 | 新規物質のα−ケトール不飽和脂肪酸誘導体及びそれを利用する植物成長調整剤 |
JP4845579B2 (ja) * | 2006-04-24 | 2011-12-28 | 株式会社 資生堂 | 挿し木の発根方法 |
US8519173B2 (en) * | 2009-09-16 | 2013-08-27 | Shiseido Company, Ltd. | Plant growth regulator |
-
2011
- 2011-07-01 JP JP2011147647A patent/JP5804803B2/ja active Active
-
2012
- 2012-06-29 WO PCT/JP2012/066801 patent/WO2013005695A1/ja active Application Filing
- 2012-06-29 EP EP12807142.0A patent/EP2727997A4/en not_active Withdrawn
- 2012-06-29 CN CN201280032438.7A patent/CN103635572A/zh active Pending
- 2012-06-29 US US14/129,785 patent/US20140134735A1/en not_active Abandoned
Also Published As
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CN103635572A (zh) | 2014-03-12 |
EP2727997A1 (en) | 2014-05-07 |
JP2013013356A (ja) | 2013-01-24 |
EP2727997A4 (en) | 2015-04-01 |
WO2013005695A1 (ja) | 2013-01-10 |
US20140134735A1 (en) | 2014-05-15 |
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